EP3095846A1 - Reinigungsmittel für oberflächen, wie fussböden, glaswände, objekte und dergleichen, und ein verfahren zur reinigung solcher oberflächen - Google Patents
Reinigungsmittel für oberflächen, wie fussböden, glaswände, objekte und dergleichen, und ein verfahren zur reinigung solcher oberflächen Download PDFInfo
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- EP3095846A1 EP3095846A1 EP16163756.6A EP16163756A EP3095846A1 EP 3095846 A1 EP3095846 A1 EP 3095846A1 EP 16163756 A EP16163756 A EP 16163756A EP 3095846 A1 EP3095846 A1 EP 3095846A1
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- enzyme
- cleaning
- preparation
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- cleaning preparation
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/14—Hard surfaces
Definitions
- the present invention relates to a cleaning preparation for surfaces such as floors, walls, windows, objects and the like, and to a method for cleaning such surfaces.
- the activities for cleaning surfaces such as floors, walls, windows, objects and the like and/or the layering that may possibly be present on such surfaces in general, provide for the use of cleaning products and/or disinfectants of various types, which are distributed over the surface to be cleaned and/or on the layers present on such a surface to remove both organic (fats, proteins, sugars, etc.) and inorganic substances that generally make up the accumulations of dirt, as well as to eliminate any bacterial contamination formed on the surface itself (and which typically proliferates on the above accumulations of dirt).
- cleaning products and/or disinfectants of various types which are distributed over the surface to be cleaned and/or on the layers present on such a surface to remove both organic (fats, proteins, sugars, etc.) and inorganic substances that generally make up the accumulations of dirt, as well as to eliminate any bacterial contamination formed on the surface itself (and which typically proliferates on the above accumulations of dirt).
- the cleaning systems applied today provide, in most cases, at least three daily applications of cleaning preparations with a high content of surfactants and/or molecules having a detergent and/or disinfectant effect.
- This frequency of application is due to the fact that, subsequently to the cleaning action, the organic and/or inorganic substances mainly forming the dirt and the consequent growth of pathogens harmful to health from the external environment begin to contaminate the same surfaces again.
- the cleaning agents and disinfectants normally used require the rinsing step, since their action is simply to make the substances forming the dirt water-soluble, and do not show any chemical-physical feature that may degrade (i.e. break) the molecules forming the dirt.
- the technical task of the present invention therefore is to improve the prior art.
- an object of the present invention is to solve the drawbacks described above by providing a cleaning preparation which allows reducing the number of operations required to obtain the optimum cleaning of the surface of interest.
- Another object of the present invention is to provide a cleaning preparation which allows reducing the consumption of materials, in particular water and chemical substances, at least having the same results obtained on the surfaces concerned.
- a further object of the invention is to provide a cleaning preparation which ensures high reliability of operation over time on the surfaces on which it is applied and/or on the layers present on such surfaces.
- Yet another object of the present invention is to provide a cleaning preparation which is cost-effective and safely applied.
- a further object of the present invention is to provide a cleaning preparation which is easily obtainable starting from elements and materials commonly commercially available.
- another object of the present invention is to also develop a method for cleaning surfaces such as floors, walls, windows, objects and the like which allows reducing the number of manual and/or mechanical interventions, for the temporary removal of dirt and of the resulting proliferation of agents potentially harmful to health.
- the cleaning preparation according to the invention comprises at least one enzyme responsible for the degradation of organic substances which can be anchored to the surface to be cleaned and/or to the layers present on the surface to be cleaned, and at least one immobilizing agent for immobilizing and maintaining the activity of said enzyme over the time subsequent to its anchoring to the surface and/or to the layers present on said surface, so as to prolong the degradation activity of the organic substances.
- Such a degradation is able to reduce the contaminating substances directly on the surface and/or on the layers present on that surface.
- the cleaning method according to the present invention comprises a step of applying, on the surface to be cleaned or on layers present on such a surface to be cleaned, a cleaning preparation or a solution comprising at least one enzyme responsible for the degradation of organic substances.
- the process also comprises a step of applying, on the surface to be cleaned or on the layers present on such a surface, at least one immobilizing agent for immobilizing and maintaining the activity of said enzyme over the time subsequent to its anchoring to the surface and/or to layers present on such a surface, so as to prolong the degradation activity of the organic substances.
- the cleaning preparation is applied, according to the invention, through materials consisting of polymers of a different nature.
- such polymers may be polyamides, polyethylene, polypropylene, polylactates, polymers with a high degree of bio-decomposition, etc. Subsequently, for a period of time that can reach 42 days, unlike the current cleaning methods, the manual cleaning operations can be avoided or significantly reduced anyway, due to the ability of the cleaning preparation to perform its activity of degradation of dirt which accumulates over the time subsequent to the application and/or cleaning step.
- the cleaning method according to the invention can include, in a non-exclusive manner, a cleaning operation with the cleaning preparation object of the invention, through the standard techniques.
- the enzyme and/or enzymes contained in the preparation remain anchored to the surface to be cleaned and/or to the layers present on said treated surface for an extended period of time (5 hours to 42 days).
- the degradation efficacy of the preparation against the organic and/or inorganic substances mainly forming the dirt is maintained over time, thus allowing a reduction in the accumulation of such substances due to the environment and to the persons who spend time in or contaminate such surfaces.
- formulations containing surfactants may have a negative effect on the catalytic activity in solution, so as to deeply inhibit the immobilization on surfaces.
- one of the advantages of the invention consists in the stabilization of the enzyme anchoring to the surface to be cleaned and/or to the stratified substances present thereon, and the enzyme efficacy is maintained for more than 7 days.
- Another peculiarity of the invention consists in the fact that the preparation described above is able to prevent the harmful phenomena arising from the accumulation of the substances making up the dirt, thus having a prolonged efficacy over time.
- the cleaning preparation according to the invention is particularly, but not exclusively, intended for use on surfaces to be cleaned such as floors or walls (for example made of PVC, linoleum or tile), windows, objects and the like (although not excluding the use, still falling within the scope of protection claimed herein, for cleaning other elements, depending on specific requirements).
- the cleaning preparation comprises at least one enzyme which can be stably anchored to the surface to be cleaned and/or to the layers present on said surface, and which is responsible for the degradation of organic substances, such as fats, proteins and/or sugars, and inorganic substances such as, by way of non-limiting example, carbonates salt powders.
- organic substances such as fats, proteins and/or sugars
- inorganic substances such as, by way of non-limiting example, carbonates salt powders.
- the enzyme may thus stably reduce or eliminate the accumulation of dirt present on the surface and/or on the layers present on such a surface at the time of the application of the preparation (and precisely consisting of the organic and/or inorganic substances mentioned above), since it allows the degradation thereof and promotes the solubility thereof, thereby allowing the removal thereof with a possible subsequent rinsing and/or slight mechanical action.
- the enzyme since the enzyme remains stably anchored to the surface and/or to the layers present on such a surface over time, it is able to exert its degradation activity even at a later time, for a more or less prolonged period (even several days) subsequent to the time of application of the preparation, and without the contribution of manual and/or mechanical operations by means of systems external to the same surface and/or to the layers present on such a surface for the whole period indicated above, thereby achieving the object set.
- the degradation activity of the enzyme towards the substances that make up the dirt over time also allows controlling the development of pathogens harmful to health, and the consequent reduction of their amount compared to the normal proliferation which occurs as a result of the accumulation of such substances after the conventional cleaning operation.
- the latter comprises one or more substances which make the immobilization of the enzyme itself effective, which will be defined as immobilizing agents hereinafter.
- immobilizing agents allow immobilizing the enzyme without altering the efficacy (activity) over time, in order, precisely, to prolong the activity of degradation of organic substances, subsequent to its anchoring to the surface and/or layers present on said surface.
- This solution allows avoiding the danger of loss of efficacy of the enzyme that could otherwise occur, for example, due to exposure to atmospheric agents or in any case to the external environment or through the mechanical rubbing to which the surfaces treated are subjected as a result of the normal use thereof.
- the immobilizing agents which maximize the efficacy (activity) of the enzyme in removing dirt include, or consist of, pH regulators and/or surfactants.
- the surfactants present in the preparation may be ionic and/or anionic and/or amphoteric and/or cationic and/or a combination thereof.
- they may be ionic and/or cationic surfactants.
- the enzyme Due to the components present in the preparation, and in particular due to the pH regulators, the enzyme is thus also able to withstand changes in pH, temperature, etc. over time, which might otherwise cause the deactivation thereof.
- concentrations of the enzymes and/or the other preparation components are such as to allow a degree of immobilization of more than 1% of the enzyme itself.
- the immobilizing agents include nonionic surfactants and/or cationic surfactants, or a combination thereof in a percentage not higher than 5% w/w of the preparation object of the invention.
- the enzyme immobilization may occur through the application of a further preparation or solution (containing no enzymes) comprising at least one immobilizing agent, with the aim, in fact, to immobilize the surfactants on the surface to be treated and/or on the layers present thereon by absorption and/or adsorption.
- the surfactants allow the enzyme and/or enzymes, through even weak chemical interactions, to be effectively immobilized, even in the presence of layers of substances present on the surfaces themselves.
- the enzyme can be stably anchored to the surface and/or to the layers present on said surface by absorption or by adsorption.
- the enzyme penetrates and spreads to the inner layers of the surface and/or layers present on such a surface to be cleaned.
- the enzymes form a chemical bond or establish a chemical-physical interaction, through Van der Waals forces, limited to the outer layers of the surface and/or layers present on such a surface.
- the anchoring of the enzyme occurs in a pH range of the cleaning preparation comprised between 6 and 8.5 and preferably between 6.5 and 8.5, and even more preferably between 7 and 8.
- pH ranges allow maximizing the immobilization yield of the enzyme itself, determining the maximum enzymatic activity of degradation of the organic and/or inorganic substances over time.
- anchoring and immobilization of the enzyme according to other methods is not excluded and falls within the scope of protection claimed herein.
- the anchoring methods described above have the feature, not obvious, to maintain the enzymatic activity over time (days) at sufficient levels and such as to degrade the component substances of dirt, resulting in controlling the growth of harmful organisms, and to be resistant to chemical contamination phenomena and/or mechanical actions exerted on the surface and/or the layers present on such a surface.
- the non-obvious particularity of the invention is the maintenance of the enzymatic activity over time, even in the presence of secondary components such as surfactants.
- surfactants may be a means of immobilization of the enzymes themselves, but at the same time they inhibit their activity both immediately and over time.
- the enzyme is selected from a protease, a lipase and an amylase, in order to be able to effectively degrade the organic substances such as proteins, fats and sugars, respectively, coming from sources external to the surface and/or to the layers present on such a surface to be cleaned.
- the enzymes may be sibtilisin and/or cellulase and/or lipase.
- concentrations of these enzymes and/or of the other additives in the preparation object of the invention is such as to obtain an immobilization yield greater than 10% on the surfaces and/or layers present on such surfaces.
- the surfactants present in the preparation allow increasing the wettability of the surface and/or the layers present on such a surface intended to be treated with the preparation.
- the preparation described above is applied on the surface and/or on the layers themselves so as to allow, in the first place, the enzyme contained therein to carry out its degradation activity on the organic substances, on the accumulations of dirt already present.
- the preparation is able to carry out a residual degradation activity which can last over time even for several days.
- the cleaning preparation is adapted to carry out the degradation function even after its application and without the need for subsequent manual and/or mechanical operations for a time of at least 1 day, preferably for at least 3 days, more preferably for at least 42 days.
- the preparation is adapted to control and reduce the proliferation of pathogens harmful to health on the surface to be cleaned and/or on the layers present on such a surface by at least 20%, preferably at least 30%, even more preferably at least 80%.
- Such degradation activity allows preventing the formation of new accumulations of dirt, also due to the immobilization efficiency ensured by the pH regulator components and by the surfactants, so as to impart greater resistance to the enzyme against external environmental factors, which could otherwise cause its deactivation.
- the absence of accumulations of organic substances means absence of the element essential for the proliferation of pathogens harmful to health, and therefore, over time, the surface and/or the layers present on such a surface are also protected from such pathogens.
- the preparation according to the invention thus ensures a prolonged cleaning over time without the need for continuous washing actions, resulting in an evident saving of product, in addition to that of water needed for rinsing (and possibly for the dilution of the preparation itself).
- the anchoring of the enzyme to the surface and/or to the layers present on such a surface is obtained without resorting to specific devices, such as polymeric films or coatings, thereby ensuring greater structural simplicity and a reduced number of necessary components, thus allowing a useful containment of the overall cost.
- the application examples described hereinafter relate to the treatment of floor surfaces, and specifically relate to three types of floors, i.e. single-fired, linoleum and PVC.
- a first aspect of the application examples described relates to the determination of optimal pH values, for each type of floor, in terms of enzyme immobilization.
- the amount of enzyme immobilized in mg/mL and in percentage was evaluated for each protein (enzyme) and each type of floor, and each pH value (in the range of 6.5 to 9).
- the enzymes used are, in particular, Lipolase®, Alcalase® and Savinase®.
- Table A, B, C refer to tests on single-fired tiles with an amount of enzyme used of 1 mg/mL, with pH between 6.5 and 9 and a duration of immobilization of 3h.
- Table A Alcalase® pH [Immobilized enzyme] (mg/ml) ⁇ % Immobilized e.
- Alcalase® shows a maximum absorption corresponding to about 20% of the total amount of enzyme placed in contact with the surface, while Savinase® is almost 40%.
- Lipolase® shows a contrary trend. It also shows no adsorption at basic pH, but the maximum immobilization is seen at pH 6 with nearly 60% of the total enzyme immobilized on the tiles.
- the immobilization is decreasing with an increasing pH, with about 35% of adsorption at a pH of 7.5, at the relative maximum of the protease.
- the data obtained also allow making some additional evaluations.
- the waxed surface of linoleum limits the amount of enzymes adsorbed on the tiles in absolute terms.
- Single-fired surfaces possess a good level of porosity and facilitate the enzyme adsorption even in the inner interstices of the tile, increasing the available surface.
- Both Savinase® and Alcalase® show a limited but significant level of non-specific adsorption at the so-called "extreme" pH of our considered range (6.5 and 9), associated with a visible decrease in the opacity of the floor.
- PVC is the surface that shows the most homogeneous trend, as a function of pH, as regards the enzyme immobilization.
- the first set of tests had a dual purpose:
- the second set of tests was conducted in duplicate and was aimed to confirm the activity of Lipolase® despite the small percentage of immobilization at pH 8 compared to both Savinase® and Alcalase®.
- the experimental evidences also comprise the "standard dirt" removal test for each type of material.
- each of the three tiles for each type was treated as follows:
- Tables K, L, M shown hereinafter and the graphs in figures 4 , 5, 6 show the trend of the dirt removal (in mg) from the surfaces with immobilized enzymes after 4 consecutive days of washes, with respect to both types of controls.
- Table K dirt removed (single-firing) Dirt removed in mg (single-firing)
- Samples Controls A Controls B Tris-HCl pH8, “Basic detergent” (3%) and enzyme mixture) (Tris-HCl pH 8, "Basic detergent” (3%)) (Tris-HCl pH 8) Days 1 (5.18 ⁇ 0.35) (2.53 ⁇ 0.45) (2.00 ⁇ 0.92) 2 (4.80 ⁇ 0.57) (2.80 ⁇ 0.22) (1.97 ⁇ 0.34) 3 (6.12 ⁇ 0.74) (4.33 ⁇ 0.45) (2.43 ⁇ 0.50) 4 (6.03 ⁇ 1.48) (3.30 ⁇ 0.92) (3.48 ⁇ 0.79)
- Table L dirt removed (Linoleum) Dirt
- the washes were carried out once a week for 42 days, and aliquots of 100 ⁇ l of the washing waters were transferred on Petri dishes containing agar LB medium (Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L, Agar 15 g/L).
- agar LB medium Teryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L, Agar 15 g/L.
- Table O bacterial counts at 14 days from the application of "standard dirt” Bacterial counts (14 days from the application of “standard dirt") Samples Controls A Controls B Dilutions M L P M L P M L P 10 1 12 1 2 0 4 5 0 296 31 10 0 14 0 1 0 0 1 0 5 2 10 -1 1 0 0 0 0 1 0 1 1 Table P: bacterial counts at 21 days from the application of "standard dirt” Bacterial counts (21 days from the application of "standard dirt") Samples Controls A Controls B Dilutions M L P M L P M L P 10 1 22 0 2 23 25 114 8 6 0 10 0 2 0 1 7 2 10 1 6 0 10 -1 0 0 1 0 1 6 3 0 0 Table Q: bacterial counts at 28 days from the application of "standard dirt” Bacterial counts (28 days from the application of "standard dirt”) Samples Controls A Controls B Dilutions M L P M L P M L P 10
- the washing water volume transferred on each Petri dish being known (100 ⁇ L), it is easily possible to derive the value of colony forming units (cfu) per milliliter of washing water from the number of colonies observed in each plate.
- control A and B which reach about 100 cfu/mL (control A - PVC), and even higher in the case of control B referred to linoleum.
- the porous and not homogeneous structure of this type of floor favors an increase in the total bacterial load, in addition to a lower protective effect by the enzymes. Even in this case, however, a decreasing trend of the microbial load may be seen in the tiles containing the Lipolase® Alcalase® and Savinase® mixture, reflecting the possible effect of reduction of the total microbial load values in the long run.
- the object of the present invention is also a method for cleaning surfaces such as floors, walls, windows, objects and the like.
- the method comprises at least a step of applying, on the surface to be cleaned or on layers present on such a surface to be cleaned, a solution comprising at least one enzyme responsible for the degradation of organic substances.
- the method further comprises a step of applying, on such a surface to be cleaned or on the layers present thereon, at least a further preparation or solution comprising an immobilizing agent for immobilizing and maintaining the activity of the enzyme over the time subsequent to its anchoring to the surface and/or to layers present on such a surface, so as to prolong the degradation activity of the organic substances.
- the step of applying a further preparation or solution comprising an immobilizing agent is carried out after the step of applying the cleaning preparation or solution comprising at least one enzyme.
- the step of applying a further preparation or solution comprising an immobilizing agent is carried out at the same time as the step of applying the cleaning preparation or solution comprising at least one enzyme.
- the method also comprises at least one step of applying, on the surface to be cleaned or on the layers present on such a surface to be cleaned, a second cleaning preparation, the latter without enzymes.
- such a second cleaning preparation is of the type with low content of surfactants.
- such a second cleaning preparation without surfactants can be of the type already known and available on the market.
- the step of applying the second cleaning preparation is subsequent to the step of applying the solution comprising at least one enzyme, i.e., in a preferred embodiment, the cleaning preparation according to the invention as described above.
- the method provides an alternation of steps of application of the cleaning preparation according to the invention and of application of the second cleaning preparation (without enzymes).
- the method may comprise, in some embodiments thereof, the alternating application of the two preparations (the one according to the invention and the second preparation without enzymes) at alternating days and/or weeks and/or months.
- the method provides a specific sequence of application steps of the cleaning preparation according to the invention, characterized by different modes of application in terms of frequency over time, as will become clearer hereinafter.
- the application of the second cleaning preparation can be significantly reduced in terms of amount and/or frequency of application.
- a specific embodiment relates to the light cleaning of small surfaces.
- the method provides a step of application of the cleaning preparation (of the invention) on the surfaces concerned using a cloth.
- Said cloth may be made of materials consisting of polymers of a different nature, such as polyamides, polyethylene, polypropylene, polylactates, polymers with a high bio-decomposition degree, etc.
- the purpose of such application/distribution of the cleaning preparation according to the invention is to make the surface treated by the enzyme uniform.
- the conventional cleaning procedures provide for more than three daily operations to maintain the degree of dirt, or of bacterial contamination (expressed in cfu/cm 2 ) to a value of less than 10.
- the cleaning preparation containing at least one enzyme is applied at most once a day for the first week.
- the method according to the invention provides for the application of the second cleaning preparation (without enzymes and with a low content of surfactants), for normal light cleaning operations, also at most once a day.
- the method again provides for the application of the cleaning preparation according to the invention, again - at most - once a day.
- the experimental data provided hereinafter - see in particular TEST 3 - show how the method according to the invention allows control of the bacterial load (and of the consequent effects harmful to the health of the areas, also as regards the unpleasant odors) reducing by at least 10%, preferably 20%, preferably 30% the times and the cleaning operations.
- the method according to the invention provides only one daily application of the product, also with a low content of detergent surfactants.
- the first step of the method consists in applying the cleaning preparation according to the invention (containing the enzyme to be immobilized) at most once a day for the first 7 days.
- This operational step is also compatible with a possible, but not necessary, light cleaning operation carried out with a traditional cleaning preparation low in surfactants and/or molecules with a detergent and/or disinfectant effect.
- the method provides a step of application of the preparation according to the invention for a maximum period of 3 days, with the same procedure described for the previous embodiment.
- the method provides a further step of application of the preparation according to the invention for a maximum period of 2 days, with the same procedure.
- This last step is further repeated weekly for a maximum period of three months.
- the application of the cleaning preparation is carried out according to a sequence of steps; each of these steps is characterized by a respective number of applications per week.
- the number of weekly applications is therefore progressively decreasing during at least the first three weeks from the beginning of the method or treatment.
- the cleaning operations intended to control the environmental dirt and/or the proliferation of pathogens may be carried out in a maximum number of two times daily, preferably once daily, preferably once every two days.
- the method provides the application of the cleaning preparation according to the invention at most once a week. Subsequently, the cleaning operations intended to control the environmental dirt and/or the proliferation of pathogens can be carried out at most twice daily, preferably once daily, preferably once every two days, by the use of cleaning preparations low in surfactants and/or molecules with a detergent and/or disinfectant effect (such as, but not exclusively, the second cleaning preparation described above).
- the step of applying the cleaning preparation according to the invention is carried out once every two days.
- the normal cleaning operations intended to control the environmental dirt and/or the proliferation of pathogens may be reduced to up to at most three times a day, preferably twice a day, preferably once a day, using cleaning preparations with a low content of surfactants and/or molecules with a detergent and/or disinfectant effect.
- the method provides a step of applying the cleaning preparation according to the invention at most once a day.
- the tests were conducted on areas and surfaces of health-care facilities, i.e. patient rooms, toilets, fixtures, sinks regularly used.
- the sample data shown in the graph in figure 10 refer to the hygienic conditions before (columns indicated with P) and after (columns indicated with D) the cleaning, with the enzyme mixture, respectively, of an area of the floor immediately below the toilet of a health facility.
- the data show an average 79% reduction of cfu.
- the data - shown in the graphs in figures 11, 12, 13, 14 - refer to the actual behavior of the preparation according to the present invention in a health-care facility, in the first 72 and 96 hours, respectively, after treatment with the preparation according to the invention (first application and ordinary condition).
- figure 11 is a representative graph of a test performed on a toilet seat of a healthcare facility which shows the time course of the total bacterial count (TBC) in the first 72 hours (3 days) after treatment with the preparation according to the invention.
- TBC total bacterial count
- figure 12 is a representative graph of a test performed on a sink of the bathroom of a hospital room of the same facility, showing the time course of the total bacterial count (TBC) in the first 72 hours after treatment.
- TBC total bacterial count
- Figure 13 is a representative graph of a test performed on a toilet seat of the facility, showing the time course of the total bacterial count (TBC) in the first 96 hours after treatment.
- figure 14 is a representative graph of a test performed on a sink of the bathroom of a hospital room of the facility, showing the time course of the total bacterial count (TBC) in the first 96 hours after treatment.
- TBC total bacterial count
- the associated check methods are microbiological checks, bioluminescence checks, colorimetric presence checks, operators accuracy checks with UV methodology.
- TBC total bacterial count
- This test focuses in particular on measurements taken 4-7 hours after the sanitization of the morning (at about 2:00-3:00 pm) to evaluate the efficacy of containment of the regrowth of the bacterial load.
- each reported value is the average result of two determinations carried out on the floor in the central areas of the rooms of the health-care or hospital facility in question.
- control samples originated with a standard cleaning procedure with sampling at 3:00 pm (according to the prior art), a 40 to 80% range of off-scale values (higher than the possibility of counting on the 24 cm 2 RODAC plates) was found.
- the preparation described above due to the features and efficacy also described in the embodiment examples illustrated, therefore allows, through the method according to the invention also described above, reducing the normal cleaning operations, as a result of the fact that dirt that has accumulated after the cleaning operation is continually degraded over time by the enzyme anchored to the surface and/or to the layers present on such a surface.
- the continuous degradation of the dirt from sources external to the surface and/or to the layers present on such a surface also causes the control and reduction of pathogens harmful to health that may proliferate due to the accumulation of fats, proteins, sugars that mainly constitute the composition thereof.
- the continuous degradation over time allows a reduction (in terms of time or number) of the routine (manual and mechanical) cleaning operations equal to 10%, instead by 30%, preferably by 50%.
- the materials used may be any, according to the requirements and to the prior art.
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EP0476915A2 (de) * | 1990-09-14 | 1992-03-25 | The Clorox Company | Lipase-Oberflächenkomplex und dessen Methoden zur Bildung und Gebrauch |
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EP0476915A2 (de) * | 1990-09-14 | 1992-03-25 | The Clorox Company | Lipase-Oberflächenkomplex und dessen Methoden zur Bildung und Gebrauch |
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CN103666780A (zh) * | 2012-09-22 | 2014-03-26 | 青岛道合生物科技有限公司 | 卫生间专用清洗除菌液 |
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