EP3102677A4 - Genomic dna extraction reagent and method - Google Patents

Genomic dna extraction reagent and method Download PDF

Info

Publication number
EP3102677A4
EP3102677A4 EP14881909.7A EP14881909A EP3102677A4 EP 3102677 A4 EP3102677 A4 EP 3102677A4 EP 14881909 A EP14881909 A EP 14881909A EP 3102677 A4 EP3102677 A4 EP 3102677A4
Authority
EP
European Patent Office
Prior art keywords
genomic dna
dna extraction
extraction reagent
reagent
genomic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14881909.7A
Other languages
German (de)
French (fr)
Other versions
EP3102677A1 (en
Inventor
Yanshan Ji
Xiaoyin Fei
Wenjin Yu
Volker Mittendorf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Participations AG
Original Assignee
Syngenta Participations AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/171,816 external-priority patent/US9556427B2/en
Priority claimed from US14/211,113 external-priority patent/US20140242584A1/en
Application filed by Syngenta Participations AG filed Critical Syngenta Participations AG
Publication of EP3102677A1 publication Critical patent/EP3102677A1/en
Publication of EP3102677A4 publication Critical patent/EP3102677A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP14881909.7A 2014-02-04 2014-12-23 Genomic dna extraction reagent and method Withdrawn EP3102677A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/171,816 US9556427B2 (en) 2013-02-27 2014-02-04 Methods and compositions for preparation of nucleic acids
US14/211,113 US20140242584A1 (en) 2013-02-27 2014-03-14 Genomic dna extraction reagent and method
PCT/US2014/072151 WO2015119719A1 (en) 2014-02-04 2014-12-23 Genomic dna extraction reagent and method

Publications (2)

Publication Number Publication Date
EP3102677A1 EP3102677A1 (en) 2016-12-14
EP3102677A4 true EP3102677A4 (en) 2017-06-28

Family

ID=53778331

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14881909.7A Withdrawn EP3102677A4 (en) 2014-02-04 2014-12-23 Genomic dna extraction reagent and method

Country Status (4)

Country Link
EP (1) EP3102677A4 (en)
AU (1) AU2014381626A1 (en)
CA (1) CA2901406A1 (en)
WO (1) WO2015119719A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109628313A (en) * 2019-01-24 2019-04-16 长春万成生物电子工程有限公司 A kind of E. coli lysate and its preparation method and application

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109306349A (en) * 2017-07-26 2019-02-05 天津迦美惠众科技有限公司 A kind of biological sample nucleic acid extraction purification process based on nanometer magnetic bead
AU2018350883A1 (en) * 2017-10-16 2020-04-23 Syngenta Participations Ag High-throughput method of for mitochondria isolation from plant seeds
CN109371012B (en) * 2018-11-27 2021-02-09 杭州安誉新桥教育科技有限公司 Lysate and method for extracting meat nucleic acid
CN110484533A (en) * 2019-09-05 2019-11-22 毕节市农业科学研究所 A kind of preparation method of the rice leaf largely extracted for DNA
CZ309658B6 (en) * 2021-02-08 2023-06-21 Bioinova, A.S. Solution for molecular diagnosis of viral infections by PCR and collection kit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014018195A1 (en) * 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2833273B1 (en) * 2001-12-07 2004-08-13 Commissariat Energie Atomique METHOD FOR THE DETECTION AND QUANTITATIVE ANALYSIS OF NUCLEIC ACID MOLECULES COMPRISING A STRONG BASED EXTRACTION STAGE AND APPLICATIONS
US7727718B2 (en) * 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
WO2009111336A2 (en) * 2008-02-29 2009-09-11 Shizhong Chen Methods of purifying plasmid dna
US20140242584A1 (en) * 2013-02-27 2014-08-28 Syngenta Participations Ag Genomic dna extraction reagent and method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014018195A1 (en) * 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GENIAL - GENTECHNOLOGIE IN AUSBILDUNG UND LABOR: "Laboratory Protocols", 3 September 2009 (2009-09-03), pages 1 - 7, XP055373235, Retrieved from the Internet <URL:https://www.researchgate.net/file.PostFileLoader.html?id=540520d9d3df3ea9398b4599&assetKey=AS:273590912520213@1442240565019> [retrieved on 20170516] *
JINGADE H ANURADHA ET AL: "A novel and efficient protocol for the isolation of genomic DNA from mulberry (Morus L.)", EMIRATES JOURNAL OF FOOD AND AGRICULTURE, vol. 25, no. 2, 28 November 2012 (2012-11-28), pages 124 - 131, XP055219755, ISSN: 2079-052X, DOI: 10.9755/ejfa.v25i2.11660 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109628313A (en) * 2019-01-24 2019-04-16 长春万成生物电子工程有限公司 A kind of E. coli lysate and its preparation method and application

Also Published As

Publication number Publication date
EP3102677A1 (en) 2016-12-14
CA2901406A1 (en) 2015-08-13
WO2015119719A1 (en) 2015-08-13
AU2014381626A1 (en) 2015-09-10

Similar Documents

Publication Publication Date Title
EP3332034A4 (en) Systems and methods for genomic analysis
HK1223651A1 (en) Rapid nucleic acid extraction method and apparatus
HK1223972A1 (en) Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dna
EP3132025A4 (en) Methods and compositions for modifying genomic dna
EP3271848A4 (en) Systems and methods for analyzing nucleic acid
DK3604544T3 (en) Improved methods for processing dna substrates
EP3095054A4 (en) Methods and systems for genome analysis
EP3450569A4 (en) Dna amplification method
EP3164510A4 (en) Method, substrate and device for separating nucleic acids
EP3478417A4 (en) Devices and methods for nucleic acid extraction
EP3194627A4 (en) Methods and systems for analyzing nucleic acid sequencing data
HK1250758A1 (en) Method and reagent for preparing circular single-stranded nucleic acid library
EP3115959A4 (en) Data analysis system and method
EP3096149A4 (en) Soft stopper-penetrating pipetting device, and soft stopper-penetrating pipetting method
SG10201600867XA (en) Nucleic acid extracting device
EP3214164A4 (en) Microfluidic device for detecting target gene, method for manufacturing same, and method for detecting using same
EP3460072A4 (en) Nucleic acid extraction method using solid subject
EP3102677A4 (en) Genomic dna extraction reagent and method
EP3508587A4 (en) Dna sequencing method
EP3491003A4 (en) Device and method for nucleic acid manipulation
EP3234206A4 (en) Heap leaching method
EP3189160A4 (en) Method and device for polymerase chain reaction
EP3202901A4 (en) Method for nucleic acid amplification
EP3103884A4 (en) Nucleic acid detection and assay method using mask oligonucleotide, and device for same
EP3199615A4 (en) Nucleic acid analysis device

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20160905

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20170531

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/10 20060101AFI20170524BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20180103