EP3606330A1 - Substrate mixture for fungal material, fungal culture and use thereof - Google Patents
Substrate mixture for fungal material, fungal culture and use thereofInfo
- Publication number
- EP3606330A1 EP3606330A1 EP19703997.7A EP19703997A EP3606330A1 EP 3606330 A1 EP3606330 A1 EP 3606330A1 EP 19703997 A EP19703997 A EP 19703997A EP 3606330 A1 EP3606330 A1 EP 3606330A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- fungal
- substrate mixture
- nanocellulose
- culture
- cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
Definitions
- the invention relates to a substrate mixture for fungal material, a fungal culture, the use of the substrate mixture and the fungal culture and a method for the treatment of cellulosic, hemicellulose and / or lignin-based materials, soil and culture soil, agricultural and forestry waste, from
- Plants and wood, for bioaugmentation, for bioremediation or for biological control of cholera Plants and wood, for bioaugmentation, for bioremediation or for biological control of cholera.
- the vegetative, long-lived body of a fungus is an extensive network of microscopic filaments (known as mycelium) that completely penetrate the soil, tree trunks, or other substrates in which the organism grows.
- mycelium microscopic filaments
- Saprophytic fungi decomposers of wood and organic matter
- saprophytic fungi form a symbiotic, mutually beneficial relationship with a number of agricultural crops. For example, it is known that corn in the presence of straw bales inoculated with Stropharia rugosoannulata provides greater yields compared to unvaccinated straw bales.
- the no-till method of agriculture also benefits from the growth of
- Basidiomycetes enclosing fungi that break down plant stoppers into nutrients.
- Parasitic fungi have their own role in a healthy ecosystem, although they can become overly destructive in unhealthy systems.
- a other broad class of decomposers are the more primitive, non-fruiting fungi imperfecti, which also include molds and yeasts.
- fungi Without the presence of fungi, few organisms can effectively break down the complex biopolymers cellulose and lignin, the two major components of woody plants.
- Cellulose and especially lignin are generally resistant to microbial attack and degradation.
- the fungi, especially white rot fungi that can degrade lignin, and brown rot fungi, the main decomposers of cellulose produce a complex series of enzymes that completely oxidize the polymer structures to water and carbon dioxide. Both liquid substrate and solid substrate cultures of white rot fungi were
- PCP Pentachlorophenol
- TNT trinitrotoluene
- dioxin dioxin
- nitrogenous compounds such as ammonium nitrate, urea, purines and putrescine, as well as agricultural waste and agricultural wastewater.
- the saprophytic fungi have also proved to be efficient digestors for potentially harmful organisms such as coliform bacteria and nematodes.
- the voracious Austempilze (Pleurotus ostreatus) have proven to be parasitic to nematodes. Extracellular enzymes act like an anesthetic and numb the nematodes, allowing for the penetration of the mycelium directly into their immobilized bodies.
- the fungal material may also be derived from parasitic and / or saprophytic and / or symbiotic (endo- or ectomycorrhizal) fungi.
- Typical methods for applying fungal spores and hyphae to the soil include carriers such as cereals, sawdust and wood shavings, alginate hydrogels with and without additional nutrient sources, vermiculite and peat optionally saturated with nutrient broths, vermiculite and rice flour, or cereal flour, straw or other agricultural waste products. which are overgrown with mushroom mycelium pelleted
- Wood chips are typically inoculated by spreading mycelium impregnated sawdust and / or wood chips over the wood chips.
- Compost bunkers are typically similarly inoculated with cereal impregnated with mycelium, although in some cases also impregnated with mycelium
- Trees, lawns and seedbeds were inoculated with mycorrhiza species using tablets or gels made from spores or mycelium. Trees may also be vaccinated with mycorrhizal fungi by dusting the roots of seedlings with spores or fungal mycelium or by dipping the exposed roots of seedlings in water enriched with spores of the mycorrhizal species.
- Another method of vaccinating mycorrhizae requires the planting of young seedlings near the root zones of proven fungus-producing trees, whereby the seedlings can become infected with the mycorrhiza of an adjacent tree. After a few years, the new trees are dug up and transplanted. Another method involves sending spore mass to the root zones of trees. Such approaches are labor intensive, expensive, unsafe, and / or unsuitable for widespread use.
- the fungus is often in an encapsulated form for such applications in a matrix consisting of polymers (alginate salts, carrageenan salts, iota-carrageenan salts, maltodextrin, corn starch, modified starch, whey protein concentrate, skimmed milk powder, dry yeast autolysate,
- polymers alginate salts, carrageenan salts, iota-carrageenan salts, maltodextrin, corn starch, modified starch, whey protein concentrate, skimmed milk powder, dry yeast autolysate,
- the object of the present invention is therefore to enable a simple, sterile, cost-effective and rapid production, storage and use of fungal inoculum.
- a substrate mixture according to claim 1 is suitable for the production of a fungal culture or for the storage, transport or application of fungal material, such as fungal spores, fungal mycelium and hyphae, thus enabling the treatment of cellulosic, hemicellulose and / or lignin-based materials, terrestrial and Cultivated land, of agricultural and forestry waste, of plants and of wood, bioaugmentation, bioremediation or biological
- the substrate mixture for fungal material contains water, a binder and nanocellulose.
- Cellulose with structures in the nanometer range is called nanocellulose.
- the mean length of these cellulose particles is from 100 nm to several micrometers.
- Nanocellulose is widely differentiated on the basis of size and manufacturing method in nano- and microfibrillated cellulose (NFC and MFC), nanocrystalline cellulose (NCC), and bacterial nanocellulose (BNC).
- NFC and MFC nano- and microfibrillated cellulose
- NFC and MFC nano- or microfibrils or simply referred to as fibrillated cellulose
- Nanocrystalline cellulose sometimes called cellulose nano or microcrystals, cellulose crystallites, cellulose whiskers, rod-shaped cellulose, is obtained by partial hydrolysis of classical cellulose.
- bacterial nanocellulose also referred to as bacterial cellulose, microbial cellulose or biocellulose, by the biochemical polymerization of low molecular weight building blocks (eg, alcohols and sugars, such as glucose).
- the sources of nanocellulose are wood, sugar beet, potato tuber, hemp, flax, wood, cotton, wheat straw, mulberry bark, ramie, avicel, tunicin, cellulose from algae and bacteria, and generally wood pulp, paper pulp, plant pulp or any other biological pulp
- Nanocellulose serves as an energy or carbon source for the fungi.
- the nanocellulose influences the theological properties of the substrate mixture and acts as a gelling agent.
- the nanocellulose is selected from the group consisting of nano- and micro-fibrillated cellulose (NFC and MFC), nanocrystalline cellulose (NCC), and bacterial nanocellulose (BNC) or mixtures thereof.
- the content of nanocellulose in the substrate mixture is preferably 0.5 to 20 wt .-% and particularly preferably 1 to 15 wt .-%.
- the binder serves to adjust the rheological properties of the substrate mixture (gelling agent, thickener).
- the binder is not particularly specified and can be selected by the user according to the requirements of the substrate mixture.
- the binder should preferably be ecologically safe, i. biodegradable and in particular compatible with the fungi to be used.
- Suitable binders are alginate, agar-agar, carrageenan, locust bean gum, guar gum, tragacanth, gum arabic, xanthan, gellan, pectin, modified starch, alkylcellulose, hydroxyalkylcellulose, carboxyalkylcellulose and their alkali, alkaline earth and ammonium salts, poly (meth) acrylic acid and theirs Alkali,
- Alkaline earth and ammonium salts polyvinyl alcohol, polyacrylamide, polyvinylpyrrolidone, polyethylene glycol and mixtures thereof.
- Particularly preferred binders are alkali, alkaline earth and ammonium salts of Polyacrylic acid and most preferably the binder is sodium polyacrylate.
- the content of binder may be selected according to the wishes of the user or the requirements of the substrate mixture in use, for example to adjust a desired viscosity.
- Suitable contents of binder in the substrate mixture are from 0.1 to 1.0% by weight, preferably from 0.2 to 0.8% by weight or particularly preferably from 0.3 to 0.5% by weight.
- the substrate mixture may further contain other conventional additives to promote the growth of the fungi, such as nutrients such as organic or inorganic
- Suitable nitrogen sources are, for example, ammonium salts, nitrates or urea, it being possible for the contents to be from 0.05 to 0.2% by weight.
- glucose and glycerol may be used as the further carbon source, with preferred contents being from 0.2 to 0.4% by weight.
- malt extract preferably 0.1 to 4% by weight
- yeast extract preferably 0.1 to 0.5% by weight
- peptone preferably 0.1 to 1% by weight
- TBS tryptone soya broth
- a fungal culture according to the invention contains the substrate mixture according to the invention and fungal material.
- the fungal material may be fungal spores, fungal mycelia or hyphae.
- the fungal material may be derived from saprophytic fungi and, in particular, wood decomposing fungi.
- the fungi are species of the department Ascomycota and
- Basidiomycota Particularly suitable candidates are, for example, Physisporinus vitreus and Kretzschmaria deusta.
- the substrate mixture according to the invention can be used for producing a fungal culture or for storage, transport or application of fungal material (fungal spores, fungal mycelia or hyphae).
- the binder and the nanocellulose are simply dissolved or suspended in water.
- the substrate mixture thus obtained is then added with the fungal material (fungal spores, fungal mycelium or hyphae) to obtain the fungal culture of the present invention.
- the fungal culture thus obtained can be further incubated to grow the fungi.
- a slurry of nanocellulose optionally with the other additives described above, may be added to the fungal material and this slurry incubated to grow the fungi first and then the binder is added.
- nanocellulose in the fungal culture is at least partially digested by the fungus.
- the fungal culture according to the invention can be used for the treatment of cellulose, hemicellulose and / or lignin-based materials, soil and culture soil, agricultural and forestry waste, plants and wood, for bioaugmentation, bioremediation or biological pest control.
- the method of treating cellulose, home cellulose and / or lignin based materials, soil and crop, agricultural and forestry wastes, plants and wood, for bioaugmentation, bioremediation or biological pest control includes application a fungal culture of the invention to the object to be treated.
- Kretzschmaria deusta was cultured for 2 weeks in Petri dishes on 2% MEA. Mycelium from actively growing cultures was then used to become a medium which contained 100 g of nanocellulose, 1.5 g of malt extract and 150 ml of sterilized water. It was incubated for 2 weeks at 24 ° C and 65% relative humidity. Thereafter, the colonized nanocellulose was mixed with a blender at 5000 rpm for 30 seconds. Then, 250 g of the mycelial dispersed nanocellulose were mixed under sterile conditions with 1 g of sodium polyacrylate and 500 ml of sterile water.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102018105329.0A DE102018105329A1 (en) | 2018-03-08 | 2018-03-08 | Substrate mixture for mushroom material, mushroom culture and their use |
PCT/EP2019/052989 WO2019170355A1 (en) | 2018-03-08 | 2019-02-07 | Substrate mixture for fungal material, fungal culture and use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3606330A1 true EP3606330A1 (en) | 2020-02-12 |
Family
ID=65352032
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19703997.7A Withdrawn EP3606330A1 (en) | 2018-03-08 | 2019-02-07 | Substrate mixture for fungal material, fungal culture and use thereof |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP3606330A1 (en) |
DE (1) | DE102018105329A1 (en) |
EC (1) | ECSP20052411A (en) |
WO (1) | WO2019170355A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102020006452A1 (en) | 2020-10-20 | 2022-04-21 | Hanffaser Uckermark eG | Product of a mushroom mycelium fastening adapter and use in a wall insulation |
CN112400609A (en) * | 2020-11-25 | 2021-02-26 | 东平县科海菌业有限公司 | Edible fungus strain culture medium containing air-dried branch strains and preparation method |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10400128B2 (en) * | 2013-03-14 | 2019-09-03 | Oregon State University | Nano-cellulose edible coatings and uses thereof |
WO2015180624A1 (en) * | 2014-05-27 | 2015-12-03 | Novozymes A/S | Methods for mushroom cultivation |
CN106119123A (en) * | 2016-06-27 | 2016-11-16 | 合肥福泉现代农业科技有限公司 | A kind of white beech mushroom liquid spawn culture medium based on bean curd yellow pulp water and white beech mushroom liquid spawn preparation method |
-
2018
- 2018-03-08 DE DE102018105329.0A patent/DE102018105329A1/en not_active Withdrawn
-
2019
- 2019-02-07 WO PCT/EP2019/052989 patent/WO2019170355A1/en unknown
- 2019-02-07 EP EP19703997.7A patent/EP3606330A1/en not_active Withdrawn
-
2020
- 2020-08-26 EC ECSENADI202052411A patent/ECSP20052411A/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2019170355A1 (en) | 2019-09-12 |
ECSP20052411A (en) | 2020-09-30 |
DE102018105329A1 (en) | 2019-09-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11427513B2 (en) | Growth media for improved growth and yield of fungus using treated lignocellulosic biomass | |
CN105123008B (en) | Method assembling straw biochar with microbe bacteria fertilizer so as to improve saline land | |
CN101759494B (en) | Diseases-prevention organic fertilizer prepared by edible fungi residue | |
CN103787775A (en) | Eucalyptus seedling growing and cultivation medium and preparation method thereof | |
EP1739067B1 (en) | Process for manufacturing a soil conditioner | |
JP5796843B2 (en) | Compost production method for fermenting pruned branches and leaves to compost | |
WO2019170355A1 (en) | Substrate mixture for fungal material, fungal culture and use thereof | |
CN1078192C (en) | Bio-bacterial agent and its preparing process | |
US6506956B1 (en) | Mold capable of degrading dioxin, degradation of dioxin with the use of the same, method for producing composts capable of degrading dioxin and method for growing plants | |
CN109609132B (en) | Soil remediation liquid and using method thereof | |
EP3630706B1 (en) | Long-term humus water storage hybrid | |
CN105254347A (en) | Production method of organic fertilizer | |
DE102012101786B4 (en) | Method of cultivating a mushroom spawn | |
JP2539256B2 (en) | Method for manufacturing compost from wood resources | |
Wani et al. | Studies on biological decomposition of wheat straw: I. Screening of wheat straw decomposing micro-organisms in vitro | |
DE3781936T2 (en) | MUSHROOM CULTIVATION. | |
Setiawati et al. | The effectiveness of various compositions lignolytic and cellulolytic microbes in composting empty fruit bunch palm oil and sugar cane biomass | |
CN1274701A (en) | Method for treatment of hair and feather using microbe | |
CN106399126A (en) | Method for restoration of carbofuran and chlorpyrifos pesticide-polluted soil through immobilized white-rot fungi | |
Njoku et al. | Lignocellulose degradation and crude protein formation by three ligninolytic Streptomyces strains | |
DE19845508C2 (en) | Fermentation residue decomposition | |
KR102499785B1 (en) | Functional biochar containing sulfur | |
EP4083188A1 (en) | Bacterial bioproduct, method for its manufacture and application | |
DE69031659T2 (en) | INOCULUM OF ECTOMYCORRHIZA MUSHROOMS THAT CAUSE AN ENDOMYCORRHIZA INFECTION IN GRASSES | |
DD154930A3 (en) | GROWTH STIMULATOR FOR CHAMIGNON GROWTH |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: UNKNOWN |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20191107 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
AX | Request for extension of the european patent |
Extension state: BA ME |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
|
17Q | First examination report despatched |
Effective date: 20200316 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
|
DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20210407 |