EP3806822A1 - Composition and kit for use in a method of embryo transfer - Google Patents
Composition and kit for use in a method of embryo transferInfo
- Publication number
- EP3806822A1 EP3806822A1 EP19749780.3A EP19749780A EP3806822A1 EP 3806822 A1 EP3806822 A1 EP 3806822A1 EP 19749780 A EP19749780 A EP 19749780A EP 3806822 A1 EP3806822 A1 EP 3806822A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- implantation
- promoter
- embryo
- uterus
- uterine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/57—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0024—Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue
Definitions
- This invention relates to a an agent and an apparatus for use in a minimally-invasive medical procedure of embryo transfer utilizing a uterine transfer catheter that deposits fertilized eggs into the uterus whereby implantation of an embryo occurs.
- IVF Human in vitro fertilization
- E embryo transfer
- the IVF/ET procedure typically involves hormonal stimulation of the female to initially suppress her natural ovulation, and then stimulate development of ovarian follicles with fertility drugs.
- the mature eggs are harvested from the ovary using a needle and then sorted by maturity and egg quality.
- Preferred eggs are then mixed with sperm from the male to be fertilized.
- the embryo(s) mature and are transferred, along with a volume of fluid, to the uterus using a delivery catheter.
- the delivery catheter is made of soft plastic material to avoid damage to the endometrium of the uterus.
- the delivery catheter is guided through the cervix to the uterus by a physician where the loaded embryos within the fluid is deposited, completing the ET.
- One method of catheter loading is referred to as "the transfer bubble method" and has been universally accepted by professionals and has been in place over four decades.
- the catheter is loaded with a series of alternate air and transfer liquid (e.g., saline, P-1 or glycerine) in volumes approximating 3 microliters. Volumes of air separate the transfer liquid to keep the embryos contained within the fluid and also to ease visualization of the components.
- a series of alternate air and transfer liquid e.g., saline, P-1 or glycerine
- volumes of air separate the transfer liquid to keep the embryos contained within the fluid and also to ease visualization of the components.
- about 100 microliters of air is also ejected with the quantity of medium and transfer liquid containing the embryos.
- the transferred air within the uterus can sometimes act as a barrier that prohibits movement of the embryos from the site of ejection to the optimal location for implantation within the uterus.
- This air bubble creates an area of surface tension to which the embryos may adhere and remain positioned, prohibiting interaction of the embryo with the receptive surface of the uterus.
- latent embryos can attach themselves to the internal walls of the catheter requiring further manipulation of the embryos and thereby decreasing the likelihood of successful ET.
- the high cost of the IVF procedure combined with this low probability of success compels couples to deliver multiple embryos to increase the chance of at least one successful implantation.
- unwanted multiple births result and jeopardize the health of mother and/or children, which has recently raised questions concerning the medical ethics of such practice. If a higher pregnancy rate could be achieved, the need for multiple embryo delivery would be lessened or possibly eliminated.
- Hormonal delivery via the blood stream after systemical or intramuscular injection has several disadvantages; it causes delayed delivery of the hormone at the target site and a significant amount of of hormone does not reach the site where it is needed. As it is a burden for any female to receive hormones the amount of hormone should be as low as possible.
- An agent and an apparatus for use in artificial embryonic implantation are provided for improving fertility rates by eliminating risks for a transferred embryo and for reducing risks for insufficient nidation.
- the present invention provides means to be able to prepare a uterus before an embryo transfer procedure such that the conditions at the implantation site are optimal within a small time window before and/or during transfer.
- time window for nidation or implantation of an embryo is a few days so that the time of introduction was deemed not to be too critical.
- best chances for successful implantation and pregnancy are found when nidation or implantation occurs within a narrow time window. It has been surprisingly found that the time window is not a few days as assumed in the past, but only a few hours, such as up to 36 hours, for example up to 24 hours.
- the present invention provides a formulation and a device for use in a method of embryo transfer ("ET") that improves fertility rates by hormonally enhancing the uterine wall of a female at the time of ET, by stimulating pregnancy supporting uterine factors, and by protecting the embryo by eliminating transferred air during the procedure.
- ET embryo transfer
- the terms“nidation” and“implantation” are used exchangeably. Both terms refer to the stage at which an embryo adheres to the wall of an uterus and prenatal development starts.
- implantation promoter refers to a formulation that can comprise an agent or a composition comprising at least one agent, wherein the agent promotes implantation.
- An agent promotes implantation by having a positive influence on conceiving and/or supporting adherence and growth of a fertilsed embryo or conceptus for a successful pregnancy, for example by stimulating the luteal phase and/or by activating or stimulating uterine factors.
- nanoformulation or“nanosize formulation” refers to a formation that comprises at least part of the active agent in nanosize, i.e. a size in the range of nm, such as 1 to 1000 nm, for example 1 to 500 nm.
- uterine factors when used in this application refers to those factors in uterine that react to, are involved in, or are influenced, activated, promoted by implanation and/or pregnancy.
- uterine factors are aquaporins, epithelial sodium channels, prostaglandins, activators of the sodium pump, or HNE-1 protein.
- artificial embryonic implantation or “embryo transfer procedure” refer to methods where a fertilized egg or embryo is introduced into the uterus of a female for implantation or nidation.
- window of implantation refers to a time range or time period wherein the uterus is receptive for implantation, this time range can be the time between preparation of a uterus and delivery of a fertilzed egg or embryo. It has been found that this window should be used for an optimized and successful implantation.
- the formulation used as implantation promoter can comprise one or more implantation promoting agents, it can also comprise further components like additives, carriers, excipients, etc.
- the formulation can be in any convenient form, it can be in fluid form such as a liquid.
- the formulation should be fluid to be administered by a device like a catheter.
- examples are formulations wherein the implantation promoter is dispersed in a pharmaceutically acceptable carrier or excipient, such as dissolved, dispersed, pulverised, microencapsulated. It might be useful to apply at least part of the implantation promoter in a form that stays at the site where it is administered and does not flow away after administration. Therefore, it can be useful to use a formulation of an implementation promoter in the form of a gel, emulsion, or any other form that is known to stay at the site. Direct administration of the implantation promoter, such as hormones into the uterus may be achieved in different ways.
- the implantation promoter can be administered either separated from or together with delivery of the embryo.
- One example is including the hormones in the milieu of solutions utilized during the transfer of one or more embryos into the endometrium during ET. Luteal support is maintained by slow dissolution of the deposited hormones by direct injection into the uterus, as opposed to introducing the hormones systemically. Further direct administration allows for optimizing the delivery time of these hormones in the preparation of the uterus prior to or simultaneously with the delivery of a fertilized egg at the site of implantation.
- the amount of implantation promoter can be lower compared to known methods where hormones are administered systemically or intramuscularly.
- the implantation promoter can be administered before and/or during and/or after the embryo transfer procedure. It has been found that chances for successful implantation and pregnancy are increased when preparation of the uterine site and transfer of embryo are in a close timely relationship and/or are done in parallel.
- the implantation promoter for use in a method for artificial embryonic implantation comprises the use in a method of targeted intrauterine administration in a timed sequence of events to precisely establish a progesterone dominant uterus that is primed and ready to receive a developing blastocyst for implantation
- Quantitative administration of the hormonal solutions can for example be accomplished by a modified delivery catheter that comprises an outer sheath having a proximal end and a distal end.
- An inner lumen is arranged to be slidably disposed within the outer sheath.
- the inner lumen includes a proximal end, a distal end, and a passageway therethrough.
- the inner lumen includes at least one visual marker situated on the exterior surface adjacent its distal end thereof to enable accurate volumes of fluid media in which one or more embryos are situated to be drawn into the deliver catheter.
- one aspect of the present invention relates to an implantation promoter for use in a method for artificial embryonic implantation in a female subject wherein the method comprises direct administration of the implantation promoter at a target site of a uterus and during a time window of implantation. It has been found that improved results regarding successful implantation and pregnancy are obtained when the time window for administration of implantation promoter is within a range of up to 36 hours, such as up to 24 hours, for example 0.5 to 12 hours before embryonic transfer. It is also possible to administer the implantation promoter at the time of and/or during and/r after transfer procedure.
- the amount of implantation promoter should be sufficient to promote stimulation and/or activation of uterine factors at a target site.
- one aspect of the present invention relates to a delivery catheter comprising an outer sheath (10) having a proximal end and a distal end; and an inner lumen (36) slidably disposed within said outer sheath (10), said inner lumen (36) having a proximal end, a distal end, and an internal passageway (34) therein for receiving at least one embryo and an implantation promoter and optionally other media, said inner lumen (36) including at least one visual marker (38) situated on the exterior surface thereof adjacent its distal end indicating a predetermined volume to enable aspiration of an accurate volume of said implantation promoter and optionally of said one or more media into said inner lumen (36), wherein said at least one visual marker (38) comprises a plurality of visual markers (38), each visual marker (38) indicating a volume of media to be aspirated into said inner lumen (36), for use in a method for introduction of an embryo to a target site of a uterus of a female in an embryo transfer procedure.
- One advantage of the catheter as described above is the possibility to apply a formulation very exactly and precisely because markers are provided which allow to dose any fluid correctly.
- FIG. 1 is an elevational view of an embodiment of the delivery catheter for use in the present invention with a syringe shown attached at the proximal end thereof, the syringe including a barrel and a plunger disposed therein.
- FIG. 2 is a cross-sectional view taken along line 2-2 of FIG. 1.
- FIG. 3 is a cross-sectional view taken along line 3-3 of FIG. 1.
- FIG. 4 is a cross-sectional view of a portion of an embodiment of the delivery catheter for use in the present invention wherein the inner lumen is illustrated as retaining therein a first portion comprised of a wash fluid, a second portion comprised of an oil, and a third portion comprised of one or more embryos disposed within a fluid culture medium.
- FIG. 5 is a cross-section view of a portion of an embodiment of the delivery catheter for use in he present invention wherein the inner lumen is illustrated as retaining therein a first portion comprised of a wash fluid, a second portion comprised of an oil, and a third portion comprised of one or more embryos disposed within a fluid culture medium.
- FIG. 6 is a front end view of the delivery catheter for use in the present invention.
- FIG. 7 is an illustration of several embryos implanted on the wall of a uterus after an embryo transfer procedure has been conducted.
- the present invention is concerned with an implantation promoter for use in a method for artificial embryonic implantation in a female subject wherein the method comprises a. administering a formulation comprising at least one implantation promoter at a target site; and b. depositing a fertilized embryo at the prepared site within a window of implantation.
- the implantation promoter for use of the present invention is a formulation comprising at least one agent or a composition which can prepare the uterus for implantation, for example by promoting the activation or stimulation of uterine factors at a target site.
- Implantation promoting agents and compositions are known in the art and those that are known can be used for the present use.
- Examples for an implantation promoter that can be used according to the present invention are progesterone, estrogen, or a combination thereof. Good results are obtained when using progesterone for preparing the uterine for nidation or implantation.
- An formulation of an implantation promoter can comprise only one hormone, such as progesterone or estrogen, or a combination, and it can comprise further compounds, agents or factors that are involved in fertilization and nidation and support of embryo growth.
- Useful additional agents are those that contribute to stimulation and/or activation of uterine factors.
- the amount to be used has to be sufficient to promote implantation at the target site, for example by activation of uterine factors.
- Useful amounts of implantation promoters are known and the skilled person knows about useful amounts and how to determine an optimal amount in a specific case by using routine experiments. The amount depends on the type and form of promoter used, the patient to be treated, the number of applications and other conditions.
- a useful concentration of the implantation promoters is in the range between approximately 0.001 micrograms to 1000 milligrams amount, such as about 1 pg to about 500 mg, for example between about 100 pg and 250 mg.
- Administration of the implantation promoter can be done either once or in two or more dosages. In one embodiment, an implantation promoter is administered within 12 hours before the transfer procedure and a second time during embryo transfer.
- the implantation promoter for use in the method of the present invention can be any progesterone or estrogen preparation that is used in the field of artificial fertilization and embryo transfer procedure. As it has been found critical that the nidation site is prepared for reception of the embryo, it can be useful to use a formulation of the implantation promoter with delayed delivery for preparation. Delayed delivery preparations are well-known. On the other hand, when the implantation promoter is used at the time of embryo transfer, it can be useful to have a fast releasing preparation of the implantation promoter, for example in the form of a nano-emulsion.
- a nanosize formulation comprising a implantation promoter can further improve the chances for a successful nidation and pregnancy. Not only does the nanoformulation improve the pharmokinetic properties of the implantation promoter but it also allows to fine-tune interaction with other active agents, for example between progesterone, estrogen and uterine factors, and helps in establishing a more successful window. Furthermore, the delivery of the implantation promoter as nanoformulation addresses a long-standing problem of reducing volume of fluid to be administered.
- the time window for implantation of an embryo is critical. It has been surprisingly found that the time period wherein the uterus or the target site at the uterine wall, resepctievly, is receptive for the embryo and allows nidation of the embryo and, thus, makes a successful nidation and pregnancy more probable is shorter than expected in the past. It has been found that best results are obtained when the time window is up to about 36 hours.
- the time span between the time where the target site is prepared, for example by administration of an implantation promoter, and the time of transfer of embryo are within a range of at most 36 hours, such as 0 to 24 hours, in particular 0 to 12 hours.
- the implantation promoter for use of the present invention can be used for female subjects. It has been found, that the implantation promoter for use of the present invention is not only useful for humans, but can be used also for mammals, where embryo transfer or artificial fertilization respectively, are used for breeding. Examples are cattle, such as cows, horses, rare animals that are breeded in zoo or for scientific purposes and so on.
- the optimal amount and time window for delivery of implantation promoter can be identified for any species as is known in the art.
- an embodiment of the delivery catheter is illustrated as including an outer sheath 10 being in the form of a hollow tube and provided towards its proximal end with a hub 20 provided with a series of axially extending fins 24 to assist in manipulation.
- the distal end of the outer sheath 10 is provided at intervals of a predetermined distance, e.g., one centimeter, with gradations 28 of any suitable measure, e.g., microliters.
- the gradations are marked as a plurality of rings about the distal end, and which may be slightly indented into the outer surface thereof.
- the gradations 28 may be of a distinctive color.
- the remote distal tip 32 of the outer sheath 10 is chamfered for minimal trauma.
- the outer sheath 10 is formed of any suitable material, and preferably a generally rigid plastic material such as TEFLON®
- the outer sheath 10 is adapted to accommodate therewithin an inner lumen 36 having an external diameter such as to be an easy sliding fit within the outer sheath 10 and an internal passageway 34 having a diameter of a size to readily accommodate an embryo.
- the inner lumen 36 is provided at its proximal end with a hub 40, the hub 40 being provided about its external surface with a plurality of axially extending fins 44 to assist manipulation.
- the inner lumen 36 is provided with an inner bore 42 (FIG. 2) that is in open communication with the internal passageway 34.
- the hubs 20 and 40 are adapted such that they interlock in their closed position as shown in FIGS. 1 and 5.
- the hubs 20 and 40 are provided to enable a physician and/or an embryologist to hold the outer sheath 10 with one hand while using the other hand to slide the inner lumen 36 through the outer sheath 10 during an embryo transfer procedure.
- the distal end of the inner lumen 36 is provided at intervals of a predetermined distance, e.g., one centimeter, with gradations 38 of any suitable measure, e.g., microliters.
- the gradations 38 are marked as a plurality of rings about the distal end, and may be slightly indented into the outer surface thereof. Alternatively, the gradations 38 may be of a distinctive color to allow the operator to precisely aspirate an exact quantity of a fluid medium to achieve a consistent protocol.
- the remote distal tip 46 of the inner lumen 36 is provided with a smoothly radiused chamfer for minimal trauma to the uterine tissues.
- the inner lumen 36 in accordance with this embodiment of the invention is preferably soft and flexible and formed of a biologically acceptable synthetic polymer.
- FIGS. 1-4 there is shown a standard syringe 52 having a barrel 56 and a plunger 60 disposed therein.
- the syringe 52 is shown with the plunger 60 disposed fully within the length of the barrel 56.
- the syringe 52 includes a distal end 61 which is sized to snugly fit within the inner bore 42 of the inner lumen 36.
- the distal end 61 of the syringe 52 is inserted into the inner bore 42 of the inner lumen 36 with the plunger 60 disposed fully within the length of the barrel 56 of the syringe 52.
- a predetermined amount of a first fluid medium e.g., from 6 to 9 microliters of a wash fluid 64, is drawn into the internal passageway 34 of the inner lumen 36 and into the barrel 56 of the syringe 52 by withdrawing the plunger 60 along a portion of the length of the barrel 56.
- an excess amount of the wash fluid 64 is ejected from the syringe 52 and internal passageway 34 by depressing the plunger 60.
- an accurate volume of wash fluid 64 can be obtained within the internal passageway 34.
- the ET delivery catheter 6 is then held vertically to enable air to escape out the remote distal tip 46 of the inner lumen 36. Often, the sides of the ET delivery catheter 6 are tapped by the forefingers to loosen any air bubbles from the internal sides of the barrel 56. Referring now to FIG.
- a predetermined amount of a second fluid medium e.g., approximately 3 microliters of a separation oil 68
- a separation oil 68 may be utilized for the purpose of separating the wash fluid 64 from the culture medium containing one or more embryos 72.
- One particular product which could be utilized as a separation oil in accordance with the present invention is an enhanced oil for tissue culture manufactured by Conception Technologies of San Diego, Calif under catalog numbers OTC-100 (100 ml.) and OTC-500 (500 ml_).
- an accurate volume of the separation oil 68 within the internal passageway 34 can be achieved.
- unwanted air and any unneeded volume of separation oil 68 can be expelled through the remote distal tip 46 by depressing the plunger 60 of the syringe 52 and/or tapping with forefingers to loosen air bubbles.
- the gradations 38 located at the distal end of the inner lumen 36 enable an operator to draw a predetermined amount of a third fluid medium, e.g., approximately 3-6 microliters of a fluid culture medium containing one or more embryos 72, into the internal passageway 34 of the inner lumen 36.
- a third fluid medium e.g., approximately 3-6 microliters of a fluid culture medium containing one or more embryos 72
- the third fluid medium in which the embryos 72 are located is shown as being situated adjacent the separation oil 68.
- the fluid culture medium in which the embryos 72 are located includes a water-based portion and sometimes, an oil-based portion. When an oil-based portion is used, it is done so to prevent evaporation of the water-based portion and prevent dehydration of the embryos 72.
- One product which is suitable for utilizing as the oil-based portion of the fluid culture medium is the enhanced oil for tissue culture manufactured by Conception Technologies of San Diego, Calif under catalog numbers OTC-100 (100 mL) and OTC-500 (500 mL), as mentioned in connection with the separation oil described above.
- the three fluid media e.g., the wash fluid 64, the separation oil 68, and the plurality of embryos 72 disposed within a fluid culture medium are shown disposed within the internal passageway 34 of the inner lumen 36.
- the separation oil 68 is utilized as a replacement to the volume of air which under existing techniques is utilized to separate the wash fluid 64 from the embryos 72.
- the drawbacks associated with the use of air in embryo transfer procedures is eliminated.
- the use of a separation oil 68 will facilitate movement of the embryos 72 from the site of ejection to the optimal location for implantation within the uterus and will facilitate interaction between the embryo 72 and the receptive surface of the uterus.
- Embryos 72 will flow more readily within a liquid environment than a gaseous (air) environment.
- ingredients may be added to the separation oil 68 and/or wash fluid 64, including progesterone and/or estrogen to stimulate the luteal phase which determines the time of ovulation within the menstrual cycle and establishes the peak moment of receptivity within the uterus.
- progesterone and/or estrogen to stimulate the luteal phase which determines the time of ovulation within the menstrual cycle and establishes the peak moment of receptivity within the uterus.
- hormones needed for stimulation of the luteal phase may be added to the separation oil 68 and/or the wash fluid 64 and delivered directly into the uterus during an ET procedure utilizing the delivery catheter 6 described herein.
- the hormones may be introduced directly into the uterus utilizing any other suitable delivery device and may be delivered into the uterus at any point in time, either prior to or during the ET procedure.
- One embodiment of the present invention is a method to hormonally prepare the uterus prior to the introduction of a fertilized egg during embryonic implantation.
- Embryonic Implantation in humans is a complex process, part of which requires hormonal preparation of the uterus prior to the involvement of a fertilized egg.
- This preparation has been described in general terms as“the window of implantation” of the ovulation cycle whereby progesterone is secreted by the corpus luteum, situated on the developing ovary, and travels down the fallopian tube into the uterus in a slow but timely manner each month of a woman’s hormonal cycle. Consequently, embryonic implantation is a synchronized process between hormonal preparation and implantation of a fertilized egg.
- IM intramuscular injection
- PV peri-vaginal delivery
- products that contain micronized progesterone such as: gels, effervescent tablets, creams, suppositories, etc.
- IM intramuscular injection
- PV peri-vaginal delivery
- These forms of hormonal delivery enter the bloodstream and the progesterone molecules eventually reach the outside of the uterus (myometrium) and propagate toward the endometrial innermost layer of the uterus, where implantation occurs.
- One embodiment of the present invention provides a means to control the bioavailability of progesterone within an acceptable concentration and time range in order to establish a progesterone dominant uterus (over antagonist-estrogen) and achieve implantation (and ultimately pregnancy) more often than the 27% fertility plateau that is witnessed in IVF when one single embryo is transferred to the uterus,
- Another embodiment of the invention provides a method of targeted intrauterine administration to precisely establish a progesterone dominant uterus within the window of implantation, resulting in a uterus primed and ready to receive a developing blastocyst for implantation.
- This targeted approach mitigates the anatomical and physiological aspects found in prior methods that restrict progesterone reaching the endometrium, while eliminating the excessive buildup that occurs which hinders implantation via conventional methods.
- a hormonal preparation containing a formulation with at least one implantation promoter such as progesterone, estrogen or combinations thereof is delivered to the target site.
- the hormonal preparation is administered in the proper proportions and with the proper timing to optimize and ensure the probability of a successful pregnancy.
- the time period for administration of a hormonal preparation prior to depositing the embryo varies from the same day to 270 days post administration.
- the concentration range of the implantation promoter is between approximately 0.001 micrograms to 1000 milligrams.
- Hormonal preparations may be in a nanoformulation and incorporate a micronized particle mix using micronization methods known in the art such as wet-milling of particles. Excipients can be used which are known to alter the pharmacokinetics and dynamics of hormonal bioavailability.
- the optimum specific time period and hormone concentration range can be determined through routine experimentation wherein the most desirable bioavailability range for promoting the maximum effect of uterine factors necessary for implantation is determined. Because of the direct delivery to the endometrial surface at precisely the right time, timing and concentration can be easily modified for optimum activity of the uterine factors.
- AQPs Aquaporins
- EaCs Epithelial Sodium Channels
- Prostaglandins Activation of the Sodium Pump and HNE-1 Protein for embryonic transport and immobilization
- other genomic factors on the uterine and blastocyst surfaces that require precise hormonal exposure to assure needed dialog between the uterus and blastocyst (termed: maternal dialog), for the continued decidualization process of implantation to occur.
- a still further embodiment of the present invention is where the administration of an implantation promoter to the uterine target site occurs simultaneously with the deposition of the embryo. Administration and deposition occur simultaneously through the same delivery device.
- Depositing the fertilized embryo at the implantation site can occur through any delivery means know in the art. These may include an embryo transfer catheter, syringe, or other ejection device.
- the fertilized embryo can also be deposited directly by manually placement of the embryo.
- a delivery means having a catheter with an outer sheath and an inner lumen slidably disposed within the outer sheath, as described elsewhere herein, the internal lumen has an internal passageway configured to hold an embryo and having a means for quantitative administration of a fluid medium. Accordingly both the embryo and hormonal preparation can be delivered through the same device with the quantitative administration of a fluid medium containing a hormonal preparation having a formulation with at least one implantation promoter.
- This delivery means is ideally suited for the simultaneous delivery of a hormone preparation and the deposition of the fertilized embryo.
- deposition of the fertilized embryo can occur with the precisely-timed delivery of a prior amount of implantation promoter to have the uterus hormonally prepared for implantation.
- delivery of the embryos 72 to the uterus 76 is possible.
- the distal ends of the outer sheath 10 and inner lumen 36 are brought together.
- the physician and/or embryologist directs the end of the ET delivery catheter 6 transvaginally to the desired location within the uterus, either by conventional means or by improved ultrasound guided techniques.
- the gradations 28 provided at the distal end of the outer sheath 10 enable the physician and/or the embryologist to determine the distance the catheter 6 has been inserted into the uterus.
- the ET delivery catheter 6 must be gently advanced through the cervical canal to overcome obstruction or tortuous route so that the delivery catheter 6 can pass into the uterus.
- the physician and/or embryologist then ejects the embryos 72 through the distal end of the inner lumen 36 by depressing the plunger 60 of the syringe 52. In this manner, the entire contents are expelled out of the internal passageway 34. In this manner, the wash fluid 64 washes the wall of the internal passageway 34 and carries out any adherent embryos 72 that may have attached themselves to the wall of the internal passageway 34 by way of surface tension.
- the physician extracts the distal tip of the ET delivery catheter from the cervix. This method of ET is superior to other methods because the delivered embryos are much more likely to flow within a liquid environment than a gaseous (air) environment.
- the catheter as described can be used for intrauterine administration and allows high precision regarding the site for administration, amount of preparation to be administered. Thus, it enables to deliver an implantation promoter at the desired site in the useful amount very precisely.
- the device for delivery is a catheter as defined above and as shown in the figures.
- a delivery catheter for the introduction of one or more media into a uterus of a patient during an embryo transfer procedure wherein said delivery catheter comprises an outer sheath (10) having a proximal end and a distal end; and an inner lumen (36) slidably disposed within said outer sheath (10), said inner lumen (36) having a proximal end, a distal end, and an internal passageway (34) therein for receiving at least one embryo and other media, said inner lumen (36) including at least one visual marker (38) situated on the exterior surface thereof adjacent its distal end indicating a predetermined volume to enable aspiration of an accurate volume of said one or more media into said inner lumen (36), wherein said at least one visual marker (38) comprises a plurality of visual markers (38), each visual marker (38) indicating a volume
- a method for artificial embryonic implantation in a female subject comprising:
- the female subject is human or a mammal, such as a cow or horse.
- the implantation promoter is selected from a group consisting of known amounts of progesterone, estrogen, and a combination thereof.
- uterine factors are selected from the group consisting of aquaporins, epithelial sodium channels, prostaglandins, activation of the sodium pump, HNE-1 protein and combinations thereof.
- the window of implantation is a time period for having the maximum effect on the uterine factors as determined for an individual female subject. 8. The method of paragraph 1 wherein the window of implantation is between approximately the same day as depositing a fertilized embryo to approximately 270 days prior to depositing a fertilized embryo.
- a method for artificial embryonic implantation in a female subject comprising:
- the implantation promoter is selected from a group consisting of known amounts of progesterone, estrogen, and a combination thereof. 15. The method of paragraph 12 wherein the implantation promoter is progesterone.
- uterine factors are selected from the group consisting of aquaporins, epithelial sodium channels, prostaglandins, activation of the sodium pump, HNE-1 protein and combinations thereof.
- a kit for artificial embryonic implantation in a female subject comprising: a. a formulation having at least one implantation promoter in an amount sufficient to promote the activation of uterine factors when administered directly to the uterus; and
- kit of paragraph 19 further having an ejection device selected from a group consisting of an embryo transfer catheter, a syringe, and an ejection device capable of a quantitative administration of a fluid medium.
- Implantation promoter for use in a method for artificial embryonic implantation in a female subject wherein the method comprises
- the implantation promoter for use of paragraph 21 wherein the compound having implantation promoting activity is selected from a group consisting of progesterone, estrogen, and a combination thereof.
- Implantation promoter for use in a method for artificial embryonic implantation in a female subject wherein the method comprises:
- the implantation promoter for use of paragraph 34 wherein the implantation promoter is selected from a group consisting of progesterone, estrogen, and a combination thereof. 39. The implantation promoter of paragraph 34 wherein the implantation promoter is progesterone. The implantation promoter for use of paragraph 34 wherein the concentration of the implantation promoters are between approximately 0.001 micrograms to 1000 milligrams. The implantation promoter for use of paragraph 34 wherein the uterine factors are selected from the group consisting of aquaporins, epithelial sodium channels, prostaglandins, activation of the sodium pump, HNE-1 protein and combinations thereof. The implantation promoter for use of paragraph 34 wherein simultaneous delivery is through an ejection device capable of a quantitative administration of a fluid medium having at least one implantation promoter and a fertilized embryo.
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- Biomedical Technology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Pregnancy & Childbirth (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Transplantation (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Neurosurgery (AREA)
- Dermatology (AREA)
- Urology & Nephrology (AREA)
- Media Introduction/Drainage Providing Device (AREA)
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP18178360 | 2018-06-18 | ||
EP19151230 | 2019-01-10 | ||
PCT/IB2019/000601 WO2019243884A1 (en) | 2018-06-18 | 2019-06-18 | Composition and kit for use in a method of embryo transfer |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3806822A1 true EP3806822A1 (en) | 2021-04-21 |
Family
ID=67544274
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19749780.3A Pending EP3806822A1 (en) | 2018-06-18 | 2019-06-18 | Composition and kit for use in a method of embryo transfer |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP3806822A1 (en) |
WO (1) | WO2019243884A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4265206A1 (en) * | 2022-04-19 | 2023-10-25 | Premium Fertility S.L | System for embryo transfer |
WO2023203052A1 (en) * | 2022-04-19 | 2023-10-26 | Premium Fertility S.L. | System for embryo transfer |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5869081A (en) * | 1996-06-28 | 1999-02-09 | The Population Council | Progesterone vaginal ring for treatment of infertility |
US9107696B2 (en) * | 2008-08-06 | 2015-08-18 | Emory University | Method of embryo transfer that eliminates transferred air while hormonally inducing implantation and apparatus |
-
2019
- 2019-06-18 EP EP19749780.3A patent/EP3806822A1/en active Pending
- 2019-06-18 WO PCT/IB2019/000601 patent/WO2019243884A1/en unknown
Also Published As
Publication number | Publication date |
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WO2019243884A1 (en) | 2019-12-26 |
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