EP3409369B1 - Dispositif de perturbation de substances biologiques - Google Patents

Dispositif de perturbation de substances biologiques Download PDF

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Publication number
EP3409369B1
EP3409369B1 EP18000339.4A EP18000339A EP3409369B1 EP 3409369 B1 EP3409369 B1 EP 3409369B1 EP 18000339 A EP18000339 A EP 18000339A EP 3409369 B1 EP3409369 B1 EP 3409369B1
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EP
European Patent Office
Prior art keywords
disrupting
fixed
biologic substances
grid
biologic
Prior art date
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Application number
EP18000339.4A
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German (de)
English (en)
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EP3409369A1 (fr
Inventor
Armando Roggero
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CTSV Srl
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CTSV Srl
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/062—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives with rotor elements extending axially in close radial proximity of a concentrically arranged slotted or perforated ring
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/20—Disintegrating by grating

Definitions

  • the present invention refers to a disruptor of biologic substances.
  • the present invention is in the field of preparing samples for physical, biophysical, bio-chemical assays, with mechanical working, for example, cutting, disintegrating, compacting, homogenizing.
  • the present invention deals with the disintegration of biologic tissues, the dissociation of cells and micro-grafts, with knives or other cutters which cut the material into fragments, with rotary knives inside vertical containers, with actuation arranged above the container.
  • IT-B-0001320860 discloses a disrupting device, preferably of the disposable type, suitable for treating disruptions of biologic samples and, in particular, for preparing homogenized samples, comprising a container shaped as a plastic glass, equipped with a cover with a central hole and a peripheral hole, a central rotating shaft connected to a mincing member, for example, of the type with one or more blades, supported by the bottom of the container through a spherical coupling and equipped with an engagement member to be able to join a drive shaft, and means to allow sucking the disrupted sample.
  • a disrupting device preferably of the disposable type, suitable for treating disruptions of biologic samples and, in particular, for preparing homogenized samples, comprising a container shaped as a plastic glass, equipped with a cover with a central hole and a peripheral hole, a central rotating shaft connected to a mincing member, for example, of the type with one or more blades, supported by the bottom of the container through a spherical coupling and equipped
  • WO-A2-2016097960 discloses a disrupting device for biologic material, comprising an external hollow body, defining an internal chamber, a fixed disrupting grid having a plurality of micro-holes equipped with cutting edges and transversally housed in the internal chamber in order to define an upper loading chamber adapted to be loaded with biologic material to be disrupted and a lower collecting chamber adapted to collect the biologic material after it has been disrupted, a blade-type rotor rotating in the internal chamber, adapted to cooperate, when rotating, with the fixed disrupting grid, in order to supply and take the biologic material from the upper loading chamber in contact with the microcells of the disrupting grid and thereby generate the disruption of the biologic material through these micro-holes.
  • the microcells of the disrupting grid have sizes or a diameter included between 70 and 80 ⁇ m and are obtained through a molding process of the bit type, using a conical or polygonal punch to draw and distort a metal sheet made of stainless steel, or through a laser light source, in order to make an edge with different cutters, for example six.
  • the configuration of the microcells allows operating without the help of chemical reagents, to obtain cellular suspensions and micro-grafts of tissues, keeping the characteristics and the functions of the original, non-disrupted biologic material intact and unaltered, and therefore can be advantageously used in several medical, veterinary and cosmetic applications.
  • the disrupting device solves the problem of the construction easiness, through the disposable device and the definition of the single internal chamber, but does not solve the problem of the axial load pressing on the axial bearing of the rotor, axial load following the necessary action to be able to disrupt the biologic substances.
  • the disrupting device according to WO-A2-2016097960 solves the problem of the disruption efficiency of the biologic cells, through the definition of the two chambers for loading biologic substances to be disrupted and for collecting disrupted biologic substances, but does not solve the problem of the axial load pressing on the axial bearing of the rotor.
  • a disrupting device for biologic substances according the preamble of claim 1 is disclosed in SU1637879A1 .
  • US-A1-2010/112696 discloses a disruptor according to the preamble of Claim 1.
  • object of the present invention is solving the above prior art problems, by providing a disruptor which allows the efficient disruption of the biologic substances, through the definition of two chambers for loading biologic substances to be disrupted and for collecting disrupted biologic substances, but at the same time removing the axial load of the rotor.
  • a further object of the present invention is providing a disruptor of biologic substances which allows keeping the advantages of a disposable solution and simplifying the assembling of parts composing a device chosen to satisfy the above problems.
  • the disrupting device 1 of biologic substances comprises:
  • the loading chamber and the collecting chamber are coaxial with respect to the rotation axis of the rotor 30 and, in particular, the collecting chamber is defined by the volume of the cylindrical crown interposed between the fixed disrupting grid 20 with a substantially cylindrical shape placed inside the internal chamber and the internal wall of the external hollow body 10, while the loading chamber is defined by the internal volume of the fixed disrupting grid 20 itself.
  • the fixed disrupting grid 20 is composed of a case with passages, of a substantially axial-symmetrical shape with respect to the rotor 30, to delimit the loading chamber of biologic substances to be disrupted around the rotation axis of the rotor 30 and the collecting chamber of disrupted biologic substances between the fixed disrupting grid 20 and the external hollow body 10.
  • the rotor 30 comprises at least one blade 31, substantially radially projecting with respect to the rotation axis of the rotor 30, and which extends substantially for the whole height of the fixed disrupting grid 20 to be able to take the biologic substances in contact with the fixed disrupting grid 20.
  • the blade 31 comprises at least one convex surface 2 with tapered end 3, in contact with the biologic substances to be taken in contact with the fixed disrupting grid 20.
  • the fixed disrupting grid 20 comprises a distribution of holed micro-cells 21 equipped with cutting edges 4 around the rotation axis of the rotor 30.
  • the disrupting device 1 comprises at least one removable closing cover 40 of the external hollow body 10 withdrawable from a projecting shaft of the rotor 30 through at least one hole 41.
  • the fixed disrupting grid 20 is delimited respectively by at least one base ring 22 and by at least one head ring 23.
  • the fixed disrupting grid 20 is supported by the external hollow body 10 through the base ring 22 in at least one grooved seat 11 of a bottom 12 and through the head ring 23 joined to at least one annular flange 50 in a seat 13 next to the removable cover 40.
  • annular flange 50 is shaped to allow accessing only to the loading chamber of biologic substances to be disrupted through at least one central opening 5 and emptying the collecting chamber of disrupted biologic substances through at least one connecting sleeve 6.
  • the fixed disrupting grid 20 is composed of a cylindrical case with base/height ratio equal to about 1 ⁇ 3.
  • the rotor 30 is preferably of the two-blade type with arrangement of the blades at 180°, with an axial extension approximately equal to the axial extension of the disrupting grid 20, while the external hollow body 10 is axial-symmetrical shaped as a frustum of cone.
  • the disrupting device 1 of biologic substances is preferably of the type with vertical axis.
  • the disrupting device of biologic substances of the present invention allows obtaining the above preset objects.
  • the disruption of the biologic substances efficiently occurs, through a radial flow of the biologic substances, from the central loading chamber for biologic substances to be disrupted, to the collecting chamber of disrupted biologic substances.
  • the axial load of the rotor is removed, with the advantage that it is not necessary to provide for an axial bearing, since the only operative axial load is a balanced radial one, due to an axial- symmetrical shape of the components of the device.
  • the disrupting device of biologic substances is of the type with vertical axis, composed of components which can be easily replaced.
  • microcells composing the fixed disrupting grid allows operating without the help of chemical reagents, to obtain cellular suspensions and micro-grafts of tissues, keeping the characteristics and the functions of the original, non-disrupted biologic material intact and unaltered, allowing the use of the disruptor of the invention in several medical, veterinary and cosmetic applications.
  • the connecting sleeve fastened to the annular flange to allow emptying the collecting chamber of disrupted biologic substances can comprise a connection of the LEUR type.

Landscapes

  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (7)

  1. Dispositif (1) de désintégration de substance biologique, comprenant:
    - au moins un corps externe creux (10) définissant au moins une chambre interne;
    - au moins une grille fixe de désintégration (20) logée à l'intérieur de ladite chambre interne pour définir au moins une chambre de chargement de substances biologiques à désintégrer et au moins une chambre de collecte de substances biologiques désintégrées, ladite grille fixe de désintégration (20) étant constituée d'un boîtier avec des passages, de forme sensiblement à symétrie axiale par rapport à au moins un rotor (30), pour délimiter ladite chambre de chargement de substances biologiques à broyer autour d'un axe de rotation dudit rotor (30) et ladite chambre de collecte des désagrégés des substances biologiques entre ladite grille de désintégration fixe (20) et ledit corps externe creux (10); et
    - ledit au moins un rotor (30) tournant à l'intérieur de ladite chambre interne pour amener lesdites substances biologiques en contact avec ladite grille fixe de désintégration (20) et pour coopérer avec ladite grille fixe de désintégration (20) pour permettre une désintégration et un passage depuis ledit chargement chambre à ladite chambre de collecte desdites substances biologiques désintégrées à travers ladite grille de désintégration fixe (20);
    ledit rotor (30) comprend au moins une aube (31), sensiblement en saillie radiale par rapport audit axe de rotation dudit rotor (30), ladite aube (31) s'étendant sensiblement sur toute la hauteur de ladite grille de perturbation fixe (20) , ladite lame (31) étant conçue pour amener lesdites substances biologiques en contact avec ladite grille fixe de désagrégation (20),
    caractérisé en ce que ladite lame (31) comprend au moins une surface convexe (2) à extrémité effilée (3), en contact avec lesdites substances biologiques à mettre en contact avec ladite grille fixe de désagrégation (20); et
    par le fait que ladite grille de perturbation fixe (20) comporte une répartition de micro-alvéoles perforées (21), lesdites micro-alvéoles perforées (21) étant munies d'arêtes coupantes (4) autour dudit axe de rotation dudit rotor (30) .
  2. Dispositif (1) de désintégration de substances biologiques selon la revendication 1, caractérisé en ce qu'il comprend au moins un couvercle amovible (40) pour pouvoir obturer l'ouverture du corps extérieur creux (10), ledit couvercle amovible (40) étant amovible d'un arbre en saillie dudit rotor (30) à travers au moins un trou (41).
  3. Dispositif (1) de désintégration de substances biologiques selon la revendication précédente, caractérisé en ce que ladite grille fixe de désagrégation (20) est délimitée respectivement par au moins un anneau de base (22) et par au moins un anneau de tête (23), ledit une grille de perturbation fixe (20) étant supportée par ledit corps extérieur creux (10) au moyen dudit anneau de base (22) dans au moins un siège rainuré (11) d'un fond (12) et au moyen dudit anneau de tête (23) reliée à au moins une bride annulaire (50) dans un siège (13) proche dudit couvercle amovible (40).
  4. Dispositif (1) de désintégration de substances biologiques selon la revendication précédente, caractérisé en ce que ladite collerette annulaire (50) est conformée pour n'autoriser l'accès qu'à ladite chambre de chargement de substances biologiques à désintégrer par au moins une ouverture centrale (5) et l'évacuation de la chambre de collecte des substances biologiques désintégrées à travers au moins un manchon de raccordement (6).
  5. Dispositif (1) de désintégration de substances biologiques selon l'une quelconque des revendications précédentes, caractérisé en ce que ladite grille fixe de désintégration (20) est constituée d'au moins une enveloppe cylindrique de rapport base/hauteur égal à environ ⅓, ledit rotor (30) étant du type bipale avec une disposition des pales à 180°, d'extension axiale sensiblement égale à l'extension axiale de ladite grille de perturbation (20).
  6. Dispositif (1) de désintégration de substances biologiques selon la revendication précédente, caractérisé en ce que ledit corps extérieur creux (10) est à symétrie axiale en forme de tronc de cône.
  7. Dispositif (1) de désintégration de substances biologiques selon l'une quelconque des revendications précédentes, caractérisé en ce qu'il est du type à axe vertical.
EP18000339.4A 2017-06-01 2018-04-10 Dispositif de perturbation de substances biologiques Active EP3409369B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000059930A IT201700059930A1 (it) 2017-06-01 2017-06-01 Dispositivo disgregatore di sostanze biologiche.

Publications (2)

Publication Number Publication Date
EP3409369A1 EP3409369A1 (fr) 2018-12-05
EP3409369B1 true EP3409369B1 (fr) 2023-06-14

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EP18000339.4A Active EP3409369B1 (fr) 2017-06-01 2018-04-10 Dispositif de perturbation de substances biologiques

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EP (1) EP3409369B1 (fr)
IT (1) IT201700059930A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11033295B2 (en) 2019-05-06 2021-06-15 Tissuemill Technologies Llc Atraumatically formed tissue composition, devices and methods of preparation and treatment

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878846A (en) * 1953-12-08 1959-03-24 Toledo Scale Corp Centrifugal forced meat chopping machine
US2886254A (en) * 1957-09-03 1959-05-12 Rohlinger Demountable and separable pulverizer
SU1637879A1 (ru) 1989-01-12 1991-03-30 Пензенский филиал Всесоюзного научно-исследовательского института антибиотиков Измельчитель биологических тканей
DE3941836A1 (de) * 1989-03-03 1990-09-06 Palmer Gmbh Maschf Reibeeinrichtung
US20100112696A1 (en) * 2008-11-03 2010-05-06 Baxter International Inc. Apparatus And Methods For Processing Tissue To Release Cells
WO2015035221A1 (fr) * 2013-09-05 2015-03-12 The Gid Group, Inc. Appareil de traitement de tissu et procédé de traitement de tissu adipeux

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EP3409369A1 (fr) 2018-12-05
IT201700059930A1 (it) 2017-09-01

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