EP2931739A1 - Sequence consensus kozak de nannochloropsis - Google Patents

Sequence consensus kozak de nannochloropsis

Info

Publication number
EP2931739A1
EP2931739A1 EP12889647.9A EP12889647A EP2931739A1 EP 2931739 A1 EP2931739 A1 EP 2931739A1 EP 12889647 A EP12889647 A EP 12889647A EP 2931739 A1 EP2931739 A1 EP 2931739A1
Authority
EP
European Patent Office
Prior art keywords
seq
cell
sequence
eukaryotic
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12889647.9A
Other languages
German (de)
English (en)
Other versions
EP2931739A4 (fr
Inventor
Peter DE HOFF
Leah Soriaga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ExxonMobil Technology and Engineering Co
Original Assignee
ExxonMobil Research and Engineering Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ExxonMobil Research and Engineering Co filed Critical ExxonMobil Research and Engineering Co
Publication of EP2931739A1 publication Critical patent/EP2931739A1/fr
Publication of EP2931739A4 publication Critical patent/EP2931739A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des séquences consensus Kozak de Nannochloropsis utilisables dans le cadre de l'expression de protéines dans des cellules eucaryotes, telles que des cellules algales. L'invention concerne, en outre, des constructions d'expression, des cassettes d'expression, des vecteurs de clonage ou d'expression et des cellules hôtes eucaryotes, telles que des cellules algales, ainsi que des procédés d'expression de protéines d'intérêt qui utilisent les séquences consensus Kozak décrites ici.
EP12889647.9A 2012-12-05 2012-12-05 Sequence consensus kozak de nannochloropsis Withdrawn EP2931739A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2012/067851 WO2014088563A1 (fr) 2012-12-05 2012-12-05 Séquence consensus kozak de nannochloropsis
US13/705,284 US9309523B2 (en) 2012-12-05 2012-12-05 Nannochloropsis kozak consensus sequence

Publications (2)

Publication Number Publication Date
EP2931739A1 true EP2931739A1 (fr) 2015-10-21
EP2931739A4 EP2931739A4 (fr) 2016-08-03

Family

ID=50825803

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12889647.9A Withdrawn EP2931739A4 (fr) 2012-12-05 2012-12-05 Sequence consensus kozak de nannochloropsis

Country Status (3)

Country Link
US (1) US9309523B2 (fr)
EP (1) EP2931739A4 (fr)
WO (1) WO2014088563A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105255912B (zh) * 2014-07-16 2019-07-12 中国科学院青岛生物能源与过程研究所 一种具有三脂酰甘油合成功能的基因及其应用
WO2023081792A2 (fr) * 2021-11-04 2023-05-11 Colorado State University Research Foundation Compositions d'algues eucaryotes et procédés associés

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5661017A (en) 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
WO2000062601A1 (fr) 1999-04-15 2000-10-26 Northeastern University Transformations genetiques d'algues marines multicellulaires fondees sur agrobacterium, souches resultantes et leurs produits
WO2003072775A1 (fr) 2002-02-28 2003-09-04 Takara Bio Inc. Promoteur, intron et terminateur d'origine algale
WO2003100045A1 (fr) * 2002-05-23 2003-12-04 Wolfgang Knecht Thymidines kinases d'origine vegetale et leurs applications
EP2172536B1 (fr) * 2003-08-01 2016-05-04 BASF Plant Science GmbH Procédé de production d'acides gras polyinsaturés chez des organismes transgéniques
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
WO2007133558A2 (fr) 2006-05-09 2007-11-22 The Scripps Research Institute Expression vigoureuse d'une protéine mammalienne bioactive dans des chloroplastes de chlamydomonas
US8552168B2 (en) 2007-05-11 2013-10-08 The Regents Of The University Of California Nucleic acids for inducing expression of transcripts and proteins and methods for making and using them
EP2297326A4 (fr) 2008-06-06 2011-11-16 Aurora Biofuels Inc Vecteurs à base de vcp pour la transformation de cellules d'algues
US8314228B2 (en) 2009-02-13 2012-11-20 Aurora Algae, Inc. Bidirectional promoters in Nannochloropsis
US20110014708A1 (en) 2009-07-17 2011-01-20 National Taiwan University Nucleic acid for use in algae and use thereof
EP2477478B1 (fr) 2009-09-15 2016-11-30 Sapphire Energy, Inc. Système de transformation du génome chloroplastique des espèces scenedesmus et dunaliella
KR20110062979A (ko) * 2009-12-04 2011-06-10 주식회사 에코파이코텍 Ｎａｎｎｏｃｈｌｏｒｏｐｓｉｓ ＥＰ1 미세조류 배양용 배지 조성물 및 상기 미세조류의 용도

Also Published As

Publication number Publication date
US9309523B2 (en) 2016-04-12
WO2014088563A1 (fr) 2014-06-12
EP2931739A4 (fr) 2016-08-03
US20140154740A1 (en) 2014-06-05

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