EP2692232B1 - Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes - Google Patents

Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes Download PDF

Info

Publication number
EP2692232B1
EP2692232B1 EP12763915.1A EP12763915A EP2692232B1 EP 2692232 B1 EP2692232 B1 EP 2692232B1 EP 12763915 A EP12763915 A EP 12763915A EP 2692232 B1 EP2692232 B1 EP 2692232B1
Authority
EP
European Patent Office
Prior art keywords
plants
infection
vanillin
hydroalcoholic
row
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP12763915.1A
Other languages
German (de)
English (en)
Other versions
EP2692232A4 (fr
EP2692232A1 (fr
Inventor
Rosa Maria MATEO BERNAL
Rebeca COBOS ROMÁN
Penélope GARCÍA ANGULO
Alberto Alonso Monroy
Maria Luisa ÁLVAREZ RODRíGUEZ
Silvia SEVILLANO NISTAL
José Manuel ÁLAVARE PÉREZ
José Luis ACEBES ARRANZ
Enrrique GARZÓN JIMENO
Juan José RUBIO COQUE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bodegas Vega Sicilia SA
Original Assignee
Bodegas Vega Sicilia SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bodegas Vega Sicilia SA filed Critical Bodegas Vega Sicilia SA
Publication of EP2692232A1 publication Critical patent/EP2692232A1/fr
Publication of EP2692232A4 publication Critical patent/EP2692232A4/fr
Application granted granted Critical
Publication of EP2692232B1 publication Critical patent/EP2692232B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives

Definitions

  • the purpose of the invention refers to the use of natural antifungal agents, applied in combination to prevent the infections by phytopathogenic fungi of pruning wounds, lesions and grafts in grapevine plants.
  • This invention refers to the use in combination, of three natural compounds/extracts that separately have antifungal properties and that by combining them show very high effectiveness in avoiding infection through pruning wounds, lesions and grafts of grapevines by phytopathogenic fungi responsible for grapevine trunk diseases, avoiding the death of the plant due to this type of pathogens.
  • the different compounds/extracts and their mixture has shown in trials conducted on culture medium plates a high capacity for inhibiting the growth of the following phytopathogenic fungi involved in the development of grapevine trunk diseases: Botryosphaeria dothidea, Cylindrocarpon macrodidymum ( Neonectria macrodidyma), Diplodia seriata, Eutypa lata, Phaeomoniella chlamydospora, Phaeoacremonium aleophylum and Phomopsis viticola.
  • a mix of these compounds/extracts is especially recommended as an antifungal agent to avoid the infection of grapevines through pruning wounds, grafts or other type of lesions.
  • the petitioner does not know of precedents of inventions referring to the use of some natural antifungal agents, applied in combination, to prevent the infection of pruning wounds, grafts and lesions in grapevine plants by phytopathogenic fungi.
  • grapevine trunk diseases or grapevine decline encompasses a set of pathologies produced as a consequence of the infection of the plant by a series of phytopathogenic fungi.
  • the infection of the plants is an unsolved problem that generally begins through the root or more frequently through pruning wounds or other type of lesions produced during the tillage of the vineyard or through attack by insects.
  • the grapevine trunk diseases are a serious problem for the world's wine-producing industry.
  • the wine-producing industry is very important for the European Union. It represents 47% of the world's wine-producing surface area and 60% of the worldwide production of wine.
  • the principal producers on the world level are France, Italy, Spain, among the three producing more than 50% of the worldwide production and cornering 53.8% of the world's wine exports.
  • the Spanish wine-producing sector is of great importance because of the economic value that it generates, for the extension that it occupies, as well as for the good image that it produces of Spain internationally. Spain dedicates 1.2 million hectares to the growing of grapes (of which 97.4% is used for producing wine, 2% for table grapes, 0.3% for the elaboration of raisins and the remaining 0.3% to nurseries). It is the country with the largest extension of vineyards in the world. In addition, the grapevines occupy third place in extension of Spanish crops, following cereals and olive trees.
  • the grapevine trunk diseases are probably at present the greatest threat worldwide faced by the wine producers and grapevine nurseries for various reasons:
  • the purpose of the invention is related to the use of chitosan oligosaccharide, garlic extract (Allium sativum L.) and vanillin (4-hydroxy-3 methoxybenzaldehyde), used in combination for the treatment of pruning wounds of the grapevine. These compounds/extracts were selected after testing numerous natural compounds/extracts with antifungal properties.
  • Trial 1 To determine the inhibiting effect of the chitosan, hydroalcoholic garlic extract and vanillin on the growth of phytopathogenic fungi responsible for the grapevine trunk diseases determined by means of plate assays.
  • Botryosphaeria dothidea Cylindrocarpon macrodidymum ( Neonectria macrodidyma), Diplodia seriata , Eutypa lata, Phaeomoniella chlamydospora, Phaeoacremonium aleophylum and Phomopsis viticola.
  • the trials consisted of inoculating these fungi in the centre of the PDA (potato dextrose agar) culture medium plates containing the antifungal compound/extract.
  • the pates were incubated at 25°C for 5-30 days, depending on the species of fungus, and the radial growth of the colony was measured every 24 hours.
  • the radial growth values obtained were compared with the radial growth data of the same fungus growing on PDA medium plates that did not contain the antifungal agent.
  • Trial 2 To determine the inhibiting effect of chitosan, hydroalcoholic garlic extract and vanillin on the growth of phytopathogenic fungi responsible for determined grapevine trunk diseases by means of tests carried out on grapevine shoots.
  • the tests were conducted with grapevine shoots of 7-10 cm in length.
  • the shoots were sterilised in autoclave to eliminate any fungus or bacteria that might interfere in the test.
  • the test consisted of making a small opening in the shoot of around 4 mm in diameter and depth using a scalpel.
  • a fragment of agar of around 3 mm of diameter, carrier of the phytopathogenic fungus was inserted.
  • the hole was then sealed with plastic paper and the shoot was introduced in a sterile plastic tube in the bottom of which we introduced sterile cotton soaked in water to preserve moist conditions in the interior of the tube that would favour the development of the fungus.
  • the tube was then incubated at 25°C for 7-30 days, depending on the fungus tested. We then proceeded to analyse the shoots, cutting two slices of the trunk 1 cm above and below the inoculation point. From each slice 7 woodchips were extracted which were deposited on PDA medium plates. After incubation at 25°C to permit the growth of the fungus, the results of the inhibition of the growth of the pathogen by the antifungal agent, which was expressed as the ratio (percentage) of woodchips from which the pathogen was developed in the case of the shoots treated with antifungal agent, compared to the number of wood chips that developed the growth of the pathogen in the case of the shoots infected but not treated with the antifungal agent. Each test was conducted in triplicate, using batches of three control shoots and three shoots treated with the antifungal agent.
  • Trial 3 To determine the protective effect of the chitosan, hydroalcoholic garlic extract and vanillin on grapevine plants artificially infected with phytopathogenic fungi.
  • the antifungal agents were applied with a brush on the pruning wounds.
  • the chitosan oligosaccharide is prepared by means of dilution in distilled water of an initial concentration of 100 mg/ml. It is applied on the pruning wounds at a concentration of 10-25 mg/ml.
  • the hydroalcoholic garlic extract is prepared by means of homogenisation of peeled garlic cloves at an initial concentration of 30% (p/v) in ethanol at 10%.
  • the homogenisation is achieved by grinding them with a grinder or similar until obtaining a homogenous and fine suspension.
  • the suspension is then incubated for 2 hours at 4°C with stirring for a maximum extraction of the active ingredients.
  • the suspension can be filtered in order to eliminate solid materials.
  • the application on the pruning wounds is done at a concentration of 7-10% (p/v) by means of dilution of the initial suspension in distilled water.
  • the vanillin is prepared by means of dilution in methanol at an initial concentration of 200 mg/ml.
  • the application on the pruning wounds is done at a concentration of 3-5 mg/ml, by means of dilution of the original dilution in ethanol at 10% (v/v) or water.
  • chitosan oligosaccharide hydroalcoholic garlic extract and vanillin is prepared from the dilutions or concentrated suspensions of the products separately and is applied on the pruning wounds with a composition of chitosan (20-25 mg/ml)-hydroalcoholic garlic extract (8-10%)-vanillin (3-4 mg/ml).

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Claims (4)

  1. Utilisation d'une combinaison d'antifongiques naturels pour prévenir l'infection de lésions, greffes et plaies de tailles causée à la vigne par des champignons phytopathogènes responsables de maladies du bois, caractérisée par le fait qu'elle contient du chitosan oligosaccharide, de l'extrait hydro-alcoolique d'ail et de la vanille.
  2. Una combinaison d'antifongiques naturels pour prévenir l'infection de lésions, greffes et plaies de taille causée à la vigne par des champignons phytopathogènes responsables de maladies du bois, caractérisée par le fait qu'elle contient du chitosan oligosaccharide, de l'extrait hydro-alcoolique d'ail et de la vanille.
  3. Méthode d'obtention d'un composé d'antifongiques naturels pour prévenir l'infection de lésions, greffes et plaies de taille causée à la vigne par des champignons phytopathogènes responsables des maladies du bois, parce que :
    a) le chitosan oligosaccharide est préparé avec une solution d'eau distillée dans une concentration initiale 100 mg/ml ;
    b) l'extrait hydro-alcoolique d'ail est préparé avec l'homogénéisation de dents d'ail pelés dans une concentration initiale de 30% (p/v) dans de l'éthanol à 10% ;
    c) la vanille est préparée avec une solution de méthanol dans une concentration initiale de 200 mg/ml ;
    d) le composé est préparé avec une combinaison de chitosan oligosaccharide, dans une concentration de 10-25 mg/ml et d'extrait hydro-alcoolique d'ail dans une concentration de 7-10% (p/v) à travers la dissolution de la suspension initiale dans de l'eau distillée et de la vanille dans une concentration de 3-5 mg/ml (par dissolution de la dissolution initiale dans de l'éthanol à 10% (v/v) ou de l'eau).
  4. Utilisation d'un composé ou d'une combinaison d'antifongiques naturels pour prévenir l'infection de lésions, greffes et plaies de taille causée à la vigne par des champignons phytopathogènes responsables de maladies du bois, suivant la revendication 1, de préférence dans une proportion de chitosan oligosaccharide (25 mg/ml), de l'extrait hydro-alcoolique d'ail (10%) et de vanille (4 mg/ml).
EP12763915.1A 2011-03-28 2012-03-13 Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes Active EP2692232B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES201130461A ES2389343B2 (es) 2011-03-28 2011-03-28 Utilización de antifúngicos naturales para prevenir la infección de lesiones, injertos y heridas de poda en plantas leñosas por hongos fitopatógenos.
PCT/ES2012/000057 WO2012131117A1 (fr) 2011-03-28 2012-03-13 Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes

Publications (3)

Publication Number Publication Date
EP2692232A1 EP2692232A1 (fr) 2014-02-05
EP2692232A4 EP2692232A4 (fr) 2014-10-08
EP2692232B1 true EP2692232B1 (fr) 2015-10-28

Family

ID=46929520

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12763915.1A Active EP2692232B1 (fr) 2011-03-28 2012-03-13 Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes

Country Status (5)

Country Link
EP (1) EP2692232B1 (fr)
ES (2) ES2389343B2 (fr)
HU (1) HUE026550T2 (fr)
PT (1) PT2692232E (fr)
WO (1) WO2012131117A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039468A (zh) * 2012-12-29 2013-04-17 青岛中达农业科技有限公司 一种壳寡糖与丙森锌的植保用组合物
ES2543363B2 (es) * 2015-04-01 2016-03-15 Viveros Villanueva Vides, S.L. Producto para el control de hongos fitopatógenos causantes de enfermedades de madera de vid y procedimiento para su aplicación en injertos de vid
CN107518060A (zh) * 2017-07-20 2017-12-29 江苏师范大学 一种壳寡糖制剂及其在甘薯采后黑斑病防治中的应用
CN109452319B (zh) * 2018-12-19 2021-04-23 江西省农业科学院植物保护研究所 一种高效防治芦笋茎枯病的组合物及其应用
CN109566620B (zh) * 2018-12-29 2021-06-22 海南正业中农高科股份有限公司 一种杀菌组合物及其应用和杀菌剂

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5549739A (en) * 1993-11-26 1996-08-27 Nippon Suisan Kaisha, Ltd. Wood modifier composition
US20080260876A1 (en) * 2005-04-01 2008-10-23 Nicole Benhamou Garlic Extract Compositions, Uses And Preparations Thereof
ES2328322A1 (es) * 2007-10-08 2009-11-11 Timac Agro España, S.A. Composiciones de origen natural para tratar fisiopatias y enfermedades producidas por agentes patogenos de frutas y hortalizas, despues de su cosecha, y metodo para su aplicacion.

Also Published As

Publication number Publication date
ES2389343B2 (es) 2013-06-06
ES2556054T3 (es) 2016-01-12
EP2692232A4 (fr) 2014-10-08
PT2692232E (pt) 2016-02-10
HUE026550T2 (en) 2016-06-28
EP2692232A1 (fr) 2014-02-05
WO2012131117A8 (fr) 2016-05-26
WO2012131117A1 (fr) 2012-10-04
ES2556054T8 (es) 2016-04-13
ES2389343A1 (es) 2012-10-25

Similar Documents

Publication Publication Date Title
Fontaine et al. Grapevine trunk diseases. A review
Halleen et al. An integrated strategy for the proactive management of grapevine trunk disease pathogen infections in grapevine nurseries
EP2692232B1 (fr) Utilisation d'antifongiques naturels pour prévenir l'infection de lésions, de greffes et des plaies d'élagage dans des plantes ligneuses par des champignons phytopathogènes
Saeed et al. Assessment of nematicidal potential of Cannabis sativa and Azadirachta indica in the management of root-knot nematode (Meloidogyne javanica) on peach.
Brown et al. DISEASE DURING PROPAGATION OF
Anikwe et al. Dieback of cocoa (Theobroma cacao L.) plant tissues caused by the brown cocoa mirid Sahlbergella singularis Haglund (Hemiptera: Miridae) and associated pathogenic fungi
Yildiz et al. Molecular characterization of black foot disease pathogens in grapevine nurseries and evaluation of some fungicides for control of the most virulent isolates
Ram et al. Stone fruit diseases and their management
Anusha et al. Effect of hexanal on mycelial growth and spore germination of Colletotrichum gloeosporioides and Lasiodiplodia theobromae of mango.
Srivastava et al. Diseases of apples and their management
Garcia A comparative study on the antifungal effects of tamarind (Tamarindus indica) and garlic (Allium sativum) extracts on banana anthracnose
Haq et al. Diseases of Dalbergia sissoo: Etiology and integrated management of economically important diseases
Kalita et al. Guava (Psidium Guajava): Key Diseases and Their Management
Langa et al. Effectiveness of different formulations of moringa extracts against postharvest papaya diseases
Aidoo Bioactivity of Zingiber officinale and Piper nigrum plant extracts in controlling post-harvest white yam (Dioscorea rotundata) tuber rot fungi
US20170258096A1 (en) Compositions and methods to treat plant pathogens
Pandey et al. Shyam Chandra Roy Chandel, Anuj Rani, Rashmi Singh, and Ravi Shankar Mishra
Rasool et al. Major Diseases and Their Management
Kumbhar Studies on Eco Friendly Approaches for the Integrated Management of Fungal Diseases of Onion Allium cepa L
Misra 15 Diseases
Ciesla Pests and diseases of neem
WO2021114002A1 (fr) Souche endophyte
WO2023169782A1 (fr) Composition nématicide pour la lutte contre les nématodes parasites des plantes
Rawal Present scenario of important fungal diseases of grapes and their management
Shakywar et al. Diseases of Rose Crop and Their Management

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20131028

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602012012006

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: A01N0063000000

Ipc: A01N0063020000

A4 Supplementary search report drawn up and despatched

Effective date: 20140909

RIC1 Information provided on ipc code assigned before grant

Ipc: A01N 35/04 20060101ALI20140903BHEP

Ipc: A01N 63/02 20060101AFI20140903BHEP

Ipc: A01P 3/00 20060101ALI20140903BHEP

Ipc: A01N 65/42 20090101ALI20140903BHEP

Ipc: A01N 43/16 20060101ALI20140903BHEP

17Q First examination report despatched

Effective date: 20150428

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20150701

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 757415

Country of ref document: AT

Kind code of ref document: T

Effective date: 20151115

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602012012006

Country of ref document: DE

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: BOVARD AG, CH

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2556054

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20160112

REG Reference to a national code

Ref country code: PT

Ref legal event code: SC4A

Free format text: AVAILABILITY OF NATIONAL TRANSLATION

Effective date: 20160113

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20151028

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 5

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 757415

Country of ref document: AT

Kind code of ref document: T

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20160228

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20160128

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

RIN2 Information on inventor provided after grant (corrected)

Inventor name: ALONSO MONROY, ALBERTO

Inventor name: MATEO BERNAL, ROSA MARIA

Inventor name: RUBIO COQUE, JUAN JOSE

Inventor name: ACEBES ARRANZ, JOSE LUIS

Inventor name: GARZON JIMENO, ENRRIQUE

Inventor name: SEVILLANO NISTAL, SILVIA

Inventor name: ALAVAREZ PEREZ, JOSE MANUEL

Inventor name: COBOS ROMAN, REBECA

Inventor name: GARCIA ANGULO, PENELOPE

Inventor name: ALVAREZ RODRIGUEZ, MARIA LUISA

RIN2 Information on inventor provided after grant (corrected)

Inventor name: ALONSO MONROY, ALBERTO

Inventor name: COBOS ROMAN, REBECA

Inventor name: ACEBES ARRANZ, JOSE LUIS

Inventor name: GARZON JIMENO, ENRRIQUE

Inventor name: MATEO BERNAL, ROSA MARIA

Inventor name: SEVILLANO NISTAL, SILVIA

Inventor name: ALVAREZ PEREZ, JOSE MANUEL

Inventor name: ALVAREZ RODRIGUEZ, MARIA LUISA

Inventor name: GARCIA ANGULO, PENELOPE

Inventor name: RUBIO COQUE, JUAN JOSE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20160129

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

REG Reference to a national code

Ref country code: HU

Ref legal event code: AG4A

Ref document number: E026550

Country of ref document: HU

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602012012006

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160331

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20160729

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20160313

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160313

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 7

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20160331

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151028

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602012012006

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: A01N0063020000

Ipc: A01N0063200000

REG Reference to a national code

Ref country code: DE

Ref legal event code: R082

Ref document number: 602012012006

Country of ref document: DE

Representative=s name: DEHNS GERMANY PARTNERSCHAFT MBB, DE

Ref country code: DE

Ref legal event code: R082

Ref document number: 602012012006

Country of ref document: DE

Representative=s name: DEHNSGERMANY PARTNERSCHAFT VON PATENTANWAELTEN, DE

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20230206

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20230302

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20230403

Year of fee payment: 12

Ref country code: CH

Payment date: 20230402

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: HU

Payment date: 20240307

Year of fee payment: 13

Ref country code: DE

Payment date: 20240319

Year of fee payment: 13

Ref country code: PT

Payment date: 20240221

Year of fee payment: 13

Ref country code: GB

Payment date: 20240221

Year of fee payment: 13