EP2593406A1 - Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture - Google Patents

Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture

Info

Publication number
EP2593406A1
EP2593406A1 EP10745684.0A EP10745684A EP2593406A1 EP 2593406 A1 EP2593406 A1 EP 2593406A1 EP 10745684 A EP10745684 A EP 10745684A EP 2593406 A1 EP2593406 A1 EP 2593406A1
Authority
EP
European Patent Office
Prior art keywords
substrate
environment
natural
extracts
bioremediation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10745684.0A
Other languages
German (de)
French (fr)
Inventor
Lazaro Sebastiao Roberto
Luiz Antonio Alves Pereira
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ellingsen Hans Falnes
Walvag Kolfinn Roger
Original Assignee
Ellingsen Hans Falnes
Walvag Kolfinn Roger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ellingsen Hans Falnes, Walvag Kolfinn Roger filed Critical Ellingsen Hans Falnes
Publication of EP2593406A1 publication Critical patent/EP2593406A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
    • C02F1/683—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water by addition of complex-forming compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/32—Hydrocarbons, e.g. oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/40—Valorisation of by-products of wastewater, sewage or sludge processing

Definitions

  • the present request for an invention patent refers to an innovative technology developed from the use of enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where a synthesis reaction creating expressive conditions for bioremediation of carbon molecules takes place.
  • the density is lower than water (even when associated to the oils) and chemically apolar (suffering total rejection by the aquatic environment) granting permanent floatability, impeding the reintroduction of the polluting element into the aquatic environment, and when on land, it impedes the mechanical action of the oils in the gas exchange processes between free 0 2 and the epicyclic environment when the polluting element is still in a contaminant stage.
  • the production technology of the present invention has three distinct phases, which are:

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Soil Sciences (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The present development brings a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces. It is a process of using enzymes for bioremediation in a substrate, which due to its basic substances' characteristics, presents an inexpressive toxicity, high floatability degree and the ability to absorb contaminant in a ratio weight/weight in the range of 2.000% to 2.500%, and in the ratio volume/volume in the range of 100% to 200%. In the present process we present a technological innovation for manipulation of natural-origin enzymatic elements, using them in a specific way and for a specific purpose, which due to these characteristics, when in contact with liquid carbon substances provide a considerable reduction of the biodegradability time and the transformation of the bioremediator/polluent mix when collected in fully organic compost.

Description

"NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE
APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT
CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE".
SPECIFICATION
The present request for an invention patent refers to an innovative technology developed from the use of enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where a synthesis reaction creating expressive conditions for bioremediation of carbon molecules takes place.
The necessity of the present development becomes evident when we face the reality of a permanent environmental threat: pollution by liquid substances containing carbon molecules, occasioned by human failure or break-down of systems or equipment.
When there is an accident, the methodology presently applied for the absorption of liquid carbon substances from the environment is using absorbing materials. However, due to its non-degradability, these materials have the inconvenience of substantially increasing the residue volume, sometimes causing worse harm to the environment. In some cases (like in the water), due to higher density and/or affinity with the aquatic environment, it makes the oil sink. In other cases, the lixiviation processes (the oil initially absorbed comes loose), make the pollutant return to the environment, and on land, it impedes the mechanic action of the oils in the gas exchange processes between free O2 and the epicyclic substrate.
The utilization of natural-origin polymeric enzymes as substrate in an environment consisting of extracts of oleaginous vegetable sources, added to a mixture of isomer, has considerable advantages in relation to the above mentioned products for the removal of oily substances, because they are able to quickly transform the substances into a totally organic compound by action of the mentioned enzymes in this specific substrate (which, due to its qualities, foment an electrovalense action with the carbon molecules, adding it to the environment, which immediately puts an end to the aggression against the ecosystem). The density is lower than water (even when associated to the oils) and chemically apolar (suffering total rejection by the aquatic environment) granting permanent floatability, impeding the reintroduction of the polluting element into the aquatic environment, and when on land, it impedes the mechanical action of the oils in the gas exchange processes between free 02 and the epicyclic environment when the polluting element is still in a contaminant stage.
The production technology of the present invention has three distinct phases, which are:
1) - Mixing process of substrate - This phase proceeds from the mixture of extract of oleaginous vegetable sources with a mix of isomers forcing a binding and expansion reaction and the appearance of a polymeric substrate;
2) - Addition of predefined proportions of natural-origin polymeric biomolecules - Specific doses of: Oxdoreductases 12,5g, Hydroxylases 10g, Ligases 8,5g e Isomerases 10g on a culture base per m3;
3) Final processing - Structural physical modification of the product resulting from the enzymatic addition to the polymeric substrate.
The object of the present request for the Invention Patent, called "NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE" is an innovative enzymatic technology
introduced in a substrate of oleaginous vegetable extracts added to a mixture of isomers for the bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces, with an efficiency that is superior to existing technologies and products. Because the substrate is granulated, thus causing the addition to its environment of liquid oily contaminating substances in a ratio of weight/weight in the range of 2.000% to 2.500% and in the ratio volume/volume in the range of 100% to 200%, which, due to the substances' basic characteristics has an inexpressive toxicity degree and a high floatability degree, we have in this process come up with a technologic innovation which is a methodology of
manipulating the enzymatic elements from natural origin, using them in a specific way and for a specific purpose.

Claims

The present request for an invention patent refers to a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces.
Claim 1 , consists of a process for mixing the substrate where the extracts of oleaginous vegetable sources are blended into a mixture of isomers, causing a reaction of binding and expansion and the emergence of a polymeric substrate;
Claim 2, consists of addition in predefined proportions of natural origin polymeric biomolecules in specific doses of: Oxdoreductases 125g, Hydroxylases 100g, Ligases 80, 5g e Isomerases 100g on a culture base per m3;
Claim 3, consists of the final processing of the physical structural modification of the product which resulted from the enzymatic addition to the polymeric substrate.
EP10745684.0A 2010-07-14 2010-07-14 Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture Withdrawn EP2593406A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/NO2010/000284 WO2012008841A1 (en) 2010-07-14 2010-07-14 Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture

Publications (1)

Publication Number Publication Date
EP2593406A1 true EP2593406A1 (en) 2013-05-22

Family

ID=43778166

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10745684.0A Withdrawn EP2593406A1 (en) 2010-07-14 2010-07-14 Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture

Country Status (3)

Country Link
EP (1) EP2593406A1 (en)
BR (1) BR112013000948B1 (en)
WO (1) WO2012008841A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018170561A1 (en) * 2017-03-22 2018-09-27 Kolfinn Roger Walvag Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2278348B (en) * 1993-05-25 1997-03-05 Int Bioremediation Serv Ltd Method of reducing contamination and composition for use in the method
US5656490A (en) * 1993-07-23 1997-08-12 Wyatt; Caryl Heintz Enzyme compositions and methods for biodegradation separation of natural fibers and adsorbed petroleum products
JPH10292358A (en) * 1997-04-16 1998-11-04 Ishikawa Giken Rubber Kk Recovering method of oil
BRPI0703623A2 (en) * 2007-10-03 2009-05-19 Luiz Antonio Alves Pereira improved system and manufacturing process of bioremediation product of liquid substances containing carbon molecules in their structure

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2012008841A1 *

Also Published As

Publication number Publication date
BR112013000948A2 (en) 2020-06-09
BR112013000948B1 (en) 2021-05-25
WO2012008841A1 (en) 2012-01-19

Similar Documents

Publication Publication Date Title
AlAmeri et al. Sorption and removal of crude oil spills from seawater using peat-derived biochar: An optimization study
Baccar et al. Modeling of adsorption isotherms and kinetics of a tannery dye onto an activated carbon prepared from an agricultural by-product
Xu et al. Effect of bamboo biochar on pentachlorophenol leachability and bioavailability in agricultural soil
Santi et al. Reduction of organic pollutants in olive mill wastewater by using different mineral substrates as adsorbents
Gurav et al. Adsorptive removal of synthetic plastic components bisphenol-A and solvent black-3 dye from single and binary solutions using pristine pinecone biochar
Zhou et al. Effect of Tween 80 and β-cyclodextrin on degradation of decabromodiphenyl ether (BDE-209) by White Rot Fungi
Dahri et al. Removal of methyl violet 2B from aqueous solution using Casuarina equisetifolia needle
Sayyad Amin et al. Natural sorbent for oil spill cleanup from water surface: environmental implication
Chidambaram Rice husk as a low cost nanosorbent for 2, 4-dichlorophenoxyacetic acid removal from aqueous solutions
CN102500340B (en) Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle
Rizvi et al. A holistic approach for melanoidin removal via Fe-impregnated activated carbon prepared from Mangifera indica leaves biomass
Pigatto et al. Chitin as biosorbent for phenol removal from aqueous solution: Equilibrium, kinetic and thermodynamic studies
Cardoso et al. Remediation of petroleum contaminated saline water using value-added adsorbents derived from waste coconut fibres
WO2011038459A8 (en) Remediation composition comprising alum sludge
Ranc et al. Comparison of the effectiveness of soil heating prior or during in situ chemical oxidation (ISCO) of aged PAH-contaminated soils
Ma et al. Water-promoted catalytic hydrodechlorination of transformer oil-contained PCBs in liquid system under mild conditions
Cao et al. Removal of Pb, Zn, and Cd from contaminated soil by new washing agent from plant material
Ye et al. Enhanced soil washing process for the remediation of PBDEs/Pb/Cd-contaminated electronic waste site with carboxymethyl chitosan in a sunflower oil–water solvent system and microbial augmentation
Sun et al. Biosorption and biodegradation of BDE-47 by Pseudomonas stutzier
Devi et al. Effect of Co‐culture and nutrients supplementation on bioremediation of crude petroleum sludge
Zahed et al. Response surface analysis to improve dispersed crude oil biodegradation
Carvalho et al. Effect of alkaline treatment on the fruit peel of Pachira aquatic Aubl.: Physico-chemical evaluation and characterization
Chang et al. Fabrication of novel rhamnolipid-oxygen-releasing beads for bioremediation of groundwater containing high concentrations of BTEX
Chen et al. Selective adsorption and efficient degradation of petroleum hydrocarbons by a hydrophobic/lipophilic biomass porous foam loaded with microbials
Kończak et al. Chemical properties of soil determine the persistence and bioavailability of polycyclic aromatic hydrocarbons in sewage sludge-or sewage sludge/biomass-derived biochar-amended soils

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130129

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20160202