EP4153318A1 - Utilisation de polyamines, de tyramine et/ou d'un extrait vegetal en contenant pour stabiliser des micro-organismes - Google Patents
Utilisation de polyamines, de tyramine et/ou d'un extrait vegetal en contenant pour stabiliser des micro-organismesInfo
- Publication number
- EP4153318A1 EP4153318A1 EP21732467.2A EP21732467A EP4153318A1 EP 4153318 A1 EP4153318 A1 EP 4153318A1 EP 21732467 A EP21732467 A EP 21732467A EP 4153318 A1 EP4153318 A1 EP 4153318A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- bacteria
- composition
- extract
- microorganisms
- spp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/004—Liquid waste from mechanical processing of material, e.g. wash-water, milling fluid, filtrate
Definitions
- the invention relates to the use of an extract of algae and / or plants, said extract containing polyamines and / or tyramine, for stabilizing microorganisms in the face of environmental stress.
- microorganisms contained in the probiotics must remain functional up to their site of action.
- these microorganisms are fragile because they are very sensitive to environmental stresses, for example variations in pH.
- silage namely the conservation of fodder by lactic fermentation
- the microorganisms are also subjected to a certain number of environmental stresses such as oxidative stress, osmotic or saline stress, thermal stress, acid stress. - basic and / or stress linked to competing microorganisms.
- the microorganisms used in animal feed are therefore exposed to the same types of stress as those encountered in agriculture, which therefore prevents their growth, their stability and / or their effects.
- the present invention which finds application in the agro-ecological and agricultural field, aims to provide the use of polyamines, tyramine and / or a plant extract containing it to stabilize microorganisms in the face of environmental stress, such as oxidative stress, osmotic stress or salt stress, heat stress, acid-base stress and / or stress linked to competing microorganisms.
- environmental stress such as oxidative stress, osmotic stress or salt stress, heat stress, acid-base stress and / or stress linked to competing microorganisms.
- the invention relates to the use of an extract of algae and / or plants, said extract containing polyamines and / or tyramine, for stabilizing microorganisms in the face of stress. environmental.
- the invention relates to a composition
- a composition comprising:
- microorganisms chosen from: (i) bacteria fixing atmospheric nitrogen, such as Azotobacter or Azospirillum (ii) rhizobacteria promoting plant growth (PGPR or Plant Growth Promoting Rhizobacteria), (iii) phosphorus solubilizing bacteria such as BaciHus amyloliquefaciens, (iv) root protection bacteria (PGPR) capable of opposing the activity of pathogens such as BaciHus subti / is or Pseudomonas spp., (v) bacteria producing phytohormones such as BaciHus amyloliquefaciens or BaciHus radicoia, (vi) bacteria involved in the process of mineralization of organic matter such as LactobaciHus rhamnosus or LactobaciHus faciminis, (vii) iron solubilizing bacteria such as Pseudomonas spp., (viii) silica solubilizing bacteria, (ix) sulfur oxidizing bacteria, (
- the invention relates to a method for fertilizing a plant characterized in that it comprises supplying said plant with a composition according to the invention.
- Tyramine denotes a chemical compound of monoamine type, the CAS number of which is 51-67-2. Tyramine has the following formula:
- polyamines denotes organic compounds containing at least two amine functions. Polyamines are found in almost all plants, animals and microorganisms. Polyamines are involved in a variety of cellular processes such as the expression of certain genes, cell growth, cell survival and cell proliferation.
- the polyamines can be chosen from cadaverine, spermidine, spermine and / or putrescine.
- the plant extract used in the context of the invention can therefore comprise one, two, three or all four polyamines chosen from cadaverine, spermidine, spermine and putrescine.
- Cadaverine or pentamethylenediamine
- the CAS number of which is 462-94-2 has the following formula:
- Putrescine (or tetramethylene diamine), the CAS number of which is 110-60-1, has the following formula:
- plant extract denotes the product resulting from an extraction of the contents of the cells of a plant.
- the plant extract contains an effective amount of polyamines and / or tyramine.
- the plant extract can be an extract of algae and / or plants.
- the term "extract of algae and / or plants” denotes the product resulting from an extraction of the contents of the cells of algae and / or plants respectively.
- the algae extract can be an extract of brown algae, such as an extract of brown algae of the Fucaceae family.
- the extract of brown algae can be an extract of Fucus vesicu / osus or Ascophyllum noc / osum.
- the plant extract can be an extract of beet molasses vinasse.
- the preparation of a plant extract containing an effective amount of polyamines and / or tyramine does not present any particular difficulty, many extraction processes are described in the literature.
- the extraction process is not limited to any particular process, and conventional processes can be used to prepare such an extract.
- it can be obtained by a process comprising the following steps: washing, grinding, extraction with a solvent (eg water), solid / liquid separation and optionally fractionation and / or concentration.
- a solvent eg water
- the plant extract obtained can be more or less concentrated depending on the use envisaged, for example it is possible to concentrate the extract by an evaporation technique. Total dehydration of this extract allowing presentation in water-soluble powder form can be obtained, for example, by tumble dryer or by atomization.
- the extraction conditions and the nature of the plant will be chosen such that the extract obtained exhibits the quantity of polyamines and / or tyramine desired in the envisaged application.
- the extract when it is an extract of beet molasses vinasse, it can be obtained by a process comprising the following steps: washing of the beets, grinding of the beets, extraction of the crystallizable sugar from the beet, obtaining two by-products: molasses and beet pulp, recovery of molasses, fermentation of molasses to obtain molasses stillage and concentration [2] [3]. It is also possible to enrich the plant extract with polyamines and / or tyramine, for example by an ultrafiltration technique.
- the term "effective amount of polyamines and / or tyramine” denotes the amount of polyamines and / or tyramine sufficient to stabilize the microorganisms in the face of environmental stress.
- the plant extract preferably contains at least 0.01% by mass of polyamines and / or tyramine relative to the total dry mass of the extract (% w / w), for example at least 0.05% w / w, at least 0.5% w / w, at least 0.2% w / w, for example between 0.01% w / w and 1% w / w, between 0.05% w / w and 0, 5% w / w.
- the assay of the amount of polyamines and tyramines in the extract does not present any particular difficulty, assay methods being described in the literature, such as for example the HPLC method [1].
- the plant extract can be more or less concentrated in polyamines and / or in tyramine depending on the use envisaged.
- microorganisms designates microscopic organisms such as bacteria, microscopic fungi, for example filamentous microscopic fungi, yeasts.
- the microorganisms can be chosen from (i) bacteria fixing atmospheric nitrogen, such as Azotobacter or Azospirillum (ii) rhizobacteria promoting plant growth (PGPR or Plant Growth
- Rhizobacteria Promoting Rhizobacteria
- phosphorus solubilizing bacteria such as BaciHus amyloliquefaciens
- plant protection bacteria PGPR
- bacteria producing phytohormones such as BaciHus amyloliquefaciens or BaciHus radicoia
- bacteria involved in the mineralization process of organic matter such as LactobaciHus rhamnosus or LactobaciHus faciminis
- iron solubilizing bacteria such as Pseudomonas spp.
- silica solubilizing bacteria sulfur oxidizing bacteria
- lactic acid bacteria such as LactobaciHus spp., Lactococcus spp.
- lactic acid bacteria such as LactobaciHus spp., Lactococcus spp.
- Bifidobacterium spp. (Xi) bacteria of the genus Enterococcus spp., (Xii) bacteria of the genus Pediococcus spp., (Xiii) bacteria of the genus BaciHus Hcheniformis, (xiv) mycorrhizal fungi such as Rhizophagus irregularis, (xv) yeasts of the genus Saccharomyces cerevisiae and (xvi) a mixture of at least two microorganisms chosen from (i) to (xv).
- composition denotes a mixture of one or more substances distinct from one another.
- a composition may be a fertilizing composition, a composition intended for animal feed or a composition intended for the preservation of silage.
- a “fertilizing composition” denotes a composition comprising a fertilizing substance, or a mixture of fertilizing substances, natural or of synthetic origin, used in agriculture, horticulture and forestry.
- fertilizer means a fertilizer and / or an amendment.
- fertilizer designates fertilizing materials whose main function is to provide the plants with elements directly useful for their nutrition (major fertilizing elements, secondary fertilizing elements and trace elements).
- amendment denotes a substance intended to improve the quality of soils, and in particular intended to improve the pH of the soils.
- the amendment is chosen from basic mineral amendments of limestone and / or limestone and magnesium type; humus amendments such as composts or manure.
- composition intended for animal feed can comprise substances intended to improve the performance and / or the health of the animals.
- a composition intended for animal feed can, for example, comprise probiotic foods, mineral-vitamin supplements, etc.
- composition intended for the preservation of silage can comprise substances intended to improve the preservation and the quality of the silages.
- a composition intended for silage preservation can, for example, include lactose, xylanase or beta-glucanase enzymes, etc.
- plant is intended in the present application to denote the plant considered as a whole, including its root system, its vegetative system, seeds, seeds and fruits.
- the present invention results from the surprising advantages demonstrated by the inventors of the effect of polyamines, of tyramine and / or of a plant extract containing it to stabilize microorganisms.
- the present application relates to the use of polyamines, tyramine and / or a plant extract containing it to stabilize microorganisms in the face of environmental stress, such as oxidative stress, stress. osmotic or saline stress, thermal stress, acid-base stress and / or stress linked to competing microorganisms.
- the first object of the invention relates to the use of an extract of algae and / or plants, said extract containing polyamines and / or tyramine, for stabilizing microorganisms in the face of environmental stress. , such as oxidative stress, osmotic stress or salt stress, heat stress, acid-base stress and / or stress linked to competing microorganisms.
- the plant extract contains an effective amount of polyamines and / or tyramine.
- the plant extract is an extract of brown algae, for example an extract of brown algae of the Fucaceae family, and / or an extract of beet molasses vinasse. It is understood that the plant used for the preparation of the plant extract naturally comprises polyamines and / or tyramine. Consequently, the polyamines and / or the tyramine contained in the extract of algae and / or plants come directly from the algae and / or plants used to prepare said extract.
- Oxidative stress corresponds to an attack on microorganisms by free radicals, also called "reactive oxygen species" (ROS).
- ROS reactive oxygen species
- An acid-base stress corresponds to a stress linked to the pH. Indeed, it is known that microorganisms are more or less stable at certain pHs, for example a pH ranging from 1 to 12, for example ranging from 3 to 9, for example a pH 5 or for example a pH 9.
- Osmotic stress corresponds to stress linked to osmolarity, ie to the salt content in the medium. In fact, it is known that microorganisms are more or less stable at certain osmolarities, for example at a salt concentration greater than 0.3 M, microorganisms are generally less stable.
- Water stress corresponds to stress linked to the absence of water, ie to the water content in the medium.
- Thermal stress corresponds to stress induced by cold or by heat. Depending on the microorganism considered, the temperature inducing thermal stress varies. Stress linked to competing microorganisms corresponds to stress linked to the presence of microorganisms other than microorganisms stabilized with the plant extract. It may be, for example, micro ⁇ organisms naturally present in the soil that will compete for space in the ground and for access to nutrients with microorganisms added to the soil.
- the competing microorganisms are bacteria of the genus Agrobacterium spp., Of the genus Erwinia spp., Or of the genus Xanthomonas spp., Or fungi of the genus Fusarium spp.
- stabilization consists in increasing the survival rate of microorganisms, in particular measured after 24 hours, 48 hours or 72 hours of growth, under conditions of environmental stress compared to the same conditions without polyamines, tyramine and / or plant extract containing it.
- the survival rate of microorganisms with polyamines, tyramine and / or the plant extract containing them, and subjected to environmental stress is advantageously at least 1 log, at least 2 log, at least 3 log, at least 4 log, at least 5 log greater than the survival rate of the same microorganisms subjected to the same environmental stress but without polyamines, tyramine and / or plant extract containing it.
- the survival rate is measured by comparing the quantity of bacteria (for example in CFU) after a given time, for example 24 hours, 48 hours or 72 hours.
- the microorganisms are contained in a composition, such as a fertilizing composition, a composition intended for animal feed or a composition intended for the preservation of silage.
- the microorganisms may be contained in said composition in an amount ranging from 10 2 to 10 50 CFU per tonne of composition, preferably ranging from 10 5 to 10 20 CFU per tonne of composition, preferably approximately 10 11 CFU per tonne of composition.
- Polyamines, tyramine and / or the plant extract containing it can therefore be used as a complement in a composition, for example a fertilizing composition, a composition intended for animal feed or a composition intended for the preservation of silage, to stabilize the microorganisms contained in these compositions.
- the present application relates to a composition
- a composition comprising:
- microorganisms chosen from: (i) bacteria fixing atmospheric nitrogen, such as Azotobacter or Azospirillum (ii) rhizobacteria promoting plant growth (PGPR or Plant Growth Promoting Rhizobacteria), (iii) phosphorus solubilizing bacteria such as BaciHus amyloliquefaciens, (iv) plant protection bacteria (PGPR) capable of opposing the activity of pathogens such as BaciHus subti / is or Pseudomonas spp., (v) producing bacteria phytohormones such as BaciHus amyloliquefaciens or BaciHus radicoia, (vi) bacteria involved in the mineralization process of organic matter such as LactobaciHus rhamnosus or LactobaciHus faciminis, (vii) iron solubilizing bacteria such as Pseudomonas spp., (viii) silica solubilizing bacteria, (ix) sulfur oxidizing bacteria,
- a second object of the invention relates to a composition
- a composition comprising: (a) an extract of algae and / or plants, said extract containing polyamines and / or tyramine, and (b) selected microorganisms among: (i) atmospheric nitrogen fixing bacteria, such as Azotobacter or Azospirillum (ii) rhizobacteria promoting plant growth (PGPR or Plant Growth Promoting Rhizobacteria), (iii) phosphorus solubilizing bacteria such as BaciHus amyloliquefaciens , (iv) plant protection bacteria of the roots (PGPR) capable of opposing the activity of pathogenic agents such as BaciHus subti / is or Pseudomonas spp., (v) bacteria producing phytohormones such as BaciHus amyloliquefaciens or BaciHus radicoia, (vi) bacteria involved in the mineralization process of organic matter such as LactobaciHus rhamnosus or
- yeasts of the genus Saccharomyces cerevisiae and (xvi) a mixture of at least two microorganisms chosen from (i) to (xv).
- (a) is a plant extract containing an effective amount of polyamines and / or tyramine.
- the plant extract is an extract of algae and / or plants, preferably an extract of brown algae, for example an extract of brown algae of the Fucaceae family, and / or an extract of beet molasses stillage.
- the plant used for the preparation of (a) the plant extract naturally comprises polyamines and / or tyramine. Consequently, the polyamines and / or the tyramine contained in (a) the extract of algae and / or plants come directly from the algae and / or plants used to prepare said extract.
- the composition according to the invention may in particular comprise from 10 2 to 10 50 CFU of microorganisms (b) per tonne of composition, preferably from 10 5 to 10 20 CFU of microorganisms (b) per tonne of composition, preferably 10 11 CFU of microorganisms (b) per tonne of the composition.
- the composition may in particular comprise from 0.1 to 100 grams of polyamines and / or tyramine per tonne of composition, preferably from 0.4 to 10 grams of polyamines and / or tyramine per tonne of composition.
- the composition comprises: - from 0.1 to 100 grams of polyamines and / or tyramine per tonne of composition, preferably from 0.4 to 10 grams of polyamines and / or tyramine per tonne of composition, and
- composition according to the invention can also comprise (c) a fertilizer and / or an amendment.
- the fertilizer can be one or more active substances chosen from nitrogen, phosphorus, potassium, urea, ammonium sulfate, ammonium nitrate, phosphate, potassium chloride, ammonium sulfate, magnesium nitrate, manganese nitrate, zinc nitrate, copper nitrate, phosphoric acid, potassium nitrate, boric acid and their mixtures, preferably a mixture of nitrogen , potassium and phosphorus or a mixture of phosphorus and potassium.
- the amendment can be one or several active substances chosen from basic mineral amendments of the limestone type, basic mineral amendments of the magnesium type, humus amendments of the composts type and / or humus amendments of the manure type, preferably fertilizers and mineral amendments.
- Polyamines, tyramine and / or the plant extract containing them preferably the plant extract (a) containing an effective amount of polyamines and / or tyramine, for example the algae extract and / or of plants (a), preferably the extract of algae and / or plants (a) containing an effective amount of polyamines and / or tyramine, stabilize the microorganisms (b) in the face of environmental stress, such as oxidative stress, osmotic stress or salt stress, heat stress, acid-base stress and / or stress linked to competing microorganisms.
- environmental stress such as oxidative stress, osmotic stress or salt stress, heat stress, acid-base stress and / or stress linked to competing microorganisms.
- the microorganisms (b) can be sensitive and / or unstable in the presence of a fertilizer, at a pH ranging from 1 to 12, preferably at a pH ranging from 3 to 9, for example at a pH of '' about 5 or at a pH of about 9 and / or at a salinity going beyond the threshold of sensitivity / stability to the salt of a microorganism in question, for example at a salt concentration greater than 0.3 M for bacteria of the genus Azobacter chroococcum.
- the composition further comprises a fertilizer and has a pH ranging from 1 to 12, preferably a pH ranging from 3 to 9, for example a pH of about 5 or a pH of approximately 9 and / or a salinity going beyond the threshold of sensitivity / stability to the salt of a microorganism in question, for example at a salt concentration greater than 0.3 M for bacteria of the genus Azobacter chroococcum.
- the composition according to the invention can be in solid or liquid form, preferably in solid form.
- composition according to the invention can also comprise other substances, such as biostimulant substances, phytohormones, polyphenols, amino acids, etc.
- the composition according to the invention may be a fertilizing composition, a composition intended for animal feed or a composition intended for the preservation of silage. The other substances will therefore be chosen according to the intended use.
- compositions comprising limestone amendments, organic amendments and growing media, root fertilizers of NP, PK type,
- NPK foliar fertilizers and / or biostimulants or root nutrient solutions.
- compositions intended for animal feed according to the invention mention will be made of compositions comprising, for example, sodium bicarbonate, magnesium oxide, nutritional yeast.
- compositions intended for the preservation of silage according to the invention, mention will be made of compositions comprising, for example, lactose, enzymes of xylanase, beta-glucanase, etc. type.
- a third object of the invention relates to a method for fertilizing a plant characterized in that it comprises supplying said plant with a composition according to the invention.
- the method according to the invention makes it possible to stimulate the development of a plant and / or to stimulate the yield of a plant.
- the composition can be added to said plant in an amount ranging from 0.5 to 1500 kg / ha, preferably ranging from 1 to 1000 kg / ha kg / ha.
- the composition can be provided in solid or liquid form. According to the process of the invention, the composition can advantageously be a fertilizing composition, such as that described above.
- the application to said plant will be carried out by foliar route or by root route, preferably by root route.
- the present invention finds application in the treatment of a very wide variety of plants.
- - field crops such as cereals (wheat, corn, sugar cane, etc.), - protein crops (peas),
- the plant belongs to the order of monocotyledons, preferably to the Poaceae family, preferably the plant is chosen from wheat, rice, barley, l oats, rye, meadow, sugar cane or corn.
- Example 1 Preparation of an extract of alaues and / or plants
- Example 1 a) Preparation of an extract of brown algae of the Fucaceae family of the genus Ascophyllum nodosum 200 g of fresh algae of the genus Ascophyllum nodosum were ground to obtain fragments of fresh algae having a size of about 5 mm.
- the aqueous extraction of fresh algae was carried out using 200 g of Ascophyllum nodosum per liter of water.
- the extraction was carried out in a 0.5N solution of sulfuric acid with stirring which lasts 2 hours at room temperature.
- the extract was then filtered through a membrane (80 ⁇ m porosity) and then concentrated on a falling flow evaporator.
- the analysis of one kilo of extract was carried out by High Performance Liquid Chromatography.
- the analytical method used for the identification and Quantification of polyamines is based on the method described by HB Papenfus (2011).
- Table 1 One kilogram of dry extract (Table 1) contains 3300 mg of polyamines in total, ie 480 mg of putrescine, 1470 mg of cadaverine, 840 mg of spermidine and 510 mg of spermine.
- the aqueous extraction of fresh algae was carried out using 200 g of Fucus vesicu / osus per liter of water.
- the extraction was carried out in a 1N solution of sulfuric acid with stirring which lasts 2 hours at room temperature.
- the extract was then filtered through a membrane (80 ⁇ m porosity) and then concentrated on a falling flow evaporator.
- the analysis of one kilo of extract was carried out by High Performance Liquid Chromatography.
- the analytical method used for the identification and quantification of polyamines is based on the method described by H. B. Papenfus (2011).
- Table 2 One kilo of dry extract (Table 2) contains 7170 mg of polyamines in total: 1140 mg of putrescine, 150 mg of cadaverine, 1830 mg of spermidine and 4050 mg of spermine. Table 2
- An extract of beet molasses vinasse suitable for the implementation of the invention is available under the following trade names: Proteinal, Sirional, Betainex, Viprotal, Vinasse FS, Citrocol and Prodyn [3].
- One kilogram of dry extract (Table 3) contains 477 mg of polyamines in total: 344 mg of tyramine, 73 mg of putrescine, 48 mg of cadaverine and 12 mg of spermidine.
- the starting inoculum for each of the strains was calibrated by measuring the OD (620 nm) in order to reach an initial concentration around 10 3 -10 4 CFU / mL.
- the strains were incubated for 24 to 72 h.
- Counts were carried out in TSA (Tryptone Soya Agar) agar at T0 and after 24 h, 48 h or 72 h of incubation at 30 ° C (+/- 1 ° C), depending on the strains studied.
- survival rates in the presence or absence of the extract and / or stress were calculated relative to the bacterial concentration obtained at the end of kinetics in the modality containing the strain alone under stress conditions without addition of the extract.
- Example 2 b Effect of the extract of Fucus vesicu / osus prepared according to example 1 a] on the growth of Bacillus Hcheniformis under acid stress condition
- the addition of 0.4 M NaCl in the culture medium made it possible to reach a limiting level of salt stress.
- the addition of the Ascophyllum nodosum extract made it possible to increase the bacterial concentration by 1.5 logs after 72 h of incubation.
- the bacterial concentration reached is 2.6 logs higher than that of the strain control alone but remains lower than that of the salt-free condition with the addition of the Ascophyllum nodosum extract.
- Example 2 d Effect of the extract ô'Ascophyllum nodosum prepared according to example la] on the growth of ô'Azospirillum brasilense under heat stress conditions
- the Azospirillum brasilense strain was tested under heat stress conditions, that is to say by increasing the temperature of the culture medium from
- the incubation of A brasiiense 11 h at 45 ° C made it possible to reproduce the conditions of thermal stress with a loss of 5.9 logs of the bacterial concentration after 72 h of incubation compared to the condition at 30 ° C. Under these conditions, the addition of the Ascophyllum nodosum extract allowed an increase of 5.5 logs in the bacterial concentration after 72 h of incubation.
- the bacterial concentration reached is globally similar to that of the control at 30 ° C, around 5.10 6 CFU / mL.
- Example 2 fi Effect of the extract of beet molasses stillage prepared according to example 1c on the growth of AzosDirillum brasilense under conditions of basic stress
- Example 2 cO Effect of the extract of beet molasses vinasse prepared according to example 1c on the growth of a complex of nitrogen-fixing rhizobacteria (Azotobacter diazotrophicus. Paenibacillus azotofixans.
- Glycine betaine known as an osmoprotector, was tested in comparison with the extract ô'Ascophyllum nodosum prepared according to example la).
- Polyamines make it possible to stabilize Azotobacter chroococcum in the face of osmotic stress, whereas no stabilization is observed with glycine betaine under conditions of osmotic stress.
- Example 3 Effect of a biofertilizer composition based on an extract of Ascoohyllum nodosum on the growth of tomatoes grown in the open field
- the test was carried out on a crop of tomatoes of the species So / anum lycopersicum.
- the experimental set-up includes modalities with 5 repetitions.
- the Ascophyllum nodosum extract was incorporated in an amount of lg / T in a solution of PK (5-30), which was enriched with the strain Bacillus Hcheniformis.
- the following treatments are applied at the 3 leaf stage of tomato plants: - a control not including fertilizer,
- the PK 5-30 + B. Hcheniformis solution slightly improved the yield, ie 5.2% compared to the control.
- the use of the extract in combination with the PK 5-30 + bacteria solution allowed an improvement in the yield compared to the control of more than 14.8%. Compared to the solution
- Example 4 Biofertilizing compositions or compositions intended for animal feed according to the invention [0127]
- Example 4 c root fertilizers
- Table 16 below describes the composition of an N P type root fertilizer.
- Table 17 describes the composition of a root fertilizer of NPK and magnesium oxide type.
- Table 18 below describes the composition of a solution of NPK and magnesium type.
- Table 19 describes the composition of a solution of N, Calcium, Magnesium type.
- Example 4 e Compositions intended for animal feed
- Table 20 below describes the composition of a probiotic.
- Table 20 Table 21 below describes the composition of a silage preservative.
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Abstract
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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FR2005016A FR3110597B1 (fr) | 2020-05-19 | 2020-05-19 | Utilisation de polyamines, de tyramine et/ou d’un extrait végétal en contenant pour stabiliser des micro-organismes. |
PCT/FR2021/050855 WO2021234262A1 (fr) | 2020-05-19 | 2021-05-18 | Utilisation de polyamines, de tyramine et/ou d'un extrait vegetal en contenant pour stabiliser des micro-organismes |
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EP4153318A1 true EP4153318A1 (fr) | 2023-03-29 |
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Application Number | Title | Priority Date | Filing Date |
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EP21732467.2A Pending EP4153318A1 (fr) | 2020-05-19 | 2021-05-18 | Utilisation de polyamines, de tyramine et/ou d'un extrait vegetal en contenant pour stabiliser des micro-organismes |
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US (1) | US20230234897A1 (fr) |
EP (1) | EP4153318A1 (fr) |
BR (1) | BR112022022621A2 (fr) |
CA (1) | CA3177812A1 (fr) |
FR (1) | FR3110597B1 (fr) |
WO (1) | WO2021234262A1 (fr) |
ZA (1) | ZA202212644B (fr) |
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JP2015030688A (ja) * | 2013-08-01 | 2015-02-16 | 興人ライフサイエンス株式会社 | 栄養組成物 |
WO2018182555A2 (fr) * | 2016-10-05 | 2018-10-04 | Yedi̇tepe Sağlik Hi̇zmetleri̇ Anoni̇m Şi̇rketi̇ | Micro-organismes efficaces pour prévenir le stress induit par le froid chez les plantes |
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2020
- 2020-05-19 FR FR2005016A patent/FR3110597B1/fr active Active
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2021
- 2021-05-18 WO PCT/FR2021/050855 patent/WO2021234262A1/fr unknown
- 2021-05-18 CA CA3177812A patent/CA3177812A1/fr active Pending
- 2021-05-18 BR BR112022022621A patent/BR112022022621A2/pt unknown
- 2021-05-18 US US17/926,506 patent/US20230234897A1/en active Pending
- 2021-05-18 EP EP21732467.2A patent/EP4153318A1/fr active Pending
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2022
- 2022-11-21 ZA ZA2022/12644A patent/ZA202212644B/en unknown
Also Published As
Publication number | Publication date |
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BR112022022621A2 (pt) | 2022-12-20 |
US20230234897A1 (en) | 2023-07-27 |
FR3110597A1 (fr) | 2021-11-26 |
WO2021234262A1 (fr) | 2021-11-25 |
FR3110597B1 (fr) | 2022-12-16 |
ZA202212644B (en) | 2023-10-25 |
CA3177812A1 (fr) | 2021-11-25 |
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