EP3415158A3 - Di-résidus variables de répétition pour cibler des nucléotides - Google Patents

Di-résidus variables de répétition pour cibler des nucléotides Download PDF

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Publication number
EP3415158A3
EP3415158A3 EP18188064.2A EP18188064A EP3415158A3 EP 3415158 A3 EP3415158 A3 EP 3415158A3 EP 18188064 A EP18188064 A EP 18188064A EP 3415158 A3 EP3415158 A3 EP 3415158A3
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EP
European Patent Office
Prior art keywords
present
repeat variable
targeting nucleotides
proteins
variable diresidues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18188064.2A
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German (de)
English (en)
Other versions
EP3415158A2 (fr
EP3415158B1 (fr
Inventor
Philippe Duchateau
Alexandre Juillerat
Claudia BERTONATI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectis SA
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Cellectis SA
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Publication of EP3415158A3 publication Critical patent/EP3415158A3/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP18188064.2A 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides Active EP3415158B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20206278.2A EP3799878A3 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261611360P 2012-03-15 2012-03-15
EP13727329.8A EP2825190B1 (fr) 2012-03-15 2013-03-15 Dirésidus variables de répétition pour cibler des nucléotides
PCT/IB2013/000734 WO2013136175A2 (fr) 2012-03-15 2013-03-15 Nouveaux dirésidus variables de répétition pour cibler des nucléotides
EP16193940.0A EP3141259B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition (rvd) pour cibler des nucléotides

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
EP16193940.0A Division-Into EP3141259B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition (rvd) pour cibler des nucléotides
EP16193940.0A Division EP3141259B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition (rvd) pour cibler des nucléotides
EP13727329.8A Division EP2825190B1 (fr) 2012-03-15 2013-03-15 Dirésidus variables de répétition pour cibler des nucléotides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP20206278.2A Division EP3799878A3 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides

Publications (3)

Publication Number Publication Date
EP3415158A2 EP3415158A2 (fr) 2018-12-19
EP3415158A3 true EP3415158A3 (fr) 2019-01-16
EP3415158B1 EP3415158B1 (fr) 2020-11-11

Family

ID=48577141

Family Applications (4)

Application Number Title Priority Date Filing Date
EP16193940.0A Active EP3141259B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition (rvd) pour cibler des nucléotides
EP20206278.2A Pending EP3799878A3 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides
EP13727329.8A Active EP2825190B1 (fr) 2012-03-15 2013-03-15 Dirésidus variables de répétition pour cibler des nucléotides
EP18188064.2A Active EP3415158B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides

Family Applications Before (3)

Application Number Title Priority Date Filing Date
EP16193940.0A Active EP3141259B1 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition (rvd) pour cibler des nucléotides
EP20206278.2A Pending EP3799878A3 (fr) 2012-03-15 2013-03-15 Di-résidus variables de répétition pour cibler des nucléotides
EP13727329.8A Active EP2825190B1 (fr) 2012-03-15 2013-03-15 Dirésidus variables de répétition pour cibler des nucléotides

Country Status (4)

Country Link
US (1) US11778993B2 (fr)
EP (4) EP3141259B1 (fr)
DK (3) DK2825190T3 (fr)
WO (1) WO2013136175A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4421167A2 (fr) 2016-12-16 2024-08-28 B-Mogen Biotechnologies, Inc. Transfert de gènes médié par transposon de la famille hoboat amélioré et compositions, systèmes et procédés associés
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
WO2018118585A1 (fr) * 2016-12-22 2018-06-28 Agenovir Corporation Compositions antivirales
CN107502621B (zh) * 2017-09-22 2021-04-13 福建农林大学 一种快速检测体内dna末端连接的方法
US11760983B2 (en) 2018-06-21 2023-09-19 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
CN113403341B (zh) * 2021-06-21 2022-04-19 南京医科大学 一种基于tale组装的线粒体dna编辑系统

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110145940A1 (en) * 2009-12-10 2011-06-16 Voytas Daniel F Tal effector-mediated dna modification
US20110239315A1 (en) * 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
WO2011146121A1 (fr) * 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Nouvelles protéines se liant à l'adn et leurs utilisations
WO2012138939A1 (fr) * 2011-04-05 2012-10-11 Philippe Duchateau Nouveaux échafaudages de protéine tale et leurs utilisations
US20130196373A1 (en) * 2011-11-16 2013-08-01 Sangamo Biosciences, Inc. Modified dna-binding proteins and uses thereof
WO2014078819A2 (fr) * 2012-11-16 2014-05-22 Transposagen Biopharmaceuticals, Inc. Enzymes spécifiques de site et procédés d'utilisation associés

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JP4966006B2 (ja) 2003-01-28 2012-07-04 セレクティス カスタムメイドメガヌクレアーゼおよびその使用
US8771985B2 (en) * 2010-04-26 2014-07-08 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using zinc-finger nucleases
US20130337454A1 (en) 2010-10-27 2013-12-19 Philippe Duchateau Method for increasing the efficiency of double-strand break-induced mutagenesis
US20130137173A1 (en) * 2011-11-30 2013-05-30 Feng Zhang Nucleotide-specific recognition sequences for designer tal effectors

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110239315A1 (en) * 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US20110145940A1 (en) * 2009-12-10 2011-06-16 Voytas Daniel F Tal effector-mediated dna modification
WO2011146121A1 (fr) * 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Nouvelles protéines se liant à l'adn et leurs utilisations
WO2012138939A1 (fr) * 2011-04-05 2012-10-11 Philippe Duchateau Nouveaux échafaudages de protéine tale et leurs utilisations
US20130196373A1 (en) * 2011-11-16 2013-08-01 Sangamo Biosciences, Inc. Modified dna-binding proteins and uses thereof
WO2014078819A2 (fr) * 2012-11-16 2014-05-22 Transposagen Biopharmaceuticals, Inc. Enzymes spécifiques de site et procédés d'utilisation associés

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BOCH JENS ET AL: "Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US, vol. 326, no. 5959, 1 December 2009 (2009-12-01), pages 1509 - 1512, XP002570745, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1178811 *
CONG LE ET AL: "Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains.", NATURE COMMUNICATIONS 2012, vol. 3, 2012, pages 968, XP002700145, ISSN: 2041-1723 *
FENG ZHANG ET AL: "Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription", NATURE BIOTECHNOLOGY, vol. 29, no. 2, 1 February 2011 (2011-02-01), pages 149 - 153, XP055005146, ISSN: 1087-0156, DOI: 10.1038/nbt.1775 *
M. M. MAHFOUZ ET AL: "De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 6, 8 February 2011 (2011-02-08), pages 2623 - 2628, XP055007615, ISSN: 0027-8424, DOI: 10.1073/pnas.1019533108 *
MOSCOU MATTHEW J ET AL: "A simple cipher governs DNA recognition by TAL effectors", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US, vol. 326, no. 5959, 11 December 2009 (2009-12-11), pages 1501, XP002599998, ISSN: 0036-8075 *
STREUBEL JANA ET AL: "TAL effector RVD specificities and efficiencies.", NATURE BIOTECHNOLOGY JUL 2012, vol. 30, no. 7, July 2012 (2012-07-01), pages 593 - 595, XP002700146, ISSN: 1546-1696 *

Also Published As

Publication number Publication date
EP3799878A2 (fr) 2021-04-07
EP2825190B1 (fr) 2016-11-30
DK2825190T3 (en) 2017-02-27
US11778993B2 (en) 2023-10-10
EP3141259B1 (fr) 2019-07-31
EP2825190A2 (fr) 2015-01-21
DK3141259T3 (da) 2019-09-30
EP3141259A2 (fr) 2017-03-15
EP3141259A3 (fr) 2017-04-19
DK3415158T3 (da) 2021-01-11
EP3415158A2 (fr) 2018-12-19
US20150067900A1 (en) 2015-03-05
WO2013136175A3 (fr) 2013-11-21
WO2013136175A2 (fr) 2013-09-19
EP3415158B1 (fr) 2020-11-11
EP3799878A3 (fr) 2021-07-14

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