EP3589405A1 - Dispositifs microfluidiques améliorés et leurs procédés d'obtention - Google Patents

Dispositifs microfluidiques améliorés et leurs procédés d'obtention

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Publication number
EP3589405A1
EP3589405A1 EP18707718.5A EP18707718A EP3589405A1 EP 3589405 A1 EP3589405 A1 EP 3589405A1 EP 18707718 A EP18707718 A EP 18707718A EP 3589405 A1 EP3589405 A1 EP 3589405A1
Authority
EP
European Patent Office
Prior art keywords
polymeric
layer
barrier coating
coating layer
polymeric device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18707718.5A
Other languages
German (de)
English (en)
Inventor
Michael Haupt
Peter Loskill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Publication of EP3589405A1 publication Critical patent/EP3589405A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
    • B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
    • B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
    • B81C1/00206—Processes for functionalising a surface, e.g. provide the surface with specific mechanical, chemical or biological properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B32—LAYERED PRODUCTS
    • B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
    • B32B27/00—Layered products comprising a layer of synthetic resin
    • B32B27/28—Layered products comprising a layer of synthetic resin comprising synthetic resins not wholly covered by any one of the sub-groups B32B27/30 - B32B27/42
    • B32B27/283—Layered products comprising a layer of synthetic resin comprising synthetic resins not wholly covered by any one of the sub-groups B32B27/30 - B32B27/42 comprising polysiloxanes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/058—Microfluidics not provided for in B81B2201/051 - B81B2201/054

Definitions

  • the present invention relates to coated polymeric devices, in particular microfluidic devices, cell culture devices and membranes, processes for preparing them and uses thereof.
  • Microfluidic devices are characterised by a network or system of microchannels arranged, in particular moulded or patterned in a substrate. They are characterised by a small size and very small sample volumes in femto-, pico-, nano- or ⁇ -range.
  • Microfluidic devices thus, allow using extremely small volumes of samples, they allow carrying out complex biochemical reactions for obtaining biological, medical and chemical information, they have the advantage to reduce the required amount of samples, they shorten the response time of reactions, they decrease the amount of bio hazard and waste for disposal and they safe money.
  • microfluidic devices are for use in lab-on-a-chip and organ-on-a-chip systems, allowing testing of potential drugs, cosmetic agents, dyes, biomarkers or other molecules characteristics which can be tested on tissues or cells.
  • microfluidic devices were fabricated in silicon and glass using photolithography and etching techniques adapted from the microelectronic industry.
  • microfluidic devices are made from plastic, polymeric materials and glass.
  • Polymeric materials frequently used for the construction of microfluidic devices include PDMS (polydimethylsiloxane). These materials allow to design microfluidic devices with improved specific optical characteristics, biological or chemical compatibility, faster prototyping, lower production costs and they allow electrosensing.
  • US 2012/0181 184 A 1 discloses microfluidic devices comprises porous hydrophilic layers for the analysis of various analyst in a sample.
  • US 2012/0168010 discloses methods of layer-by- layer deposition of a plurality of layers of material onto the wall of a channel of a microfluidic device, involving injecting solutions into the microfluidic device.
  • one major problem polymeric devices in particular cell culture devices, membranes and microfluidic devices, frequently encounter is partitioning of molecules, in particular hydrophobic molecules, from the sample contained on the surface of the polymeric device, for instance in the microchannels of a microfluidic device, into the polymeric material forming the polymeric device, for instance the microfluidic device.
  • the absorption of said molecules into the polymeric device, in particular the microfluidic device causes manifold problems.
  • the technical problem underlying the present invention is to provide a polymeric device, in particular microfluidic device, cell culture device or membrane, and a process for producing it, overcoming the above identified technical problems, in particular which shows no or a reduced absorption of substances contained in a sample into the material of the polymeric device, in particular microfluidic device, cell culture device or membrane.
  • the present invention solves the above identified problem by providing a coated polymeric device comprising at least on a part of the surface of a polymeric device an absorption barrier layer system, wherein the absorption barrier layer system comprises at least one polymeric interface layer and at least one barrier coating layer, in particular by providing a coated polymeric device comprising a system (20) of microchannels (25), a system (20) of wells, a system (20) of pores or a combination thereof in a polymeric device (10), wherein at least a part of the surface of the microchannels (25), wells or pores is formed by an absorption layer system (30), comprising at least one polymeric interface layer (40) and at least one barrier coating layer (50), wherein a first barrier coating layer (50) is deposited on the surface of the polymeric device (10) or on a first polymeric interface layer (40), which is deposited on the surface of the polymeric device (10) and wherein the at least one polymeric interface layer (40) and at least one barrier coating layer (50) are deposited on the polymeric device
  • the absorption barrier layer system according to the present invention advantageously prevents or reduces the diffusion of molecules, in particular hydrophobic molecules, from the sample into the material of the polymeric device, in particular microfluidic device, cell culture device or membrane, and vice versa.
  • a polymeric device is preferably a microfluidic device, a membrane or a cell culture device.
  • the polymeric device is a microfluidic device.
  • the polymeric device is a membrane.
  • the polymeric device is a cell culture device, wherein the cell culture device is preferably a matrix, a sheet, a plate, a carrier, a carrier plate or a dish.
  • the polymeric device according to the present invention comprises at least one topographical structure.
  • the topographical structure is a three-dimensional structure, for instance a system of pores, a system of microchannels or a system of wells or a combination thereof, in particular a pore, a microchannel and/or a well, wherein at least one of the dimensions of said topographical structure has an internal cross-sectional dimension (that means depth, width or length) of less than 1000 ⁇ , in particular has at least one diameter in the range from 0.1 to 990 ⁇ , preferably 0.5 to 900 ⁇ , in particular 0.5 to 800 ⁇ , preferably 1 to 400 ⁇ , preferably 1 to 10 ⁇ , preferably 1 to 5 ⁇ .
  • the polymeric device does not comprise a topographical structure that means has no system of pores, no system of microchannels and/or system of wells.
  • the present invention provides the teaching to provide an absorption barrier layer system as defined above, being present on at least a part of the surface of the polymeric device so as to provide a coated polymeric device.
  • the absorption barrier layer system may be present on the complete surface of the polymeric device.
  • the absorption barrier layer system may, however, in another embodiment be present on solely part of the polymeric device.
  • the absorption barrier layer system is present solely on the surface of the topographical structure that means in particular solely on the surface of the system of microchannels, pores, and/or wells.
  • the absorption barrier layer system is present both on the surface of the topographical structure and the residual surface of the polymeric device.
  • the absorption barrier layer system is present solely on those surface areas of the polymeric device which do not form the surface of a topographical structure.
  • the present invention solves the above identified problem by providing a microfluidic device, comprising a system of microchannels in a polymeric device, wherein at least a part of the surface of the microchannels is formed by an absorption barrier layer system, wherein the absorption barrier layer system comprises at least one polymeric interface layer and at least one barrier coating layer.
  • the at least one polymeric interface layer is deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels, and the at least one barrier coating layer is deposited on the polymeric interface layer.
  • Further preferred embodiments comprise an absorption barrier layer system which comprises 2, 3, 4, 5, 6, 7, 8, 9, 10 or more barrier coating layers each of them separated from the other by an intervening polymeric interface layer, wherein the first of these barrier coating layers is either deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels, or on a polymeric interface layer being itself deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels.
  • the at least one polymeric interface layer and the at least one barrier coating layer is deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels, which surface of the polymeric device, in particular of the pores, wells and/or microchannels, is formed by a crosslinked superficial section of the polymeric device.
  • a crosslinked superficial section of the polymeric device as referred to herein is a superficial section or area of the polymeric device which has been subjected to a particular crosslinking process step, in particular a plasma-, preferably low pressure plasma- process step involving UV- and/or VUV-radiation so as to provide an additional absorption barrier structure.
  • a crosslinking process step in particular a plasma-, preferably low pressure plasma- process step involving UV- and/or VUV-radiation so as to provide an additional absorption barrier structure.
  • the presently referred to crosslinked superficial section of the polymeric device is a particular superficial section of the polymeric device having been subjected to such a process step.
  • a crosslinking process which is performed to prepare the polymeric device from the starting material is a crosslinking referring to prepare the polymeric device itself and is not considered to relate to the formation of the particular preferred crosslinked superficial section of the polymeric device which latter has been obtained by subjecting a prepared polymeric device to a particular surface-crosslinking process, in particular plasma-based UV7VUV process for obtaining a crosslinked section solely in the superficial area of polymeric device.
  • an absorption barrier layer system which comprises 2, 3, 4, 5, 6, 7, 8, 9, 10 or more barrier coating layers each of them separated from the other by an intervening polymeric interface layer, wherein the first of these barrier coating layers is either deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels, or on a polymeric interface layer deposited on the surface of the polymeric device, in particular of the pores, wells and/or microchannels, wherein all of these layers are located on a crosslinked superficial section of the polymeric device.
  • the absorption barrier layer system comprises at least one polymeric interface layer and at least one barrier coating layer, preferably the absorption barrier layer system comprises at least one polymeric interface layer and at least one barrier coating layer and a surface of the polymeric device, in particular of the pores, wells and/or microchannels, which is a crosslinked superficial section of the polymeric device.
  • the present invention provides a coated polymeric device, in particular micro fluidic device, which device is made from a polymeric substrate, for instance polydimethylsiloxane (PDMS).
  • a coated polymeric device in particular micro fluidic device, which device is made from a polymeric substrate, for instance polydimethylsiloxane (PDMS).
  • PDMS polydimethylsiloxane
  • a system of microchannels is located in the polymeric device.
  • at least part of the surface of said microchannels, in particular the complete surface of the microchannels is formed by an absorption barrier layer system comprising at least one polymeric interface layer and at least one barrier coating layer.
  • the presently provided absorption barrier layer system is called herein also a hybrid multilayered absorption barrier layer system.
  • the present absorption barrier layer system provides a barrier for partitioning of molecules contained in a sample, for instance a sample to be tested, cultivated or analysed and being provided on the polymeric device, in particular in the pores, wells and/or microchannels, for instance of a membrane, a cell culture device or a micro fluidic device, into the material of the polymeric device itself.
  • the absorption barrier layer system of the present invention thus, provides a protection against partitioning of substances, in particular hydrophobic substances, from a sample into the material of the polymeric device.
  • the absorption barrier layer system comprises in one embodiment one or more barrier coating layers, which are deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, or on a crosslinked superficial section of the polymeric device.
  • the absorption barrier layer system comprises one barrier coating layer
  • said barrier coating layer is deposited on a polymeric interface layer, which deposited polymeric interface layer is deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, or deposited on the crosslinked superficial section of the polymeric device.
  • each of said barrier coating layers is separated from the other by an intervening polymeric interface layer, the number of which depends upon the number of the barrier coating layers to be separated from each other and wherein either the first barrier coating layer is directly deposited on the surface area of the polymeric device, in particular pores, wells and/or microchannels, or on a crosslinked superficial section of the polymeric device or wherein the first barrier coating layer is deposited on a polymeric interface layer which polymeric interface layer either is being deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, or on a crosslinked superficial section of the polymeric device.
  • a polymeric interface layer is located a) between two barrier coating layers and/or bl) between the surface of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer or b2) between the crosslinked superficial section of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer.
  • a polymeric interface layer is located a) between two barrier coating layers or bl) between the surface of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer, or a) between two barrier coating layers and bl) between the surface of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer.
  • a polymeric interface layer is located a) between two barrier coating layers or b2) between the crosslinked superficial section of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer, or a) between two barrier coating layers and b2) between the crosslinked superficial section of the polymeric device, in particular pores, wells and/or microchannels, and a barrier coating layer.
  • the absorption barrier layer system comprises at least one barrier coating layer deposited on a polymeric interface layer which polymeric interface layer is deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels.
  • the absorption barrier layer system comprises 2, 3, 4, 5, 6, 7, 8, 9, or 10, preferably 2, 3 or 4 barrier coating layers, each barrier layer separated from the other by an intervening polymeric interface layer.
  • the at least one polymeric interface layer and the at least one barrier coating layer are deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, or on a crosslinked superficial section of the polymeric device, in particular pores, wells and/or microchannels, in an alternating manner.
  • a barrier coating layer in the absorption barrier layer system is separated from a further barrier coating layer of the absorption barrier layer system by a polymeric interface layer.
  • a polymeric interface layer in the absorption barrier layer system is separated from a further polymeric interface layer in the absorption barrier system by a barrier coating layer.
  • the absorption barrier layer system of the present invention comprises a crosslinked superficial section of the polymeric device, preferably PDMS substrate, and at least one, preferably at least two, preferably at least three, preferably at least four, preferably at least five, preferably at least six, preferably at least seven, preferably at least eight, preferably at least nine barrier coating layers deposited on the polymeric device, preferably the PDMS device, in particular on a crosslinked superficial section of the polymeric device, preferably the PDMS device, and a corresponding number of intervening polymeric interface layers deposited between two adjacent barrier coating layers.
  • the absorption barrier layer system comprises one polymeric interface layer deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, and being coated with one barrier coating layer.
  • Such an absorption barrier layer system is called a single-layer system.
  • the absorption barrier layer system comprises two barrier coating layers, namely a first and a second barrier coating layer, counted from the polymeric device to the surface of the barrier system, separated by an intervening polymeric interface layer, and wherein the first barrier layer is deposited on the surface area of the polymeric device, in particular pores, wells and/or microchannels.
  • Such an absorption barrier layer system is called a double-layer system.
  • the absorption barrier layer system comprises three barrier coating layers, namely a first, a second and a third barrier coating layer, counted from the polymeric device to the surface of the barrier system, wherein between the first and the second and between the second and the third barrier coating layer each a polymeric interface layer is deposited and wherein the first barrier layer is deposited on the surface area of the polymeric device, in particular pores, wells and/or microchannels.
  • Such an absorption barrier layer system is called a three-fold layer system.
  • the absorption barrier layer system comprises four barrier coating layers, namely a first, a second, a third and a fourth barrier coating layer, counted from the polymeric device to the surface of the barrier system, wherein between the first and the second, between the second and the third, and between the third and the fourth barrier coating layer each a polymeric interface layer is deposited, and wherein the first barrier layer is deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels.
  • Such an absorption barrier layer system is called a four-fold layer system.
  • the at least one polymeric interface layer is located on the surface of the pores, wells and/or microchannels, on a crosslinked superficial section of the device, located between two barrier coating layers, or at least two polymeric interface layers are located on the surface area of the polymeric device, in particular pores, wells and/or microchannels, or the crosslinked superficial section and between two barrier coating layers.
  • the above identified single-layer, double-layer, threefold layer or four-fold layer system is provided, wherein the identified polymeric interface layers and barrier coating layers are deposited not directly on the polymeric device but instead on a cross-linked superficial section thereof, the latter thereby forming also part of the absorption barrier layer system.
  • the absorption barrier layer system of the present invention is transparent.
  • the absorption barrier layer system of the present invention and the top-coating deposited on the surface thereof is transparent.
  • a “hybrid multi-layered absorption barrier layer system” is a system comprising at least one, preferably two, three or four barrier coating layers, and at least one, preferably two or three, polymeric interface layers, and, optionally, a crosslinked superficial section of the device, preferably wherein the at least one interface layer separates the barrier coating layers from each other, separates the surface of the polymeric device, in particular pores, wells and/or microchannels from the barrier coating layer, separates the crosslinked superficial section of the device from the barrier coating layer, or performs two or three of the functions.
  • the at least one polymeric interface layer and the at least one barrier coating layer are deposited on the surface of the polymeric device, in particular pores, wells and/or microchannels, or on the crosslinked superficial section of the device in an alternating manner.
  • a preferred hybrid multi-layered absorption barrier layer system of the present invention comprises at least one preferably rigid, preferably glass-like, for instance Si0 2 -based, barrier coating layer and at least one preferably flexible, preferably polymer-like, for instance polymeric, interface layer.
  • the at least one preferably rigid, preferably glass-like, preferably Si0 2 -based, barrier coating layer is mainly responsible for the barrier effect, inhibiting or at least reducing the partitioning of substances from the sample into the polymeric device.
  • the at least one flexible, preferably polymer-like, preferably polymeric, interface layer improves the barrier characteristics during stretching by presenting an extended tortuous path for the partitioning of the substance.
  • any substance, in particular hydrophobic substance, into the polymeric, preferably PDMS, device will be avoided or significantly reduced.
  • stretching of the polymeric, preferably PDMS, device leads to cracks or gaps in the individual barrier coating layers, whereby, however, due to the multi-layer characteristics and the presence of the polymeric interface layers non-alignment of cracks in the polymeric device and/or the barrier coating layers will lead to an extended diffusion path for the substances and thereby reduce their partitioning from the sample into the substrate.
  • the present invention in particular provides an improved and advantageous coated polymeric device, preferably microfluidic device, all culture device or membrane which avoids or reduces the partitioning of substances from a sample into the polymeric device, e.g. the PDMS substrate, in particular under circumstances, wherein the polymeric device is subjected to external forces causing stretching and/or elongation of the microfluidic device, in particular stretching or bending, preferably up to an elongation of 30 %.
  • the present invention therefore provides an improved coated polymeric device, in particular microfluidic device, cell culture device or membrane which provides a desired flexibility thereby avoiding effects of substance partitioning into the device itself.
  • the at least one barrier coating layer has a thickness of 5 to 500 nm, preferably 10 to 200 nm, preferably 50 to 100 nm, preferably 50 to 180 nm, in particular 10 to 150, in particular 20 to 120 nm, preferably 15 to 50 nm.
  • the polymeric interface layer has a thickness of 5 to 500 nm, preferably 10 to 200 nm, preferably 50 to 100 nm, preferably 50 to 180 nm, in particular 10 to 150, in particular 20 to 120 nm, preferably 15 to 50 nm.
  • the polymeric device is made from a polymer, preferably PDMS, PMMA (polymethylmethylacrylate), PVC (polyvinyl chloride), COC (cyclic olefine copolymer), PS (polystyrene), PC (polycarbonate), polyimide, polyurethane, polyethylene terephthalate (PET), polyesters, in particular polycaprolactone (PCT), or combination of two or more of the polymers.
  • PDMS polymer
  • PMMA polymethylmethylacrylate
  • PVC polyvinyl chloride
  • COC cyclic olefine copolymer
  • PS polystyrene
  • PC polycarbonate
  • polyimide polyurethane
  • PET polyethylene terephthalate
  • PET polycaprolactone
  • the at least one barrier coating layer is a coating layer selected from the group consisting of a Si0 2 -based coating layer, a ZnO-based coating layer, an Al 2 0 3 -based coating layer, a SiC x O y N z -based coating layer (with x, y and z each being independently from 0 to 4, preferably being 0, 1, 2, 3 or 4), a SiC-based coating layer, (a SiOC-based coating layer, a SiON-based coating layer) , a Si 3 N 4 -based coating layer, a Ti0 2 - based coating layer, a metallic-based coating layer, in particular Al-based coating layer, or a ceramic-based coating layer.
  • the at least one barrier coating layer is a SiCxOyN z - based coating layer, in particular a SiCxOyN z coating layer, preferably is consisting of SiCxOyN z (each with x, y and z each being independently from 0 to 4, preferably being 0, 1, 2, 3 or 4).
  • the at least one barrier coating layer is a Si0 2 -based coating layer, in particular a Si0 2 coating layer, preferably is consisting of Si0 2 .
  • the at least one barrier coating layer is a ZnO-based coating layer, in particular a ZnO coating layer, preferably is consisting of ZnO.
  • the at least one barrier coating layer is a A1 2 0 3 - based coating layer, in particular a AI 2 O 3 coating layer, preferably is consisting of AI 2 O 3 .
  • the at least one barrier coating layer is a Ti0 2 - based coating layer, in particular a Ti0 2 coating layer, preferably is consisting of Ti0 2 .
  • the at least one barrier coating layer is a SiC-based coating layer, in particular a SiC coating layer, preferably is consisting of SiC.
  • the at least one barrier coating layer is a SiOC-based coating layer, in particular a SiOC coating layer, preferably is consisting of SiOC.
  • the at least one barrier coating layer is a Si 3 N 4 -based coating layer, in particular a Si 3 N 4 coating layer, preferably is consisting of Si 3 N 4 .
  • the at least one barrier coating layer is a SiON-based coating layer, in particular a SiON coating layer, preferably is consisting of SiON.
  • the at least one barrier coating layer is a metallic-based layer, preferably Al.
  • the at least one barrier coating layer is a ceramic-based coating layer, in particular a ceramic coating layer, preferably is consisting of ceramics.
  • Thicknesses of the coating and interface layers can be controlled by ellipsometry and scanning electromicroscopy (SEM) measurements.
  • the at least one polymeric interface layer and the at least one barrier coating layer are deposited on each other essentially coextensively or completely coextensively.
  • the at least one polymeric interface layer and the at least one barrier coating layer are arranged coextensively to each other and are preferably arranged essentially or completely coextensively on an optionally present crosslinked superficial section of the polymeric device.
  • the surface of the polymeric device in particular of the pores, wells and/or microchannels, is at least partially, preferably completely covered by an absorption barrier layer system.
  • an absorption barrier layer system preferably at least a part, preferably the complete, wall of the pores, wells and/or microchannels, is covered by the absorption barrier layer system.
  • the surface of the absorption barrier layer system which is preferably formed from the most outwardly located barrier coating layer, is covered with a top coating.
  • a top coating can, preferably, have a thickness from 5 to 500 nm, preferably 50-300 nm, preferably 20 to 100 nm.
  • such a top coating is a functional coating of the surface of the absorption barrier layer system, for instance rendering said surface hydrophobic, hydrophilic, aerophob or aerophil.
  • chemical groups such as amino groups, carboxyl groups, thiol groups, phosphor groups or other organyl groups may be linked to the surface such as the provide the top coating.
  • the top coating can comprise metal or metal oxide layers such as A1-, Zn-, ZnO-, AI 2 O 3 -, Si0 2 - or Ti0 2 -layers.
  • the top coating may have surface energy from 10 to 180 mJ/m 2 and/or have a surface roughness (Ra) of 0.2 to 50 nm, preferably 1 to 10 nm.
  • the absorption barrier layer system as disclosed herein consists of the layers and elements being individually and explicitly disclosed herein.
  • the absorption barrier layer system of the coated polymeric device of the present invention includes no other material layers or elements than those which are explicitly disclosed herein, namely the at least one polymeric interface layer, the at least one barrier coating, and, optionally, the crosslinked superficial section of the substrate.
  • the present invention also provides a process for preparing a coated polymeric device, in particular a microfluidic device, coated cell culture device, coated membrane according to the present invention comprising a) providing a polymeric device, preferably a PDMS device, in particular a polymeric device, preferably a PDMS device, comprising a system of microchannels, preferably open microchannels, a system of pores and/or a system of wells in the polymeric device, b) subjecting the polymeric device, preferably the PDMS device, to at least one CVD process, in particular at least one PECVD process, so as to provide an absorption barrier layer system comprising at least one polymeric interface layer and at least one barrier coating layer and c) obtaining the coated polymeric device, in particular the coated microfluidic device, coated cell culture device and/or coated membrane, according to the present invention.
  • the polymeric device, in particular microfluidic device, provided in step a) comprising a system of open microchannels is a precursor for obtaining a fully-assembled polymeric device, in particular microfluidic device
  • the present invention requires assembling the polymeric device, in particular microfluidic device, from the precursor polymeric device, in particular microfluidic device, prepared according to the present invention and either another precursor polymeric device, in particular microfluidic device, according to the present invention or another precursor to prepare the fully-assembled polymeric device, in particular microfluidic device.
  • step a) after process step a) and prior to step b), the polymeric substrate of step a) is subjected to a further process step axb) which is a crosslinking process so as to provide a crosslinked superficial section of the polymeric device.
  • a process for preparing a coated polymeric device comprising a) providing a polymeric device, preferably a PDMS device, in particular a polymeric device, preferably a PDMS device, comprising a system of microchannels, preferably open microchannels, a system of pores and/or a system of wells in the polymeric device, axb) subjecting the polymeric device of step a) to a crosslinking process, so as to provide a crosslinked superficial section of the polymeric device, b) subjecting the crosslinked superficial section of the polymeric device obtained in step axb) to at least one CVD process, in particular at least one PECVD process, so as to provide an absorption barrier layer system comprising at least one polymeric interface layer and at least one barrier layer on the crosslinked superficial section of the polymeric device and c) obtaining the coated polymeric device,
  • the polymeric device preferably PDMS device
  • a crosslinking process so as to provide an absorption barrier layer system comprising a crosslinked superficial section of the polymeric device, preferably the PDMS device, on which, in a preferred embodiment, one or more barrier coating layers and polymeric interface layers are deposited.
  • the CVD process, in particular PECVD process, of step b) is repeated 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more times, so as to provide one or more barrier coating layers and in addition one or more polymeric interface layers, wherein the polymeric interface layers are deposited on the polymeric device and/or between, that means intervening, two adjacent barrier coating layers, and/or on the crosslinked superficial section of the device.
  • the barrier coating layer preferably the Si0 2 -based coating layer
  • the barrier coating layer is deposited on the polymeric device by a chemical vapour deposition process (CVD-process), preferably a PECVD process (plasma enhanced chemical vapour deposition), preferably using tetraethyl orthosilicate and 0 2 or using silanes, preferably siloxanes, preferably HMDSN or HMDSO, each of them preferably together with N, NH 3 , N 2 0 and/or 0 2 .
  • CVD-process chemical vapour deposition process
  • PECVD process plasma enhanced chemical vapour deposition
  • silanes preferably siloxanes, preferably HMDSN or HMDSO, each of them preferably together with N, NH 3 , N 2 0 and/or 0 2 .
  • SiO x N y , Si 3 N 4 or SiC x O y N z (each with x, y, z each being independently from 0 to 4, preferably being 0, 1 , 2, 3 or 4) can be prepared by a CVD-process, in particular a PECVD process, using HMDSN (hexamethyldisilazane) optionally, using N, NH 3 , N 2 0 and/or 0 2 -carrier gas.
  • HMDSN hexamethyldisilazane
  • the absorption barrier layer system of the present invention comprises at least two barrier coating layers
  • these at least two barrier coating layers are made from different materials. The embodiment, thus, provides barrier coating layers with different degrees of hardness.
  • the polymeric interface layer is deposited by a CVD-process, in particular a PECVD process, preferably using tetraethyl orthosilicate and 0 2 or using silanes, preferably siloxanes, preferably using HMDSO, or parylene.
  • a CVD-process in particular a PECVD process, preferably using tetraethyl orthosilicate and 0 2 or using silanes, preferably siloxanes, preferably using HMDSO, or parylene.
  • the polymeric interface layer is made from plasma polymerized HMDSO, in particular is a polymer layer.
  • the HMDSO-layer consists preferably of 45 to 55 at%, preferably 50 at% C, 20 to 30 at%, preferably 25 at% O and 20 to 30 at%, preferably 25 at% Si.
  • the CVD process for applying the barrier coating and the interface layer is a PECVD process.
  • the PECVD process uses a low pressure plasma method, preferably using a pressure ⁇ 1 mbar, and preferably employs the following general conditions:
  • the layers of the absorption barrier layer system are prepared on the polymeric device in a preferred embodiment by means of a low-pressure plasma method, preferably wherein a plasma is generated from reactive gas by means of a high-frequency discharge between at least two electrodes, which gas contains a suitable starting molecule, for instance HMDSO.
  • the gas atmosphere has a low pressure, for example a pressure ⁇ 1 mbar, and/or uses process gases for instance 0 2 , He, Ar or N 2 as well as corresponding starting polymers for the production of the coatings.
  • HMDSO is preferably used as starting molecule.
  • the gas molecules are ionized, fragmented and activated by means of igniting a high-frequency plasma for example at 13,56 MHZ between two electrodes so that a plasma is formed.
  • Chemical reaction can take place in the plasma phase or on the surface to be coated which reactions lead to covalent binding of the plasma polymer products on the surface to be coated.
  • the at least one barrier coating layer has been obtained by atomic layer deposition.
  • the crosslinking for providing the crosslinked superficial section of the polymeric device may be conducted in a plasma, preferably in a low pressure plasma, that means is a plasma-based, in particular low pressure plasma-based method, for instance using a pressure ⁇ 1 mbar, by UV and/or VUV radiation, for instance in a range from 150 to 350 nm.
  • a plasma-based, in particular low pressure plasma-based method for instance using a pressure ⁇ 1 mbar
  • UV and/or VUV radiation for instance in a range from 150 to 350 nm.
  • 0 2 - plasmas can be used, or He, N 2 , N0 2 , NO, C0 2 , or Ar.
  • the plasma-based method of crosslinking is conducted using an area of the electrode having a format of DIN A3 and, preferably a power of 50 to 800 W, preferably of 100 W, RF (radio frequency) at 13,56 MHz.
  • the frequency used can be from preferably 3kHz to 150 kHz, or be 2.4 GHz.
  • the 0 2 , He, N 2 , N0 2 , NO, C0 2 , or Ar gas flow is from 100 to 300 seem, preferably 200 seem.
  • the pressure is a low pressure, in particular from 0.05 to 1.5 mbar, preferably 0.11 mbar.
  • the treatment time for the plasma-based crosslinking method step is from 5 s to 15 min, preferably 5 s to 60 s, preferably 1 min to 10 min.
  • the UV-/VUV-radiation of the polymeric device for crosslinking can be done by using UV radiation from excimer lamps like Xe 2 * (wavelength at 172nm), XeBr* (wavelength at 282 nm), KrCl* (wavelength at 222 nm), or XeCl* (wavelength at 308 nm), or by using the irradiation from a mercury vapor lamp (wavelength at 254 nm) or using deep UV (DUV) LEDs at wavelength of 265 nm, 280 nm, 285 nm or 300 nm, in general using DUV LEDs ranging from 265 nm - 365 nm, e.g. of Nikkiso.
  • the irradiation time is approx. 1 - 10 min in dependence of the radiation intensity (in mW/cm 2 ) and the distance of the external irradiation source (lamp).
  • the present processes provide surfaces of coated polymeric devices, in particular microfluidic devices, cell culture devices or membranes which are very smooth.
  • the present processes also allow the preparation of individual polymeric and barrier mono-layers up to the thickness of only 3 ⁇ . Thus, even very small and narrow pores, wells and/or microchannels having diameters of only one or several ⁇ can be coated with the present process.
  • the present invention also relates to coated polymeric devices, in particular micro fluidic devices, cell culture devices or membranes obtainable according to the present process.
  • the coated polymeric devices in particular microfluidic device, cell culture devices or membranes as disclosed or as prepared by the present invention is preferably a flexible and biocompatible device and preferably can be used for numerous applications. For instance, it can be used for the preparation of a microfluidic chip, organ-on-a-chip, as a DNA- or RNA-chip, as an inkjet-head, or can be used as lab-on-a-chip.
  • coated polymeric device in particular microfluidic devices, cell culture devices or membranes may also be used for the screening, development or analysis of potential or established drugs, for medical, clinical and forensic analysis, 3D-printing, biological and chemical analysis, environmental monitoring or molecular diagnostic.
  • a "plasma” is a partially or completely ionised gas and/or vapour, which in addition to ions and electrons contains chemical radicals and a number of electronical exited particles.
  • a microfluidic device is a device comprising in a three- dimensional polymeric substrate, that means on the surface of the substrate or embedded in the substrate, a system of microchannels, in particular at least one microchannel, through which a fluid can pass, preferably several microchannels, preferably connected microchannel, preferably a network of connected microchannels, wherein the at least one microchannel has at least one internal cross-sectional dimension (i.e.
  • depth, width or length that is less than 1000 ⁇ , in particular has at least one diameter in the diameter range from 0.1 to 990, preferably 0.5 to 900 ⁇ , in particular 0.5 to 800 ⁇ , preferably 1 to 400 ⁇ , preferably 1 to 10 ⁇ , preferably 1 to 5 ⁇ .
  • a "system of microchannels" herein is also termed "microchannels”.
  • an "open microchannel” is a microchannel, the wall of which does not completely enclose the void of the microchannel along its longitudinal axis and which is being provided on the surface of a microfluidic device or on the surface of a precursor for a micro fluidic device, for instance a polymeric sheet prior to bonding, that means assembling, of a microfluidic device.
  • a microchannel can be an open microchannel or a microchannel being embedded in a polymeric substrate.
  • a microfluidic device comprising a system of embedded microchannels according to the present invention is obtainable from a precursor microfluidic device comprising a system of open microchannels according to the present invention and being obtainable by a process of the present invention.
  • a system of pores herein also termed “pores”, means at least one pore, preferably 2, 3, 4 or more pores, in particular a multitude of pores.
  • a system of wells herein also termed “wells”, means at least one well, in particular 1, 2, 3, 4, or more wells, preferably a multitude of wells.
  • the surface of a pore, well or microchannel is a wall of the pore, well or microchannel.
  • a schematic drawing (top view) of a micro fluidic device comprising a system of microchannels in the polymeric device.
  • a schematic drawing (cross sectional view) of another microfluidic device of the present invention is shown.
  • FIG. 6A to 6D Schematic drawings of a single-layer (6A), double-layer (6B), three-fold-layer (6C) and fourfold (6D) layer absorption barrier layer system of the present invention with a top coating, each on a PDMS device.
  • Figure 7 A schematic drawing showing the tortuous path of a permeating substance through a hybrid multilayered barrier system of the present invention.
  • a light-microscopic (9 A) and fluorescence microscopic (9B) image (cross-section) of a coated polymeric device comprising a double-layer absorption barrier layer system of the present invention are shown in FIG. 1 .
  • Figure 10 shows graphically the fluorescence intensity (A.U., arbitrary units) in dependency upon the penetration depth, measured in untreated polymeric devices and polymeric devices comprising an absorption barrier layer system of the present invention after rhodamine 6G application.
  • Figure 11 shows graphically the fluorescence intensity (A.U., arbitrary units) in dependency upon the penetration depth, measured in untreated polymeric devices and polymeric devices comprising an absorption barrier layer system of the present invention after rhodamine 101 application.
  • Figure 12 shows schematically a polymeric device (10) of the present invention comprising wells and pores.
  • Example 1 Structures of microfluidic devices of the present invention
  • Figure 1 shows a microfluidic device of the present invention in top view comprising a polymeric device (10), made from PDMS, and a system (20) of microchannels (25).
  • Figure 2 shows in cross-sectional view a microfluidic device with a microchannel (25) located on the surface (26) of a polymeric device (10).
  • Figure 3 shows, in cross-sectional view, a microfluidic device and a microchannel (25), wherein the surface of the microchannel (25) is formed by an absorption barrier layer system (30) which comprises a polymeric interface layer (40) made by a PECVD process from HMDSO and which is located on the surface of the microchannel (25) and on which a barrier coating layer (50), which is an Si0 2 -layer made by a PECVD process using HMDSO and 0 2 , is deposited thereon, thereby forming the surface of the microchannel (25).
  • the absorption layer system (30) comprises a polymeric-interface layer (40) deposited on the surface of the microchannel (25) and a barrier coating layer (50) deposited on the polymeric interface layer (40).
  • Figure 4 shows essentially the same embodiment as given in Figure 3, except that the polymeric interface layer (40) is deposited on a cross-linked superficial section (60) of the surface of the microchannel (25).
  • the absorption barrier layer system comprises the cross- linked superficial section (60) made in a plasma process using UV radiation and on which a polymeric interface layer (40) is deposited and on which polymeric interface layer (40), a barrier coating layer (50) is deposited thereon, thereby forming the most outwardly located surface coating of the microchannel (25).
  • Figure 5A shows schematically the structure of a single-layer absorption barrier layer system (30) of the present invention, comprising a polymeric interface layer (40) deposited on the surface of the substrate (10) or on a non-depicted cross-linked superficial section of the device, and on top of the polymeric interface layer (40) a barrier coating layer (50).
  • Figure 5B shows an absorption barrier layer system (30) which is a double-layer system comprising on the surface of the device (10) a first barrier coating layer (51), a polymeric interface layer (40) and a second barrier coating layer (52).
  • Figure 5C shows an absorption barrier layer system (30), namely a three-fold layer system (30) located on the surface of a microchannel, i.e. on the polymeric device (10), which system comprises a first barrier coating layer (51), a first polymeric interface layer (41), a second barrier coating layer (52), a second polymeric interface layer (42), and a third barrier coating layer (53).
  • Figure 5D shows an absorption barrier layer system (30), namely a four-fold layer system (30) located on the device (10) which comprises a first barrier coating layer (51), a first polymeric interface layer (41), a second barrier coating layer (52), a second polymeric interface layer (42), a third barrier coating layer (53), a third polymeric interface layer (44) and a fourth barrier coating layer (54).
  • Figures 6 A, 6B, 6C and 6D show absorption barrier layer systems (30) as depicted in Figures 5 A to 5D except that on each of the most outwardly located surface of said absorption barrier layer systems (30) a top coating (80) is located.
  • FIG. 7 shows a cross-sectional view of an absorption barrier layer system (30) provided on a polymeric device (10).
  • the absorption barrier layer system comprises a first barrier coating layer (51) deposited on the polymeric substrate (10) forming a surface of the microchannel (25), a first polymeric interface layer (41), a second barrier coating layer (52) deposited on the first polymeric interface layer (41), a second polymeric interface layer (42) deposited on the second barrier coating layer (52), a third barrier coating layer (53) deposited on the second polymeric interface layer (42), a third polymeric interface layer (43) deposited on the third barrier coating layer (53) and a fourth barrier coating layer (54) deposited on the third polymeric interface layer (43).
  • the barrier coating layers are rigid barrier coating layers, in particular Si0 2 -coating layers, while the polymeric interface layers are polymer-like flexible layers.
  • the gaps (75) formed in the different barrier coating layers (51, 52, 53,54) are spatially separated from each other by the more flexible polymeric interface layers (41, 42, 43), thereby leading to an extended torturous pathway (arrow) for any penetrating substance entering the surface of the microchannel towards the direction of the substrate.
  • an extended pathway for substances stemming from the sample into the substrate is provided, reducing or avoiding any absorption of a substance present in the sample in the substrate itself.
  • Figure 12 shows schematically a polymeric device in the form of a cell culture device (10) comprising a system of pores (110) and a system of wells (120).
  • the complete surface of the device (10) including the walls of the system of pores (110) and of the system of wells (120) is covered with an absorption barrier layer system (30).
  • master-wafers are fabricated via standard photolithography. Briefly, layers of photoresist (SU-8 or similar) with defined thickness are obtained via spin coating the resist onto silicon wafers. The photoresist is exposed to UV light through a mask with the desired lateral structures. Before and after exposure to UV light the wafers are placed on hot plates and heated up to defined temperatures. Subsequently, the non- exposed/non-crosslinked areas of the photoresist are removed by immersion of the patterned wafers in photoresist developer. Following a final baking step, the patterned surface is coated with a fluorosilane.
  • photoresist SU-8 or similar
  • the microfluidic PDMS devices are replica molded by pouring uncured PDMS with application-specific ratio of a curing agent to a prepolymer onto the master-wafers, which are subsequently cured at elevated (50-90°C) temperatures for multiple (2-10) hours.
  • the final PDMS devices are obtained by peeling the cured molds from the wafer and cutting them into individual modules, in which access holes are punched. The modules are then bonded to glass slides via exposure to oxygen plasma, followed by a baking step.
  • the absorption barrier layer system is applied in a reactor of an area of DNA3 using the following process parameters.
  • All plasma processes in this example are radio frequency (RF) plasmas at 13.56 MHz.
  • the optionally present crosslinked superficial section of the polymer device is prepared using the following process parameters of a plasma-based process:
  • Treatment time lO min.
  • the irradiation of the polymeric device for crosslinking is carried out using UV radiation from excimer lamps like Xe 2 * (wavelength at 172nm), XeBr* (wavelength at 282 nm), KrCl* (wavelength at 222 nm), or XeCl* (wavelength at 308 nm), or by using the irradiation from a mercury vapor lamp (wavelength at 254 nm) or using deep UV (DUV) LEDs at wavelength of 265 nm, 280 nm, 285 nm or 300 nm (in general using DUV LEDs ranging from 265 nm - 365 nm) (Nikkiso).
  • the irradiation time is approx. 1 - 10 min in dependence of the radiation intensity.
  • Penetration analysis was performed on PDMS devices in form of slabs which have been prepared using a 1 : 10 w/w ratio of curing agent to prepolymer that were cured for 4 hours at 70°C in a convection oven.
  • Non-treated, i.e. non-coated, PDMS devices and coated PDMS devices of the present invention comprising an absorption barrier layer system were incubated in 600 ⁇ of Rhodamine 6G and Rhodamine 101 solutions at different concentrations. After 60 min incubation in physiological environment, the Rhodamine solutions were aspirated and the samples rinsed in i) 600 ⁇ 1 PBS, ii) 1200 ⁇ 1 PBS and iii) 60 ⁇ 1 DI Water. Multiple cross-sections were prepared by cutting the samples using a razor blade. The cross-sections were then imaged using a fluorescence microscope maintaining the exact same settings for all cross-sections and samples. Average fluorescence intensity in dependency upon the penetration depths was analysed using ImageJ "plot profile" tool.
  • Figure 8 A shows a light-microscopic image of a cross-section through a non-treated, that means non-coated, PDMS device having no crosslinked superficial section, which has been incubated for 60 min with rhodamine 6G solution prior to the analysis.
  • Figure 8B shows a fluorescence microscopic image thereof, clearly indicating the significant penetration of the rhodamine 6G solution into the device.
  • Figure 9 A shows a light-microscopic image of a cross-section of a micro fluidic device according to the present invention, comprising a double-layer absorption barrier layer system, which comprises a first barrier coating layer on the polymeric device, a polymeric interface layer deposited thereon and a second barrier coating layer coated thereon, which has been incubated for 60 min with rhodamine 6G solution prior to the analysis.
  • a double-layer absorption barrier layer system which comprises a first barrier coating layer on the polymeric device, a polymeric interface layer deposited thereon and a second barrier coating layer coated thereon, which has been incubated for 60 min with rhodamine 6G solution prior to the analysis.
  • Figure 9B shows a fluorescence microscopic image thereof, clearly indicating that even after 60 minutes of rhodamine 6G incubation no permeation of the solution into the polymeric device occurred.
  • Figure 10 shows the fluorescence intensity of rhodamine 6G in dependency upon the penetration depths in the polymeric device for two different concentrations of rhodamine 6G (0,05 and 0,75 mg/ml) on a non-treated, i.e. non-coated polymeric device having no crosslinked superficial section according to figure 8 and on a coated polymeric device of the present invention comprising an absorption barrier layer system according to figures 5B and 9 (first barrier layer: Si0 2 , polymeric interface layer, second barrier coating layer: Si0 2 ). It is clearly evident that there is no penetration into the polymeric device being covered with the absorption barrier system of the present invention, while in non-treated substrates a significant penetration into the polymeric device occurred.
  • first barrier layer Si0 2
  • second barrier coating layer Si0 2
  • Figure 11 shows the fluorescence intensity of rhodamine 101 in dependency upon the penetration depths in the polymeric device for two different concentrations of rhodamine 101 (10 mM, 100 mM) on a non-treated, i.e. non-coated polymeric device having no crosslinked superficial section according to figure 8 and on a coated polymeric device of the present invention comprising an absorption barrier layer system according to figures 5B and 9 (first barrier layer: Si0 2 , polymeric interface layer, second barrier coating layer: Si0 2 ). It is clearly evident that there is no penetration into the polymeric device being covered with the absorption barrier system of the present invention, while in non-treated polymeric devices a significant penetration into the polymeric device occurred.

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Abstract

Un dispositif polymère, en particulier un dispositif microfluidique (100), un système de culture cellulaire ou une membrane, comprend un système (20) de microcanaux (25), de puits et/ou de pores dans le dispositif polymère (10), au moins une partie de la surface des microcanaux (25), des puits ou des pores est formée par un système de couche barrière d'absorption, le système de couche barrière d'absorption comprenant au moins une couche d'interface polymère (40) et au moins une couche de revêtement barrière (50).
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