EP3583213A4 - Single sperm gene expression and mutation analysis for prediction of diseases - Google Patents

Single sperm gene expression and mutation analysis for prediction of diseases Download PDF

Info

Publication number
EP3583213A4
EP3583213A4 EP18754777.3A EP18754777A EP3583213A4 EP 3583213 A4 EP3583213 A4 EP 3583213A4 EP 18754777 A EP18754777 A EP 18754777A EP 3583213 A4 EP3583213 A4 EP 3583213A4
Authority
EP
European Patent Office
Prior art keywords
prediction
diseases
gene expression
mutation analysis
single sperm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18754777.3A
Other languages
German (de)
French (fr)
Other versions
EP3583213A1 (en
EP3583213B1 (en
Inventor
Christopher E. MASON
Jeffrey R. ROSENFELD
Delia TOMOIAGA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Cornell University
Original Assignee
Rutgers State University of New Jersey
Cornell University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey, Cornell University filed Critical Rutgers State University of New Jersey
Publication of EP3583213A1 publication Critical patent/EP3583213A1/en
Publication of EP3583213A4 publication Critical patent/EP3583213A4/en
Application granted granted Critical
Publication of EP3583213B1 publication Critical patent/EP3583213B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/20—Sequence assembly
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/30—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indices; for individual health risk assessment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Public Health (AREA)
  • Evolutionary Biology (AREA)
  • Databases & Information Systems (AREA)
  • Primary Health Care (AREA)
  • Epidemiology (AREA)
  • Data Mining & Analysis (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP18754777.3A 2017-02-17 2018-02-17 Single sperm gene expression and mutation analysis for prediction of diseases Active EP3583213B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762460408P 2017-02-17 2017-02-17
PCT/US2018/018534 WO2018152432A1 (en) 2017-02-17 2018-02-17 Single sperm gene expression and mutation analysis for prediction of diseases

Publications (3)

Publication Number Publication Date
EP3583213A1 EP3583213A1 (en) 2019-12-25
EP3583213A4 true EP3583213A4 (en) 2020-12-02
EP3583213B1 EP3583213B1 (en) 2023-04-19

Family

ID=63169660

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18754777.3A Active EP3583213B1 (en) 2017-02-17 2018-02-17 Single sperm gene expression and mutation analysis for prediction of diseases

Country Status (3)

Country Link
US (1) US12065700B2 (en)
EP (1) EP3583213B1 (en)
WO (1) WO2018152432A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111466338B (en) * 2019-01-23 2022-03-22 中国科学院广州生物医药与健康研究院 Ddx5 gene-deleted spermatogenesis dysfunction mouse model and construction method thereof
CN111321225B (en) * 2020-03-02 2021-08-17 中山大学孙逸仙纪念医院 Application of lncRNA evaluation system in prediction of tumor immunotherapy effect
CN111973744B (en) * 2020-07-15 2022-07-01 北京大学深圳医院 Application of PLCE1-AS2 in breast cancer
CN113436681B (en) * 2021-07-05 2022-02-25 温州谱希医学检验实验室有限公司 Method and related equipment for performing associated statistical test on low-frequency variation and target disease
CN114941028B (en) * 2021-07-16 2023-06-02 武汉艾米森生命科技有限公司 Reagent and kit for detecting and diagnosing colorectal cancer
CN114606311B (en) * 2022-04-11 2023-09-26 河北省生殖健康科学技术研究院 Application of SLC39A13 gene rs755555 locus, detection primer and probe combination thereof and kit
CN115927455A (en) * 2022-06-22 2023-04-07 华中科技大学 Construction method and application of animal model of Bag5 knockout mouse
CN115976033B (en) * 2022-12-14 2024-05-17 西南大学 Melon fly TSSK and TSSK genes and application thereof
CN117025758B (en) * 2023-09-19 2024-04-05 华北理工大学 Application of circular RNA in preparation of biomarker for diagnosing autism

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2031071A1 (en) 2007-08-31 2009-03-04 Minitüb Abfüll- und Labortechnik GmbH & Co. KG Method for categorizing samples containing spermatozoa by molecular profiling
WO2012078602A2 (en) 2010-12-06 2012-06-14 Children's Medical Center Corporation Method for diagnosis and method of treatment of autism spectrum disorders and intellectual disability
CN102952855B (en) * 2011-08-26 2015-05-20 深圳华大基因科技服务有限公司 Genetic map construction method and device, haplotype analytical method and device

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DELIA TOMOIAGA ET AL: "Single-cell sperm transcriptomes and variants from fathers of children with and without autism spectrum disorder", NPJ GENOMIC MEDICINE, vol. 5, no. 1, 21 February 2020 (2020-02-21), XP055741129, DOI: 10.1038/s41525-020-0117-4 *
JIAHUAN CHEN ET AL: "Single-cell SNP analyses and interpretations based on RNA-Seq data for colon cancer research", SCIENTIFIC REPORTS, vol. 6, no. 1, 28 September 2016 (2016-09-28), XP055742021, DOI: 10.1038/srep34420 *
MANDY G. KATZ-JAFFE ET AL: "Aging sperm negatively impacts in vivo and in vitro reproduction: a longitudinal murine study", FERTILITY AND STERILITY, vol. 100, no. 1, 1 July 2013 (2013-07-01), NL, pages 262 - 268.e2, XP055742158, ISSN: 0015-0282, DOI: 10.1016/j.fertnstert.2013.03.021 *
RAKESH SHARMA ET AL: "Effects of increased paternal age on sperm quality, reproductive outcome and associated epigenetic risks to offspring", REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, BIOMED CENTRAL LTD, GB, vol. 13, no. 1, 19 April 2015 (2015-04-19), pages 35, XP021223071, ISSN: 1477-7827, DOI: 10.1186/S12958-015-0028-X *
See also references of WO2018152432A1 *
SHIHONG MAO ET AL: "A comparison of sperm RNA-seq methods", SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, vol. 60, no. 5, 31 July 2014 (2014-07-31), US, pages 308 - 315, XP055741310, ISSN: 1939-6368, DOI: 10.3109/19396368.2014.944318 *
SPIESS A -N ET AL: "Gene expression analysis of single human spermatozoa: Approaches and caveats", JOURNAL FUR REPRODUKTIONSMEDIZIN UND ENDOKRINOLOGIE 2017 KRAUSE UND PACHERNEGG GMBH NLD, vol. 14, no. 5, 2017, pages - 7. DVR, XP009523545, ISSN: 1810-9292 *
TIAN YU ET AL: "Microfluidics-based digital quantitative PCR for single-cell small RNA quantification+", BIOLOGY OF REPRODUCTION, vol. 97, no. 3, 31 August 2017 (2017-08-31), US, pages 490 - 496, XP055591868, ISSN: 0006-3363, DOI: 10.1093/biolre/iox102 *

Also Published As

Publication number Publication date
US20200010896A1 (en) 2020-01-09
EP3583213A1 (en) 2019-12-25
EP3583213B1 (en) 2023-04-19
US12065700B2 (en) 2024-08-20
WO2018152432A1 (en) 2018-08-23

Similar Documents

Publication Publication Date Title
EP3583213A4 (en) Single sperm gene expression and mutation analysis for prediction of diseases
EP3662066A4 (en) Cellular models of and therapies for ocular diseases
EP3458585A4 (en) Gene therapy methods for age-related diseases and conditions
EP3402885A4 (en) Chimeric proteins and methods of regulating gene expression
IL261882A (en) Genetic variant-phenotype analysis system and methods of use
EP3548652A4 (en) Methods of assaying proteins
EP3289080A4 (en) Gene therapy for autosomal dominant diseases
HUE056228T2 (en) Computer classification of biological tissue
EP3452166A4 (en) Intranasal stimulation for treatment of meibomian gland disease and blepharitis
EP3350313A4 (en) Derivation of liver organoids from human pluripotent stem cells
EP3365037A4 (en) Derivation of human skin organoids from pluripotent stem cells
EP3369221A4 (en) Rapid predictive analysis of very large data sets using the distributed computational graph
EP3824080A4 (en) Methods of analyzing cells
EP3320469A4 (en) Genetic and genealogical analysis for identification of birth location and surname information
EP3188667A4 (en) Gene analysis and generation of stem cell methods and apparatus
EP3237004A4 (en) Predicting outcome of treatment with an anti- 4 7 integrin antibody
EP3322815A4 (en) Compositions and methods for detection of genetic deafness gene mutation
EP3308310A4 (en) Systems and methods for patient-specific prediction of drug responses from cell line genomics
EP3721377A4 (en) Systems and methods of efficiently performing biological assays
EP3445876A4 (en) Single cell analysis of transposase accessible chromatin
IL265850A (en) Humanized anti-muc1 antibodies and use of cleavage enzyme
EP3481853A4 (en) Genetic perturbation of the rna degradosome protein complex
EP3448985A4 (en) Differentiation of cortical neurons from human pluripotent stem cells
EP3732689A4 (en) Methods of performing clinical trials
EP3294911A4 (en) Platform for discovery and analysis of therapeutic agents

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20190816

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20201029

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/10 20060101ALI20201023BHEP

Ipc: C12Q 1/6883 20180101AFI20201023BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20211126

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602018048609

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: C12N0015100000

Ipc: C12Q0001688300

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6883 20180101AFI20220930BHEP

INTG Intention to grant announced

Effective date: 20221028

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602018048609

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 1561226

Country of ref document: AT

Kind code of ref document: T

Effective date: 20230515

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230606

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG9D

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20230419

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 1561226

Country of ref document: AT

Kind code of ref document: T

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230821

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230719

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230819

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230720

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602018048609

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20240122

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20240228

Year of fee payment: 7

Ref country code: CH

Payment date: 20240301

Year of fee payment: 7

Ref country code: GB

Payment date: 20240227

Year of fee payment: 7

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20240226

Year of fee payment: 7

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230419

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20240217