EP2637788A1 - Dispositif de filtration du sang - Google Patents

Dispositif de filtration du sang

Info

Publication number
EP2637788A1
EP2637788A1 EP11781496.2A EP11781496A EP2637788A1 EP 2637788 A1 EP2637788 A1 EP 2637788A1 EP 11781496 A EP11781496 A EP 11781496A EP 2637788 A1 EP2637788 A1 EP 2637788A1
Authority
EP
European Patent Office
Prior art keywords
sample
carrier
cover
region
deformation region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11781496.2A
Other languages
German (de)
English (en)
Inventor
Marc Grolla
Gert Blankenstein
Dirk Osterloh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blankenstein Gert
GROLLA, MARC
Original Assignee
Boehringer Ingelheim Microparts GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Microparts GmbH filed Critical Boehringer Ingelheim Microparts GmbH
Priority to EP11781496.2A priority Critical patent/EP2637788A1/fr
Publication of EP2637788A1 publication Critical patent/EP2637788A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0694—Valves, specific forms thereof vents used to stop and induce flow, backpressure valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Definitions

  • the present invention relates to a device for manipulating, in particular filtration, a liquid sample, in particular blood, according to the preamble of claim 1 or 5.
  • the present invention is concerned with the manipulation or filtration of a liquid sample.
  • a liquid sample This is in particular a biological sample or sample liquid, in particular blood or the like.
  • the present invention relates to the filtration of a particle-containing solution (suspension), such as blood or other human or animal body fluid.
  • the present invention is particularly concerned with fluidic devices containing or forming a microfluidic system.
  • the following statements therefore relate preferably to devices in which act capillary forces and in particular for the function are important or relevant.
  • EP 1 421 993 A1 discloses a device for blood separation, wherein a carrier made of a transport fleece, for example of glass fibers, is provided with a fleece in a blood separation region.
  • the separating fleece forms a filter or a Trerm adopted for the separation of blood components.
  • the blood separation is carried out by capillary forces, where supportive, for example, a negative pressure can be applied. A technical realization for the production however, the negative pressure is not indicated.
  • the separated blood plasma is removed from the transport fleece by pressing.
  • the area of the carrier with the separating fleece can be separated from the other carrier in order to prevent potential contamination of the blood plasma by blood cells.
  • the disadvantage here is that a relatively undefined and slow blood separation and forwarding of the separated blood plasma takes place in a nonwoven.
  • Another disadvantage is that a separate device for squeezing to remove the separated blood plasma is required.
  • the present invention has for its object to provide a device for manipulation, in particular filtration, a liquid sample, such as blood, with an optimized or defined manipulation, in particular filtration, a compact structure of the overall arrangement and / or a defined or simple output the manipulated or filtered sample is enabled.
  • One aspect of the present invention is to provide the device with a conveyor for generating negative pressure and / or overpressure for conveying the sample. This allows a defined or rapid manipulation, in particular filtration, of the sample.
  • the conveyor is preferably arranged on or formed from a carrier which forms a fluidic system for the sample. This allows a very simple and / or compact design.
  • the device preferably has a separating device for the sample, wherein the separating device is arranged on a first region of the carrier.
  • the conveying device is preferably arranged separately therefrom or in a second region of the carrier, wherein the two regions are preferably separable from one another.
  • This second region of the carrier preferably also includes a reservoir for the manipulated or filtered sample.
  • the conveyor is designed both for generating a negative pressure, in particular for effecting, accelerating or supporting the filtration, as well as for generating an overpressure, in particular for outputting the filtered sample.
  • the conveyor is therefore preferably used, for example, both for receiving the sample and for dispensing the sample, so in particular has a dual function.
  • This aspect of the present invention can also be implemented independently of the separability of the first carrier region or with the separating device.
  • a further, likewise independently realizable aspect of the present invention is directed to the conveying direction comprising a deformation region of a cover of the carrier with the fluidic system for the sample, wherein the deformation region is flexibly deformable in order to generate a negative pressure and / or overpressure and / or to promote the sample.
  • This allows a very simple and / or compact design.
  • a very simple operation for example by a finger or a fingertip of an operator or the like., Enables.
  • the deformation region as well as the other cover of the carrier is formed by a film, in particular the depressions, channels or the like formed in the carrier. covering the formation of the fluidic system. This allows a very simple and inexpensive construction.
  • the carrier preferably has a shallow depression or recess to allow deformation of the deformation region into the carrier.
  • the recess or recess is preferably via a channel or the like. with the fluidic system or the sample in connection.
  • the cover or the deformation region can also be formed separately from the other cover and / or plug-like and / or have or form a piston.
  • the cover or the deformation region in turn covers a preferably cylindrical recess or recess of the carrier and is for generating a negative pressure or overpressure in the recess, into and / or out of this - particularly preferably transversely to the surface extension of the carrier or the cover - movable, for example by means of a preferably molded actuating element, such as a tab, eyelet or the like.
  • a very simple actuation of the conveying device in particular via manipulating device or the like, can be carried out or realized particularly automatically or automatically.
  • FIG. 1A is a schematic section of a proposed device according to a first embodiment
  • Fig. 1B is a fragmentary enlargement of Fig. 1A;
  • Fig. 2 is a perspective view of a carrier of the proposed device according to the first embodiment.
  • Fig. 3 is a schematic section of a part of a proposal
  • the sample 2 is a particle-containing solution or suspension. Most preferably it is a biological Sample 2. In the illustrated example, it is in particular blood or other human or animal body fluid. However, other liquids or suspensions or the like. be manipulated or filtered as sample 2.
  • the device 1 particularly preferably serves to filter the sample 2, this is not mandatory. Rather, as an alternative or in addition, any other manipulation can take place, for example mixing with a reagent, a solvent or the like, or other treatment or examination of the sample 2.
  • the device 1 has a carrier 3 which forms or has a fluidic system 4.
  • the fluidic system 4 is at least partially or completely formed by or in the carrier 4, possibly together with a cover 5.
  • the carrier 3 is shown in a schematic perspective view.
  • the device 1 preferably has a cover 5 associated with the carrier 3, which preferably at least partially covers the fluidic system 4.
  • the cover 5 covers recesses, grooves or the like formed in the carrier 3.
  • the cover 5 is preferably at least substantially smooth, flat, elastically deformable, and / or formed like a foil.
  • it is a plastic film or the like.
  • the cover 5 is preferably arranged or applied on a flat side of the carrier 3, for example by gluing, sealing, in particular heat sealing, welding or the like.
  • the device 1 or the carrier 3 or the fluidic system 4 preferably has a receiving channel 4A for the preferably filtered sample 2 or the permeate, a reservoir 4B connected thereto for preferably temporarily receiving the preferably filtered Sample 2 or the permeate and / or a connecting channel 4C, as indicated in the perspective view of FIG. 2.
  • the device 1 preferably has a separation device 6 shown in FIG. 1A for the filtration of the sample 2 or the separation of components or particles from the sample 2.
  • the separating device 6 is preferably arranged on the carrier 3, in particular in a first carrier region 3A, and / or firmly connected thereto.
  • the separating device 6 preferably has a flat filter element 6A, for example a membrane or the like, as the main filter for separating off constituents, such as particles, cells or the like, from the sample 2.
  • the filter element may be formed by or provided with a membrane as described in WO 2009/106331 A2.
  • the separation 6 or filtration is preferably multi-stage or multi-layered in the illustrated embodiment. This is illustrated in more detail in Fig. 1B, which shows an enlargement of Fig. 1A in the region of the separator 6.
  • the separating device 6 has a further, flat filter element 6B as a (first) pre-filter and optionally also an additional, preferably flat filter element 6C as a second pre-filter.
  • the filter elements 6A, 6B and 6C are preferably stacked directly on top of each other or flat on each other.
  • the main filter and the optional prefilters or filter elements 6A, 6B and 6C are preferably housed in a housing 6D of the separator 6.
  • the housing 6D is preferably fixedly attached to the carrier 3 or its carrier region 3A, in particular by gluing, heat-sealing, welding or the like.
  • the cover 5 preferably does not extend under the filter or filter elements 6A, 6B, 6C, but may possibly extend slightly below the housing 6D.
  • the separating device 6 or its filter is additionally applied to the carrier 3.
  • the separator 6 or its filter may also be integrated into the carrier 3, in particular in FIG a corresponding recess or depression may be received in the carrier 3.
  • the main filter and the optional prefilters or filter elements 6A, 6B and 6C are preferably held flat by an optional hold-down device 6E, in particular on or against the carrier 3 or its facing surface.
  • the hold-down 6E engages on the side facing away from the carrier 3 flat side of the filter elements 6A, 6B and 6C, in particular here on the outer filter element 6C, ie on the receiving side of the filter, in particular lattice-like, rib-like or spoke-like or lies on the flat side of the filter , here the outer filter element 6C, that the sample 2 evenly distributed on the filter (here the filter element 6C) and / or can easily penetrate it in a large area.
  • the hold-down device 6E is, for example, clamped or otherwise received in the housing 6D.
  • other constructive solutions are possible to suitably build up, bias, hold and / or secure the filter or filter elements 6A, 6B and 6C.
  • the sample 2 already taken in the illustration according to FIG. 11, for example a drop of blood can be distributed uniformly on the filter or the upper filter element 6C and into the filter or here the filter elements 6C, 6B and 6A penetrate in sequence.
  • the separating device 6 or the housing 6D or the sample receptacle formed therefrom can preferably be covered by a cover 6F.
  • Fig. 1 shows the device 1 and separator 6 with attached lid 6F.
  • the separating device 6 or the housing 6D is preferably provided with a ventilation, which is formed here by a ventilation opening 6G, for example in the lid 6F.
  • a ventilation opening 6G is formed here by a ventilation opening 6G, for example in the lid 6F.
  • the fineness of the filter elements 6A, 6B and 6C or the pore size preferably increases toward or away from the carrier 3.
  • the main filter or the filter element 6A filters out the smallest particles, cells or the like which are to be separated from the sample 2.
  • the sample 2 is preferably (also) absorbed by capillary forces in the filter and / or filtered or passed through the filter.
  • the filtered sample 2 or the permeate is taken up and discharged by the carrier 3 or the fluidic system 4, in particular the receiving channel 4A extending below the separating device 6 or the filter.
  • the carrier 3 is preferably with a suitable structure, additional discharge channels 4D, which extend here, for example, like a ring, as indicated in Fig. 2, or the like. Mistake.
  • the inclusion of the (filtered) sample 2 in the fluidic system 4 or the receiving channel 4A and the reservoir 4B is preferably carried out (also) by capillary forces.
  • the filtered sample 2 then flows through the receiving channel 4A into the reservoir 4B.
  • the filtered sample 2 may also be used for other examination area, treatment area or the like. be forwarded.
  • the device 1 has a conveying device 7 for generating a negative pressure and / or overpressure or for conveying the sample 2.
  • the conveyor 7 serves, in particular, to effect, accelerate or assist the manipulation of the sample 2, in particular the filtration, and / or the subsequent output of the filtered sample 2, here from the reservoir 4B.
  • the conveyor 7 assists in the filtration and the inclusion of the (filtered) sample 2 in the fluidic system 4th acting capillary forces, so that in general the manipulation and in particular the filtration and recording of the sample 2 is supported.
  • the conveying device 7 or the negative pressure and / or overpressure generated by it can also significantly or exclusively effect the manipulation of the sample 2, in particular the filtration and / or recording of the sample 2.
  • the conveying device 7 comprises a deformation region 5A, which is formed in particular by the cover 5 or is integrally formed therewith.
  • the deformation region 5A is arranged above a recess or recess 7A, which is formed in the representation example, in particular in the carrier 3.
  • the conveying direction 7 or recess 7A is preferably connected via the connecting channel 4C to the fluidic system 4 or the reservoir 4B.
  • the deformation region 5A preferably spans the depression 7A in the illustration example at least substantially flat and / or flat.
  • the deformation region 5A is preferably deformable, in particular in order to generate a negative pressure and / or overpressure and / or to convey the sample 2.
  • the deformation region 5A is elastically deformable and tries to return after deformation by elastic restoring forces in the starting position.
  • the cover 5 is preferably made of an elastically deformable film or the like. educated. Furthermore, the cover 5 or the deformation region 5A can also be prestressed.
  • the deformation region 5 is deformed. This deformed state is indicated by dashed lines in FIG. 1A.
  • the pressing of the conveyor 7 or deformation of the deformation region 5A is preferably carried out manually, in particular with a thumb or other finger of a user, not shown, or in any other suitable manner.
  • the conveying device 7 preferably has a venting device 7B, which in the illustrated example has, in particular, a ventilation opening 7C, here in the bottom of the recess 7A or in the carrier 3, and an associated valve 7D for closing the venting opening 7C.
  • the bleeding The device 7B or ventilation opening 7C prevents an undesired overpressure from being generated in the recess 7A when the deforming region 5A is pressed or deformed into the recess 7A (the state deformed into the recess 7A is indicated by dashed lines in FIG. 1). since such an overpressure would counteract the desired flow direction of the sample 2 through the separating device 6 into the fluidic system 4, in particular into the reservoir 4B. Instead, displaced air may escape via the vent 7B or vent 7C.
  • the venting direction 7B or the valve 7D is closed in particular at the end of the deformation or depression of the deformation area 5A into the recess 7A, in particular by deforming the deformation area 5A or manually.
  • this is done in particular in that the deformation region 5 A comes to rest on the valve 7D, the valve 7D then being closed.
  • This is done here due to the pressure of the deformation of the deformation region 5A in particular by a user, not shown, for example by means of a thumb or other finger, or by means of another object, such as a tool or stamp or the like., Is exercised.
  • valve 7D closes automatically, for example, when a negative pressure in the recess 7A arises or air begins to flow from the outside through the vent opening 7C in the recess 7A. This happens, namely, when the deformation region 5A begins to return to its original, undeformed state after the deformation, in particular due to elastic recovery.
  • the deformed or depressed deformation region 5A (this state is indicated by dashed lines in FIG. 1) attempts to release it, in particular due to elastic recovery and / or under the action of another return or spring element (not shown), into its undeformed state Starting position to return.
  • a negative pressure is generated in the recess 7A, since the valve 7D or the venting device 7B is closed or remains or closes.
  • the fluidic system 4 which is fluidically connected to the recess 7A, here via the connection channel 4C, placed under negative pressure.
  • This negative pressure causes the sample 2 is sucked or is sucked into the fluidic system 4 and the reservoir 4B.
  • the sample 2 is sucked through the filter or the separating device 6 or supports the filtration and in particular substantially accelerated.
  • the volume of the conveyor 7 (volume of the recess 7A and / or the possible or expected deformation of the deformation region 5A) is preferably chosen such that it is substantially larger, in particular at least 50%, preferably at least 80%, than the volume of the sample 2, which is to be received by the separator 6, more specifically its filter, and by the fluidic system 4 and the reservoir 4B, respectively. It may also be provided for the design of the device 1, that the aforementioned volume of the conveyor 7 (volume of air, which is displaced from the recess 7 A when pressed or deformed) by at least a factor of 2, more preferably about a factor of 3 or more, larger than the volume of the sample 2 or the reservoir 4B to be received.
  • the sample 2 is taken up or sucked into the reservoir 4B.
  • the reservoir 4B for the (filtered) sample 2 and the conveyor 7 are preferably arranged on the carrier 3 separately from the trermet device 6 or in a second region 3B of the carrier 3.
  • the separating device 6 on the one hand and the reservoir 4B with the (filtered) sample 2 received therein and the conveying device 7 on the other hand or the two regions 3A and 3B (after filtration) are separable from one another.
  • the device 1 or the carrier 3 preferably has a predetermined breaking point 3C, the like, for example, by a corresponding material taper, a groove, a trench or the like. may be formed, as indicated in Fig. 1
  • the separation of the two regions 3A and 3B takes place in particular transversely to the receiving channel 4A or between the trermet device 6 and the reservoir 4B.
  • the predetermined breaking point 3C is accordingly preferably arranged in this area.
  • the separation of the two regions 3A and 3B takes place by bending the carrier 3 or the two regions 3A and 3B relative to one another, in the illustrated example particularly preferably around an axis transverse to the longitudinal extent of the carrier 3, transversely to the receiving channel 4A and / or in the plane of the plate of the carrier 3.
  • the separation of the two regions 3A and 3B can also be done, for example, by cutting, for example by means of a scissors or other cutting device, not shown, or in any other suitable manner.
  • the separation of the two areas 3A and 3B in the illustrated example particularly preferably causes the receiving channel 4A to be opened so that it can then be used as the dispensing channel for the sample 2 stored in the reservoir 4B or a dispensing opening for the sample Sample 2 or the device 1 or the carrier 3 forms.
  • the device 1 is preferably designed such that the conveying direction 7 or its recess 7A is automatically or automatically vented when separating the two areas 3A and 3B.
  • the separate carrier region 3B has the reservoir 4B with the (filtered) sample 2 received therein and the associated conveyor 7. This unit can now be stored with relatively little space and possibly even cooled, possibly even frozen.
  • the sample 2 can be removed or dispensed from the reservoir 4B, preferably by means of the conveyor 7.
  • the conveying device 7 is designed such that an overpressure can be generated in order to expel the sample 2 from the reservoir 4B, in particular via the receiving channel 4A.
  • the deformation region 5A can be deformed again, in particular pressed in.
  • the ventilation channels 7E must be closed. This can be achieved, for example, by the fact that the deformation region 5A engages with the manual depression on transition regions 7F of the ventilation channels 7E to the recess 7A and thereby closes them.
  • other constructive solutions are possible.
  • closing of ventilation and / or venting openings or channels may possibly also be dispensed with, since, for example, relatively rapid impressions lead to an optionally sufficiently high rise in the pressure in the recess 7A in order to be able to dispense the sample 2 from the reservoir 4B through the receiving channel 4A in a desired manner.
  • the removal of the filtered sample 2 from the reservoir 4B can alternatively also by piercing or piercing the cover 5 by means of a cannula, not shown o. The like. Or other opening of the fluidic system 4 or reservoir 4B and possibly sucking out in particular directly from the reservoir 4B done.
  • the connecting channel 4C is preferably selected to be long enough to prevent penetration of the sample 2 into the recess 7A.
  • the reservoir 4B is preferably designed in such a way that the sample 2 is held as compactly as possible in a region towards the receiving channel 4A in the reservoir 4B, in particular due to corresponding capillary forces, and / or that the sample 2 is only in the connecting channel when the reservoir 4B is completely filled 4C would occur.
  • the reservoir 4B is preferably in the form of a ramp, in particular for the aforementioned reasons, wherein the depth of the reservoir 4B preferably increases starting from the connection of the receiving channel 4A to the connection of the connecting channel 4C.
  • the carrier 3 is preferably formed flat and / or plate-like.
  • the carrier 3 is preferably at least substantially rigid.
  • the carrier 3 is preferably made of plastic, in particular injection-molded.
  • the channels, recesses, depressions and the like. for forming the fluidic system 4 are preferably formed on the flat side of the carrier 3, which is covered by the cover 5 (partially or completely).
  • the fluidic system 4 may also comprise the recess 7A, since it is connected thereto via the connection channel 4C.
  • the device 1 or the fluidic system 4 are, in particular, microfluidic arrangements.
  • Microfluidic in the sense of the present invention means that the individual volumes or total volumes are less than 5 ml, in particular less than 1 ml, particularly preferably less than 500 ⁇ .
  • the present invention particularly provides for the use of a vacuum or vacuum as a drive mechanism for a filtration process.
  • an overpressure can be used as the drive mechanism for the filtration process.
  • the present invention or device 1 provides, in particular, for impressing an elastic cover 5 or foil, preferably for generating an overpressure.
  • the preferably elastic return is utilized to generate a negative pressure.
  • the conveyor 7 preferably has a piston 7G or a piston-like member or the like. on.
  • the piston 7G is due to a relatively stiff surface area or the like. educated.
  • the piston 7G is formed by the deformation region 5A, here the recess 7A or another cavity of the Covered device 1 and the carrier 3.
  • the piston 7G or the deformation region 5A is movable into and / or out of the recess 7A and / or out of the recess 7A and / or beyond the flat side or outboard side of the carrier 3 to provide overpressure and / or To generate negative pressure or to promote the sample 2.
  • the conveyor 7 or the cover 5 which is particularly preferably formed plush-like, or their deformation region 5A preferably a fastening region 5B, which is particularly preferably tightly mounted on the support 3 and a flat side of the support 3 and
  • the recess 7A annularly or circumferentially surrounds, and / or a connecting portion 5C, which preferably connects the attachment portion 5B to the piston 7G and / or is elastically deformable or everting or at least one bent or everted deflection region 5D, on.
  • the turn-around connection portion 5C or deflection portion 5D allows easy movability of the piston 7G.
  • other constructive solutions are possible.
  • the deformation region 5A or connecting region 5C is preferably designed bellows-like or hose-like, in particular to allow easy deformation during the operation or use of the conveyor 7.
  • a possibly already preformed deflection takes place in the deflection region 5D by 180 ° in at least one section, in this case even in two sections of the deformation region 5A or connecting region 5C.
  • other constructive solutions are possible.
  • the conveyor 7 or the deformation region 5A preferably has an actuating element 7H, such as a tab, eyelet or the like. on. Particularly preferably, the actuation selement 7H is integrally formed.
  • the conveying device 7 is actuated in particular by the fact that the actuating element 7H or the piston 7G is moved into or out of the recess 7A. This in turn can be done manually or by means of a manipulating device of a drive, motor or the like. respectively.
  • a movement or main deformation takes place transversely, in particular at least in the sentlichen perpendicular to the main extension plane or flat side of the carrier 3.
  • a movement or main deformation takes place transversely, in particular at least in the sentlichen perpendicular to the main extension plane or flat side of the carrier 3.
  • other directions of movement and / or arrangements are possible.
  • the conveyor 7 or the cover 5 or the deformation region 5A is preferably integrally formed in the illustrated embodiment and / or made of plastic, in particular injection-molded. However, other constructive solutions are possible.
  • the deformation region 5A is preferably also formed in the second embodiment, that it assumes a preferred starting position, for example, the position shown in Fig. 3, in which the deformation region 5A and its piston 7G substantially in the recess 7 and in a is in the pressed-in position.
  • the deformation region 5A or piston 7G is then pulled out by means of the actuating element 7H and thereby preferably tensioned.
  • closed ventilation device 7B or closed valve 7D a negative pressure can be generated in the recess 7A which can act on the fluidic system 4 or the sample 3 to be manipulated via the connection channel 4C connected to the arrangement 7A.
  • the deformation region 5A or piston 7G can return to the starting position shown in FIG. 3, in particular due to elastic restoring forces, in order to generate an overpressure, as required, in particular with the venting direction 7B closed.
  • this return to the initial position does not have to be done by elastic return actuating forces, but in principle can also be supported by an active return movement, for example via the actuating element 7H, or exclusively.
  • the conveyor 7, if necessary, can also be designed or used only as a one-time operable device. In this case, for example, it is not necessary to make a provision. Rather, the deformation region 5A can be left in its deformation position after a single deformation. It should also be noted that the valve 7D indicated in the second embodiment in FIG. 3 or the venting device 7B shown there are merely optional. Depending on the construction or purpose, these can also be omitted.
  • the cover 5 or the deformation region 5A can also be formed separately from any other cover of the support 3 as required.
  • the cover 5 may, if necessary, also be designed in several parts, in particular with a separately formed deformation region 5A.
  • the cover 5 is formed at least substantially in the manner of a plug, at least in the region of the recess 7A or the deformation region 5A.
  • other training is possible.
  • the device 1 can also have a plurality of conveying devices 7. These can have separate deformation regions 5A, which in particular can be actuated independently of one another.
  • the various deformation regions 5A can optionally be formed by a common piece of material or by separate pieces of material or components.
  • the device 1 or conveying device 7 has a plurality of deformation regions 5A, these can, if necessary, also be actuated jointly and / or controlled by a common actuating device, offset in time from one another. Thus, according to the desired manner, an overpressure and / or negative pressure can be generated.
  • the different deformation regions 5A can then also exercise different functions in the device 1 or in the fluidic system 4, for example, via different channels.
  • the present invention or device 1 or conveying device 7 forms in particular a pump, which is particularly preferably manually operable.
  • the conveying device 7 or the deformation region 5A can, if necessary, both the non-deformed and the deformed position as each stable Assuming a situation, during the transition between a certain resistance must be overcome, for example in the manner of a so-called "crackpot frog".
  • the present invention and device 1 can be used for different samples 2, such as blood, urine, sputum, cell culture and fermentation media, waste water or the like, in particular for the retention or filtration of eukaryotic and prokaryotic cells, cell aggregates, protein - And lipidaggregaten or the like., Are used or used.
  • the present invention and device 1 allow filtration of microparticles, collectively so-called beads, which have a reagent bound to their surface, which have affinity for molecules from the sample 2 and by binding to concentrate target molecules located in the sample 2 (analyte, Biomarker or the like).
  • present invention and apparatus 1 can be used to remove molecules (e.g., proteins, nucleic acids) or particles (e.g., viruses) present in sample 2 and / or to purify, for example, by adsorptive binding.
  • molecules e.g., proteins, nucleic acids
  • particles e.g., viruses
  • the present invention and device 1 for example via indicators (dyes), the presence or absence of an existing or not present in the sample 2 target molecule and / or particles or the like. be used.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un dispositif comprenant un appareil de séparation pour la filtration d'un échantillon liquide et un appareil d'alimentation. Par l'appareil d'alimentation, une pression positive ou une pression négative peut être générée, pour accélérer ou favoriser la filtration.
EP11781496.2A 2010-11-10 2011-11-03 Dispositif de filtration du sang Withdrawn EP2637788A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11781496.2A EP2637788A1 (fr) 2010-11-10 2011-11-03 Dispositif de filtration du sang

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10014437 2010-11-10
PCT/EP2011/069350 WO2012062651A1 (fr) 2010-11-10 2011-11-03 Dispositif de filtration du sang
EP11781496.2A EP2637788A1 (fr) 2010-11-10 2011-11-03 Dispositif de filtration du sang

Publications (1)

Publication Number Publication Date
EP2637788A1 true EP2637788A1 (fr) 2013-09-18

Family

ID=43755258

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11781496.2A Withdrawn EP2637788A1 (fr) 2010-11-10 2011-11-03 Dispositif de filtration du sang

Country Status (4)

Country Link
US (1) US9116089B2 (fr)
EP (1) EP2637788A1 (fr)
JP (1) JP2013542445A (fr)
WO (1) WO2012062651A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013542445A (ja) 2010-11-10 2013-11-21 ベーリンガー インゲルハイム マイクロパーツ ゲゼルシャフト ミット ベシュレンクテル ハフツング 血液濾過器具
EP2695656A1 (fr) 2012-08-09 2014-02-12 F. Hoffmann-La Roche AG Procédé ainsi que dispositif de séparation destiné à séparer un filtrat d'un échantillon de liquide
EP2695655A1 (fr) 2012-08-09 2014-02-12 F. Hoffmann-La Roche AG Dispositif en plusieurs parties destiné à obtenir du plasma à partir de sang total
EP2695652A1 (fr) * 2012-08-09 2014-02-12 F. Hoffmann-La Roche AG Système de séparation du plasma depuis le sang total
JP5931709B2 (ja) * 2012-12-12 2016-06-08 株式会社ティー・ティー・エム 体液成分の検査器具
MX2017001368A (es) 2014-08-01 2017-05-11 Siemens Healthcare Diagnostics Inc Separacion de plasma asistido por vacio.
JP6382699B2 (ja) 2014-11-28 2018-08-29 株式会社東芝 マイクロ分析チップ
CN108795729B (zh) * 2018-09-28 2019-02-05 湖南乐准智芯生物科技有限公司 一种生物芯片及其微量液体进样结构
DE102021203636A1 (de) * 2021-04-13 2022-10-13 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Duo-Kartusche, mikrofluidische Analysevorrichtung, Verfahren zum Herstellen einer Duo-Kartusche und einer Analysevorrichtung und Verfahren zum Verwenden einer mikrofluidischen Analysevorrichtung

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991762B1 (en) * 1996-04-26 2006-01-31 Arkray, Inc. Device for analyzing a sample
JP3213566B2 (ja) * 1996-04-26 2001-10-02 アークレイ株式会社 検体分析用具およびそれを用いた検体分析方法並びに検体分析装置
JP3460143B2 (ja) * 1997-12-16 2003-10-27 アークレイ株式会社 試験液を吸引する引圧発生手段を備えた試験具
US6830934B1 (en) * 1999-06-15 2004-12-14 Lifescan, Inc. Microdroplet dispensing for a medical diagnostic device
DE19846466A1 (de) * 1998-10-08 2000-04-27 Ghs Gesundheits Service Ag Analyseverfahren zur simultanen Bestimmung von Parametern aus unterschiedlichen Medien
DE19933458B4 (de) 1999-07-15 2015-08-20 Eppendorf Ag Einrichtungen und Systeme zum Handhaben von Flüssigkeitsproben
US6627159B1 (en) * 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
CA2470350C (fr) * 2001-12-21 2010-11-09 3M Innovative Properties Company Remplissage centrifuge de dispositifs de traitement d'echantillons
DE10252223A1 (de) 2002-11-11 2004-05-27 Roche Diagnostics Gmbh Vorrichtung zur Separierung und Ausgabe von Plasma
DE102004027422A1 (de) 2004-06-04 2005-12-29 Boehringer Ingelheim Microparts Gmbh Vorrichtung zur Aufnahme von Blut und Abtrennung von Blutbestandteilen
BRPI0518969A2 (pt) * 2004-12-13 2008-12-16 Bayer Healthcare Llc sensor de teste independente
JP4721414B2 (ja) * 2005-08-15 2011-07-13 キヤノン株式会社 反応カートリッジ、反応装置および反応カートリッジの溶液の移動方法
JP5002266B2 (ja) * 2006-01-11 2012-08-15 キヤノン株式会社 体液採取装置
US8333712B2 (en) * 2006-01-11 2012-12-18 Canon Kabushiki Kaisha Body fluid sampling device
CN101500709A (zh) * 2006-05-01 2009-08-05 皇家飞利浦电子股份有限公司 用于加工、控制和/或检测流体样品并有减小的死体积的流体样品输送装置
US20080050830A1 (en) * 2006-05-10 2008-02-28 The Board Of Regents Of The University Of Texas System Detecting multiple types of leukocytes
US20080128341A1 (en) * 2006-12-04 2008-06-05 Electronics And Telecommunications Research Institute Micro filtration device for separating blood plasma and fabrication method therefor
JP5475692B2 (ja) 2008-02-27 2014-04-16 ベーリンガー インゲルハイム マイクロパーツ ゲゼルシャフト ミット ベシュレンクテル ハフツング 血漿の分離装置
JP2013542445A (ja) 2010-11-10 2013-11-21 ベーリンガー インゲルハイム マイクロパーツ ゲゼルシャフト ミット ベシュレンクテル ハフツング 血液濾過器具

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2012062651A1 *

Also Published As

Publication number Publication date
US9116089B2 (en) 2015-08-25
WO2012062651A1 (fr) 2012-05-18
JP2013542445A (ja) 2013-11-21
US20130341265A1 (en) 2013-12-26

Similar Documents

Publication Publication Date Title
WO2012062651A1 (fr) Dispositif de filtration du sang
EP2030684B1 (fr) Dispositif de préparation de substances pouvant être pipetées
EP1144095B1 (fr) Dispositif de concentration et/ou de purification de macromolecules en solution et procede de fabrication d'un tel dispositif
EP1880765B1 (fr) Système microliquide
EP2361599A1 (fr) Dispositif d'alimentation et d'extraction d'un liquide dans ou hors d'un récipient
WO2010037774A1 (fr) Dispositif microfluidique d'extraction et de réaction
WO2012127050A2 (fr) Dispositif et procédé pour filtrer du sang
WO2006128662A1 (fr) Microdistributeur et procede correspondant pour l'utiliser
DE10011235C2 (de) Ausstechvorrichtung zur Probenaufnahme und Verfahren zur Probenaufnahme
EP3230730B1 (fr) Boîtier de seringue à membranes intégrées pour le pipetage d'une matière biologique
DE102015102350B3 (de) Filtrationsvorrichtung für flüssige Proben
DE102014004851A1 (de) Vertikales funktionelles Reaktionsgefäß
DE102018111822B4 (de) Fluidisches System zur Aufnahme, Abgabe und Bewegung von Flüssigkeiten, Verfahren zur Verarbeitung von Fluiden in einem fluidischen System
DE102015205906A1 (de) Bevorratungseinheit, Verfahren zum Herstellen einer Bevorratungseinheit und Verfahren zum Freisetzen eines in einer Bevorratungseinheit gelagerten Fluids
EP2692440A1 (fr) Pièce d'insertion de flacon de réactif, flacon de réactif, procédé de centrifugation d'au moins un matériau et procédé de traitement sous pression d'au moins un matériau
DE102009001257A1 (de) Vorrichtung und Verfahren zur Handhabung von Flüssigkeiten
DE102013215565A1 (de) Vorrichtung zum Einbringen einer flüssigen Probe in ein mikrofluidisches System
DE10222478A1 (de) Verteilelement für Flüssigkeiten und Gase, Lab-on-a-Cip, Lab-on-a-Card
DE102013200363A1 (de) Mikrofluidisches Kanalsystem mit Blasenfängereinrichtung und Verfahren zum Entfernen von Gasblasen
EP2834006A1 (fr) Élément enceinte pour récipient à réactifs et son utilisation
DE102004038152B4 (de) Abdeckfolie für eine Mikrotiterplatte
DE102014207888A1 (de) Vorrichtung und Verfahren zur Durchführung von biochemischen Prozessen
DE102016123658B4 (de) Filtrationsvorrichtung und Verfahren zur Anreicherung von Targets und der nachfolgenden Freisetzung biogener Agenzien
WO2017203037A1 (fr) Récipient pour microéchantillons et procédé pour introduire un échantillon liquide dans un récipient pour microéchantillons
DE102013013003A1 (de) Vorrichtung und Verfahren zur Ablösung biologischen Materials von einer Oberfläche eines Trägers

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130610

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BLANKENSTEIN, GERT

Owner name: GROLLA, MARC

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20180602