EP4061514A1 - Appareil de filtrage - Google Patents

Appareil de filtrage

Info

Publication number
EP4061514A1
EP4061514A1 EP20811255.7A EP20811255A EP4061514A1 EP 4061514 A1 EP4061514 A1 EP 4061514A1 EP 20811255 A EP20811255 A EP 20811255A EP 4061514 A1 EP4061514 A1 EP 4061514A1
Authority
EP
European Patent Office
Prior art keywords
pump
filtration device
filter module
centrifugal pump
adapter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20811255.7A
Other languages
German (de)
English (en)
Inventor
Dejan Nikolic
Alexander Heide
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unicyte EV AG
Fresenius Medical Care Deutschland GmbH
Original Assignee
Unicyte EV AG
Fresenius Medical Care Deutschland GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unicyte EV AG, Fresenius Medical Care Deutschland GmbH filed Critical Unicyte EV AG
Publication of EP4061514A1 publication Critical patent/EP4061514A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/02—Hollow fibre modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/10—Specific supply elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/24—Specific pressurizing or depressurizing means
    • B01D2313/243—Pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/10—Cross-flow filtration

Definitions

  • the present invention relates to a filtration device for filtering liquids with a filter module and with a pump, the pump being arranged in such a way that it conveys the liquid into the filter module.
  • TFF tangential flow filtration
  • Such a TFF method which is the subject of the present invention in a preferred embodiment, is known, for example, from WO 2017/117585 A1.
  • this outlet must also be oriented vertically if a centrifugal pump with a tan gential outlet is directly connected.
  • centrifugal rotor pumps generally have a housing with an essentially cylindrical cavity in which the pump rotor moves and through which the medium to be pumped is moved. If a tangential outlet is to be oriented vertically, it cannot be attached to the highest point of the housing - then it would be a radial outlet.
  • the present invention is therefore based on the object of developing a filtration device of the type mentioned at the outset in such a way that the accumulation of air within the pump is reliably prevented even at lower speeds of the impeller.
  • the filter module is arranged vertically and that the pump is a centrifugal pump which has a tangential outlet which forms the highest point of the centrifugal pump.
  • the vertical arrangement of the filter module according to the invention ensures the most uniform possible filtration.
  • the arrangement or rotation of the centrifugal pump from a vertical position of the outlet to a position in which the tangential outlet forms the highest point on the housing of the pump chamber has the advantage that the air bubbles upwards out of the without further action due to gravity escape through the tangential outlet, which is not possible if the outlet does not form the highest point of the pump or the pump housing.
  • the “highest point” of the pump is understood to mean the highest point of the delivery area of the pump, ie the highest point of the area in which the impeller is located, preferably the pump head. In other words, it is the highest point of the housing of the pump chamber to which the outlet is attached.
  • the "vertical arrangement” also includes cases in which the filter module is essentially vertical, e.g. at an angle of ⁇ 10 ° to 15 ° relative to the vertical.
  • the tangential outlet of the pump is in an inclined position, i.e. not pointing vertically upwards or horizontally to the side.
  • a hose section or a pipe socket which is preferably an integral part of the centrifugal pump, is preferably connected to the outlet.
  • Other connection elements that connect to the outlet are also included in the invention.
  • the hose piece or the pipe socket extends relative to the horizontal at an angle between 35 ° and 55 °, preferably at an angle between 40 ° and 50 °, i.e. neither vertically nor horizontally.
  • an adapter is located between the centrifugal pump and the filter module.
  • the adapter is curved or angled.
  • the adapter preferably connects the hose section or the pipe section to the inlet of the filter module, for example by means of flange connections. It is also conceivable that the adapter is arranged directly at the outlet of the pump.
  • connection in particular Luer lock connections
  • Luer lock connections are arranged on the adapter. These can be used, for example, to arrange one or more sensors and / or sampling points on the adapter without a flange connection of the adapter being necessary for this.
  • the filter module is a hollow fiber module, as is known, for example, from the field of hemodialysis.
  • the present invention also relates to a filtration device for filtering liquids with a filter module and with a pump, the pump being arranged such that it conveys the liquid into the filter module, the pump being a centrifugal pump, which has a tangential Has outlet which forms the highest point of the centrifugal pump and that there is a curved or angled adapter between the centrifugal pump and the filter module.
  • the filtration device is designed according to one of claims 1 to 9.
  • the present invention also relates to a method for purifying substances, such as extracellular vesicles, from a biological fluid by means of a TFF purification process, the biological fluid being conveyed by means of a centrifugal pump.
  • a peristaltic pump is that no significant pressure pulses are generated that could damage sensitive components of the fluid, such as extracellular vesicles, for example.
  • the centrifugal pump rotor is preferably an impeller.
  • the centrifugal pump is equipped with a radially pumping impeller and this impeller is designed as an impeller.
  • the impeller is also called the impeller of the pump.
  • An impeller can preferably be designed with a hollow center.
  • such an impeller can be essentially disk-shaped and have essentially radially extending impeller blades which entrain a pump medium to be conveyed when the pump wheel rotates. That is, a first disk forms a base on which airfoils are arranged. In other words, the blades are on the first disk.
  • the end of the impeller blades remote from the first disk plane can in turn be connected to a further disk arranged parallel to the first disk.
  • a second disk can connect the ends of the blades opposite the first disk, so to speak form a closure.
  • the second disk is then parallel to the first disk and the blades lie between the two disks.
  • An embodiment with two disks arranged parallel to one another is just as conceivable as an embodiment with only one disk on which the impeller blades are arranged while the end of the impeller blades remote from the disc is free.
  • a first variant of such a disk has a central recess, that is, roughly speaking, is ring-shaped.
  • An alternative variant of such a disc is designed as a circular disc, so it just has no central recess.
  • an impeller with a hollow center is to be understood as meaning that the impeller blades do not extend towards the center as far as the axis of rotation, but that a macroscopically recognizable area on and / or immediately around the axis of rotation of the impeller is free of blades is.
  • the hollow center is also not filled with a solid solid, such as a solid cylinder. Rather, the center is hollow so that the liquid to be pumped can flow through the hollow center. It is conceivable that a solid body lies exactly on the axis of rotation of the pump wheel, for example an axis or a shaft. Then the hollow center is the area located centrally around the axis of rotation between the solid body and the blades - i.e. the area through which the liquid to be pumped can flow freely.
  • the hollow center can then be designed, for example, essentially like a cylinder jacket with a finite jacket thickness.
  • the housing of the filter module has an internal support plate with openings which are arranged between the centrifugal pump rotor and the filter bundle so that liquid flows from the centrifugal pump rotor through the openings and then to the fibers of the filter when the centrifugal pump rotor is rotating is offset.
  • the impeller is magnetically levitated.
  • Examples of a magnetically floating impeller pump are described, inter alia, in EP1930034.
  • the biological fluid to be cleaned or processed is, for example, cell culture supernatants, blood components such as blood serum or plasma or urine.
  • the biological materials to be purified are biological macromolecules and biological microstructures, in particular antibodies, antibody conjugates, antibody fragment conjugates, virus particles, ribonucleic acid (short RNA for “ribonucleic acid”), deoxyribonucleic acid (short DNA for “deoxyribonucleic” acid “), plasmids, vaccines, extracellular vesicles, liposomes, secretomes, coagulation factors and albumin.
  • extracellular vesicles are preferably extracellular vesicles, preferably nano-vesicles (which are to be purified, i.e. concentrated) from the supernatant of a cell culture.
  • extracellular vesicles also includes exosomes, microvesicles and apoptotic bodies.
  • membranes with an exclusion limit (also molecular weight cut-off MWCO) of 100-1000 kDalton are preferably used.
  • the highest possible concentration of substances to be purified such as vesicles, ie the smallest possible amount of liquid, is aimed for. It is conceivable, for example, that a batch of 9 l is reduced according to the invention to a volume of approx. 100 ml, which leads to a corresponding increase in the density or concentration of the substances to be purified.
  • liquids other than cell supernatants can also be used, such as blood serum or plasma.
  • the present invention relates to the use of a centrifugal pump for carrying out a method according to one of Claims 12 to 14.
  • Figure 1 a schematic view of the centrifugal pump head with a vertical outlet as well as with an inclined outlet
  • FIG. 2 a schematic view of the centrifugal pump head with a vertical outlet and an inclined outlet, pipe section and a filter module directly connected to it
  • FIG. 3 a schematic view of the centrifugal pump head with a sloping outlet, pipe section, adapter and a filter module connected to the adapter.
  • Figure 1 a) shows a schematic sectional view of a centrifugal pump head with housing 10, rotating impeller 20 and outlet 30, the outlet pointing vertically upwards, i.e. the liquid leaves the pump head vertically upwards.
  • This has the disadvantage that air L can accumulate inside the pump head, which is undesirable.
  • the arrangement according to Figure 1 a) is not according to the invention.
  • the outlet 30 forms the highest point of the pump housing or the pump head, which - as shown in FIG. 1 b) - can be achieved, for example, by rotating the pump, for example, by 45 °. As can be seen from FIG. 1 b), the outlet 30 now forms the highest point, so that air can leave the pump head through the outlet 30 due to gravity.
  • FIG. 2 a shows an arrangement of the pump not according to the invention, a pipe section 40, on which the filter module 50 is arranged, being connected to the outlet.
  • the adapter 60 is angled or curved, so that the filter module 50 is vertical in spite of the inclined pipe section 40 or the outlet 10 arranged at the top.
  • the curved adapter is provided with flanges on both sides, by means of which it is fastened on the one hand to the pipe section 40 and on the other hand to the filter module 50.
  • the illustrated Luer-Lock outlet 70 of the adapter 60 can be used, for example, to arrange a pressure sensor or a sampling point without the need for a further flange connection.
  • the adapter 60 can be expanded from a small diameter or flange to a larger diameter or flange (preferably continuously).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • External Artificial Organs (AREA)

Abstract

La présente invention concerne un appareil de filtrage pour le filtrage de liquides, comprenant un module de filtre et une pompe, la pompe étant disposée de manière à transporter le liquide dans le module de filtre, le module de filtre étant disposé verticalement et la pompe étant une pompe centrifuge qui présente une sortie tangentielle qui forme le point le plus haut de la pompe centrifuge.
EP20811255.7A 2019-11-20 2020-11-18 Appareil de filtrage Pending EP4061514A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102019131362.7A DE102019131362A1 (de) 2019-11-20 2019-11-20 Filtrationsgerät
PCT/EP2020/082485 WO2021099357A1 (fr) 2019-11-20 2020-11-18 Appareil de filtrage

Publications (1)

Publication Number Publication Date
EP4061514A1 true EP4061514A1 (fr) 2022-09-28

Family

ID=73543230

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20811255.7A Pending EP4061514A1 (fr) 2019-11-20 2020-11-18 Appareil de filtrage

Country Status (4)

Country Link
EP (1) EP4061514A1 (fr)
CN (1) CN219072595U (fr)
DE (1) DE102019131362A1 (fr)
WO (1) WO2021099357A1 (fr)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4973408A (en) * 1987-04-13 1990-11-27 Keefer Bowie Reverse osmosis with free rotor booster pump
JP2981909B2 (ja) * 1990-06-20 1999-11-22 テルモ株式会社 液体流路を有する装置の気泡除去方法及びその装置
JP2825070B2 (ja) * 1995-05-23 1998-11-18 タイガー魔法瓶株式会社 貯液容器のポンプ設置構造
ITMI20031940A1 (it) * 2003-10-09 2005-04-10 Prodotti Chimici Alimentari Metodo per l'abbattimento degli agenti infettivi delle tse nella bile e derivati
EP1930034B1 (fr) 2006-12-07 2012-11-14 Thoratec LLC Pompe-oxygénateur de sang intégré, système extracorporel de maintien de la vie et procédé d'évacuation des bulles et d'amorçage d'un système extracorporel de maintien de la vie
EP2462965B1 (fr) * 2010-12-07 2015-05-20 ZOLL LifeBridge GmbH Procédé de remplissage et d'aération d'un dispositif destiné au traitement de sang extracorporel à l'aide du noyage cadencé de filtre
CN108883138A (zh) 2015-12-30 2018-11-23 加利福利亚大学董事会 增强细胞衍生的囊泡的生产和分离的方法

Also Published As

Publication number Publication date
WO2021099357A1 (fr) 2021-05-27
DE102019131362A1 (de) 2021-05-20
CN219072595U (zh) 2023-05-26

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