ES2005237A6 - Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre - Google Patents

Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre

Info

Publication number
ES2005237A6
ES2005237A6 ES8701523A ES8701523A ES2005237A6 ES 2005237 A6 ES2005237 A6 ES 2005237A6 ES 8701523 A ES8701523 A ES 8701523A ES 8701523 A ES8701523 A ES 8701523A ES 2005237 A6 ES2005237 A6 ES 2005237A6
Authority
ES
Spain
Prior art keywords
glucosylated
haemoglobin
haemolysate
hbalc
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8701523A
Other languages
English (en)
Inventor
Terron Antonio Elduque
Soler Guillermo Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biosystems S A
Original Assignee
Biosystems S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosystems S A filed Critical Biosystems S A
Priority to ES8701523A priority Critical patent/ES2005237A6/es
Priority to EP19870500052 priority patent/EP0293540A1/en
Priority to JP30307087A priority patent/JPS63307364A/ja
Publication of ES2005237A6 publication Critical patent/ES2005237A6/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA LA SEPARACION Y DETERMINACION DEL PORCENTAJE DE HEMOGLOBINA GLUCOSILADA HBALC EN UNA MUESTRA DE SANGRE, A) SE OBTIENE UN HEMOLIZADO A PARTIR DE UNA MUESTRA DE SANGRE, B) SE IMPREGNA CON DICHO HEMOLIZADO UNA RESINA DE INTERCAMBIO CATIONICO DEBIL, C) SE HACE PASAR A TRAVES DE LA RESINA UNA PRIMERA DISOLUCION TAMPON PARA ELUIR DE FORMA SELECTIVA LAS OTRAS HEMOGLOBINAS GLUCOSILADAS HBALA Y HBALB, D) SE HACE PASAR A TRAVES DE LA RESINA UNA SEGUNDA DISOLUCION TAMPON PARA ELUIR DE FORMA SELECTIVA LA HEMOGLOBINA GLUCOSILADA HBALC Y, E) EN SU CASO, SE ANALIZA EN CONTENIDO DE HBALC EN EL ELUIDO RECOGIDO Y SE COMPARA CON LA HEMOGLOBINA TOTAL PRESENTE EN EL HEMOLIZADO, CARACTERIZADO PORQUE LA DISOLUCION QUE SE UTILIZA PARA LA ELUCION SELECTIVA DE LA HEMOGLOBINA GLUCOSILADA HBALC ES UN TAMPON QUE CONTIENE IONES DE UN METAL ALCALINO EN UNA CONCENTRACION COMPRENDIDA ENTRE 0,02 MOLAR Y 0,05 MOLAR YPORQUE LA DISOLUCION QUE SE UTILIZA PARA LA ELUCION PREVIA DE LAS OTRAS HEMOGLOBINAS GLUCOSILADAS HBALA Y HBALB ES UN TAMPON ANALOGO AL ANTERIOR QUE CONTIENE UN REACTIVO ORGANICO POLAR, SOLUBLE EN DISOLUCIONES ACUOSAS, DE CONCENTRACION COMPRENDIDA ENTRE EL 2% Y EL 10% EN VOLUMEN.
ES8701523A 1987-05-25 1987-05-25 Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre Expired ES2005237A6 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES8701523A ES2005237A6 (es) 1987-05-25 1987-05-25 Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre
EP19870500052 EP0293540A1 (en) 1987-05-25 1987-07-30 Procedure for the separation and measurement of Hba1 glycosylated haemoglobin in blood samples
JP30307087A JPS63307364A (ja) 1987-05-25 1987-11-30 血液サンプル中のグルコシルヘモグロビンＨｂＡ１ｃを分離しその百分率を測定する方法およびその実施に使用する装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES8701523A ES2005237A6 (es) 1987-05-25 1987-05-25 Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre

Publications (1)

Publication Number Publication Date
ES2005237A6 true ES2005237A6 (es) 1989-03-01

Family

ID=8251035

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8701523A Expired ES2005237A6 (es) 1987-05-25 1987-05-25 Procedimiento para la separacion y determinacion del porcentaje de hemoglobina glucosilada hbalc en una muestra de sangre

Country Status (3)

Country Link
EP (1) EP0293540A1 (es)
JP (1) JPS63307364A (es)
ES (1) ES2005237A6 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2220369B (en) * 1988-06-10 1993-01-27 Inst Of Child Health Method for testing body fluids by low pressure liquid chromatography
US5543315A (en) * 1992-06-30 1996-08-06 Kyoto Daiichi Kagaku Co., Ltd. Method for stabilizing measurement values by high speed liquid chromatography

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4436820A (en) * 1982-02-01 1984-03-13 Reiter Paul C Method and apparatus for glycosylated hemoglobin separating and measuring fractions
US4389491A (en) * 1982-04-26 1983-06-21 Bio-Rad Laboratories, Inc. Method and kit for chromatographic separation of hemoglobin A1c

Also Published As

Publication number Publication date
JPS63307364A (ja) 1988-12-15
EP0293540A1 (en) 1988-12-07

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000401