EP2762560A4 - Method for inducing orexin neuron - Google Patents

Method for inducing orexin neuron

Info

Publication number
EP2762560A4
EP2762560A4 EP12837508.6A EP12837508A EP2762560A4 EP 2762560 A4 EP2762560 A4 EP 2762560A4 EP 12837508 A EP12837508 A EP 12837508A EP 2762560 A4 EP2762560 A4 EP 2762560A4
Authority
EP
European Patent Office
Prior art keywords
inducing
orexin neuron
orexin
neuron
inducing orexin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12837508.6A
Other languages
German (de)
French (fr)
Other versions
EP2762560A1 (en
Inventor
Kunio Shiota
Shintaro Yagi
Koji Hayakawa
Mitsuko Hirosawa-Takamori
Daisuke Arai
Keiji Hirabayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokyo NUC
Original Assignee
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Tokyo NUC filed Critical University of Tokyo NUC
Publication of EP2762560A1 publication Critical patent/EP2762560A1/en
Publication of EP2762560A4 publication Critical patent/EP2762560A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2864—Sleep disorders

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Neurosurgery (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
EP12837508.6A 2011-09-29 2012-09-28 Method for inducing orexin neuron Withdrawn EP2762560A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161540601P 2011-09-29 2011-09-29
PCT/JP2012/075137 WO2013047773A1 (en) 2011-09-29 2012-09-28 Method for inducing orexin neuron

Publications (2)

Publication Number Publication Date
EP2762560A1 EP2762560A1 (en) 2014-08-06
EP2762560A4 true EP2762560A4 (en) 2015-05-20

Family

ID=47995796

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12837508.6A Withdrawn EP2762560A4 (en) 2011-09-29 2012-09-28 Method for inducing orexin neuron

Country Status (3)

Country Link
EP (1) EP2762560A4 (en)
JP (1) JP6120223B2 (en)
WO (1) WO2013047773A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6323886B2 (en) * 2014-05-16 2018-05-16 国立大学法人 東京大学 Depressant

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007069666A1 (en) * 2005-12-13 2007-06-21 Kyoto University Nuclear reprogramming factor
EP2314671A1 (en) * 2008-06-06 2011-04-27 Riken Method for culture of stem cell

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4044630B2 (en) * 1996-05-16 2008-02-06 雪印乳業株式会社 Brain function improver
JPH10182685A (en) 1996-12-26 1998-07-07 Ngk Insulators Ltd Production of n-acetylmannosamine
WO2000007602A1 (en) * 1998-08-06 2000-02-17 The Johns Hopkins University School Of Medicine Compounds for altering cell surface sialic acids and methods of use therefor
JP4395645B2 (en) 1999-09-10 2010-01-13 ジャパン・フード＆リカー・アライアンス株式会社 Method for producing N-acetylmannosamine
US7666673B2 (en) 2003-04-15 2010-02-23 Kyoto University Method of growing sperm stem cells in vitro, sperm stem cells grown by the method, and medium additive kit to be used in growing sperm stem cells in vitro
ES2298052B1 (en) * 2006-08-03 2009-09-28 Bioiberica S.A. PROCEDURE FOR THE PROLIFERATION OF CELLS.
MX2010013192A (en) * 2008-06-16 2010-12-17 Hoffmann La Roche Heteroaromatic monoamides as orexinin receptor antagonists.
JP5553168B2 (en) 2008-09-04 2014-07-16 国立大学法人 東京大学 Improvement agent of brain function decline
JP5557243B2 (en) 2010-02-26 2014-07-23 国立大学法人 東京大学 Sleep improver

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007069666A1 (en) * 2005-12-13 2007-06-21 Kyoto University Nuclear reprogramming factor
EP2314671A1 (en) * 2008-06-06 2011-04-27 Riken Method for culture of stem cell

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEMELLI RICHARD M ET AL: "Narcolepsy in orexin knockout mice: Molecular genetics of sleep regulation", CELL, CELL PRESS, US, vol. 98, no. 4, 20 August 1999 (1999-08-20), pages 437 - 451, XP002175980, ISSN: 0092-8674, DOI: 10.1016/S0092-8674(00)81973-X *
HAYAKAWA KOJI ET AL: "Epigenetic Switching by the Metabolism-sensing Factors in the Generation of Orexin Neurons from Mouse Embryonic Stem Cells", THE JOURNAL OF BIOLOGICAL CHEMISTRY 14 JUN 2013, vol. 288, no. 24, 14 June 2013 (2013-06-14) - 26 April 2013 (2013-04-26), pages 17099 - 17110, XP055179950, ISSN: 1083-351X *
KESSLER B A ET AL: "Age-related loss of orexin/hypocretin neurons", NEUROSCIENCE, NEW YORK, NY, US, vol. 178, 15 January 2011 (2011-01-15), pages 82 - 88, XP028184247, ISSN: 0306-4522, [retrieved on 20110122], DOI: 10.1016/J.NEUROSCIENCE.2011.01.031 *
LIN LING ET AL: "The sleep disorder canine narcolepsy is caused by a mutation in the hypocretin (orexin) receptor 2 gene", CELL, CELL PRESS, US, vol. 98, no. 3, 6 August 1999 (1999-08-06), pages 365 - 376, XP002153571, ISSN: 0092-8674, DOI: 10.1016/S0092-8674(00)81965-0 *
N. TSUJINO ET AL: "Orexin/Hypocretin: A Neuropeptide at the Interface of Sleep, Energy Homeostasis, and Reward System", PHARMACOLOGICAL REVIEWS, vol. 61, no. 2, 1 June 2009 (2009-06-01), pages 162 - 176, XP055180000, ISSN: 0031-6997, DOI: 10.1124/pr.109.001321 *
See also references of WO2013047773A1 *

Also Published As

Publication number Publication date
EP2762560A1 (en) 2014-08-06
JP6120223B2 (en) 2017-04-26
WO2013047773A1 (en) 2013-04-04
JPWO2013047773A1 (en) 2015-03-30

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