EP3893904A4 - Génération et cryoconservation de cellules endothéliales cornéennes de grade clinique dérivées de cellules souches pluripotentes - Google Patents

Génération et cryoconservation de cellules endothéliales cornéennes de grade clinique dérivées de cellules souches pluripotentes Download PDF

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Publication number
EP3893904A4
EP3893904A4 EP19895410.9A EP19895410A EP3893904A4 EP 3893904 A4 EP3893904 A4 EP 3893904A4 EP 19895410 A EP19895410 A EP 19895410A EP 3893904 A4 EP3893904 A4 EP 3893904A4
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EP
European Patent Office
Prior art keywords
cryopreservation
generation
stem cell
endothelial cells
pluripotent stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19895410.9A
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German (de)
English (en)
Other versions
EP3893904A1 (fr
Inventor
S. Amer RIAZUDDIN
John GOTTSCH
Shahid Khan
Muhammad Ali
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Publication of EP3893904A1 publication Critical patent/EP3893904A1/fr
Publication of EP3893904A4 publication Critical patent/EP3893904A4/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19895410.9A 2018-12-12 2019-12-12 Génération et cryoconservation de cellules endothéliales cornéennes de grade clinique dérivées de cellules souches pluripotentes Pending EP3893904A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862778862P 2018-12-12 2018-12-12
PCT/US2019/065907 WO2020123769A1 (fr) 2018-12-12 2019-12-12 Génération et cryoconservation de cellules endothéliales cornéennes de grade clinique dérivées de cellules souches pluripotentes

Publications (2)

Publication Number Publication Date
EP3893904A1 EP3893904A1 (fr) 2021-10-20
EP3893904A4 true EP3893904A4 (fr) 2022-08-17

Family

ID=71077494

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19895410.9A Pending EP3893904A4 (fr) 2018-12-12 2019-12-12 Génération et cryoconservation de cellules endothéliales cornéennes de grade clinique dérivées de cellules souches pluripotentes

Country Status (6)

Country Link
US (1) US20220025325A1 (fr)
EP (1) EP3893904A4 (fr)
JP (1) JP2022513736A (fr)
AU (1) AU2019397500A1 (fr)
CA (1) CA3122102A1 (fr)
WO (1) WO2020123769A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024071382A1 (fr) * 2022-09-30 2024-04-04 株式会社セルージョン Cellule souche pluripotente et cellule différenciée et induite à partir d'une cellule souche ayant une fonction de sécrétion de glp-1

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2765188A1 (fr) * 2011-10-06 2014-08-13 Keio University Procédé pour la production de cellules endothéliales de la cornée
WO2017141926A1 (fr) * 2016-02-15 2017-08-24 Kyoto Prefectural Public University Corporation Cellule endothéliale cornéenne fonctionnelle humaine et application associée
WO2017190136A1 (fr) * 2016-04-29 2017-11-02 The Regents Of The University Of California Induction de cellules endothéliales cornéennes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2765188A1 (fr) * 2011-10-06 2014-08-13 Keio University Procédé pour la production de cellules endothéliales de la cornée
WO2017141926A1 (fr) * 2016-02-15 2017-08-24 Kyoto Prefectural Public University Corporation Cellule endothéliale cornéenne fonctionnelle humaine et application associée
WO2017190136A1 (fr) * 2016-04-29 2017-11-02 The Regents Of The University Of California Induction de cellules endothéliales cornéennes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALI MUHAMMAD ET AL: "Comparative transcriptome analysis of hESC- and iPSC-derived corneal endothelial cells", EXPERIMENTAL EYE RESEARCH, vol. 176, 6 September 2018 (2018-09-06), LONDON., pages 252 - 257, XP055939722, ISSN: 0014-4835, Retrieved from the Internet <URL:https://www.sciencedirect.com/science/article/pii/S0014483518303026/pdfft?md5=7504765bee36821c4047aac40f3d0103&pid=1-s2.0-S0014483518303026-main.pdf> DOI: 10.1016/j.exer.2018.08.023 *
JIAGANG J. ZHAO ET AL: "Generation of Human Corneal Endothelial Cells via In Vitro Ocular Lineage Restriction of Pluripotent Stem Cells", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, vol. 57, no. 15, 21 December 2016 (2016-12-21), US, pages 6878 - 6884, XP055435736, ISSN: 1552-5783, DOI: 10.1167/iovs.16-20024 *
KATHRYN L. MCCABE ET AL: "Efficient Generation of Human Embryonic Stem Cell-Derived Corneal Endothelial Cells by Directed Differentiation", PLOS ONE, vol. 10, no. 12, 21 December 2015 (2015-12-21), pages e0145266, XP055556295, DOI: 10.1371/journal.pone.0145266 *
MOHIT PAREKH ET AL: "Concise Review: An Update on the Culture of Human Corneal Endothelial Cells for Transplantation : Corneal Endothelial Cell Culture", STEM CELLS TRANSLATIONAL MEDICINE, vol. 5, no. 2, 23 December 2015 (2015-12-23), US, pages 258 - 264, XP055489663, ISSN: 2157-6564, DOI: 10.5966/sctm.2015-0181 *
MUHAMMAD ALI ET AL: "Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, vol. 59, no. 6, 15 May 2018 (2018-05-15), US, pages 2437, XP055722295, ISSN: 1552-5783, DOI: 10.1167/iovs.17-22927 *
See also references of WO2020123769A1 *

Also Published As

Publication number Publication date
CA3122102A1 (fr) 2020-06-18
US20220025325A1 (en) 2022-01-27
AU2019397500A1 (en) 2021-06-17
EP3893904A1 (fr) 2021-10-20
JP2022513736A (ja) 2022-02-09
WO2020123769A1 (fr) 2020-06-18

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