EP3602164A1 - Unité de commande programmable pour microscope pourvue de raccordements de libre utilisation, système microscope doté d'une unité de commande de microscope et procédé de fonctionnement d'une unité de commande de microscope - Google Patents

Unité de commande programmable pour microscope pourvue de raccordements de libre utilisation, système microscope doté d'une unité de commande de microscope et procédé de fonctionnement d'une unité de commande de microscope

Info

Publication number
EP3602164A1
EP3602164A1 EP18727150.7A EP18727150A EP3602164A1 EP 3602164 A1 EP3602164 A1 EP 3602164A1 EP 18727150 A EP18727150 A EP 18727150A EP 3602164 A1 EP3602164 A1 EP 3602164A1
Authority
EP
European Patent Office
Prior art keywords
control unit
microscope
script
microscope control
components
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18727150.7A
Other languages
German (de)
English (en)
Inventor
Markus Weber
Robert Mainberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
Original Assignee
Leica Microsystems CMS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of EP3602164A1 publication Critical patent/EP3602164A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B19/00—Programme-control systems
    • G05B19/02—Programme-control systems electric
    • G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
    • G05B19/042—Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
    • G05B19/0426—Programming the control sequence
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B2219/00—Program-control systems
    • G05B2219/20—Pc systems
    • G05B2219/26—Pc applications
    • G05B2219/2656—Instrumentation
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B2219/00—Program-control systems
    • G05B2219/30—Nc systems
    • G05B2219/45—Nc applications
    • G05B2219/45121—Operating microscope, mounted on manipulator arm

Definitions

  • the present invention relates to a microscope control unit having at least one connection which can be connected to one or more electrically controllable microscope components or other electrically controllable components, and to an automated microscope system having such a microscope control unit and to a method for operating such a microscope control unit.
  • microscopes with one or more electrically controllable components are referred to, for example, where a digital camera acts as a detector and the images are displayed on a monitor or, for example, the light source is controlled. It can also be a digital microscope, which is characterized by the fact that it dispenses with a tube for a visual insight on the microscope image and generates only a digital microscope image and displays this on a display or monitor.
  • Such automated microscopes usually have a microscope control unit, which comprises an electronic unit and a control program, the so-called firmware.
  • firmware for every microscope component, which should be electrically controllable, a program part be present. This applies, for example, to components such as camera, x / y stage, zoom, lighting, etc.
  • the microscope control unit can be connected to input devices, such as rotary knobs, switches or touchscreens, but also to a user interface on conventional PCs, which runs by means of a so-called human machine interface (HMI software for human machine interface) ,
  • DE 10 2010 063 392 A1 shows a digital microscope, which dispenses with visual inspection, and with an image acquisition device set up for the optical and digital acquisition of an object to generate an object image, and with a display of the object image in a display region and for recording equipped with inputs in the display area formed sensor screen, wherein the microscope for changing settings of motorized and / or electrically controllable microscope components on the microscope is set up based on the detected in the display area of the touch screen inputs.
  • microscope systems have a microscope control unit, associated electrically controllable microscope components and a computer (PC) for operation.
  • the PC can also be used to offer the user, in a software program, automated sequences and boundary conditions for selection, which are then executed by the microscope control unit. As a result, processes that are not offered can not be executed.
  • EP 1 426 754 A1 relates to the control of an image recording, in which a control unit controls the devices required for image recording and a computer unit processes the data of the recorded images. To get the speed To increase the flexibility and reproducibility of image acquisition, it is proposed to combine control commands for image acquisition into at least one script and to transmit at least one script from the computer unit to the control unit.
  • a microscope control unit with at least one connection which can be connected to one or more electrically controllable microscope components or other electrically controllable components
  • a microscope system with such a microscope control unit and a method for operating such a microscope control unit with the features of the independent patent claims are proposed.
  • Advantageous embodiments are the subject of the dependent claims and the following description.
  • a microscope control unit with at least one connection which is connectable to one or more electrically controllable microscope components or other electrically controllable components, presented, wherein at least one connection parameter of the at least one terminal programmatically configurable, wherein on the microscope control unit at least one Script is stored with script commands, and wherein the at least one port is controlled by means of these script commands.
  • a microscope control unit has the advantage that the at least one connection can be freely used by the user.
  • connection refers to an electrical interface serving as inputs or outputs for electrical control signals to electrically controllable components and / or electrically controllable components, which are arranged inside or outside the microscope system, are definable.
  • Free means, in particular, that the connection in question does not serve a predetermined purpose, but is freely usable and, in particular, freely definable in its function or protocol, so that the connection can be used in a predefined way by means of appropriate script commands
  • any electrically controllable microscope components or other components operated in conjunction with the microscope can be controlled by the microscope control unit when executing the script.
  • the microscope control unit can be set up to control any combination of one or more of the following electrically controllable microscope components by means of the script commands via the at least one connection:
  • a motorized Z-drive for adjusting the distance between a lens and a sample
  • a motorized turntable with optical components for example an incident light turntable (IL Turret) with a plurality of optionally insertable into the beam path of the microscope Fluorenzfilter cubes, an electrically switchable light source,
  • IL Turret incident light turntable
  • AOBS acousto-optic beam splitter
  • AOM acousto-optic modulator
  • the microscope control unit can be configured to control other electrically controllable components by means of the script commands.
  • electrically controllable components are conceivable which can be operated in conjunction with the microscope and a test carried out therewith.
  • the experimental setup around the microscope may require a controllable climate chamber or a water supply or discharge or a gas supply or discharge (eg evacuation to a certain negative pressure).
  • an electrically controllable water pump or gas pump or vacuum pump or an electrically controllable climate control unit could be controlled by the microscope control unit via the at least one connection.
  • the invention offers the advantage that the user of the microscope himself can select which electrically controllable components he wants to use and couple to the microscope.
  • This free configuration of the microscope with microscopically adapted to the microscope microscope components and the control of these microscope components is independent of whether they were already known at the time of manufacture of the microscope or routinely supported by the firmware of the manufacturer of the microscope control unit or not.
  • a disadvantage of the earlier solution lies in the fact that each component to be controlled had to be permanently implemented in the microscope control unit.
  • every smallest extension of the experimental set-up caused a change in the microscope control unit due to a newly used, electrically controlled component. For example, a new camera type with new trigger behavior inevitably required a factory change to the microscope control unit.
  • the at least one connection parameter of the at least one connection can be configured programmatically by means of the script commands.
  • a configuration environment may be provided in the form of a user program, for example in the form of the programming device or PC mentioned below.
  • the at least one programmably configurable connection parameter preferably comprises at least one parameter which configures the connection as:
  • the at least one programmably configurable connection parameter preferably comprises at least one parameter selected from:
  • the configuration may include any combination of parameters.
  • the at least one connection comprises an input and is optionally configurable as an analog input or as a digital input.
  • special connections designed as analog inputs and special connections designed as digital inputs may also be included and configurable. Such an embodiment is easier to manufacture.
  • the at least one port comprises an output and is optionally configurable as an analog output or as a digital output.
  • special connections designed as analog outputs and special digital outputs are also included and configurable.
  • the at least one port comprises a so-called general purpose input / output (GPIO) port. It is then a connection whose behavior, independently of whether as input or output, is freely programmable. Such an embodiment offers great flexibility.
  • GPIO general purpose input / output
  • the microscope control unit is configured to be able to process script instructions of a predetermined script instruction set.
  • the script command set also includes script commands which are assigned no functions of the at least one port, but other functions, in particular program flow operations (such as waiting, repeating, loops, etc.), arithmetic operations (such as adding, multiplying, etc.), etc.
  • the script commands of the at least one script can be selected from the script command record by a user, and their sequence in the script can be specified by the user.
  • the user can make the script substantially free using the instruction set.
  • the microscope control unit is set up to be data-transmitting connected to a user interface via which the user can select the script commands of the at least one script from the script command set and via which the sequence in the script can be predetermined by the user.
  • the microscope control unit can be designed such that the user interface is designed as a hardware-based input unit of a programming device.
  • the user interface is designed as a user interface of a running on a separate processing unit, in particular PC, user program.
  • the user program can be a (pure) programming environment or an operating program for the microscope system.
  • the microscope control unit is preferably configured to be connected to a PC for data transmission.
  • HMI software can be executed which serves as a user interface by being implemented as a user interface of the user program. From there, the transmission of the script to the microscope control unit, by means of which the microscope system is operated. This makes the programming and / or operation of the microscope system very convenient and easy.
  • the PC can also be used for visualization, for example of recorded images or of any measured values.
  • the script may also be stored in a volatile memory, for example in a cache memory, or in a non-volatile memory of the operating PC or the microscope control unit or an external memory or a server.
  • a volatile memory for example in a cache memory, or in a non-volatile memory of the operating PC or the microscope control unit or an external memory or a server.
  • Another variant of the microscope control unit can be designed so that the script can be transferred as a text file to the microscope control unit. It is advantageous if the script is received in a text format, for example ASCII, ANSI or Unicode / UTF, since this ensures great compatibility.
  • the microscope control unit is designed for fast and user-friendly control of the at least one connection by assigning it a control program with an interpreter for the script, which converts the script commands into functions of the at least one connection.
  • a control program with an interpreter for the script, which converts the script commands into functions of the at least one connection.
  • This provides the ability to conveniently transfer the script to the microscope control unit in an intelligible or readable form, i. as a sequence of textual script commands.
  • a compilation step before transfer can be omitted, so that in particular the programming device does not have to be adapted for compilation for different target systems.
  • This advantage is achieved because the interpreter contained in the control program is not part of the user program.
  • the control program is stored in the microscope control unit itself.
  • the control program is stored in an assembly outside the microscope control unit.
  • the microscope control unit has an electronics unit and comprises the associated control program with an interpreter for the script.
  • the microscope control unit according to the invention not only allows substantially simpler adaptations of the functional assignment of the at least one connection to newly adapted electrically controllable microscope components or other electrically controllable components of the experimental setup.
  • the script commands are implemented as real-time commands for controlling the at least one connection.
  • a plurality of functional assignments of the at least one connection of the microscope control unit can be realized.
  • a channel type for the at least one port can be defined for the microscope control unit by means of the script commands.
  • At least one variable and / or at least one command and / or at least one script and / or at least one function block be provided by means of channels in the microscope control unit by means of the script commands.
  • FIG. 1 shows schematically in a block diagram a microscope system according to a preferred embodiment of the invention.
  • Figure 2 shows schematically the microscope system of Figure 1 in another schematic view.
  • FIG. 3 shows schematically in a block diagram a sequence of an exemplary experiment using a microscope system according to the invention.
  • FIG. 4 schematically shows, in a block diagram, the course of the experiment when using a microscope system according to a preferred embodiment of the invention.
  • FIGS. 1 and 2 schematically show a microscope system according to a preferred embodiment of the invention.
  • the microscope system has a microscope control unit 100 according to a preferred embodiment of the invention in a running on the microscope control unit 100 script for the use of freely usable connections in the form of inputs and outputs 101, with which one configured here as a control PC 10
  • HMI Human / machine interface
  • microscope components 200, 300 are signal transmitting connected.
  • the microscope components can be subdivided, for example, into internal microscope components 300 and external microscope components 200.
  • the internal microscope components 300 are characterized in that they are mounted on or in the microscope chassis 1 and are considered as part of the microscope, such as an IL-turret 301, a Z-drive 302, a reivrevverver 303, etc.
  • external microscope components 200 for example, an XY table 201, a lighting device (possibly with shutter) 202, a Camera 203, etc. are viewed.
  • these may also be mounted on the microscope chassis 1, they are usually not regarded as part of "the microscope”.
  • the microscope components 200, 300 each have a controllable actuator (for example electric motor), they can be controlled in the context of the invention in a script running on the microscope control unit 100.
  • the script comprises a sequence of selected script commands to which functions of the inputs and outputs of the microscope control unit 100 are assigned. Such functions include, in particular, reading and writing functions.
  • microscope master The microscope master can be implemented by a microcontroller, DSP (Digital Signal Processor) or FPGA (Field Programmable Gate Array) ,
  • Some components have dependencies on other components. For example, it may be necessary for the Z-drive to be lowered prior to moving the nosepiece to prevent a collision of lenses with the XY-table.
  • the dependencies which are absolutely necessary to ensure operational safety, are referred to as critical dependencies and are preferably implemented firmly in the microscope master. That When a movement of the nosepiece is controlled, the microscope master automatically controls the Z-drive accordingly. This can include lowering the XY table, then rotating the nosepiece and, if necessary, lifting the XY table.
  • the configuration preferably takes place via HMI, for example PC 10, where the connections to be used are declared to the components to be controlled and the dependencies of the connections to one another be configured.
  • HMI for example PC 10
  • the configuration can be done completely or in parts at runtime.
  • each port receives a unique number, the channel number.
  • channel numbers are assigned to the inputs and / or outputs of the microscope control unit 100 by appropriate script commands.
  • each electrically controllable microscope component can be assigned at least one channel, wherein each channel in the script is configured by appropriate script commands as input and / or output for sequential or parallel processing.
  • the channel number is used to access the script, which is designed as part of the control program of the microscope control unit, that is to say of the microscope master 100.
  • the script defines the program flow using channel numbers.
  • the available script instructions are fixedly implemented in the microscope master 100, here preferably in an interpreter running on the control program of the microscope master 100, and provide rudimentary instructions, for example 'writing', 'reading', 'adding' or 'multiplying' ren ', which are used in particular for processing the defined channels.
  • the available set of instructions is called a instruction set.
  • the microscope master can be set up to be able to process one or more of the following commands, to which functions of the inputs and outputs of the microscope control unit 100 are partially assigned.
  • - SET CHANNEL Sets the value of a channel.
  • WAIT CHANNEL Waits until the specified value is reached.
  • the microscope control unit 100 that is, the microscope master, executes scripts in a timer with temporal grid, wherein the time interval for the temporal grid is freely selectable.
  • This offers a real-time capability for the running control program of the microscope control unit 100.
  • Real-time systems are characterized by the fact that a result of an arithmetic operation (eg process or task) is guaranteed within a defined time interval, ie before a certain time limit.
  • a so-called "real-time” system runs on the processor of the microscope control unit 100 for this purpose.
  • Operating system that regulates the different processes and tasks.
  • Alternative solutions are also known which do not require a real-time operating system. This is made possible, for example, by the use of state machines (statemachines) and interrupts.
  • scripts and the number of script commands defined therein are not limited or only by the memory location of the microscope control unit 100, that is, the microscope master, according to the preferred embodiment described here.
  • scripts can be started and stopped, for example by user input from the PC 10.
  • the script itself also has an interface and a freely definable channel number, as a result of which a script can be viewed like any other channel with all available operations.
  • the microscope control unit 100 is configured to provide different types of channels, such as e.g. one or more of type 'variable', 'hardware', 'command', 'script' and 'function block'.
  • types of channels such as e.g. one or more of type 'variable', 'hardware', 'command', 'script' and 'function block'.
  • Each instance of a channel type is assigned a unique channel number.
  • a variable can be written and read.
  • Hardware A hardware channel is any analog or digital input or output. This channel type is used, for example, to trigger external components. For synchronization with an external component, such as a camera, for example, a digital input hardware channel and a digital output hardware channel are defined. Using both channels, trigger conditions can be implemented. Another example is generating any output voltage to control an external component, such as a light source. The value of the output voltage is set out of a script.
  • Command A command describes an ASCII string for communication with a component. The ASCII string to be used is defined prior to use and can have both a static and a dynamic part, with the dynamic part always being assigned to the variable type. The dynamic component can be used, for example, to set the position of a component.
  • the string "76022 position” is used to set the position of the nosepiece, where the position can be either dynamic or static.
  • a script consists of any number of sequential commands for processing channels.
  • a function block describes the linking of channels, whereby the function can be a logical, for example "AND” or “OR” or arithmetic "ADD” or “MULTIPLIER” as well as a permanently defined or freely definable LUT (look-up table).
  • the microscope master first the components and parameters to be used are selected (during the programming) and the time sequence is configured.
  • the selection and configuration of the script commands in the desired order, so the definition of the script for example, via the PC 10 in a dedicated input menu.
  • This input menu represents the user interface, which is designed as a user interface of an application program, with which desired script commands can be selected from the script command set and whose sequence can be specified in the script.
  • the sequence of an experiment is shown from the components XY table 301, illumination 302 and camera 303. This process is repeated n times within an experiment.
  • the process includes (after configuration / declaration), for example, the following steps:
  • the interfaces or channel numbers of the components may look like this, for example:
  • the script can look like this:
  • a disadvantage of such a solution is that in a control program running on the microscope master no access to certain microscope components, such as e.g. XY table control, Z-drive, IL-turret is possible. These must be controlled by a user program running on the PC so that no real-time experiment is possible.
  • the type and number of electrically controllable components is limited by the firmware specified by the manufacturer of the microscope system or the microscope control unit.
  • PC sets command to move from XY table to microscope.
  • PC sets command to move IL-Turret to microscope.
  • the microscope control unit 100 Since the microscope control unit 100, the microscope master, is set up to execute a script with script commands and assign the script commands selectable functions of the inputs and outputs by means of the interpreter in the control program of the microscope control unit 100, all steps of the experiment in the context of a on the Microscope control unit 100 running control program to be performed.
  • the user sets the script with the script commands for assigning the channels before the experiment. It also configures the ports to use, such as the protocol to use, and so on.
  • the script prepared by the user contains the script commands selected in the order selected. By means of the interpreter of the control program, the script is converted into commands for configuring the connections, for determining the channels of the inputs and outputs of the microscope control unit 100, and thus for commands for controlling all electrically controllable components. This starts the execution as a real-time experiment.
  • PC transmits the script prepared by the user to the microscope control unit 100, the microscope master
  • the commands in the form of the script commands in real time are now available to the microscope control unit 100 and thus to the microscope system through the script which can be freely configured by the user before the experiment. This leads to a much faster course of the experiment.
  • the user can also integrate any electrically controllable components into his experiment.

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Multimedia (AREA)
  • General Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Automation & Control Theory (AREA)
  • Microscoopes, Condenser (AREA)
  • Programmable Controllers (AREA)

Abstract

La présente invention concerne une unité de commande de microscope (100) comprenant au mois un raccordement (101) apte à être relié à un ou plusieurs composants de microscope (201, 202, 203, 204, 301, 302, 303, 304) à commande électrique ou d'autres composants à commande électrique, au moins un paramètre de raccordement de l'au moins un raccordement (101) pouvant être configuré par un système de programmation, au moins un script avec erreurs de script étant fourni au niveau de l'unité de commande de microscope (100), et l'au moins un raccordement (101) pouvant être commandé à l'aide des erreurs de script. L'invention concerne également un système de microscope équipé d'une telle unité de commande de microscope (100) et d'au moins un composant de microscope ou d'autres composants à commande électrique.
EP18727150.7A 2017-04-28 2018-04-26 Unité de commande programmable pour microscope pourvue de raccordements de libre utilisation, système microscope doté d'une unité de commande de microscope et procédé de fonctionnement d'une unité de commande de microscope Withdrawn EP3602164A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102017109252.8A DE102017109252A1 (de) 2017-04-28 2017-04-28 Programmierbare Mikroskopsteuerungseinheit mit frei verwendbaren Ein- und Ausgängen, Mikroskopsystem mit einer Mikroskopsteuerungseinheit und Verfahren zum Betrieb einer Mikroskopsteuerungseinheit
PCT/EP2018/060714 WO2018197609A1 (fr) 2017-04-28 2018-04-26 Unité de commande programmable pour microscope pourvue de raccordements de libre utilisation, système microscope doté d'une unité de commande de microscope et procédé de fonctionnement d'une unité de commande de microscope

Publications (1)

Publication Number Publication Date
EP3602164A1 true EP3602164A1 (fr) 2020-02-05

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EP18727150.7A Withdrawn EP3602164A1 (fr) 2017-04-28 2018-04-26 Unité de commande programmable pour microscope pourvue de raccordements de libre utilisation, système microscope doté d'une unité de commande de microscope et procédé de fonctionnement d'une unité de commande de microscope

Country Status (6)

Country Link
US (1) US11550137B2 (fr)
EP (1) EP3602164A1 (fr)
JP (1) JP2020518027A (fr)
CN (1) CN110573926B (fr)
DE (1) DE102017109252A1 (fr)
WO (1) WO2018197609A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3882771A1 (fr) * 2020-03-16 2021-09-22 Leica Microsystems CMS GmbH Système de commande et procédé de fonctionnement d'un système

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JP2002290490A (ja) 2001-03-28 2002-10-04 Sony Corp 電子機器
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DE10256706A1 (de) * 2002-12-04 2004-07-08 Leica Microsystems Wetzlar Gmbh Verfahren zur Steuerung einer Bildaufnahme und Steuereinrichtung hierfür
JP2007150697A (ja) * 2005-11-28 2007-06-14 Ricoh Co Ltd 通信装置、通信制御方法、通信制御プログラム及び記録媒体
JP4620035B2 (ja) * 2006-12-11 2011-01-26 三菱電機株式会社 プログラム作成装置、プログラム作成方法およびその方法をコンピュータに実行させるプログラム
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DE102010063392B4 (de) 2010-11-15 2016-12-15 Leica Microsystems (Schweiz) Ag Mikroskop mit Sensorbildschirm, zugehörige Steuereinrichtung und Betriebsverfahren
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US11550137B2 (en) 2023-01-10
CN110573926B (zh) 2023-02-17
WO2018197609A1 (fr) 2018-11-01
DE102017109252A1 (de) 2018-10-31
CN110573926A (zh) 2019-12-13
JP2020518027A (ja) 2020-06-18
US20210116695A1 (en) 2021-04-22

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