EP3851518A4 - Method for producing intestinal cells from pluripotent stem cells - Google Patents

Method for producing intestinal cells from pluripotent stem cells Download PDF

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Publication number
EP3851518A4
EP3851518A4 EP19859574.6A EP19859574A EP3851518A4 EP 3851518 A4 EP3851518 A4 EP 3851518A4 EP 19859574 A EP19859574 A EP 19859574A EP 3851518 A4 EP3851518 A4 EP 3851518A4
Authority
EP
European Patent Office
Prior art keywords
cells
pluripotent stem
stem cells
producing intestinal
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19859574.6A
Other languages
German (de)
French (fr)
Other versions
EP3851518A1 (en
Inventor
Shoen Kume
Nobuaki Shiraki
Kazuya Maeda
Hiroyuki Kusuhara
Masaya Ishikawa
Teruhiko WATANABE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanto Chemical Co Inc
Tokyo Institute of Technology NUC
University of Tokyo NUC
Original Assignee
Kanto Chemical Co Inc
Tokyo Institute of Technology NUC
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2019117748A external-priority patent/JP7377486B2/en
Application filed by Kanto Chemical Co Inc, Tokyo Institute of Technology NUC, University of Tokyo NUC filed Critical Kanto Chemical Co Inc
Publication of EP3851518A1 publication Critical patent/EP3851518A1/en
Publication of EP3851518A4 publication Critical patent/EP3851518A4/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/25—Urinary tract cells, renal cells
    • C12N2502/256—Renal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/70—Non-animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
EP19859574.6A 2018-09-10 2019-09-09 Method for producing intestinal cells from pluripotent stem cells Pending EP3851518A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018168957 2018-09-10
JP2019117748A JP7377486B2 (en) 2018-09-10 2019-06-25 Method for producing intestinal cells from pluripotent stem cells
PCT/JP2019/035358 WO2020054659A1 (en) 2018-09-10 2019-09-09 Method for producing intestinal cells from pluripotent stem cells

Publications (2)

Publication Number Publication Date
EP3851518A1 EP3851518A1 (en) 2021-07-21
EP3851518A4 true EP3851518A4 (en) 2022-06-22

Family

ID=69778328

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19859574.6A Pending EP3851518A4 (en) 2018-09-10 2019-09-09 Method for producing intestinal cells from pluripotent stem cells

Country Status (2)

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EP (1) EP3851518A4 (en)
WO (1) WO2020054659A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317415B (en) * 2022-03-07 2022-06-03 天九再生医学(天津)科技有限公司 Method for directional differentiation of human pluripotent stem cells into multiple lineage large intestine organoids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140199700A1 (en) * 2010-11-02 2014-07-17 Lsip, Llc Method for producing intestinal cells
WO2016123474A1 (en) * 2015-01-30 2016-08-04 The University Of North Carolina At Chapel Hill Methods to generate gastrointestinal epithelial tissue constructs
WO2016147975A1 (en) * 2015-03-13 2016-09-22 国立研究開発法人医薬基盤・健康・栄養研究所 Intestinal epithelioid cells
WO2016193441A2 (en) * 2015-06-03 2016-12-08 Takara Bio Europe Ab Maturation of mammalian hepatocytes
WO2019021990A1 (en) * 2017-07-28 2019-01-31 国立大学法人大阪大学 Enterocyte-like cells

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2473182A1 (en) * 2002-02-08 2003-08-14 Acologix, Inc. Compositions and methods for treatment of vitamin d deficiency
MX2008009764A (en) * 2006-01-31 2009-03-05 Health Research Inc Method for identifying altered vitamin d metabolism.
EP2610335B1 (en) 2010-08-25 2017-03-01 National Institute Of Agrobiological Sciences Dried hydrogel, dried vitrigel film, and processes for producing these
JP5933223B2 (en) 2010-11-12 2016-06-08 国立研究開発法人農業生物資源研究所 Cell culture chamber and manufacturing method thereof, and tissue model using the cell culture chamber and manufacturing method thereof
CN105451818A (en) * 2013-06-05 2016-03-30 萨克生物研究学院 Vitamin D receptor agonists to treat diseases involving CXCL12 activity
JP6351018B2 (en) 2014-02-21 2018-07-04 国立大学法人 熊本大学 Stem cell differentiation induction method using amino acid composition modified medium, and medium kit containing the medium
JP6822885B2 (en) 2017-03-30 2021-01-27 バンドー化学株式会社 Conveyor belt
JP2019117748A (en) 2017-12-27 2019-07-18 スタンレー電気株式会社 Luminaire

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140199700A1 (en) * 2010-11-02 2014-07-17 Lsip, Llc Method for producing intestinal cells
WO2016123474A1 (en) * 2015-01-30 2016-08-04 The University Of North Carolina At Chapel Hill Methods to generate gastrointestinal epithelial tissue constructs
WO2016147975A1 (en) * 2015-03-13 2016-09-22 国立研究開発法人医薬基盤・健康・栄養研究所 Intestinal epithelioid cells
WO2016193441A2 (en) * 2015-06-03 2016-12-08 Takara Bio Europe Ab Maturation of mammalian hepatocytes
WO2019021990A1 (en) * 2017-07-28 2019-01-31 国立大学法人大阪大学 Enterocyte-like cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
JABAJI ZIYAD ET AL: "Use of Collagen Gel as an Alternative Extracellular Matrix for the In Vitro and In Vivo Growth of Murine Small Intestinal Epithelium", TISSUE ENGINEERING. PART C, METHODS DEC 2008, vol. 19, no. 12, 1 December 2013 (2013-12-01), US, pages 961 - 969, XP055919226, ISSN: 1937-3384, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833386/pdf/ten.tec.2012.0710.pdf> [retrieved on 20220509], DOI: 10.1089/ten.tec.2012.0710 *
OZAWA TATSUYA ET AL: "Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine", SCIENTIFIC REPORTS, vol. 5, no. 1, 12 November 2015 (2015-11-12), XP055781004, Retrieved from the Internet <URL:http://www.nature.com/articles/srep16479.pdf> [retrieved on 20210302], DOI: 10.1038/srep16479 *
See also references of WO2020054659A1 *
SOICHIRO OGAKI ET AL: "A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells", SCIENTIFIC REPORTS, vol. 5, no. 1, 30 November 2015 (2015-11-30), XP055641733, DOI: 10.1038/srep17297 *
SOICHIRO OGAKI ET AL: "Wnt and Notch Signals Guide Embryonic Stem Cell Differentiation into the Intestinal Lineages", STEM CELLS, vol. 31, no. 6, 22 June 2013 (2013-06-22), pages 1086 - 1096, XP055177743, ISSN: 1066-5099, DOI: 10.1002/stem.1344 *
TAKEZAWA ET AL: "Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 131, no. 1, 13 July 2007 (2007-07-13), pages 76 - 83, XP022151842, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2007.05.033 *

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Publication number Publication date
WO2020054659A1 (en) 2020-03-19
EP3851518A1 (en) 2021-07-21

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