WO2022159896A3 - Emulsification with magnetic hydrogels - Google Patents

Emulsification with magnetic hydrogels Download PDF

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Publication number
WO2022159896A3
WO2022159896A3 PCT/US2022/013720 US2022013720W WO2022159896A3 WO 2022159896 A3 WO2022159896 A3 WO 2022159896A3 US 2022013720 W US2022013720 W US 2022013720W WO 2022159896 A3 WO2022159896 A3 WO 2022159896A3
Authority
WO
WIPO (PCT)
Prior art keywords
partitions
hydrogels
analyte
templated
systems
Prior art date
Application number
PCT/US2022/013720
Other languages
French (fr)
Other versions
WO2022159896A2 (en
Inventor
Yi XUE
Kristina FONTANEZ
Sepher Kiani
Robert MELTZER
Original Assignee
Fluent Biosciences Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluent Biosciences Inc. filed Critical Fluent Biosciences Inc.
Priority to CA3209578A priority Critical patent/CA3209578A1/en
Priority to EP22743385.1A priority patent/EP4281586A2/en
Publication of WO2022159896A2 publication Critical patent/WO2022159896A2/en
Publication of WO2022159896A3 publication Critical patent/WO2022159896A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This disclosure relates generally to methods and systems of sample preparation that do not require expensive microfluidic devices or certain time-consuming steps, such as, centrifugation. Instead, systems and methods of the invention use hydrogels that template the formation of partitions inside emulsions and segregate analyte inside the templated partitions. The hydrogels have a hydrogel scaffold embedded with magnetic nanoparticles. The presence of the magnetic nanoparticles provides the ability to interact with and manipulate the templated partitions. Furthermore, the hydrogels include molecular binders that bind with target analyte inside the partitions making the target analyte responsive to magnetic fields for handling analyte during sample preparation.
PCT/US2022/013720 2021-01-25 2022-01-25 Emulsification with magnetic hydrogels WO2022159896A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA3209578A CA3209578A1 (en) 2021-01-25 2022-01-25 Emulsification with magnetic hydrogels
EP22743385.1A EP4281586A2 (en) 2021-01-25 2022-01-25 Emulsification with magnetic hydrogels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163141064P 2021-01-25 2021-01-25
US63/141,064 2021-01-25

Publications (2)

Publication Number Publication Date
WO2022159896A2 WO2022159896A2 (en) 2022-07-28
WO2022159896A3 true WO2022159896A3 (en) 2022-09-01

Family

ID=82549920

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/013720 WO2022159896A2 (en) 2021-01-25 2022-01-25 Emulsification with magnetic hydrogels

Country Status (3)

Country Link
EP (1) EP4281586A2 (en)
CA (1) CA3209578A1 (en)
WO (1) WO2022159896A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1997008547A1 (en) * 1995-08-24 1997-03-06 Theobald Smith Research Institute, Inc. Method and apparatus for isolating nucleic acid
US20060177836A1 (en) * 2004-07-30 2006-08-10 Mckernan Kevin J Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20070080316A1 (en) * 2000-03-24 2007-04-12 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US20120295269A1 (en) * 1998-12-24 2012-11-22 Cepheid Method for separating an analyte from a sample

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1997008547A1 (en) * 1995-08-24 1997-03-06 Theobald Smith Research Institute, Inc. Method and apparatus for isolating nucleic acid
US20120295269A1 (en) * 1998-12-24 2012-11-22 Cepheid Method for separating an analyte from a sample
US20070080316A1 (en) * 2000-03-24 2007-04-12 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US20060177836A1 (en) * 2004-07-30 2006-08-10 Mckernan Kevin J Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERENSME BERENSMEIER SONJAIER: "Magnetic particles for the separation and purification of nucleic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 73, no. 3, 25 October 2006 (2006-10-25), pages 495 - 504, XP037066395 *
SUH ET AL.: "Synthesis of magnetic hydrogel microparticles for bioassays and tweezer manipulation in microwells", MICROFLUIDICS AND NANOFLUIDICS, vol. 13, no. 4, 10 April 2012 (2012-04-10), pages 665 - 674, XP035131175, DOI: 10.1007/s10404-012-0977-8 *

Also Published As

Publication number Publication date
EP4281586A2 (en) 2023-11-29
WO2022159896A2 (en) 2022-07-28
CA3209578A1 (en) 2022-07-28

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