EP3280249A1 - Supports pour cultiver un organisme, utilisations desdits supports et méthodes de culture et d'épuration utilisant ces supports - Google Patents
Supports pour cultiver un organisme, utilisations desdits supports et méthodes de culture et d'épuration utilisant ces supportsInfo
- Publication number
- EP3280249A1 EP3280249A1 EP16712231.6A EP16712231A EP3280249A1 EP 3280249 A1 EP3280249 A1 EP 3280249A1 EP 16712231 A EP16712231 A EP 16712231A EP 3280249 A1 EP3280249 A1 EP 3280249A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- porous
- layer
- porous support
- water
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/022—Pots for vertical horticulture
- A01G9/025—Containers and elements for greening walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Definitions
- the present invention relates to the cultivation of plant organisms, fungi or microorganisms (yeasts, bacteria).
- the invention relates to a porous support for cultivating an organism comprising two walls for receiving water, the two walls being arranged to form a receiving pocket of the body.
- Patent FR 2 960 384 describes such a support.
- the walls are interconnected at the top and bottom of the support, on the one hand, and these walls are impermeable to water, on the other hand, so that the water is retained in the support and stagnates therein. .
- the supply of oxygen is thwarted.
- the walls of the support of this prior art must be generally in a rot-proof material.
- the invention of the present application aims to overcome these disadvantages.
- each of the two walls comprises at least one layer of a chemically inert and porous material allowing water to flow through the pores of the layer, the porous layer being arranged to allow the body to to grip it.
- said layers of inert material, porous and gripping the two walls are internal layers.
- the root system of the organism can well develop, as the populations of fungi or microorganisms, useful for growing plants by facilitating the capture of nutrients, especially those provided by fertilizers organic, while the support of the prior art referred to above only tolerates chemical fertilizers.
- the roots of the organisms are better nourished, on the one hand, and better fixed. , on the other hand.
- GB 2 454 678 discloses a plant propagation device which comprises plastic material pouches having an aperture at the apex and openings at the base for draining the irrigation water, thereby avoiding the excesses of fluid.
- a plant propagation device which comprises plastic material pouches having an aperture at the apex and openings at the base for draining the irrigation water, thereby avoiding the excesses of fluid.
- Such a device can be mounted on a vertical wall.
- WO 2008/043904 describes modular wall elements for plant cultivation comprising plant attachment means and a gravitational flow vector of an aqueous nutrient solution of plants in the form of a tablecloth. lining a support plate.
- This sheet consists of a first water retention sheet capable of allowing the rooting of the plants and a second draining sheet.
- the present application also relates to the intermediate product of the above support consisting of a draining support for cultivating an organism comprising a single wall for receiving water, characterized in that the wall comprises at least one porous layer of a chemically inert material allowing water to flow through the pores of the layer, the porous layer being arranged to allow the body to grip it.
- the porous and gripper layers contain biodegradable fibers, intended to initialize colonies of microorganisms before the development of the roots of the body, such as, for example, hemp or coconut fibers.
- each wall comprises a porous and draining outer layer of protection and, preferably, a third porous interlayer and draining structural support of the wall.
- the supports of the present invention can be irrigated both continuously and intermittently, the problem of water saturation not being posed, thanks to the porosity.
- the invention also relates to a method of culturing aerobic bacteria and / or aerobic fungi comprising:
- a culture medium comprising water and organic nutrients
- a method for purifying a composition comprising organic compounds from biological waste comprising: to prepare a porous support according to what is described above, to seed this porous support with aerobic bacteria and / or aerobic fungi and / or plants, and
- FIG. 1 shows a cross-sectional view of the double-walled culture medium of the invention
- FIG. 2 schematically represents a front view of the support of FIG.
- FIG. 3 shows a cross section of the single-walled culture support of the invention.
- the culture support 5 of an organism 1 here a plant provided with leaves and flowers, comprises two walls 4, 4 'facing each other, each wall comprising a porous inner layer 1 comprising chemically inert fibers and here some biodegradable fibers 10, each wall here comprises an outer protective layer 3 and an intermediate layer consisting of chemically inert fibers 2 porous.
- the support 5 is fixed against a wall 7 by one of its walls 4 ', by conventional means perfectly within the reach of those skilled in the art.
- the other wall 4 of the support 5, opposite on a wall, 7, has been cut on a horizontal portion 21 in order to be able to spread part of it so as to form a pocket 31 for receiving the plant 11.
- the irrigation device 9 there is provided, above the pocket 31, an irrigation device 9 and, below the pocket 31, a collection container for drained water.
- the water of the irrigation device 9 flows along the support 5 by gravity through the pores of the layers of the walls 4, 4 '.
- the irrigation system can be a drip or an intermittent system. It will be noted that it is for simplicity and clarity that a support 5 with a single pocket 31 has been described. It is naturally easy to provide a support with a plurality or even a large plurality of culture pockets. It is used here a totally draining culture medium, that is to say whose water retention properties are very low or zero. This allows a better development of the root system 12 of the plant 1 1 in the draining support 5. This, combined with the presence of the pores of the walls 4, 4 'of the support 5, ensures a better mechanical support.
- biodegradable fibers 10 in the draining layers 1 of the porous support 5 Due to the low proportion of biodegradable fibers 10 in the draining layers 1 of the porous support 5, here slowly biodegradable fibers such as hemp or coconut fibers, better colonization by fungi and / or microorganisms is obtained by particular fungi and / or aerobic microorganisms, which has a utility in itself for the culture of these fungi and / or microorganisms, and improves the metabolism of roots 12 of the crop 1 1.
- hemp or coconut fibers Due to the low proportion of biodegradable fibers 10 in the draining layers 1 of the porous support 5, here slowly biodegradable fibers such as hemp or coconut fibers, better colonization by fungi and / or microorganisms is obtained by particular fungi and / or aerobic microorganisms, which has a utility in itself for the culture of these fungi and / or microorganisms, and improves the metabolism of roots 12 of the crop 1 1.
- Organic fertilizers here derived from vegetable waste, other than the waste of the cultivated plant
- the support allowing the presence of fungi and / or aerobic micro-organisms.
- the porous inner porous layer 1 is here essentially made from synthetic fibers, such as polyolefins, in particular polypropylene.
- the fibers constituting the porous and draining inner layer 1 are, for the most part, not biodegradable and are chemically inert, at least under the conditions of temperature and pH at which the organisms are cultured. These fibers chemically resist the metabolites secreted by the culture or the substances present in the fertilizers. In addition, these fibers do not provide nutrients to the cultured organisms. However, these fibers serve as physical support for the cultivation of these cultured microorganisms.
- the porous and draining inner layer (1) here comprises a small proportion, between 2 and 5% or 10%, of a biodegradable fiber, preferably slowly biodegradable, such as hemp or coconut fiber.
- a biodegradable fiber preferably slowly biodegradable, such as hemp or coconut fiber.
- Slowly biodegradable fiber means fibers that are totally degraded by organisms in the medium (eg the plant, the fungus and / or the microorganism grown, and / or ambient or added micro-organisms) in addition 10 months, preferably in more than 20 months, but preferably also in less than 60 months. The presence of these fibers increases the biomass of fungi and / or microorganisms in the porous support of the invention.
- the fibers forming the porous draining layers 1 and 2 of the walls 4 and 4 'of the support 5 may be woven or knitted, or else form a nonwoven fabric and / or a network of stitches. These layers 1 and 2 therefore have a certain thickness and internal porosity; their structure is continuous imperforate. Thus, even if the porous draining layers 1 and 2 are made from polyolefins, they differ from a perforated plastic made from polyolefins; their thickness and their porosity allow the roots 12 of the plant to grip in and / or between the porous draining layers 1 and 2.
- the layers 1 and 2 produced contain a large number of voids. These pores allow water and air to circulate. Thus, these layers 1 and 2 are not, or very little, hygroscopic.
- the porous, draining support of the present application is good for the culture of an organism, including a plant organism, because it contains significantly more atmospheric oxygen and allows for better colonization by the root system, as well as colonization by fungi and / or microorganisms.
- This colonization does not prevent the drainage of irrigation water, even over time, that is to say that there remains a significant proportion of pores that are not obstructed by this colonization.
- a vertical support was considered. It can also be tilted to the vertical so that the irrigation water flows by gravity.
- the culture support 6 has only one wall 14.
- the wall 14 is substantially horizontal. It comprises an upper layer 41 which is porous, mainly inert, and draining. Its characteristics are identical to those of the inner layer 1 of the support 5 of Figures 1 and 2, with the same reserves and the same options.
- the wall 14 here comprises a lower layer 42, with the same characteristics and options as the intermediate layer 2 of the support 5 of FIGS. 1 and 2.
- the support 6, on the other hand, does not have a bag for receiving the organism, but the same organisms can be cultivated thereon as on the support 5 with two walls. It is associated with a support device shown here on trestles 45 and collection 46 irrigation water. The irrigation system is not shown in Figure 3.
- the present invention allows the cultivation of fungi and / or aerobic microorganisms.
- This cultivation of mushrooms and / or aerobic microorganisms can in practice be a co-culture with plants, which allows a symbiosis at the roots of the plants (the plant takes advantage of the fungus and / or the microorganism, and vice versa) or to modify the secondary metabolism of the plant, or even the fungus and / or microorganism, to optimize the production of desired metabolites.
- the biological waste treatment method consists in preparing a culture support with one or two walls, such as, preferably a culture support containing the porous layer comprising biodegradable fibers, to seed this support with plants and / or fungi (aerobic) and / or microorganisms (aerobes), and to irrigate this medium inoculated with a biological waste.
- This waste which may comprise one or more of peptides and / or metabolites thereof, nucleic acids and / or metabolites thereof, polysaccharides and / or metabolites thereof, should be compatible with the metabolism of organisms grown on this support, or made compatible with the metabolism of cultured organisms.
- the biological waste to be purified may be diluted or mixed with other biological wastes and / or other nutrients so that its different components correspond to the metabolism of the cultivated species (content of nitrogen compounds, sugar content, mineral content ).
- the possible presence of toxic compounds for the (co-) culture should be controlled, and if necessary adapted (selective extraction, dilution of the composition, adjustment of the pH).
- Those skilled in the art are able to adapt the flow of waste so as to maintain the (co-) culture over time while eliminating a maximum of waste (biological) because of the metabolism of the cultured organisms.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Virology (AREA)
- Immunology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Hydroponics (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE2015/0116A BE1023546B1 (fr) | 2015-04-07 | 2015-04-07 | Supports pour cultiver un organisme, utilisations desdits supports et methodes de culture et d'epuration utilsant ces supports |
PCT/EP2016/054710 WO2016162152A1 (fr) | 2015-04-07 | 2016-03-04 | Supports pour cultiver un organisme, utilisations desdits supports et méthodes de culture et d'épuration utilisant ces supports |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3280249A1 true EP3280249A1 (fr) | 2018-02-14 |
Family
ID=53800774
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP16712231.6A Withdrawn EP3280249A1 (fr) | 2015-04-07 | 2016-03-04 | Supports pour cultiver un organisme, utilisations desdits supports et méthodes de culture et d'épuration utilisant ces supports |
Country Status (7)
Country | Link |
---|---|
US (1) | US20180077877A1 (fr) |
EP (1) | EP3280249A1 (fr) |
CN (1) | CN107683331A (fr) |
BE (1) | BE1023546B1 (fr) |
CA (1) | CA2961734C (fr) |
RU (1) | RU2017137747A (fr) |
WO (1) | WO2016162152A1 (fr) |
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RU199609U1 (ru) * | 2020-05-20 | 2020-09-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU200507U1 (ru) * | 2020-05-20 | 2020-10-27 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU199592U1 (ru) * | 2020-05-20 | 2020-09-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU199593U1 (ru) * | 2020-05-20 | 2020-09-09 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU199790U1 (ru) * | 2020-05-20 | 2020-09-21 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU199792U1 (ru) * | 2020-05-20 | 2020-09-21 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
RU199793U1 (ru) * | 2020-05-20 | 2020-09-21 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный технический университет" (ВолгГТУ) | Модуль для выращивания растений на гидропонике |
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CN102907274A (zh) * | 2012-10-10 | 2013-02-06 | 张宇顺 | 立体绿化结构组件及立体绿化方法 |
FR2998292B1 (fr) * | 2012-11-19 | 2016-07-08 | Epur Nature | Dispositif de traitement biologique des eaux usees |
CN203096694U (zh) * | 2013-02-27 | 2013-07-31 | 河海大学 | 一种吸附型生态护坡砌块 |
CN203446267U (zh) * | 2013-07-05 | 2014-02-26 | 深圳市铁汉生态环境股份有限公司 | 一种垂直绿化种植容器 |
CN103650966B (zh) * | 2013-12-10 | 2016-03-16 | 北京东方园林股份有限公司 | 一种模块化垂直绿化装置及其制造方法 |
CN203864311U (zh) * | 2014-04-18 | 2014-10-08 | 吴德煌 | 植物画框 |
-
2015
- 2015-04-07 BE BE2015/0116A patent/BE1023546B1/fr not_active IP Right Cessation
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2016
- 2016-03-04 EP EP16712231.6A patent/EP3280249A1/fr not_active Withdrawn
- 2016-03-04 RU RU2017137747A patent/RU2017137747A/ru not_active Application Discontinuation
- 2016-03-04 US US15/564,477 patent/US20180077877A1/en not_active Abandoned
- 2016-03-04 WO PCT/EP2016/054710 patent/WO2016162152A1/fr active Application Filing
- 2016-03-04 CA CA2961734A patent/CA2961734C/fr not_active Expired - Fee Related
- 2016-03-04 CN CN201680025905.1A patent/CN107683331A/zh not_active Withdrawn
Also Published As
Publication number | Publication date |
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BE1023546A1 (fr) | 2017-04-28 |
US20180077877A1 (en) | 2018-03-22 |
CN107683331A (zh) | 2018-02-09 |
BE1023546B1 (fr) | 2017-04-28 |
RU2017137747A (ru) | 2019-05-07 |
WO2016162152A1 (fr) | 2016-10-13 |
CA2961734C (fr) | 2020-05-12 |
CA2961734A1 (fr) | 2016-10-13 |
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