EP3733849A4 - IMPROVED PROMOTER AND USE THEREOF - Google Patents

IMPROVED PROMOTER AND USE THEREOF Download PDF

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Publication number
EP3733849A4
EP3733849A4 EP18893487.1A EP18893487A EP3733849A4 EP 3733849 A4 EP3733849 A4 EP 3733849A4 EP 18893487 A EP18893487 A EP 18893487A EP 3733849 A4 EP3733849 A4 EP 3733849A4
Authority
EP
European Patent Office
Prior art keywords
improved promoter
promoter
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18893487.1A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3733849A1 (en
Inventor
Gaoxu XUE
Tianming QI
Aihua FENG
Zhengli XIE
Yankai JIA
Xin Wu
Zhongping Sun
Guojuan LIAO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genewiz Suzhou Ltd
Original Assignee
Genewiz Suzhou Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genewiz Suzhou Ltd filed Critical Genewiz Suzhou Ltd
Publication of EP3733849A1 publication Critical patent/EP3733849A1/en
Publication of EP3733849A4 publication Critical patent/EP3733849A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP18893487.1A 2017-12-29 2018-12-20 IMPROVED PROMOTER AND USE THEREOF Pending EP3733849A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201711490227.9A CN108118058B (zh) 2017-12-29 2017-12-29 一种改进的启动子及其应用
PCT/CN2018/122309 WO2019128836A1 (zh) 2017-12-29 2018-12-20 一种改进的启动子及其应用

Publications (2)

Publication Number Publication Date
EP3733849A1 EP3733849A1 (en) 2020-11-04
EP3733849A4 true EP3733849A4 (en) 2022-01-12

Family

ID=62232654

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18893487.1A Pending EP3733849A4 (en) 2017-12-29 2018-12-20 IMPROVED PROMOTER AND USE THEREOF

Country Status (4)

Country Link
US (1) US20210032636A1 (zh)
EP (1) EP3733849A4 (zh)
CN (1) CN108118058B (zh)
WO (1) WO2019128836A1 (zh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
CN108165551B (zh) * 2017-12-30 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的t载体和应用
CN108823208B (zh) * 2018-06-29 2021-11-02 中国科学院过程工程研究所 一种四环素诱导启动子及其制备方法和应用
CN111321141B (zh) * 2018-12-14 2021-02-26 上海凯赛生物技术股份有限公司 稳定期特异性启动子及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1564294A1 (en) * 1997-05-07 2005-08-17 Genomics One Corporation Improved cloning vector containing marker inactivation system
WO2015115619A1 (ja) * 2014-01-31 2015-08-06 株式会社カネカ Ｒ体特異的エノイル－ＣｏＡヒドラターゼ遺伝子の発現が調節された微生物及びそれを用いたポリヒドロキシアルカノエート共重合体の製造方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69926009D1 (de) * 1998-10-13 2005-08-04 Univ Georgia Res Found Stabilisierte bioaktive peptide und methoden zu ihrer identifikation, synthese und verwendung
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP1499709B1 (en) * 2002-04-22 2012-01-04 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
CN104513830B (zh) * 2015-01-27 2017-08-11 华东理工大学 一种适用于氧化葡萄糖酸杆菌的基因表达载体及其应用
CN105907632B (zh) * 2016-04-27 2018-01-12 南京巨鲨显示科技有限公司 基因工程生物指示剂
CN107058316A (zh) * 2016-12-09 2017-08-18 江南大学 一种枯草芽孢杆菌自诱导表达系统及其应用
CN106939310B (zh) * 2017-01-24 2020-05-08 湖北大学 一种基于核糖体结合位点改造的启动子优化方法
CN108060168B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的t载体和应用
CN108130338B (zh) * 2017-12-29 2022-02-01 苏州金唯智生物科技有限公司 一种前t载体及其组成的t载体和应用
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108588102B (zh) * 2017-12-29 2021-10-08 苏州金唯智生物科技有限公司 一种前t载体及其组成的t载体和应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
CN108165551B (zh) * 2017-12-30 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的t载体和应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1564294A1 (en) * 1997-05-07 2005-08-17 Genomics One Corporation Improved cloning vector containing marker inactivation system
WO2015115619A1 (ja) * 2014-01-31 2015-08-06 株式会社カネカ Ｒ体特異的エノイル－ＣｏＡヒドラターゼ遺伝子の発現が調節された微生物及びそれを用いたポリヒドロキシアルカノエート共重合体の製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 24 September 2015 (2015-09-24), "Escherichia coli lac promoter, SEQ ID 49.", XP002804923, retrieved from EBI accession no. GSN:BCD14467 Database accession no. BCD14467 *
JAIN ET AL: "New improved lacZ gene fusion vectors", GENE, ELSEVIER AMSTERDAM, NL, vol. 133, no. 1, 29 October 1993 (1993-10-29), pages 99 - 102, XP023540196, ISSN: 0378-1119, [retrieved on 19931029], DOI: 10.1016/0378-1119(93)90231-Q *
KOSTYLEV MAXIM ET AL: "Cloning Should Be Simple: Escherichia coli DH5[alpha]-Mediated Assembly of Multiple DNA Fragments with Short End Homologies", PLOS ONE, vol. 10, no. 9, 8 September 2015 (2015-09-08), pages e0137466, XP055868491, Retrieved from the Internet <URL:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0137466&type=printable> DOI: 10.1371/journal.pone.0137466 *
See also references of WO2019128836A1 *

Also Published As

Publication number Publication date
CN108118058A (zh) 2018-06-05
US20210032636A1 (en) 2021-02-04
CN108118058B (zh) 2021-06-29
EP3733849A1 (en) 2020-11-04
WO2019128836A1 (zh) 2019-07-04

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