EP2834367A4 - Sample preparation for flow cytometry - Google Patents

Sample preparation for flow cytometry

Info

Publication number
EP2834367A4
EP2834367A4 EP13773119.6A EP13773119A EP2834367A4 EP 2834367 A4 EP2834367 A4 EP 2834367A4 EP 13773119 A EP13773119 A EP 13773119A EP 2834367 A4 EP2834367 A4 EP 2834367A4
Authority
EP
European Patent Office
Prior art keywords
flow cytometry
sample preparation
sample
preparation
cytometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13773119.6A
Other languages
German (de)
French (fr)
Other versions
EP2834367A1 (en
Inventor
Xiao Li
Yongqiang Zhang
Kenneth Anthony Kopher
John D Mantlo
William Alfred Pope
Song Shi
Axel A Yup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of EP2834367A1 publication Critical patent/EP2834367A1/en
Publication of EP2834367A4 publication Critical patent/EP2834367A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Plural Heterocyclic Compounds (AREA)
EP13773119.6A 2012-04-05 2013-04-04 Sample preparation for flow cytometry Withdrawn EP2834367A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261620823P 2012-04-05 2012-04-05
PCT/US2013/035291 WO2013152203A1 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Publications (2)

Publication Number Publication Date
EP2834367A1 EP2834367A1 (en) 2015-02-11
EP2834367A4 true EP2834367A4 (en) 2016-03-23

Family

ID=49301050

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13773119.6A Withdrawn EP2834367A4 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Country Status (7)

Country Link
EP (1) EP2834367A4 (en)
JP (1) JP6401146B2 (en)
CN (1) CN104379760B (en)
AU (1) AU2013243378B2 (en)
CA (1) CA2869472C (en)
IN (1) IN2014DN08957A (en)
WO (1) WO2013152203A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9933341B2 (en) 2012-04-05 2018-04-03 Becton, Dickinson And Company Sample preparation for flow cytometry
ES2715687T3 (en) * 2013-03-13 2019-06-05 Becton Dickinson Co A method to improve the analysis of microorganisms in complex matrices
CN104677809B (en) * 2013-11-29 2018-01-02 中国人民解放军第二军医大学 Cryptococcus viability detection kit and detection method
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN107643238A (en) * 2017-09-19 2018-01-30 中山大学附属第医院 Method for quantitatively detecting concentration of blood circulation particles
EP3830547B1 (en) * 2018-08-01 2023-11-29 Sartorius BioAnalytical Instruments, Inc. Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes
JP2022525322A (en) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド Methods and systems for time-gate fluorescence-based detection

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030013849A1 (en) * 1999-10-29 2003-01-16 Ward William W. Renilla reniformis green fluorescent protein
RU2172512C1 (en) * 2000-02-01 2001-08-20 Закрытое акционерное общество Научно-производственное объединение "ФоМос" Colored spectrum-zone silver halide photographic material
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
DK1364044T3 (en) * 2000-11-17 2012-05-07 Biomoda Inc Compositions and Methods for Detecting Precancerous Conditions in Cell and Tissue Samples Using 5,10,15,20-Tetrakis (Carboxyphenyl) Porphine
US7205100B2 (en) * 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
EP2582838B1 (en) * 2010-06-21 2014-07-16 Siemens Healthcare Diagnostics Inc. Soluble quencher to reduce background in qpcr assays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
A. PIANETTI ET AL: "Determination of the Viability of Aeromonas hydrophila in Different Types of Water by Flow Cytometry, and Comparison with Classical Methods", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, 1 December 2005 (2005-12-01), US, pages 7948 - 7954, XP055223542, ISSN: 0099-2240, DOI: 10.1128/AEM.71.12.7948-7954.2005 *
BARBESTI S ET AL: "TWO AND THREE-COLOR FLUOROESCENCE FLOW CYTOMETRIC ANALYSIS OF IMMUNOIDENTIFIED VIABLE BACTERIA", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 40, no. 3, 1 July 2000 (2000-07-01), pages 214 - 218, XP009042629, ISSN: 0196-4763, DOI: 10.1002/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO;2-M *
HUBERT ZIPPER ET AL: "Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD, vol. 32, no. 12, 1 January 2004 (2004-01-01), pages e103 - 1, XP009140291, ISSN: 0305-1048 *
JUNG KYU CHOI ET AL: "3,3'-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 12, no. 12, 16 November 2011 (2011-11-16), pages 8052 - 8062, XP055223668, DOI: 10.3390/ijms12118052 *
M. BERNEY: "Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SODIS)", MICROBIOLOGY, vol. 152, no. 6, 1 June 2006 (2006-06-01), GB, pages 1719 - 1729, XP055223549, ISSN: 1350-0872, DOI: 10.1099/mic.0.28617-0 *
MONIS P T ET AL: "Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis", ANALYTICAL BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 340, no. 1, 1 May 2005 (2005-05-01), pages 24 - 34, XP022593917, ISSN: 0003-2697, [retrieved on 20050328], DOI: 10.1016/J.AB.2005.01.046 *
S. ALLEGRA ET AL: "Use of Flow Cytometry To Monitor Legionella Viability", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 24, 10 October 2008 (2008-10-10), US, pages 7813 - 7816, XP055223546, ISSN: 0099-2240, DOI: 10.1128/AEM.01364-08 *
See also references of WO2013152203A1 *

Also Published As

Publication number Publication date
IN2014DN08957A (en) 2015-05-22
AU2013243378A1 (en) 2014-10-30
JP6401146B2 (en) 2018-10-03
CA2869472A1 (en) 2013-10-10
JP2015514403A (en) 2015-05-21
AU2013243378B2 (en) 2019-04-11
WO2013152203A1 (en) 2013-10-10
CN104379760B (en) 2018-03-16
CN104379760A (en) 2015-02-25
CA2869472C (en) 2017-07-11
EP2834367A1 (en) 2015-02-11

Similar Documents

Publication Publication Date Title
HUS2400016I1 (en) Anti-fcrn antibodies
IL264558A (en) Anti-il-23p19 antibodies
EP2861956A4 (en) Flow cytometer
EP2834367A4 (en) Sample preparation for flow cytometry
HK1204571A1 (en) Protease-regulated antibodies
HK1210192A1 (en) Anti-siglec-15 antibodies siglec-15
SG11201404780VA (en) Sorting flow cytometer
EP2859332A4 (en) Fluorescence flow cytometry
HK1214650A1 (en) Sample analysis by mass cytometry
EP2844976A4 (en) Single magnet fluid densitometer
PT3026417T (en) Container for specimen preparation
EP2867374A4 (en) Rapid assays for t-cell activation by rna measurements using flow cytometry
EP2903489A4 (en) Mixing chamber for two fluid constituents
HK1210186A1 (en) Anti-h7cr antibodies h7cr
EP2906913A4 (en) Flow measurement
PL2935330T3 (en) Anti-notch3 antibodies
PL2898308T3 (en) Sample probe inlet flow system
EP2935445A4 (en) Composition comprising particulate flow aid
PL2626134T3 (en) Pipette
GB2499995B (en) Flow meter
AU2012904449A0 (en) Flow Measurement
GB201213045D0 (en) Flow exiter
GB201204631D0 (en) Flow monitor for hosepipes
GB201204788D0 (en) Flow divider

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20141031

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAX Request for extension of the european patent (deleted)
RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/02 20060101AFI20151027BHEP

Ipc: C12Q 1/68 20060101ALI20151027BHEP

Ipc: G01N 33/48 20060101ALI20151027BHEP

Ipc: C12M 1/34 20060101ALI20151027BHEP

RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20160222

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/68 20060101ALI20160216BHEP

Ipc: C12M 1/34 20060101ALI20160216BHEP

Ipc: G01N 33/48 20060101ALI20160216BHEP

Ipc: C12Q 1/02 20060101AFI20160216BHEP

17Q First examination report despatched

Effective date: 20170130

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20171222