EP4676936A1 - Micropeptides and use of the same for inhibiting the growth of parasitic plants - Google Patents

Micropeptides and use of the same for inhibiting the growth of parasitic plants

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Publication number
EP4676936A1
EP4676936A1 EP24708238.1A EP24708238A EP4676936A1 EP 4676936 A1 EP4676936 A1 EP 4676936A1 EP 24708238 A EP24708238 A EP 24708238A EP 4676936 A1 EP4676936 A1 EP 4676936A1
Authority
EP
European Patent Office
Prior art keywords
acid sequence
subject matter
invention concerns
another embodiment
sequence seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24708238.1A
Other languages
German (de)
French (fr)
Inventor
Jean-Philippe COMBIER
Philippe DELAVAULT
Sabine TOURNEUR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Micropep Technologies
Centre National de la Recherche Scientifique CNRS
Institut National de Recherche pour lAgriculture lAlimentation et lEnvironnement
Universite de Toulouse
Nantes Université
Original Assignee
Micropep Technologies
Centre National de la Recherche Scientifique CNRS
Universite de Nantes
Universite Toulouse III Paul Sabatier
Institut National de Recherche pour lAgriculture lAlimentation et lEnvironnement
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Micropep Technologies, Centre National de la Recherche Scientifique CNRS, Universite de Nantes, Universite Toulouse III Paul Sabatier, Institut National de Recherche pour lAgriculture lAlimentation et lEnvironnement filed Critical Micropep Technologies
Publication of EP4676936A1 publication Critical patent/EP4676936A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P13/00—Herbicides; Algicides
    • A01P13/02—Herbicides; Algicides selective
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Definitions

  • a first aspect of the invention relates to a use of a miPEP for inhibiting the growth of a parasitic plant, wherein said miPEP regulates the expression of a miR encoded by the pri- miR, the miPEP of which comes from, said miR and pri-miR being expressed by the parasitic plant.
  • miRs By “miRs”, it means microRNAs or miRNAs which are small non-coding RNAs (ribonucleic acids), about 21 nucleotides in length after maturation, which control expression of target genes at the post-transcriptional level, by degrading the target mRNA or by inhibiting its translation.
  • the miRNAs occur in plants and animals.
  • the target genes are often key genes in developmental processes. For example, they encode transcription factors or proteins of the proteasome.
  • the regulation of expression of the miRs is poorly understood, but it is known in particular that the latter involves, like most coding genes, an RNA polymerase II: this enzyme produces a primary transcript, called “pri-miR”, which is then matured by a protein complex in particular containing the Dicer type enzymes.
  • pre-miR a precursor of miR having a stem-loop secondary structure containing the miR and its complementary sequence miR*. Then the precursor is matured, which leads to formation of a shorter double-stranded RNA containing the miR and the miR*.
  • the miR is then manipulated by the RISC complex, which cleaves the messenger RNA (mRNA) of the target gene or inhibits its translation.
  • mRNA messenger RNA
  • miPEPs By “miPEPs”, it means miR-encoded peptides which are a class of small peptides found to be encoded by pri-miRs through particular open reading frame (or ORF) called specifically miORFs, and that are capable of regulating the expression of a miR encoded by the pri-miR the miPEP of which comes from.
  • ORF open reading frame
  • the impact of a miPEP on the expression of its miR may result in the inhibition of the genes targeted by said miR, however, does not predict its inhibitory effect on the phenotype.
  • the miPEPs in the sense of the present invention must not be understood as necessarily being peptides of small size, as “micro” does not refer to the size of the peptide.
  • miR and “miPEP” may be preceded and/or followed by abbreviations that specifically designate a miR and/or a miPEP and differentiate them from one another.
  • the prefix designates the genus and the species, and the suffix designates the gene from which said miR and/or said miPEP originate.
  • peptides described herein are examples of amino acids described herein:
  • OcmiR and OcmiPEP for example, refer to a miR and a miPEP, respectively, derived from the plant Orobanche cumana (Oc);
  • miR390 and miPEP390 refer to a miR and a miPEP, respectively, of the miR390 gene;
  • OcmiR390 and OcmiPEP390 refer to a miR and a miPEP, respectively, derived from the miR390 gene of the plant Orobanche cumana (Oc).
  • parasitic plants By “parasitic plants”, it means plants that derive some or all of its nutritional requirements from another living plants. It has to be thus understood that parasitic plants are not usual plants and that the knowledge that exists on plants in the common sense cannot be transposed to them mutatis mutandis.
  • parasitic plants particularly belong to Orobanchaceae family and preferably belongs to Orobanche and Phelipanche species among which are Orobanche cumana (Oc) and Phelipanche ramosa (Pr) respectively.
  • Inhibiting the growth of a parasitic plant it means that when an isolated or a chemically- synthetised miPEP is applied to the parasitic plant, it efficiently avoids the parasitic plant to grow.
  • the miPEP positively and specifically activates the transcription of its encoding pri-miRNA. As a result, it indirectly increases the level of its mature miR and induces the downregulation of the expression of the corresponding target genes involved in the growth of said parasitic plant. Consequently, if no molecular processes come to compensate or cancel the impact of said miPEP, the parasitic plant cannot grow or even dies. For this purpose, it is possible to use miPEPs capable of targeting either seed germination or seedling haustoriogenesis.
  • the subject matter of the invention thus concerns the above-described use of a miPEP, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant; both germination and haustoriogenesis being induced by specific biochemical molecules exuded by the roots of the host plant detected by the parasitic plant. Consequently, it has to be understood that the subject matter of the invention concerns as much the above-described use of a miPEP, wherein:
  • said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seed germination of said parasitic plant;
  • said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seedling haustoriogenesis of said parasitic plant;
  • said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seed germination of said parasitic plant and the inhibition of the seedling haustoriogenesis of said parasitic plant.
  • the parasitic plant needs the host plant to germinate and develop (see above), the host plant is not involved in eliminating the parasitic plant.
  • the genetic specificity of the miPEPs of the Invention makes it possible to target only the parasitic plant without impacting the host plant. This is all the more true when considering the inhibition of the seedling haustoriogenesis of the parasitic plant since this mechanism is specific to parasitic plants, host plants lacking it.
  • inhibition of the germination means that when an isolated or a chemically-synthetised miPEP is applied to the parasitic plant, the development of a new individual from a seed placed in favourable conditions is prevented.
  • two different mechanisms may be targeted:
  • the subject matter of the invention thus concerns the abovedescribed use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant. Consequently, it has to be understood that the subject matter of the invention concerns as well the above-described use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant; as the above-described use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and by the inhibition of the metabolic recovery of said parasitic plant.
  • haustoriogenesis By “inhibition of the haustoriogenesis”, it means that when an isolated or a chemically- synthetised miPEP is applied to the parasitic plant, the development of a haustorium which is a rootlike structure that grows into or around another structure to absorb water or nutrients is prevented.
  • a second aspect of the invention relates to a method for inhibiting the growth of a parasitic plant comprising a step of externally (exogenously) introducing a miPEP into said parasitic plant, wherein said miPEP regulates the expression of miR encoded by a pri-miR, the miPEP of which comes from.
  • a step of externally (exogenously) introducing a miPEP into said parasitic plant it means that said miPEP is artificially introduced by external means (watering, spraying, adding a compost, etc.) into the parasitic plant at any stage of its development.
  • the implementation of this step is made when the parasitic plant is in the seed stage or starting to germinate, so as to potentiate the action of the miPEP.
  • the subject matter of the invention thus concerns the above-described method, wherein said miPEP is externally introduced into said parasitic plant:
  • another subject of the invention concerns the above-described method, wherein said miPEP is externally introduced into said parasitic plant in the form of a liquid (watering, spraying) or solid (fertilizer, compost, culture substrate) composition comprising from 10' 9 M to 10' 1 M (or from 10' 9 M to 10' 4 M, or from 10' 4 M to 10' 1 M) of said miPEP or comprising, in particular, 10' 9 M, 10' 8 M, 10' 7 M, 10' 6 M, 10' 5 M, 10' 4 M, 10' 3 M, 10' 2 M or 10' 1 M of said miPEP.
  • a liquid watering, spraying
  • solid (fertilizer, compost, culture substrate) composition comprising from 10' 9 M to 10' 1 M (or from 10' 9 M to 10' 4 M, or from 10' 4 M to 10' 1 M) of said miPEP or comprising, in particular, 10' 9 M, 10' 8 M, 10' 7 M, 10' 6 M, 10' 5 M,
  • another general subject matter of the invention relates to a composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP or comprising, in particular, 10’ 9 M, 10’ 8 M, 10’ 7 M, 10’ 6 M, 10’ 5 M, 10’ 4 M, 10’ 3 M, 10’ 2 M or 10’ 1 M of a miPEP.
  • the composition of the invention does not exist in the natural state, and this is all the more true as such a concentration of miPEP cannot exist within a parasitic plant cell.
  • composition comprising a miPEP means that the composition of the invention comprises at least one miPEP. Consequently, a mixture of miPEPs is conceivable, said miPEPs being able to target a single miR or several miRs depending on the pri-miR(s) from which they are coming from.
  • concentrations refer either to the mixture of miPEPs as such, or to each of the miPEPs of said mixture, said miPEPs being able to be at the same concentration or being able to be at different concentrations among those mentioned above.
  • the subject matter of the invention concerns the abovedescribed composition, said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the composition of the invention may be a phytopharmaceutical composition, an herbicide composition or a coating composition.
  • a solvent may be chosen among: acetone, acetonitrile, acetic acid, formic acid, dimethyl adipate, benzyl acetate, bi-butyl carbonate, dimethyl sulfoxide (DMSO), water, dimethyl glutarate, ammonium hydroxide, iso-butanol, iso-propanol, diethylhexyl lactate, light aromatic naphtha, heavy aromatic naphtha, diethyl succinate and mixtures thereof (e.g., mixture [water; acetic acid]; [acetonitrile; acetic acid], [water, acetonitrile; acetic acid], [water; DMSO], [water; acetonitrile] or [water; ammonium hydroxide]).
  • DMSO dimethyl sulfoxide
  • the latter may in particular be an organic solvent mixture [acetonitrile; acetic acid] in a volume:volume ratio of 99:1 to 1 :99, a mixture [water; DMSO] in a volume:volume ratio of 99:1 to 1 :99, a mixture [water; acetonitrile] in a volume:volume ratio of 99:1 to 1 :99 or a mixture [water; ammonium hydroxide] in a volume:volume ratio of 99:1 to 99.9:0.1.
  • the miPEPs can also be solubilised in a solution comprising 50% acetonitrile, 10% acetic acid and 40% water (v/v/v).
  • a fixing agent may be chosen among: carboxymethyl cellulose (CMC), gum Arabic.
  • CMC carboxymethyl cellulose
  • fixing agents are chemical or natural agents which makes it possible to stick the composition of the invention to a plant seed so as to coat the said plant seed, for instance, to apply and hold the active substance(s) (i.e. miPEPs) on the seed
  • a coating composition comprising a miPEP.
  • another aspect of the invention relates to a coated seed comprising a host plant seed (e.g. sunflower), said host plant seed being coated with the above-described coating composition comprising a miPEP able to inhibit the growth of a parasitic plant (e.g. O. cumana).
  • the coating may be carried out according to the processes conventionally used in the agronomic/food industry and may be obtained by using a material capable of disintegrating in a solvent or in the soil, such as a binder or clay. According to the invention, the coating can be used to impart particular properties to a seed such as improved host plant growth or resistance to certain biotic or abiotic stresses.
  • the targeted miR by the miPEP of the invention is in particular chosen among: miR156a, miR156b, miR156c, miR156d, miR156e, miR156f, miR156g, miR159, miR160a, miR160b, miR160c, miR160d, miR160e, miR164, miR164a, miR164b, miR164c, miR166a, miR166b, miR166c, miR166d, miR166e, miR167a, miR167b, miR167c, miR168, miR169a, miR169b, miR171a, miR171 b, miR171c, miR172a, miR172b, miR172c, miR172d, miR172e, miR172f, miR172g, miR319a, miR319b, miR390, miR393a, miR
  • the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • the inventors found that the use of miPEP319a specifically directed against miR319a expressed by parasitic plants, was able to inhibit the growth of both O. cumana and P. ramosa. In other words, with this tool, the inventors have developed a universal and easy- to-use tool against these parasitic plants.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said parasitic plant belongs to Orobanchaceae family.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346.
  • percentage of identity it means the percentage determined by direct comparison of two oligonucleotide (or amino acid) sequences by determining the number of identical nucleotides (or residues) between the two sequences, dividing by the number of nucleotides (or residues) in the longer of the two sequences and multiplying by 100.
  • the expression “at least 80%” also means that the aforementioned percentage identity is at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100%.
  • this definition applies to all embodiments of the invention.
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant:
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP319a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said parasitic plant belongs to Orobanchaceae family.
  • the subject matter of the invention concerns the abovedescribed method, wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346.
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 1 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 3 or 4 (present on the pre-miR156a of sequence SEQ ID NO: 2).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6 (encoded by the miORF156a of sequence SEQ ID NO: 5).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156a into said parasitic plant, wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 1 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 3 or 4 (present on the pre-miR156a of sequence SEQ ID NO: 2).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6 (encoded by the miORF156a of sequence SEQ ID NO: 5).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP156a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 7, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 9 or 10 (present on the pre-miR156b of sequence SEQ ID NO: 8).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 19, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 21 or 22 (present on the pre-miR156d of sequence SEQ ID NO: 20).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 19, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 21 or 22 (present on the pre-miR156d of sequence SEQ ID NO: 20).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 30 (encoded by the miORF156e of sequence SEQ ID NO: 29).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP156e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 30, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156f.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP159, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEPI 59, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEPI 59 into said parasitic plant, wherein said miPEPI 59 regulates the expression of miR159 encoded by a pri- miR159.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEPI 59 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP159 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP159.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 37, in particular wherein said miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 39 or 40 (present on the pre-miR159 of sequence SEQ ID NO: 38).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP159 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 42 (encoded by the miORF159 of sequence SEQ ID NO: 41).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP159 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 42, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP160a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160a regulates the expression of miR160a encoded by a pri-miR160a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP160a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160a, wherein said miPEP160a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48 (encoded by the miQRF160a of sequence SEQ ID NO: 47).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160a into said parasitic plant, wherein said miPEP160a regulates the expression of miR160a encoded by a pri-miR160a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP160a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 43, in particular wherein said miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 45 or 46 (present on the pre-miR160a of sequence SEQ ID NO: 44).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48 (encoded by the miQRF160a of sequence SEQ ID NO: 47).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP160a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP160b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160b regulates the expression of miR160b encoded by a pri-miR160b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP160b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said pri-miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 49, in particular wherein said miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 51 or 52 (present on the pre-miR160b of sequence SEQ ID NO: 50).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said miPEP160b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54 (encoded by the miQRF160b of sequence SEQ ID NO: 53).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160b into said parasitic plant, wherein said miPEP160b regulates the expression of miR160b encoded by a pri-miR160b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP160b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 49, in particular wherein said miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 51 or 52 (present on the pre-miR160b of sequence SEQ ID NO: 50).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54 (encoded by the miQRF160b of sequence SEQ ID NO: 53).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP160b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP160c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said pri-miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 55, in particular wherein said miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 57 or 58 (present on the pre-miR160c of sequence SEQ ID NO: 56).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said miPEP160c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59.
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160c into said parasitic plant, wherein said miPEP160c regulates the expression of miR160c encoded by a pri-miR160c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 55, in particular wherein said miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 57 or 58 (present on the pre-miR160c of sequence SEQ ID NO: 56).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59.
  • the subject matter of the invention concerns the abovedescribed composition
  • a miPEP160c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP160d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160d regulates the expression of miR160d encoded by a pri-miR160d.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP160d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said pri-miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 60, in particular wherein said miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 62 or 63 (present on the pre-miR160d of sequence SEQ ID NO: 61).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said miPEP160d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65 (encoded by the miQRF160d of sequence SEQ ID NO: 64).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160d into said parasitic plant, wherein said miPEP160d regulates the expression of miR160d encoded by a pri-miR160d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP160d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 60, in particular wherein said miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 62 or 63 (present on the pre-miR160d of sequence SEQ ID NO: 61).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65 (encoded by the miQRF160d of sequence SEQ ID NO: 64).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP160d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP160e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160e regulates the expression of miR160e encoded by a pri-miR160e.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP160e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said pri-miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 66, in particular wherein said miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 68 or 69 (present on the pre-miR160e of sequence SEQ ID NO: 67).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said miPEP160e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 (encoded by the miQRF160e of sequence SEQ ID NO: 70).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160e into said parasitic plant, wherein said miPEP160e regulates the expression of miR160e encoded by a pri-miR160e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP160e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 66, in particular wherein said miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 68 or 69 (present on the pre-miR160e of sequence SEQ ID NO: 67).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 (encoded by the miQRF160e of sequence SEQ ID NO: 70).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP160e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 , in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP164 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP164 regulates the expression of miR164 encoded by a pri-miR164.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP164, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164, wherein said miPEP164 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 76.
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP164 into said parasitic plant, wherein said miPEP164 regulates the expression of miR164 encoded by a pri- miR164.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 77, in particular wherein said miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 79 or 80 (present on the pre-miR166a of sequence SEQ ID NO: 78).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP166b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said pri-miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 83, in particular wherein said miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 85 or 86 (present on the pre-miR166b of sequence SEQ ID NO: 84).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said miPEP166b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 88 (encoded by the miORF166b of sequence SEQ ID NO: 87).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166b into said parasitic plant, wherein said miPEP166b regulates the expression of miR166b encoded by a pri-miR166b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 83, in particular wherein said miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 85 or 86 (present on the pre-miR166b of sequence SEQ ID NO: 84).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 88 (encoded by the miORF166b of sequence SEQ ID NO: 87).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166c into said parasitic plant, wherein said miPEP166c regulates the expression of miR166c encoded by a pri-miR166c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP166c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 89, in particular wherein said miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 91 or 92 (present on the pre-miR166c of sequence SEQ ID NO: 90).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 94 (encoded by the miORF166c of sequence SEQ ID NO: 93).
  • the subject matter of the invention concerns the abovedescribed composition
  • a miPEP166c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 94, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP166d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166d regulates the expression of miR166d encoded by a pri-miR166d.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP166d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said pri-miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 95, in particular wherein said miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 97 or 98 (present on the pre-miR166d of sequence SEQ ID NO: 96).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said miPEP166d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100 (encoded by the miORF166d of sequence SEQ ID NO: 99).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166d into said parasitic plant, wherein said miPEP166d regulates the expression of miR166d encoded by a pri-miR166d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP166d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 95, in particular wherein said miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 97 or 98 (present on the pre-miR166d of sequence SEQ ID NO: 96).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100 (encoded by the miORF166d of sequence SEQ ID NO: 99).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP166d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP166e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166e regulates the expression of miR166e encoded by a pri-miR166e.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP166e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said pri-miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 101 , in particular wherein said miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 103 or 104 (present on the pre-miR166e of sequence SEQ ID NO: 102).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said miPEP166e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106 (encoded by the miORF166e of sequence SEQ ID NO: 105).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166e into said parasitic plant, wherein said miPEP166e regulates the expression of miR166e encoded by a pri-miR166e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP166e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 101 , in particular wherein said miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 103 or 104 (present on the pre-miR166e of sequence SEQ ID NO: 102).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106 (encoded by the miORF166e of sequence SEQ ID NO: 105).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP166e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP167a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167a regulates the expression of miR167a encoded by a pri-miR167a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP167a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said pri-miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 107, in particular wherein said miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 109 or 110 (present on the pre-miR167a of sequence SEQ ID NO: 108).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said miPEP167a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112 (encoded by the miORF167a of sequence SEQ ID NO: 111).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167a into said parasitic plant, wherein said miPEP167a regulates the expression of miR167a encoded by a pri-miR167a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP167a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 107, in particular wherein said miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 109 or 110 (present on the pre-miR167a of sequence SEQ ID NO: 108).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112 (encoded by the miORF167a of sequence SEQ ID NO: 111).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP167a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP167b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167b regulates the expression of miR167b encoded by a pri-miR167b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP167b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said pri-miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 113, in particular wherein said miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 115 or 116 (present on the pre-miR167b of sequence SEQ ID NO: 114).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said miPEP167b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118 (encoded by the miORF167b of sequence SEQ ID NO: 117).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167b into said parasitic plant, wherein said miPEP167b regulates the expression of miR167b encoded by a pri-miR167b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP167b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 113, in particular wherein said miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 115 or 116 (present on the pre-miR167b of sequence SEQ ID NO: 114).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118 (encoded by the miORF167b of sequence SEQ ID NO: 117).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP167b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP167c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167c regulates the expression of miR167c encoded by a pri-miR167c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP167c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said pri-miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 119, in particular wherein said miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 121 or 122 (present on the pre-miR167c of sequence SEQ ID NO: 120).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said miPEP167c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 124 (encoded by the miORF167c of sequence SEQ ID NO: 123).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167c into said parasitic plant, wherein said miPEP167c regulates the expression of miR167c encoded by a pri-miR167c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP167c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 119, in particular wherein said miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 121 or 122 (present on the pre-miR167c of sequence SEQ ID NO: 120).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 124 (encoded by the miORF167c of sequence SEQ ID NO: 123).
  • the subject matter of the invention concerns the abovedescribed use of a miPEP168 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP168 regulates the expression of miR168 encoded by a pri-miR168.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP168, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said pri-miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 125, in particular wherein said miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 127 or 128 (present on the pre-miR168 of sequence SEQ ID NO: 126).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said miPEP168 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130 (encoded by the miORF168 of sequence SEQ ID NO: 129).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP168 into said parasitic plant, wherein said miPEP168 regulates the expression of miR168 encoded by a pri- miR168.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP168.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 125, in particular wherein said miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 127 or 128 (present on the pre-miR168 of sequence SEQ ID NO: 126).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130 (encoded by the miORF168 of sequence SEQ ID NO: 129).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP168 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP169a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP169a regulates the expression of miR169a encoded by a pri-miR169a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP169a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said pri-miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 131 , in particular wherein said miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 133 or 134 (present on the pre-miR169a of sequence SEQ ID NO: 132).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said miPEP169a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136 (encoded by the miORF169a of sequence SEQ ID NO: 135).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP169a into said parasitic plant, wherein said miPEP169a regulates the expression of miR169a encoded by a pri-miR169a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP169a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 131 , in particular wherein said miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 133 or 134 (present on the pre-miR169a of sequence SEQ ID NO: 132).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136 (encoded by the miORF169a of sequence SEQ ID NO: 135).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP169a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP169b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP169b regulates the expression of miR169b encoded by a pri-miR169b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP169b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said pri-miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 137, in particular wherein said miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 139 or 140 (present on the pre-miR169b of sequence SEQ ID NO: 138).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said miPEP169b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142 (encoded by the miORF169b of sequence SEQ ID NO: 141).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP169b into said parasitic plant, wherein said miPEP169b regulates the expression of miR169b encoded by a pri-miR169b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP169b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 137, in particular wherein said miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 139 or 140 (present on the pre-miR169b of sequence SEQ ID NO: 138).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142 (encoded by the miORF169b of sequence SEQ ID NO: 141).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP169b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP171a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171a regulates the expression of miR171a encoded by a pri-miR171a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP171a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171a, wherein said miPEP171a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 148 (encoded by the miORF171a of sequence SEQ ID NO: 147).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP171a into said parasitic plant, wherein said miPEP171a regulates the expression of miR171a encoded by a pri-miR171a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP171a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 143, in particular wherein said miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 145 or 146 (present on the pre-miR171a of sequence SEQ ID NO: 144).
  • the subject matter of the invention concerns the abovedescribed use of a miPEP171 b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171 b regulates the expression of miR171 b encoded by a pri-miR171 b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171 b, wherein said pri-miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 149, in particular wherein said miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 151 or 152 (present on the pre-miR171 b of sequence SEQ ID NO: 150).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171 b, wherein said miPEP171b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154 (encoded by the miORF171b of sequence SEQ ID NO: 153).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O.
  • cumana comprising a step of externally introducing a miPEP171 b into said parasitic plant, wherein said miPEP171b regulates the expression of miR171 b encoded by a pri-miR171b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171 b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP171b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 149, in particular wherein said miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 151 or 152 (present on the pre-miR171 b of sequence SEQ ID NO: 150).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154 (encoded by the miORF171b of sequence SEQ ID NO: 153).
  • the subject matter of the invention concerns the abovedescribed composition
  • a miPEP171b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP171c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171c regulates the expression of miR171c encoded by a pri-miR171c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP171c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said pri-miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 155, in particular wherein said miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 157 or 158 (present on the pre-miR171c of sequence SEQ ID NO: 156).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said miPEP171c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160 (encoded by the miORF171c of sequence SEQ ID NO: 159).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP171c into said parasitic plant, wherein said miPEP171c regulates the expression of miR171c encoded by a pri-miR171c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP171c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 155, in particular wherein said miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 157 or 158 (present on the pre-miR171c of sequence SEQ ID NO: 156).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160 (encoded by the miORF171c of sequence SEQ ID NO: 159).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP171c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 161 , in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 163 or 164 (present on the pre-miR172a of sequence SEQ ID NO: 162).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said miPEP172a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 166 (encoded by the miORF172a of sequence SEQ ID NO: 165).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172a into said parasitic plant, wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 161 , in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 163 or 164 (present on the pre-miR172a of sequence SEQ ID NO: 162).
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 167, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 169 or 170 (present on the pre-miR172e of sequence SEQ ID NO: 168).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172 (encoded by the miORF172e of sequence SEQ ID NO: 171).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172e into said parasitic plant, wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 167, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 169 or 170 (present on the pre-miR172e of sequence SEQ ID NO: 168).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172 (encoded by the miORF172e of sequence SEQ ID NO: 171).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP172e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 173, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 175 or 176 (present on the pre-miR172b of sequence SEQ ID NO: 174).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 173, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 175 or 176 (present on the pre-miR172b of sequence SEQ ID NO: 174).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 178 (encoded by the miORF172b of sequence SEQ ID NO: 177).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 179, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 181 or 182 (present on the pre-miR172c of sequence SEQ ID NO: 180).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172c into said parasitic plant, wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 179, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 181 or 182 (present on the pre-miR172c of sequence SEQ ID NO: 180).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 184 (encoded by the miORF172c of sequence SEQ ID NO: 183).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 185, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 187 or 188 (present on the pre-miR172d of sequence SEQ ID NO: 186).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190 (encoded by the miORF172d of sequence SEQ ID NO: 189).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172d into said parasitic plant, wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 185, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 187 or 188 (present on the pre-miR172d of sequence SEQ ID NO: 186).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190 (encoded by the miORF172d of sequence SEQ ID NO: 189).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP172d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 191 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193 or 194 (present on the pre-miR319a of sequence SEQ ID NO: 192).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196 (encoded by the miORF319a of sequence SEQ ID NO: 195).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP319a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 191 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193 or 194 (present on the pre-miR319a of sequence SEQ ID NO: 192).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196 (encoded by the miORF319a of sequence SEQ ID NO: 195).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP319b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP319b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 197, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 199 or 200 (present on the pre-miR319b of sequence SEQ ID NO: 198).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202 (encoded by the miORF319b of sequence SEQ ID NO: 201).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP319b into said parasitic plant, wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP319b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 197, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 199 or 200 (present on the pre-miR319b of sequence SEQ ID NO: 198).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202 (encoded by the miORF319b of sequence SEQ ID NO: 201).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP319b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP390 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP390 regulates the expression of miR390 encoded by a pri-miR390.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP390, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said pri-miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 203, in particular wherein said miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 205 or 206 (present on the pre-miR390 of sequence SEQ ID NO: 204).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said miPEP390 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208 (encoded by the miQRF390 of sequence SEQ ID NO: 207).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP390 into said parasitic plant, wherein said miPEP390 regulates the expression of miR390 encoded by a pri- miR390.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP390.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 203, in particular wherein said miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 205 or 206 (present on the pre-miR390 of sequence SEQ ID NO: 204).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208 (encoded by the miQRF390 of sequence SEQ ID NO: 207).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP390 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP393a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP393a regulates the expression of miR393a encoded by a pri-miR393a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP393a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said pri-miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 209, in particular wherein said miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 211 or 212 (present on the pre-miR393a of sequence SEQ ID NO: 210).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said miPEP393a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214 (encoded by the miORF393a of sequence SEQ ID NO: 213).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP393a into said parasitic plant, wherein said miPEP393a regulates the expression of miR393a encoded by a pri-miR393a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP393a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 209, in particular wherein said miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 211 or 212 (present on the pre-miR393a of sequence SEQ ID NO: 210).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214 (encoded by the miORF393a of sequence SEQ ID NO: 213).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP393a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP393b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said pri-miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 215, in particular wherein said miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 217 or 218 (present on the pre-miR393b of sequence SEQ ID NO: 216).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said miPEP393b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220 (encoded by the miORF393b of sequence SEQ ID NO: 219).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP393b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 215, in particular wherein said miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 217 or 218 (present on the pre-miR393b of sequence SEQ ID NO: 216).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220 (encoded by the miORF393b of sequence SEQ ID NO: 219).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP393b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP394 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP394 regulates the expression of miR394 encoded by a pri-miR394.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP394, wherein said pri-miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 221 , in particular wherein said miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 223 or 224 (present on the pre-miR394 of sequence SEQ ID NO: 222).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP394, wherein said miPEP394 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226 (encoded by the miORF394 of sequence SEQ ID NO: 225).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP394 into said parasitic plant, wherein said miPEP394 regulates the expression of miR394 encoded by a pri- miR394.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 221 , in particular wherein said miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 223 or 224 (present on the pre-miR394 of sequence SEQ ID NO: 222).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP394 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226 (encoded by the miORF394 of sequence SEQ ID NO: 225).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP394 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP395 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP395 regulates the expression of miR395 encoded by a pri-miR395.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP395, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said pri-miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 227, in particular wherein said miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 229 or 230 (present on the pre-miR395 of sequence SEQ ID NO: 228).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said miPEP395 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232 (encoded by the miORF395 of sequence SEQ ID NO: 231).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP395 into said parasitic plant, wherein said miPEP395 regulates the expression of miR395 encoded by a pri- miR395.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP395.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 227, in particular wherein said miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 229 or 230 (present on the pre-miR395 of sequence SEQ ID NO: 228).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232 (encoded by the miORF395 of sequence SEQ ID NO: 231).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP395 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP396a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP396a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 233, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 235 or 236 (present on the pre-miR396a of sequence SEQ ID NO: 234).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238 (encoded by the miORF396a of sequence SEQ ID NO: 237).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396a into said parasitic plant, wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP396a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 233, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 235 or 236 (present on the pre-miR396a of sequence SEQ ID NO: 234).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238 (encoded by the miORF396a of sequence SEQ ID NO: 237).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP396a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP396b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP396b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 239, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 241 or 242 (present on the pre-miR396b of sequence SEQ ID NO: 240).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244 (encoded by the miORF396b of sequence SEQ ID NO: 243).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396b into said parasitic plant, wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP396b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 239, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 241 or 242 (present on the pre-miR396b of sequence SEQ ID NO: 240).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244 (encoded by the miORF396b of sequence SEQ ID NO: 243).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP396b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP396c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396c regulates the expression of miR396c encoded by a pri-miR396c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP396c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said pri-miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 245, in particular wherein said miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 247 or 248 (present on the pre-miR396c of sequence SEQ ID NO: 246).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said miPEP396c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250 (encoded by the miORF396c of sequence SEQ ID NO: 249).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396c into said parasitic plant, wherein said miPEP396c regulates the expression of miR396c encoded by a pri-miR396c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP396c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 245, in particular wherein said miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 247 or 248 (present on the pre-miR396c of sequence SEQ ID NO: 246).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250 (encoded by the miORF396c of sequence SEQ ID NO: 249).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP396c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
  • the invention includes their combination. In particular, the following uses are described:
  • miPEP for inhibiting the metabolic recovery of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR156a, miR166d, miR319a, miR393b and miR396b;
  • miPEP for inhibiting the radicle protrusion of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR164, miR395, miR156a, miR156b, miR156c, miR156d, miR156e, miR156f, miR156g, miR160a, miR160b, miR160c, miR160d, miR166d, miR167a, miR167b, miR169a, miR169b, miR171 b, miR171c, miR172a, miR172b, miR172c, miR172d, miR172e, miR172g, miR319a, miR319b, miR393a, miR393b, miR396a, miR396b and miR396c;
  • miPEP for inhibiting the metabolic recovery and (or) the radicle protrusion of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR156a, miR166d, miR319a, miR393b and miR396b;
  • miPEP for inhibiting the haustoriogenesis of parasitic plant seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR172c, miR172d, miR319a and miR319b;
  • miPEP for inhibiting the metabolic recovery and (or) the haustoriogenesis of parasitic plant seed and (or) seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of miR319a;
  • miPEP for inhibiting the radicle protrusion and (or) the haustoriogenesis of parasitic plant seed and (or) seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR172c and miR319a; and
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 251 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 253 or 254 (present on the pre-miR156a of sequence SEQ ID NO: 252).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 251 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 253 or 254 (present on the pre-miR156a of sequence SEQ ID NO: 252).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 256 (encoded by the miORF156a of sequence SEQ ID NO: 255).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP156a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 256, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 257, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 259 (present on the pre-miR156b of sequence SEQ ID NO: 258).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 (encoded by the miORF156b of sequence SEQ ID NO: 260).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156b into said parasitic plant, wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 257, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 259 (present on the pre-miR156b of sequence SEQ ID NO: 258).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 (encoded by the miORF156b of sequence SEQ ID NO: 260).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP156b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 262, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 264 (present on the pre-miR156c of sequence SEQ ID NO: 263).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156c into said parasitic plant, wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 262, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 264 (present on the pre-miR156c of sequence SEQ ID NO: 263).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 266 (encoded by the miORF156c of sequence SEQ ID NO: 265).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP156c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 266, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156d for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 267, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 269 (present on the pre-miR156d of sequence SEQ ID NO: 268).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 (encoded by the miORF156d of sequence SEQ ID NO: 270).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156d into said parasitic plant, wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156d.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 267, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 269 (present on the pre-miR156d of sequence SEQ ID NO: 268).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 (encoded by the miORF156d of sequence SEQ ID NO: 270).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP156d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156e for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP156e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 272, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 274 or 275 (present on the pre-miR156e of sequence SEQ ID NO: 273).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277 (encoded by the miORF156e of sequence SEQ ID NO: 276).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156e into said parasitic plant, wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP156e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 272, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 274 or 275 (present on the pre-miR156e of sequence SEQ ID NO: 273).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277 (encoded by the miORF156e of sequence SEQ ID NO: 276).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP156e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP156f for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156f regulates the expression of miR156f encoded by a pri-miR156f.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said pri-miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 278, in particular wherein said miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 280 or 281 (present on the pre-miR156f of sequence SEQ ID NO: 279).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said miPEP156f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 283 (encoded by the miORF156f of sequence SEQ ID NO: 282).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP156g, wherein said pri-miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 284, in particular wherein said miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 286 or 287 (present on the pre-miR156g of sequence SEQ ID NO: 285).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 289 (encoded by the miORF156g of sequence SEQ ID NO: 288).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP156g having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 289, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164a, wherein said miPEP164a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295 (encoded by the miORF164a of sequence SEQ ID NO: 294).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164a into said parasitic plant, wherein said miPEP164a regulates the expression of miR164a encoded by a pri-miR164a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP164a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 290, in particular wherein said miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 292 or 293 (present on the pre-miR164a of sequence SEQ ID NO: 291).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295 (encoded by the miORF164a of sequence SEQ ID NO: 294).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP164a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP164b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP164b regulates the expression of miR164b encoded by a pri-miR164b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP164b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said pri-miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 296, in particular wherein said miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 298 or 299 (present on the pre-miR164b of sequence SEQ ID NO: 297).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said miPEP164b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 301 (encoded by the miORF164b of sequence SEQ ID NO: 300).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164b into said parasitic plant, wherein said miPEP164b regulates the expression of miR164b encoded by a pri-miR164b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP164b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 296, in particular wherein said miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 298 or 299 (present on the pre-miR164b of sequence SEQ ID NO: 297).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP164b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 301 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP164c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP164c regulates the expression of miR164c encoded by a pri-miR164c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP164c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said pri-miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 302, in particular wherein said miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 304 (present on the pre-miR164c of sequence SEQ ID NO: 303).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said miPEP164c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 306 (encoded by the miORF164c of sequence SEQ ID NO: 305).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164c into said parasitic plant, wherein said miPEP164c regulates the expression of miR164c encoded by a pri-miR164c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP164c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP172a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 317, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 318, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 320 (present on the pre-miR172b of sequence SEQ ID NO: 319).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322 (encoded by the miORF172b of sequence SEQ ID NO: 321).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172b into said parasitic plant, wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 318, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 320 (present on the pre-miR172b of sequence SEQ ID NO: 319).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322 (encoded by the miORF172b of sequence SEQ ID NO: 321).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP172b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 323, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 325 or 326 (present on the pre-miR172c of sequence SEQ ID NO: 324).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 328 (encoded by the miORF172c of sequence SEQ ID NO: 327).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172c into said parasitic plant, wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 323, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 325 or 326 (present on the pre-miR172c of sequence SEQ ID NO: 324).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 328 (encoded by the miORF172c of sequence SEQ ID NO: 327).
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172d for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172g for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172g regulates the expression of miR172g encoded by a pri-miR172g.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172g, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172g, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172g, wherein said pri-miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 335, in particular wherein said miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 337 or 338 (present on the pre-miR172g of sequence SEQ ID NO: 336).
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 335, in particular wherein said miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 337 or 338 (present on the pre-miR172g of sequence SEQ ID NO: 336).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 340 (encoded by the miORF172g of sequence SEQ ID NO: 339).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP172g having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 340, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 343 or 344 (present on the pre-miR319a of sequence SEQ ID NO: 342).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346 (encoded by the miORF319a of sequence SEQ ID NO: 345).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
  • Phelipanche species in particular P. ramosa
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 343 or 344 (present on the pre-miR319a of sequence SEQ ID NO: 342).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346 (encoded by the miORF319a of sequence SEQ ID NO: 345).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP319b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP319b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 347, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 349 or 350 (present on the pre-miR319b of sequence SEQ ID NO: 348).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352 (encoded by the miORF319b of sequence SEQ ID NO: 351).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP319b into said parasitic plant, wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP319b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 347, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 349 or 350 (present on the pre-miR319b of sequence SEQ ID NO: 348).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352 (encoded by the miORF319b of sequence SEQ ID NO: 351).
  • the subject matter of the invention concerns the abovedescribed composition
  • a composition comprising from 10' 9 M to 10' 1 M of a miPEP319b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP172e for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP172e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 353, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 355 or 356 (present on the pre-miR172e of sequence SEQ ID NO: 354).
  • the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358 (encoded by the miORF172e of sequence SEQ ID NO: 357).
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172e into said parasitic plant, wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10' 9 M to 10' 1 M of said miPEP172e.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 353, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 355 or 356 (present on the pre-miR172e of sequence SEQ ID NO: 354).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358 (encoded by the miORF172e of sequence SEQ ID NO: 357).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP172e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP396a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP396a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • ramosa comprising a step of externally introducing a miPEP396a into said parasitic plant, wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
  • the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 359, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 361 or 362 (present on the pre-miR396a of sequence SEQ ID NO: 360).
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 364 (encoded by the miORF396a of sequence SEQ ID NO: 363).
  • the subject matter of the invention concerns the abovedescribed composition comprising from 10' 9 M to 10' 1 M of a miPEP396a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 364, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
  • the subject matter of the invention concerns the abovedescribed use of a miPEP396b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
  • the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
  • the subject matter of the invention concerns the above-described use of the miPEP396b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
  • the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP396b into said parasitic plant, wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
  • the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 370 (encoded by the miORF396b of sequence SEQ ID NO: 369).
  • Sunflowers were cultivated in a photoperiod of 16 h day / 8 h night and watered 3 times per week with 10 mL of half Long Ashton watering solution containing (control) or not 1 pM of miPEP. A watering complement was done 7 h after miPEP treatments with a half Long Ashton solution. The broomrape attachment count was conducted 5 weeks after sowing.
  • Target gene expression data from seeds treated or not with ( ⁇ )-GR24 were subjected to pairwise comparisons (T ukey-HSD) between treatment and negative control (solvent treatment with 0.1 % (v/v) of acetonitrile) and with a confidence interval of 99.9 % and 99 %.
  • the target genes of miRNA conserved in A. thaliana were searched in the genome of O. cumana using blast modules.
  • the miRNA binding sites were searched in the transcripts of the target genes by sequence alignment. Only target genes whose binding site had a maximum of five mismatches and no more than two in a row were retained. Additional verification was performed on O. cumana target gene sequences using psRNATarget software (Dai et al., 2018, Nucleic Acids Research 46(W1): W49-w54) by blasting A. thaliana miRNAs. By applying this strategy, 37 mRNA targets for 13 of the 16 identified miRNAs families were identified (Table 2).
  • the 0.1 nM ( ⁇ )-GR24 concentration is the one allowing the highest inhibitory activity of the tested miPEPs.
  • miPEP159, miPEP160a,b,c,d miPEP164, miPEP169b, miPEP172a,b, miPEP319a, and miPEP393b
  • miPEP159, mir160, mir164, mir169, mir172, mir319, and mir393 exhibits inhibitory activity on radicle protrusion while only miPEP319a and miPEP393b inhibit both metabolic activity recovery and radicle protrusion, whatever the ( ⁇ )-GR24 concentration.
  • miPEP319a has the strongest inhibitory activity on the germination of O. cumana seeds.
  • miR319a and miR319b are two different pri-miRNAs producing the same mature miRNA. It is interesting to note that miP319b has no effect on this process demonstrating the miRNA-specific nature of the mode of action of miPEPs. The impact of a miPEP on the expression of its miR may result in the inhibition of the genes targeted by said miR, however, does not predict its inhibitory effect on the phenotype.
  • miPEP319a exhibits a strong inhibitory effect on P. ramosa seed germination whatever the ( ⁇ )-GR24 concentration, whereas miPEP319b had no effect.
  • This result clearly demonstrates the versatile nature of one of the miR319-related miPEPs in its ability to inhibit seed germination in broomrapes. It is interesting to note the miRNA-specific nature of the action of miPEPs since of the two members of the miPEP319 family, one is strongly inhibitory while the second is inactive in both species. This was confirmed by performing germination assays with varying concentrations of ( ⁇ )-GR24 and miPEP (Fig. 4).
  • miPEP319a At a concentration of 100 pM, miPEP319a induced a total inhibition of radicle protrusion in both broomrape species regardless of ( ⁇ )- GR24 concentration, accompanied by a partial or total inhibition of metabolic recovery in O. cumana and P. ramosa, respectively.
  • miPEP319b had only a slight effect only at low concentration of ( ⁇ )-GR24 and high concentration of peptide. In order to verify that the effects induced by these miPEPs during the germination of O.
  • Root exudates from the sunflower accession 2603 known to be susceptible to broomrape, were also used in order to simulate as much as possible the natural conditions of germination induction of O. cumana seeds by the host plant. Indeed, these root exudates are likely to contain the two types of germination stimulants, strigolatones and sesquiterpene lactones (Ueno et al., 2014, Phytochemistry 108: 122-128). Three successive dilutions to the tenth in root exudate were used. Exudates induce a response similar to that of ( ⁇ )-GR24 or DCL following treatment with miPEP319a (Fig. 10). The inhibitory effect on radicle protrusion and metabolic recovery is strong at the highest miPEP concentration. In contrast, miPEP319b does not induce significant changes.
  • the effect of miPEP319 was also characterized on another race of O. cumana to confirm that the germination inhibitory effects observed for miPEP319a are not race-specific.
  • the OclN23 population was collected in Spain in the Guadalquivir Valley and corresponds to the race F, while the French Oc49 population is a race E. ( ⁇ )-GR24 was not used on this seed lot because they germinate poorly with this synthetic stimulant, making visualization of a potential inhibitory effect of miPEP very difficult. Inhibition of radicle protrusion is observed for all concentrations of miPEP319a and DCL used (Fig. 10).
  • ( ⁇ )-GR24 leads to an accumulation of the target gene mRNAs as early as 6 h after treatment and which is maintained or increases up to 48 h with the exception of OcTCP2 which shows a transient upregulation.
  • application of the active synthetic miPEP319a induces a significative decrease of the corresponding mRNA, which is not the case with the inactive miPEP166c.
  • pri-miR319a a decrease in accumulation is observed 6 h after ( ⁇ )-GR24 addition, which coincides interestingly with the upregulation of all four target genes.
  • 0.1 pM ( ⁇ )-GR24 induces a strong and rapid accumulation of CYP707A 1 mRNA (Fig. 6a).
  • a potential effect of miPEP319a seed germination was induced with a lower ( ⁇ )- GR24 concentration of 0.1 nM explaining a global lower CYP707A 1 expression level (Fig. 6b).
  • Treatment of O. cumana seeds with synthetic miPEP319a decreases the accumulation of CYP707A 1 3h after application suggesting that expression of this gene is directly or indirectly under the control of transcription factors, themselves under the control of miR319.
  • miPEP319a or miPEP319b induces a significant decrease in the number of broomrape attachments on sunflowers, compared to sunflowers co- cultured with O. cumana seeds watered with the nutrient solution alone (Fig. 7). Both miPEPs thus reduce sunflower infestation by O. cumana under these pot culture conditions. In the case of miPEP319a, this reduction can be attributed to the inhibitory effect of the synthetic peptide on the seed germination rate then significantly reducing the number of parasites in the rhizospere. Concerning miPEP319b, the observed reduction was less expected since this peptide has never shown any inhibitory effect on O. cumana germination.
  • miPEP319a induces inhibition of seed germination, marked by activation of pri-miR319a expression and repression of expression of both OcMYB33a and OcMYB33b genes (Fig. 5). Both miPEP treatments of O. cumana seeds would therefore act by mimicking the effects of an ABA treatment.
  • the miPEP319b of O. cumana shows only a very weak inhibitory effect on radicle protrusion at the lowest concentration of ( ⁇ )-GR24 or DCL coupled with the highest concentration of peptide.
  • this miPEP has no effect on metabolic recovery of seeds reflecting a lesser effect compared to miPEP319a.
  • P. ramosa miPEP319a induced similar effects to O. cumana with strong inhibition of germination following treatment with miPEP319a versus a weak effect for miPEP319b.
  • miR319a The involvement of miR319 in germination would thus be conserved between these two holoparasitic plants with different effects between members of the same family. miPEP319a can then disrupt the germination process of broomrapes by specifically reducing the expression of its associated miRNA target genes. Interestingly, in this study, it has been demonstrated that the GR24-dependent CYP707A1 expression can be down regulated by a treatment with miPEP319a, suggesting that the corresponding miR319 may interact with this signaling pathway in O. cumana.
  • CYP707A 1 is not a primary target of miR319a. This implies that miR319a could induce the mRNA degradation of at least one of the transcription factors TCP or MYB, leading to a modulation of the expression level of its target genes, and then finally to the down regulation of CYP707A 1. In the absence of effective functional genomics tools for root parasitic plants, the demonstration of this hypothesis remains unlikely.
  • TCP transcription factors of the TCP family are component of strigolactones signaling pathway in pea (PsBRCI), bread wheat (TaTB1), and Arabidopsis (AtBRCI and AtTCPI) for other physiological processes than germination (Braun et al., 2012, Plant Physiology 158(1): 225-238; Liu et al., 2017, Plant Physiology 174(3): 1931- 1948; Wang et al., 2020, Nature 583(7815): 277-281). It is therefore plausible to propose that a TCP transcription factor targeted by miR319a is a positive actor in the germination signaling pathway of O. cumana, which could explain the inhibitory effect of miPEP319a on this process.
  • miPEPs peptides
  • product development strategies could include sunflower coated seeds with miPEPs increasing sunflower’s resistance against broomrape or freeze- dried miPEPs inhibiting broomrape growth that could be diluted and directly sprayed in fields.

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Abstract

The present invention relates to the use of micropeptides (peptides encoded by microRNAs or "miPEPs") for inhibiting the growth of parasitic plants, in particular that of broomrapes. The present invention also relates to the compositions and methods for inhibiting the growth of parasitic plants, in particular that of broomrapes.

Description

DESCRIPTION
TITLE: MICROPEPTIDES AND USE OF THE SAME FOR INHIBITING THE GROWTH OF PARASITIC PLANTS
FIELD OF THE INVENTION
The present invention relates to the use of micropeptides (peptides encoded by microRNAs or "miPEPs") for inhibiting the growth of parasitic plants, in particular that of broomrapes. The present invention also relates to the compositions and methods for inhibiting the growth of parasitic plants, in particular that of broomrapes.
BACKGROUNG
Broomrapes, Orobanche and Phelipanche species, represent a major group of achlorophyllous root parasitic plants from the Orobanchaceae family which entirely depend on their hosts for water, mineral, and carbohydrate supplies (Delavault, 2015, Helia 38(62): 15-29). As a result of this parasitism, broomrapes count for the most destructive pests of important crop such as sunflower, oilseed rape, tomato and tobacco around the Mediterranean Sea and in Europe, and their impact significantly rose in the last few years (). Due to the small size of the seeds and the high amount produced per plant, they are endowed with exceptional dissemination abilities and with an extreme adaptation capacity. This results in a propagation that is almost uncontrollable (Parker, 2012, Weed Science 60(2): 269-276). Among those parasitic weeds, Orobanche cumana, the sunflower broomrape, is the most serious biotic constraint to sunflower in countries where it is cultivated (Euro-Asia and North Africa) with the exception of North and South America (Molinero-Ruiz et al. , 2015, Spanish Journal of Agricultural Research 13(4): e10R01). Yield reductions due to the infection by O. cumana frequently reach 100% in heavily infested fields (Shi & Zhao, 2020, Oilseeds & fats Crops and Lipids 27: 30).
Several control strategies have been employed against parasitic plants, but none of them have led to unequivocal success (Perez-De-Luque et al., 2009, Weed Research 49(s1): 8-22). Currently, the only efficient way of preventing root parasitic plant expansion is the selection for crop resistance. However, resistance to broomrapes is scarce and complex, making resistance breeding difficult (Molinero-Ruiz et al., 2015, Spanish Journal of Agricultural Research 13(4): e10R01). This approach has been mainly employed in sunflower against O. cumana, but to avoid the emergence of new pathogenic races overcoming the prevailing resistance genes, the production of new resistant lines cumulating several resistance traits associated with an integrative pest management approach is strongly recommended. However, this requires a better knowledge of the parasite but few aspects of the biology of broomrapes have been described. In conclusion, despite intense efforts to develop effective agricultural practices, selective control of various parasitic plants is still scarce and ineffective in terms of sustainability.
IN BRIEF
To overcome this agronomic issue and provide a control strategy with unequivocal success, the inventors have developed novel, effective and environmentally friendly micropeptides (peptides encoded by microRNAs or "miPEPs") with which to effectively inhibit the growth of parasitic plants will no longer be a problem. A first subject matter of the invention is thus the use of said miPEPs for inhibiting the growth of parasitic plants, in particular that of broomrapes. In parallel, a second subject matter of the invention relates to method for inhibiting the growth of parasitic plants, in particular that of broomrapes. Another subject matter of the invention also relates to composition comprising said miPEPs.
DETAILED DESCRIPTION
As a preamble, the invention relates to the subject matter as defined below and unless otherwise stated or the context otherwise requires, all terms have their ordinary meaning in the relevant field(s).
In general, a first aspect of the invention relates to a use of a miPEP for inhibiting the growth of a parasitic plant, wherein said miPEP regulates the expression of a miR encoded by the pri- miR, the miPEP of which comes from, said miR and pri-miR being expressed by the parasitic plant.
By “miRs”, it means microRNAs or miRNAs which are small non-coding RNAs (ribonucleic acids), about 21 nucleotides in length after maturation, which control expression of target genes at the post-transcriptional level, by degrading the target mRNA or by inhibiting its translation. The miRNAs occur in plants and animals. The target genes are often key genes in developmental processes. For example, they encode transcription factors or proteins of the proteasome. The regulation of expression of the miRs is poorly understood, but it is known in particular that the latter involves, like most coding genes, an RNA polymerase II: this enzyme produces a primary transcript, called “pri-miR”, which is then matured by a protein complex in particular containing the Dicer type enzymes. This maturation leads firstly to the formation of a precursor of miR called “pre-miR”, having a stem-loop secondary structure containing the miR and its complementary sequence miR*. Then the precursor is matured, which leads to formation of a shorter double-stranded RNA containing the miR and the miR*. The miR is then manipulated by the RISC complex, which cleaves the messenger RNA (mRNA) of the target gene or inhibits its translation.
By “miPEPs”, it means miR-encoded peptides which are a class of small peptides found to be encoded by pri-miRs through particular open reading frame (or ORF) called specifically miORFs, and that are capable of regulating the expression of a miR encoded by the pri-miR the miPEP of which comes from. The impact of a miPEP on the expression of its miR may result in the inhibition of the genes targeted by said miR, however, does not predict its inhibitory effect on the phenotype. The miPEPs in the sense of the present invention must not be understood as necessarily being peptides of small size, as “micro” does not refer to the size of the peptide.
Although this is fully accepted in the scientific community, it is reminded that the terms "miR" and "miPEP" may be preceded and/or followed by abbreviations that specifically designate a miR and/or a miPEP and differentiate them from one another. Classically, the prefix designates the genus and the species, and the suffix designates the gene from which said miR and/or said miPEP originate. For example, in the context of peptides described herein:
■ OcmiR and OcmiPEP, for example, refer to a miR and a miPEP, respectively, derived from the plant Orobanche cumana (Oc);
■ miR390 and miPEP390, for example, refer to a miR and a miPEP, respectively, of the miR390 gene; and
■ OcmiR390 and OcmiPEP390, for example, refer to a miR and a miPEP, respectively, derived from the miR390 gene of the plant Orobanche cumana (Oc).
The existence of different prefixes and/or suffixes makes it thus possible to clearly designate molecules that are distinct from one another.
By “parasitic plants”, it means plants that derive some or all of its nutritional requirements from another living plants. It has to be thus understood that parasitic plants are not usual plants and that the knowledge that exists on plants in the common sense cannot be transposed to them mutatis mutandis. In the context of the invention, parasitic plants particularly belong to Orobanchaceae family and preferably belongs to Orobanche and Phelipanche species among which are Orobanche cumana (Oc) and Phelipanche ramosa (Pr) respectively. By “inhibiting the growth of a parasitic plant”, it means that when an isolated or a chemically- synthetised miPEP is applied to the parasitic plant, it efficiently avoids the parasitic plant to grow. At the biomolecular level, the miPEP positively and specifically activates the transcription of its encoding pri-miRNA. As a result, it indirectly increases the level of its mature miR and induces the downregulation of the expression of the corresponding target genes involved in the growth of said parasitic plant. Consequently, if no molecular processes come to compensate or cancel the impact of said miPEP, the parasitic plant cannot grow or even dies. For this purpose, it is possible to use miPEPs capable of targeting either seed germination or seedling haustoriogenesis. According to another embodiment, the subject matter of the invention thus concerns the above-described use of a miPEP, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant; both germination and haustoriogenesis being induced by specific biochemical molecules exuded by the roots of the host plant detected by the parasitic plant. Consequently, it has to be understood that the subject matter of the invention concerns as much the above-described use of a miPEP, wherein:
- said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seed germination of said parasitic plant; or
- said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seedling haustoriogenesis of said parasitic plant; or
- said inhibition of the growth of said parasitic plant is obtained by the inhibition of the seed germination of said parasitic plant and the inhibition of the seedling haustoriogenesis of said parasitic plant.
Interestingly, while the parasitic plant needs the host plant to germinate and develop (see above), the host plant is not involved in eliminating the parasitic plant. Indeed, the genetic specificity of the miPEPs of the Invention makes it possible to target only the parasitic plant without impacting the host plant. This is all the more true when considering the inhibition of the seedling haustoriogenesis of the parasitic plant since this mechanism is specific to parasitic plants, host plants lacking it.
By “inhibition of the germination”, it means that when an isolated or a chemically-synthetised miPEP is applied to the parasitic plant, the development of a new individual from a seed placed in favourable conditions is prevented. For this purpose, two different mechanisms may be targeted:
- the seed radicle protrusion; and - the seed metabolic recovery.
According to another embodiment, the subject matter of the invention thus concerns the abovedescribed use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant. Consequently, it has to be understood that the subject matter of the invention concerns as well the above-described use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant; as the above-described use of a miPEP, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and by the inhibition of the metabolic recovery of said parasitic plant.
By “inhibition of the haustoriogenesis”, it means that when an isolated or a chemically- synthetised miPEP is applied to the parasitic plant, the development of a haustorium which is a rootlike structure that grows into or around another structure to absorb water or nutrients is prevented.
In general, a second aspect of the invention relates to a method for inhibiting the growth of a parasitic plant comprising a step of externally (exogenously) introducing a miPEP into said parasitic plant, wherein said miPEP regulates the expression of miR encoded by a pri-miR, the miPEP of which comes from.
By “a step of externally (exogenously) introducing a miPEP into said parasitic plant”, it means that said miPEP is artificially introduced by external means (watering, spraying, adding a compost, etc.) into the parasitic plant at any stage of its development. In particular, the implementation of this step is made when the parasitic plant is in the seed stage or starting to germinate, so as to potentiate the action of the miPEP. According to another embodiment, the subject matter of the invention thus concerns the above-described method, wherein said miPEP is externally introduced into said parasitic plant:
- by watering;
- by spraying; or
- by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
With regard to the latter means of implementing the method of the invention, it is recalled here that parasitic plants live on the hooks of host plants which they. Therefore, it is possible to take advantage, for example, of the addition of a fertilizer to promote the development of host plants (e.g. sunflower) by incorporating at a certain dose in this fertilizer the miPEPs of the Invention so as to prevent the development of parasitic plants (O. cumana) at the same time as the growth of the host plants is promoted.
Talking about the dose, another subject of the invention concerns the above-described method, wherein said miPEP is externally introduced into said parasitic plant in the form of a liquid (watering, spraying) or solid (fertilizer, compost, culture substrate) composition comprising from 10'9 M to 10'1 M (or from 10'9 M to 10'4 M, or from 10'4 M to 10'1 M) of said miPEP or comprising, in particular, 10'9 M, 10'8 M, 10'7 M, 10'6 M, 10'5 M, 10'4 M, 10'3 M, 10'2 M or 10'1 M of said miPEP.
Complementary to these first two aspects, another general subject matter of the invention relates to a composition comprising from 10'9 M to 10'1 M of a miPEP or comprising, in particular, 10’9 M, 10’8 M, 10’7 M, 10’6 M, 10’5 M, 10’4 M, 10’3 M, 10’2 M or 10’1 M of a miPEP. On this point, it should be noted that the composition of the invention does not exist in the natural state, and this is all the more true as such a concentration of miPEP cannot exist within a parasitic plant cell.
It should be also noted that “composition comprising a miPEP” means that the composition of the invention comprises at least one miPEP. Consequently, a mixture of miPEPs is conceivable, said miPEPs being able to target a single miR or several miRs depending on the pri-miR(s) from which they are coming from. In this respect, the above-mentioned concentrations refer either to the mixture of miPEPs as such, or to each of the miPEPs of said mixture, said miPEPs being able to be at the same concentration or being able to be at different concentrations among those mentioned above.
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition, said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. Thus, the composition of the invention may be a phytopharmaceutical composition, an herbicide composition or a coating composition.
For example, a solvent may be chosen among: acetone, acetonitrile, acetic acid, formic acid, dimethyl adipate, benzyl acetate, bi-butyl carbonate, dimethyl sulfoxide (DMSO), water, dimethyl glutarate, ammonium hydroxide, iso-butanol, iso-propanol, diethylhexyl lactate, light aromatic naphtha, heavy aromatic naphtha, diethyl succinate and mixtures thereof (e.g., mixture [water; acetic acid]; [acetonitrile; acetic acid], [water, acetonitrile; acetic acid], [water; DMSO], [water; acetonitrile] or [water; ammonium hydroxide]). Among the mixtures mentioned, the latter may in particular be an organic solvent mixture [acetonitrile; acetic acid] in a volume:volume ratio of 99:1 to 1 :99, a mixture [water; DMSO] in a volume:volume ratio of 99:1 to 1 :99, a mixture [water; acetonitrile] in a volume:volume ratio of 99:1 to 1 :99 or a mixture [water; ammonium hydroxide] in a volume:volume ratio of 99:1 to 99.9:0.1. The miPEPs can also be solubilised in a solution comprising 50% acetonitrile, 10% acetic acid and 40% water (v/v/v).
For example, a fixing agent may be chosen among: carboxymethyl cellulose (CMC), gum Arabic. As fixing agents are chemical or natural agents which makes it possible to stick the composition of the invention to a plant seed so as to coat the said plant seed, for instance, to apply and hold the active substance(s) (i.e. miPEPs) on the seed, it is understood that one embodiment of invention relates to a coating composition comprising a miPEP. It is therefore understood that another aspect of the invention relates to a coated seed comprising a host plant seed (e.g. sunflower), said host plant seed being coated with the above-described coating composition comprising a miPEP able to inhibit the growth of a parasitic plant (e.g. O. cumana). The coating may be carried out according to the processes conventionally used in the agronomic/food industry and may be obtained by using a material capable of disintegrating in a solvent or in the soil, such as a binder or clay. According to the invention, the coating can be used to impart particular properties to a seed such as improved host plant growth or resistance to certain biotic or abiotic stresses.
In view of the above, and in the context of the invention, it has to be pointed out that the targeted miR by the miPEP of the invention is in particular chosen among: miR156a, miR156b, miR156c, miR156d, miR156e, miR156f, miR156g, miR159, miR160a, miR160b, miR160c, miR160d, miR160e, miR164, miR164a, miR164b, miR164c, miR166a, miR166b, miR166c, miR166d, miR166e, miR167a, miR167b, miR167c, miR168, miR169a, miR169b, miR171a, miR171 b, miR171c, miR172a, miR172b, miR172c, miR172d, miR172e, miR172f, miR172g, miR319a, miR319b, miR390, miR393a, miR393b, miR394, miR395, miR396a, miR396b and miR396c. Consequently, it has to be understood the invention encompasses many aspects and embodiments which are for some explicitly described hereafter.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a. Surprisingly and unexpectedly, the inventors found that the use of miPEP319a specifically directed against miR319a expressed by parasitic plants, was able to inhibit the growth of both O. cumana and P. ramosa. In other words, with this tool, the inventors have developed a universal and easy- to-use tool against these parasitic plants.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said parasitic plant belongs to Orobanchaceae family.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346. By “percentage of identity”, it means the percentage determined by direct comparison of two oligonucleotide (or amino acid) sequences by determining the number of identical nucleotides (or residues) between the two sequences, dividing by the number of nucleotides (or residues) in the longer of the two sequences and multiplying by 100. The expression “at least 80%” also means that the aforementioned percentage identity is at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100%. In this respect, it should be noted that this definition applies to all embodiments of the invention.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant:
- by watering;
- by spraying; or
- by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said parasitic plant belongs to Orobanchaceae family.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346.
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 1 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 3 or 4 (present on the pre-miR156a of sequence SEQ ID NO: 2).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6 (encoded by the miORF156a of sequence SEQ ID NO: 5).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156a into said parasitic plant, wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 1 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 3 or 4 (present on the pre-miR156a of sequence SEQ ID NO: 2).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6 (encoded by the miORF156a of sequence SEQ ID NO: 5). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 6, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 7, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 9 or 10 (present on the pre-miR156b of sequence SEQ ID NO: 8).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 12 (encoded by the miORF156b of sequence SEQ ID NO: 11). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156b into said parasitic plant, wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 7, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 9 or 10 (present on the pre-miR156b of sequence SEQ ID NO: 8).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 12 (encoded by the miORF156b of sequence SEQ ID NO: 11).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 12, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 13, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 15 or 16 (present on the pre-miR156c of sequence SEQ ID NO: 14).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said miPEP156c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 18 (encoded by the miORF156c of sequence SEQ ID NO: 17).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156c into said parasitic plant, wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 13, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 15 or 16 (present on the pre-miR156c of sequence SEQ ID NO: 14).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 18 (encoded by the miORF156c of sequence SEQ ID NO: 17).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 18, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 19, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 21 or 22 (present on the pre-miR156d of sequence SEQ ID NO: 20).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 24 (encoded by the miORF156d of sequence SEQ ID NO: 23).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156d into said parasitic plant, wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 19, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 21 or 22 (present on the pre-miR156d of sequence SEQ ID NO: 20).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 24 (encoded by the miORF156d of sequence SEQ ID NO: 23).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 24, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 25, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 27 or 28 (present on the pre-miR156e of sequence SEQ ID NO: 26).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 30 (encoded by the miORF156e of sequence SEQ ID NO: 29).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156e into said parasitic plant, wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 25, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 27 or 28 (present on the pre-miR156e of sequence SEQ ID NO: 26).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 30 (encoded by the miORF156e of sequence SEQ ID NO: 29). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 30, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156f for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP156f regulates the expression of miR156f encoded by a pri-miR156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEPI 56f, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEPI 56f, wherein said pri-miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 31 , in particular wherein said miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 33 or 34 (present on the pre-miR156f of sequence SEQ ID NO: 32).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said miPEP156f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 36 (encoded by the miORF156f of sequence SEQ ID NO: 35). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP156f into said parasitic plant, wherein said miPEP156f regulates the expression of miR156f encoded by a pri-miR156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 31 , in particular wherein said miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 33 or 34 (present on the pre-miR156f of sequence SEQ ID NO: 32).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 36 (encoded by the miORF156f of sequence SEQ ID NO: 35).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156f having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 36, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP159 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP159 regulates the expression of miR159 encoded by a pri-miR159.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP159, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEPI 59, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEPI 59, wherein said pri-miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 37, in particular wherein said miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 39 or 40 (present on the pre-miR159 of sequence SEQ ID NO: 38).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEPI 59, wherein said miPEPI 59 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 42 (encoded by the miORF159 of sequence SEQ ID NO: 41).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEPI 59 into said parasitic plant, wherein said miPEPI 59 regulates the expression of miR159 encoded by a pri- miR159.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEPI 59 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP159 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP159.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 37, in particular wherein said miR159 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 39 or 40 (present on the pre-miR159 of sequence SEQ ID NO: 38).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP159 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 42 (encoded by the miORF159 of sequence SEQ ID NO: 41).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP159 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 42, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP160a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160a regulates the expression of miR160a encoded by a pri-miR160a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP160a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160a, wherein said pri-miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 43, in particular wherein said miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 45 or 46 (present on the pre-miR160a of sequence SEQ ID NO: 44).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160a, wherein said miPEP160a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48 (encoded by the miQRF160a of sequence SEQ ID NO: 47).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160a into said parasitic plant, wherein said miPEP160a regulates the expression of miR160a encoded by a pri-miR160a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP160a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 43, in particular wherein said miR160a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 45 or 46 (present on the pre-miR160a of sequence SEQ ID NO: 44).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48 (encoded by the miQRF160a of sequence SEQ ID NO: 47).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP160a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 48, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP160b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160b regulates the expression of miR160b encoded by a pri-miR160b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP160b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said pri-miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 49, in particular wherein said miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 51 or 52 (present on the pre-miR160b of sequence SEQ ID NO: 50).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160b, wherein said miPEP160b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54 (encoded by the miQRF160b of sequence SEQ ID NO: 53).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160b into said parasitic plant, wherein said miPEP160b regulates the expression of miR160b encoded by a pri-miR160b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP160b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 49, in particular wherein said miR160b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 51 or 52 (present on the pre-miR160b of sequence SEQ ID NO: 50).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54 (encoded by the miQRF160b of sequence SEQ ID NO: 53). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP160b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 54, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP160c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160c regulates the expression of miR160c encoded by a pri-miR160c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP160c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said pri-miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 55, in particular wherein said miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 57 or 58 (present on the pre-miR160c of sequence SEQ ID NO: 56).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160c, wherein said miPEP160c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59. According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160c into said parasitic plant, wherein said miPEP160c regulates the expression of miR160c encoded by a pri-miR160c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP160c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 55, in particular wherein said miR160c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 57 or 58 (present on the pre-miR160c of sequence SEQ ID NO: 56).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59.
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP160c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 59, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP160d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160d regulates the expression of miR160d encoded by a pri-miR160d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP160d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said pri-miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 60, in particular wherein said miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 62 or 63 (present on the pre-miR160d of sequence SEQ ID NO: 61).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160d, wherein said miPEP160d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65 (encoded by the miQRF160d of sequence SEQ ID NO: 64).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160d into said parasitic plant, wherein said miPEP160d regulates the expression of miR160d encoded by a pri-miR160d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP160d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 60, in particular wherein said miR160d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 62 or 63 (present on the pre-miR160d of sequence SEQ ID NO: 61).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65 (encoded by the miQRF160d of sequence SEQ ID NO: 64).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP160d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 65, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP160e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP160e regulates the expression of miR160e encoded by a pri-miR160e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP160e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said pri-miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 66, in particular wherein said miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 68 or 69 (present on the pre-miR160e of sequence SEQ ID NO: 67).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP160e, wherein said miPEP160e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 (encoded by the miQRF160e of sequence SEQ ID NO: 70).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP160e into said parasitic plant, wherein said miPEP160e regulates the expression of miR160e encoded by a pri-miR160e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP160e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 66, in particular wherein said miR160e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 68 or 69 (present on the pre-miR160e of sequence SEQ ID NO: 67).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP160e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 (encoded by the miQRF160e of sequence SEQ ID NO: 70).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP160e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 71 , in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP164 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP164 regulates the expression of miR164 encoded by a pri-miR164.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP164, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164, wherein said pri-miR164 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 72, in particular wherein said miR164 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 74 or 75 (present on the pre-miR164 of sequence SEQ ID NO: 73).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164, wherein said miPEP164 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 76.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP164 into said parasitic plant, wherein said miPEP164 regulates the expression of miR164 encoded by a pri- miR164.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP164.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 72, in particular wherein said miR164 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 74 or 75 (present on the pre-miR164 of sequence SEQ ID NO: 73).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 76. According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP164 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 76, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP166a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166a regulates the expression of miR166a encoded by a pri-miR166a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP166a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166a, wherein said pri-miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 77, in particular wherein said miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 79 or 80 (present on the pre-miR166a of sequence SEQ ID NO: 78).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166a, wherein said miPEP166a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 82 (encoded by the miORF166a of sequence SEQ ID NO: 81). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166a into said parasitic plant, wherein said miPEP166a regulates the expression of miR166a encoded by a pri-miR166a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP166a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 77, in particular wherein said miR166a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 79 or 80 (present on the pre-miR166a of sequence SEQ ID NO: 78).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 82 (encoded by the miORF166a of sequence SEQ ID NO: 81).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP166a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 82, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP166b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166b regulates the expression of miR166b encoded by a pri-miR166b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP166b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said pri-miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 83, in particular wherein said miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 85 or 86 (present on the pre-miR166b of sequence SEQ ID NO: 84).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166b, wherein said miPEP166b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 88 (encoded by the miORF166b of sequence SEQ ID NO: 87).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166b into said parasitic plant, wherein said miPEP166b regulates the expression of miR166b encoded by a pri-miR166b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP166b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 83, in particular wherein said miR166b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 85 or 86 (present on the pre-miR166b of sequence SEQ ID NO: 84).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 88 (encoded by the miORF166b of sequence SEQ ID NO: 87).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP166b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 88, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP166c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166c regulates the expression of miR166c encoded by a pri-miR166c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP166c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166c, wherein said pri-miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 89, in particular wherein said miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 91 or 92 (present on the pre-miR166c of sequence SEQ ID NO: 90).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166c, wherein said miPEP166c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 94 (encoded by the miORF166c of sequence SEQ ID NO: 93).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166c into said parasitic plant, wherein said miPEP166c regulates the expression of miR166c encoded by a pri-miR166c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP166c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 89, in particular wherein said miR166c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 91 or 92 (present on the pre-miR166c of sequence SEQ ID NO: 90).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 94 (encoded by the miORF166c of sequence SEQ ID NO: 93).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP166c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 94, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP166d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166d regulates the expression of miR166d encoded by a pri-miR166d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP166d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said pri-miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 95, in particular wherein said miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 97 or 98 (present on the pre-miR166d of sequence SEQ ID NO: 96).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166d, wherein said miPEP166d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100 (encoded by the miORF166d of sequence SEQ ID NO: 99).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166d into said parasitic plant, wherein said miPEP166d regulates the expression of miR166d encoded by a pri-miR166d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP166d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 95, in particular wherein said miR166d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 97 or 98 (present on the pre-miR166d of sequence SEQ ID NO: 96).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100 (encoded by the miORF166d of sequence SEQ ID NO: 99). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP166d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 100, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP166e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP166e regulates the expression of miR166e encoded by a pri-miR166e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP166e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said pri-miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 101 , in particular wherein said miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 103 or 104 (present on the pre-miR166e of sequence SEQ ID NO: 102).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP166e, wherein said miPEP166e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106 (encoded by the miORF166e of sequence SEQ ID NO: 105). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP166e into said parasitic plant, wherein said miPEP166e regulates the expression of miR166e encoded by a pri-miR166e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP166e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 101 , in particular wherein said miR166e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 103 or 104 (present on the pre-miR166e of sequence SEQ ID NO: 102).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP166e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106 (encoded by the miORF166e of sequence SEQ ID NO: 105).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP166e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 106, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP167a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167a regulates the expression of miR167a encoded by a pri-miR167a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP167a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said pri-miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 107, in particular wherein said miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 109 or 110 (present on the pre-miR167a of sequence SEQ ID NO: 108).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167a, wherein said miPEP167a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112 (encoded by the miORF167a of sequence SEQ ID NO: 111).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167a into said parasitic plant, wherein said miPEP167a regulates the expression of miR167a encoded by a pri-miR167a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP167a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 107, in particular wherein said miR167a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 109 or 110 (present on the pre-miR167a of sequence SEQ ID NO: 108).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112 (encoded by the miORF167a of sequence SEQ ID NO: 111).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP167a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 112, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP167b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167b regulates the expression of miR167b encoded by a pri-miR167b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP167b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said pri-miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 113, in particular wherein said miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 115 or 116 (present on the pre-miR167b of sequence SEQ ID NO: 114).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167b, wherein said miPEP167b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118 (encoded by the miORF167b of sequence SEQ ID NO: 117).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167b into said parasitic plant, wherein said miPEP167b regulates the expression of miR167b encoded by a pri-miR167b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP167b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 113, in particular wherein said miR167b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 115 or 116 (present on the pre-miR167b of sequence SEQ ID NO: 114).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118 (encoded by the miORF167b of sequence SEQ ID NO: 117).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP167b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 118, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP167c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP167c regulates the expression of miR167c encoded by a pri-miR167c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP167c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said pri-miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 119, in particular wherein said miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 121 or 122 (present on the pre-miR167c of sequence SEQ ID NO: 120).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP167c, wherein said miPEP167c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 124 (encoded by the miORF167c of sequence SEQ ID NO: 123).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP167c into said parasitic plant, wherein said miPEP167c regulates the expression of miR167c encoded by a pri-miR167c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP167c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 119, in particular wherein said miR167c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 121 or 122 (present on the pre-miR167c of sequence SEQ ID NO: 120).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP167c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 124 (encoded by the miORF167c of sequence SEQ ID NO: 123). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP167c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 124, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP168 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP168 regulates the expression of miR168 encoded by a pri-miR168.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP168, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said pri-miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 125, in particular wherein said miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 127 or 128 (present on the pre-miR168 of sequence SEQ ID NO: 126).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP168, wherein said miPEP168 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130 (encoded by the miORF168 of sequence SEQ ID NO: 129). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP168 into said parasitic plant, wherein said miPEP168 regulates the expression of miR168 encoded by a pri- miR168.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP168.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 125, in particular wherein said miR168 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 127 or 128 (present on the pre-miR168 of sequence SEQ ID NO: 126).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP168 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130 (encoded by the miORF168 of sequence SEQ ID NO: 129).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP168 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 130, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP169a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP169a regulates the expression of miR169a encoded by a pri-miR169a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP169a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said pri-miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 131 , in particular wherein said miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 133 or 134 (present on the pre-miR169a of sequence SEQ ID NO: 132).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169a, wherein said miPEP169a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136 (encoded by the miORF169a of sequence SEQ ID NO: 135).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP169a into said parasitic plant, wherein said miPEP169a regulates the expression of miR169a encoded by a pri-miR169a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP169a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 131 , in particular wherein said miR169a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 133 or 134 (present on the pre-miR169a of sequence SEQ ID NO: 132).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136 (encoded by the miORF169a of sequence SEQ ID NO: 135).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP169a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 136, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP169b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP169b regulates the expression of miR169b encoded by a pri-miR169b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP169b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said pri-miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 137, in particular wherein said miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 139 or 140 (present on the pre-miR169b of sequence SEQ ID NO: 138).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP169b, wherein said miPEP169b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142 (encoded by the miORF169b of sequence SEQ ID NO: 141).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP169b into said parasitic plant, wherein said miPEP169b regulates the expression of miR169b encoded by a pri-miR169b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP169b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 137, in particular wherein said miR169b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 139 or 140 (present on the pre-miR169b of sequence SEQ ID NO: 138).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP169b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142 (encoded by the miORF169b of sequence SEQ ID NO: 141).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP169b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 142, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP171a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171a regulates the expression of miR171a encoded by a pri-miR171a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP171a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171a, wherein said pri-miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 143, in particular wherein said miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 145 or 146 (present on the pre-miR171a of sequence SEQ ID NO: 144).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171a, wherein said miPEP171a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 148 (encoded by the miORF171a of sequence SEQ ID NO: 147).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP171a into said parasitic plant, wherein said miPEP171a regulates the expression of miR171a encoded by a pri-miR171a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP171a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 143, in particular wherein said miR171a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 145 or 146 (present on the pre-miR171a of sequence SEQ ID NO: 144).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 148 (encoded by the miORF171a of sequence SEQ ID NO: 147). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP171a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 148, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP171 b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171 b regulates the expression of miR171 b encoded by a pri-miR171 b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171 b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP171 b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171 b, wherein said pri-miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 149, in particular wherein said miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 151 or 152 (present on the pre-miR171 b of sequence SEQ ID NO: 150).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171 b, wherein said miPEP171b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154 (encoded by the miORF171b of sequence SEQ ID NO: 153). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP171 b into said parasitic plant, wherein said miPEP171b regulates the expression of miR171 b encoded by a pri-miR171b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171 b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP171b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 149, in particular wherein said miR171b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 151 or 152 (present on the pre-miR171 b of sequence SEQ ID NO: 150).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154 (encoded by the miORF171b of sequence SEQ ID NO: 153).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP171b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 154, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP171c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP171c regulates the expression of miR171c encoded by a pri-miR171c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP171c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said pri-miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 155, in particular wherein said miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 157 or 158 (present on the pre-miR171c of sequence SEQ ID NO: 156).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP171c, wherein said miPEP171c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160 (encoded by the miORF171c of sequence SEQ ID NO: 159).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP171c into said parasitic plant, wherein said miPEP171c regulates the expression of miR171c encoded by a pri-miR171c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP171c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 155, in particular wherein said miR171c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 157 or 158 (present on the pre-miR171c of sequence SEQ ID NO: 156).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP171c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160 (encoded by the miORF171c of sequence SEQ ID NO: 159).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP171c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 160, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 161 , in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 163 or 164 (present on the pre-miR172a of sequence SEQ ID NO: 162).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said miPEP172a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 166 (encoded by the miORF172a of sequence SEQ ID NO: 165).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172a into said parasitic plant, wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 161 , in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 163 or 164 (present on the pre-miR172a of sequence SEQ ID NO: 162).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 166 (encoded by the miORF172a of sequence SEQ ID NO: 165).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 166, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172e for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 167, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 169 or 170 (present on the pre-miR172e of sequence SEQ ID NO: 168).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172 (encoded by the miORF172e of sequence SEQ ID NO: 171).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172e into said parasitic plant, wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 167, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 169 or 170 (present on the pre-miR172e of sequence SEQ ID NO: 168).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172 (encoded by the miORF172e of sequence SEQ ID NO: 171). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 172, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 173, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 175 or 176 (present on the pre-miR172b of sequence SEQ ID NO: 174).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 178 (encoded by the miORF172b of sequence SEQ ID NO: 177). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172b into said parasitic plant, wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 173, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 175 or 176 (present on the pre-miR172b of sequence SEQ ID NO: 174).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 178 (encoded by the miORF172b of sequence SEQ ID NO: 177).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 178, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 179, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 181 or 182 (present on the pre-miR172c of sequence SEQ ID NO: 180).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 184 (encoded by the miORF172c of sequence SEQ ID NO: 183).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172c into said parasitic plant, wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 179, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 181 or 182 (present on the pre-miR172c of sequence SEQ ID NO: 180).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 184 (encoded by the miORF172c of sequence SEQ ID NO: 183).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 184, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172d for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 185, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 187 or 188 (present on the pre-miR172d of sequence SEQ ID NO: 186).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190 (encoded by the miORF172d of sequence SEQ ID NO: 189).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP172d into said parasitic plant, wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 185, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 187 or 188 (present on the pre-miR172d of sequence SEQ ID NO: 186).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190 (encoded by the miORF172d of sequence SEQ ID NO: 189).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 190, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 191 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193 or 194 (present on the pre-miR319a of sequence SEQ ID NO: 192).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196 (encoded by the miORF319a of sequence SEQ ID NO: 195).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 191 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193 or 194 (present on the pre-miR319a of sequence SEQ ID NO: 192).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196 (encoded by the miORF319a of sequence SEQ ID NO: 195). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 196, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP319b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP319b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 197, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 199 or 200 (present on the pre-miR319b of sequence SEQ ID NO: 198).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202 (encoded by the miORF319b of sequence SEQ ID NO: 201). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP319b into said parasitic plant, wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 197, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 199 or 200 (present on the pre-miR319b of sequence SEQ ID NO: 198).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202 (encoded by the miORF319b of sequence SEQ ID NO: 201).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP319b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 202, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP390 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP390 regulates the expression of miR390 encoded by a pri-miR390.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP390, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said pri-miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 203, in particular wherein said miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 205 or 206 (present on the pre-miR390 of sequence SEQ ID NO: 204).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP390, wherein said miPEP390 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208 (encoded by the miQRF390 of sequence SEQ ID NO: 207).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP390 into said parasitic plant, wherein said miPEP390 regulates the expression of miR390 encoded by a pri- miR390.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP390.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 203, in particular wherein said miR390 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 205 or 206 (present on the pre-miR390 of sequence SEQ ID NO: 204).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP390 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208 (encoded by the miQRF390 of sequence SEQ ID NO: 207).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP390 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 208, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP393a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP393a regulates the expression of miR393a encoded by a pri-miR393a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP393a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said pri-miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 209, in particular wherein said miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 211 or 212 (present on the pre-miR393a of sequence SEQ ID NO: 210).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393a, wherein said miPEP393a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214 (encoded by the miORF393a of sequence SEQ ID NO: 213).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP393a into said parasitic plant, wherein said miPEP393a regulates the expression of miR393a encoded by a pri-miR393a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP393a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 209, in particular wherein said miR393a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 211 or 212 (present on the pre-miR393a of sequence SEQ ID NO: 210).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214 (encoded by the miORF393a of sequence SEQ ID NO: 213).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP393a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 214, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP393b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP393b regulates the expression of miR393b encoded by a pri-miR393b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP393b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said pri-miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 215, in particular wherein said miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 217 or 218 (present on the pre-miR393b of sequence SEQ ID NO: 216).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP393b, wherein said miPEP393b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220 (encoded by the miORF393b of sequence SEQ ID NO: 219).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP393b into said parasitic plant, wherein said miPEP393b regulates the expression of miR393b encoded by a pri-miR393b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP393b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 215, in particular wherein said miR393b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 217 or 218 (present on the pre-miR393b of sequence SEQ ID NO: 216).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP393b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220 (encoded by the miORF393b of sequence SEQ ID NO: 219). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP393b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 220, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP394 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP394 regulates the expression of miR394 encoded by a pri-miR394.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP394, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP394, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP394, wherein said pri-miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 221 , in particular wherein said miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 223 or 224 (present on the pre-miR394 of sequence SEQ ID NO: 222).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP394, wherein said miPEP394 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226 (encoded by the miORF394 of sequence SEQ ID NO: 225). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP394 into said parasitic plant, wherein said miPEP394 regulates the expression of miR394 encoded by a pri- miR394.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP394 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP394 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP394.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 221 , in particular wherein said miR394 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 223 or 224 (present on the pre-miR394 of sequence SEQ ID NO: 222).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP394 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226 (encoded by the miORF394 of sequence SEQ ID NO: 225).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP394 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 226, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP395 for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP395 regulates the expression of miR395 encoded by a pri-miR395.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP395, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said pri-miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 227, in particular wherein said miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 229 or 230 (present on the pre-miR395 of sequence SEQ ID NO: 228).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP395, wherein said miPEP395 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232 (encoded by the miORF395 of sequence SEQ ID NO: 231).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP395 into said parasitic plant, wherein said miPEP395 regulates the expression of miR395 encoded by a pri- miR395.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP395.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 227, in particular wherein said miR395 has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 229 or 230 (present on the pre-miR395 of sequence SEQ ID NO: 228).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP395 has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232 (encoded by the miORF395 of sequence SEQ ID NO: 231).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP395 having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 232, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP396a for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP396a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 233, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 235 or 236 (present on the pre-miR396a of sequence SEQ ID NO: 234).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238 (encoded by the miORF396a of sequence SEQ ID NO: 237).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396a into said parasitic plant, wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 233, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 235 or 236 (present on the pre-miR396a of sequence SEQ ID NO: 234).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238 (encoded by the miORF396a of sequence SEQ ID NO: 237).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP396a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 238, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP396b for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP396b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 239, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 241 or 242 (present on the pre-miR396b of sequence SEQ ID NO: 240).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244 (encoded by the miORF396b of sequence SEQ ID NO: 243).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396b into said parasitic plant, wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 239, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 241 or 242 (present on the pre-miR396b of sequence SEQ ID NO: 240).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244 (encoded by the miORF396b of sequence SEQ ID NO: 243). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP396b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 244, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP396c for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana), wherein said miPEP396c regulates the expression of miR396c encoded by a pri-miR396c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP396c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said pri-miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 245, in particular wherein said miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 247 or 248 (present on the pre-miR396c of sequence SEQ ID NO: 246).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396c, wherein said miPEP396c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250 (encoded by the miORF396c of sequence SEQ ID NO: 249). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Orobanche species (in particular O. cumana) comprising a step of externally introducing a miPEP396c into said parasitic plant, wherein said miPEP396c regulates the expression of miR396c encoded by a pri-miR396c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP396c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 245, in particular wherein said miR396c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 247 or 248 (present on the pre-miR396c of sequence SEQ ID NO: 246).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250 (encoded by the miORF396c of sequence SEQ ID NO: 249).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP396c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 250, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent. In addition to these various singular aspects and/or singular embodiments described above, the invention includes their combination. In particular, the following uses are described:
- the use of a miPEP for inhibiting the metabolic recovery of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR156a, miR166d, miR319a, miR393b and miR396b;
- the use of a miPEP for inhibiting the radicle protrusion of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR164, miR395, miR156a, miR156b, miR156c, miR156d, miR156e, miR156f, miR156g, miR160a, miR160b, miR160c, miR160d, miR166d, miR167a, miR167b, miR169a, miR169b, miR171 b, miR171c, miR172a, miR172b, miR172c, miR172d, miR172e, miR172g, miR319a, miR319b, miR393a, miR393b, miR396a, miR396b and miR396c;
- the use of a miPEP for inhibiting the metabolic recovery and (or) the radicle protrusion of parasitic plant seeds belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR159, miR156a, miR166d, miR319a, miR393b and miR396b;
- the use of a miPEP for inhibiting the haustoriogenesis of parasitic plant seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR172c, miR172d, miR319a and miR319b;
- the use of a miPEP for inhibiting the metabolic recovery and (or) the haustoriogenesis of parasitic plant seed and (or) seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of miR319a;
- the use of a miPEP for inhibiting the radicle protrusion and (or) the haustoriogenesis of parasitic plant seed and (or) seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of a miR chosen among from: miR172c and miR319a; and
- the use of a miPEP for inhibiting the metabolic recovery, the radicle protrusion and the haustoriogenesis of parasitic plant seed and (or) seedlings belonging to Orobanche species (in particular O. cumana), wherein said miPEP regulates the expression of miR319a, which can be declined as are the different singular aspects and/or singular modes of realisation described above (/.e. use, method, composition). According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 251 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 253 or 254 (present on the pre-miR156a of sequence SEQ ID NO: 252).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156a, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 256 (encoded by the miORF156a of sequence SEQ ID NO: 255).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156a into said parasitic plant, wherein said miPEP156a regulates the expression of miR156a encoded by a pri-miR156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 251 , in particular wherein said miR156a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 253 or 254 (present on the pre-miR156a of sequence SEQ ID NO: 252).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 256 (encoded by the miORF156a of sequence SEQ ID NO: 255).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 256, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 257, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 259 (present on the pre-miR156b of sequence SEQ ID NO: 258).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156b, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 (encoded by the miORF156b of sequence SEQ ID NO: 260).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156b into said parasitic plant, wherein said miPEP156b regulates the expression of miR156b encoded by a pri-miR156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 257, in particular wherein said miR156b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 259 (present on the pre-miR156b of sequence SEQ ID NO: 258).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 (encoded by the miORF156b of sequence SEQ ID NO: 260).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 261 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 262, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 264 (present on the pre-miR156c of sequence SEQ ID NO: 263).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156c, wherein said miPEP156c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 266 (encoded by the miORF156c of sequence SEQ ID NO: 265).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156c into said parasitic plant, wherein said miPEP156c regulates the expression of miR156c encoded by a pri-miR156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 262, in particular wherein said miR156c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 264 (present on the pre-miR156c of sequence SEQ ID NO: 263).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 266 (encoded by the miORF156c of sequence SEQ ID NO: 265). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 266, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156d for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 267, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 269 (present on the pre-miR156d of sequence SEQ ID NO: 268).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156d, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 (encoded by the miORF156d of sequence SEQ ID NO: 270). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156d into said parasitic plant, wherein said miPEP156d regulates the expression of miR156d encoded by a pri-miR156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 267, in particular wherein said miR156d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 269 (present on the pre-miR156d of sequence SEQ ID NO: 268).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 (encoded by the miORF156d of sequence SEQ ID NO: 270).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 271 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156e for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 272, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 274 or 275 (present on the pre-miR156e of sequence SEQ ID NO: 273).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156e, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277 (encoded by the miORF156e of sequence SEQ ID NO: 276).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156e into said parasitic plant, wherein said miPEP156e regulates the expression of miR156e encoded by a pri-miR156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 272, in particular wherein said miR156e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 274 or 275 (present on the pre-miR156e of sequence SEQ ID NO: 273).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277 (encoded by the miORF156e of sequence SEQ ID NO: 276).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 277, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156f for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156f regulates the expression of miR156f encoded by a pri-miR156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEPI 56f, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said pri-miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 278, in particular wherein said miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 280 or 281 (present on the pre-miR156f of sequence SEQ ID NO: 279).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156f, wherein said miPEP156f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 283 (encoded by the miORF156f of sequence SEQ ID NO: 282).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156f into said parasitic plant, wherein said miPEP156f regulates the expression of miR156f encoded by a pri-miR156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 278, in particular wherein said miR156f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 280 or 281 (present on the pre-miR156f of sequence SEQ ID NO: 279).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 283 (encoded by the miORF156f of sequence SEQ ID NO: 282).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156f having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 283, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP156g for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP156g regulates the expression of miR156g encoded by a pri-miR156g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156g, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP156g, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156g, wherein said pri-miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 284, in particular wherein said miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 286 or 287 (present on the pre-miR156g of sequence SEQ ID NO: 285).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP156g, wherein said miPEP156g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 289 (encoded by the miORF156g of sequence SEQ ID NO: 288).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP156g into said parasitic plant, wherein said miPEP156g regulates the expression of miR156g encoded by a pri-miR156g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156g is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156g is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP156g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 284, in particular wherein said miR156g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 286 or 287 (present on the pre-miR156g of sequence SEQ ID NO: 285).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP156g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 289 (encoded by the miORF156g of sequence SEQ ID NO: 288). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP156g having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 289, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP164a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP164a regulates the expression of miR164a encoded by a pri-miR164a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP164a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164a, wherein said pri-miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 290, in particular wherein said miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 292 or 293 (present on the pre-miR164a of sequence SEQ ID NO: 291).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164a, wherein said miPEP164a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295 (encoded by the miORF164a of sequence SEQ ID NO: 294). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164a into said parasitic plant, wherein said miPEP164a regulates the expression of miR164a encoded by a pri-miR164a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP164a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 290, in particular wherein said miR164a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 292 or 293 (present on the pre-miR164a of sequence SEQ ID NO: 291).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295 (encoded by the miORF164a of sequence SEQ ID NO: 294).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP164a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 295, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP164b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP164b regulates the expression of miR164b encoded by a pri-miR164b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP164b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said pri-miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 296, in particular wherein said miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 298 or 299 (present on the pre-miR164b of sequence SEQ ID NO: 297).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164b, wherein said miPEP164b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 301 (encoded by the miORF164b of sequence SEQ ID NO: 300).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164b into said parasitic plant, wherein said miPEP164b regulates the expression of miR164b encoded by a pri-miR164b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP164b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 296, in particular wherein said miR164b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 298 or 299 (present on the pre-miR164b of sequence SEQ ID NO: 297).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 301 (encoded by the miORF164b of sequence SEQ ID NO: 300).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP164b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 301 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP164c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP164c regulates the expression of miR164c encoded by a pri-miR164c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP164c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said pri-miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 302, in particular wherein said miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 304 (present on the pre-miR164c of sequence SEQ ID NO: 303).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP164c, wherein said miPEP164c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 306 (encoded by the miORF164c of sequence SEQ ID NO: 305).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP164c into said parasitic plant, wherein said miPEP164c regulates the expression of miR164c encoded by a pri-miR164c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP164c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 302, in particular wherein said miR164c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 304 (present on the pre-miR164c of sequence SEQ ID NO: 303).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP164c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 306 (encoded by the miORF164c of sequence SEQ ID NO: 305).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP164c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 306, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172f for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172f regulates the expression of miR172f encoded by a pri-miR172f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172f, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEPI 72f, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEPI 72f, wherein said pri-miR172f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 307, in particular wherein said miR172f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 309 (present on the pre-miR172f of sequence SEQ ID NO: 308).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172f, wherein said miPEP172f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 311 (encoded by the miORF172f of sequence SEQ ID NO: 310).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172f into said parasitic plant, wherein said miPEP172f regulates the expression of miR172f encoded by a pri-miR172f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172f is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172f is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172f.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 307, in particular wherein said miR172f has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 309 (present on the pre-miR172f of sequence SEQ ID NO: 308).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172f has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 311 (encoded by the miORF172f of sequence SEQ ID NO: 310). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172f having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 311 , in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 312, in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 314 or 315 (present on the pre-miR172a of sequence SEQ ID NO: 313).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172a, wherein said miPEP172a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 317 (encoded by the miORF172a of sequence SEQ ID NO: 316). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172a into said parasitic plant, wherein said miPEP172a regulates the expression of miR172a encoded by a pri-miR172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 312, in particular wherein said miR172a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 314 or 315 (present on the pre-miR172a of sequence SEQ ID NO: 313).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 317 (encoded by the miORF172a of sequence SEQ ID NO: 316).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 317, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 318, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 320 (present on the pre-miR172b of sequence SEQ ID NO: 319).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172b, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322 (encoded by the miORF172b of sequence SEQ ID NO: 321).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172b into said parasitic plant, wherein said miPEP172b regulates the expression of miR172b encoded by a pri-miR172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 318, in particular wherein said miR172b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 320 (present on the pre-miR172b of sequence SEQ ID NO: 319).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322 (encoded by the miORF172b of sequence SEQ ID NO: 321).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 322, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172c for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172c, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 323, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 325 or 326 (present on the pre-miR172c of sequence SEQ ID NO: 324).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172c, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 328 (encoded by the miORF172c of sequence SEQ ID NO: 327).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172c into said parasitic plant, wherein said miPEP172c regulates the expression of miR172c encoded by a pri-miR172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172c.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 323, in particular wherein said miR172c has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 325 or 326 (present on the pre-miR172c of sequence SEQ ID NO: 324).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172c has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 328 (encoded by the miORF172c of sequence SEQ ID NO: 327).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172c having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 328, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172d for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172d, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 329, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 331 or 332 (present on the pre-miR172d of sequence SEQ ID NO: 330).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172d, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 334 (encoded by the miORF172d of sequence SEQ ID NO: 333).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172d into said parasitic plant, wherein said miPEP172d regulates the expression of miR172d encoded by a pri-miR172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172d.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 329, in particular wherein said miR172d has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 331 or 332 (present on the pre-miR172d of sequence SEQ ID NO: 330).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172d has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 334 (encoded by the miORF172d of sequence SEQ ID NO: 333). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172d having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 334, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172g for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172g regulates the expression of miR172g encoded by a pri-miR172g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172g, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172g, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172g, wherein said pri-miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 335, in particular wherein said miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 337 or 338 (present on the pre-miR172g of sequence SEQ ID NO: 336).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172g, wherein said miPEP172g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 340 (encoded by the miORF172g of sequence SEQ ID NO: 339). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172g into said parasitic plant, wherein said miPEP172g regulates the expression of miR172g encoded by a pri-miR172g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172g is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172g is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172g.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 335, in particular wherein said miR172g has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 337 or 338 (present on the pre-miR172g of sequence SEQ ID NO: 336).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172g has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 340 (encoded by the miORF172g of sequence SEQ ID NO: 339).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172g having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 340, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP319a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP319a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 343 or 344 (present on the pre-miR319a of sequence SEQ ID NO: 342).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319a, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346 (encoded by the miORF319a of sequence SEQ ID NO: 345).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP319a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 343 or 344 (present on the pre-miR319a of sequence SEQ ID NO: 342).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346 (encoded by the miORF319a of sequence SEQ ID NO: 345).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 346, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP319b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP319b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 347, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 349 or 350 (present on the pre-miR319b of sequence SEQ ID NO: 348).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP319b, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352 (encoded by the miORF319b of sequence SEQ ID NO: 351).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP319b into said parasitic plant, wherein said miPEP319b regulates the expression of miR319b encoded by a pri-miR319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP319b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 347, in particular wherein said miR319b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 349 or 350 (present on the pre-miR319b of sequence SEQ ID NO: 348).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP319b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352 (encoded by the miORF319b of sequence SEQ ID NO: 351).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP319b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 352, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP172e for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP172e, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 353, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 355 or 356 (present on the pre-miR172e of sequence SEQ ID NO: 354).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP172e, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358 (encoded by the miORF172e of sequence SEQ ID NO: 357).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP172e into said parasitic plant, wherein said miPEP172e regulates the expression of miR172e encoded by a pri-miR172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP172e.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 353, in particular wherein said miR172e has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 355 or 356 (present on the pre-miR172e of sequence SEQ ID NO: 354).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP172e has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358 (encoded by the miORF172e of sequence SEQ ID NO: 357). According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP172e having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 358, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP396a for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP396a, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 359, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 361 or 362 (present on the pre-miR396a of sequence SEQ ID NO: 360).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396a, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 364 (encoded by the miORF396a of sequence SEQ ID NO: 363). According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP396a into said parasitic plant, wherein said miPEP396a regulates the expression of miR396a encoded by a pri-miR396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP396a.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 359, in particular wherein said miR396a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 361 or 362 (present on the pre-miR396a of sequence SEQ ID NO: 360).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 364 (encoded by the miORF396a of sequence SEQ ID NO: 363).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP396a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 364, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent. According to another embodiment, the subject matter of the invention concerns the abovedescribed use of a miPEP396b for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant. In particular, the subject matter of the invention concerns the above-described use of the miPEP396b, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 365, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 367 or 368 (present on the pre-miR396b of sequence SEQ ID NO: 366).
According to another embodiment, the subject matter of the invention concerns the abovedescribed use of the miPEP396b, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 370 (encoded by the miORF396b of sequence SEQ ID NO: 369).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method for inhibiting the growth of a parasitic plant belonging to Phelipanche species (in particular P. ramosa) comprising a step of externally introducing a miPEP396b into said parasitic plant, wherein said miPEP396b regulates the expression of miR396b encoded by a pri-miR396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant: by watering; by spraying; or by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant. According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10'1 M of said miPEP396b.
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said pri-miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NO: 365, in particular wherein said miR396b has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 367 or 368 (present on the pre-miR396b of sequence SEQ ID NO: 366).
According to another embodiment, the subject matter of the invention concerns the abovedescribed method, wherein said miPEP396b has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 370 (encoded by the miORF396b of sequence SEQ ID NO: 369).
According to another embodiment, the subject matter of the invention concerns the abovedescribed composition comprising from 10'9 M to 10'1 M of a miPEP396b having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NO: 370, in particular said composition being liquid or solid and further comprising an excipient, a diluent or a solvent.
In addition to these various singular aspects and/or singular embodiments described above, the invention includes their combination. In particular, the following uses are described:
- the use of a miPEP for inhibiting the metabolic recovery of parasitic plant seeds belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a;
- the use of a miPEP for inhibiting the radicle protrusion of parasitic plant seeds belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a; - the use of a miPEP for inhibiting the metabolic recovery and (or) the radicle protrusion of parasitic plant seeds belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a;
- the use of a miPEP for inhibiting the haustoriogenesis of parasitic plant seedlings belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of a miR chosen among from: miR319a, miR319b and 396b;
- the use of a miPEP for inhibiting the metabolic recovery and (or) the haustoriogenesis of parasitic plant seeds and (or) seedlings belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a;
- the use of a miPEP for inhibiting the radicle protrusion and (or) the haustoriogenesis of parasitic plant seeds and (or) seedlings belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a; and
- the use of a miPEP for inhibiting the metabolic recovery, the radicle protrusion and (or) the haustoriogenesis of parasitic plant seeds and (or) seedlings belonging to Phelipanche species (in particular P. ramosa), wherein said miPEP regulates the expression of miR319a, which can be declined as are the different singular aspects and/or singular modes of realisation described above (/.e. use, method, composition).
In any event, it should be noted that the various aspects of the invention, as well as the various embodiments thereof, are interdependent. These can therefore be combined with each other to obtain preferred aspects and/or embodiments of the invention not explicitly described. This is also true for the set of definitions provided in this description, which applies to all aspects of the invention and its embodiments.
Furthermore, the present invention is illustrated, but not limited to, the following Figures and Examples.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1 Stimulant-induced seed germination of O. cumana involves regulation of miRNA target gene expression. Relative expressions were measured by quantitative PCR using RNA isolated from O. cumana seeds treated with mock (control) or 0.1 pM (±)-GR24 during 48 hr. UBI10 was used as the reference gene and expression in GR24-treated seeds was normalized to gene expression in mock-treated seeds (AACt). Data are means ± standard deviation (n = 3). Asterisks indicate statistical differences compared with mock-treated seeds (Tukey honestly significant difference; **P < 0.01 and ***P < 0.001).
Figure 2 Several miPEPs affect the germination of O. cumana seeds, (a) Visualization through a stereo microscope of metabolic activity recovery and radicle protrusion after MTT staining of seeds, (b) Percentage inhibition of metabolic activity recovery (white bars) and radicle protrusion (black bars) of seeds 54h after a co-treatment with 0.1 nM (±)-GR24 and 100 pM miPEPs. Only miPEPs with significant effects according to Tukey-HSD test are shown. Error bars represent SEMs; asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; *P < 0.05, **P < 0.01 , and ***P < 0.001).
Figure 3 Few miPEPs affect the germination of P. ramosa seeds. Percentage inhibition of metabolic activity recovery (white bars) and radicle protrusion (black bars) of seeds 96h after a co-treatment with 0.01 nM (±)-GR24 and 100 pM miPEPs. Asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; ***P < 0.001); Error bars represent SEMs.
Figure 4 miPEP319a affects broomrape seed germination. The percentages of radicle protrusion (a, c, e, and g) and of metabolic activity recovery (b, d, f, and h) of O. cumana (a, b, c, and d) and P. ramosa (e, f, g, and h) seeds were determined after treatment with the relevant miPEP319a (a, b, e, and f) or miPEP319b (c, d, g, and h). Means and standard errors were determined from three biological replicates. Asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; *P < 0.05 and ***P < 0.001); Error bars represent SEMs.
Figure 5 miPEP319a regulates expression of its associated miRNA target genes and pri- miRNA. Relative expressions were measured by quantitative PCR using RNA isolated from O. cumana seeds treated with mock (control) or 0.1 nM (±)-GR24 with or without 100 pM miPEP during 48 hr. UBI10 was used as the reference gene and expression in GR24-treated seeds was normalized to gene expression in mock-treated seeds (AACt). Data are means ± standard deviation (n = 3). Different letters indicate significantly differences with negative control according to ANOVA test (P<0.05).
Figure 6 miPEP319a down-regulates the CYP707A 1 expression induced by a (±)-GR24 treatment. Relative expressions of CYP707A 1 were measured by quantitative PCR using RNA isolated from O. cumana seeds treated (a) with mock (control) or 0.1 pM (±)-GR24 and (b) with mock (control) or 0.1 nM (±)-GR24 with or without a 100 pM miPEP treatment during 12 hr. UBI10 was used as the reference gene and expression in GR24-treated seeds was normalized to gene expression in mock-treated seeds (AACt). Data are means ± standard deviation (n = 3). Different letters indicate significantly differences with negative control according to ANOVA test (P<0.05).
Figure 7 miPEP319 treatments affects the number of O. cumana attachments on sunflower roots. Sunflowers were cultured in a substrate containing O. cumana seeds. The positive control corresponds to sunflowers cultured in the presence of broomrape without application of miPEP. The effect of miPEPs is characterized by counting attachments after 5 weeks of coculture. Data are means ± standard deviation (n = 10). Results with different letters are significantly different from each other (P<0.05) based on ANOVA.
Figure 8 Percentage inhibition of metabolic activity recovery (white bars) and radicle protrusion (black bars) of O. cumana seeds 54h after a co-treatment with 1 nM (a) or 10 nM (b) (±)-GR24 and 100 pM miPEPs. Only miPEPs with significant effects according to Tukey-HSD test are shown. Error bars represent SEMs; asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; **P < 0.01 , and ***P < 0.001).
Figure 9 Percentage inhibition of metabolic activity recovery (white bars) and radicle protrusion (black bars) of P. ramosa seeds 96h after a co-treatment with 0.1 nM (a) or 1 nM (b) (±)-GR24 and 100 pM miPEPs. Asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; ***P < 0.001); Error bars represent SEMs.
Figure 10 miPEP319a affects the germination of O. cumana seeds whatever the population of the parasite or the germination stimulant applied. The percentages of radicle protrusion (a, c, e, g, i, k, m, and o) and of metabolic activity recovery (b, d, f, h, j, I, n and p) of O. cumana seeds from Oc49 (a to h) and OclN23 (i to p) populations were determined 56 h after treatment with miPEP319a (a, b, e, f, i, j, m, and n) or miPEP319b (c, d, g, h, k, I, o, and p) alone or with DCL (a, b, c, d, i, j, k, and I) or with sunflower root exudates (e, f, g, h, m, n, o, and p). Means and standard errors were determined from three biological replicates. Asterisk indicate a significant difference between test conditions and controls according to Tukey-HSD test (n = 3; **p<0.01 and ***P < 0.001). Error bars represent SEMs.
Figure 11 Some miPEPs regulates expression of their associated miRNA target genes.
Relative expressions were measured by quantitative PCR using RNA isolated from O. cumana seeds treated with mock (control) or 0.1 nM (±)-GR24 with or without 100 pM miPEP during 48 hr. UBI10 was used as the reference gene and expression in GR24-treated seeds was normalized to gene expression in mock-treated seeds (AACt). Data are means ± standard deviation (n = 3). Different letters indicate significantly differences with negative control according to ANOVA test (P<0.05).
Figure 12 A miPEP319 treatment does not affect the growth of sunflower. Sunflowers were treated with miPEP319a and miPEP319b in order to verify the absence of negative effects on their growth. Measurements of the size of the sunflowers were carried out 5 weeks after sowing. Data are means ± standard deviation (n = 10). Results with different letters are significantly different from each other (P<0.05) based on ANOVA.
EXAMPLES miPEPs inhibit seed germination of both parasitic plant species, Orobanche cumana and Phelipanche ramosa
Materials and methods
Plant material, seed sterilization, and root exudate production
O cumana seeds (seed lot Oc49) were collected in 2017 in a sunflower field (Helianthus annuus) at Longeville-sur-Mer (France). Seed lot OclN23, collected in Guadalquivir (Spain), were provided by LI PM from Toulouse (France). P. ramosa seeds were collected in Saint Martin de Fraigneau (France) in an oilseed rape field. Sunflower seeds (accession 2603 susceptible to O. cumana) were provided by LI PM.
Broomrape and sunflower seeds were surface-sterilized according to Lechat et al. (2012, Journal of Experimental Botany 63(14): 5311-5322) using 2,4% (v/v) sodium hypochlorite for 7 min for seeds of O. cumana and sunflower, and 5 min for P. ramosa. Seeds were successively rinsed three times for 30 sec and three times for 5 min with sterile distilled water. For the production of root exudates, seedlings of sunflower were disposed into a drained plastic pot [700 ml, 9 x 11 x 12 cm (WxLxH), Nicoplast] nested in an undrained pot and filled with glass beads (03 mm) covered by a 1 cm layer of vermiculite. The pot systems were deposited in a growth chamber at 21°C with a 16h light/8h dark photoperiod. Plantlets were first watered with 200 ml of half-strength TT medium and the volume was renewed every two days. Exudates were collected after 6 weeks, then concentrated on Strata-X 33u Polymeric Reversed Phase (Phenomenex) and eluted with 6 mL of acetonitrile. Eluate was evaporated and the remaining liquid was diluted with 1 volume of 100 % acetonitrile.
Peptide assays Peptides were synthesized by sb-PEPTIDE (sb-peptide.com) and dissolved at 10 mM in water or in 50 % acetonitrile (stock solution), aliquoted and conserved at -80°C. Seeds were treated with a 1 to 100 pM final concentration of miPEP diluted in water before use.
Table 1. Sequence of miPEPs in 0. cumana and P. ramosa.
Seed germination assays and miPEP treatments
Surface-sterilized seeds were conditioned in the incubation solution (1 mM HEPES buffer; pH 7.5 and 0.1 % p/v of Plant Preservative Mixture) in 50 mL tubes during 7 days in the dark at
21 °C and 25 °C for P. ramosa and O. cumana, respectively. Once conditioned, 50 pL of conditioned seed (about 150 seeds) were distributed in 96-well plates. Ten microliters of germination stimulants [(±)-GR24, a synthetic strigolactones, dehydrocostus lactone (DCL), a sequiterpene lactone or exudates] and 10 pl of miPEP solution were added, and the final volume was adjusted to 100 pl with sterile water. The incubation time was 56h and 96h for O. cumana et P. ramosa, respectively. Metabolic resumption and radicles protrusion of seeds were then observed 18h after MTT staining (Pouvreau et al., 2013, Plant Methods 9(1): 32). Germination assays for RNA extraction, germination stimulation and miPEP treatments were performed in 24 well-plates with 7 mg of seeds in a final volume of 500 pL per well. RNA extraction and qRT-PCR
Total RNA was extracted from 14 mg of seeds using the NucleoSpin RNA Plant kit (Macherey- Nagel) with small modifications. The steps corresponding to DNAse treatment and subsequent washing were omitted. RNA was eluted with 50 pL of RNase-free water and a DNase I digestion was then performed (Qiagen). RNA was finally purified using NucleoSpin RNA
Clean-up XS (Macherey-Nagel) and collected in 30 pL of RNase-free water. cDNAs were synthesized from 400 ng of total RNA using qScript cDNA supermix (Quantabio) for miRNA target genes expression analysis and from 1 pg using qScript cDNA Flex synthesis (Quantabio) for precursor miRNA expression analysis. qPCR was performed with the PerfeCTa SYBR Green SuperMix ROX (Quantabio). The polyubiquitin gene UBI10 was used as the reference gene and primer sequences for qPCR are listed in the below data Table 2.
Table 2. List of miRNA target genes in 0. cumana. Pot experiments
Sunflower seeds were placed on a 13 cm circular filter paper (Macherey-Nagel) in a sterile circular Petri dish (150 x 15 mm), and covered with half a filter paper. Thirteen mL of sterile distilled water are added to the Petri dish which were placed vertically at 21 °C with a 16 h photoperiod. Once the sunflower seeds have germinated, one seed was sown per 500 mL pot filled with a sand (5% of free silica)-vermiculite mixture (v/v) and 30 mg of O. cumana seeds. Control experiments were done without O. cumana seeds. Sunflowers were cultivated in a photoperiod of 16 h day / 8 h night and watered 3 times per week with 10 mL of half Long Ashton watering solution containing (control) or not 1 pM of miPEP. A watering complement was done 7 h after miPEP treatments with a half Long Ashton solution. The broomrape attachment count was conducted 5 weeks after sowing.
Statistical analyses
Statistical analyses were done with R Software (rproject.org) and were performed using analysis of variance and multiple confidence interval testing.
Data corresponding to inhibition rate of germination were subjected to pairwise comparison analysis (Tukey-HSD) with confidence intervals of 99.9, 99, and 95 %. The mean values were ± SEM with n = 3.
Target gene expression data from seeds treated or not with (±)-GR24 were subjected to pairwise comparisons (T ukey-HSD) between treatment and negative control (solvent treatment with 0.1 % (v/v) of acetonitrile) and with a confidence interval of 99.9 % and 99 %. Means are values ± SD with n = 3.
Gene expression data from seeds treated with (±)-GR24 and miPEP were subjected to analysis of variance (ANOVA) with a 95 % confidence interval. Means are values ± SD with n = 3.
Results
Identification of miRN A, their targets and miPEPs in Orobanche cumana
Conserved sunflower broomrape miRNAs were identified by searching by blast for sequences highly homologous to Arabidopsis thaliana miRNAs (miRbase) in O. cumana genome (heliagene.org/OclN23-20190821/). Corresponding miR* were further searched and analysis of stem loop structure were performed using RNAfold (rna.tbi. univie. ac.at/cgi- bin/RNAWebSuite/RNAfold.cgi). We focused our analysis on miRNA families conserved in most of green plants, i.e. from miR156 to miR399. We were able to identify 39 miRNAs, corresponding to miR156 to miR396 (Accession numbers OP961563 to OP961601). Interestingly, we were not able to identify miRNAs from the families 157, 158, 161 , 162, 163, 391 , 397, 398, and 399.
Due to the lack of available data concerning the expression of miRNA gene, we identified 5’ genomic sequences close to pre-miRNAs and searched for ORFs corresponding to miPEPs (Lauressergues et al., 2015, Nature 520(7545): 90-93). Previous analyses revealed a 5’ arm median length (363 bases) leader sequence of A. thaliana pri-miR. We searched miPEP ORF located close to the pre-miRNA within this window. Thus, 39 miPEPs were identified and synthetized (Table 1).
The target genes of miRNA conserved in A. thaliana were searched in the genome of O. cumana using blast modules. The miRNA binding sites were searched in the transcripts of the target genes by sequence alignment. Only target genes whose binding site had a maximum of five mismatches and no more than two in a row were retained. Additional verification was performed on O. cumana target gene sequences using psRNATarget software (Dai et al., 2018, Nucleic Acids Research 46(W1): W49-w54) by blasting A. thaliana miRNAs. By applying this strategy, 37 mRNA targets for 13 of the 16 identified miRNAs families were identified (Table 2).
Expression of miRNA target transcripts during strigolactone-induced seed germination in O. cumana
Analysis of the transcriptome evolution during strigolactone-dependent germination of broomrape seeds has been the subject of several studies and has essentially revealed that it is a limited molecular shift (Lechat et al., 2012, Journal of Experimental Botany 63(14): 5311- 5322). Gene expression regulated by miRNAs during plant seed germination has also been widely studied (Tognacca & Botto, 2021 , Plant Communications 2(4): 100169). We therefore investigated this miRNA-associated expression pattern in the particular case of a chemically induced seed germination. For this, the relative expression of the target genes of the 13 miRNA families was determined 48 h after treatment of conditioned O. cumana seeds with 0.1 pM (±)- GR24 (Fig. 1). Among the 37 target genes studied, 23 were significantly up-regulated, 3 were down-regulated, and 11 do not show differential expression. Four genes are overexpressed more than 20 times compared to the control: OcSPLIO target of miR156, OcARFW target of miR160, OcCUC1/2b target of miR164, and OcAP2b target of miR172 exhibiting a 368.7 ± 0.21-fold upregulation. Some miPEPs affect the chemically-induced seed germination of broomrapes
Thus, most of the miRNA target genes are up-regulated during induced germination of O. cumana seeds. Therefore, it can be expected that the corresponding miRNAs are down- regulated during this biological process. If miPEPs stimulate the expression of their respective miRNAs and thus inhibit those of their target genes, an exogenous addition may then impact germination and probably inhibit it. To test this hypothesis, the 39 miPEPs generated from the O. cumana genome analysis were assayed on germination. As a preamble, a first screening consisted of testing the 39 miPEPs on conditioned but unstimulated (±)-GR24 seeds to assess their ability to mimic the effect of germination stimulants on seeds. None of them induced seed germination of this root parasitic plant. Then, seeds were treated concomitantly with (±)-GR24 and each miPEP at 100 pM. The concentration of 1 pM in (±)-GR24 usually used for germination assays leads to a saturation of the germination process and does not allow to visualize the potential inhibitory effect of a miPEP. In order to overcome this saturation effect, the germination assay was performed at lower concentrations of germination stimulant. Thus, three concentrations of (±)-GR24 (0.1 , 1 and 10 nM), equivalent of the EC50 as previously described (Pouvreau et al., 2021 , Methods in Molecular Biology 2309: 59-73), were used to screen the effects of miPEPs during germination. Two parameters of seed germination were evaluated 56h after co-treatment, the inhibition rate of metabolic activity recovery and of radicle protrusion (Fig. 2, Fig. 8). The 0.1 nM (±)-GR24 concentration is the one allowing the highest inhibitory activity of the tested miPEPs. However, a compilation of the data obtained for the three concentrations reveals that a core set of 11 miPEPs (miPEP159, miPEP160a,b,c,d, miPEP164, miPEP169b, miPEP172a,b, miPEP319a, and miPEP393b) related to 7 miRNA family (mir159, mir160, mir164, mir169, mir172, mir319, and mir393) exhibits inhibitory activity on radicle protrusion while only miPEP319a and miPEP393b inhibit both metabolic activity recovery and radicle protrusion, whatever the (±)-GR24 concentration. Overall, these results indicate that miPEP319a has the strongest inhibitory activity on the germination of O. cumana seeds. miR319a and miR319b are two different pri-miRNAs producing the same mature miRNA. It is interesting to note that miP319b has no effect on this process demonstrating the miRNA-specific nature of the mode of action of miPEPs. The impact of a miPEP on the expression of its miR may result in the inhibition of the genes targeted by said miR, however, does not predict its inhibitory effect on the phenotype.
To investigate if the observed effects of miPEPs on O. cumana seed germination were species specific, we also tested some miPEPs on the seed germination of another broomrape species, Phelipanche ramosa. Despite the absence of a genome available for this parasitic species, a non-exhaustive study could be carried out using some genomic data produced by S. Wicke (Humbold University of Berlin) and used to study another key process of broomrape development, the haustorium formation (unpublished results). We searched for miRNAs corresponding to miR156, 172 and 319 families in these data, and we were able to identify 5 miRNAs related to miR172 genes, 2 to miR319 and 7 to miR156 (Accession numbers OP965504 to OP965517). Indeed, these three miRNA families were selected because they correspond to miPEPs with an inhibitory effect on O. cumana seed germination (Fig. 1) and to miRNAs related to the cytokinin response pathway, hormones known to be involved in haustorium formation in P. ramosa (Curaba etal., 2014, Journal of Experimental BotanyQ5(Q . 1425-1438; Goyet et al., 2017, Journal of Experimental Botany 68(20): 5539-5552). We next identified and synthetized putative corresponding miPEPs (Table 1), and, as in the case of O. cumana, the 14 miPEPs applied alone without germination stimulant did not stimulate germination of P. ramosa seeds. Seeds, previously disinfected and conditioned for 7 days at 21°C in the dark, were then treated concomitantly with 0.01 , 0.1 or 1 nM (±)-GR24 and each miPEP at 100 pM. Inhibition rates of metabolic activity recovery and of radicle protrusion were evaluated 96h after co-treatment (Fig. 3, Fig. 9). Among the 14 miPEPs, only miPEP319a exhibits a strong inhibitory effect on P. ramosa seed germination whatever the (±)-GR24 concentration, whereas miPEP319b had no effect. This result clearly demonstrates the versatile nature of one of the miR319-related miPEPs in its ability to inhibit seed germination in broomrapes. It is interesting to note the miRNA-specific nature of the action of miPEPs since of the two members of the miPEP319 family, one is strongly inhibitory while the second is inactive in both species. This was confirmed by performing germination assays with varying concentrations of (±)-GR24 and miPEP (Fig. 4). At a concentration of 100 pM, miPEP319a induced a total inhibition of radicle protrusion in both broomrape species regardless of (±)- GR24 concentration, accompanied by a partial or total inhibition of metabolic recovery in O. cumana and P. ramosa, respectively. In contrast, miPEP319b had only a slight effect only at low concentration of (±)-GR24 and high concentration of peptide. In order to verify that the effects induced by these miPEPs during the germination of O. cumana seeds are not specific to the seed stimulation by a strigolactone, the same experiment was performed with a natural germination stimulant present in sunflower exudates, dehydrocostus lactone (DCL), a guaianolide sesquiterpene lactone (Joel et al., 2011 , Phytochemistry 72(7): 624-634) (Fig. 10). The range of DCL concentrations is the same as the one used in (±)-GR24 for the previous tests. The application of DCL results in the same inhibition profiles by miPEP319a as those obtained with strigolactones, indicating that probably the same metabolic pathway was targeted by the peptide. Root exudates from the sunflower accession 2603, known to be susceptible to broomrape, were also used in order to simulate as much as possible the natural conditions of germination induction of O. cumana seeds by the host plant. Indeed, these root exudates are likely to contain the two types of germination stimulants, strigolatones and sesquiterpene lactones (Ueno et al., 2014, Phytochemistry 108: 122-128). Three successive dilutions to the tenth in root exudate were used. Exudates induce a response similar to that of (±)-GR24 or DCL following treatment with miPEP319a (Fig. 10). The inhibitory effect on radicle protrusion and metabolic recovery is strong at the highest miPEP concentration. In contrast, miPEP319b does not induce significant changes.
The effect of miPEP319 was also characterized on another race of O. cumana to confirm that the germination inhibitory effects observed for miPEP319a are not race-specific. The OclN23 population was collected in Spain in the Guadalquivir Valley and corresponds to the race F, while the French Oc49 population is a race E. (±)-GR24 was not used on this seed lot because they germinate poorly with this synthetic stimulant, making visualization of a potential inhibitory effect of miPEP very difficult. Inhibition of radicle protrusion is observed for all concentrations of miPEP319a and DCL used (Fig. 10). MiPEP319a at the highest concentration also induces inhibition of radicle protrusion when root exudates from susceptible sunflowers are used instead of DCL. Inhibition of metabolic recovery is observed for all concentrations of DCL, or root exudates used with the highest peptide concentration. miPEP319b has no effect on germination of the seeds of this lot, used in co-treatment whether with DCL or root exudates.
Impact of miPEP on gene expression
To know whether the inhibition of O. cumana seed germination triggered by some miPEPs was mediated through a miRNA process, we first analyzed the expression of their corresponding target genes following a peptide treatment. Thus, a treatment of the miPEP159, miPEP164, miPEP172a,b, and miPEP319a, that strongly inhibited seed germination of O. cumana, induced concomitantly a strong down regulation of at least one their associated target genes (Fig. 11), suggesting a post-transcriptional regulation by miRNAs. However, in the absence of data on the expression of mature miRNAs and/or pri-miRNAs, transcriptional regulation of these targets without the intervention of miRNA cannot be excluded as has been demonstrated in A. thaliana (Das et al., 2015; Sarkar Das etal., 2018). To address this, we focused our study on miPEP319a, the most effective peptide, by analyzing the expression of the pri-miR319a and its corresponding target genes OcTCP2, OcTCP4, OcMYB33a, and OcMYB33b (Fig. 5). Quantification of expression was done for up to 48 hours after treatment with 0.1 nM (±)-GR24 concomitantly or not with 100 pM miPEP319a or miPEP166c as negative control. First of all, it should be noted that (±)-GR24 leads to an accumulation of the target gene mRNAs as early as 6 h after treatment and which is maintained or increases up to 48 h with the exception of OcTCP2 which shows a transient upregulation. In each case, application of the active synthetic miPEP319a induces a significative decrease of the corresponding mRNA, which is not the case with the inactive miPEP166c. Regarding the pri-miR319a, a decrease in accumulation is observed 6 h after (±)-GR24 addition, which coincides interestingly with the upregulation of all four target genes. Again, the addition of miPEP319a (but not of miPEP166c) impacts the expression profile since it clearly limits the downregulation of its pri-miRNA. Altogether, these results suggest that the chemically induced seed germination of O. cumana involved a regulation of the expression of some transcription factors mediated by their miRNA gene. Indeed, throughout the first hours of the chemically induced seed germination of O. cumana, the accumulation of pri-miR319a is synchronized and inversely proportional to that of its target genes. Moreover, the effects of a miPEP319a treatment on accumulation of pri-miR319a, downregulation of its target genes, and seed germination rate further support this hypothesis.
It is known that seed dormancy release and thus germination in plant rely mainly on ABA catabolism by specific ABA 8'-hydroxylases encoded by the cytochrome CYP707A gene family. In root parasitic plants, it has been shown that the activation of the expression of these genes is dependent on germination stimulants (Brun etal., 2019, Plant Cell Environment 42(9): 2612-2626). To investigate whether this molecular process leading to seed germination is under the control of miR319 and its associated target genes in O. cumana, the expression of CYP707A 1 was studied after treatment with miPEP319a (Fig. 6). First, as expected, 0.1 pM (±)-GR24 induces a strong and rapid accumulation of CYP707A 1 mRNA (Fig. 6a). To observed a potential effect of miPEP319a, seed germination was induced with a lower (±)- GR24 concentration of 0.1 nM explaining a global lower CYP707A 1 expression level (Fig. 6b). Treatment of O. cumana seeds with synthetic miPEP319a decreases the accumulation of CYP707A 1 3h after application suggesting that expression of this gene is directly or indirectly under the control of transcription factors, themselves under the control of miR319.
Taken together, these results show that, among all the identified and tested miPEPs, miPEP319a is a good candidate to develop a new biocontrol agent against O. cumana. In order to test this hypothesis, the innocuity of miPEP319a and b on the development of sunflowers was first verified by watering them three times a week with a solution containing 1 pM of peptide. Size of the sunflowers were not impacted after 5 weeks of cultivation in pots demonstrating the absence of deleterious effect of both miPEP319 on sunflower development (Fig. 12). Then, co-culture experiments in pots were performed in the same conditions and number of parasite attachments on sunflower roots was measured 5 weeks after sowing. The addition of miPEP319a or miPEP319b to the substrate watering solution induces a significant decrease in the number of broomrape attachments on sunflowers, compared to sunflowers co- cultured with O. cumana seeds watered with the nutrient solution alone (Fig. 7). Both miPEPs thus reduce sunflower infestation by O. cumana under these pot culture conditions. In the case of miPEP319a, this reduction can be attributed to the inhibitory effect of the synthetic peptide on the seed germination rate then significantly reducing the number of parasites in the rhizospere. Concerning miPEP319b, the observed reduction was less expected since this peptide has never shown any inhibitory effect on O. cumana germination. However, it cannot be excluded that this peptide induced other mechanisms leading to a lower number of tubercles attached to the roots of the host plant. We can thus suggest an inhibitory effect on the formation of the haustorium, the key organ in the invasive process of the parasite, or an indirect activation of defense mechanisms in the host.
Conclusion
Here, 37 mRNA targets for 13 of 16 identified miRNAs families were identified. Indeed, it was not possible to find potential target sequences for miR168, miR390 and miR395. At first, the relative expression of the 37 target genes was determined 48 h after treatment of conditioned O. cumana seeds with 0.1 pM (±)-GR24 (Fig. 1). Among the target genes studied, 23 were significantly up-regulated, 3 were down-regulated, and 11 do not show differential expression. The fact that the expression of the three genes 0cGRF3, 0cGRF5 and OcPHV/PHBa is down- regulated may suggest that the corresponding miRNAs, miR396 and miR165/166, may naturally be involved in the chemically-dependent process of seed germination in O. cumana. Because these miRNAs are not known to be involved in seed germination in autotrophic plants such as A. thaliana (Das et al., 2015), these results require further study.
Thirty-nine miPEPs were generated from the O. cumana genome analysis and assayed on germination. None of them were able to induced alone the seed germination while a core set of 11 miPEPs belonging to 7 miRNA family (mir159, mir160, mir164, mir169, mir172, mir319, and mir393) exhibits a strong inhibitory activity on the seed germination induced by (±)-GR24. This exhibits that these miRNAs may be involved in the seed germination process of O. cumana, as previously demonstrated in non-parasitic plants (Curaba et al., 2014, Journal of Experimental Botany 65(6) : 1425-1438) .
An ABA treatment of Arabidopsis or broomrape seeds inhibits their germination (Pouvreau et al., 2013, Plant Methods 9(1): 32). In Arabidopsis, it has been shown that this treatment induced a miR159 up-regulation associated with MYB33 ar\d MYB101 mRNA cleavage (Reyes & Chua, 2007, The Plant Journal 49(4): 592-606). In O. cumana, miPEP159 induced an inhibition of seed germination through a total inhibition of radicle protrusion (Fig. 2), concomitantly to a down-regulation of the expression of the three miR159 target genes, OcMYB101, OcMYB33a and OcMYB33b (Fig. 11). Similarly, miPEP319a induces inhibition of seed germination, marked by activation of pri-miR319a expression and repression of expression of both OcMYB33a and OcMYB33b genes (Fig. 5). Both miPEP treatments of O. cumana seeds would therefore act by mimicking the effects of an ABA treatment. Germination assays with a range of O. cumana miPEP319 concentrations, coupled with a range of concentrations of (±)-GR24, DCL or root exudates, confirmed the inhibitory effect of miPEP319a on radicle protrusion regardless of the germination stimulant and the lot of O. cumana seeds used (Fig. 10). Thus, there would be a common response pathway to the different germination stimulants, involving miR319. The miPEP319b of O. cumana shows only a very weak inhibitory effect on radicle protrusion at the lowest concentration of (±)-GR24 or DCL coupled with the highest concentration of peptide. In addition, this miPEP has no effect on metabolic recovery of seeds reflecting a lesser effect compared to miPEP319a. P. ramosa miPEP319a induced similar effects to O. cumana with strong inhibition of germination following treatment with miPEP319a versus a weak effect for miPEP319b. The involvement of miR319 in germination would thus be conserved between these two holoparasitic plants with different effects between members of the same family. miPEP319a can then disrupt the germination process of broomrapes by specifically reducing the expression of its associated miRNA target genes. Interestingly, in this study, it has been demonstrated that the GR24-dependent CYP707A1 expression can be down regulated by a treatment with miPEP319a, suggesting that the corresponding miR319 may interact with this signaling pathway in O. cumana. Analysis with the psRNAtarget server (Dai et al., 2018, Nucleic Acids Research 46(W1): W49-w54) revealed that CYP707A 1 is not a primary target of miR319a. This implies that miR319a could induce the mRNA degradation of at least one of the transcription factors TCP or MYB, leading to a modulation of the expression level of its target genes, and then finally to the down regulation of CYP707A 1. In the absence of effective functional genomics tools for root parasitic plants, the demonstration of this hypothesis remains unlikely. However, it has been shown that transcription factors of the TCP family are component of strigolactones signaling pathway in pea (PsBRCI), bread wheat (TaTB1), and Arabidopsis (AtBRCI and AtTCPI) for other physiological processes than germination (Braun et al., 2012, Plant Physiology 158(1): 225-238; Liu et al., 2017, Plant Physiology 174(3): 1931- 1948; Wang et al., 2020, Nature 583(7815): 277-281). It is therefore tempting to propose that a TCP transcription factor targeted by miR319a is a positive actor in the germination signaling pathway of O. cumana, which could explain the inhibitory effect of miPEP319a on this process. The control of plant pathogens is an ongoing effort that requires constant investment by scientists, especially as the effective tools available are increasingly limited due to growing governmental and societal expectations to reduce the use of chemical pesticides and fertilizers. This is especially true for parasitic weeds, against which chemical treatments are in addition mostly ineffective due to the lack of selectivity and the difficulty to employ a strict protocol of application that needs to consider many kinetic factors, such as seed germination, root infection and post-attachment development. Despite intense efforts to develop effective agricultural practices, selective control of various parasitic plants is still scarce and ineffective in terms of sustainability. The deciphering of the action of miPEPs and the decrypting of the genome of the root parasitic plant O. cumana have been two major and concomitant events that have contributed to initiate the development of a completely new and cutting-edge technology: the use of peptides (miPEPs) as natural molecules to modulate specific gene expression (without genetic transformation or mutation) in the parasite for its biocontrol. Regarding miPEP formulation study, product development strategies could include sunflower coated seeds with miPEPs increasing sunflower’s resistance against broomrape or freeze- dried miPEPs inhibiting broomrape growth that could be diluted and directly sprayed in fields.
Thus, this study, lays the first foundations of a new and effective phytosanitary method to control broomrape parasitism with highly specific and biodegradable natural substances, the miPEPs.

Claims

1. Use of a miPEP319a for inhibiting the growth of a parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
2. Use of the miPEP319a according to claim 1 , wherein said inhibition of the growth of said parasitic plant is obtained by the inhibition of the germination of said parasitic plant and/or the inhibition of the haustoriogenesis of said parasitic plant.
3. Use of the miPEP319a according to claim 2, wherein said inhibition of the germination is obtained by the inhibition of the radicle protrusion of said parasitic plant and optionally by the inhibition of the metabolic recovery of said parasitic plant.
4. Use of the miPEP319a according to any of claims 1 to 3, wherein said parasitic plant belongs to Orobanchaceae family.
5. Use of the miPEP319a according to claim 4, wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
6. Use of the miPEP319a according to any of claims 1 to 5, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
7. Use of the miPEP319a according to any of claims 1 to 6, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346.
8. Method for inhibiting the growth of a parasitic plant comprising a step of externally introducing a miPEP319a into said parasitic plant, wherein said miPEP319a regulates the expression of miR319a encoded by a pri-miR319a.
9. Method according to claim 8, wherein said miPEP319a is externally introduced into said parasitic plant:
- by watering;
- by spraying; or
- by adding a fertilizer, a compost, a culture substrate or a support in contact with the plant.
10. Method according to claim 8 or 9, wherein said miPEP319a is externally introduced into said parasitic plant in the form of a liquid or solid composition comprising from 10'9 M to 10’1 M of said miPEP319a.
11. Method according to any of claims 8 to 10, wherein said parasitic plant belongs to Orobanchaceae family.
12. Method according to claim 11 , wherein said parasitic plant belongs to Orobanche and Phelipanche species, in particular wherein said parasitic plant is Orobanche cumana or Phelipanche ramosa.
13. Method according to any of claims 8 to 12, wherein said pri-miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 191 or 341 , in particular wherein said miR319a has a nucleic acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the nucleic acid sequence SEQ ID NOs: 193, 194, 343 or 344.
14. Method according to any of claims 8 to 13, wherein said miPEP319a has an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346.
15. Composition comprising from 10'9 M to 10'1 M of a miPEP319a having an amino acid sequence presenting a percentage of identity of at least 80%, in particular at least 90%, with the amino acid sequence SEQ ID NOs: 196 or 346, in particular said composition being liquid or solid and further comprising at least an excipient, a diluent, a solvent or a fixing agent.
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