EP2864494B1 - Bandelette analytique électrochimique à chambres réceptrices d'échantillons en intersection - Google Patents

Bandelette analytique électrochimique à chambres réceptrices d'échantillons en intersection Download PDF

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Publication number
EP2864494B1
EP2864494B1 EP13733247.4A EP13733247A EP2864494B1 EP 2864494 B1 EP2864494 B1 EP 2864494B1 EP 13733247 A EP13733247 A EP 13733247A EP 2864494 B1 EP2864494 B1 EP 2864494B1
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Prior art keywords
sample
receiving chamber
electrochemical
test strip
analytical test
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German (de)
English (en)
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EP2864494A1 (fr
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Lynsey Whyte
Scott Sloss
Neil Whitehead
David Mccoll
Antony Smith
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LifeScan Scotland Ltd
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LifeScan Scotland Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

Definitions

  • the present invention relates, in general, to medical devices and, in particular, to analytical test strips and related methods.
  • the determination (e.g., detection and/or concentration measurement) of an analyte in a fluid sample is of particular interest in the medical field. For example, it can be desirable to determine glucose, ketone bodies, cholesterol, lipoproteins, triglycerides, acetaminophen and/or HbA1c concentrations in a sample of a bodily fluid such as urine, blood, plasma or interstitial fluid. Such determinations can be achieved using analytical test strips, based on, for example, visual, photometric or electrochemical techniques. Conventional electrochemical-based analytical test strips are described in, for example, U.S. Patent Nos. 5,708,247 , US 2011/0155589 and 6,284,125 .
  • intersection refers to entities (such as a first sample-receiving chamber and a second sample-receiving chamber) forming an intersection with each other; crossing each other, or overlapping each other.
  • intersect refers to a point or set of points common to two or more geometric entities (such as two sample-receiving chambers).
  • an electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) and/or a characteristic of the bodily fluid sample (for example, hematocrit) includes a first sample-receiving chamber with first and second sample-application openings, and first and second electrodes.
  • the first and second electrodes are disposed in the first sample-receiving chamber between the first and second sample-application openings.
  • the electrochemical-based analytical test strip also includes a second sample-receiving chamber and a plurality of electrodes disposed in the second sample-receiving chamber.
  • the second sample-receiving chamber intersects the first sample-receiving chamber between the first and second electrodes, thereby defining a chamber intersection.
  • Electrochemical-based analytical test strips are beneficial in that, for example, a relatively small volume of bodily fluid sample (for example, a bodily fluid sample of approximately 1.3 micro-liters) can be employed to fill both the first and second sample-receiving chambers.
  • a relatively small bodily fluid sample is enabled since the chamber intersection serves as the bodily fluid sample entrance to the second sample-receiving chamber and either of the first and second sample-application openings can be employed to apply a bodily fluid sample that fills both the first and the second sample-receiving chambers.
  • first and second electrodes disposed in the first sample-receiving chamber are on either side of the chamber intersection, a beneficially large separation of the first and second electrodes is achieved while maintaining a low bodily fluid sample volume.
  • electrochemical-based analytical test strips according to embodiments of the present invention can be manufactured using relatively inexpensive and simple conventional processes and materials.
  • FIG. 1 is a simplified exploded view of an electrochemical-based analytical test strip 100 according to an embodiment of the present invention.
  • FIG. 2 is a sequence of simplified top views of various layers of electrochemical-based analytical test strip 100.
  • FIG. 3 is a simplified top view of a portion of a patterned conductor layer of electrochemical-based analytical test strip 100.
  • FIG. 4 is a simplified top view of a portion of the patterned conductor layer and an enzymatic reagent layer of electrochemical-based analytical test strip 100, with the enzymatic reagent layer depicted as partially transparent to highlight the patterned conductor layer thereunder.
  • FIG. 5 is a simplified top view of the portion of the patterned conductor layer, the enzymatic reagent layer and a portion of a patterned spacer layer of electrochemical-based analytical test strip100.
  • electrochemical-based analytical test strip 100 for the determination of an analyte (such as glucose) in a bodily fluid sample includes an electrically-insulating substrate layer 110, a patterned conductor layer 120, an enzymatic reagent layer 130, a patterned spacer layer 140, a hydrophilic layer 150, and a top layer 160.
  • first sample-receiving chamber 162 includes a first sample-application opening 166 and a second sample-application opening 168.
  • first electrode 120a is disposed in the first sample-receiving chamber 162 between the first sample-application opening 166 and the second sample-application opening 168 and second electrode 120b disposed in first sample-receiving chamber 162 between the first sample-application opening 166 and the second sample-application opening 168.
  • second sample-receiving chamber 164 intersects the first sample-receiving chamber 162 between the first electrode 120a and the second electrode 120b, thereby defining a chamber intersection 170 in electrochemical-based analytical test strip 100.
  • electrodes 120c, 120d, and 120e are operatively disposed in second sample-receiving chamber 164.
  • electrochemical-based analytical test strip 100 is depicted as including a total of five electrodes
  • embodiments of electrochemical-based analytical test strips, including embodiments of the present invention can include any suitable number of electrodes.
  • First and second electrodes 120a and 120b, respectively can have areas of, for example, 0.23 square-mm.
  • Working electrodes 120c and 120d can each have, for example, an area of 0.28 square-mm and counter/reference electrode 120e can have, for example, an area of 0.56 square-mm.
  • the distance between the midpoints of the first and second electrodes i.e., in the left-to-right direction of FIG. 5 ) is, for example, 4.60mm.
  • Patterned conductor layer 120 including electrodes 120a, 120b, 120c, 120d and 120e, of analytical test strip 100 can be formed of any suitable material including, for example, gold, palladium, platinum, indium, titanium-palladium alloys and electrically conducting carbon-based materials including carbon inks.
  • first working electrode 120c, second working electrode 120d and counter/reference electrode 120e and enzymatic reagent layer 130 are such that analytical test strip 100 is configured for the electrochemical determination of an analyte (glucose) in a bodily fluid sample (whole blood) that has filled second sample-receiving chamber 164.
  • first electrode 120a and second electrode 120b are disposed in first sample-receiving chamber 162 such that electrochemical-based analytical test strip 100 is configured for the determination of haematocrit in a whole blood sample that has filled first sample-receiving chamber 162.
  • a bodily fluid sample is applied to electrochemical-based analytical test strip 100 and transferred to both first sample-receiving chamber 162 (thereby operatively contacting the first and second electrodes 120a and 120b) and to the second sample-receiving chamber 164, thereby operatively contacting electrodes 120c, 120d and 120e.
  • the determination of haematocrit using electrodes of an analytical test strip is described in, for example, U.S. Patent Application Nos. 61/581,100 ; 61/581,097 ; 61/581,089 ; 61/530,795 and 61/530,808 .
  • Chamber intersection 170 is configured to serve both as a portion of first sample-receiving chamber 162 and as a sample entrance for second sample-receiving chamber 164. Such a configuration beneficially minimizes the volume of the first and second sample-receiving chamber. Moreover, since first sample-receiving chamber 162 is reagent-less (i.e., enzymatic reagent layer 130 is not disposed within first sample-receiving chamber 162), there is no risk of a bodily fluid sample that flows through first sample-receiving chamber inadvertently introducing an unwanted reagent into the second sample-receiving chamber.
  • first sample-receiving chamber 162 and second sample-receiving chamber 164 are disposed in an essentially T-shaped configuration.
  • T-shaped configuration enables first and second sample-application openings 166 and 168 to be disposed on opposing lateral edges of electrochemical-based analytical test strip 100.
  • a user is thus enabled to choose either of the sample-application openings for application of a bodily fluid sample.
  • first sample-receiving chamber 162 has a width (in the vertical direction of the perspective of FIG. 5 ) of, for example, 0.75 microns while second sample-receiving chamber 164 has a width (in the horizontal direction of the perspective of FIG. 5 ) in the vicinity of the electrodes of, for example, 1.3 microns.
  • Electrically-insulating substrate layer 110 can be any suitable electrically-insulating substrate layer known to one skilled in the art including, for example, a nylon substrate, polycarbonate substrate, a polyimide substrate, a polyvinyl chloride substrate, a polyethylene substrate, a polypropylene substrate, a glycolated polyester (PETG) substrate, or a polyester substrate.
  • the electrically-insulating substrate layer can have any suitable dimensions including, for example, a width dimension of about 5 mm, a length dimension of about 27 mm and a thickness dimension of about 0.5 mm.
  • Electrically-insulating substrate layer 110 provides structure to electrochemical-based analytical test strip 100 for ease of handling and also serves as a base for the application (e.g., printing or deposition) of subsequent layers (e.g., a patterned conductor layer).
  • patterned conductor layers employed in analytical test strips according to embodiments of the present invention can take any suitable shape and be formed of any suitable materials including, for example, metal materials and conductive carbon materials.
  • Patterned spacer layer 140 can be formed, for example, from a screen-printable pressure sensitive adhesive commercially available from Apollo Adhesives, Tamworth, Staffordshire, UK. In the embodiment of FIGs. 1 through 5 , patterned spacer layer 140 defines outer walls of the first sample-receiving chamber 162 and the second sample-receiving chamber 164. Patterned spacer layer 140 can have a thickness of, for example, approximately 130 microns.
  • Hydrophilic layer 150 can be, for example, a clear film with hydrophilic properties that promote wetting and filling of electrochemical-based analytical test strip 100 by a fluid sample (e.g., a whole blood sample). Such clear films are commercially available from, for example, 3M of Minneapolis, Minnesota U.S.A. and Coveme (San Lazzaro di Savena, Italy). Hydrophilic layer 150 can be, for example, a polyester film coated with a surfactant that provides a hydrophilic contact angle ⁇ 10 degrees. Hydrophilic layer 150 can also be a polypropylene film coated with a surfactant or other surface treatment, e.g., a MESA coating. Hydrophilic layer 150 can have a thickness, for example, of approximately 100um.
  • hydrophilic layer 150 is patterned to provide an air vent for second sample-receiving chamber 164 (as depicted by the alignment of the patterned hydrophilic layer 150 and patterned spacer layer 140 in FIGs. 1 and 2 ).
  • Enzymatic reagent layer 130 can include any suitable enzymatic reagents, with the selection of enzymatic reagents being dependent on the analyte to be determined. For example, if glucose is to be determined in a blood sample, enzymatic reagent layer 130 can include a glucose oxidase or glucose dehydrogenase along with other components necessary for functional operation. Enzymatic reagent layer 130 can include, for example, glucose oxidase, tri-sodium citrate, citric acid, polyvinyl alcohol, hydroxyl ethyl cellulose, potassium ferrocyanide, antifoam, cabosil, PVPVA, and water. Further details regarding enzymatic reagent layers, and electrochemical-based analytical test strips in general, are in U.S. Patent Nos. 6,241,862 and 6,733,655 , the contents of which are hereby fully incorporated by reference.
  • Electrochemical-based analytical test strip 100 can be manufactured, for example, by the sequential aligned formation of patterned conductor layer 120, enzymatic reagent layer 130, patterned spacer layer 140, hydrophilic layer 150 and top layer 160 onto electrically-insulating substrate layer 110. Any suitable techniques known to one skilled in the art can be used to accomplish such sequential aligned formation, including, for example, screen printing, photolithography, photogravure, chemical vapour deposition and tape lamination techniques.
  • FIG. 6 is a flow diagram depicting stages in a method 600 for determining an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) and/or a characteristic of the bodily fluid sample (e.g., hematocrit) according to an embodiment of the present invention.
  • Method 600 includes (see step 610 of FIG. 6 ) applying a bodily fluid sample to either of a first sample-application opening and a second sample-application opening of a first sample-receiving chamber of an electrochemical-based analytical test strip such that the applied bodily fluid sample fills the first sample-receiving chamber and a second-sample-receiving chamber of the electrochemical-based analytical test strip.
  • Method 600 also includes measuring a first response of the electrochemical-based analytical test strip (for example an electrochemical response from electrodes in the second sample-receiving chamber) and determining an analyte in the bodily fluid sample is determined based on the measured first response (see steps 620 and 630 of FIG. 6 ).
  • a first response of the electrochemical-based analytical test strip for example an electrochemical response from electrodes in the second sample-receiving chamber
  • determining an analyte in the bodily fluid sample is determined based on the measured first response (see steps 620 and 630 of FIG. 6 ).
  • steps 640 and 650 of method 600 also includes, measuring a second response of the electrochemical-based analytical test strip (for example, an electrical response from electrodes in the first sample-receiving chamber) and determining a characteristic of the bodily fluid sample (such as hematocrit or other bodily fluid sample characteristic that can be determined in a reagent-less manner) based on the second measured response.
  • steps 640 and 650 can be employed to measure a second analyte (such as, for example, uric acid, acetaminophen or dopamine) of the bodily fluid sample in a reagent-less manner.
  • the measuring and determination steps described above can, if desired, by performed using a suitable associated meter and measurement steps 620 and 630 can be performed in any suitable sequence or in an overlapping manner.
  • method 600 can be readily modified to incorporate any of the techniques, benefits and characteristics of electrochemical-based analytical test strips according to embodiments of the present invention and described herein.

Claims (17)

  1. Bandelette réactive d'analyse électrochimique pour la détermination d'un analyte dans un échantillon de fluide corporel, la bandelette réactive d'analyse électrochimique comprenant :
    une première chambre de réception d'échantillon avec :
    une première ouverture d'application d'échantillon ; et
    une deuxième ouverture d'application d'échantillon ;
    une première électrode disposée dans la première chambre de réception d'échantillon entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon ;
    une deuxième électrode disposée dans la première chambre de réception d'échantillon entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon ;
    une deuxième chambre de réception d'échantillon qui croise la première chambre de réception d'échantillon entre la première électrode et la deuxième électrode, définissant ainsi une intersection de chambres, et au moins une troisième électrode disposée dans la deuxième chambre de réception d'échantillon.
  2. Bandelette réactive d'analyse électrochimique de la revendication 1 dans laquelle l'intersection de chambres se situe entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon.
  3. Bandelette réactive d'analyse électrochimique de la revendication 1, comprenant en outre :
    une couche substrat électriquement isolante ;
    une couche de conducteur à motif disposée pardessus la couche substrat électriquement isolante, la couche conductrice à motif comportant les première, deuxième et au moins troisième électrodes ;
    une couche de réactif enzymatique disposée pardessus la couche de conducteur à motif ;
    une couche d'entretoise à motif ;
    une couche hydrophile ; et
    une couche supérieure,
    dans laquelle la couche substrat électriquement isolante, la couche d'entretoise à motif, la couche hydrophile et la couche supérieure définissent essentiellement la première chambre de réception d'échantillon, la deuxième chambre de réception d'échantillon, la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon.
  4. Bandelette réactive d'analyse électrochimique de la revendication 1 dans laquelle la première électrode et la deuxième électrode sont configurées pour la détermination de l'hématocrite d'un échantillon de fluide corporel dans la première chambre de réception d'échantillon.
  5. Bandelette réactive d'analyse électrochimique de la revendication 1 dans laquelle l'au moins une troisième électrode comporte une première électrode de travail, une deuxième électrode de travail et une contre-électrode/électrode de référence.
  6. Bandelette réactive d'analyse électrochimique de la revendication 5 dans laquelle la première électrode de travail, une deuxième électrode de travail et une contre-électrode/électrode de référence sont configurées pour la détermination d'un analyte d'un échantillon de fluide corporel dans la deuxième chambre de réception d'échantillon.
  7. Procédé de détermination d'un analyte dans un échantillon de fluide corporel, le procédé comprenant :
    l'application d'un échantillon de fluide corporel à l'une ou l'autre d'une première ouverture d'application d'échantillon et d'une deuxième ouverture d'application d'échantillon d'une première chambre de réception d'échantillon d'une bandelette réactive d'analyse électrochimique de telle sorte que l'échantillon de fluide corporel appliqué remplisse la première chambre de réception d'échantillon et une deuxième chambre de réception d'échantillon de la bandelette réactive d'analyse électrochimique,
    la mesure d'une première réponse de la bandelette réactive d'analyse électrochimique qui dépend de l'échantillon de fluide corporel dans la deuxième chambre de réception d'échantillon ; et
    la détermination de l'analyte en fonction de la réponse électrochimique mesurée ;
    dans lequel la bandelette réactive est configurée de telle sorte qu'une première électrode est disposée dans la première chambre de réception d'échantillon entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon, et une deuxième électrode est disposée dans la première chambre de réception d'échantillon entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon ; et
    la deuxième chambre de réception d'échantillon croise la première chambre de réception d'échantillon entre la première électrode et la deuxième électrode, définissant ainsi une intersection de chambres, et au moins une troisième électrode est disposée dans la deuxième chambre de réception d'échantillon.
  8. Procédé de la revendication 7 comportant en outre :
    la mesure d'une deuxième réponse de la bandelette réactive d'analyse qui dépend de l'échantillon de fluide corporel dans la première chambre de réception d'échantillon capillaire ; et
    la détermination d'une caractéristique de l'échantillon de fluide corporel en fonction de la deuxième réponse mesurée.
  9. Bandelette réactive d'analyse électrochimique de la revendication 6 ou procédé de la revendication 7 ou la revendication 8 dans lesquels l'échantillon de fluide corporel est du sang total.
  10. Bandelette réactive d'analyse électrochimique de la revendication 6 ou procédé de la revendication 9 dans lesquels l'analyte est le glucose.
  11. Procédé de la revendication 7 dans lequel la caractéristique est l'hématocrite.
  12. Procédé de la revendication 7 dans lequel l'intersection de chambres se situe entre la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon.
  13. Bandelette réactive d'analyse électrochimique de la revendication 1 ou procédé de la revendication 12 dans lesquels la première chambre de réception d'échantillon capillaire et la deuxième chambre de réception d'échantillon capillaire sont disposées dans une configuration essentiellement en forme de T.
  14. Bandelette réactive d'analyse électrochimique de la revendication 1 ou procédé de la revendication 13 dans lesquels la configuration en forme de T est symétrique autour d'un axe vertical de la bandelette réactive d'analyse électrochimique.
  15. Bandelette réactive d'analyse électrochimique de la revendication 1 ou procédé de la revendication 7 dans lesquels la deuxième chambre de réception d'échantillon a une entrée d'échantillon, et
    dans lesquels l'intersection de chambres est configurée comme l'entrée d'échantillon de la deuxième chambre de réception d'échantillon.
  16. Bandelette réactive d'analyse électrochimique de la revendication 1 ou procédé de la revendication 7 dans lesquels la première ouverture d'application d'échantillon et la deuxième ouverture d'application d'échantillon sont sur des bords latéraux opposés de la bandelette réactive d'analyse électrochimique.
  17. Bandelette réactive d'analyse électrochimique de la revendication 1 ou procédé de la revendication 12 dans lesquels la première chambre de réception d'échantillon est une chambre de réception d'échantillon sans réactif.
EP13733247.4A 2012-06-21 2013-06-20 Bandelette analytique électrochimique à chambres réceptrices d'échantillons en intersection Active EP2864494B1 (fr)

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US13/529,890 US8877023B2 (en) 2012-06-21 2012-06-21 Electrochemical-based analytical test strip with intersecting sample-receiving chambers
PCT/EP2013/062950 WO2013190072A1 (fr) 2012-06-21 2013-06-20 Bandelette analytique électrochimique à chambres réceptrices d'échantillons en intersection

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EP (1) EP2864494B1 (fr)
JP (1) JP2015520392A (fr)
KR (1) KR20150048702A (fr)
CN (1) CN104379759B (fr)
AU (1) AU2013279297B2 (fr)
BR (1) BR112014031835A2 (fr)
CA (1) CA2876927C (fr)
ES (1) ES2672729T3 (fr)
HK (1) HK1209796A1 (fr)
IN (1) IN2014DN10436A (fr)
RU (1) RU2646493C2 (fr)
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US9897566B2 (en) 2014-01-13 2018-02-20 Changsha Sinocare Inc. Disposable test sensor
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RU2646493C2 (ru) 2018-03-05
ES2672729T3 (es) 2018-06-15
AU2013279297B2 (en) 2018-12-06
CA2876927C (fr) 2020-09-08
US20130341208A1 (en) 2013-12-26
JP2015520392A (ja) 2015-07-16
BR112014031835A2 (pt) 2017-06-27
HK1209796A1 (en) 2016-04-08
WO2013190072A1 (fr) 2013-12-27
CN104379759A (zh) 2015-02-25
KR20150048702A (ko) 2015-05-07
RU2015101705A (ru) 2016-08-10
CA2876927A1 (fr) 2013-12-27
IN2014DN10436A (fr) 2015-08-21
EP2864494A1 (fr) 2015-04-29
CN104379759B (zh) 2016-11-16
US8877023B2 (en) 2014-11-04

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