EP4365305A3 - Procédé de détection d'un analyte - Google Patents
Procédé de détection d'un analyte Download PDFInfo
- Publication number
- EP4365305A3 EP4365305A3 EP24151623.6A EP24151623A EP4365305A3 EP 4365305 A3 EP4365305 A3 EP 4365305A3 EP 24151623 A EP24151623 A EP 24151623A EP 4365305 A3 EP4365305 A3 EP 4365305A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- detecting
- analyte
- hcr
- rca
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000012491 analyte Substances 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000005096 rolling process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1919029.7A GB201919029D0 (en) | 2019-12-20 | 2019-12-20 | Method of detecting an analyte |
| EP20838034.5A EP4077717B1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
| PCT/EP2020/087185 WO2021123282A1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
Related Parent Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP20838034.5A Division EP4077717B1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
| EP20838034.5A Division-Into EP4077717B1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP4365305A2 EP4365305A2 (fr) | 2024-05-08 |
| EP4365305A3 true EP4365305A3 (fr) | 2024-05-29 |
Family
ID=69322973
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP24151623.6A Pending EP4365305A3 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
| EP20838034.5A Active EP4077717B1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP20838034.5A Active EP4077717B1 (fr) | 2019-12-20 | 2020-12-18 | Procédé de détection d'un analyte |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20210198727A1 (fr) |
| EP (2) | EP4365305A3 (fr) |
| GB (1) | GB201919029D0 (fr) |
| WO (1) | WO2021123282A1 (fr) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3078158A1 (fr) | 2017-10-06 | 2019-04-11 | Cartana Ab | Ligature a matrice d'arn |
| CA3139791A1 (fr) | 2019-05-31 | 2020-12-03 | 10X Genomics, Inc. | Procede de detection de molecules d'acide nucleique cible |
| EP4121561A4 (fr) | 2020-08-06 | 2024-04-17 | Singular Genomics Systems, Inc. | Méthodes pour la transcriptomique et la protéomique in situ |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| EP4347877A1 (fr) | 2021-06-01 | 2024-04-10 | 10X Genomics, Inc. | Procédés et compositions pour la détection d'analytes et la résolution de sondes |
| US20230084407A1 (en) | 2021-06-02 | 2023-03-16 | 10X Genomics, Inc. | Sample analysis using asymmetric circularizable probes |
| WO2023220300A1 (fr) | 2022-05-11 | 2023-11-16 | 10X Genomics, Inc. | Compositions et procédés de séquençage in situ |
| US20240263219A1 (en) * | 2023-01-06 | 2024-08-08 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of variant sequences |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012110899A2 (fr) * | 2011-02-15 | 2012-08-23 | Bernitz Mats Nilsson | Procédé de détection d'arnm in situ |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4883867A (en) | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5340716A (en) | 1991-06-20 | 1994-08-23 | Snytex (U.S.A.) Inc. | Assay method utilizing photoactivated chemiluminescent label |
| ATE152831T1 (de) | 1991-09-16 | 1997-05-15 | Molecular Probes Inc | Dimere unsymmetrische cyaninfarbstoffe |
| US5321130A (en) | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| US5410030A (en) | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5989823A (en) | 1998-09-18 | 1999-11-23 | Nexstar Pharmaceuticals, Inc. | Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
| ATE369439T1 (de) | 1997-12-15 | 2007-08-15 | Csl Behring Gmbh | Markierter primer, geeignet für die detektion von nukleinsäuren |
| DE69940970D1 (de) | 1998-03-25 | 2009-07-23 | Olink Ab | Rolling circle replikation von padlock-sonden |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| SE516272C2 (sv) | 2000-02-18 | 2001-12-10 | Ulf Landegren | Metoder och kit för analytdetektion mha proximitets-probning |
| US6346384B1 (en) | 2000-03-27 | 2002-02-12 | Dade Behring Inc. | Real-time monitoring of PCR using LOCI |
| GB2378245A (en) | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| WO2005019470A2 (fr) | 2003-08-11 | 2005-03-03 | Monogram Biosciences, Inc. | Detection et profilage de complexes moleculaires |
| US20050155478A1 (en) | 2004-01-21 | 2005-07-21 | Ab Sandvik Materials Technology, | Nicked cutting rule |
| WO2005098049A2 (fr) | 2004-03-25 | 2005-10-20 | California Institute Of Technology | Reaction en chaine par hybridation |
| SE0401270D0 (sv) | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| US20060234261A1 (en) | 2005-03-08 | 2006-10-19 | Pierce Niles A | Colorimetric readout of hybridization chain reaction |
| US7721721B1 (en) | 2006-09-28 | 2010-05-25 | Precision Shooting Equipment, Inc. | Reversible and adjustable module system for archery bow |
| GB201413717D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| GB201413718D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| WO2017222453A1 (fr) * | 2016-06-21 | 2017-12-28 | Hauling Thomas | Séquençage d'acide nucléique |
| IL302802A (en) * | 2016-07-05 | 2023-07-01 | California Inst Of Techn | Hybridization chain reaction based on a minimal initiator |
| CN110730825B (zh) * | 2017-05-23 | 2023-08-11 | 新泽西鲁特格斯州立大学 | 用双相互作用发夹探针进行的靶标介导的原位信号放大 |
-
2019
- 2019-12-20 GB GBGB1919029.7A patent/GB201919029D0/en not_active Ceased
-
2020
- 2020-12-18 US US17/127,451 patent/US20210198727A1/en active Pending
- 2020-12-18 EP EP24151623.6A patent/EP4365305A3/fr active Pending
- 2020-12-18 EP EP20838034.5A patent/EP4077717B1/fr active Active
- 2020-12-18 WO PCT/EP2020/087185 patent/WO2021123282A1/fr unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012110899A2 (fr) * | 2011-02-15 | 2012-08-23 | Bernitz Mats Nilsson | Procédé de détection d'arnm in situ |
Non-Patent Citations (3)
| Title |
|---|
| HU XIAOXIA ET AL: "A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe", MIKROCHIMICA ACTA, SPRINGER VERLAG, VIENNA, AT, vol. 186, no. 8, 29 July 2019 (2019-07-29), pages 1 - 6, XP036868548, ISSN: 0026-3672, [retrieved on 20190729], DOI: 10.1007/S00604-019-3666-4 * |
| SAI BI ET AL: "Hybridization chain reaction-based branched rolling circle amplification for chemiluminescence detection of DNA methylation", CHEMICAL COMMUNICATIONS, vol. 49, no. 61, 1 January 2013 (2013-01-01), pages 6906, XP055714735, ISSN: 1359-7345, DOI: 10.1039/c3cc43353f * |
| WEILING SONG ET AL: "Ultrasensitive detection of nucleic acids by template enhanced hybridization followed by rolling circle amplification and catalytic hairpin assembly", CHEMICAL COMMUNICATIONS, vol. 51, no. 12, 1 January 2015 (2015-01-01), pages 2392 - 2395, XP055714776, ISSN: 1359-7345, DOI: 10.1039/C4CC09453K * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4077717C0 (fr) | 2024-02-28 |
| US20210198727A1 (en) | 2021-07-01 |
| EP4365305A2 (fr) | 2024-05-08 |
| WO2021123282A1 (fr) | 2021-06-24 |
| EP4077717B1 (fr) | 2024-02-28 |
| EP4077717A1 (fr) | 2022-10-26 |
| GB201919029D0 (en) | 2020-02-05 |
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Legal Events
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