Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L9/00—Supporting devices; Holding devices
  • B01L9/06—Test-tube stands; Test-tube holders
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/34—Purifying; Cleaning
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2400/00—Moving or stopping fluids
  • B01L2400/04—Moving fluids with specific forces or mechanical means
  • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
  • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2400/00—Moving or stopping fluids
  • B01L2400/04—Moving fluids with specific forces or mechanical means
  • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
  • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N2035/00465—Separating and mixing arrangements
  • G01N2035/00524—Mixing by agitating sample carrier
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N2035/00465—Separating and mixing arrangements
  • G01N2035/00564—Handling or washing solid phase elements, e.g. beads

Definitions

• magnetic particles and in particular of magnetic beads, in the context of purification processes is widespread. This is often, without the following list being exhaustive, the purification of cells or biological macromolecules such as isolated proteins, antibodies and nucleic acids.
• Magnetic particles in particular magnetic beads, are used to purify these cells and/or these biological macromolecules.
• the element to be purified attaches to the magnetic particles, which allows it to be separated from the surrounding environment.
• the protocols allowing these purifications involve, among other things, several stages of successive washing of these magnetic particles.
• the magnetic particles are placed in test tubes with the presence of a washing solution, these tubes being on a rack;
• test tubes are moved one by one from the rack to a shaker
• test tubes are stirred in order to resuspend the magnetic beads in the washing solution and thus wash them;
• test tubes are removed one by one from the stirrer and placed on a magnetic rack. Thanks to the action of the near magnetic field, the magnetic particles are pressed against the walls of the test tubes;
• the soiled washing solution is removed, for example by means of the use of a pipette, and changed by a clean washing solution.
• test tubes are to be treated simultaneously and 5 successive washes are recommended, this is very daunting and the test tubes are handled hundreds of times (24 multiplied by 5 multiplied by 2, therefore 240 manipulations), which which is time consuming and can lead to sample reversals, lab staff fatigue, etc.
• a handling error for example an inversion of samples, will be very difficult, if not impossible, to detect and may distort the entire handling.
• test tubes are intended to be reused.
• this application does not disclose a magnetic laboratory container holder base capable of being linked to said laboratory container holder, nor rotation speeds compatible with washing magnetic particles.
• test tube holder permanently fixed to an axis of rotation.
• laboratory container refers to any laboratory supply likely to contain solutions or suspensions in particular. These may, for example, be test tubes which can be closed by means of a suitable stopper or any other means of closure.
• the interval described as being “between A and B” includes the “A" and "B” terminals.
• the invention therefore relates first of all to a washing device 1, comprising:
• a laboratory container holder 2 comprising M fastening means 3 for laboratory containers, with M>1, said fastening means 3 being arranged along at least one axis (x);
• a stirring means 5 capable of being reversibly linked to said laboratory container holder 2 and capable of inducing rotations along an axis of rotation (y) parallel to said at least one axis (x) of said laboratory container holder 2 when the latter is linked to said stirring means 5;
• a base 6 of laboratory container holder 2 comprising at least one magnet, said base 6 being capable of being reversibly linked to said laboratory container holder 2.
• said laboratory container holder 2 comprises as many fixing means as capacity in terms of laboratory containers.
• a fixing means therefore makes it possible to fix a laboratory container and L fixing means make it possible to fix L laboratory containers.
• said laboratory container holder base 6 2 comprising at least one magnet and said stirring means 5 cannot be assembled.
• the magnetic base 6 will not be able, in any case, to disturb the washing of the magnetic particles, which must be out of any magnetic field during washing. The risk of error at this level is completely eliminated.
• a manipulator will not be able, mechanically, to mount the base 6 on the stirring means 5.
• said washing device 1 is a device for washing magnetic laboratory particles, for example magnetic beads.
• said washing device 1 is a device for washing magnetic laboratory particles, for example magnetic beads, in which said magnetic laboratory particles are not grouped together when the stirring means 5 is linked to said door laboratory containers 2.
• the laboratory magnetic particles have a largest dimension between 10 nanometers and 1 millimeter.
• the laboratory magnetic beads have a diameter between 20 and 100 nanometers.
• the laboratory magnetic particles have a largest dimension between 100 nanometers and 1 millimeter.
• the laboratory magnetic particles have a largest dimension between 200 nanometers and 1 millimeter.
• the laboratory magnetic particles have a largest dimension between 10 nanometers and 100 micrometers.
• the laboratory magnetic particles have a largest dimension between 1 and 5 micrometers.
• the laboratory magnetic particles have a larger dimension of about 2.8 micrometers.
• the laboratory magnetic beads have a diameter between 10 nanometers and 100 micrometers.
• the laboratory magnetic beads have a diameter between 1 and 5 micrometers.
• the magnetic beads have a diameter between 10 nanometers and 1 millimeter.
• the magnetic beads have a diameter between 100 nanometers and 1 millimeter.
• the magnetic beads have a diameter between 200 nanometers and 1 millimeter.
• all the non-electrical parts of said washing device 1 are made of a material which is not susceptible to corrosion.
• all the non-electrical parts of said washing device 1 are made of a plastic material and/or of resin.
• all the non-electrical parts of said washing device 1 are made of a plastic material.
• all the non-electrical parts of said washing device 1 are made of resin.
• all the non-electrical parts of said device 1 are made of a material chosen from the group consisting of polylactic acid (PLA) and glycolized polyester (PETG).
• PLA polylactic acid
• PETG glycolized polyester
• all the non-electrical and non-magnetic parts of said washing device 1 are printed in three dimensions, and are made of a material chosen from the group consisting of polylactic acid (PLA) and glycolized polyester ( PETG).
• PLA polylactic acid
• PETG glycolized polyester
• Three-dimensional printing has the advantage of being very simple to implement. In addition, it also allows the insertion of magnets in the base 6 of the laboratory container holder 2, which makes it possible to produce a unique piece. In addition, certain parts of the stirring means 5 are hollow with cavities allowing the passage of the electric cables and the arrangement of the electric components (motor, etc.), which 3D printing makes it possible to produce. Finally, this limits the non-sealed junctions, and therefore contributes to the sealing/tropicalization of the agitation means 5.
• said base 6 of laboratory container holder 2 comprising at least one magnet is able to be reversibly linked to said laboratory container holder 2 and to collect magnetic laboratory particles within said laboratory containers.
• said laboratory container holder 2 comprises a carrier plate 7 as well as a detachable stabilizing base 8 of said carrier plate.
• the magnetic base 6 can for example be inserted between the carrier plate 7 and the detachable stabilizer base 8.
• the carrier plate 7 can be changed while retaining the same detachable stabilizer base.
• said laboratory container holder 2 comprises a plurality of carrier plates 7 as well as a detachable stabilizer base 8 of said carrier plates 7.
• each carrier plate 7 can be used separately with the stabilizer base 8, in particular to carry out agitation by means of the agitator 5.
• said laboratory container holder 2 comprises a plurality of carrier plates 7 as well as a detachable stabilizing base 8 of said carrier plates 7, said carrier plates being adapted to different laboratory containers.
• said laboratory container holder 2 comprises a plurality of carrier plates 7 as well as a detachable stabilizing base 8 of said carrier plates 7, said carrier plates comprising fixing means 3 different in number of fixing means and/or or in dimensions of fastening means.
• said laboratory container holder 2 comprises a plurality of carrier plates 7 as well as a detachable stabilizer base 8 of said carrier plates 7, and said carrier plates 7 are each of a different color. Thus, they will be very easily differentiable and will save time for the user.
• said device 1 comprises between 2 and 10 different carrier plates 7.
• said device 1 comprises between 2 and 10 carrier plates 7 different and having different colors.
• said carrier plate comprises several legs 4, said fixing means 3 are holes with flats, and said detachable stabilizing base 8 comprises means for fixing to said stirring means 5.
• said laboratory container holder 2 comprises stabilizing means, preferably legs 4.
• the carrier plate 7 can serve as a rack even in the absence of a stabilizing base. 8, as it is stabilized by 4 feet.
• the laboratory container holder 2 consists of said carrier plate 7.
• said laboratory container holder 2 comprises stabilizing means allowing it to hold in a position allowing it to accommodate the laboratory containers vertically to prevent their contents from overturning.
• these stabilizing means are legs 4. This is particularly advantageous, as it allows complete stabilization.
• the laboratory container holder 2 is provided with laboratory containers, it will remain stable, and all the more stable as the number of feet 4 will be high.
• said laboratory container holder 2 comprises a number of feet 4 comprised between 2 and 10, preferably comprised between 3 and 7, and preferably 5.
• said laboratory container holder 2 comprises 3 feet 4.
• said laboratory container holder 2 comprises 5 feet 4.
• M is between 2 and 48, preferably between 6 and 36, preferably between 16 and 24.
• said laboratory container holder 2 comprises fixing means 3 of different kinds, and more precisely which make it possible to fix different laboratory containers.
• the various laboratory containers may in particular have different volume capacities.
• the washing device 1 it will be possible to wash magnetic particles, including within tubes of different capacities within the same laboratory manipulation.
• At least one fixing means 3 is suitable for fixing a laboratory container chosen from the group of laboratory containers with a capacity of 0.5 milliliters, 1.5 milliliters, 2 milliliters, 5 milliliters, 15 milliliters, or 50 milliliters.
• At least two fixing means 3 are suitable for fixing a laboratory container chosen from the group of laboratory containers with a capacity of 0.5 milliliters, 1.5 milliliters, 2 milliliters, 5 milliliters, 15 milliliters, or 50 milliliters.
• At least five fixing means 3 are suitable for fixing a laboratory container chosen from the group of laboratory containers with a capacity of 0.5 milliliters, 1.5 milliliters, 2 milliliters, 5 milliliters, 15 milliliters, or 50 milliliters.
• said magnet is capable of collecting said magnetic laboratory particles within said laboratory containers.
• said magnet is capable of collecting said magnetic laboratory particles within said laboratory containers, said collecting taking place at least 1 millimeter above the bottom of said laboratory container. This embodiment is preferred because if a pipette is used to remove the soiled medium, then removal of the soiled medium without magnetic particle removal will be facilitated.
• At least one fixing means 3, and preferably all of the fixing means 3, are holes capable of mechanically retaining the laboratory containers.
• 3D printing it is possible to support any container by making a corresponding custom-made hole.
• the laboratory container holder 2 comprises the carrier plate 7 as well as the stabilizing base 8. Indeed, in the latter case, it will suffice to print in 3D a new carrier plate 7 to have an entire device adapted to said new very particular laboratory containers.
• these holes have a flat part allowing better mechanical action of said hole on said laboratory containers, they are called in the context of the present application “holes with flats”.
• At least one fixing means 3, and preferably all of the fixing means 3, are holes with flats capable of mechanically retaining the laboratory containers.
• said fixing means 3 are arranged on said x axis. This embodiment is particularly suitable for obtaining strictly identical agitation between the different laboratory containers. Indeed, all laboratory containers undergo exactly the same mechanical stresses during shaking.
• said fixing means 3 are arranged on either side of said at least one axis x. This embodiment is particularly suitable for maximizing the number of fastening means 3 while maintaining an overall size of the laboratory container holder 2 that is reduced.
• said fixing means 3 are arranged along two axes x1 and x2. This embodiment is particularly preferred, insofar as it allows simultaneous agitation of a large number of laboratory containers.
• said fixing means 3 are arranged along two axes x1 and x2, both parallel to the axis of rotation y.
• said fixing means 3 are arranged on the axes x1 and x2, both parallel to the axis of rotation y.
• said fixing means 3 are arranged along two axes x1 and x2, both parallel to the axis of rotation y, and x1 and x2 are equidistant from said axis of rotation y.
• said fixing means 3 are arranged on the axes x1 and x2, both parallel to the axis of rotation y, and x1 and x2 are equidistant from said axis of rotation y.
• said fixing means 3 are numbered N.
• This embodiment is very advantageous, insofar as it allows possible note taking relating to each laboratory container during handling.
• the numbers are arranged in such a way that all the fixing means 3 can be aligned with a single 180° rotation of said laboratory container holder 2. For example, such an embodiment is presented in .
• said stirring means 5 is a rotary stirrer.
• said stirring means 5 is a rotary drum stirrer.
• said stirring means 5 is powered by electric current.
• said stirring means 5 comprises an electric power supply and an electric motor.
• said stirring means 5 comprises an electric power supply, an electric motor, as well as a controller 9.
• said stirring means 5 comprises a display screen 10. This screen can for example display the number of revolutions per minute as well as a countdown.
• the controller 9 makes it possible to predetermine the following parameters: the speed of rotation, determined by the number of rotations per minute when the rotations are complete or the number of "to and fro" per minute when the translations are "back and forth”, as well as the duration of the agitation (in minutes).
• the controller 9 makes it possible to determine the following parameters: the speed of rotation, determined by the number of rotations per minute when the rotations are complete or the number of "to and fro" per minute when the translations are "back and forth", as well as the duration of the agitation (in minutes).
• the controller 9 makes it possible to predetermine the following parameters: the speed of rotation, as well as the duration of the agitation.
• the controller 9 makes it possible to determine the following parameters: the speed of rotation, as well as the duration of the agitation.
• controlling the duration of the agitation is also very important. In fact, too short an agitation can lead to a poor quality wash. Conversely, stirring for too long can lead to degradation of the magnetic particles.
• said stirring means 5 is powered by electric current from a rechargeable battery.
• a rechargeable battery This is particularly advantageous, as the mobility of the agitator is greatly improved.
• the stirrer is plugged into the mains, the stirrer is within a refrigerator, and the power cable passes through the joint of the refrigerator; this is imperfect from the point of view of size, and dangerous (risk of electrocution and short-circuiting, in particular due to humidity).
• said stirring means 5 is able to induce complete rotations. In one embodiment, said stirring means 5 is able to induce complete rotations because there is no mechanical obstacle to said rotations. In one embodiment, said stirring means 5 is able to induce successive complete rotations. This type of rotation will be particularly suitable in general, because it induces very strong turbulence inside laboratory containers, which allows the realization of a quality wash. For example, in the aforementioned application CN207976333, no complete rotation can take place because there are mechanical obstacles limiting the amplitude of the rotation, which is very disadvantageous in the completely different context of the invention.
• said stirring means 5 is able to induce successive complete rotations, and these rotations are carried out at a rate of between 10 and 100 rotations per minute, preferably between 15 and 80 rotations per minute, of preferably between 30 and 70 rotations per minute, preferably between 40 and 60 rotations per minute, preferably 50 rotations per minute.
• said agitation means 5 is able to induce successive complete rotations for a time comprised between 1 minute and 24 hours, preferably comprised between 2 minutes and 10 hours, preferably comprised between 30 minutes and 5 hours. . It is important that the duration is sufficient to carry out a good wash, without being too long so as not to damage the magnetic particles.
• said stirring means 5 is able to induce successive complete rotations, and these rotations are carried out at a rate of between 10 and 100 rotations per minute and for a time of between 1 minute and 24 hours.
• said stirring means 5 is able to induce rotations of the "back and forth" type according to an amplitude of between 10 and 180 degrees. This type of rotation will be particularly suitable in case of fear concerning the tightness of said laboratory containers.
• said base 6 comprises a plurality of magnets.
• said magnets are of grade N52.
• the attraction force of said magnets is between 1kg and 100kg, preferably between 10kg and 40kg, preferably around 25kg.
• said magnets are permanent magnets. This is very advantageous: the magnetic field is continuous, it does not cause electricity consumption or variations over time.
• the three dimensions of at least one magnet correspond to the following intervals: 5-15 millimeters, 15-25 millimeters and 30-50 millimeters.
• the three dimensions of the magnets correspond to the following ranges: 8-12 millimeters, 18-22 millimeters and 35-45 millimeters.
• At least one magnet has a smallest dimension greater than 3 millimeters, preferably greater than 5 millimeters, preferably greater than 7 millimeters.
• all of the magnets have a smallest dimension greater than 3 millimeters, preferably greater than 5 millimeters, preferably greater than 7 millimeters.
• said base 6 comprises an alternation of magnets and recessed parts compatible with the feet 4 of the laboratory container holder 2. This embodiment is particularly preferred, because it allows total stabilization of the laboratory container holder 2 within said base 6.
• the magnets are arranged in such a way that the magnetic environment of the interior of the laboratory containers is identical from one laboratory container to another. This embodiment is particularly preferred, because it will allow in particular perfect repeatability and identity between the different laboratory containers with regard to the collection of magnetic particles.
• said stirring means 5 is physically separate. In particular, it is not attached to a work surface, which allows it to be moved easily.
• the distance between each fixing means and the axis of rotation is between 10 millimeters and 15 millimeters.
• all the fixing means have an identical distance from the axis of rotation. In one embodiment, it is between 10 millimeters and 15 millimeters. This is particularly advantageous, since the washing will be identical from one laboratory container to another.
• the washing device 1 according to the invention has a mass comprised between 1500 grams and 4000 grams, preferably comprised between 2000 grams and 3500 grams, preferably comprised between 2300 grams and 2700 grams.
• the washing device 1 according to the invention has a mass of less than 4000 grams, preferably less than 3000 grams, preferably less than 2500 grams.
• said laboratory container holder 2 has a mass comprised between 10 grams and 1000 grams, preferably comprised between 20 grams and 500 grams, preferably comprised between 200 grams and 300 grams.
• said laboratory container holder 2 has a mass of less than 1000 grams, preferably less than 500 grams, preferably less than 300 grams.
• said stirring means 5 has a mass comprised between 200 grams and 3000 grams, preferably comprised between 500 grams and 2000 grams, preferably comprised between 1000 grams and 1500 grams.
• said stirring means 5 has a mass of less than 3000 grams, preferably less than 2000 grams, preferably less than 1500 grams.
• said base 6 has a mass comprised between 100 grams and 2000 grams, preferably comprised between 400 grams and 1200 grams, preferably comprised between 700 grams and 1000 grams.
• said base 6 has a mass of less than 2000 grams, preferably less than 1000 grams, preferably less than 900 grams.
• the invention also relates to a kit comprising a washing device 1 according to the invention, as well as a plurality of laboratory containers each comprising a hermetic cap.
• said kit preferably comprises at least 2, preferably at least 4, preferably at least 6, preferably at least 8, preferably at least 10, preferably at least 12, preferably at least 14 , preferably at least 16, preferably at least 18, preferably at least 20, preferably at least 22, preferably at least 24, preferably at least 26, preferably at least 28, preferably at least 30, of preferably at least 32, preferably at least 34, preferably at least 36 laboratory containers each comprising a hermetic cap.
• the invention also relates to a washing method using the washing device 1 according to the invention.
• washing device 1 The embodiments described for the washing device 1 are therefore also applicable to the washing process.
• the invention therefore also relates to a method for washing magnetic particles, comprising the following steps:
• step A bringing together said magnetic particles and a washing solution in several laboratory containers;
• step B assembly of the laboratory containers on a laboratory container holder 2;
• steps A, B and C can be done in any order;
• step E dissociation of said laboratory container holder 2 and of said stirring means 5;
• step F mounting of said laboratory container holder 2 on a base 6 adapted to the laboratory container holder 2 comprising at least one magnet able to be reversibly linked to said laboratory container holder 2 and to gather said magnetic laboratory particles within said laboratory containers;
• said laboratory magnetic particles are magnetic beads.
• steps A-G are repeated between 2 and 100 times.
• steps A-G are repeated between 2 and 50 times.
• steps A-G are repeated between 2 and 20 times.
• the laboratory container holder 2 comprising feet 4 as well as 24 means 3 for fixing laboratory containers which are holes with flats arranged on the axes x1 and x2, the stirring means 5 with an axis of rotation y parallel to the axes x1 and x2 when the laboratory container holder 2 is installed on the stirring means 5, as well as the base 6 of the laboratory container holder 2 comprising twelve magnets taken in the mass.
• the base 6 also includes recesses which correspond to the feet of the laboratory container holder 2. When the laboratory container holder 2 is installed on said base 6, the feet 4 of said laboratory container holder 2 fit into the recesses of said base 6.
• FIG. 1 perspective view of the device 1 for washing magnetic laboratory particles according to the invention, with the laboratory container holder 2 mounted on the stirring means 5.
• FIG. 1 perspective view of a laboratory container holder 2 according to the invention comprising 24 fixing means 3 being holes with flats.
• the holes with flats are of different sizes, and therefore suitable for different laboratory containers. It is thus possible to shake laboratory containers of different volumes at the same time.
• FIG. 7 perspective view of two different carrier plates 7, these carrier plates 7 include in particular different fixing means 3, these two carrier plates 7 can be adapted to the same detachable stabilizing base 8.
• Each carrier plate comprises feet 4.
• the base stabilizer comprises fixing means 11 to the stirring means 5 (stirring means 5 not shown).
• the two carrier plates 7 have different colors (not visible in the figure).
• the fixing means 3 are numbered by engraving or printing on said laboratory container holder. The numbers are arranged in such a way that it suffices that said laboratory container holder 2 is turned over by 180° for all the laboratory containers to be easily accessible. In the case where the laboratory container holder 2 is produced by 3D printing, the numbers can thus also be printed.
• Example 1 Use of a device according to the invention in the context of manipulation chromatin immunoprecipitation
• Chromatin immunoprecipitation was performed on 12 samples.
• the chromatin is immunoprecipitated using a specific anti-P65-RELA antibody and a non-specific IgG control antibody.
• the manipulation will therefore involve twice 12 samples, therefore 24 samples.
• the washing device 1 used is that shown in .
• PBS Phosphate Buffered Saline
• step I the use of the device according to the invention allows a single manipulation (of the laboratory container holder) which is used from sub-step 8. and which is placed, during sub-step 9., directly on the stirring medium.
• Step IV Washing the Magnetic Beads
• test tubes remain on the rack from the end of sub-step 1 until sub-step 3.
• step V.1 Add 30 ⁇ l of beads washed and stored at 4°C (step V.1) per tube.
• test tubes remain on the rack from the end of sub-step 1 until sub-step 5.
• Step VIII Elution, reverse crosslink and purification
• Steps Number of manipulations using the device according to the invention Number of manipulations without using the device according to the invention Cold room trips without using the device according to the invention Stage I 1 12 1 Stage II 0 0 1 Stage III 0 0 0 Stage IV 0 0 10 Stage V 2 48 2 Stage VI 3 72 3 Stage VII (if signal/noise too low) 15 (29) 360 (696) 15 (29) Stage VIII 0 0 0 TOTAL (if weak signal/noise) 21 (35) 492 (828) 32 (46) Table 1: Contribution of the use of the device according to the invention

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• Immunology (AREA)
• Chemical & Material Sciences (AREA)
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• Analytical Chemistry (AREA)
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• General Physics & Mathematics (AREA)
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• Engineering & Computer Science (AREA)
• Biomedical Technology (AREA)
• Molecular Biology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Clinical Laboratory Science (AREA)
• Automatic Analysis And Handling Materials Therefor (AREA)
• Cleaning In General (AREA)

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• 2021
  • 2021-09-27 FR FR2110113A patent/FR3127418A1/fr active Pending
• 2022
  • 2022-09-23 EP EP22783041.1A patent/EP4408585A1/de active Pending
  • 2022-09-23 WO PCT/IB2022/059044 patent/WO2023047362A1/fr not_active Ceased

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