EP3009521A4 - Marker for detecting proliferation and treatment capacities of adipose-derived stem cell cultured in medium containing egf or bfgf, and use thereof - Google Patents

Marker for detecting proliferation and treatment capacities of adipose-derived stem cell cultured in medium containing egf or bfgf, and use thereof Download PDF

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Publication number
EP3009521A4
EP3009521A4 EP14810983.8A EP14810983A EP3009521A4 EP 3009521 A4 EP3009521 A4 EP 3009521A4 EP 14810983 A EP14810983 A EP 14810983A EP 3009521 A4 EP3009521 A4 EP 3009521A4
Authority
EP
European Patent Office
Prior art keywords
bfgf
adipose
marker
stem cell
medium containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP14810983.8A
Other languages
German (de)
French (fr)
Other versions
EP3009521A1 (en
EP3009521B1 (en
Inventor
Kyung Sook Chung
Mi Hyung Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Anterogen Co Ltd
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Anterogen Co Ltd
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Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB, Anterogen Co Ltd filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of EP3009521A1 publication Critical patent/EP3009521A1/en
Publication of EP3009521A4 publication Critical patent/EP3009521A4/en
Application granted granted Critical
Publication of EP3009521B1 publication Critical patent/EP3009521B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP14810983.8A 2013-06-12 2014-06-11 Marker for detecting proliferation and treatment capacities of adipose-derived stem cell cultured in medium containing egf or bfgf, and use thereof Active EP3009521B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020130067365A KR101603633B1 (en) 2013-06-12 2013-06-12 Detection markers of proliferation and therapeutic potency of adipocyte-derived stem cells cultured in media containing EGF or bFGF and use thereof
PCT/KR2014/005110 WO2014200256A1 (en) 2013-06-12 2014-06-11 Marker for detecting proliferation and treatment capacities of adipose-derived stem cell cultured in medium containing egf or bfgf, and use thereof

Publications (3)

Publication Number Publication Date
EP3009521A1 EP3009521A1 (en) 2016-04-20
EP3009521A4 true EP3009521A4 (en) 2017-01-04
EP3009521B1 EP3009521B1 (en) 2018-08-01

Family

ID=52022485

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14810983.8A Active EP3009521B1 (en) 2013-06-12 2014-06-11 Marker for detecting proliferation and treatment capacities of adipose-derived stem cell cultured in medium containing egf or bfgf, and use thereof

Country Status (6)

Country Link
US (1) US9874574B2 (en)
EP (1) EP3009521B1 (en)
JP (1) JP6793037B2 (en)
KR (1) KR101603633B1 (en)
CN (1) CN105264089B (en)
WO (1) WO2014200256A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101667001B1 (en) * 2013-08-12 2016-10-17 한국생명공학연구원 Secretory detection marker of proliferation and therapeutic potency of adipocyte-derived stem cells and use thereof
CN105368959B (en) * 2015-12-15 2019-01-04 甘肃中天羊业股份有限公司 Molecular labeling and its application of the ANXA10 gene as sheep immune character

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013162286A1 (en) * 2012-04-24 2013-10-31 한국생명공학연구원 Marker for detecting potency of adipose-derived stem cells, and use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090202659A1 (en) * 2005-06-10 2009-08-13 Gimble Jeffrey M Modulation of Peripheral Clocks in Adipose Tissue
WO2008061104A2 (en) * 2006-11-13 2008-05-22 Invitrogen Corporation Methods and kits for detecting prostate cancer biomarkers
WO2010037134A2 (en) * 2008-09-29 2010-04-01 Stemlifeline, Inc. Multi-stage stem cell carcinogenesis
EP2425844B1 (en) * 2009-04-28 2014-05-07 Anterogen Co., Ltd. Autologous and allogenic adipose-derived stromal stem cell composition for treating fistulas
WO2011072119A2 (en) * 2009-12-10 2011-06-16 The Research Foundation Of State University Of Newyork Stem-cell material and method of use
KR101639988B1 (en) * 2011-05-11 2016-07-15 (주)안트로젠 Method for preparation of mesenchymal stem cell culture comprising high concentration of cell growth factors and composition prepared therefrom

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013162286A1 (en) * 2012-04-24 2013-10-31 한국생명공학연구원 Marker for detecting potency of adipose-derived stem cells, and use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BYUNG-CHUL LEE ET AL: "PGE2 maintains self-renewal of human adult stem cells via EP2-mediated autocrine signaling and its production is regulated by cell-to-cell contact", SCIENTIFIC REPORTS, vol. 6, 27 May 2016 (2016-05-27), pages 26298, XP055321874, DOI: 10.1038/srep26298 *
GIMBLE JEFFREY M ET AL: "Adipose-derived stem cells for regenerative medicine.", CIRCULATION RESEARCH 11 MAY 2007, vol. 100, no. 9, 11 May 2007 (2007-05-11), pages 1249 - 1260, XP002764504, ISSN: 1524-4571 *
MANFERDINI CRISTINA ET AL: "Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2.", ARTHRITIS AND RHEUMATISM MAY 2013, vol. 65, no. 5, May 2013 (2013-05-01), pages 1271 - 1281, XP002764503, ISSN: 1529-0131 *
See also references of WO2014200256A1 *

Also Published As

Publication number Publication date
CN105264089B (en) 2018-11-16
EP3009521A1 (en) 2016-04-20
US20160178648A1 (en) 2016-06-23
KR101603633B1 (en) 2016-03-15
JP6793037B2 (en) 2020-12-02
EP3009521B1 (en) 2018-08-01
CN105264089A (en) 2016-01-20
US9874574B2 (en) 2018-01-23
JP2016521569A (en) 2016-07-25
KR20140145011A (en) 2014-12-22
WO2014200256A1 (en) 2014-12-18

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