EP3522738A1 - Food product or beverage containing a marine microalga - Google Patents

Food product or beverage containing a marine microalga

Info

Publication number
EP3522738A1
EP3522738A1 EP17790968.6A EP17790968A EP3522738A1 EP 3522738 A1 EP3522738 A1 EP 3522738A1 EP 17790968 A EP17790968 A EP 17790968A EP 3522738 A1 EP3522738 A1 EP 3522738A1
Authority
EP
European Patent Office
Prior art keywords
odontella
product according
extract
biomass
product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17790968.6A
Other languages
German (de)
French (fr)
Inventor
Pierre Calleja
Alain Guillou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Odontella
Original Assignee
Odontella
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Odontella filed Critical Odontella
Publication of EP3522738A1 publication Critical patent/EP3522738A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A61K36/03—Phaeophycota or phaeophyta (brown algae), e.g. Fucus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to food products and beverages comprising a marine microalgae.
  • the invention also relates to the use of this marine microalgae as a booster and / or flavor enhancer in foods or beverages.
  • Microalgae are freshwater or marine aquatic organisms with a high concentration of high quality nutrients, including whole proteins, polyunsaturated fatty acids, fiber and polysaccharides, minerals, water-soluble vitamins, pigments and antioxidants. In addition, they are first-tier organisms that accumulate fewer pollutants and harmful compounds. In addition, the production of microalgae is by culture which does not involve harvesting in the natural environment unlike obtaining other aquatic organisms, and thus allows better control of the quality and traceability of finished products.
  • these microorganisms do not possess certain molecules that are essential for health, in particular long-chain polyunsaturated fatty acids from the omega 3 family that are today provided by the use of animal products (fish oil, krill, shrimps or molluscs especially) so not accessible to a growing part of the population (allergic, vegetarians, vegans, conscientious ecologists, etc.). In addition, these microorganisms do not have any taste interest in food products or beverages.
  • the objective of the invention is to propose products intended for feeding on the basis of a raw material of algal origin, the use of which makes it possible at the same time to increase the levels of EPA and DHA, fucoxanthin and other pigments.
  • antioxidants in food products without using products of animal origin provide organic silica and enhance and / or improve the taste of these products by limiting the amount of sodium they contain.
  • the invention relates to the use of a marine microalgae and extract of a microalga Odontella genus in food products.
  • their use according to the invention makes it possible to increase the content of EPA and DHA in food products, as well as other molecules such as fucoxanthin, carotenoids with high oxidizing power and organic silica that integrates with the bone matrix and allows for more hard and resistant bones.
  • Odontella in food products enhances the taste and brings new flavors that enhance the flavors and tastes of the sea and increase the organoleptic quality of the product.
  • the subject of the invention is a product intended for human or animal consumption, in the form of food or drink, comprising between 0.2 and 50% by weight of biomass of a marine microalgae of the genus Odontella and or at least one extract of said microalgae.
  • the invention also relates to the use of biomass of a marine microalgae of the genus Odontella and / or at least one extract of said microalga as enhancer and / or flavor enhancer in food products or beverages.
  • biomass of a marine microalgae of the genus Odontella within the meaning of the invention is meant the entire cellular material.
  • extract within the meaning of the invention is meant any mixture of several native molecules contained in the Odontella biomass, obtained by one or more extraction methods.
  • booster and / or flavor enhancer within the meaning of the invention means a taste and typically marine flavors and / or a more pronounced taste of marine aroma that increases the organoleptic qualities of the product.
  • the subject of the invention is a product intended for human or animal consumption, in the form of food or drink, comprising between 0.2 and 50% by weight of a marine microalgae of the genus Odontella.
  • the product intended for consumption according to the invention can be in particular:
  • a veterinary food or "pet food” intended for animal consumption for example a specific feed for particular growth phases of the animal (nursery stage, reproduction, prevention of diseases growth etc.) ...) ..
  • a veterinary drink intended for animal consumption for example a dietary supplement specific to particular growth stages of the animal (nursery stage, reproduction, prevention of growth diseases, etc.),
  • a food intended for human consumption such as a cereal product, a bakery product, a sweet or savory cake, a sweet or savory biscuit, a candy, a soup, a vegetable crisps, a condiment, an oil, a cooked dish, a product processed from marine products (terrine, surimis, etc.), a dairy product or a liquid derived from plants fermented or not,
  • a drink for human consumption such as a soda, a fruit juice, an energy drink, flavored water, a syrup or an alcoholic beverage.
  • the product according to the invention contains between 0.2 and 50% by weight:
  • At least one extract of a marine microalgae of the genus Odontella is at least one extract of a marine microalgae of the genus Odontella.
  • the marine microalgae (s) present in the products according to the invention may be of any species belonging to the genus Odontella, such as Odontella aurita, Odontella granulata, Odontella longicruris, Odontella mobiliensis, Odontella regulated Odontella rhombus, Odontella simensis.
  • Odontella aurita is an species belonging to the genus Odontella, such as Odontella aurita, Odontella granulata, Odontella longicruris, Odontella mobiliensis, Odontella regulated Odontella rhombus, Odontella simensis.
  • Odontella aurita is preferably, it is Odontella aurita.
  • Odontella biomass can be crude, that is to say raw microlagues recovered after production.
  • Odontella can be done according to any suitable process.
  • the production of Odontella is carried out in an open environment (especially in raceways) exposed to the sun (photoautotrophy) but also in closed environments in photobioreactors or in plastic bags whose walls are transparent (photoautotrophy). ).
  • Some strains may also be produced in fermentor bioreactors by heterotrophy / mixotrophy.
  • the raw biomass may be treated prior to its use in the food products according to the invention or before it is used to obtain extracts which are themselves then used in products intended for food.
  • the pretreatment of the biomass is preferably a wash in particular to reduce its salt content and preferably sodium. It may be in particular a method which consists of the implementation of the following steps:
  • the biomass previously treated or not, is incorporated in the product intended for feeding or is used to obtain an extract.
  • the extract (s) contained in the products according to the invention may be of any nature and obtained by any suitable method.
  • the product according to the invention comprises a lipid extract of Odontella.
  • This extract may be used alone or with at least one or more other Odontella extracts (for example a hydrophilic extract) and / or Odontella biomass and / or one or more other extracts of other microalgae or cyanobacteria in the food product or beverage.
  • the lipid extract preferably comprises:
  • neutral lipids in particular glycerides, mono-, di- and triglycerides, free fatty acids and sterols,
  • polar lipids in particular glycolipids and phospholipids, and / or lipophilic pigments, in particular carotenoids and chlorophylls.
  • the lipid extract specifically comprises EPA, DHA, fucoxanthin, carotenoid pigments, water-soluble polysaccharides and / or phospholipids.
  • the lipid extract is preferably in liquid form but may be in solid form, particularly in the form of an encapsulated powder.
  • the lipid extract may be obtained by extraction with supercritical C0 2 with or without use of a cosolvent (ethanol or ethyl acetate), and / or extraction with hot ethanol re- circulated from a biomass preferably pretreated by drying and / or washing and drying as previously described. This extraction may be followed by a microencapsulation step to allow the use of lipid extracts in an aqueous medium. It is possible to envisage a complementary phase of fractionation of the lipid extract for separation of phospholipids, neutral lipids (including fatty acids of nutritional interest), carotenoid pigments and / or sterols.
  • the products intended for feeding according to the invention may contain a hydrophilic extract.
  • This extract can be used alone or with at least one or more other Odontella extracts (for example a lipid extract) and / or Odontella biomass and / or one or more other extracts of other microalgae or cyanobacteria in the food product or drink.
  • the hydrophilic extract preferably comprises:
  • inorganic salts in particular organic silica and trace minerals, hydrophilic pigments, in particular polyphenols,
  • the hydrophilic extract specifically comprises organic silica, polyphenols and proteins.
  • the hydrophilic extract may be in solid form, especially in powder form, or in liquid form.
  • the hydrophilic extract may be obtained by extraction preferably with supercritical CO 2 with or without a co-solvent with low-toxicity solvents or GRAS solvents such as ethanol or ethyl acetate, from a biomass preferentially previously treated with drying and / or washing and drying as previously described. It is possible to envisage a complementary phase of fractionation of the extract.
  • the lipid and hydrophilic compounds can also be separated and recovered by a treatment in the liquid phase during the washing phase or following this desalination phase:
  • all the hydrophilic extracts comprise organic silica.
  • the hydrophilic extracts are particularly suitable for liquid products, in particular for drinks.
  • the products intended for consumption according to the invention are preferably obtained by mixing Odontella biomass in powder form and / or an Odontella extract in powder form or in liquid form.
  • Odontella protein extracts it is necessary to introduce them as a stable emulsion in liquid foods and as a dry powder in a solid food.
  • microalgae of the genus Odontella in foods or drinks according to the invention makes it possible on the one hand to provide the consumer with important nutrients for the maintenance of optimal health that compensate for the shortcomings of modern food, and on the other hand to bring a particular flavor to the foods or drinks in which they are integrated including a taste or aroma of ocean and / or marine sweet type slightly sweet, fresh and weakly iodized.
  • the invention therefore also relates to the use of biomass of a marine microalga of the genus Odontella and / or at least one extract of said microalga as booster and / or flavor enhancer in food products or beverages.
  • the products according to the invention may, in addition to marine microalgae of the genus Odontella, contain biomass or extracts of microalgae of the genus Chlorella and / or biomass or extracts of Spirulina.
  • a mixture makes it possible to rebalance the protein content, to incorporate phycocyanins and to bring back trace elements, minerals and vitamins, in particular Group B, which is less present in Odontella.
  • the invention is now illustrated by non-limiting examples.
  • This example is a sparkling beverage with a mild, slightly sweet, fresh and slightly iodized taste or aroma of ocean and / or sea-salt.
  • the drink contains extracts of Odontella:
  • an encapsulated lipidic extract providing polyunsaturated fatty acids a hydrophilic extract containing marine trace elements, organic silica, marine proteins including all essential acids and polysaccharides.
  • the drink also includes Spirulina and Chlorella.
  • the composition is presented in the table below: Ingredients Quantity
  • Hydrophilic extract of Spirulina 0.5 to 2 g
  • This example is a lactofermented product containing a hydrophilic Odontella solids content between 1 and 5% of the fresh weight of the product.
  • This extract contains marine trace elements, organic silica, marine proteins and polysaccharides, pigments, antioxidants.
  • the lactofermented product may also contain extracts of Spirulina and Chlorella.
  • This example is a dressing product such as vinaigrette or table oil containing:
  • This example is an aperitif biscuit containing dry biomass of Odontella, Chlorella and spirulina combined with extracts of Odontella rich in organic silica and proteins of marine origin.

Abstract

The invention is directed to a product intended for human or animal consumption in the form of a food product or beverage, comprising 0.2 to 50% by weight of a marine microalga biomass and/or of at least one extract of said microalga. The invention also concerns use of this marine microalga and/or of at least one extract thereof as a flavour enhancer and/or potentiator in food products or beverages.

Description

ALIMENT OU BOISSON  FOOD OR DRINK
A BASE D'UNE MICROALGUE MARINE  BASED ON A MARINE MICROALGUE
La présente invention se rapporte à des produits alimentaires et des boissons comprenant une microalgue marine. L'invention concerne également l'utilisation de cette microalgue marine comme rehausseur et/ou exhausteur de goût dans des aliments ou des boissons. The present invention relates to food products and beverages comprising a marine microalgae. The invention also relates to the use of this marine microalgae as a booster and / or flavor enhancer in foods or beverages.
Les microalgues sont des organismes aquatiques dulcicoles ou marins, qui possèdent une concentration élevée en éléments nutritifs d'excellente qualité, en particulier des protéines complètes, des acides gras polyinsaturés, des fibres et polysaccharides, des minéraux, des vitamines hydrosolubles, des pigments et des antioxydants. De plus, ce sont des organismes du premier niveau trophique qui accumulent donc moins de polluants et de composés nocifs. En outre, la production de microalgues se fait par culture ce qui n'implique pas de récolte dans le milieu naturel contrairement à l'obtention d'autres organismes aquatiques, et permet ainsi de mieux contrôler la qualité et la traçabilité des produits finis. Microalgae are freshwater or marine aquatic organisms with a high concentration of high quality nutrients, including whole proteins, polyunsaturated fatty acids, fiber and polysaccharides, minerals, water-soluble vitamins, pigments and antioxidants. In addition, they are first-tier organisms that accumulate fewer pollutants and harmful compounds. In addition, the production of microalgae is by culture which does not involve harvesting in the natural environment unlike obtaining other aquatic organisms, and thus allows better control of the quality and traceability of finished products.
Pour ces raisons, certaines d'entre elles sont proposées depuis quelques années comme compléments alimentaires, en particulier deux espèces d'une microalgue d'eau douce du genre Chlorella. C'est le cas également d'une cyanobactérie appelée spiruline.  For these reasons, some of them have been proposed in recent years as food supplements, in particular two species of a freshwater microalgae of the genus Chlorella. This is also the case of a cyanobacterium called spirulina.
Toutefois, ces microorganismes ne possèdent pas certaines molécules essentielles pour la santé en particulier des acides gras polyinsaturés à longue chaîne de la famille des oméga 3 qui sont aujourd'hui fournis par l'utilisation de produits d'origine animale (huile de poissons, de krill, de crevettes ou de mollusques notamment) donc non accessibles à une partie grandissante de la population (allergiques, végétariens, végétaliens, écologistes conscientisés, etc.). De plus, ces microorganismes ne présentent aucun intérêt gustatif dans les produits alimentaires ou es boissons. However, these microorganisms do not possess certain molecules that are essential for health, in particular long-chain polyunsaturated fatty acids from the omega 3 family that are today provided by the use of animal products (fish oil, krill, shrimps or molluscs especially) so not accessible to a growing part of the population (allergic, vegetarians, vegans, conscientious ecologists, etc.). In addition, these microorganisms do not have any taste interest in food products or beverages.
L'objectif de l'invention est de proposer des produits destinés à l'alimentation à base d'une matière première d'origine algale dont l'utilisation permet à la fois d'augmenter les teneurs en EPA et DHA, fucoxanthine et autres pigments antioxydants dans les produits alimentaires sans utiliser de produits d'origine animale, apporter de la silice organique et de rehausser et/ou améliorer le gout de ces produits en limitant la quantité de sodium qu'ils contiennent. A cet effet, l'invention vise l'utilisation d'une microalgue marine et d'extrait d'une microalgue du genre Odontella dans des produits alimentaires. The objective of the invention is to propose products intended for feeding on the basis of a raw material of algal origin, the use of which makes it possible at the same time to increase the levels of EPA and DHA, fucoxanthin and other pigments. antioxidants in food products without using products of animal origin, provide organic silica and enhance and / or improve the taste of these products by limiting the amount of sodium they contain. To this end, the invention relates to the use of a marine microalgae and extract of a microalga Odontella genus in food products.
Les microalgues marines du genre Odontella sont connues. Leur utilisation en particulier en cosmétique a été décrit comme par exemple dans FR2918564, FR2833171. Marine microalgae of the genus Odontella are known. Their use in particular in cosmetics has been described as for example in FR2918564, FR2833171.
Avantageusement, leur utilisation selon l'invention permet d'augmenter la teneur en EPA et DHA dans les produits alimentaires, ainsi que d'autres molécules comme la fucoxanthine, des caroténoïdes à fort pouvoir oxydant et de la silice organique qui s'intègre à la matrice osseuse et permet d'obtenir des os plus durs et résistants. De façon surprenante, l'utilisation d'Odontella dans des produits alimentaires permet d'en rehausser le goût et apporte des saveurs nouvelles qui renforcent les arômes et les goûts marins et augmentent la qualité organoleptique du produit. Advantageously, their use according to the invention makes it possible to increase the content of EPA and DHA in food products, as well as other molecules such as fucoxanthin, carotenoids with high oxidizing power and organic silica that integrates with the bone matrix and allows for more hard and resistant bones. Surprisingly, the use of Odontella in food products enhances the taste and brings new flavors that enhance the flavors and tastes of the sea and increase the organoleptic quality of the product.
En particulier, l'invention a pour objet un produit destiné à la consommation humaine ou animale, sous forme d'aliment ou de boisson, comprenant entre 0,2 et 50% en poids de biomasse d'une microalgue marine du genre Odontella et/ou d'au moins un extrait de ladite microalgue.  In particular, the subject of the invention is a product intended for human or animal consumption, in the form of food or drink, comprising between 0.2 and 50% by weight of biomass of a marine microalgae of the genus Odontella and or at least one extract of said microalgae.
L'invention vise également l'utilisation de biomasse d'une microalgue marine du genre Odontella et/ou d'au moins un extrait de ladite microalgue comme rehausseur et/ou exhausteur de goût dans des produits alimentaires ou des boissons.  The invention also relates to the use of biomass of a marine microalgae of the genus Odontella and / or at least one extract of said microalga as enhancer and / or flavor enhancer in food products or beverages.
L'invention est à présent décrite en détails. The invention is now described in detail.
Définitions Definitions
Par « biomasse » d'une microalgue marine du genre Odontella au sens de l'invention on entend la matière cellulaire entière.  By "biomass" of a marine microalgae of the genus Odontella within the meaning of the invention is meant the entire cellular material.
Par « extrait » au sens de l'invention on entend tout mélange de plusieurs molécules natives contenues dans la biomasse d'Odontella, obtenu par un ou des procédés d'extractions. Par effet « rehausseur et/ou exhausteur de gout » au sens de l'invention on entend un goût et des arômes typiquement marins et/ou un goût plus prononcé d'arômes marins qui augmente les qualités organoleptiques du produit. Description détaillée de l'invention By "extract" within the meaning of the invention is meant any mixture of several native molecules contained in the Odontella biomass, obtained by one or more extraction methods. By effect "booster and / or flavor enhancer" within the meaning of the invention means a taste and typically marine flavors and / or a more pronounced taste of marine aroma that increases the organoleptic qualities of the product. Detailed description of the invention
L'invention a pour objet un produit destiné à la consommation humaine ou animale, sous forme d'aliment ou de boisson, comprenant entre 0,2 et 50% en poids d'une microalgue marine du genre Odontella.  The subject of the invention is a product intended for human or animal consumption, in the form of food or drink, comprising between 0.2 and 50% by weight of a marine microalgae of the genus Odontella.
Le produit destiné à la consommation selon l'invention peut être en particulier : The product intended for consumption according to the invention can be in particular:
un aliment vétérinaire ou « pet food » (aliment pour animal domestique) destiné à la consommation animale comme par exemple un aliment spécifique à des phases d'élevage particulières de la croissance de l'animal (stade nurserie, reproduction, prévention des maladies croissance etc...)..  a veterinary food or "pet food" intended for animal consumption, for example a specific feed for particular growth phases of the animal (nursery stage, reproduction, prevention of diseases growth etc.) ...) ..
une boisson vétérinaire destinée à la consommation animale comme par exemple un complément alimentaire spécifique à des phases d'élevage particulières de la croissance de l'animal (stade nurserie, reproduction, prévention des maladies croissance etc.),  a veterinary drink intended for animal consumption, for example a dietary supplement specific to particular growth stages of the animal (nursery stage, reproduction, prevention of growth diseases, etc.),
un aliment destiné à la consommation humaine, comme par exemple un produit céréalier, un produit de boulangerie, un gâteau sucré ou salé, un biscuit sucré ou salé, une sucrerie, une soupe, une chips de légumes, un condiment, une huile, un plat cuisiné, un produit transformé à partir de produits marins (terrine, surimis, etc.), un laitage ou un liquide issu de végétaux fermenté ou non,  a food intended for human consumption, such as a cereal product, a bakery product, a sweet or savory cake, a sweet or savory biscuit, a candy, a soup, a vegetable crisps, a condiment, an oil, a cooked dish, a product processed from marine products (terrine, surimis, etc.), a dairy product or a liquid derived from plants fermented or not,
une boisson destinée à la consommations humaine, comme par exemple un soda, un jus de fruits, une boisson énergétique, une eau aromatisée, un sirop ou une boisson alcoolisée.  a drink for human consumption, such as a soda, a fruit juice, an energy drink, flavored water, a syrup or an alcoholic beverage.
Le produit selon l'invention contient entre 0,2 et 50% en poids : The product according to the invention contains between 0.2 and 50% by weight:
de biomasse d'une microalgue marine du genre Odontella, et/ou  biomass of a marine microalga of the genus Odontella, and / or
d'au moins un extrait d'une microalgue marine du genre Odontella.  at least one extract of a marine microalgae of the genus Odontella.
La ou les microalgue(s) marine(s) présente(nt) dans les produits selon l'invention peu(ven)t être de toute espèce appartenant au genre Odontella, telles que Odontella aurita, Odontella granulata, Odontella longicruris, Odontella mobiliensis, Odontella régla Odontella rhombus, Odontella simensis. Préférentiellement, il s'agit d'Odontella aurita. The marine microalgae (s) present in the products according to the invention may be of any species belonging to the genus Odontella, such as Odontella aurita, Odontella granulata, Odontella longicruris, Odontella mobiliensis, Odontella regulated Odontella rhombus, Odontella simensis. Preferably, it is Odontella aurita.
La biomasse d'Odontella peut-être brute c'est-à-dire des microlagues brutes récupérées après production.  Odontella biomass can be crude, that is to say raw microlagues recovered after production.
La production d'Odontella peut se faire selon tout procédé adapté. En particulier, la production d'Odontella est réalisée en milieu ouvert (en particulier dans des bassins de type « raceways ») exposés au soleil (photoautotrophie) mais aussi en milieu fermé en photobioréacteurs ou en sacs de plastique dont les parois sont transparentes (photoautotrophie). Certaines souches peuvent être également produites en bioréacteurs de type fermenteur par hétérotrophie/mixotrophie.  The production of Odontella can be done according to any suitable process. In particular, the production of Odontella is carried out in an open environment (especially in raceways) exposed to the sun (photoautotrophy) but also in closed environments in photobioreactors or in plastic bags whose walls are transparent (photoautotrophy). ). Some strains may also be produced in fermentor bioreactors by heterotrophy / mixotrophy.
La biomasse brute peut être traitée préalablement à son utilisation dans les produits destinés à l'alimentation selon l'invention ou avant qu'elle soit utilisée pour obtenir des extraits qui sont eux-mêmes ensuite utilisés dans les produits destinés à l'alimentation. The raw biomass may be treated prior to its use in the food products according to the invention or before it is used to obtain extracts which are themselves then used in products intended for food.
Le traitement préalable de la biomasse est préférentiellement un lavage notamment pour diminuer sa teneur en sels et préférentiellement le sodium. Il peut s'agir en particulier d'un procédé qui consiste en la mise en œuvre des étapes suivantes : The pretreatment of the biomass is preferably a wash in particular to reduce its salt content and preferably sodium. It may be in particular a method which consists of the implementation of the following steps:
Centrifugation d'une biomasse d'Odontella, notamment avec une centrifugeuse verticale à plateaux deux phases et/ou une centrifugeuse horizontale,  Centrifugation of an Odontella biomass, in particular with a vertical centrifuge with two-phase trays and / or a horizontal centrifuge,
Mise en suspension sous agitation pendant 5 à 30 minutes,  Suspending with stirring for 5 to 30 minutes,
Séparation des phases solide et liquide préférentiellement par centrifugation (centrifugeuse deux phases) et/ou par filtration, notamment avec une filtration tangentielle (microfiltration et/ou ultra filtration et/ou nanofiltration),  Separation of the solid and liquid phases preferably by centrifugation (two-phase centrifuge) and / or by filtration, in particular with tangential filtration (microfiltration and / or ultra filtration and / or nanofiltration),
Récupération de la phase solide et séchage préférentiellement par lyophilisation et/ou zéodratation. Un séchage par atomisation et/ou spray dryer peut également être utilisé.  Recovery of the solid phase and drying preferably by lyophilization and / or zeodration. Spray drying and / or spray dryer may also be used.
La biomasse, préalablement traitée ou non, est incorporée dans le produit destiné à l'alimentation ou est utilisée pour obtenir un extrait. The biomass, previously treated or not, is incorporated in the product intended for feeding or is used to obtain an extract.
Le ou les extrait(s) contenu(s) dans les produits selon l'invention peuvent être de toute nature et obtenus par tout procédé adapté.  The extract (s) contained in the products according to the invention may be of any nature and obtained by any suitable method.
Selon un mode de réalisation adapté, le produit selon l'invention comprend un extrait lipidique d'Odontella. Cet extrait peut être utilisé seul ou avec au moins un ou plusieurs autres extraits d'Odontella (par exemple un extrait hydrophile) et/ou de la biomasse d'Odontella et/ou un ou plusieurs autres extraits d'autres microalgues ou cyanobactéries dans le produit alimentaire ou la boisson. According to a suitable embodiment, the product according to the invention comprises a lipid extract of Odontella. This extract may be used alone or with at least one or more other Odontella extracts (for example a hydrophilic extract) and / or Odontella biomass and / or one or more other extracts of other microalgae or cyanobacteria in the food product or beverage.
L'extrait lipidique comprend préférentiellement :  The lipid extract preferably comprises:
des lipides neutres, en particulier des glycérides, mono, di et triglycérides, des acides gras libres et des stérols,  neutral lipids, in particular glycerides, mono-, di- and triglycerides, free fatty acids and sterols,
des lipides polaires, en particulier des glycolipides et phospholipides, et/ou des pigments lipophiles, en particulier des caroténoïdes et des chlorophylles.  polar lipids, in particular glycolipids and phospholipids, and / or lipophilic pigments, in particular carotenoids and chlorophylls.
Encore plus préférentiellement l'extrait lipidique comprend spécifiquement de l'EPA, du DHA, de la fucoxanthine, des pigments caroténoïdes, des polysaccharides hydrosolubles et/ou des phospholipides.  Even more preferably, the lipid extract specifically comprises EPA, DHA, fucoxanthin, carotenoid pigments, water-soluble polysaccharides and / or phospholipids.
L'extrait lipidique se présente préférentiellement sous forme liquide mais peut se présenter sous forme solide en particulier sous forme de poudre encapsulée.  The lipid extract is preferably in liquid form but may be in solid form, particularly in the form of an encapsulated powder.
L'extrait lipidique peut-être obtenu par extraction au C02 supercritique avec ou sans utilisation d'un cosolvant (éthanol ou acétate d'éthyle), et/ou extraction à l'éthanol chaud re- circulé, à partir d'une biomasse préférentiellement préalablement traitée par séchage et/ou par lavage et séchage comme précédemment décrit. Cette extraction peut-être suivie d'une étape de micro encapsulation pour permettre l'utilisation des extraits lipidiques en milieu aqueux. Il est possible d'envisager une phase complémentaire de fractionnement de l'extrait lipidique pour séparation des phospholipides, des lipides neutres (dont les acides gras d'intérêts nutritionnels), des pigments caroténoïdes et/ou des stérols. The lipid extract may be obtained by extraction with supercritical C0 2 with or without use of a cosolvent (ethanol or ethyl acetate), and / or extraction with hot ethanol re- circulated from a biomass preferably pretreated by drying and / or washing and drying as previously described. This extraction may be followed by a microencapsulation step to allow the use of lipid extracts in an aqueous medium. It is possible to envisage a complementary phase of fractionation of the lipid extract for separation of phospholipids, neutral lipids (including fatty acids of nutritional interest), carotenoid pigments and / or sterols.
Selon une variante, les produits destinés à l'alimentation selon l'invention peuvent contenir un extrait hydrophile. Cet extrait peut être utilisé seul ou avec au moins un ou plusieurs autres extraits d'Odontella (par exemple un extrait lipidique) et/ou de la biomasse d'Odontella et/ou un ou plusieurs autres extraits d'autres microalgues ou cyanobactéries dans le produit alimentaire ou la boisson.  According to one variant, the products intended for feeding according to the invention may contain a hydrophilic extract. This extract can be used alone or with at least one or more other Odontella extracts (for example a lipid extract) and / or Odontella biomass and / or one or more other extracts of other microalgae or cyanobacteria in the food product or drink.
L'extrait hydrophile comprend préférentiellement :  The hydrophilic extract preferably comprises:
des protéines,  proteins,
des acides aminés libres  free amino acids
des sels minéraux, en particulier de la silice organique et des oligo-minéraux, - des pigments hydrophiles, en particulier des polyphénols,  inorganic salts, in particular organic silica and trace minerals, hydrophilic pigments, in particular polyphenols,
des polysaccharides,  polysaccharides,
et/ou des fibres. Encore plus préférentiellement l'extrait hydrophile comprend spécifiquement de la silice organique, des polyphénols et des protéines. and / or fibers. Even more preferably, the hydrophilic extract specifically comprises organic silica, polyphenols and proteins.
L'extrait hydrophile peut se présenter sous forme solide, notamment sous forme de poudre, ou sous forme liquide.  The hydrophilic extract may be in solid form, especially in powder form, or in liquid form.
L'extrait hydrophile peut-être obtenu par extraction préférentiellement au C02 supercritique avec ou sans co-solvant avec des solvants peu toxiques ou G.R.A.S comme l'éthanol ou l'acétate d'éthyl, à partir d'une biomasse préférentiellement préalablement traitée par séchage et/ou par lavage et séchage comme précédemment décrit. Il est possible d'envisager une phase complémentaire de fractionnement de l'extrait. The hydrophilic extract may be obtained by extraction preferably with supercritical CO 2 with or without a co-solvent with low-toxicity solvents or GRAS solvents such as ethanol or ethyl acetate, from a biomass preferentially previously treated with drying and / or washing and drying as previously described. It is possible to envisage a complementary phase of fractionation of the extract.
Les composés lipidiques et hydrophiles peuvent aussi être séparer et récupérer par un traitement en phase liquide lors de la phase de lavage ou suite à cette phase de dessalement : The lipid and hydrophilic compounds can also be separated and recovered by a treatment in the liquid phase during the washing phase or following this desalination phase:
Mise en suspension sous agitation des cellules de microalgues pendant 5 à 30 minutes, permettant d'appliquer un traitement physique (microondes et/ou ultrasons et/ou chocs thermiques et/ou chocs osmotiques) et/ou un traitement enzymatique (enzymes protéolytiques et/ou enzymes lipolytiques et/ou d'enzymes responsables de la lyse des polysaccharides membranaires). Séparation des phases solides et des phases liquides (aqueuse / lipidique) préférentiellement par centrifugation (centrifugeuse trois phase) et/ou par filtration, notamment avec une filtration tangentielle (microfiltration et/ou ultrafiltration et/ou nanofiltration). - Récupération de la phase solide et séchage préférentiellement par lyophilisation et/ou zéodratation. Un séchage par atomisation et/ou spray dryer peut également être utilisé.  Stirring of the microalgae cells with stirring for 5 to 30 minutes, making it possible to apply a physical treatment (microwaves and / or ultrasounds and / or thermal shocks and / or osmotic shocks) and / or an enzymatic treatment (proteolytic enzymes and / or or lipolytic enzymes and / or enzymes responsible for lysing membrane polysaccharides). Separation of solid phases and liquid phases (aqueous / lipid) preferably by centrifugation (three-phase centrifuge) and / or by filtration, in particular with tangential filtration (microfiltration and / or ultrafiltration and / or nanofiltration). - Recovery of the solid phase and drying preferably by lyophilization and / or zeodration. Spray drying and / or spray dryer may also be used.
Récupération de la phase liquide lipidique (huile et graisse) qui pourra être utilisée sans traitement particulier autre que l'ajout de composés antioxydants (vitamines E, extraits de plantes aromatiques, etc.) : huile brute ou être raffinée selon les techniques de l'art (purification, décoloration, désodorisation, ajout de composés antioxydants (vitamines E, extraits de plantes aromatiques, etc.) : huile raffinée ou partiellement raffinée.  Recovery of the lipid liquid phase (oil and fat) which can be used without any special treatment other than the addition of antioxidant compounds (vitamins E, aromatic plant extracts, etc.): crude oil or be refined according to the techniques of the art (purification, discoloration, deodorization, addition of antioxidant compounds (vitamin E, aromatic plant extracts, etc.): refined or partially refined oil.
Préférentiellement, tous les extraits hydrophiles comprennent de la silice organique. Preferentially, all the hydrophilic extracts comprise organic silica.
Les extraits hydrophiles sont particulièrement adaptés aux produits liquides en particulier aux boissons. Les produits destinés à la consommation selon l'invention sont préférentiellement obtenus par mélange de biomasse d'Odontella sous forme de poudre et/ou d'un extrait d'Odontella sous forme de poudre ou sous forme liquide. Dans le cas où il s'agit d'un extrait lipidique encapsulé, il peut être introduit dans les produits liquides et solides. Pour les extraits protéiniques d'Odontella, il est nécessaire pour les introduire sous forme d'émulsion stable dans les aliments liquides et sous forme de poudre sèche dans un aliment solide. The hydrophilic extracts are particularly suitable for liquid products, in particular for drinks. The products intended for consumption according to the invention are preferably obtained by mixing Odontella biomass in powder form and / or an Odontella extract in powder form or in liquid form. In the case where it is an encapsulated lipid extract, it can be introduced into liquid and solid products. For the Odontella protein extracts, it is necessary to introduce them as a stable emulsion in liquid foods and as a dry powder in a solid food.
La présence de microalgues du genre Odontella dans des aliments ou des boissons selon l'invention permet d'une part d'apporter au consommateur des nutriments importants pour le maintien d'une santé optimale qui compensent les lacunes de l'alimentation moderne, et d'autre part d'apporter une saveur particulière aux aliments ou aux boissons dans lesquels ils sont intégrés notamment un goût ou arôme d'océan et/ou marin de type doux légèrement sucré, frais et faiblement iodé. The presence of microalgae of the genus Odontella in foods or drinks according to the invention makes it possible on the one hand to provide the consumer with important nutrients for the maintenance of optimal health that compensate for the shortcomings of modern food, and on the other hand to bring a particular flavor to the foods or drinks in which they are integrated including a taste or aroma of ocean and / or marine sweet type slightly sweet, fresh and weakly iodized.
L'invention vise donc également l'utilisation de biomasse d'une microalgue marine du genre Odontella et/ou d'au moins un extrait de ladite microalgue comme rehausseur et/ou exhausteur de goût dans des produits alimentaires ou des boissons.  The invention therefore also relates to the use of biomass of a marine microalga of the genus Odontella and / or at least one extract of said microalga as booster and / or flavor enhancer in food products or beverages.
Les produits selon l'invention peuvent, en plus des microalgues marines du genre Odontella, contenir de la biomasse ou des extraits de microalgues du genre Chlorella et/ou de la biomasse ou des extraits de Spiruline. Avantageusement, un tel mélange permet de rééquilibrer la teneur en protéines, incorporer des phycocyanines et ramener des oligo- éléments, minéraux et vitamines notamment du groupe B moins présent chez Odontella. L'invention est à présent illustrée par des exemples non limitatifs.  The products according to the invention may, in addition to marine microalgae of the genus Odontella, contain biomass or extracts of microalgae of the genus Chlorella and / or biomass or extracts of Spirulina. Advantageously, such a mixture makes it possible to rebalance the protein content, to incorporate phycocyanins and to bring back trace elements, minerals and vitamins, in particular Group B, which is less present in Odontella. The invention is now illustrated by non-limiting examples.
Exemples : Examples:
Exemple 1 : Boisson pétillante  Example 1: Sparkling drink
Cet exemple est une boisson pétillante au goût ou arôme d'océan et/ou marin de type doux légèrement sucré, frais et faiblement iodé. La boisson contient des extraits d'Odontella : This example is a sparkling beverage with a mild, slightly sweet, fresh and slightly iodized taste or aroma of ocean and / or sea-salt. The drink contains extracts of Odontella:
un extrait lipidique encapsulé (poudre) apportant des acides gras polyinsaturés un extrait hydrophile contenant des oligoéléments marins , de la silice organique, des protéines d'origines marine dont tous les acides essentiels et des polysaccharides.  an encapsulated lipidic extract (powder) providing polyunsaturated fatty acids a hydrophilic extract containing marine trace elements, organic silica, marine proteins including all essential acids and polysaccharides.
La boisson comprend également de la spiruline et Chlorella. La composition est présentée dans le tableau ci-après : Ingrédients Quantité The drink also includes Spirulina and Chlorella. The composition is presented in the table below: Ingredients Quantity
Eau de source pétillante 490 g  Sparkling spring water 490 g
Extrait hydrophile de microalgue 5 à 10 g Hydrophilic extract of microalgae 5 to 10 g
marine micro-encapsulé du  micro-encapsulated marine
genre Odontella  Odontella kind
Extraits naturels Bio de fraise 2 à 4 g  Bio natural strawberry extracts 2 to 4 g
Ou Extraits naturels Bio de 2 à 4 g  Or organic extracts of 2 to 4 g
framboise  raspberry
Ou Extraits naturels Bio de 2 à 4 g  Or organic extracts of 2 to 4 g
cerise  cherry
Ou Extraits naturels Bio 2 à 4 g  Or Organic Extracts 2 to 4 g
d'hibiscus  hibiscus
Extrait concentré de romarin de 0,5 à 1 g  Concentrated rosemary extract from 0.5 to 1 g
type Naturex (antioxydant  Naturex type (antioxidant
puissant)  powerful)
Extrait hydrophile de Spiruline 0,5 à 2 g  Hydrophilic extract of Spirulina 0.5 to 2 g
et / ou de Chlorelle  and / or Chlorella
Sucre de canne Bio 2 à 5 g  Organic cane sugar 2 to 5 g
Exemple 2 : Yaourts et préparations végétales lactofermentées Example 2 Yogurts and Plant Preparations Lactofermented
Cet exemple est un produit lactofermenté contenant un extrait sec hydrophile d'Odontella entre 1 et 5 % du poids frais du produit. Cet extrait contenant des oligoéléments marins, de la silice organique, des protéines d'origines marine et des polysaccharides, des pigments, des antioxydants. Le produit lactofermenté peut également contenir des extraits de spiruline et Chlorella. This example is a lactofermented product containing a hydrophilic Odontella solids content between 1 and 5% of the fresh weight of the product. This extract contains marine trace elements, organic silica, marine proteins and polysaccharides, pigments, antioxidants. The lactofermented product may also contain extracts of Spirulina and Chlorella.
Exemple 3 : Vinaigrette Example 3: Vinaigrette
Cet exemple est un produit d'assaisonnement de type vinaigrette ou huile de table contenant :  This example is a dressing product such as vinaigrette or table oil containing:
une émulsion stable d'extrait lipidique d'Odontella et de Chlorella comprenant des acides gras polyinsaturés et des phospholipides,  a stable emulsion of Odontella and Chlorella lipid extract comprising polyunsaturated fatty acids and phospholipids,
- Un extrait sec hydrophile d'Odontella contenant des oligoélements marins et de la silice organique. Exemple 4 : Biscuit apéritif - A hydrophilic dry extract of Odontella containing marine oligoelements and organic silica. Example 4: Aperitif biscuit
Cet exemple est un biscuit apéritif contenant de la biomasse sèche d'Odontella, Chlorella et spiruline associé à des extraits d'Odontella riches en silice organique et protéines d'origines marine.  This example is an aperitif biscuit containing dry biomass of Odontella, Chlorella and spirulina combined with extracts of Odontella rich in organic silica and proteins of marine origin.

Claims

REVENDICATIONS
1. Produit destiné à la consommation humaine ou animale, sous forme d'aliment ou de boisson, comprenant entre 0,2 et 50% en poids de biomasse d'une microalgue marine du genre Odontella et/ou d'au moins un extrait de ladite microalgue. 1. Product intended for human or animal consumption, in the form of food or drink, comprising between 0.2% and 50% by weight of biomass of a marine microalgae of the genus Odontella and / or at least one extract of said microalgae.
2. Produit selon la revendication 1, caractérisé en ce qu'il s'agit d'un produit céréalier, d'un produit de boulangerie, d'un gâteau sucré ou salé, d'un biscuit sucré ou salé, d'une sucrerie, d'une soupe, d'une chips de légumes, d'un condiment, d'une huile, d'un plat cuisiné, d'un produit transformé à partir de produits marins, un laitage.  2. Product according to claim 1, characterized in that it is a cereal product, a bakery product, a sweet or salty cake, a sweet or savory biscuit, a candy , a soup, a vegetable crisps, a condiment, an oil, a cooked dish, a product transformed from marine products, a dairy.
3. Produit selon la revendication 1, caractérisé en ce qu'il s'agit d'un soda, d'un jus de fruits, d'une boisson énergétique, d'une eau aromatisée, d'un sirop ou d'une boisson alcoolisée.  3. Product according to claim 1, characterized in that it is a soda, a fruit juice, an energy drink, a flavored water, a syrup or a drink alcoholic.
4. Produit selon l'une des précédentes revendications, caractérisé en ce qu'il comprend un extrait lipidique d'Odontella.  4. Product according to one of the preceding claims, characterized in that it comprises a lipid extract of Odontella.
5. Produit selon la précédente revendication, caractérisé en ce que l'extrait lipidique comprend des lipides neutres, des lipides polaires et/ou des pigments lipophiles.  5. Product according to the preceding claim, characterized in that the lipid extract comprises neutral lipids, polar lipids and / or lipophilic pigments.
6. Produit selon l'une des revendications 4 ou 5, caractérisé en ce que l'extrait lipidique comprend de l'EPA, du DHA, de la fucoxanthine, des pigments caroténoïdes et/ou des phospholipides.  6. Product according to one of claims 4 or 5, characterized in that the lipid extract comprises EPA, DHA, fucoxanthin, carotenoid pigments and / or phospholipids.
7. Produit selon l'une des précédentes revendications, caractérisé en ce qu'il comprend un extrait hydrophile d'Odontella.  7. Product according to one of the preceding claims, characterized in that it comprises a hydrophilic Odontella extract.
8. Produit selon la précédente revendication, caractérisé en ce que l'extrait hydrophile comprend des protéines, des sels minéraux, des pigments hydrophiles, des polysaccharides et/ou des fibres.  8. Product according to the preceding claim, characterized in that the hydrophilic extract comprises proteins, mineral salts, hydrophilic pigments, polysaccharides and / or fibers.
9. Produit selon l'une des revendications 7 ou 8, caractérisé en ce que l'extrait hydrophile comprend de la silice organique, des polyphénols et des protéines.  9. Product according to one of claims 7 or 8, characterized in that the hydrophilic extract comprises organic silica, polyphenols and proteins.
10. Produit selon l'une des précédentes revendications, caractérisé en ce que la microalgue marine est Odontella aurita.  10. Product according to one of the preceding claims, characterized in that the marine microalgae is Odontella aurita.
11. Produit selon l'une des précédentes revendications, caractérisé en ce qu'il comprend de la biomasse d'Odontella et en ce que cette biomasse a subi un traitement préalable. 11. Product according to one of the preceding claims, characterized in that it comprises Odontella biomass and in that this biomass has undergone prior treatment.
12. Produit selon l'une des précédentes revendications, caractérisé en ce qu'il comprend un extrait d'Odontella obtenu à partir d'une biomasse d'Odontella qui a subi un traitement préalable. 12. Product according to one of the preceding claims, characterized in that it comprises an Odontella extract obtained from a Odontella biomass which has undergone prior treatment.
13. Produit selon la revendication 11 ou 12, caractérisé en ce que le traitement préalable de la biomasse d'Odontella consiste en la mise en œuvre des étapes suivantes :  13. Product according to claim 11 or 12, characterized in that the preliminary treatment of the Odontella biomass consists in the implementation of the following steps:
Centrifugation d'une biomasse d'Odontella,  Centrifugation of an Odontella biomass,
Mise en suspension sous agitation pendant 5 à 30 minutes,  Suspending with stirring for 5 to 30 minutes,
Séparation des phases solide et liquide par centrifugation et/ou filtration,  Separation of the solid and liquid phases by centrifugation and / or filtration,
Récupération de la phase solide et séchage.  Recovery of the solid phase and drying.
14. Produit selon l'une des précédentes revendications, caractérisé par une saveur particulière apportée par la présence d'Odontella.  14. Product according to one of the preceding claims, characterized by a particular flavor provided by the presence of Odontella.
15. Utilisation de biomasse d'une microalgue marine du genre Odontella et/ou d'au moins un extrait de ladite microalgue comme rehausseur et/ou exhausteur de goût dans des produits alimentaires ou des boissons.  15. Use of biomass of a marine microalga of the genus Odontella and / or at least one extract of said microalgae as an enhancer and / or flavor enhancer in food products or beverages.
EP17790968.6A 2016-10-05 2017-10-05 Food product or beverage containing a marine microalga Withdrawn EP3522738A1 (en)

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