EP3003043A2 - Boron added antimicrobial joint sealant - Google Patents

Boron added antimicrobial joint sealant

Info

Publication number
EP3003043A2
EP3003043A2 EP14739271.6A EP14739271A EP3003043A2 EP 3003043 A2 EP3003043 A2 EP 3003043A2 EP 14739271 A EP14739271 A EP 14739271A EP 3003043 A2 EP3003043 A2 EP 3003043A2
Authority
EP
European Patent Office
Prior art keywords
joint sealant
spp
borate
sealant material
material according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP14739271.6A
Other languages
German (de)
French (fr)
Inventor
Fikrettin Sahin
Selami DEMIRCI
Zeynep USTAOGLU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yeditepe Universitesi
Original Assignee
Yeditepe Universitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeditepe Universitesi filed Critical Yeditepe Universitesi
Publication of EP3003043A2 publication Critical patent/EP3003043A2/en
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14—Boron; Compounds thereof

Definitions

  • the present invention relates to an antimicrobial joint sealant obtained by adding boron derivatives therein.
  • Microorganisms such as molds, yeasts and bacteria can live on all known surfaces. These organisms, which are primary and opportunistic pathogens, lead to clinical diseases dangerous to humans.
  • Surface adsorption characteristic of the microorganisms is the main aspect that supports microbial life on surfaces. Microorganisms which adsorb to the surface proliferate by using the moisture in the air together with the inorganic and organic nutrients on the surfaces. Microorganisms, whose quantity has risen above a certain level, even if they are not primary pathogens, may be dangerous for individuals especially in hospital environments in which there are patients with suppressed or weak immune systems. Contaminated surfaces transfer their microbial loads to humans via inhalation, touching or nutrition. Especially contamination of the surfaces in hospitals increases hospital -acquired infection risks and affects mortality rate and hospitalization time (Sawrtz, 1994).
  • Bacteria and fungi can be shown as the most important reason for environmental diseases, epidemics, acute and chronic allergic risks not only in hospital environment but also in all of the other living spaces. It is observed that particularly molds and yeasts are isolated from all surfaces mainly toilet, bathroom and kitchen surfaces in previous studies. Hamada and Abe (2010) have reported in their study that they have isolated the fungal species such as Ramicloridium strelitzi e, Cyphellophora laciniata, Phoma fimeti, and Exophiala spp. from the bathroom surfaces even under different conditions. Proliferation of these microorganisms above a certain number increases the risk of infection and acute and chronic allergies. Many studies have shown that being exposed to fungus such as Alternaria spp.
  • Joint sealant powder made into a grout by mixing with an adequate amount of water, is used for filling in the spaces between the tiles applied on the wall or floor. Due to the cement it contains, the joint sealant grout dries and holds on to the area on which it is applied and ensures that no space remains between the tiles. Although the surface of the tile is water, oil and dust repellent, since the joint sealant between the tiles does not have this characteristic, all of the wastes accumulate on the surface of the joint sealant. These areas satiated with organic wastes and water become an extremely convenient area for microbial growth. Microorganisms settling on material surfaces, in addition to leading to infections, cause materials to wear out and deteriorate.
  • Microorganisms proliferate by using organic and inorganic components on materials in addition to the humidity in the air. During reproduction, they damage the structure of materials in different ways and make them dysfunctional. Thousands of health-threatening microorganisms varying according to temperature and humidity rate have been isolated from all of the surfaces and objects inside the house (Reponen et al., 2001). Microorganisms, which can proliferate at any place where they can access humidity and nutrients, cause abrasion on surfaces with the damaging end products they produce both due to using the organic and inorganic nutrients on the surface that they are growing and as a result of their metabolic activities. Thus, this type of surface abrasions may cause significant financial losses. It was determined that microbial abrasion is higher particularly at regions with high humidity rate.
  • boric acid has antibacterial activity on enteric bacteria.
  • Antimicrobial agents containing boron were applied on gram negative bacteria (Escherichia coli and Proteus mirabilis) and they were found to be effective.
  • boric esters have broad spectrum antibacterial activity. They have discovered in their study that boric esters inhibit DNA methyl transferase in gram negative and positive bacteria.
  • Reynold et al. (2007) showed that lipophilic 2,4-diamino-6-methylpyrimidine antifolate compound comprising two different borons has moderate antibacterial activity against the bacteria Mycobacterium avium and Lactobacillus case. In addition to these, it is shown that some boron derivatives have antifungal activities.
  • Qin et al. (2007) showed that potassium tetraborate has an inhibitory effect on micelle growth of Penicillium expansium.
  • the International patent document no. WO2006084161 discloses antimicrobial granules for use in various surface coating and grouting materials.
  • the boron added compositions in the state of the art are antibacterial whereas a study on antimicrobial (antibacterial + antifungal + anticandidal) joint sealant is not encountered.
  • An objective of the present invention is to provide an antimicrobial joint sealant containing boron derivatives.
  • Another objective of the present invention is to provide a joint sealant material which provides protection against microbial decomposition, corrosion and decay on the products and surfaces obtained by using antimicrobial joint sealant.
  • a further objective of the present invention is to provide a long lasting joint sealant which prevents the yellowing and discoloration resulting from microbial growth.
  • Table 1 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Zinc Borate (1/10 w/w).
  • Table 2 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Sodium Borate (1/10 w/w).
  • Table 3 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Potassium Borate (1/10 w/w).
  • the boron derivatives used in the experimental studies were sodium borate ((Na 2 O)(B 2 O 3 )5.10H 2 O), zinc borate (2Zn0.3B 2 0 3 .3.5H 2 0) and potassium borate ( ⁇ 2 ⁇ 4 ⁇ 7.4 ⁇ 2 0).
  • Modified disc diffusion test was employed in the antimicrobial activity tests.
  • At least one of sodium borate ((Na 2 O)(B 2 O 3 ) 5 .10H 2 O), zinc borate (2Zn0.3B 2 0 3 .3.5H 2 0) or potassium borate (K 2 B 4 0 7 .4H 2 0) boron derivatives in mineral form was mixed with a commercially available joint sealant at a ratio of 0,5- 20% (w/w). The homogenous mixtures were subjected to antimicrobial activity tests.
  • boric acid alkaline or alkaline earth metal borates
  • lithium borates such as lithium tetra borate, lithium metaborate, lithium pentaborate
  • sodium borates such as sodium metaborate, sodium hexaborate, sodium tetraborate, sodium octaborate
  • potassium borates such as potassium tetraborate, potassium metaborate, potassium hexaborate, potassium octaborate
  • calcium borates such as calcium diborate, calcium metaborate, calcium tetraborate, tricalcium tetraborate, pentacalcium tetraborate, calcium hexaborate
  • magnesium borates such as magnesium metaborate, magnesium diborate, trimagnesium tetraborate, pentamagnesium tetraborate) or all hydrate forms thereof, ammonium borates (ammonium metaborate,
  • Standard NCCLS disc diffusion method (Lalitha and Vellore, 2005) with modifications was used to determine the antimicrobial activity of zinc borate and sodium borate on each microorganism tested.
  • the lOOul solution containing 10 8 cfu/ml bacteria, 10 6 cfu/ml yeast and 10 4 spor/ml mold was prepared with new cultures and was inoculated on Nutrient Agar (NA), Sabouraud Dextrose Agar (SDA) and Potato Dextrose Agar (PDA), respectively. 20 ⁇ 1 of sterile water was dropped on the empty discs and it was separately immersed into the joint sealant compositions comprising pulverized zinc borate, sodium borate and potassium borates at a concentration of 0.5-10% (w/w).
  • the discs coded with the boron containing joint sealant materials were placed on inoculated petri dishes. Empty discs with 20ul drop of sterile water were used as negative control. Ofloxacin (10 ⁇ g/disc) and nystatin (30 ⁇ g/disc) were used as positive control groups for bacteria and fungi, respectively.
  • the joint sealant material containing sodium, potassium and zinc borate, applied at specific ratios, was determined to be effective against all tested microorganisms by the modified disc diffusion method, and the results are given in Table 1, 2, and 3.
  • a shows that the applied Sodium-Borate ratio is weight/weight
  • b + mark shows that joint sealant formulation produced inhibition zone.
  • - mark shows that joint sealant formulation did not produce inhibition zone.
  • a shows that the applied Potassium-Borate ratio is weight/weight
  • b + mark shows that joint sealant formulation produced inhibition zone.
  • - mark shows that joint sealant formulation did not produce inhibition zone.
  • Antimicrobial activity of the homogenous mixture (boron- joint sealant) containing/non-containing boron derivatives on microorganisms were determined by the modified disc diffusion test. It was found that the mixture prepared at a ratio of 1/10 (w/w) by all three boron derivatives exhibited antimicrobial effect. It was determined that the joint sealant mixture obtained with sodium, zinc and potassium borates were antibacterial (for bacteria), anticandidal (for yeasts) and antifungal (for fungi).
  • Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Botrytis spp., Aspergillus spp., Penicillium spp., Fusarium spp., Alternaria spp., Penicillium spp., and Candida spp. could be controlled by means of the boron derivatives added to the joint sealant materials.
  • the surfaces of all kinds of raw materials and semi finished products produced from the joint sealant material of the present invention provide protection against microbial contamination and degradation, corrosion and decay.
  • the present invention can be applied to cement, concrete, gypsum, liquid or powder paint, prefabrication materials gunite, glue, adhesive, polish or plaster instead of the joint sealant.
  • Antibacterial Agents Effects on Growth and Morphology of Bacteria Under Various Culture Conditions". Antimicrobial Agents and Chemotherapy, 17, 549, 1980.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Sealing Material Composition (AREA)

Abstract

The present invention relates to obtaining an antimicrobial joint sealant obtained by adding boron compounds therein. The surfaces of all kinds of raw materials and semi finished products produced from the joint sealant material of the present invention are protected against microbial contamination and degradation, corrosion and decay. In the invention wherein sodium borate ((Na2O)(B2O3)5.10H2O), zinc borate (2ZnO.3B2O3.3.5H2O) and potassium borate (K2B4O7.4H2O) are used as boron derivatives; it is determined that Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Botrytis spp., Aspergillus spp., Penicillium spp., Fusarium spp., Alternaria spp., Penicillium spp., and Candida spp. can be controlled by means of the said materials added to the joint sealant.

Description

DESCRIPTION
BORON ADDED ANTIMICROBIAL JOINT SEALANT
Field of the Invention
The present invention relates to an antimicrobial joint sealant obtained by adding boron derivatives therein.
Background of the Invention
Microorganisms such as molds, yeasts and bacteria can live on all known surfaces. These organisms, which are primary and opportunistic pathogens, lead to clinical diseases dangerous to humans. Surface adsorption characteristic of the microorganisms is the main aspect that supports microbial life on surfaces. Microorganisms which adsorb to the surface proliferate by using the moisture in the air together with the inorganic and organic nutrients on the surfaces. Microorganisms, whose quantity has risen above a certain level, even if they are not primary pathogens, may be dangerous for individuals especially in hospital environments in which there are patients with suppressed or weak immune systems. Contaminated surfaces transfer their microbial loads to humans via inhalation, touching or nutrition. Especially contamination of the surfaces in hospitals increases hospital -acquired infection risks and affects mortality rate and hospitalization time (Sawrtz, 1994).
Bacteria and fungi can be shown as the most important reason for environmental diseases, epidemics, acute and chronic allergic risks not only in hospital environment but also in all of the other living spaces. It is observed that particularly molds and yeasts are isolated from all surfaces mainly toilet, bathroom and kitchen surfaces in previous studies. Hamada and Abe (2010) have reported in their study that they have isolated the fungal species such as Ramicloridium strelitzi e, Cyphellophora laciniata, Phoma fimeti, and Exophiala spp. from the bathroom surfaces even under different conditions. Proliferation of these microorganisms above a certain number increases the risk of infection and acute and chronic allergies. Many studies have shown that being exposed to fungus such as Alternaria spp. too much causes allergic rhinitis and asthma in children (Arshad et al., 2005; Downs et al., 2001>; Halonen et al., 1997) Microorganisms, particularly fungi, are often associated with allergic asthma, rhinitis, bronchopulmonary mycosis and hypersensitivity pneumonitis. It is estimated that the incidence of respiratory allergies caused by molds is 20-30% in atopic people and 6% in the general population (Kurup et al. 2000). Thus, rendering the filling materials (e.g. joint sealant), which provides a convenient medium for microbial growth, antimicrobial particularly at areas such as toilets, bathrooms and kitchens where humidity rate is high, will enable to reduce microbial acute, chronic allergies and infections. Joint sealant powder, made into a grout by mixing with an adequate amount of water, is used for filling in the spaces between the tiles applied on the wall or floor. Due to the cement it contains, the joint sealant grout dries and holds on to the area on which it is applied and ensures that no space remains between the tiles. Although the surface of the tile is water, oil and dust repellent, since the joint sealant between the tiles does not have this characteristic, all of the wastes accumulate on the surface of the joint sealant. These areas satiated with organic wastes and water become an extremely convenient area for microbial growth. Microorganisms settling on material surfaces, in addition to leading to infections, cause materials to wear out and deteriorate. Microorganisms proliferate by using organic and inorganic components on materials in addition to the humidity in the air. During reproduction, they damage the structure of materials in different ways and make them dysfunctional. Thousands of health-threatening microorganisms varying according to temperature and humidity rate have been isolated from all of the surfaces and objects inside the house (Reponen et al., 2001). Microorganisms, which can proliferate at any place where they can access humidity and nutrients, cause abrasion on surfaces with the damaging end products they produce both due to using the organic and inorganic nutrients on the surface that they are growing and as a result of their metabolic activities. Thus, this type of surface abrasions may cause significant financial losses. It was determined that microbial abrasion is higher particularly at regions with high humidity rate.
Vincke asserted that 10% of the total waste water consumption in Belgium is caused by the abrasion dependent on microbial growth (2000). In addition to surface abrasion, microbial growth and the end products dependent thereon cause oxidation on the surfaces leading to yellowing and discoloration of the joint sealant. Since this undesirable situation cannot be eliminated even if the surface is cleaned, a new joint sealant is required to be applied. Since the antimicrobial joint sealant that will be developed will not allow microbial growth, it will be functional for a long period of time without losing its color and characteristics. In the literature, it has been previously shown that some boron derivatives had an inhibitory effect on microbial growth. Bailey et al. (1980) proved, with the experiments they conducted, that boric acid has antibacterial activity on enteric bacteria. Antimicrobial agents containing boron were applied on gram negative bacteria (Escherichia coli and Proteus mirabilis) and they were found to be effective.
In another study, Benkovic et al. (2005) showed that boric esters have broad spectrum antibacterial activity. They have discovered in their study that boric esters inhibit DNA methyl transferase in gram negative and positive bacteria. Reynold et al. (2007) showed that lipophilic 2,4-diamino-6-methylpyrimidine antifolate compound comprising two different borons has moderate antibacterial activity against the bacteria Mycobacterium avium and Lactobacillus case. In addition to these, it is shown that some boron derivatives have antifungal activities. Qin et al. (2007) showed that potassium tetraborate has an inhibitory effect on micelle growth of Penicillium expansium. It was determined that 0.1% concentration of potassium tetraborate is the minimum inhibitory concentration that prevents micelle growth. Qin et al. (2010) also investigated the effects of potassium tetraborate on Botrytis cinerea which is the pathogen of gray mold disease. They showed that they could control this mold causing disease on the grapes by using potassium tetraborate (1 %).
The International patent document no. WO2006084161 discloses antimicrobial granules for use in various surface coating and grouting materials.
The boron added compositions in the state of the art are antibacterial whereas a study on antimicrobial (antibacterial + antifungal + anticandidal) joint sealant is not encountered.
Summary of the Invention An objective of the present invention is to provide an antimicrobial joint sealant containing boron derivatives.
Another objective of the present invention is to provide a joint sealant material which provides protection against microbial decomposition, corrosion and decay on the products and surfaces obtained by using antimicrobial joint sealant.
A further objective of the present invention is to provide a long lasting joint sealant which prevents the yellowing and discoloration resulting from microbial growth. Detailed Description of the Invention
The tables related to the boron added antimicrobial joint sealant developed to fulfill the objectives of the present invention are given below, wherein;
Table 1 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Zinc Borate (1/10 w/w).
Table 2 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Sodium Borate (1/10 w/w).
Table 3 shows the results of the modified disc diffusion test conducted with joint sealant materials containing/not containing Potassium Borate (1/10 w/w).
Experimental studies
The boron derivatives used in the experimental studies were sodium borate ((Na2O)(B2O3)5.10H2O), zinc borate (2Zn0.3B203.3.5H20) and potassium borate (Κ2Β4θ7.4Η20). Modified disc diffusion test was employed in the antimicrobial activity tests.
At least one of sodium borate ((Na2O)(B2O3)5.10H2O), zinc borate (2Zn0.3B203.3.5H20) or potassium borate (K2B407.4H20) boron derivatives in mineral form was mixed with a commercially available joint sealant at a ratio of 0,5- 20% (w/w). The homogenous mixtures were subjected to antimicrobial activity tests.
In addition to sodium borate, zinc borate and potassium borate which are the basic boron derivatives used in the embodiment of the invention; boric acid, alkaline or alkaline earth metal borates (lithium borates such as lithium tetra borate, lithium metaborate, lithium pentaborate; sodium borates such as sodium metaborate, sodium hexaborate, sodium tetraborate, sodium octaborate; potassium borates such as potassium tetraborate, potassium metaborate, potassium hexaborate, potassium octaborate; calcium borates such as calcium diborate, calcium metaborate, calcium tetraborate, tricalcium tetraborate, pentacalcium tetraborate, calcium hexaborate; magnesium borates such as magnesium metaborate, magnesium diborate, trimagnesium tetraborate, pentamagnesium tetraborate) or all hydrate forms thereof, ammonium borates (ammonium metaborate, ammonium tetraborate, ammonium pentaborate and ammonium octaborate), boric acid esters (monomethyl borate, dimethyl borate, trimethyl borate, monoethyl borate, diethyl borate, triethyl borate, monopropyl borate, dipropyl borate, tripropyl borate, monobutyl borate, dibutyl borate or tributyl borate) can be used.
Modified disc diffusion method
Standard NCCLS disc diffusion method (Lalitha and Vellore, 2005) with modifications was used to determine the antimicrobial activity of zinc borate and sodium borate on each microorganism tested. The lOOul solution containing 108 cfu/ml bacteria, 106 cfu/ml yeast and 104 spor/ml mold was prepared with new cultures and was inoculated on Nutrient Agar (NA), Sabouraud Dextrose Agar (SDA) and Potato Dextrose Agar (PDA), respectively. 20μ1 of sterile water was dropped on the empty discs and it was separately immersed into the joint sealant compositions comprising pulverized zinc borate, sodium borate and potassium borates at a concentration of 0.5-10% (w/w). The discs coded with the boron containing joint sealant materials were placed on inoculated petri dishes. Empty discs with 20ul drop of sterile water were used as negative control. Ofloxacin (10μg/disc) and nystatin (30μg/disc) were used as positive control groups for bacteria and fungi, respectively. The petri dishes, which were inoculated and on which modified disc diffusion method was applied, were kept at 36+1 °C for bacteria for 24 hours and for yeasts for 48 hours and at 25+1 °C for fungi for 72 hours. Antimicrobial activity was evaluated by measuring the zone of inhibition (area where microorganisms do not grow) against the test microorganisms. All tests were repeated at least twice. Experiment Results
The joint sealant material containing sodium, potassium and zinc borate, applied at specific ratios, was determined to be effective against all tested microorganisms by the modified disc diffusion method, and the results are given in Table 1, 2, and 3.
Table 1. Results of the modified disc diffusion test conducted with joint sealant material containing/not containing Zinc Borate (1/10 w/wa)
a shows that the applied Zinc-Borate ratio is weight/weight,
b + mark shows that joint sealant formulation produced inhibition zone. - mark shows that joint sealant formulation did not produce inhibition zone. Table 2. Results of the modified disc diffusion test conducted with joint sealant material containing/not containing Sodium Borate (1/10 w/wa)
a shows that the applied Sodium-Borate ratio is weight/weight, b + mark shows that joint sealant formulation produced inhibition zone. - mark shows that joint sealant formulation did not produce inhibition zone.
Table 3. Results of the modified disc diffusion test conducted with joint sealant material containing/not containing Potassium Borate (1/10 w/wa)
a shows that the applied Potassium-Borate ratio is weight/weight, b + mark shows that joint sealant formulation produced inhibition zone. - mark shows that joint sealant formulation did not produce inhibition zone.
Antimicrobial activity of the homogenous mixture (boron- joint sealant) containing/non-containing boron derivatives on microorganisms were determined by the modified disc diffusion test. It was found that the mixture prepared at a ratio of 1/10 (w/w) by all three boron derivatives exhibited antimicrobial effect. It was determined that the joint sealant mixture obtained with sodium, zinc and potassium borates were antibacterial (for bacteria), anticandidal (for yeasts) and antifungal (for fungi).
It was observed that Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Botrytis spp., Aspergillus spp., Penicillium spp., Fusarium spp., Alternaria spp., Penicillium spp., and Candida spp. could be controlled by means of the boron derivatives added to the joint sealant materials.
The surfaces of all kinds of raw materials and semi finished products produced from the joint sealant material of the present invention provide protection against microbial contamination and degradation, corrosion and decay.
The present invention can be applied to cement, concrete, gypsum, liquid or powder paint, prefabrication materials gunite, glue, adhesive, polish or plaster instead of the joint sealant.
References
Antoci V. Jr., S.B. King, B. Jose, J. Parvizi, A.R. Zeiger and E. Wickstrom, "Vancomycin covalently bonded to titanium alloy prevents bacterial colonization", Journal of Orthopaedic Research, 25, 858-866, 2007.
Arshad S.H., S.M. Tariq, S. Matthews, and E. Hakim, "Sensitization to common allergens and its association with allergic disorders at age 4 years: a whole population birth cohort study". Pediatrics, 108, 33-37, 2005.
Bailey P. J., G. Cousins, G. A. Snow, and A.J. White, "1. Boron-Containing
Antibacterial Agents: Effects on Growth and Morphology of Bacteria Under Various Culture Conditions". Antimicrobial Agents and Chemotherapy, 17, 549, 1980.
Benkovic S.J., S.J. Baker, and Alley M.R., "Identification of borinic esters as inhibitors of bacterial cell growth and bacterial methyltransferases, CcrM and MenH". Journal of Medicinal Chemistry. 48, 7468-7476, 2005.
Cook, G., J. W. Costerton, and R. O. Darouiche, "Direct confocal microscopy studies of the bacterial colonization in vitro of a silver-coated heart valve sewing cuff, International Journal of Antimicrobial Agents, 13, 169-173, 2000.
Downs S.H., Mitakakis T.Z., Marks G.B., Car N.G., Belousova E.G., Leuppi J.D., Xuan W., Downie S.R., Tobias A., and Peat J.K., "Clinical importance of Alternaria expo-sure in children" American Journal of Respiratory and Critical Care Medicine 164, 455^159, 2001.
Halonen M, Stern D.A., Wright A.L., Taussig L.M., and Martinez F.D., "Alternaria as a major allergen for asthma in children raised in a desert environment", American Journal of Respiratory and Critical Care Medicine 155, 1356-1361, 1997.
Hamada N. and N. Abe, "Growth Characteristics of Four Fungal Species in Bathrooms", Biocontrol Science, 15, 111 -115, 2010.
13 Huang J., H. Murata, R. R. Koepsel, A. J. Russell and K. Matyjaszewsk, "Antibacterial Polypropylene via Surface-Initiated Atom Transfer Radical Polymerization", Biomacromolecules, 8, 1396-1399, 2007.
Kurup V.P., Shen H.D., and Banerjee B, "Respiratory fungal allergy", Microbes and Infection, 2, 1101-1 110, 2000.
Lalitha, M. K. and T. N. Vellore, "Manual on antimicrobial susceptibility testing", URL: http://www. ijmm. org/documents/Antimicrobial.doc, 2005.
Lee S. B., R. R. Koepsel, S. W. Morley, K. Matyjaszewski, Y. Sun and A. J. Russel, "Permanent, Nonleaching Antibacterial Surfaces. 1. Synthesis by Atom Transfer Radical Polymerization", Biomacromolecules, 5, 877-882, 2004.
Neely A.N. and M.P. Maley, "Survival of Enterococci and Staphylococci on Hospital Fabrics and Plastic", Journal of Clinical Microbiology, 38, 724-726, 2000.
Qin G., S. Tian, Z. Chan, and B. Li, "Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum" Molecular & Cellular Proteomics, 6, 425-Ψ38, 2007.
Qin G.,Y. Zong, Q. Chen, D. Hua, and S. Tian, "Inhibitory effect of boron against Botrytis cinerea on table grapes and its possible mechanisms of action", International Journal of Food Microbiology 138, 145-150, 2010.
Reponen T., Willeke K., Grishpun S., and Nevalainen A., "Biological particle sampling", In: Baron, P. A. and Willeke, K. (eds), Aerosol Measurement: Principles, Techniques and Application, 2nd ed., New York, Wiley Interscience, 2001
Reynolds, R.C., Campbell S.R., Fairchild R.G., Kisliuk R.L., Micca P.L., Queener S.F., Riordan J.M., SedwickW.D., Waud W.R., Leung A.K.W., Dixon R.W., Suling W.J., BorhaniD.W., "Novel boron-containing, nonclassical antifolates: Synthesis and preliminary biological and structural evaluation. Journal of Medicinal Chemistry, 50, 3283-3289, 2007.
14 Sawrtz, M. N., "Hospital-acquired infections: Diseases with increasingly limited therapies", Proceedings of the National Academy of Sciences of the United States of America, 91, 2420-2427, 1994.
Tazaki K., T. Mori, and T. Nonaka, "Microbial Jarosite and Gypsum from Corrosion of Portland Cement Concrete", Canadian Mineralogist, 30, 43-444, 1992.
Vincke E., "Biogenic sulfuric acid corrosion of concrete: microbial interaction, simulation and prevention", PhD thesis, Ghent, GhentUniversity, 2002.
15

Claims

1. An antimicrobial joint sealant material containing boron derivatives.
The joint sealant material according to Claim 1, containing sodium borate ((Na2O)(B2O3)5.10H2O).
3. The joint sealant material according to Claim 1, containing zinc borate (2Zn0.3B203.3.5H20).
4. The joint sealant material according to Claim 1, containing potassium borate (K2B407.4H20).
5. The joint sealant material according to Claims 1 to 4, obtained by mixing at least one of sodium borate ((Na2O)(B2O3)5.10H2O), zinc borate
(2Zn0.3B203.3.5H20) or potassium borate (K2B407.4H20) with a joint sealant at a ratio of 0.5-20% (w/w).
6. The joint sealant material according to Claims 1 to 6, which is antibacterial, anticandidal and antifungal.
7. The joint sealant material according to Claims 1 to 7, which can control Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Botrytis spp., Aspergillus spp., Penicillium spp., Fusarium spp., Alternaria spp., Penicillium spp., and Candida spp..
8. The joint sealant material according to Claims 1 to 7, which can be applied to cement, concrete, gypsum, all kinds of paints, prefabrication materials, gunite, glue, adhesive, polish and plaster instead of the joint sealant.
11
9. The joint sealant material according to Claim 1 , which comprises boric acid, alkaline or alkaline earth metal borates or all hydrates forms thereof, ammonium borates, boric acid esters.
12
EP14739271.6A 2013-06-03 2014-06-03 Boron added antimicrobial joint sealant Ceased EP3003043A2 (en)

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TR201306616 2013-06-03
PCT/TR2014/000145 WO2014196940A2 (en) 2013-06-03 2014-06-03 Boron added antimicrobial joint sealant

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EP3003043A2 true EP3003043A2 (en) 2016-04-13

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