EP4349966A4 - Verfahren zur herstellung alveolärer organoide unter verwendung induzierter pluripotenter stammzellen aus alveolaren zellen - Google Patents

Verfahren zur herstellung alveolärer organoide unter verwendung induzierter pluripotenter stammzellen aus alveolaren zellen

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Publication number
EP4349966A4
EP4349966A4 EP23789477.9A EP23789477A EP4349966A4 EP 4349966 A4 EP4349966 A4 EP 4349966A4 EP 23789477 A EP23789477 A EP 23789477A EP 4349966 A4 EP4349966 A4 EP 4349966A4
Authority
EP
European Patent Office
Prior art keywords
alveolar
constructing
pluripotent stem
induced pluripotent
organoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP23789477.9A
Other languages
English (en)
French (fr)
Other versions
EP4349966A1 (de
Inventor
Sung Won Kim
Ayoung JO
Min Jae Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Catholic University of Korea
Original Assignee
Industry Academic Cooperation Foundation of Catholic University of Korea
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Academic Cooperation Foundation of Catholic University of Korea filed Critical Industry Academic Cooperation Foundation of Catholic University of Korea
Publication of EP4349966A1 publication Critical patent/EP4349966A1/de
Publication of EP4349966A4 publication Critical patent/EP4349966A4/de
Pending legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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EP23789477.9A 2022-08-26 2023-07-11 Verfahren zur herstellung alveolärer organoide unter verwendung induzierter pluripotenter stammzellen aus alveolaren zellen Pending EP4349966A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020220107869A KR20240029430A (ko) 2022-08-26 2022-08-26 폐포세포 유래 유도만능줄기세포를 이용한 폐포 오가노이드 제작 방법
PCT/KR2023/009885 WO2024043521A1 (ko) 2022-08-26 2023-07-11 폐포세포 유래 유도만능줄기세포를 이용한 폐포 오가노이드 제작 방법

Publications (2)

Publication Number Publication Date
EP4349966A1 EP4349966A1 (de) 2024-04-10
EP4349966A4 true EP4349966A4 (de) 2025-03-05

Family

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EP23789477.9A Pending EP4349966A4 (de) 2022-08-26 2023-07-11 Verfahren zur herstellung alveolärer organoide unter verwendung induzierter pluripotenter stammzellen aus alveolaren zellen

Country Status (4)

Country Link
US (1) US20250092368A1 (de)
EP (1) EP4349966A4 (de)
KR (1) KR20240029430A (de)
WO (1) WO2024043521A1 (de)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938503B2 (en) * 2012-09-25 2018-04-10 Yale University Differentiation of human iPS cells to human alveolar type II via definitive endoderm
US11299712B2 (en) * 2015-03-06 2022-04-12 Kyoto University Method for inducing differentiation of alveolar epithelial cells
CN113215080B (zh) * 2021-05-31 2022-08-16 中国科学院生态环境研究中心 诱导人多能干细胞分化为肺泡细胞或肺泡类器官的培养基及方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102043603B1 (ko) 2012-10-12 2019-11-12 주식회사 차바이오텍 폐암세포의 분리 및 부유배양 기법을 이용한 증식
ES3056357T3 (en) * 2014-01-14 2026-02-19 Univ Yale Compositions and methods of preparing airway cells
US11918702B2 (en) * 2017-03-24 2024-03-05 The Trustees Of Columbia University In The City Of New York Generation of lung bud organoids with branching structures and uses thereof for lung disease modeling
WO2019217429A1 (en) * 2018-05-07 2019-11-14 The Trustees Of Columbia University In The City Of New York Lung and airway progenitors generated from human pluripotent stem cells and related treatments
WO2021021503A1 (en) * 2019-07-26 2021-02-04 The Children's Medical Center Corporation Use of alveolar or airway organoids for the treatment of lung diseases and disorders
US20230138022A1 (en) * 2020-04-01 2023-05-04 National Jewish Health Methods of making pluripotent stem cells and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938503B2 (en) * 2012-09-25 2018-04-10 Yale University Differentiation of human iPS cells to human alveolar type II via definitive endoderm
US11299712B2 (en) * 2015-03-06 2022-04-12 Kyoto University Method for inducing differentiation of alveolar epithelial cells
CN113215080B (zh) * 2021-05-31 2022-08-16 中国科学院生态环境研究中心 诱导人多能干细胞分化为肺泡细胞或肺泡类器官的培养基及方法

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CHEN YA-WEN ET AL: "A three-dimensional model of human lung development and disease from pluripotent stem cells", NATURE CELL BIOLOGY, NATURE PUBLISHING GROUP UK, LONDON, vol. 19, no. 5, 24 April 2017 (2017-04-24), pages 542 - 549, XP037114918, ISSN: 1465-7392, [retrieved on 20170424], DOI: 10.1038/NCB3510 *
GUO LI ET AL: "Interrupted reprogramming of alveolar type II cells induces progenitor-like cells that ameliorate pulmonary fibrosis | npj Regenerative Medicine", NPJ REGENERATIVE MEDICINE, vol. 3, no. 1, 4 September 2018 (2018-09-04), XP093241344, ISSN: 2057-3995, Retrieved from the Internet <URL:https://www.nature.com/articles/s41536-018-0052-5> DOI: 10.1038/s41536-018-0052-5 *
HAN YULING ET AL: "Identification of SARS-CoV-2 inhibitors using lung and colonic organoids", NATURE, vol. 589, no. 7841, 28 October 2020 (2020-10-28), pages 270 - 275, XP037335134, DOI: 10.1038/S41586-020-2901-9 *
MASUI ATSUSHI ET AL: "Perspectives of future lung toxicology studies using human pluripotent stem cells", ARCHIVES OF TOXICOLOGY, SPRINGER, DE, vol. 96, no. 2, 1 January 2022 (2022-01-01), pages 389 - 402, XP037690199, ISSN: 0340-5761, [retrieved on 20220101], DOI: 10.1007/S00204-021-03188-9 *
MILLER ALYSSA J ET AL: "Generation of lung organoids from human pluripotent stem cells in vitro", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 14, no. 2, 21 January 2019 (2019-01-21), pages 518 - 540, XP036687606, ISSN: 1754-2189, [retrieved on 20190121], DOI: 10.1038/S41596-018-0104-8 *
See also references of WO2024043521A1 *
TINDLE COURTNEY ET AL: "Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19", ELIFE, 13 August 2021 (2021-08-13), pages 66417, XP055975259, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463074/pdf/elife-66417.pdf> [retrieved on 20221026] *
YANG WENHAO ET AL: "Human lung organoid: Models for respiratory biology and diseases", DEVELOPMENTAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 494, 5 December 2022 (2022-12-05), pages 26 - 34, XP087251981, ISSN: 0012-1606, [retrieved on 20221205], DOI: 10.1016/J.YDBIO.2022.12.001 *

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Publication number Publication date
KR20240029430A (ko) 2024-03-05
EP4349966A1 (de) 2024-04-10
WO2024043521A1 (ko) 2024-02-29
US20250092368A1 (en) 2025-03-20

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