EP3687306A1 - Utilisation d'un additif pour ameliorer la qualite de boissons a base de mouts vegetaux - Google Patents
Utilisation d'un additif pour ameliorer la qualite de boissons a base de mouts vegetauxInfo
- Publication number
- EP3687306A1 EP3687306A1 EP18789203.9A EP18789203A EP3687306A1 EP 3687306 A1 EP3687306 A1 EP 3687306A1 EP 18789203 A EP18789203 A EP 18789203A EP 3687306 A1 EP3687306 A1 EP 3687306A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- additive
- cysteine
- bromelain
- wine
- cofactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000654 additive Substances 0.000 title claims abstract description 59
- 230000000996 additive effect Effects 0.000 title claims abstract description 57
- 235000013361 beverage Nutrition 0.000 title claims abstract description 29
- 235000014101 wine Nutrition 0.000 claims abstract description 46
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 40
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 40
- 108010005843 Cysteine Proteases Proteins 0.000 claims abstract description 39
- 102000005927 Cysteine Proteases Human genes 0.000 claims abstract description 39
- 235000018102 proteins Nutrition 0.000 claims abstract description 35
- 235000013405 beer Nutrition 0.000 claims abstract description 23
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 10
- 235000019987 cider Nutrition 0.000 claims abstract description 5
- 239000004365 Protease Substances 0.000 claims description 71
- 108010004032 Bromelains Proteins 0.000 claims description 52
- 235000019835 bromelain Nutrition 0.000 claims description 52
- 235000018417 cysteine Nutrition 0.000 claims description 28
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 28
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 26
- 235000013311 vegetables Nutrition 0.000 claims description 25
- 235000013399 edible fruits Nutrition 0.000 claims description 22
- 235000007119 Ananas comosus Nutrition 0.000 claims description 21
- 244000099147 Ananas comosus Species 0.000 claims description 20
- 235000001014 amino acid Nutrition 0.000 claims description 15
- 239000011593 sulfur Substances 0.000 claims description 15
- 229910052717 sulfur Inorganic materials 0.000 claims description 15
- 108010024636 Glutathione Proteins 0.000 claims description 13
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 13
- 229960003180 glutathione Drugs 0.000 claims description 13
- 229930182817 methionine Natural products 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 150000003464 sulfur compounds Chemical class 0.000 claims description 12
- 230000007065 protein hydrolysis Effects 0.000 claims description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 9
- -1 sulfur amino acid Chemical class 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- 235000008397 ginger Nutrition 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 159000000007 calcium salts Chemical class 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000005352 clarification Methods 0.000 claims description 2
- 244000273928 Zingiber officinale Species 0.000 claims 1
- 239000006174 pH buffer Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 description 25
- 239000000284 extract Substances 0.000 description 23
- 102000035195 Peptidases Human genes 0.000 description 16
- 108091005804 Peptidases Proteins 0.000 description 16
- 230000007062 hydrolysis Effects 0.000 description 11
- 238000006460 hydrolysis reaction Methods 0.000 description 11
- 235000020097 white wine Nutrition 0.000 description 11
- 239000000499 gel Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 241000234314 Zingiber Species 0.000 description 7
- 235000019419 proteases Nutrition 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 239000000440 bentonite Substances 0.000 description 6
- 229910000278 bentonite Inorganic materials 0.000 description 6
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000012429 reaction media Substances 0.000 description 5
- 241000234671 Ananas Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000020708 ginger extract Nutrition 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000029983 protein stabilization Effects 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 241000393496 Electra Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 description 3
- 240000006365 Vitis vinifera Species 0.000 description 3
- 235000014787 Vitis vinifera Nutrition 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 235000020006 fruit beer Nutrition 0.000 description 3
- 235000019990 fruit wine Nutrition 0.000 description 3
- 229940002508 ginger extract Drugs 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 229940013712 pineapple extract Drugs 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 244000291564 Allium cepa Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 240000006432 Carica papaya Species 0.000 description 2
- 235000009467 Carica papaya Nutrition 0.000 description 2
- BUXAPSQPMALTOY-WHFBIAKZSA-N Cys-Glu Chemical compound SC[C@H](N)C(=O)N[C@H](C(O)=O)CCC(O)=O BUXAPSQPMALTOY-WHFBIAKZSA-N 0.000 description 2
- RITKHVBHSGLULN-WHFBIAKZSA-N L-gamma-glutamyl-L-cysteine Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(O)=O RITKHVBHSGLULN-WHFBIAKZSA-N 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 235000013334 alcoholic beverage Nutrition 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
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- 235000013305 food Nutrition 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 108010068906 gamma-glutamylcysteine Proteins 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 230000008092 positive effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 235000009434 Actinidia chinensis Nutrition 0.000 description 1
- 244000298697 Actinidia deliciosa Species 0.000 description 1
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241001507865 Aspergillus fischeri Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 208000025978 Athletic injury Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 108010076667 Caspases Proteins 0.000 description 1
- 102000011727 Caspases Human genes 0.000 description 1
- 102000005600 Cathepsins Human genes 0.000 description 1
- 108010084457 Cathepsins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108090000270 Ficain Proteins 0.000 description 1
- 241000218218 Ficus <angiosperm> Species 0.000 description 1
- 108010002537 Fruit Proteins Proteins 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000795633 Olea <sea slug> Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000235402 Rhizomucor Species 0.000 description 1
- 206010041738 Sports injury Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000019836 ficin Nutrition 0.000 description 1
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 208000001297 phlebitis Diseases 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 201000009890 sinusitis Diseases 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 108090000346 stem bromelain Proteins 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000011514 vinification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/003—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages by a biochemical process
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/30—Removing undesirable substances, e.g. bitter substances
- A23L11/33—Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/62—Clouding agents; Agents to improve the cloud-stability
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22033—Fruit bromelain (3.4.22.33), i.e. juice bromelain
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G2200/00—Special features
- C12G2200/15—Use of particular enzymes in the preparation of wine
Definitions
- the subject of the present invention is an additive for the treatment of drinks based on vegetable musts, in particular wine, beer, cider or fruit juices, and more particularly for improving the quality of such beverages, namely in particular the stability protein and to avoid the disorder of such drinks, generally due to the presence of yeasts, suspended particles, mainly proteins, which can have very different sizes and compositions.
- Field of the invention is an additive for the treatment of drinks based on vegetable musts, in particular wine, beer, cider or fruit juices, and more particularly for improving the quality of such beverages, namely in particular the stability protein and to avoid the disorder of such drinks, generally due to the presence of yeasts, suspended particles, mainly proteins, which can have very different sizes and compositions.
- a known solution is to treat musts and wines with bentonite but the necessary doses according to the needs can be high enough to deplete the organoleptic characteristics of wines thus treated.
- Assays were conducted enzymatically using exogenous proteases or obtained from yeasts, in order to eliminate proteins responsible for protein breakage, but the results were unsatisfactory.
- fungal proteases from sources such as Aspergillus niger, Aspergillus oryzae, Rhizomucor meihei and Neosartorya fischeri;
- yeast proteases from sources such as Candida olea and Saccharomyces cerevisiae; Bacterial proteases of Bacillus subtilis, or Bacillus lichenifomis;
- animal proteases pepsin and trypsin from cattle or pork.
- the invention relates to an additive for improving the quality of vegetable must-based beverages comprising a cysteine protease and a cofactor.
- a first object of the invention relates to the use of an additive comprising a cysteine protease and a cofactor for improving the quality of vegetable musts beverages by increasing proteic hydrolysis.
- the cysteine protease can be of any type: of plant, animal, bacterial or synthetic origin.
- cysteine proteases extracted from various fruits such as pineapple, papaya, fig, kiwi, ginger ... More generally, cysteine proteases include cathepsins, caspases, calpains
- cysteine protease is of plant origin.
- One such protease may be bromelain extracted from pineapple, extracted from pineapple fruit (excluding leaves, stems and roots), or extracted from the stem (leaves, stems and roots).
- bromelain is extracted from the fruit.
- the invention relates to the use of an additive for the protein stabilization of beverages containing vegetable bromelain, characterized in that the bromelain is extracted from the pineapple fruit and in it further contains a sulfur amino acid or a peptide comprising a sulfur amino acid.
- bromelain is also used by the food industry to soften the meat.
- the marketed bromelain is generally derived from pineapple waste, and mainly stems.
- cysteine protease is extracted from ginger.
- the cofactor used to potentiate the activity of the cysteine protease may be a sulfur compound or a calcium salt.
- said amino acid or sulfur peptide is cysteine, methionine, glutathione, N-acethylcysteine, ⁇ -Glu-Cys ( ⁇ -glutamylcysteine) or Cys-Glu (cysteinylglutamic acid).
- the calcium salt is calcium chloride.
- the drink made from vegetable musts can be beer, wine (white, rosé, red), fruit juice, cider ...
- “Improving the quality of vegetable must beverages” means improvements associated with protein hydrolysis, such as improving protein stability (or reducing protein instability), and / or regarding alcoholic beverages, the improvement of alcoholic fermentation.
- the determination of the amount of cofactors present in the vegetable must drink may be carried out either at the time of adding the additive, or beforehand by type of beverage. In the latter case, it is possible to prepare in advance additives adapted to the different types of drinks made from vegetable musts and to add them without a step of dosage of the amount of cofactors.
- the cofactor may be added to the reaction medium according to three (non-limiting) protocols:
- step 1 by addition during the grinding / extraction step as defined in Example 2, step 1; or
- the cofactor is an amino acid or sulfur peptide
- its addition can be carried out in the form of powder or extracts rich in sulfur amino acid (dry yeast bark, cruciferous, onion allies, cabbage, ).
- the cofactor is an amino acid or sulfur peptide
- its presence in the reaction medium can be obtained by conversion or bioconversion in the reaction medium of precursors of said amino acid or sulfur peptide.
- a support such as a resin, bentonite, agar-agar ...
- the inventors have demonstrated a significant influence of the pH of the vegetable must on the activity of the additive.
- the addition of the additive can change the pH of the must.
- the lower the pH of the wine the more the cofactor dose must be increased to optimize protein hydrolysis.
- Those skilled in the art will be able to adapt the dose of cofactor according to the pH of the reaction medium and the must to be treated.
- the pH is advantageously but not limited to between 3 and 4, after adding the additive.
- the pH of the medium will be between 3.2 and 3.8, and even more preferably close to or equal to 3.8 after adding the additive in the wine.
- the pH of the medium should preferably be between 4 and 6 after addition of the additive.
- a second subject of the invention relates to an additive for improving the quality of beverages containing vegetable musts, comprising a cysteine protease and a sulfur compound other than cysteine.
- the sulfur compound is selected from glutathione and methionine.
- a third object of the invention relates to a process for preparing an additive for improving the quality of beverages made from vegetable musts.
- this process comprises the following steps:
- centrifugation at a temperature of between 2 ° C. and 8 ° C. - clarification and ultrafiltration.
- the strength of the buffer is advantageously between 0.05 and 1 mol, and preferably 0.1 mol. The skilled person to determine the optimum strength by routine experiments, by successive tests with various forces.
- the preparation of the additive is carried out in 0.1 M phosphate buffer with 15 mM cysteine and pH 7.
- the additive thus prepared is useful in particular for the protein stabilization of beverages made from vegetable musts or the improvement of the alcoholic fermentation of alcoholic beverages made from vegetable musts.
- a fourth subject of the invention relates to a process for improving the quality of beverages based on vegetable musts by increasing protein hydrolysis, consisting in adding one liter of fruit juice, beer or wine, a dose compatible with the usual doses in oenology and breweries of an additive comprising a cysteine protease and a cofactor.
- This dose may be between 1 mg / L and 10000 mg / L.
- this dose is between 0.5 g / HL and 300 g / HI, or between 5 mg / l and 3000 mg / l.
- it is between 1 mg / l and 100 mg / l.
- the method for improving the quality of must-based beverages by increasing the protein hydrolysis, it consists in adding for a liter of fruit juice, beer or wine, a dose of between 1 mg / ml and 30 mg / ml, preferably between 5 mg / ml and 30 mg / ml, an additive comprising a cysteine protease and a cofactor.
- the method according to the invention is a process for the stabilization of beverages containing vegetable musts by adding, for a liter of fruit juice, beer or wine, a dose of between 1 mg / ml and 30 mg / ml, preferably between 5 mg / ml and 30 mg / ml, of a "crude” or "pre-purified” additive comprising bromelain, characterized in that bromelain is extracted from the fruit of pineapple and in that it further contains a sulfur amino acid or a peptide comprising a sulfur amino acid.
- the sulfur compound may be provided by the pineapple extract which naturally contains it and / or by addition from an external source.
- bromelain extract In general, the more the bromelain extract is purified, the less it contains sulfur compound, especially cysteine and more will be added to prepare the additive. These parameters can easily be adjusted by those skilled in the art.
- Figures 2 and 3 show the variation of the grape proteins of the control as a function of the treatments applied.
- Figures 4, 5 and 6 show the decrease in the amount of wine proteins as a function of the pH in the presence of an additive according to the invention, Figure 4 at pH 3, Figure 5 at pH 3.4 and Figure 6 at pH 3.8.
- EXAMPLE 1 Selection of an Optimal Form of Bromelain
- One aspect of the present invention is the selection of an optimal form of bromelain as a cysteine protease used in the vegetable must beverage additive according to the invention.
- Table 1 below shows the different forms of bromelain (in English cysteine proteinases) from the species Ananas comosus listed by the inventors:
- the bromelain object of the present invention can be extracted from the pineapple fruit.
- the latter nevertheless contains two types of bromelain: mainly the type "bromelain fruit” but also the type "bromelain stem”, much less present.
- EXAMPLE 2 Extraction and pre-purification by filtration and ultrafiltration of the enzymatic activity
- Step 1 grinding and extraction
- Step 2 lyophilization of the aforementioned dry extract
- step 1 and 2 leads to a so-called "raw" extract.
- step 1, 3 and 4 leads to a so-called "pre-purified" extract.
- step 1 where no cysteine was added during the extraction, the ginger extract was obtained using the same protocol as that used to prepare the "raw" extract of bromelain used in Example 3.
- an additive according to the invention may comprise a main active compound consisting of:
- the addition of sulfur amino acid can be carried out in the form of a powder or the addition of extracts rich in sulfur amino acid (dry yeast bark, cruciferous, onion allies, cabbage, etc.).
- FIG. 1 represents the electrophoretic profiles of a sample of white wine, with the profile (1) of the wine without treatment, and the profiles (2 to 4) of the wine after treatment with an additive according to the invention, at concentrations respectively 25.5, 12.5, 5 mg / ml.
- Profile (5) corresponds to the profile of the abovementioned extract, with a concentration of 5 mg / ml in water.
- the profiles were established by electrophoresis equipment EXPERION (trade name).
- the samples treated with the additive according to the invention have the two bands associated with grape proteins (approximately 28 and 33 kDa) and also a band below 25 kDa which corresponds to the molecular mass of bromelain.
- Figure 2 shows the protein profiles with two molecular weights, 28 Kda and 33 Kda, for the first Sauvignon wine at pH 3.34.
- the peaks (11, 12 and 13) quantify the weight protein content of 28Kda respectively of the control without additive, wine at pH 3.34 treated with 25.5 mg / ml of additive and wine treated with 50 mg / ml of additive.
- the peaks (21, 22 and 23) quantify the weight protein content of 33 Kda respectively of the control without additive, wine at pH 3.34 treated with 25.5 mg / ml of additive and wine treated with 50 mg / ml of additive.
- a reduction in the protein content is observed during the addition of the additive, with effects differentiated as a function of the molecular weight.
- Figure 3 shows the protein profiles with two molecular weights, 28 Kda and 33 Kda, for the first Sauvignon wine at pH 3.8.
- the peaks (41, 32 and 33) quantify the weight protein content of 28Kda respectively of the control without additive, the wine at pH 3.8 treated with 25.5 mg / ml of additive and wine treated with 50 mg / ml. ml of additive.
- the peaks (41, 42 and 43) quantify the weight protein content of 33 Kda respectively of the control without additive, wine at pH 3.8 treated with 25.5 mg / ml of additive and wine treated with 50 mg / ml of additive.
- a reduction in the protein content is observed during the addition of the additive, with effects differentiated as a function of molecular weight, more markedly than for wine at pH 3.34.
- Cysteine is known to improve the activity of bromelain.
- the inventors compared the effect of three other cofactors on the activity of bromelain: methionine, glutathione and CaC.
- Table 3 Effect of different cofactors on the activity of bromelain on wine.
- the cofactor CaC has also been tested and shows a positive effect on the hydrolysis of wine proteins even if this result is not presented here.
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Abstract
Description
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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FR1758872A FR3071504B1 (fr) | 2017-09-26 | 2017-09-26 | Produit biologique pour ameliorer la stabilite proteique de boissons a base de mouts vegetaux |
PCT/FR2018/052366 WO2019063936A1 (fr) | 2017-09-26 | 2018-09-26 | Utilisation d'un additif pour ameliorer la qualite de boissons a base de mouts vegetaux |
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EP3687306A1 true EP3687306A1 (fr) | 2020-08-05 |
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EP18789203.9A Pending EP3687306A1 (fr) | 2017-09-26 | 2018-09-26 | Utilisation d'un additif pour ameliorer la qualite de boissons a base de mouts vegetaux |
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US (1) | US20200277556A1 (fr) |
EP (1) | EP3687306A1 (fr) |
FR (1) | FR3071504B1 (fr) |
WO (1) | WO2019063936A1 (fr) |
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FR3083054B1 (fr) * | 2018-06-27 | 2021-09-03 | Oenotropic Innovation | Revelateur d’aromes comprenant un extrait d’ananas et un compose soufre pour preparations contenant des precurseurs aromatiques |
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US20030165592A1 (en) | 2002-02-28 | 2003-09-04 | Daqing Sun | Effective use of protease in winemaking |
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2018
- 2018-09-26 US US16/649,297 patent/US20200277556A1/en not_active Abandoned
- 2018-09-26 EP EP18789203.9A patent/EP3687306A1/fr active Pending
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US20200277556A1 (en) | 2020-09-03 |
FR3071504B1 (fr) | 2021-01-15 |
FR3071504A1 (fr) | 2019-03-29 |
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