EP3995577A1 - Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression - Google Patents

Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression Download PDF

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Publication number
EP3995577A1
EP3995577A1 EP20206417.6A EP20206417A EP3995577A1 EP 3995577 A1 EP3995577 A1 EP 3995577A1 EP 20206417 A EP20206417 A EP 20206417A EP 3995577 A1 EP3995577 A1 EP 3995577A1
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EP
European Patent Office
Prior art keywords
molecule
controller
actuator
positively
output
Prior art date
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EP20206417.6A
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German (de)
English (en)
Inventor
Timothy Thomas Frei
Ching-Hsiang Chang
Maurice Filo
Mustafa Khammash
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Eidgenoessische Technische Hochschule Zurich ETHZ
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Eidgenoessische Technische Hochschule Zurich ETHZ
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Priority to EP20206417.6A priority Critical patent/EP3995577A1/fr
Priority to KR1020237019437A priority patent/KR20230106664A/ko
Priority to EP21802763.9A priority patent/EP4240845A1/fr
Priority to PCT/EP2021/081158 priority patent/WO2022096750A1/fr
Priority to AU2021374046A priority patent/AU2021374046A1/en
Priority to US18/252,194 priority patent/US20240018540A1/en
Priority to JP2023527285A priority patent/JP2023548385A/ja
Priority to CA3196471A priority patent/CA3196471A1/fr
Publication of EP3995577A1 publication Critical patent/EP3995577A1/fr
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • G16B5/10—Boolean models
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP20206417.6A 2020-11-09 2020-11-09 Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression Withdrawn EP3995577A1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP20206417.6A EP3995577A1 (fr) 2020-11-09 2020-11-09 Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression
KR1020237019437A KR20230106664A (ko) 2020-11-09 2021-11-09 세포에서 네트워크를 제어하기 위한 발현 시스템 및 방법 및 발현 시스템을 포함하는 세포
EP21802763.9A EP4240845A1 (fr) 2020-11-09 2021-11-09 Système d'expression et procédé de régulation d'un réseau dans une cellule et cellule comprenant le système d'expression
PCT/EP2021/081158 WO2022096750A1 (fr) 2020-11-09 2021-11-09 Système d'expression et procédé de régulation d'un réseau dans une cellule et cellule comprenant le système d'expression
AU2021374046A AU2021374046A1 (en) 2020-11-09 2021-11-09 Expression system and method for controlling a network in a cell and cell comprising the expression system
US18/252,194 US20240018540A1 (en) 2020-11-09 2021-11-09 Expression system and method for controlling a network in a cell and cell comprising the expression system
JP2023527285A JP2023548385A (ja) 2020-11-09 2021-11-09 細胞内のネットワークを制御するための発現システム及びその方法、並びに該発現システムを含む細胞
CA3196471A CA3196471A1 (fr) 2020-11-09 2021-11-09 Systeme d'expression et procede de regulation d'un reseau dans une cellule et cellule comprenant le systeme d'expression

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20206417.6A EP3995577A1 (fr) 2020-11-09 2020-11-09 Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression

Publications (1)

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EP3995577A1 true EP3995577A1 (fr) 2022-05-11

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EP20206417.6A Withdrawn EP3995577A1 (fr) 2020-11-09 2020-11-09 Système d'expression et procédé de commande d'un réseau dans une cellule et cellule comprenant le système d'expression

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066541A2 (fr) * 2009-11-30 2011-06-03 Trustees Of Boston University Convertisseurs chimiotactiques de circuits biologiques
WO2012012739A2 (fr) * 2010-07-22 2012-01-26 President And Fellows Of Harvard College Circuits classificateurs biologiques à entrées multiples pour cellules
WO2013155439A1 (fr) * 2012-04-13 2013-10-17 Massachusetts Institute Of Technology Calcul et circuits analogiques et à signaux mixtes dans des cellules vivantes
WO2016095934A2 (fr) * 2014-12-14 2016-06-23 El Abd Hisham Mohamed Magdy Nouveau dispositif génétique pour modifier génétiquement le comportement celulaire
EP3156493A1 (fr) * 2014-04-30 2017-04-19 Tsinghua University Utilisation de répresseur de transcription tale pour la construction modulaire d'une lignée de gènes synthétiques dans une cellule de mammifère
WO2019027414A1 (fr) * 2017-07-31 2019-02-07 Massachusetts Institute Of Technology Détecteurs de micro-arn à entrées multiples haute performance et leurs utilisations

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066541A2 (fr) * 2009-11-30 2011-06-03 Trustees Of Boston University Convertisseurs chimiotactiques de circuits biologiques
WO2012012739A2 (fr) * 2010-07-22 2012-01-26 President And Fellows Of Harvard College Circuits classificateurs biologiques à entrées multiples pour cellules
WO2013155439A1 (fr) * 2012-04-13 2013-10-17 Massachusetts Institute Of Technology Calcul et circuits analogiques et à signaux mixtes dans des cellules vivantes
EP3156493A1 (fr) * 2014-04-30 2017-04-19 Tsinghua University Utilisation de répresseur de transcription tale pour la construction modulaire d'une lignée de gènes synthétiques dans une cellule de mammifère
WO2016095934A2 (fr) * 2014-12-14 2016-06-23 El Abd Hisham Mohamed Magdy Nouveau dispositif génétique pour modifier génétiquement le comportement celulaire
WO2019027414A1 (fr) * 2017-07-31 2019-02-07 Massachusetts Institute Of Technology Détecteurs de micro-arn à entrées multiples haute performance et leurs utilisations

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
AOKI STEPHANIE K ET AL: "A universal biomolecular integral feedback controller for robust perfect adaptation", NATURE, MACMILLAN JOURNALS LTD., ETC, LONDON, vol. 570, no. 7762, 19 June 2019 (2019-06-19), pages 533 - 537, XP036817374, ISSN: 0028-0836, [retrieved on 20190619], DOI: 10.1038/S41586-019-1321-1 *
BAOJUN WANG ET AL: "Customizing cell signaling using engineered genetic logic circuits", TRENDS IN MICROBIOLOGY, vol. 20, no. 8, 6 June 2012 (2012-06-06), pages 376 - 384, XP028430531, ISSN: 0966-842X, [retrieved on 20120508], DOI: 10.1016/J.TIM.2012.05.001 *
CAROLA ENGLERROMY KANDZIASYLVESTRE MARILLONNET: "A one pot, one step, precision cloning method with high throughput capability", PLOS ONE, vol. 3, no. 11, 2008, XP002613221, DOI: 10.1371/journal.pone.0003647
GABRIELE LILLACCI ET AL: "Synthetic control systems for high performance gene expression in mammalian cells", NUCLEIC ACIDS RESEARCH, vol. 46, no. 18, 7 September 2018 (2018-09-07), pages 9855 - 9863, XP055753584, ISSN: 0305-1048, DOI: 10.1093/nar/gky795 *
MICHAEL E LEEWILLIAM C DELOACHEBERNARDO CERVANTESJOHN E DUEBER: "A highly characterized yest toolkit for modular, multipart assembly", ACS SYNTHETIC BIOLOGY, vol. 4, no. 9, 2015, pages 975 - 986
SIMON AUSLÄNDER ET AL: "From gene switches to mammalian designer cells: present and future prospects", TRENDS IN BIOTECHNOLOGY, vol. 31, no. 3, 13 December 2012 (2012-12-13), pages 155 - 168, XP055117371, ISSN: 0167-7799, DOI: 10.1016/j.tibtech.2012.11.006 *
WIELAND MARKUS ET AL: "Engineering Molecular Circuits Using Synthetic Biology in Mammalian Cells", ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, vol. 3, no. 1, 29 March 2012 (2012-03-29), US, pages 209 - 234, XP055792389, ISSN: 1947-5438, Retrieved from the Internet <URL:https://www.annualreviews.org/doi/pdf/10.1146/annurev-chembioeng-061010-114145> DOI: 10.1146/annurev-chembioeng-061010-114145 *

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