EP3684904A1 - Beleuchtung für einen photobioreaktor - Google Patents
Beleuchtung für einen photobioreaktorInfo
- Publication number
- EP3684904A1 EP3684904A1 EP18769120.9A EP18769120A EP3684904A1 EP 3684904 A1 EP3684904 A1 EP 3684904A1 EP 18769120 A EP18769120 A EP 18769120A EP 3684904 A1 EP3684904 A1 EP 3684904A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- container
- light
- medium
- emitting
- partially
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
Definitions
- the present invention relates to a container, in particular a container of a (photo) bioreactor, preferably a disposable bag, which can accommodate and / or comprise one or more illuminants for irradiating a medium, in particular a biological medium with a photoreactive substance ,
- a container in particular a container of a (photo) bioreactor, preferably a disposable bag, which can accommodate and / or comprise one or more illuminants for irradiating a medium, in particular a biological medium with a photoreactive substance .
- the irradiation or the control of the irradiation of the medium on the inside of the container takes place substantially from the outside, the illuminants or phosphors projecting on the inside and irradiating the medium in particular without contact or without contact.
- the phosphors may also be in contact with the medium.
- a photo-bioreactor which is used to store and / or store photoreactive media, for example comprising plants, bacteria, fungi, plant and / or animal cells, living organisms, eukaryotes and / or prokaryotes to process, while these reactions or processes undergo comprehensive photo- or light-induced reactions, such as photosynthesis, or processes, in particular photo-biochemical and / or biotechnological processes.
- photoreactive media for example comprising plants, bacteria, fungi, plant and / or animal cells, living organisms, eukaryotes and / or prokaryotes to process, while these reactions or processes undergo comprehensive photo- or light-induced reactions, such as photosynthesis, or processes, in particular photo-biochemical and / or biotechnological processes.
- PAR photosynthetic active radiation
- the invention relates to a disposable bag or a container of a "single use" / disposable bioreactor, which comprises one or more pockets for receiving one or more bulbs
- the bags which are referred to in the following mainly as bulbs-receiving pockets , are adapted to receive one or more lamps such that they can irradiate on the inside of a medium in the container, in particular a biological medium and thereby a photochemical reaction of light-sensitive or
- a container of a bioreactor in particular a disposable bag, comprise a container casing which at least partially or completely surrounds a container interior.
- a container casing may comprise a container bottom, a container lid, a preferably barrel-shaped peripheral wall / surface and / or a container door.
- Bulb-receiving pockets may be disposed on the container shell.
- the invention also relates to a container which comprises one or more lighting means which can be arranged on the container casing such that an emitted light of the lighting means or of the lighting means reaches the inside of the container in order to irradiate a medium there.
- one or more lamps are arranged on / in a container shell and / or on / in a stirring system and / or on / in a wall of a container door of a container or a container door wall such that they are on the inside a in irradiate the medium located in the container and thereby initiate or initiate a photochemical reaction of the light-sensitive or photosensitive or photoreactive substance.
- the invention can be used in particular in one or more of the following areas: biotechnology, molecular farming, processing of biological / chemical media, phototrophic bioprocessing, biofuels, biorefinery concepts, nutritional supplements, cosmetics, raw material production, food technology, beverage technology, chemical industry, chemical research , Laboratory supplies, medical technology, pharmacy, process chemistry, technical chemistry.
- the invention may relate to photoreactors for large-scale production and / or standard photoreactors for basic research in the laboratory and / or photosynthesis plants and / or plants for the production or cultivation of plants, in particular algae breeding plants or Algae farms and / or for the production or cultivation of bacteria, such as cyanobacteria.
- a photoreactive substance or a photoreactive medium which is located in a container interior volume of a container of a photo-bioreactor, is irradiated from the outside by means of a light source through transparent Benzoiterwand through.
- a light source may, for example, be the sun, whose radiation spectrum on the earth is particularly suitable for irradiating plants and / or algae which carry out photosynthesis.
- lamps such as aquarium lamps or other lamps, which have an emission spectrum similar to that of the sun, can be used for the artificial irradiation of plants and / or algae.
- a photo-synthetic active illumination is used.
- a medium is usually passed through transparent tubes and irradiated from the outside with light.
- containers or vessels such as laboratory bottles on a scale of a few liters with transparent glass wall, for example, artificially irradiated from the outside. Due to the fact that the bottles have only a small volume, a light emitted from the outside, which is irradiated, for example, from one side, can still be absorbed by a medium even on the inside of the container opposite the direction of irradiation.
- the radiation power and / or the density of the absorbing medium and / or the number of lamps used and / or the size or the volume of the vessel are usually adjusted.
- the invention relates to a container, in particular a disposable bag, and preferably a container / disposable bag of a bioreactor and / or a mixing tank or mixing container with a container interior or volume, designed for at least partial filling or filling or Filling with a medium comprising at least one photoreactive material for at least partially triggering or initiating at least one photochemical reaction of the photoreactive (chemical and / or biological) substance or a chemical by irradiation with light or electromagnetic radiation, comprising:
- a container shell which surrounds the container interior and at least one Leuchtm ittelöff n ung for introducing at least one light source through the container shell in a region of the container interior has; and at least one light-emitting means receiving pocket which is transparent at least in part to an electromagnetic radiation, preferably a multiplicity of light-receiving pockets which are arranged on the at least one light-emitting means opening at least partially within the container interior and are or are designed to have at least one light-emitting means.
- the lighting means or the lighting means which is or are isolated from the medium by the receiving pocket (s) is not in direct contact with the medium, which on the one hand protects the medium from external contamination, However, at the same time allows irradiation of the medium with emitted light of at least one light source.
- a container in particular a disposable bag, is designed to be filled at least partially with a medium comprising at least one photoreactive substance suitable for at least partially triggering at least one photochemical reaction of the photoreactive or photochemical substance by irradiation with electromagnetic Radiation comprising:
- At least one illuminant receiving pocket which is transparent at least partially to an electromagnetic radiation and which is arranged or fastened or attached at least partially within the container interior to a luminous means opening of the container envelope and is designed to at least ; a light source or a plurality of bulbs from the outside through the bulb opening at least partially receive, wherein the bulb receiving pocket (s) and / or the ' bulb opening (s) is / are designed so that the medium at least partially so by means of the / Illuminant can be irradiated that the at least one photochemical reaction of the at least one photoreactive material can be triggered by means of an emitted from the light source or the bulbs electromagnetic radiation and the or the light source is isolated by the Leuchtffenabilitytaschein) to the medium.
- the invention is essentially one Container, which is preferably a disposable container, in particular a disposable bag, but may also be a reusable container.
- the container comprises a receiving pocket or a plurality of receiving pockets or pockets or bulbs receiving pockets which is designed to receive one or more light sources and / or light sources.
- the light-receiving pockets may protrude substantially on the inside of the container or in the container interior, in particular when the light-receiving pockets have accommodated lighting means.
- the container may also be, for example, a canister, a steel tank, a barrel or other vessel.
- a disposable bag may be disposed within a bioreactor.
- a medium in the container interior can be irradiated by the emitted light of the at least one Leuchtmitteis.
- the medium comprises a photosensitive or reactive substance, for example an alga
- a process or a reaction can be initiated or triggered by means of the light irradiation of the illuminant in the switched on or switched-on state or during operation of the illuminant. be initiated.
- the algae can be made to operate photosynthesis.
- irradiation of the photosensitive or reactive substance provides energy in the form of photons and at least partially absorbs or accommodates it through the photosensitive or reactive substance, so that one or more photochemical and / or biochemical and / or physical reactions to be triggered.
- the light-emitting means receiving pocket is at least partially transparent, in particular for light of the visible wavelength range, a light emitted by a light source can at least partially pass through the wall of the light-receiving pocket or through the (wall-mounted) pocket wall propagate through and thus irradiate the located in a container interior volume or medium.
- a light source is at least partially received by a light bulb receiving pocket
- the light source is preferably located substantially on the inside of the tank or in the interior of the tank. Nevertheless, the light source is through the wall of the bulb-receiving pocket or by the (illuminant- Receiving) pocket wall of the medium or from the container interior isolated so that there is essentially no direct contact between the medium and the bulb.
- an "exclusive container interior” may be defined, which is characterized by containing only the volume of a medium. "Exclusive container interior” does not include the volume containing one or more bulbs and bulb receiving pockets occupy, in particular if the at least one light-emitting receiving pocket has at least partially received at least one light-emitting means.
- the container interior or container volume should be defined such that it would correspond to the volume that the container would have if it did not comprise a light-emitting receiving pocket (s) and instead a substantially flat container sleeve. that is, not indented by a bulb receiving bag or curved inwards.
- a container interior corresponds to a volume of a container without bulbs on receiving pocket (s), in particular if the container shell comprises no bulbs for bulbs receiving pockets.
- a light source if it is at least partially accommodated by a light bulb receiving pocket or at least partially disposed within a light bulb receiving pocket, at least partially in the container interior, but not located substantially in the exclusive container interior ,
- the light-emitting means is finally isolated from the medium by the pocket wall or the wall of the light-receiving pocket of the medium or isolated.
- a container may include a bulb receptacle or about two, three, four, five, six, seven, and most preferably up to about thirty bulb receiving pockets. However, it is not excluded that a container comprises more than thirty light bulb receiving pockets.
- a light-receiving pocket for example, record several bulbs and / or light sources.
- a light-emitting receiving pocket can accommodate approximately two, three, four, five, six, seven and more light sources, in particular a bundle of light sources, for example optical waveguides.
- illuminants can also be added, exchanged and / or removed from outside during a chemical process, so that the irradiation or lighting situation can be adapted to the respective process, without the container necessarily having to be opened for access from outside, which could affect the process flow.
- the container interior is accessible from outside through the light-receiving pockets, but nevertheless is insulated from the outside by the pocket wall of the light-receiving pockets, the lighting situation can be changed without the container interior being opened to the outside must, which could lead to an impairment of the process of a medium on the inside of the container every time.
- physical parameters of the medium or of the container interior are impaired, such as by a temperature change, a pressure change, loss of a substance by evaporation, contamination by substances, for example bacteria, chemicals, oxygen, etc.
- the adaptation of a lighting or irradiation situation can be carried out in a simple manner in that bulbs can be exchanged, added or removed from them by the externally accessible light-receiving pockets.
- a container comprises a lamp receiving pocket or a plurality of lamp receiving pockets, wherein at least one lamp receiving pocket is rigid and / or has a substantially constant volume or pocket internal volume.
- one or more light receiving pockets is or are provided on a container or on a container casing, which essentially can not be compressed or compressed or squeezed together, in particular when the container is being filled with a medium.
- an at least partially rigid training or by a substantially constant volume or pocket inner volume of the bulb receiving pocket can be at least partially prevented that a bulb receiving pocket is compressed or squeezed together by the medium located in the container interior, if no Illuminant is arranged in a pocket interior volume.
- a constant volume or inner volume of the pocket can be provided or made possible by arranging a framework and / or basket-like framework or braid on the light-emitting means receiving pocket and / or in the inner pocket volume and / or the (light-receiving means -) pocket wall of the bulbs receiving pocket reinforcements or thickening of the wall thickness and / or additional materials includes.
- the means by which a substantially constant volume can be achieved is so stable that it can not be deformed or pressed under the influence or under the pressure of the medium located in the container interior.
- a container comprises a bulbs receiving pocket or a plurality of bulbs receiving pockets, wherein the bulbs receiving pocket (s) arranged on one or more illuminant openings is / are, which is positioned on a peripheral wall / surface of the container is / are.
- a container sleeve in particular a circumferential wall, comprises illuminant openings, in particular at a plurality of locations and preferably distributed along a circumference, through which illuminating means and / or light sources can be received from outside through the illuminant receiving pocket (s).
- the container casing of a container comprises a container wall, preferably a barrel-shaped peripheral wall, a container bottom and a container lid which can be removed from the container wall, wherein the at least one illuminant opening is formed in the container lid.
- a container wall, in particular a preferably barrel-shaped peripheral wall of the container casing, and / or a container lid of the Be Strukturerhülie each openings or illuminant openings comprise can be arranged by which lighting means in a light-receiving pocket or inserted into a light-receiving pocket.
- the container is a disposable container, particularly a disposable container of a bioreactor.
- a disposable element such as a disposable container, in particular a disposable bag, has the general advantage that this and in particular its interior can be provided sterile and does not need to be re-cleaned or sterilized or autoclaved after use and contamination with contents but can be disposed of.
- inexpensive materials for the production of disposable bioreactors processes can be carried out or implemented particularly cost-effective.
- It may be formed as disposable items, all components of a container, in particular a bioreactor, as well as "all accessories.
- a light-receiving pocket may be formed at least partially from a plastic, in particular from a soft plastic and / or sections of a metal, such as steel, attached and / or fixed and / or glued and / or welded.
- An illuminant receiving pocket may comprise a metal, in particular, if the pocket is substantially structurally supported by a metal structure, for example a basket-like braid. should be kept. In this way, it can be avoided that a bulbs receiving pocket, for example, deformed in the unfilled state.
- a light bulb receiving pocket and a container or disposable bioreactor may be multi-piece, in particular two-piece or formed in two pieces and be connected, for example via a composite material.
- bulbs receiving bag and container or disposable bioreactor may be integrally formed.
- a preferred embodiment of a container, in particular a disposable bioreactor, for disposable use may comprise a container wall, bulbs receiving pockets or other elements which substantially or at least partially from a so-called "soft plastic” or from a particularly flexible plastic,
- sections which serve to stabilize the container structure may be formed of a harder material, such elements and / or sections may in part consist of a so-called "hard plastic” or of a hard plastic a stiffer or dimensionally stable plastic, in particular of a (fusible) thermoplastic or of a (non-meltable) thermoset, for example, a synthetic resin to be formed.
- the container is a reusable container, in particular a reusable container of a reusable bioreactor.
- a particularly large amount of a medium for example more When about 500 l, in particular more than about 5000 l, is to be processed and / or stored and / or transported in a container, it is advantageous to use a particularly large container, for example a steel tank.
- a particularly large container for example a steel tank.
- Such containers may prove to be particularly cost effective in reusable use, for example, as reusable bioreactors and / or reusable fermenters and / or reusable mixing systems and / or reusable brewing vessels and / or reusable fermentation systems.
- a bulbs receiving pocket attached and / or screwed and / or fixed and / or glued and / or welded.
- the bulbs receiving pocket may be at least partially formed from a glass and / or a plastic and / or sections of a metal.
- Such bulbs-receiving bag and container or reusable bioreactor can be made in several pieces, in particular two-piece and connected via a composite fabric and / or other means.
- bulbs receiving bag and container or reusable bioreactor can be integrally formed. It may also be that the wall of a reusable container per se comprises only one opening, on which a light-emitting receiving pocket can be arranged or fixed or fastened or fastened.
- a reusable container could be substantially formed of steel, whereas bulb receiving bags, which may for example be single use and sterilizable from a plastic, may be attached to openings of the reusable container.
- a bulb receiving bag may be a hollow and substantially transparent tube, which may be designed for multiple use and may be sterilizable.
- the illuminant receiving pocket (s) is or are configured to have one or more illuminants! comprising a light-emitting rod, a light-emitting fiber or an optical waveguide, a light-emitting diode and / or a light source, in particular a lamp and preferably a tubular lamp and / or a laser from the outside through the Bulb opening at least partially record.
- one of the light bulbs receiving pockets of each of the aforementioned bulbs and / or light sources can accommodate a plurality of said bulbs and / or light sources.
- a light-emitting rod or a light-emitting rod and an optical waveguide can each be received by the container by means of one or more light-emitting means receiving pocket (s).
- the light source a light emitting fiber or a light guide comprise what or which is wound around a member such as a rod and / or a conduit, particularly a conduit for gas supply and / or removal and / or a so-called sparger or tortuous.
- the element, for example the gas line, which is wrapped with the light-emitting fiber 15b may already be located in the interior of the container or be arranged in the interior.
- the wrapped element may also be slid into and / or through an aperture in, for example, a bulb receiving bag, such that when a light is coupled into the fiber, the light is emitted at least partially along the surface of the member towards the inside of the container ,
- the bulbs-receiving pockets which are formed substantially completely waterproof, it can be prevented that bulbs are damaged, which are not usually used in connection with liquids or under water or within liquids.
- An advantage therefore exists in that the aforementioned light sources and / or light sources can essentially pass into a container interior filled with a medium, without these illuminants and / or light sources having to be impermeably watertight. This is particularly advantageous when the medium is a liquid. There is therefore ideally no need for the illuminant! especially suitable for applications under water or in liquids.
- the at least one light-emitting means receiving pocket is made of a same material as a majority of the container cover, in particular made of a transparent soft plastic, and preferably integrally with the Container shell or the container formed.
- the illuminant-receiving pocket and / or the container envelope may also be at least partially formed of glass and / or a transparent hard plastic or resin.
- the container envelope and / or the bulbs receiving pocket can at least partially consist of a substantially transparent glass, for example quartz glass, of a substantially transparent plastic, in particular of an acrylic glass (polymethylmethacrylate, PMMA), a polycarbonate (PC), a polyvinyl chloride ( PVC), a polystyrene (PS), a Poiyphenylenether (PPO), a silicone, in particular a highly transparent silicone and / or a polyethylene (PE), in particular HDPE or LDPE be formed.
- a substantially transparent glass for example quartz glass
- a substantially transparent plastic in particular of an acrylic glass (polymethylmethacrylate, PMMA), a polycarbonate (PC), a polyvinyl chloride ( PVC), a polystyrene (PS), a Poiyphenylenether (PPO), a silicone, in particular a highly transparent silicone and / or a polyethylene (PE), in particular HDPE or LDPE be formed.
- PMMA acrylic glass
- PC
- a container shell likewise from a transparent material, since in this way also a light from the outside, for example sunlight, can at least partially pass through the container envelope or propagate.
- a luminous means or a light source for irradiating the medium in the container interior may be additionally arranged or arranged on the outside from the outside through the transparent container casing.
- the container interior is optimally irradiated or illuminated. This means that the medium is preferably irradiated optimally and / or equally or evenly at all positions in the container interior.
- a medium per se can be substantially turbid or intransparent or can strongly absorb light of a certain wavelength range, it is advantageous to mount light sources or illuminants at different positions inside and possibly also outside the container in such a way that the light passes through the transparent Can pass through container shell and / or walls and / or elements or propagate through.
- the container comprises a Leuchtmittei-receiving pocket, which at least partially filter and / or absorb an electromagnetic radiation, in particular a heat radiation or an infrared radiation and / or can dissipate.
- the filtering of heat radiation which can be absorbed by the medium is advantageous because it can be avoided so that a medium is heated too much by the irradiation, if this should be avoided. Alternatively, this can also be done by discharging a heating air. It is also possible for a light of another frequency range to be at least partially filtered or absorbed by the light-emitting means receiving pocket. This could also be at least partially prevented that excessive UV radiation enters the container interior.
- the container and / or the bioreactor is at least partially, in particular completely sterilizable.
- the container and the bulb receiving pocket are formed from one or more materials which are sterilizable or autoclavable.
- the container and / or the bulbs receiving pocket can be formed from a polymer. This has the advantage that the container together with the bulb receiving pocket can be sterilized before use, so that a medium which is filled, for example, in the container interior of the container in particular is not contaminated with microbiological material.
- the sterilization can be done before using a disposable container, especially a disposable bioreactor. Sterilization may also be done prior to use (and possibly after or between two uses) of a bioreactor.
- the plastic from which, for example, a disposable container is substantially formed, as well as all other elements are largely sterilizable, e.g. using beta or gamma radiation.
- the material used to make a container or to make a bioreactor can be sterilized by thermal sterilization, steam sterilization, hot air sterilization, chemical and / or physical sterilization (e.g., beta or gamma irradiation).
- the invention further relates to a container, in particular according to one or A plurality of preceding aspects, comprising a container interior adapted to be filled with a medium comprising at least one photoreactive agent, and configured to at least partially initiate at least one photochemical reaction of the photoreactive agent, comprising:
- a stirring element comprising a stirring shaft with a stirring shaft cavity and an at least partially transparent stirring shaft wall, wherein the stirring shaft cavity can receive a lighting means and the stirring shaft wall is designed so that the lighting means forms an electromagnetic radiation through the stirring shaft wall emit the container interior and can trigger the photochemical reaction of the photoreactive material of the medium.
- a container which is preferably a disposable bag, comprises a stirring element or stirring device, which comprises a stirring shaft.
- the stirring shaft has a stirring shaft cavity and a substantially or at least partially transparent stirring shaft wall.
- the stirring shaft could also be referred to as a transparent tube.
- a light source in particular a light-emitting rod can be arranged.
- a light emitted from the lighting means can propagate through the transparent stirring wave wall, so that the container interior and the medium therein are irradiated with the photo-reactive substance.
- the stirrer shaft cavity is designed so that a luminous means can be substantially arranged, exchanged and / or removed from the outside within the stirrer shaft cavity.
- a stirring shaft can be used particularly efficiently.
- the stirring shaft serves for mixing or mixing of the medium
- the stirring shaft with the stirring shaft cavity also serves to receive a light source.
- a stirring shaft is preferably arranged centrally in a container, a medium can therefore be irradiated centrally or from the inside of the container or from the center of the container interior volume without having to provide an additional surface for attaching the illuminant.
- a container or bioreactor or mixing tank which is particularly efficient with regard to the irradiation of a medium can also comprise a light source or a light source in the center of the container interior, in this case within a stirring shaft.
- the irradiation is particularly efficient when a flow profile of the medium past a light source past a container envelope and a light source in the center of the container interior.
- a trajectory of a photosensitive molecule is on average such that a molecule is irradiated as optimally as possible with respect to the irradiation duration and intensity of a luminous means, so that within a certain, especially within a short time, the required light is absorbed and a phochemical reaction takes place.
- a lighting means can be arranged, exchanged and / or removed from the outside substantially within the stirring shaft cavity.
- a light source can be exchanged or removed, for example, if a process has largely taken place or ended and the lighting situation is to be adapted.
- a reaction following the first reaction may require irradiation with light of a different frequency spectrum. This could then be solved by the exchange of light sources.
- a container may comprise a transparent stirrer shaft with stirrer shaft cavity for receiving a lamp and one or more illuminant receptacles.
- the features described below are also combinable with the preceding features unless they are mutually exclusive.
- a container having a container interior which is designed to be filled with a medium comprising at least one photoreactive substance, is designed to at least partially trigger at least one photochemical reaction of the photoreactive substance and comprises the following elements:
- a scrubber comprising at least a rotor blade and a stirring shaft
- At least one light source which is at least partially disposed on or in the container inner wall, the stirring shaft and / or the rotor blade and is adapted to at least partially irradiate the medium so that the photochemical reaction of the photoreactive material by means of one of the lighting means emitted electromagnetic radiation can be triggered.
- the container may in particular be combined with the preceding aspects and features of another embodiment of a container.
- At least one container may comprise lighting means and / or light sources, which is arranged or can be arranged on at least one component or element of the container.
- an optical fiber may be wound around a stirring shaft so that a light can be emitted into the container interior along the stirring shaft.
- a container inner wall or a container envelope inner surface can be at least partially or completely provided with a lighting means.
- a wall may comprise light-emitting elements or light sources, in particular organic light-emitting diodes (OLED) or an OLED field, which may or may not be disposed over a large area on the wall.
- OLED organic light-emitting diodes
- individual light emitting diodes may be attached to the container shell.
- glow sticks may be attached to the container shell.
- optical waveguides can also be mounted inside or on the container casing, wherein these optical fibers can emit a light which enters or propagates into the container interior, for example, through a transparent container inner wall or container casing inner surface.
- these optional features have the advantage that a medium in a container interior can be irradiated as large as possible. This may also be the case if the container casing is not transparent, for example.
- All mentioned bulbs can be arranged for example in an alternative or additional aspect in or on a rotor blade.
- particles and / or stirring elements such as, for example, agitated stirrers or stirring fish, which have fluorescent properties, can also be provided in the container, so that a light can also be transmitted to the medium in this way.
- the advantage here is that by stirring the medium, the stirring elements are swirled with the medium and thus in the container interior can cover a distance in different regions of the container interior, whereby the highest possible proportion of the medium can be irradiated.
- such particles or stirring elements can also be equipped with a phosphorescent substance, in particular coated. In that case, the particles may emit a light, in particular after the irradiation of the phosphorescent substance with one of the aforementioned light-emitting elements.
- a medium in the container interior is stirred in such a way that the medium is irradiated as intensively as possible or by the light-emitting elements.
- a portion of the medium may first be irradiated by a light-emitting element on the container shell inner surface and subsequently by stirring reach into an area where it is irradiated by light-emitting elements of a stirring shaft.
- a photoreactive molecule in a medium is irradiated so long and intensively (in one piece or without interruption) on average by one or more light-emitting elements that a photochemical reaction is triggered or promoted or initiated ,
- the container comprises a light-emitting means and / or a light-generating means, wherein a light-emitting means a light-emitting rod, a light-emitting fiber or a light-emitting diode and / or a light source, in particular a lamp and preferably a tubular lamp.
- a light-emitting means a light-emitting rod, a light-emitting fiber or a light-emitting diode and / or a light source, in particular a lamp and preferably a tubular lamp.
- an intensity of the irradiation or an amount of the emitted light or a power of the individual light-emitting elements can be varied or adjusted as required.
- some or a portion of the light-emitting elements can be turned on or off, while another part accordingly turns off or on.
- the irradiation of the medium can also be pulsed or stroboscopic. In particular, however, the medium is irradiated over a longer period at a time or in a longer interval or permanently in order to trigger the highest possible reaction rate or as many photochemical reactions.
- the container inner wall or inner container surface comprises a container inner wall structuring, which is designed to form an enlarged surface with respect to an unstructured, smooth container casing inner surface, and wherein the illuminant is attached to or in the container inner wall.
- Structuring is arranged and / or the container inner wall structuring can influence or predetermine a flow profile of the medium.
- a flow profile of the medium occurs, in particular, when a medium in a container is stirred or mixed by means of a stirring device.
- a particularly large area can be provided on the container inside, as many as possible or as large as possible light Emissive elements can be attached.
- a light-emitting element which emits light over a particularly large area, for example a pedestal or array or array, in particular a light-emitting film comprising one or more OLED elements, can be attached to the structured container inner wall.
- a structured container shell inner surface may cause turbulent flows of the agitated medium, especially in the immediate vicinity of the structured container shell inner surface.
- Turbulent flows may cause part of the media to be in the vicinity of a light-emitting container shell inner surface for a longer time and to flow past the container shell inner surface several times, for example, through a circular airfoil.
- This has the advantage that a container envelope inner surface or a portion of the structured container envelope inner surface, which comprises a light source, can substantially irradiate a specific part of the medium over a prolonged period of time (and / or repeatedly). As a result, the probability of a photochemical reaction of a photosensitive substance in the medium increases.
- a structured Be Zellerhüllen- inner surface comprising a light source causes a certain advantageous flow profile of the medium or causes or causes.
- a certain flow profile of the medium would be advantageous if the flow rate leads to a certain part of the medium being located, if possible, in the proximity of a light source over an optimum period for triggering a photochemical reaction.
- the medium in the tank or container should be well mixed or stirred so that the largest possible volume of the medium, in particular the entire volume or the highest possible number of photoreactive molecules of the medium is irradiated by one or more Leuchtmittein long enough, so the desired photochemical reaction of as many molecules as possible is triggered.
- a certain part of the "Medium” is not a closed size because the part of the medium undergoes essentially continuous mixing with other parts or volumes of the medium during flow, meaning, for example, that within a turbulent flow, several molecules on average are simultaneously somewhat stay longer within a certain volume than in a laminar flow, since the flow essentially flows in a circle or the flow profile is substantially circular.
- irradiation intensity depends on the power of a lamp and the distance between the molecule and Leuchtiittei at which a light of a certain power is emitted. Furthermore, the irradiation intensity also depends on how dense the medium is or how many absorbing molecules are present in the medium. In the event that the medium is very dense, less light may reach more distant areas of the container interior, so that a molecule may only be exposed to sufficient radiation if it is close enough to a light source long enough.
- a container in particular a mixing tank or mixing container
- a container which is designed to as many molecules of a photoreactive material as efficiently as possible, i. to irradiate within the required or optimum duration of irradiation such that the photochemical reaction is triggered.
- a flow profile in conjunction with a lighting situation position of the light sources / light sources, radiation power, absorption and density of a medium
- a stirring device or mixing device optimized to this effect are.
- the optical density in particular the extinction, comprising absorption and scattering represent physical quantities on which it can depend how efficiently an irradiation of light with respect to the reaction rate precipitates.
- a very (visually) dense Medium which strongly absorbs, in particular, a light of at least a part of the frequency range can therefore strongly filter or absorb a color or a frequency range of the light within a short distance of the traveled light. Therefore, a molecule at a greater distance from the light-emitting means or the light-emitting means can no longer be sufficiently irradiated with the light of a specific frequency or wavelength which the molecule would require, so that a photochemical reaction can be triggered.
- a mixing device which is designed so that the molecules also come closer to the light-emitting means in the vicinity in order to be sufficiently strong and laser-irradiated there. It may also be advantageous in this case to adjust the position, as well as the power and the number of light-emitting means, as well as the stirring or mixing speed of the medium.
- the medium can also be thinned so that fewer molecules are present in a container and therefore less light is absorbed in a short distance to a luminous means.
- a container comprises a tube that is transparent or transparent to electromagnetic radiation and that is designed to store and / or guide a medium, wherein the medium can be irradiated, for example, from outside through a transparent tube wall.
- a medium can flow through a transparent tube of a container, wherein the medium can be irradiated from outside through the transparent or transparent tube wall by a light source.
- the medium in the tube can be irradiated from the outside by the sun.
- a tube could be, for example, an inlet and / or a drain and / or a bypass of the container or the bioreactor / represent.
- the invention relates to a method for at least partially initiating or initiating or initiating at least one photochemical reaction of a photoreactive (chemical and / or biological) substance in a medium comprising the following steps:
- a container interior of a container in particular a container of a bioreactor and preferably a disposable bag with the medium which comprises the photoreactive (chemical and / or biological) substance;
- the medium at least partially irradiating or illuminating or illuminating the medium with the luminous means such that the photochemical reaction of the photoreactive substance is at least partially triggered by means of an electromagnetic radiation emitted by the illuminant.
- the invention also relates to a method for at least partially triggering at least one photochemical reaction of a photoreactive substance in a medium, the following steps:
- At least one scrubbing element comprising at least one stirring shaft with a stirring shaft cavity and an at least partially transparent stirring shaft wall at least partially within the container interior;
- a light source or a light source is received by a light-receiving pocket, so that the light source is at least partially within the substantially not yet filled container interior.
- the container interior can preferably be filled with a medium. If you first fill the container interior with a medium, so it could make it difficult to arrange a light source in the bulb-receiving pocket, since the bulb receiving pocket could be squeezed or squeezed by the medium, so when pushing the bulb in the Leuchtmittei-Avem Spotify the wall of the bulb receiving bag could be injured or damaged. However, it is not excluded that a process in the latter order takes place.
- FIG. 1 is a schematic side view with insight into a container of a bioreactor with mixing device and lamp receiving pockets on the
- Container shell according to an embodiment
- Fig. 2a is a schematic exploded view with insight into a container of a bioreactor with mixing device, Benzoiterdecke! and bulb receiving pockets on the container lid according to another embodiment;
- FIG. 2b shows four schematic views from above onto the container lid of a container of a bioreactor with mixing device, container lid and lamp receiving pockets on the container lid according to further embodiments;
- FIG. 3 shows a schematic side view with an insight into a disposable container of a bioreactor or disposable bag with mixing device and light guide on the stirring shaft according to a further embodiment
- Fig. 4 is a schematic side view with insight into a tank or container of a
- Bioreactor with multiple light guides as an exemplary embodiment
- FIG. 5 is a schematic exploded view with an insight into a tank or
- Container of a bioreactor with container lid, bulbs-receiving pockets at the
- 6a is a perspective side view of a portion of a container shell with door according to another embodiment
- 6b is a top or bottom view of a portion of a container shell cross-section with container inner wall structuring and lighting means according to another embodiment.
- FIG. 7 shows a cross-sectional view of a container of a bioreactor with mixing device and various light sources according to a further embodiment
- FIG. 8a is a frontal view of a steel shell bioreactor including a disposable bag in its interior volume according to another embodiment
- Fig. 8b is a front view of an open bioreactor Stahlhülie having a
- Disposable bag visible in its internal volume includes according to the
- FIG. 8c is a frontal view of a disposable bag that may be received by the steel shell bioreactor of the embodiment of FIGS. 8a and b.
- FIG 9a shows a schematic side view with an insight into a disposable container of a bioreactor with mixing device and light rods according to a further embodiment;
- Flg. 9b shows three light sticks according to a further embodiment
- 10a is a schematic side view with insight into a disposable container of a
- Bioreactor with mixing device and light rods with light guides according to a
- Fig. 10b shows a mounting device for optical fibers according to an embodiment
- Fig. 10c light rods with optical fibers according to one embodiment.
- FIG. 1 shows a side view of an embodiment of a container of a bioreactor 1a comprising a container 1 with a container bottom, an upper edge O and a container shell 4. Further, the container of the bioreactor 1a has one or more illuminant openings 12, in particular one or more illuminant openings (FIG. en) in the container wall 12a, and one or more light receiving pockets 11 on. Since the container of the bioreactor 1a is designed to receive one or more lamps 15, the bioreactor 1a can also be referred to as a photo-bioreactor. A lighting means 15 may also be referred to as a light-emitting means or a light-emitting element.
- the bioreactor 1 a further comprises a drive 10 which has a motor, for example a three-phase motor, a stepping motor, a pneumatically driven motor and / or the like.
- the mixing system which is arranged in the container 1, has a mixing device, which is designed and intended to at least partially mix a medium 8 arranged or filled in the container 1.
- the medium 8 may comprise a fluid and / or a solid and / or a gas and may in particular be formed as a fluid mixture and / or a solid mixture, or also as a mixture of at least one fluid and at least one solid.
- a medium 8 may in particular be a biological medium or a chemical medium.
- two illuminating means 15 are partially completely received by the illuminating means receiving pockets 11 on the left side L, and two illuminating means 15 are substantially completely accommodated on the right side R.
- the lighting means 15 are formed as light-emitting rods or elements 15a which can emit an electromagnetic radiation 14 or a light, in particular in the switched-on state.
- FIG. 1 is to be understood as meaning that all or only some of the light-emitting rods 15 a are in each case received completely or partially or not at all by the light-emitting means receiving pockets 11. Also, the embodiment of Fig. 1 is to be understood that the container 1 and the container interior 22 unfilled or at least partially or completely with a medium 8 can be filled / filled. In other words, this embodiment essentially relates to the structural characteristics of the container 1 and the bioreactor 1a, respectively, whereas the light-emitting rods 15a can only be attached to the container 1 and / or a medium 8 can be filled into the container 1 ,
- the two light-receiving pockets 11 on the left side L have only partly received the respective two light-emitting rods 15a, and therefore, since they are not substantially (completely) filled with the light-emitting rods 15a, hang down toward the container bottom V. This condition may be considered as the "unfilled condition" of the bulb receiving pockets 11.
- the bulbs-receiving pockets 11 and their (bulb receiving) pocket walls which is also referred to as pocket walls 11a, may be formed of a soft, flexible and / or possibly of a stretchable plastic. According to Fig. 1, the light-emitting rods 15a on the left side L from the outside A in the pocket inner volume V of the pocket inside T of the bulb receiving pockets 1 1, which on the container inside I and the container interior 22 are used or pushed.
- the two light-receiving pockets 11 on the right side R have the two respective light-emitting rods 15 a substantially completely absorbed, which is why they are substantially perpendicular to the Be bachelorerhüile 4 and horizontally on the inside I and in the container interior 22 of the Container 1 protrude.
- This state may be regarded as the "filled state" of the bulb receiving pockets 11 because the pocket internal volume V on the pocket inner side T of the bulb receiving pockets 11 is substantially completely filled by the light emitting rods 15a.
- the respective light-emitting rod 15a on the right side R from the outside A via the respective bulb opening in the tank wall 12a into the pocket interior volume V of the pocket inside T of the respective bulb receiving pocket 11, which is located on the In this state, the respective light-emitting rod 15a on the container inner side I or in the container interior 22 at least partially emit a light or an electromagnetic radiation 14, wherein the respective light-emitting rod 15 a only du A pocket wall 11a (also referred to as a pocket wall 11a) is essentially isolated from the medium 8.
- illuminant receiving pockets 11, illuminating openings in the container wall 12a, and light emitting bars 15a are respectively provided.
- only one light-emitting means receiving pockets 11 and one light-emitting opening in the container wall 12a or any other number of light-emitting means receiving pockets 11 and illuminant openings may be provided in the container wall 12a.
- another light-emitting means 15, for example an optical waveguide or a lamp, instead of a light-emitting rod 15a can also be accommodated by the light-emitting means receiving pockets 11.
- the light-emitting rods 15a for example, in the on state or in operation by battery or battery and / or via a line 13, in particular via a power cord with power plug with energy or power or voltage can be supplied.
- the container 1 of the bioreactor 1 a is in the embodiment shown a tank, which may be a steel tank, for example, and is penetrated by a stirring element 3, which is arranged on the container inside I of the container 1 and can completely penetrate the container 1 from one end to an opposite end.
- the bioreactor 1a also has a drive device 2, which is arranged substantially outside the container 1.
- the stirring element 3 is coupled to the drive device 2.
- the stirring element 3 has a stirring 9, which is formed substantially rod-shaped.
- the agitator shaft 9 is arranged substantially completely in the interior (on the inside of the container I) of the container 1 and can either project from one end of the container 1 into the container 1 or the container 1 completely from a first end of the container 1 to a second End of the container 1, in particular from a container lid (not shown here) to a container bottom 1 'penetrate.
- the stirring shaft 9 is supported at two opposite ends of the container 1.
- the stirring shaft 9 is mounted on a drive-side support 6 and on an abutment 7 on the container bottom V.
- the drive-side bearing 6 is arranged directly adjacent to the drive device 2, while the counter-bearing 7 is arranged on the side of the container 1 opposite the drive device 2 or on the container bottom 1 '.
- the stirrer shaft 9 more Rownfort instruments 5 are formed, which move on rotation of the stirring shaft 9 about an axis of rotation of the stirring element 3 through the medium 8 and the medium 8 mix it.
- a stirring extension 5 can also be referred to essentially as a rotor blade.
- the container 1 or the bioreactor 1 a may alternatively also without agitator shaft 9 and / or without drive device 2 and / or without drive-side support 6 and / or without counter bearing 7 and / or scrubber 3, in particular without any element of the mixing device, which Mixing of the medium 8 can serve, be configured.
- the container 1 thus has on the container inner side I a container interior 22 which may be completely or partially filled with a medium 8.
- the container 1 at the time of irradiation with an electromagnetic radiation 14 of a medium 8 may be at least partially or completely filled.
- the medium 8 is stirred or mixed during the irradiation.
- all illuminant receiving pockets 11 may at least partially be filled with the respective illuminants 15.
- only part of the light-receiving pockets 11 may be at least partially filled with the respective light-emitting means 15.
- the lighting situation (lighting intensity or power, lighting position, emitted spectrum) can be adapted to the circumstances by adding, removing and / or replacing a lighting means 15.
- the light-emitting surface of the luminous means 15 can be varied in that a luminous means 15 only partially or completely protrudes or pushed into the bulb receiving pocket 11.
- a container interior 22 is at least partially filled with a medium 8, but no bulbs 15 is disposed in at least one bulbs receiving pocket 11, the bulb opening in the container wall 12a of the container shell 4 with a lid (not shown here ) are closed, so that the medium 8, for example, a liquid, the corresponding unfilled lamp-receiving pocket 11 is not inverted outwardly or on the outside A or presses.
- the lid or closure can substantially prevent leakage of a light-emitting receiving pocket 11 on the outer side A.
- the light-emitting receiving pockets 11, which are arranged on an inner side of a container waist and adapted to receive a respective light-emitting rod 15a, can in particular have a pocket length TL of about 0.1 m to about 2 m, preferably about 0.3 m to about 1, 5 m and more preferably about 0.5 to about 1 m.
- the diameter of the bulb receiving pocket 11 may generally be between about 5 cm and about 50 cm, preferably between about 8 cm and about 30 cm, and in particular between about 10 cm and about 20 cm.
- the ratio between the diameter and the pocket length TL can be approximately 1: 5000 to approximately 1:10, for example, in the case of illuminant receiving pockets 11 for receiving optical fibers 15b, especially about 1: 1000 to about 1:50, and preferably about 1: 300 to 1: 100.
- the ratio between the diameter and the pocket length TL can be, for example, in the case of light-emitting receiving pockets 11 for receiving light-emitting rods 15a from about 1: 500 to about 1: 1, in particular from about 1: 100 to 1:10 and preferably from about 1: 50 to 1:20.
- the pocket length TL, as well as the diameter can be selected essentially depending on the size of the reactor or of the container 1, in particular depending on the container width BB and the length and the diameter of the light-emitting rods 15a.
- the bulb receiving pockets 1 adapted to receive another light-emitting element or bulb 15 may have other pocket lengths TL.
- a light-receiving pocket 11 for receiving a light guide for example, a pocket length TL of about 1 m to about 10 m, in particular from about 1, 5 m to about 8 m and more preferably from about 2 m to about 7 m.
- the container width BB may in particular be about 0.3 m to about 3 m, preferably about 0.5 m to about 2 m, and particularly preferably about 0.8 m to about 1.5 m.
- the container interior 22 of a container 1 of a bioreactor 1a may, for example, assume values between about 15 ml and about 30,000 l, in particular between about 51 and about 8,000 l, and preferably between about 100 l and about 1000 l.
- FIG. 2a Another embodiment of a container 1 of a bioreactor 1a is shown in the exploded view of FIG. 2a.
- the container 1 of the bioreactor 1a comprises a container lid 1 "which can be removed or removed from the container 1 or from the upper edge O of the container 1.
- the container lid 1" comprises illuminant openings 12, in particular illuminant openings in the container lid 12b, on each of which a luminous means receiving pocket 11 is attached can or is appropriate.
- the illuminant receiving pockets 11 hang down substantially perpendicularly from the container lid 1 "into the container interior 22 in the direction of the container bottom 1 '.
- a light emitting means 15 on the left side L which is a light emitting rod 15a in this embodiment, is aligned relative to the container lid opening 12b, it is substantially not picked up by the light emitting means receiving pocket 11. It could be that the lighting means 15 is pushed into this position just in the pocket inner volume V of the light-emitting means receiving pocket 11 on the left side L, or removed therefrom.
- the light-emitting rod 15a along its longitudinal axis LA must be moved substantially perpendicular to the container lid 1 "through the bulb opening in the container lid 12b in the container interior 22 in the direction of the container bottom 1 '.
- a luminous means 15 on the right side R which is also a light-emitting rod 5a in this embodiment, is aligned relative to the luminous means opening in the container lid 2b and substantially completely received by the illuminant-receiving pocket 11.
- the longitudinal axis LA of the light-emitting rod 15a is arranged or aligned essentially parallel to the container casing 4 or container inner wall or container casing inner surface 4a on the container inner side I.
- a light-emitting rod 15a extends from the container lid 1 "toward the container bottom V, in particular to the container bottom 1 'within the pocket internal volume V of the illuminant-receiving pocket 11 located in the container interior 22.
- a light-emitting rod 15 a of the container lid 1 "and the receiving input AE of the bulb receiving pocket 11 in the direction of the container bottom 1 to the end E of the bulb receiving pocket 1 first
- the medium 8 can be switched on by the electromagnetic radiation 14 emitted by the light-emitting rod 15a Condition or be irradiated during operation.
- light emitting bars 15a or other bulbs 15 may be added, removed and / or replaced to adapt the lighting situation to, for example, a process.
- an irradiation intensity or output can be varied.
- a current or voltage or energy supply of the light-emitting rods 15a can be effected for example via a line 13, in particular via a mains cable.
- the mixing device comprising a drive device 2, a stirring element 3, rotor blades 5, a drive-side bearing 6 and an abutment 7 and a stirring shaft 9 are operated such that a medium 8 is mixed or mixed or stirred.
- a flow professional! of the medium 8 which results in that any molecule of the medium 8 undergoes, on average, a necessary irradiation of an uninterrupted irradiation time by the lighting means 15 in such a way that a photochemical reaction is triggered.
- the illuminant opening in the container lid 12b, as well as the illuminant receiving pockets 11 may for example be distributed radially symmetrically or not symmetrically along the lid edge circumference.
- the Flg. 2b is a view of various embodiments of the container lid 1 ", embodiments 1 to 4. According to embodiments 1 and 2, illuminant openings in the container lid 12b and the illuminant receiving pockets 11 are radially symmetrical about the center of the container lid 1" at substantially equal intervals. distributed substantially along the lid edge circumference. In Embodiment 1, all the illuminant openings in the container lid 12b are substantially circular, whereas in Embodiment 2, all the illuminant openings in the container ceiling! 12b: are substantially rectangular or designed.
- illuminant openings in the container lid 12b, as well as the illuminant receiving pockets 11 are not radially symmetrical about the center of the body at substantially equal intervals It may also be possible, for example, for a container lid 1 "to comprise a bulb opening 12b, which are of circular design and others which are rectangular in shape. Depending on which shape is predetermined by the lighting means 15 and which lighting situation is desired or required, any desired combinations of shapes and positional distribution of the lighting means openings in the container lid 12 b can be provided.
- FIG. 2 b furthermore, three rotor blades 5, as well as one central agitator shaft 9, can be seen.
- the container lid 1 is substantially transparent, or has an opening at the respective location, for example an addition of a substance into the medium 8 or into the container interior 22 could take place through such an opening.
- the embodiments of the bioreactors 1a or container 1 are preferably designed to be at least partially, in particular completely sterilized. At least the container interior 22, as well as elements of the bioreactor 1a, which are or are to be arranged in the container interior 22, are preferably designed to be at least partially, in particular completely sterilized.
- the bioreactors 1a may also be designed to form a closed system, as a rule. In other words, such bioreactors 1a can be omitted in most cases in all cases in particular the opening, especially when a medium 8 undergoes a process or one or a plurality of reactions. In particular, for purposes of sterilization of the container interior 22, a container 1, however, can be opened.
- the light-emitting receiving pockets 1, which are arranged on an inner side of a container lid and adapted to receive a light-emitting rod 15a in each case, can in particular have a pocket length TL of about 0.1 m to about 5 m, preferably about 0.3 m to about 3 m and more preferably about 0.5 to about 2 m.
- the pocket length TL can be selected essentially according to the size, in particular according to the container height BH of the reactor or of the container 1 and the length of the light-emitting rods 15a.
- the container height BH may in particular be about 0.5 m to about 5 m, preferably about 1 m to about 3 m, and particularly preferably about 1.5 m to about 2.5 m.
- FIG. 3 relates to a single use (SU) or disposable prey! 1 b. Since the single use (SU) or disposable bioreactor / disposable bag is designed to receive one or more lamps 15, this can also be referred to as single use (SU) or disposable photo-bioreactor.
- the disposable bag may be maintained or supported, for example, by means of a rack or scaffold (not shown).
- the disposable bag 1 b can also be arranged within a bioreactor, or part of a bioreactor.
- a light source 15d preferably in the form of a laser, which generates light or electromagnetic radiation 14, which is transported and emitted through a light guide 15b into the container interior 22, is provided in this embodiment ,
- the light guide 15b is guided by a bulb opening in the container wall 12a from the outside A to the container inside I.
- the optical fiber 15b is attached to a stirring shaft 9 or wound around a stirring shaft 9.
- the light source 15d or the laser is connected via a line 13, in particular a power cable to the network and receives the required energy for operation via the cable.
- the optical waveguide 15b is designed such that it can emit or emit a light 14 substantially over its entire length or at least over a portion of its length on its surface. This essentially depends on that a light is coupled into the light guide 15b in such a way that the Total reflection condition is not met or the angle at which the light 14 is reflected does not correspond to the total reflection angle. Thus, a fraction of the total guided light 14 is emitted at each reflection to the surrounding medium 8 and the container interior 22, respectively.
- the light guide 15b may be wound around a non-rotating part of a stirring shaft 9, for example around an outer rigid guide tube, or disposed thereon in any other form.
- the light guide 15b may be wound around the agitator shaft 9 for about 5 to about 500 times, preferably about 10 to about 300 times, and more preferably about 15 to about 100 times.
- a light guide 15b or a multiplicity of light guides 15b projects at least partially from the container casing 4 into the container interior volume 22 or protrude into it.
- the container interior 22 of a container 1 or a disposable bioreactor or a disposable bag 1b for example, values between about 15 ml and about 8000 I, in particular between about 80 I and about 5000 I, and preferably between about 100 I and about To accept 1000 l.
- a disposable bioreactor or a disposable prey! 1b to be used if a subsequent cleaning or sterilization is omitted, for example, for cost reasons. This may be the case when a particularly toxic medium 8 has been stored in the container interior 22 of a disposable bag, which can be disposed of more economically together with the disposable bag. Also, in a variety of ongoing processes in a variety of bioreactors, it may generally be more cost effective to use disposable bioreactors / disposable bags than reusable bioreactors. Often, the purchase of a reusable bioreactor 1a, for example of steel, and cleaning or sterilization of such reusable bioreactors 1a proves to be inefficient in terms of cost. In such cases, disposable bioreactors such as disposable bags 1b are preferred.
- a preferred embodiment of a container 1 of a disposable bioreactor for disposable use may comprise a container envelope 4 or other elements, which is essentially or at least partly formed from a so-called "soft plastic” or from a particularly flexible plastic, in particular a flexible PVC and / or polyolefin, in particular polyethylene
- Such elements and / or sections may in part be formed from a so-called "hard plastic” or from a more rigid or dimensionally stable plastic, in particular from a (fusible) thermoplastic or from a (non-fusible) thermoset, for example a synthetic resin his.
- a container access or a port 16, and hoses 18 and portions of a container bottom V could be formed of such a more dimensionally stable plastic.
- the plastic is substantially or at least partially sterilizable e.g. using beta or gamma radiation.
- the material used can be sterilized by means of thermal sterilization, by steam sterilization, by hot air sterilization, by means of chemical and / or physical sterilization (for example beta or gamma irradiation) for producing a container 1 or for producing a bioreactor 1a.
- FIG. 3 further comprises a mixing device comprising a stirring element 3 with a stirring shaft 9 and rotor blades 5, a drive device 2 and a drive-side mounting 6 for mixing or stirring the medium 8 located in the container interior 22.
- a mixing device comprising a stirring element 3 with a stirring shaft 9 and rotor blades 5, a drive device 2 and a drive-side mounting 6 for mixing or stirring the medium 8 located in the container interior 22.
- elements of the Mischvom 'rect are at least partially formed of a metal and / or a stable plastic.
- an element may also include a metal core coated with a substantially inert or non-reactive plastic, such as Teflon.
- the disposable container / disposable bag 1 b or the disposable bioreactor is designed essentially for disposable use, wherein all elements are used only once.
- elements, such as a mixing device for reusable use can be designed.
- all elements and surfaces located at least within the container interior 22 can be sterilized.
- the container casing 4 of the disposable bag 1 b is preferably transparent to electromagnetic radiation of at least one frequency range, in particular for at least part of the visible light.
- a medium 8 located within the container interior 22 can be at least partly also irradiated, for example, from the outside A by means of an artificial light source 5d or light source, preferably by means of a lamp and / or by means of a natural light source 15d, such as the sun.
- a mixing device does not necessarily have to be part of a (one-way) bioreactor.
- a light source 15 and / or a light source 15d may also be mounted in or in the disposable photo-bioreactor in another alternative manner.
- a disposable bioreactor / disposable bag 1 b can in particular comprise light-emitting means receiving pockets 11.
- bulb receiving pockets 11 are similar to the previous embodiments, for example, Fig. 1 and Fig. 2 configured.
- Bulb-receiving pockets 11 are preferably designed substantially to protrude on the container inside I of the disposable bioreactor / disposable bag and from the outside A a light source 15, in particular a light-emitting rod 15 a record, so that the light-emitting Rod 15a, which is isolated only by the pocket wall 11a of the medium 8, an electromagnetic radiation 14 to the container inside I or to the medium 8 in the container interior 22 can emit.
- Such light-receiving pockets 11 are preferably made of a same material as a majority of the container shell 4 and in particular transparent.
- sections of the container casing inner surface of a disposable bag 1b can also be provided, in particular, flatly with a light source 15, for example with a light-emitting foil or a light-emitting diode, or with light-emitting diodes (LEDs) or other light sources , Additionally or alternatively, in the container interior 22 also light be provided emitting particles.
- a light source 15 for example with a light-emitting foil or a light-emitting diode, or with light-emitting diodes (LEDs) or other light sources
- LEDs light-emitting diodes
- the agitation 9 of the disposable bioreactor / disposable bag 1 b may include a cavity, as well as a substantially transparent wall, so that a light source, such as a light-emitting rod 15 a can be accommodated in the cavity and through the transparent wall of the Rjoweile 9 a light 14 to the medium 8 and the container interior 22 can deliver.
- a light source such as a light-emitting rod 15 a
- Hoses 18 and sensors 19 are also disposed on the disposable bag 1 b of the present embodiment.
- the sensors 19 are each connected to lines 13.
- Such lines 13 may be, for example, power and / or data cable.
- a conduit 13 may also be a hose. This is not explicitly defined in this embodiment.
- Substances and / or a medium 8 can be conducted into the container interior 22 via the tubes 18 and / or be discharged from the container interior 22 onto an outside A or removed.
- Container inlets 16 and / or ports may be configured to permit a hose to be respectively disposed thereon.
- container accesses 16 and / or ports may be designed to receive a lighting means.
- a port 16 may be a sealed access for a light emitting rod 15a (not shown).
- container entrances 16 and / or ports and / or container doors can be designed, for example, such that a sample of the medium 8 can be taken by hand, for example.
- a disposable bioreactor / disposable bag 1 b include a pressure relief valve (not shown here), which can, for example, a gas from a certain pressure threshold release or release or escape.
- the Flg. 4 is an embodiment of a container 1 of a bioreactor 1 a, in which a plurality of light sources 15, for example in the form of light guides 15 b, which can emit electromagnetic radiation during operation, for irradiating a medium 8 located in the container interior 22, are provided.
- the plurality of luminous means 15 comprises about 3 to about 1000, preferably about 10 to about 500, and more preferably about 20 to about 300 optical fibers 15b and / or light-emitting rods 15a.
- the bioreactor 1a or the container 1 may themselves comprise the light guides 15b, or alternatively the light guides 15b may be arranged on the container 1 of the bioreactor 1a.
- the light guides 15b may be picked up by a single light-emitting receiving pocket 11 or each individual light guide 15b by a respective light-emitting receiving pocket 11 and insulated from the medium 8 by the pocket wall 11a .
- the light guides 15b may also be in direct contact with the medium 8, in particular if the light guides 15b are designed to be sterilizable.
- the illuminants shown may alternatively or additionally also be light-emitting rods 15a.
- the illuminants 15 are attached to the container lid 1 "or to the container cover 12.
- the portions of the illuminants 15 which are arranged substantially on the container inside I are provided with elements
- a light source 15d may in turn be connected by means of a line 13, for example a power cable, through which the light source 15d is supplied with energy, in order to allow particularly large-area or efficient irradiation
- the illuminants extend substantially from the container lid 1 "substantially to or almost to the container bottom 1 '.
- the light guides 15b and / or light-emitting rods 15a may preferably extend over a length of between about 0.1 and 5 m, in particular between about 0.3 m and about 3 m, and more preferably between extend about 1 m and 1, 8 m.
- a light guide 15b may have a length of about 0.1 m to about 50 m, in particular about 2 m about 30 m and preferably from about 3 m to about 15 m.
- the container casing 4 of the container 1 of FIG. 4 may be substantially non-transparent and preferably formed of a metal, for example steel.
- the container shell 4 of the embodiment shown may be at least partially or completely transparent, in particular made of a glass and / or a transparent plastic.
- the container shell 4 of the container 1 can also comprise a transparent window, through which an electromagnetic radiation can pass from the outside A to the inside of the container I, for example emitted by an external light source.
- the container 1 of the bioreactor 1a according to FIG. 4 is supported by a framework 21 or stored and / or suspended and / or held.
- the framework 21 has rollers 24, by means of which the bioreactor 1 a can be pushed and / or pulled to a destination.
- the container 1 of the bioreactor 1a further comprises container ports 16 or ports, through which the medium 8 is accessible for the addition or removal of a substance.
- drains 23 which can also be used as feeds, a medium 8 can be at least partially drained from a container interior 22 or a container interior 22 can be filled with a medium 8 at least partially.
- a hose can be attached to a drain 23.
- a container 1 In the embodiment of a container 1 according to the exploded view of Figure 5, there are a plurality of different light sources 15.
- the container 1 can it is in particular a disposable container.
- the container 1 may be formed of a substantially stable or rigid or solid material, for example, the container 1 at least partially made of a metal, in particular steel and / or at least partially made of a solid plastic, in particular of a transparent plastic and / or be formed of glass.
- a disposable container 1 may be a disposable bag.
- an external light source 15f is initially indicated.
- the external illuminant 15f may be a lamp or other light source 15d disposed on the outside A such that a light 14 emitted by the lamp may propagate through a substantially transparent portion of the container shell 4 into the container interior.
- a medium 8 can also be irradiated from the outside.
- the container 1 further comprises a container casing 4, as well as a container lid 1 ", which can be arranged on the upper edge O of the container 1.
- the container interior 22 or the container inner side I can be permanent or for a longer time from the outside A be isolated or over a period of time, for example, by opening the container lid 1 "open or be open.
- the container cover 4 and the container lid 1 each comprise illuminant openings 12, namely two illuminant openings in the container wall 12a, and a luminous means opening in the container lid 12b
- the container interior 22 and the outside A are separated by a sealed connection between the illuminant opening 12 and the illuminant receiving pockets 11, so that no medium 8 which is located in the container interior 22 can unintentionally escape to the outside
- the bulb receiving pockets 11 each include a dense pocket wall 11 a.
- a light-emitting means 15, in particular a light-emitting rod 15 a, can be pushed or moved and / or arranged or stored in a light-receiving pocket 11 or in a pocket inner volume V of a light-receiving pocket 11 become.
- two light-emitting rods 15a are each on the right side R still on the outside A. Both light-emitting rods 15a can by moving or in the direction of the container shell 4, which in the figure is drawn, substantially horizontally through the container wall openings 12a and the openings in the container casing 4 and the receiving inputs AE of the bulbs-on receiving pockets 1 1 in the respective pocket-internal volume V moves or pushed.
- FIG. 5 there is also a light-emitting rod 15a above the container lid 1 '' on the outside A.
- the light-emitting rod 15a can be moved by a movement in the direction of the container lid 1 '' which is in the Figure is drawn through the container lid opening 12b and the openings in the container lid 1 "and the receiving input AE of the light-receiving pocket 11 are moved or pushed into the pocket inner volume V.
- the container 1 may comprise fastening means 26, for example a thread and / or a pressure connection and / or a clamp.
- a light-emitting rod 15a or other illuminant 15 may then be fixed to the container 1 by means of a mating fastener, for example by means of a screw and / or a groove and / or a hook.
- a lighting means 15 can then emit electromagnetic radiation.
- the container lid 1 also includes Bulb opening in the Be ZellerdeckeS 12 b, through which optical fibers 15 b can be guided from the outside into the container interior 22.
- the light guides 15b may be isolated from the medium 8 by one or more light receiving pockets 11, or may be in direct contact with the medium 8.
- the container shell 4 comprises a container shell inner surface 4a, which is an inner surface of the container shell 4 facing the container interior.
- the container shell 4 comprises a container envelopefigurationfikiee 4 b, which is an outer side A facing outer surface of the container shell 4.
- the container door 16 is an access to the container interior 22 or to the container inside I from the outside A.
- the container door 16 comprises a door wall 4c, whose inner surface in the closed state is preferably flush with the container envelope inner surface 4a concludes.
- the outer surface of the door wall 4c in the closed state preferably ends flush with the container outer surface 4b.
- the inside of the door wall 4c comprises a lighting means 15, which may be, for example, a light-emitting film 15c.
- the light-emitting film 15c can emit or emit an electromagnetic radiation 14 to the container interior 22 and a medium 8 located therein, in particular in the closed state.
- a container casing 4 comprises a container casing outer surface 4b, as well as a structured, in particular corrugated container casing inner surface 4a or a container inner wall structuring 17, which essentially faces the container inner side I.
- the container Inner wall structuring 17 may, as shown in the present embodiment, comprise projections and indentations.
- the structured or corrugated or spiral formation of the container envelope inner surface 4 a can provide a larger surface, on which illuminants 15 or light sources can be arranged, and from which a light 14 can be emitted into the container interior 22.
- light-emitting rods 15a On the container shell inner surface 4a, light-emitting rods 15a, optical fibers 15b and / or light-emitting films 15c, light sources 15d, in particular light-emitting diodes 15e may be arranged so that these can emit an electromagnetic radiation 14 to the container interior volume 22 during operation.
- the container inner wall structuring 17 produces an area 27 which is larger than or compared to an unstructured container envelope inner surface 4a and on which substantially more illuminants 15 can be arranged than on an unstructured container envelope inner surface 4a.
- the container inner wall structuring 17 can also be structured in such a container 4a covering inner surface such that a preferred flow profile of a medium 8 can be produced 'in particular, when a container 1 has a mixing apparatus which can mix or stir the medium. 8
- FIG. 7 shows a further embodiment of a container 1 of a bioreactor 1 a comprising different light sources 15 and / or light sources.
- the container 1 of the bioreactor 1a comprises a container casing 4 with a container casing inner surface 4a and a container casing outer surface 4b.
- the container shell inner surface 4a may comprise regions or sections on which light-emitting elements or illuminants may be arranged.
- Such light-emitting container Inner wall sections are identified for example in the present figure by the reference numerals a and b.
- a light-emitting film 15c may be disposed on a light-emitting container inner wall portion a.
- light-emitting diodes 15e may be disposed on another light-emitting container inner wall portion b.
- Such lamps 15 can be supplied via a battery and / or a mains supply by means of one or more power cables with the energy required for operation (not shown here).
- the lighting means 15 on the container casing inner surface 4a can be isolated from the medium 8 by means of a film, for example.
- the bulbs 15 on the container shell inner surface 4a may also be reversibly attachable and removable.
- the container shell inner surface 4a may comprise plug-in connections to which lighting means 15, for example light-emitting diodes 15e, can be attached.
- the present embodiment of a container 1 of a bioreactor 1 a also includes a mixing device with rotor blades 5 or Rownfort ordersn. On the surfaces of the rotor blades 5, light-emitting elements or lamps 15 may preferably be arranged.
- Light-emitting rotor blade sections are indicated in particular by the reference symbols c, d and e. It is indicated on a preferred light-emitting rotor blade section c that a light-emitting film 15c is arranged thereon.
- Another preferred light-emitting rotor blade section d indicates that a multiplicity of light-emitting diodes 15e are arranged thereon.
- An ' other preferred light-emitting rotor blade section e is indicated that a plurality of optical fibers 15b is arranged parallel to each other.
- One or more rotor blades may also be formed only in accordance with the rotor blade section c or d or e.
- a container 1 of a bioreactor 1 a comprises a stirring shaft 9, which extends substantially from the container ceiling to the container bottom V.
- the stirring shaft 9 comprises a stirring shaft cavity 9a, as well as an at least partially transparent stirring shaft wall 9b.
- a light-emitting rod 15 a and / or a light guide 15 b and / or a (substantially rod-shaped) light source 15 d can be picked up by the stirring wave cavity 9 a, so that the light-emitting rod
- 15a can emit an electromagnetic radiation 14 at the, preferably at least partially filled with a medium 8 container interior 22 during operation.
- the container 1 of the bioreactor 1 a comprises a container access 16 with an access tube 16 b and a valve 6 a arranged substantially on the right side R on the upper container cover. Through the container access 16 substances from the outside A, such as gases and / or liquids in the container interior 22 can be passed.
- the container 1 of the bioreactor 1a also comprises substantially on the left side L on the container cover an access 16 with a container lid 1 "or container door or a container flap, softer or which can be opened and closed such an opened container lid 1 "can be supplied to the medium 8 on the inside of the container I, for example, a granulate or other solid from the outside A.
- the container 1 of the bioreactor 1 a also comprises a plurality of other container accesses 16 on the container envelope 4, by means of which substances can be supplied to or removed from the container internal volume 22. -,
- Fig. 8a is a frontal view of a steel-lined bioreactor 1a comprising a disposable bag 1b corresponding to or at least similar to the embodiment of the disposable bag 1a of Fig. 3 in its internal volume.
- Fig. 8b is a frontal view of a partially opened bioreactor 1a with a steel shell and opened container door 16, which includes a disposable bag 1b visible in its interior volume according to the embodiment of Fig. 8a.
- Fig. 8c is the frontal view of a disposable bag 1a according to or at least similar to the embodiment of the disposable bag 1a of Flg. 3, which can be absorbed by the bioreactor 1 a with Stahlhüile according to the embodiment of Figure 8a and b.
- FIG. 9a is a front view showing a container 1 of a bioreactor according to an embodiment.
- light-emitting rods 15a are embedded in the peripheral wall of the container shell 4 'and in the ceiling of the container shell 4.
- the light-emitting rods 15a are arranged by means of illuminant openings 12a in the container wall or in the container casing 4 on the inside of the container 1, and in particular within lamp receiving pockets 11.
- the light-emitting rods 15 a are also arranged by means of illuminant openings 12 b in the container ceiling on the inside of the container 1, and in particular within lamp receiving pockets 11.
- the container 1 comprises at least one luminescent medium opening 2b on a section of a container ceiling, and at least one illuminant opening 12a on a section of a container wall or a container envelope 4.
- an illuminant opening 12a, 12b Receiving pocket 11 is arranged.
- the light-emitting rods 15a are not arranged inside light-emitting receiving pockets 11, but are in direct contact with the interior or the medium in the interior. This would be the case in particular if the light-emitting rods 15a are permanently arranged on the container 1 or are not removed from the container when it is filled with a medium.
- Fig. 9b is a view of three light-emitting rods 15a according to an embodiment.
- the light-emitting rods 15a each include a handle G for handling.
- the grip G may also serve to prevent the light-emitting rod 15a from being touched directly at a possibly hot and / or sensitive location.
- the handle G may also serve to remove the light-emitting rod 15a from the container 1 when it is placed on and / or in the container 1.
- the light-emitting rod 15a is connected by means of a conduit 13 or by means of powered by a cable.
- FIG. 10a is a front view showing a container 1 of a bioreactor according to an embodiment.
- the rods and / or the light-emitting rods 15a with the light-emitting fibers / optical fibers 15b are arranged on the inside of the container 1 by means of illuminant openings 12a in the container wall or in the container casing 4. They may be in direct contact with the inside and / or the medium on the inside or may be located inside bulb receiving pockets 11.
- the light-emitting rods 15a are also arranged by illuminant openings 2b in the container ceiling on the inside of the container 1.
- a lighting means 15 may include a light-emitting rod 15a on which light-emitting fibers 15b are disposed.
- a lighting means 15 may generally comprise a bar on which light-emitting fibers 15b are arranged.
- a lighting means 15 may comprise a hollow transparent bar having an internal space through which light-emitting fibers 15b are led, which emerge from the interior at different openings of the hollow transparent bar.
- This embodiment has the advantage that the surface of the luminous means 15 is designed to be particularly large. In other words, a particularly large area is generated which can emit light, in particular to a medium surrounding the illuminant 15.
- the high-end rod may also be regarded as the light-emitting rod 15a although it does not emit light but the light-emitting fibers 15b itself.
- the light-emitting fibers 15b are made flexible so that they can partially move with a moving medium.
- a plurality of light-emitting fibers 15b are also arranged like a bristle.
- an attachment device 29 for a plurality of optical fibers or light-emitting fibers 15b is arranged on the agitator shaft.
- a hollow transparent stirrer shaft 9 within the cavity of which a luminous means 15, for example a light-emitting rod 15a and / or a light-emitting fiber 15b, is arranged.
- the light-emitting fibers 15b may be in direct physical contact with a medium on the inside of the container 1 or may be disposed within one or more light-receiving pockets 11.
- FIG. 10b is a perspective view of a mounting device 29 for a plurality of light emitting fibers 15b according to an embodiment.
- the attachment device 29 comprises a ring 29 ', for example a metal and / or plastic ring.
- the ring is made transparent.
- circular light-emitting fibers 15b are arranged, which are away from the ring 29'.
- the light-emitting fibers 15b are arranged circularly side by side in a row of a plurality of light-emitting fibers 15b. In the illustrated embodiment, twelve rows of three superimposed fibers 15b are arranged circularly. Alternatively, another number can be selected.
- Fig. 10c is a perspective view of three rods and / or light-emitting rods 15a with light-emitting fibers / optical fibers 15b.
- the bars each comprise a handle G and a cable 13 for power supply.
- illuminants 15 can be arranged, in particular, flat on the inside of the wall of the biofilter 1a or steel tanks, so that illumination of the medium 8 in the disposable bag 1b can take place through the container casing 4.
- a light source 15, in particular a fiber or an optical waveguide 15b can be arranged, in particular wound around the agitator shaft, at least partially or in sections.
- the bioreactor 1 a comprises a computing unit 28 which is designed to control and / or regulate, for example, a temperature, a pump flow, a pressure and / or an illuminance by the lighting means 15.
- Containers 1, 1b, mixing systems and pellet tanks essentially serve to receive, store and mix biological media 8, e.g. Fluids and / or solids and / or gases.
- biological media may be stored in containers 1, 1b, e.g. Be bags 1 b, in particular provided in plastic bags, which may comprise a volume of several hundred liters.
- the biological media 8 can preferably be introduced into the bioreactor 1 a within such a bag 1 b, in which they can be stored, tempered and / or mixed and irradiated. In such a container 1 of the (photo) bioreactor different photoreactive processes or reactions within the biological medium 8 can be triggered.
- a container 1, 1 b may be part of a bioreactor 1 a and / or a fermenter.
- a container may also be part of a food tank or food keg or a silo or a store.
- a container 1, 1 b may also be part of a laboratory device, in particular a chemical laboratory device.
- a container may be column for column chromatography.
- a light-emitting receiving pocket 11 may, for example, preferably be made of a soft plastic as a flexible and / or stretchable and / or foldable and / or movable pocket (in the sense of a bag or a pocket).
- a bulb receiving pocket 11 may also be a hollow and substantially transparent tube of a glass and / or a hard plastic or resin, which may be designed for multiple use and sterilizable.
- medium 8 in particular liquids, gases, suspensions, dispersions, buffers and / or cell culture broths are considered as medium 8 or medium 8.
- Media 8 may also include solids such as powders, granules, pressed pellets, particles, granules, and mixtures thereof.
- a medium 8 can accordingly different components with same or different state of aggregation, for example comprise an emulsion or a dispersion.
- a photoreactive or photosensitive medium may in particular comprise biochemical materials, in particular biological materials.
- a medium may include plants, in particular algae and microalgae and / or bacteria, in particular purple bacteria and / or cyanobacteria, fungi, plant and / or animal cells, for example moss cells, eukaryotes and / or prokaryotes, and corresponding mixtures thereof. It is even possible for whole plants, such as duckweed and / or tissue stabilizers, to be contained in the medium, for example in a suspension.
- the irradiation can trigger a biochemical process, in particular photosynthesis in cells.
- the media are substances which undergo biological processes, in particular heterotrophic, phototrophic and / or mixotrophic processes.
- photoreactive or photosensitive or photosensitive substance generally includes molecules and atoms, as well as larger building blocks of molecules and / or atoms, for example macromolecules or quantum dots, which respond to an irradiation with light or electromagnetic waves by means of a chemical and / or govern biological and / or biochemical and / or physical reaction.
- media for example the aforementioned, comprise proteins that undergo photochemical processes by the irradiation of light.
- plants, algae and cyanobacteria contain so-called complexes, ie proteins in lipid / cell membranes, which are responsible for the conversion of photosynthesis.
- photoreactive or photosensitive or photosensitive substances can be, for example, photosensitive proteins.
- azobenzene, hydrogen peroxide, fluorescent and / or phosphorescent substances, quantum dots, graphenes or other substances can also be involved in photochemical reactions.
- photoreactive material, photosensitive substance and Fichtiffer substance have substantially the same meaning and mine, for example, previously mentioned substances that can react by means of light or light exposure.
- photoreactive or photosensitive or light-sensitive substances are meant, each undergoing a photochemical particular photo-biochemical reaction. It may be meant such photoreactive or photosensitive or photosensitive substances which operate photochemical processes, such as photosynthesis and reversibly undergo various stages or states. However, it may also be meant those substances which are educts which convert to products by means of light irradiation.
- the photochemical reaction can be carried out in stages of various photochemical reactions or in stages of different reaction at least comprising a photochemical reaction. Also included are physical and / or biological reactions or phenomena from the term "reaction.” For example, electromagnetic (heat) radiation may cause the pressure and / or within a reservoir interior to increase, which in turn affect the photochemical reaction can.
- a photochemical reaction is typically understood as meaning those chemical reactions which are initiated, initiated or driven under the action or absorption or irradiation of light or electromagnetic radiation.
- an absorption of light or eiektromagnetêt radiation by a molecule or atom, which participates in the photochemical reaction is usually necessary. This means that the wavelength or frequency of the light used must be matched to the absorption behavior of the molecule.
- This essentially relates to an immediate or direct photochemical reaction.
- photochemical reactions or photoreactions in which a so-called photosensitizer is first excited by light and then this energy is transferred to molecules that participate in a chemical reaction, for example in an endothermic reaction. In this way, a chemical reaction can be triggered indirectly.
- a process is typically understood as meaning those chemical reactions which are initiated, initiated or driven under the action or absorption or irradiation of light or electromagnetic radiation.
- a chemical process can comprise one or more reactions, in particular photochemical reaction. If a medium 8 undergoes a process, then it can be said that the medium 8 is processed, that is to say that it is converted from an initial state by one or more reactions into a final state.
- the medium 8 may initially comprise or be one or more educts, and at the end of the process, the medium 8 may comprise or be one or more products.
- such a photochemical reaction may include one or more of: molecular breaks, photoisomerizations, electrocyclic reactions, rearrangements, light-induced chain reactions, photo-Fries shifts, isomerizations, for example, cis-trans isomerizations, biological photoreactions, and photophysical Processes, in particular chlorination, electrocyclic reactions, nitrosylation, oxychlorination, oxidation, catalyzed oxidation, sensitized oxidation, cationic polymerization, radical polymerization, sulfochlorination, sulfoxidation. Often in such reactions electronic states changes in molecules and atoms by the absorption of light or photons, or electromagnetic radiation ⁇ caused or triggered.
- Photosynthesis especially the anoxygenic photosynthesis or the oxygenic photosynthesis of biological substances.
- Photosynthesis involves special proteins which can be present, for example, in plant or bacterial substances or can be obtained from plant or bacterial sources.
- a medium could include one or more species of algae that absorb light to drive photosynthesis.
- visible wavelength range refers to the wavelengths of light that are substantially visible to a human, in particular between about 380 nm to about 780 nm.
- visible wavelength range refers to the wavelengths of light that are essential to a human are invisible, for example, wavelengths shorter than about 380 nm or longer than about 780 nm.
- the term light used here is not limited to the visible spectral range, but rather relates to electromagnetic radiation in general.
- a "transparent wall” may be transmissive or transparent to at least a portion of the light of those wavelengths or frequency ranges.
- transparent wall is often limited to a light transmission of light of an at least partially visible spectrum. By contrast, a "transparent wall” can be transparent to visible and / or invisible light.
- a light source 15 may be, for example, a light source 15d.
- a light source 15 may also be an optical fiber 15b or an optical fiber, which merely transports or transports the light emitted by a light source 15d to a specific location where it is used, for example a medium 8 irradiate.
- the luminous means 15 to a light-generating source 15d or 15d act a light source or may alternatively, it or a light-transporting and particularly to a light-emitting means such as an optical fiber a light guide 15b act.
- light-emitting means 15 and light source 15d used here differ only in that a term of the light-emitting means 15 used in this description represents a generic term which, however, encompasses the term light source 5d.
- the light-emitting means 15 is characterized essentially by the fact that it emits light, in particular in the direct environment of the medium 8, but does not necessarily generate the light itself.
- lighting means 15 and light sources 15d are also referred to as light-emitting elements.
- Light sources 15d in particular light sources for the so-called “photosynthetic active radiation", which are used in photochemistry, in particular in photo-biochemistry and / or for photosynthesis, can generally be subdivided into continuous and discontinuous radiators
- light sources 15d are used which have an output of about 0.1 W to about 10000 W, in particular from about 5 W to about 6000 W and preferably from about 1500 W to about 5000 W respectively.
- Light sources 15d are also used which have a power of about 0.1 W / m 2 to about 10000 W / m 2 , in particular from about 5 W / m 2 to about 6000 W / m 2 and preferably about 1500 W / m 2 to about 5000 watts / m 2 have.
- Sunlight may have a power of about 500 to about 2000 watts / m 2 .
- the illumination of a bioreactor, in particular for cultivation in the high cell density range should have the same or preferably higher performance.
- Continuous light sources 15d can emit light which may have a wide wavelength range. Often black emitters are used. So-called black spots can be for example the sun or light bulbs.
- the frequency spectra of black emitters are characterized by a very wide or broad spectral distribution. The spectral distribution can range from the infrared, in particular the far-infrared range (heat radiation) over the visible frequency spectrum to the (near) UV range.
- the UV components of such black emitters can be low, which is why other light sources are often used for photosynthesis or - chemistry, which also cover a frequency range that at least partially covers or includes a UV range.
- Suitable continuous radiators in the UV range are, for example, gas discharge lamps based on hydrogen / deuterium or noble gases.
- a luminous flux may have a value of from about 10 lumens to about 10,000 lumens, more preferably from about 100 lumens to about 7,000 lumens, and preferably from about 500 lumens to about 6,000 lumens.
- the unit of this sum of PAR's light sum is mol / (sm 2 ). For example, a sum of light of about 40 to about 200 pmo / m 2 s can be emitted.
- the illumination can be done for example by means of a pulsed illumination, for example, stroboscopic. This results in a “flashing light effect” or a flashlight effect.
- the “flashing light effect” ensures that it is improved Photosynthesis in the cell occurs when the lines are exposed to short bursts of light rather than steady light.
- the “flashing light effect” or the flashlight effect can be achieved by suitable mixing in the photobioreactor, because light has only a small penetration into the culture and by the appropriate illumination duration, which succeeds by appropriate mixing (including with the help of Baffles or illuminated Baffles in SU bioreactor to the standard drive), the cells can circulate between healthy and dark zones in the bioreactor and thus grow faster due to the "flashing light effect".
- a disposable container with stirring device can be equipped with light-emitting surfaces in such a way that the said "flashing light effect" or the flashlight effect can be achieved by suitable mixing and thus suitable illumination times of the cells and / or bacteria and / or algae All of these forms for producing light-emitting surfaces can be combined in this respect, in particular in a disposable container / bioreactor.
- a light source 15d may generally include a laser, a diode, a globar, a Nernst lamp, an arc lamp, an incandescent lamp, a phosphor, a light emitting diode 15e (LED), and / or another light emitting means. Furthermore, sunlight can also be captured and / or bundled and directed into the container interior. Thus, the sun can serve as a light source.
- Light sources 15d can preferably emit electromagnetic radiation which lies at least partially in a frequency range which is visible to humans. Additionally or alternatively, light sources 15d can also emit light of a wavelength or frequency which lies at least partially or completely outside the frequency range visible to humans. For example, light sources 15d can emit a light which has a frequency in the UV range and / or in the infrared range. In particular, light sources 15d can emit light of a wide frequency spectrum. Alternatively, a light source 15d, such as a lens, may also emit light of very narrow band with respect to frequency.
- a bioreactor according to one of the aspects mentioned can furthermore comprise an active and / or passive tempering system, which can, for example, dissipate and / or supply heat. This can serve, in particular, to prevent overheating of living organisms in the medium is prevented by strong irradiation.
- an active and / or passive tempering system which can, for example, dissipate and / or supply heat. This can serve, in particular, to prevent overheating of living organisms in the medium is prevented by strong irradiation.
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Abstract
Description
Claims
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Application Number | Priority Date | Filing Date | Title |
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DE102017008769.5A DE102017008769B4 (de) | 2017-09-19 | 2017-09-19 | Beleuchtung für einen Einweg-Photo-Bioreaktor |
PCT/EP2018/074004 WO2019057508A1 (de) | 2017-09-19 | 2018-09-06 | Beleuchtung für einen photobioreaktor |
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EP3684904A1 true EP3684904A1 (de) | 2020-07-29 |
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EP18769120.9A Pending EP3684904A1 (de) | 2017-09-19 | 2018-09-06 | Beleuchtung für einen photobioreaktor |
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WO (1) | WO2019057508A1 (de) |
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DE102019110989A1 (de) * | 2019-04-29 | 2020-10-29 | RHENAC GreenTec AG | Licht- und Temperaturvorrichtung zur Optimierung der Produktion von Biomasse in Bioreaktoren |
US20210355419A1 (en) * | 2020-05-13 | 2021-11-18 | Sophie's BioNutrients Pte. Ltd. | Bioreactor system for cultivating microalgae |
DE102020114809A1 (de) * | 2020-06-04 | 2021-12-09 | EKATO Rühr- und Mischtechnik GmbH | Rührvorrichtung und Verfahren zum Betrieb einer Rührvorrichtung |
CN116761784A (zh) * | 2021-01-28 | 2023-09-15 | 昕诺飞控股有限公司 | 光反应器组件 |
CN113150951A (zh) * | 2021-05-13 | 2021-07-23 | 云南爱尔发生物技术股份有限公司 | 一种在光伏大棚中培育微藻的方法 |
CN113583809B (zh) * | 2021-08-26 | 2022-06-28 | 德州六顺电气自动化设备有限公司 | 一种基于微藻闪光效应的反应器补光器及其补光方法 |
DE102021124016A1 (de) | 2021-09-16 | 2023-03-16 | Ferdinand Bierbrauer | Bioreaktor und Verfahren zum Betreiben eines Bioreaktors |
DE102021126012A1 (de) * | 2021-10-07 | 2023-04-13 | Lightpat Gmbh | Bioreaktor |
EP4317396A1 (de) * | 2022-08-01 | 2024-02-07 | Sartorius Stedim Systems GmbH | Single-use baghalter zur verwendung mit platinen-einbauten |
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-
2017
- 2017-09-19 DE DE102017008769.5A patent/DE102017008769B4/de active Active
-
2018
- 2018-09-06 WO PCT/EP2018/074004 patent/WO2019057508A1/de unknown
- 2018-09-06 EP EP18769120.9A patent/EP3684904A1/de active Pending
- 2018-09-06 US US16/648,656 patent/US20200283710A1/en active Pending
Also Published As
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DE102017008769B4 (de) | 2022-03-10 |
US20200283710A1 (en) | 2020-09-10 |
WO2019057508A1 (de) | 2019-03-28 |
DE102017008769A1 (de) | 2019-03-21 |
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