EP4281586A4 - Emulgierung mit magnetischen hydrogelen - Google Patents

Emulgierung mit magnetischen hydrogelen Download PDF

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Publication number
EP4281586A4
EP4281586A4 EP22743385.1A EP22743385A EP4281586A4 EP 4281586 A4 EP4281586 A4 EP 4281586A4 EP 22743385 A EP22743385 A EP 22743385A EP 4281586 A4 EP4281586 A4 EP 4281586A4
Authority
EP
European Patent Office
Prior art keywords
emulsification
hydrogels
magnetic
magnetic hydrogels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22743385.1A
Other languages
English (en)
French (fr)
Other versions
EP4281586A2 (de
Inventor
Yi XUE
Kristina FONTANEZ
Sepher Kiani
Robert MELTZER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fluent Biosciences Inc
Original Assignee
Fluent Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluent Biosciences Inc filed Critical Fluent Biosciences Inc
Publication of EP4281586A2 publication Critical patent/EP4281586A2/de
Publication of EP4281586A4 publication Critical patent/EP4281586A4/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP22743385.1A 2021-01-25 2022-01-25 Emulgierung mit magnetischen hydrogelen Pending EP4281586A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163141064P 2021-01-25 2021-01-25
PCT/US2022/013720 WO2022159896A2 (en) 2021-01-25 2022-01-25 Emulsification with magnetic hydrogels

Publications (2)

Publication Number Publication Date
EP4281586A2 EP4281586A2 (de) 2023-11-29
EP4281586A4 true EP4281586A4 (de) 2024-12-25

Family

ID=82549920

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22743385.1A Pending EP4281586A4 (de) 2021-01-25 2022-01-25 Emulgierung mit magnetischen hydrogelen

Country Status (3)

Country Link
EP (1) EP4281586A4 (de)
CA (1) CA3209578A1 (de)
WO (1) WO2022159896A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3585364B1 (de) 2017-02-24 2021-04-28 The Regents of the University of California Partikeltropfenstrukturen und verfahren zur herstellung und verwendung davon
KR20240019077A (ko) 2021-04-14 2024-02-14 파틸리온 바이오사이언스 코포레이션 나노규모 반응 챔버 및 이를 사용하는 방법
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
TW202444419A (zh) * 2023-01-31 2024-11-16 美商建南德克公司 磁性水凝膠顆粒及使用方法
WO2025029903A1 (en) * 2023-08-01 2025-02-06 Pierce Biotechnology, Inc. Functionalized magnetic beads and methods of preparation and use

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019076938A1 (en) * 2017-10-16 2019-04-25 Scherr Steven SYSTEMS AND METHODS FOR ENCAPSULATION OF PARTICLES IN MICRO-DARTS
WO2019139650A2 (en) * 2017-09-29 2019-07-18 The Regents Of The University Of California Method of generating monodisperse emulsions
WO2020037214A1 (en) * 2018-08-17 2020-02-20 The Regents Of The University Of California Particle-containing droplet systems with monodisperse fluid volumes
WO2020069298A1 (en) * 2018-09-28 2020-04-02 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US7914994B2 (en) * 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US7183002B2 (en) * 2000-03-24 2007-02-27 Qiagen, Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
CA2575140A1 (en) * 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019139650A2 (en) * 2017-09-29 2019-07-18 The Regents Of The University Of California Method of generating monodisperse emulsions
WO2019076938A1 (en) * 2017-10-16 2019-04-25 Scherr Steven SYSTEMS AND METHODS FOR ENCAPSULATION OF PARTICLES IN MICRO-DARTS
WO2020037214A1 (en) * 2018-08-17 2020-02-20 The Regents Of The University Of California Particle-containing droplet systems with monodisperse fluid volumes
WO2020069298A1 (en) * 2018-09-28 2020-04-02 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
A. ARMSTEAD: "A Novel Hydrogel-Based Approach for Cell Isolation, Purification and Release | American Laboratory", 22 February 2016 (2016-02-22), XP093220113, Retrieved from the Internet <URL:https://www.americanlaboratory.com/914-Application-Notes/183423-A-Novel-Hydrogel-Based-Approach-for-Cell-Isolation-Purification-and-Release/> *
CHEN CHIA-HUNG ET AL: "Microfluidic Assembly of Magnetic Hydrogel Particles with Uniformly Anisotropic Structure", ADVANCED MATERIALS, vol. 21, no. 31, 17 August 2009 (2009-08-17), DE, pages 3201 - 3204, XP093220162, ISSN: 0935-9648, DOI: 10.1002/adma.200900499 *
HATORI MAKIKO N. ET AL: "Particle-Templated Emulsification for Microfluidics-Free Digital Biology", vol. 90, no. 16, 23 July 2018 (2018-07-23), US, pages 9813 - 9820, XP093220159, ISSN: 0003-2700, Retrieved from the Internet <URL:http://pubs.acs.org/doi/pdf/10.1021/acs.analchem.8b01759> DOI: 10.1021/acs.analchem.8b01759 *
HORÁK DANIEL ET AL: "Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics", POLYMER, vol. 46, no. 4, 1 February 2005 (2005-02-01), AMSTERDAM, NL, pages 1245 - 1255, XP093220093, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2004.11.049 *
See also references of WO2022159896A2 *
SONJA BERENSMEIER: "Magnetic particles for the separation and purification of nucleic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 73, no. 3, 25 October 2006 (2006-10-25), pages 495 - 504, XP019458779, ISSN: 1432-0614, DOI: 10.1007/S00253-006-0675-0 *
SU KYUNG SUH ET AL: "Synthesis of magnetic hydrogel microparticles for bioassays and tweezer manipulation in microwells", MICROFLUIDICS AND NANOFLUIDICS, SPRINGER, BERLIN, DE, vol. 13, no. 4, 10 April 2012 (2012-04-10), pages 665 - 674, XP035131175, ISSN: 1613-4990, DOI: 10.1007/S10404-012-0977-8 *
WU CHUEH-YU ET AL: "Monodisperse drops templated by 3D-structured microparticles", SCIENCE ADVANCES, vol. 6, no. 45, 6 November 2020 (2020-11-06), US, XP093220119, ISSN: 2375-2548, DOI: 10.1126/sciadv.abb9023 *

Also Published As

Publication number Publication date
WO2022159896A2 (en) 2022-07-28
EP4281586A2 (de) 2023-11-29
CA3209578A1 (en) 2022-07-28
WO2022159896A3 (en) 2022-09-01

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