EP4353817A4 - CELL AGGREGATES MANAGED AT LOW TEMPERATURE AND METHOD FOR MAINTAINING CELL AGGREGATE - Google Patents

CELL AGGREGATES MANAGED AT LOW TEMPERATURE AND METHOD FOR MAINTAINING CELL AGGREGATE

Info

Publication number
EP4353817A4
EP4353817A4 EP22820328.7A EP22820328A EP4353817A4 EP 4353817 A4 EP4353817 A4 EP 4353817A4 EP 22820328 A EP22820328 A EP 22820328A EP 4353817 A4 EP4353817 A4 EP 4353817A4
Authority
EP
European Patent Office
Prior art keywords
cell
maintaining
methods
low
temperature managed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22820328.7A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP4353817A1 (en
Inventor
Kenji Osafune
Shunjiro Sugimoto
Makoto Ryosaka
Yoichi Jimbo
Fumihiko Kitagawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nikkiso Co Ltd
Kyoto University NUC
Original Assignee
Nikkiso Co Ltd
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nikkiso Co Ltd, Kyoto University NUC filed Critical Nikkiso Co Ltd
Publication of EP4353817A1 publication Critical patent/EP4353817A1/en
Publication of EP4353817A4 publication Critical patent/EP4353817A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP22820328.7A 2021-06-11 2022-06-09 CELL AGGREGATES MANAGED AT LOW TEMPERATURE AND METHOD FOR MAINTAINING CELL AGGREGATE Pending EP4353817A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021098281 2021-06-11
PCT/JP2022/023371 WO2022260149A1 (ja) 2021-06-11 2022-06-09 低温管理された細胞凝集塊及び細胞凝集塊の維持方法

Publications (2)

Publication Number Publication Date
EP4353817A1 EP4353817A1 (en) 2024-04-17
EP4353817A4 true EP4353817A4 (en) 2025-07-23

Family

ID=84425246

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22820328.7A Pending EP4353817A4 (en) 2021-06-11 2022-06-09 CELL AGGREGATES MANAGED AT LOW TEMPERATURE AND METHOD FOR MAINTAINING CELL AGGREGATE

Country Status (4)

Country Link
US (1) US20240110157A1 (https=)
EP (1) EP4353817A4 (https=)
JP (1) JPWO2022260149A1 (https=)
WO (1) WO2022260149A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20260117197A1 (en) * 2022-10-07 2026-04-30 Kaneka Corporation Method for preserving and transporting cell aggregate

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3098300A1 (en) * 2014-01-23 2016-11-30 Nissan Chemical Industries, Ltd. Culture medium composition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3633026A4 (en) 2017-05-25 2021-03-10 Kyoto University METHOD FOR INDUCING THE DIFFERENTIATION OF A MESODERMAL INTERMEDIATE CELL TO A KIDNEY PRECURSOR CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF A PLURIPOTENT STEM CELL FROM A KIDNEY PRECURSOR CELL
US20220267736A1 (en) 2017-12-28 2022-08-25 Kaneka Corporation Pluripotent stem cell aggregation suppressor
WO2020242904A1 (en) * 2019-05-30 2020-12-03 Corning Incorporated Cell storage and transportation medium and system and method of transport of cell aggregates
WO2021125340A1 (ja) * 2019-12-19 2021-06-24 国立大学法人京都大学 ネフロン前駆細胞を拡大培養するための培地、ネフロン前駆細胞を拡大培養する方法、腎臓オルガノイドの製造方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3098300A1 (en) * 2014-01-23 2016-11-30 Nissan Chemical Industries, Ltd. Culture medium composition

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAPKUNAS ARVYDAS ET AL: "Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently", SCIENTIFIC REPORTS, vol. 9, no. 1, 17 September 2019 (2019-09-17), US, XP093284907, ISSN: 2045-2322, Retrieved from the Internet <URL:https://www.nature.com/articles/s41598-019-49526-x> DOI: 10.1038/s41598-019-49526-x *
KOSUKE KUSAMORI ET AL: "Development of multicellular spheroid for cell-based therapy", DRUG DELIVERY SYSTEM, vol. 28, no. 1, 1 January 2013 (2013-01-01), pages 45 - 53, XP055558447 *
OSAFUNE ET AL: "Development of iPS cell-based regenerative medicine for kidney diseases", vol. 109, no. 12, 10 December 2020 (2020-12-10), pages 2553 - 2561, XP009541720, ISSN: 0021-5384, Retrieved from the Internet <URL:https://www.jstage.jst.go.jp/article/naika/109/12/109_2553/_article/-char/ja/> DOI: 10.2169/NAIKA.109.2553 *
See also references of WO2022260149A1 *

Also Published As

Publication number Publication date
US20240110157A1 (en) 2024-04-04
WO2022260149A1 (ja) 2022-12-15
EP4353817A1 (en) 2024-04-17
JPWO2022260149A1 (https=) 2022-12-15

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