EP2573110A2 - Peptide vaccines with Seq Id No: 174, 178, 186 or 194 for cancers expressing tumor-associated antigens - Google Patents

Peptide vaccines with Seq Id No: 174, 178, 186 or 194 for cancers expressing tumor-associated antigens Download PDF

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EP2573110A2
EP2573110A2 EP12175487A EP12175487A EP2573110A2 EP 2573110 A2 EP2573110 A2 EP 2573110A2 EP 12175487 A EP12175487 A EP 12175487A EP 12175487 A EP12175487 A EP 12175487A EP 2573110 A2 EP2573110 A2 EP 2573110A2
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seq
ctl
hla
cancer
peptide
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EP2573110A3 (en
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Takuya Tsunoda
Ryuji Ohsawa
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Oncotherapy Science Inc
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Oncotherapy Science Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08—Peptides having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K39/0011—Cancer antigens
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    • A61K39/0011—Cancer antigens
    • A61K39/001102—Receptors, cell surface antigens or cell surface determinants
    • A61K39/001122—Ephrin Receptors [Eph]
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    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464422—Ephrin Receptors [Eph]
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/46443—Growth factors
    • A61K39/464434—Transforming growth factor [TGF]
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    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464454—Enzymes
    • A61K39/464462—Kinases, e.g. Raf or Src
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464466—Adhesion molecules, e.g. NRCAM, EpCAM or cadherins
    • A—HUMAN NECESSITIES
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    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4748—Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
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    • C12N2510/00—Genetically modified cells

Definitions

  • the present invention relates to the field of biological science, more specifically to the field of cancer therapy.
  • the present invention relates to novel immunogenic peptides that serve as extremely effective as cancer vaccines, and drugs for treating and preventing tumors containing such peptides.
  • CTLs cytotoxic T lymphocytes
  • TAAs tumor-associated antigens
  • TAAs discovered to date include MAGE ( van der Bruggen P et al., (1991) Science 254: 1643-7 .), gp100 ( Kawakami Y et al., (1994) J Exp Med 180: 347-52 .), SART ( Shichijo S et al., (1998) J Exp Med 187:277-88 .), and NY-ESO-1 ( Chen Y.T. et al., (1997) Proc. Natl. Acd. Sci. USA, 94: 1914-8 .).
  • certain gene products demonstrated to be somewhat specifically over-expressed in tumor cells have been shown to be recognized as targets for inducing cellular immune responses.
  • Such gene products include p53 ( Umano Y et al., (2001) Br J Cancer, 84:1052-7 .), HER2/neu ( Tanaka H et al., (2001) Br J Cancer, 84: 94-9 .), CEA ( Nukaya I et al., (1999) Int. J. Cancer 80, 92-7 .) and the like.
  • TAAs that are abundantly expressed in cancer cells, and whose expression is restricted to cancer cells, would be promising candidates as immunotherapeutic targets.
  • HLA-A24 and HLA-A020 are common HLA alleles in the Japanese and Caucasian populations ( Date Y et al., (1996) Tissue Antigens 47: 93-101 .; Kondo A et al., (1995) J Immunol 155: 4307-12 .; Kubo RT et al., (1994) J Immunol 152: 3913-24 .; Imanishi et al., Proceeding of the eleventh International Histocompatibility Workshop and Conference Oxford University Press, Oxford, 1065 (1992 ); Williams F et al., (1997) Tissue Antigen 49: 129-33 .).
  • antigenic peptides of cancers presented by these HLA alleles may find particular utility in the treatment of cancers among Japanese and Caucasian patients.
  • the induction of low-affinity CTL in vitro usually results from exposure to high concentrations of peptides, generating a high level of specific peptide/MHC complexes on antigen-presenting cells (APCs), which will effectively activate these CTL ( Alexander-Miller et al., (1996) Proc Natl Acad Sci USA 93: 4102-7 .).
  • HLA class 1-binding peptide sequence can be expected using algorithms ( Jounal of Immunological Methods, (1995), Vol.185, pp.181-190 , J. Immunol., (1994), Vol.152, pp.163-175 , protein science, (2000), Vol.9, pp.1838-1846 ).
  • the expected epitope peputide can be cut to the size and expressed on the target cell surface with HLA molecule and recognized by CTL.
  • CDH3 GenBank Accession No. NM_001793; SEQ ID Nos.1, 2), EPHA4 (GenBank Accession No. L36645; SEQ ID Nos.3, 4), ECT2 (GenBank Accession No. AY376439; SEQ ID Nos.5, 6), HIG2 (GenBank Accession No. NM_013332; SEQ ID Nos.7, 8) INHBB, (GenBank Accession No. NM_002193; SEQ ID Nos.9, 10), KIF20A (GenBank Accession No. NM_005733; SEQ ID Nos.11, 12), KNTC2 (GenBank Accession No.
  • genes identified as over-expressed in various cancers include, but are not limited to, CDH3, EPHA4, ECT2 HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10.
  • CDH33 has been previously identified as over-expressed in bladder cancer, cervical cancer, cholangincellular carcinoma, colorectal cancer, endometriosis, gastric cancer, diffuse-type gastric cancer, non-small cell lung cancer (NSCLC), pancreatic cancer, soft tissue tumor and testicular tumor.
  • EPHA4 has been identified in bladder cancer, cervical cancer, cholangincellular carcinoma, endometriosis, diffuse-type gastric cancer, ovarian cancer, pancreatic cancer, prostate cancer and soft tissue tumor.
  • ECT2 has been identified in bladder cancer, breast cancer, cervical cancer, cholangineellular carcinoma, chronic myeloid leukemia (CML), colorectal cancer, esophageal cancer, NSCLC, lymphoma, prostate cancer, renal carcinoma and small cell lung cancer (SCLC).
  • HIG2 has been identified in renal carcinoma and SCLC.
  • INHBB has been identified in cholangincellular carcinoma, esophageal cancer, NSCLC, renal carcinoma, SCLC and soft tissue tumor.
  • KIF20A has been identified in bladder cancer, breast cancer, cholangincellular carcinoma, esophageal cancer, NSCLC, pancreatic cancer, prostate cancer, renal carcinoma and SCLC.
  • KNTC2 has been identified in bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, esophageal cancer, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC and soft tissue tumor.
  • TTK has been identified in bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, esophageal cancer, liver cancer, NSCLC, lymphoma, osteosarcoma, prostate cancer, SCLC and soft tissue tumor.
  • URLC10 has been identified in bladder cancer, cervical cancer, cholangincellular carcinoma, esophageal cancer, gastric cancer, NSCLC, osteosarcoma, pancreatic cancer and SCLC.
  • the present invention is based in part on the discovery of the applicable targets of immunotherapy. Because TAAs have often no immunogenicity, the discovery of appropriate targets is of extreme importance.
  • CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 have been identified as up-regulated in various cancers. More particularly, these genes were identified using gene expression profiling with a genome-wide cDNA microarray. As discussed above, expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 has been shown to be specifically up-regulated in various tumor cells, from pancreatic cancer cells to renal cell carcinomas.
  • CDH3 expression is validly elevated in 26 out of 34 bladder cancer, 17 out of 19 cervical cancer, all of 19 cholangincellular carcinoma, 30 out of 34 colorectal cancer, 20 out of 21 endometriosis, 13 out of 20 gastric cancer, 7 out of 8 diffuse-type gastric cancer, 36 out of 37 NSCLC, all of 16 pancreatic cancer, all of 21 soft tissue tumor and all of 10 testicular tumor.
  • the present invention is based, at least in part, on the identification of specific epitope peptides of the gene products of these genes (CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10) which possess the ability to induce cytotoxic T lymphocytes (CTLs) specific to the corresponding molecules.
  • CTLs cytotoxic T lymphocytes
  • PBMC Peripheral Blood Mononuclear Cells
  • the present invention provides methods for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10, e.g. cancer.
  • Such methods involve the step of administering to a subject in need thereof a CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 polypeptides of the invention.
  • Administration of such peptide(s) results in the induction of anti-tumor immunity is induced by the administration of these polypeptides.
  • the present invention provides methods for inducing anti-tumor immunity in a subject, such methods involving the step of administering to the subject the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 polypeptides, as well as pharmaceutical compositions for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer, that include the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 polypeptides.
  • cancers include, but are not limited to., bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the present invention further provides methods for preventing post-surgery recurrence of the disease mentioned above.
  • the specific aims and objectives recited above it will be understood by those skilled in the art that one or more aspects of this invention can meet certain objectives, while one or more other aspects can meet certain other objectives.
  • Each objective may not apply equally, in all its respects, to every aspect of this invention.
  • the objects herein can be viewed in the alternative with respect to any one aspect of this invention.
  • the present invention is based in part on the discovery of applicable targets of immunotherapy. Identification of new TAAs, particularly those that induce potent and specific anti-tumor immune responses, warrants further development of the clinical application of the peptide vaccination strategy in various types of cancer ( Boon T et al., (1996) J Exp Med 183: 725-9 .; van der Bruggen P et al., (1991) Science 254: 1643-7 .; Brichard V et al., (1993) J Exp Med 178: 489-95 .; Kawakami Y et al., (1994) J Exp Med 180: 347-52 .; Shichijo S et al., (1998) J Exp Med 187:277-88 .; Chen YT et al., (1997) Proc.Natl.Acd.
  • peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 are shown to be TAA epitopes restricted by HLA-A24 and HLA-A2, an HLA allele commonly found in the Japanese and Caucasian populations. Specifically, using their binding affinities to HLA-A24 or HLA-A2, candidates of HLA-A24 or HLA-A2 binding peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 were identified. After the in vitro stimulation of T-cells by dendritic cells (DCs) loaded with these peptides, CTLs were successfully established using the following peptides.
  • DCs dendritic cells
  • peptides are epitope peptides of each TAA restricted by HLA-A24 or HLA-A2. Since these antigens are over-expressed in most cancers and are associated with tumor cell proliferation, they find utility as immunotherapeutic targets against cancers.
  • Exemplary cancers include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the present invention further provides methods of treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers in a subject, such methods including the steps of administering to the subject an immunogenic peptide of less than about 40 amino acids, often less than about 20 amino acids, usually less than about 15 amino acids and having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288.
  • the immunogenic peptide may have an amino acid sequence as set forth in SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 in which 1, 2, or several (e.g., up to 5) amino acids are substituted, deleted or added, provided the resulting variant peptide retains the immunogenic activity (i.e., the ability to induce CTLs specific to cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers).
  • the number of residues to be substituted, deleted, or added is generally 5 amino acids or less, preferably 4 amino acids or less, more preferably 3 amino acids or less, even more preferably one or two amino acids.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. Furthermore the present invention provides methods for preventing post-surgery recurrence of these diseases mentioned above.
  • Variant peptides i.e., peptides having an amino acid sequence modified by substituting, deleting, or adding one, two or several amino acid residues to an original amino acid sequence
  • Variant peptides are known to retain the original biological activity ( Mark DF et al., (1984) Proc Natl Acad Sci USA 81: 5662-6 .; Zoller MJ and Smith M, (1982) Nucleic Acids Res 10:6487-500 .; Dalbadie-McFarland G et al., (1982) Proc Natl Acad Sci USA 79: 6409-13 .).
  • it is preferable that the amino acid modification results in conservation of the properties of the original amino acid side-chain (a process known as conservative amino acid substitution).
  • amino acid side chains examples include hydrophobic amino acids (A, I, L, M, F, P, W, Y, V), hydrophilic amino acids (R, D, N, C, E, Q, G, H, K, S, T), and side chains having the following functional groups or characteristics in common: an aliphatic side-chain (G, A, V, L, I, P); a hydroxyl group containing side-chain (S, T, Y); a sulfur atom containing side-chain (C, M); a carboxylic acid and amide containing side-chain (D, N, E, Q); a base containing side-chain (R, K, H); and an aromatic containing side-chain (H, F, Y, W).
  • A, I, L, M, F, P, W, Y, V hydrophilic amino acids
  • R, D, N, C, E, Q amino acids
  • G, A, V, L, I, P a hydroxyl group containing side-chain
  • the immunogenic peptide is a nonapeptide (9-mer) or a decapeptide (10-mer).
  • the present invention further provides a method of inducing anti-tumor immunity for a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g.
  • cancers in a subject, such a method including the steps of administering to the subject an immunogenic peptide of the present invention, namely one having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288, or a variant thereof (i.e., including 1, 2, or several (e.g., up to 5) amino acid substitutions, deletions, or additions) to the subject in need thereof.
  • an immunogenic peptide of the present invention namely one having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the subject is preferably a mammal.
  • exemplary mammals include, but are not limited to, e.g., a human, non-human primate, mouse, rat, dog, cat, horse, or cow.
  • the peptide can be administered to a subject via an in vivo or ex vivo protocol.
  • the present invention also provides use of nonapeptide or decapeptide selected from peptides having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 (and variants thereof) for manufacturing an immunogenic composition for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, K11TTC2, TTK and/or URLC10, e.g.
  • cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • HLA antigens the data presented here demonstrate that the uses of A-24 type or A-2 type antigens (which are said to be highly expressed among the Japanese) are favorable for obtaining effective results.
  • the uses of subtypes such as A-2402 and A-0201 are even more preferable.
  • the type of HLA antigen of the patient requiring treatment is investigated in advance, which, in turn, enables the selection of appropriate peptides having high levels of binding affinity to the patient antigen, or having cytotoxic T cell (CTL) inducibility by antigen presentation.
  • CTL cytotoxic T cell
  • substitution, deletion, or addition of 1, 2, or several (e.g., up to 5) amino acids may be performed based on the amino acid sequence of the naturally occurring CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 partial peptide.
  • the term "several” means refers to 5 or less, more preferably 3 or less.
  • peptides whose C-terminal amino acid is substituted with phenylalanine, leucine, isoleucine, tryptophan, or methionine may also be used favorably.
  • peptides possessing high HLA-A2 binding affinity in which the second amino acid from the N terminus substituted with leucine or methionine, and peptides whose C-terminal amino acid is substituted with valine or leucine may be used favorably. The substitution is performed not only at the terminus amino acids but also at the position of potential TCR recognition of peptides.
  • amino acid substitutions in a peptide can be equal to or better than the original, for example CAP1, p53 (264-272) , Her-2/neu (369-377) or gp100 (209-217) ( Zaremba et al. Cancer Res. 57, 4570-4577, 1997 , T. K. Hoffmann et al. J Immunol. (2002) Feb 1;168(3):1338-47 ., S. O. Dionne et al. Cancer Immunol immunother. (2003) 52: 199-206 and S. O. Dionne et al. Cancer Immunology, Immunotherapy (2004) 53, 307-314 ). Furthermore, 1 to 2 amino acids may be added to the N terminus and/or C terminus of the peptide.
  • the peptide sequence is identical to a portion of the amino acid sequence of an endogenous or exogenous protein having a different function, side effects such as autoimmune disorders or allergic symptoms against specific substances may be induced. Therefore, it is preferable to avoid the situation wherein the immunogenic sequence matches the amino acid sequence of a known protein. This situation may be avoided by performing a homology search using available databases. If homology searches confirm that peptides in which 1, 2 or several different amino acids do not exist in nature, then the danger that modifications of the above-mentioned amino acid sequence that, for example, increase the binding affinity with HLA antigens, and/or increase the CTL inducibility can be avoided.
  • CTL inducibility may be routinely confirmed by inducing antigen-presenting cells carrying human MHC antigens (for example, B-lymphocytes, macrophages, and dendritic cells), or more specifically dendritic cells derived from human peripheral blood mononuclear leukocytes, and, after stimulation with the peptide of interest, mixing with CD8-positive cells and measuring the cytotoxic activity against the target cells.
  • human MHC antigens for example, B-lymphocytes, macrophages, and dendritic cells
  • transgenic animals produced to express a human HLA antigen may be used.
  • the target cells can be radiolabeled with 51 Cr and such, and cytotoxic activity can be calculated from radioactivity released from the target cells.
  • it can be examined by measuring IFN-gamma produced and released by CTL in the presence of antigen-presenting cells that carry immobilized peptides, and visualizing the inhibition zone on the media using anti-IFN-gamma monoclonal antibodies.
  • the present invention provides peptides having cytotoxic T cell inducibility, namely those having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof (i.e., those in which 1, 2, or several amino acids are substituted, deleted, or added).
  • amino acid sequences composed of 9 or 10 amino acids indicated in SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof do not match an amino acid sequence associated with another endogenous protein.
  • amino acid substitution to leucine or methionine at the second amino acid from the N terminus amino acid substitution to valine or leucine at the C-terminal amino acid, and amino acid addition of 1 to 2 amino acids at the N terminus and/or C terminus are examples of preferred variants.
  • immunologically active fragments of the peptides may also be used in the methods of the invention. Methods for determining active fragments are well known in the art. CTL clones obtained by stimulation by these modified peptides can recognize the original peptides and cause damage for cells expressing the original peptides.
  • Peptides of the present invention can be prepared using well known techniques.
  • the peptides can be prepared synthetically, using either recombinant DNA technology or chemical synthesis.
  • Peptides of the present invention may be synthesized individually or as longer polypeptides composed of two or more peptides.
  • the peptides of the present invention are preferably isolated, i.e., substantially free of other naturally occurring host cell proteins and fragments thereof.
  • the peptides of the present invention may contain modifications, such as glycosylation, side chain oxidation, or phosphorylation; so long as the modifications do not destroy the biological activity of the peptides as described herein, namely the ability to binding to an HLA antigen and induce CTL.
  • modifications include incorporation of D-amino acids or other amino acid mimetics that can be used, for example, to increase the serum half life of the peptides.
  • this invention may contain a method of screening for a peptide which 1, 2, or several amino acids are substituted, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223, said method comprising the steps of:
  • the present invention provides a method of identifying for a peptide having an ability to induce CTL against cells expressing at leaset one tumor-associated antigen, wherein the tumor-associated antigen is antigen selected from the group consisting of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10, said method comprising the steps of:
  • the amino acid is substituted for a different amino acid in which the properties of the amino acid side-chain are conserved (a process known as conservative amino acid substitution).
  • properties of amino acid side chains are hydrophobic amino acids (A, I, L, M, F, P, W, Y, V), hydrophilic amino acids (R, D, N, C, E, Q, G, H, K, S, T), and side chains having the following functional groups or characteristics in common: an aliphatic side-chain (G, A, V, L, I, P); a hydroxyl group containing side-chain (S, T, Y); a sulfur atom containing side-chain (C, M); a carboxylic acid and amide containing side-chain (D, N, E, Q); a base containing side-chain (R, K, H); and an aromatic containing side-chain (H, F, Y, W).
  • the parenthetic letters indicate the one-letter codes of amino acids. In the present invention, substantial significant homology is, for example, amino
  • the peptides of this invention can be prepared as a combination, which includes two or more of peptides of the invention, for use as a vaccine for a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, such a vaccine inducing CTL in vivo.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the peptides may be in a cocktail or may be conjugated to each other using standard techniques.
  • the peptides can be expressed as a single polypeptide sequence.
  • the peptides in the combination may be the same or different.
  • the peptides are presented at a high density on the HLA antigens of antigen-presenting cells, which, in turn, induces CTLs that specifically react toward the complex formed between the displayed peptide and the HLA antigen.
  • antigen-presenting cells having immobilized the peptides of this invention on their cell surface obtained by removing dendritic cells from the subjects, may be stimulated by the peptides of this invention. Re-administration of these cells to the respective subjects induces CTL, and, as a result, aggressiveness towards the target cells can be increased.
  • the present invention provides drugs for treating and/or preventing proliferation, metastasis, and such of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, which include one or more of peptides of the present invention, or a polynucleotide encoding the peptides.
  • the peptides or polynucleotides of the present invention find particular utility in the treatment of a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the peptides of this invention can be administered to a subject directly, as a pharmaceutical composition that has been formulated by conventional formulation methods.
  • carriers, excipients, and such that are ordinarily used for drugs can be included as appropriate, without particular limitations.
  • the immunogenic compositions of this invention may be used for treatment and prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the immunogenic compositions for treatment and/or prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 e.g. cancers, which include as the active ingredient one or more peptides of the present invention, can further include an adjuvant so that cellular immunity will be established effectively. Alternatively, they may be administered with other active ingredients, such as anti-cancer agents.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • Suitable formulations include granules. Suitable adjuvants are described in the literature ( Johnson AG. (1994) Clin. Microbiol. Rev., 7:277-89 .).
  • Exemplary adjuvants include, but are not limited to, aluminum phosphate, aluminum hydroxide, and alum. Furthermore, liposome formulations, granular formulations in which the drug is bound to few-mc m diameter beads, and formulations in which a lipid is bound to the peptide may be conveniently used.
  • the method of administration may be oral, intradermal, subcutaneous, intravenous injection, or such, and may include systemic administration or local administration to the vicinity of the targeted tumor.
  • the dose of the peptide(s) of this invention can be adjusted appropriately according to the disease to be treated, age of the patient, weight, method of administration, and such. Though the dosage is ordinarily 0.001 mg to 1000 mg, preferably 0.01 mg to 100 mg, more preferably 0.1 mg to 10 mg, preferably administered once in a few days to few months, one skilled in the art can readily select the appropriate dose and method of administration, as, the selection and optimization of these parameters is well within routine skill.
  • the present invention further provides intracellular vesicles called exosomes, which present complexes formed between the peptides of this invention and HLA antigens on their surface.
  • Exosomes can be prepared, for example, by using the methods described in detail in Published Japanese Translation of International Publication Nos. Hei 11-510507 and 2000-512161 , and are preferably prepared using antigen-presenting cells obtained from subjects who are targets of treatment and/or prevention.
  • the exosomes of this invention can be inoculated as cancer vaccines, similarly to the peptides of this invention.
  • HLA-A24 or HLA-A2 particularly HLA-A2402 or HLA-A0201, is often appropriate.
  • the vaccine compositions of the present invention include a component which primes cytotoxic T lymphocytes.
  • Lipids have been identified as agents capable of priming CTL in vivo against viral antigens.
  • palmitic acid residues can be attached to the epsilon-and alpha-amino groups of a lysine residue and then linked to an immunogenic peptide of the invention.
  • the lipidated peptide can then be administered either directly, in a micelle or particle, incorporated into a liposome, or emulsified in an adjuvant.
  • a lipid priming of CTL responses E.
  • coli lipoproteins such as tripalmitoyl-S-glycerylcysteinlyseryl- serine (P3CSS)
  • P3CSS tripalmitoyl-S-glycerylcysteinlyseryl- serine
  • the immunogenic compositions of the present invention may also include nucleic acids encoding one or more of the immunogenic peptides disclosed here. See, e.g., Wolff JA et al., (1990) Science 247:1465-8 ; U.S. Patent Nos. 5,580,859 ; 5,589,466 ; 5,804,566 ; 5,739,118 ; 5,736,524 ; 5,679,647 ; and WO 98/04720 .
  • DNA-based delivery technologies include "naked DNA", facilitated (bupivicaine, polymers, peptide-mediated) delivery, cationic lipid complexes, and particle-mediated (“gene gun”) or pressure-mediated delivery (see, e.g., U.S. Patent No. 5,922,687 ).
  • the immunogenic peptides of the invention can also be expressed by viral or bacterial vectors.
  • suitable expression vectors include attenuated viral hosts, such as vaccinia or fowlpox. This approach involves the use of vaccinia virus, e.g., as a vector to express nucleotide sequences that encode the peptide. Upon introduction into a host, the recombinant vaccinia virus expresses the immunogenic peptide, and thereby elicits an immune response.
  • Vaccinia vectors and methods useful in immunization protocols are described in, e.g., U.S. Patent No. 4,722,848 .
  • Another suitable vector is BCG (Bacille Calmette Guerin).
  • BCG vectors are described in Stover CK, et al., (1991) Nature 351:456-60 .
  • vectors useful for therapeutic administration or immunization e.g., adeno and adeno-associated virus vectors, retroviral vectors, Salmonella typhi vectors, detoxified anthrax toxin vectors, and the like, are known in the art. See, e.g., Shata MT, et al., (2000) Mol. Med. Today 6:66-71 ; Shedlock DJ and Weiner DB., et al., (2000) J. Leukoc. Biol. 68:793-806 ; and Hipp JD, et al., (2000) In Vivo 14:572-85 .
  • the present invention also provides methods of inducing antigen-presenting cells using one or more peptides of this invention.
  • the antigen-presenting cells can be induced by inducing dendritic cells from the peripheral blood monocytes and then contacting (stimulating) them with one or more peptides of this invention in vitro, ex vivo or in vivo.
  • peptides of the present invention are administered to the subjects, antigen-presenting cells that have the peptides of this invention immobilized to them are induced in the body of the subject.
  • the cells can be administered to the subject as a vaccine.
  • the ex vivo administration may include the steps of:
  • the present invention use of the peptides of this invention for manufacturing a pharmaceutical composition inducing antigen-presenting cells is provided. Further, the present invention also provides the peptide of the present invention for inducing antigen-presenting cells.
  • the antigen-presenting cells obtained by step b can be administered to the subject as a vaccine.
  • This invention also provides a method for inducing antigen-presenting cells having a high level of cytotoxic T cell inducibility, in which the method includes the step of transferring genes composed of polynucleotide(s) encoding one or more peptides of this invention to antigen-presenting cells in vitro.
  • the introduced genes may be in the form of DNAs or RNAs.
  • various methods conventionally performed in this field such as lipofection, electroporation, and calcium phosphate method may be suitably used. More specifically, transfection may be performed as described in Reeves ME, et al., (1996) Cancer Res., 56:5672-7 .; Butterfield LH, et al., (1998) J.
  • the present invention further provides methods for inducing CTL using one or more peptides of this invention.
  • CTL are induced in the body of the subject, and the strength of the immune system targeting the cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer cells in the tumor tissues is thereby enhanced.
  • the cancers contemplated include, but are not limited to bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the peptides of the present invention may be used in the context of an ex vivo therapeutic method, in which subject-derived antigen-presenting cells and CD8-positive cells or peripheral blood mononuclear leukocytes are contacted (stimulated) with one or more peptides of this invention in vitro, and, after inducing CTL, the cells are returned to the subject.
  • the method may include the steps of:
  • the present invention use of the peptides of this invention for manufacturing a pharmaceutical composition inducing CTLs is provided. Further, the present invention also provides the peptide of the present invention for inducing CTLs.
  • the CD 8+ T cells having cytotoxic activity obtained by step d can be administered to the subject as a vaccine.
  • the present invention further provides isolated cytotoxic T cells induced using the peptides of this invention.
  • the cytotoxic T cells induced by stimulation with an antigen-presenting cell presenting one or more peptides of this invention, are preferably derived from subjects who are the target of treatment and/or prevention, and can be administered alone or in combination with other drugs, including one or more peptides of this invention or exosomes having anti-tumor activity.
  • the obtained cytotoxic T cells act specifically against target cells presenting the peptides of this invention, or preferably the same peptide(s) used for induction.
  • the target cells may be cells that express CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 endogenously, or cells that are transfected with CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 genes.
  • Cells that present the peptides of this invention on the cell surface, due to stimulation with these peptides, can also become targets of attack.
  • the present invention also provides antigen-presenting cells presenting complexes formed between HLA antigens and one or more peptides of this invention.
  • the antigen-presenting cells obtained through contact with the peptides of this invention or the nucleotides encoding such peptides, are preferably derived from subjects who are the target of treatment and/or prevention, and can be administered as vaccines, alone or in combination with other drugs, including the peptides, exosomes, or cytotoxic T cells of the present invention.
  • the present invention also provides a composition composed of nucleic acids encoding polypeptides that are capable of forming a subunit of a T cell receptor (TCR), and methods of using the same.
  • TCR subunits have the ability to form TCRs that confer specificity to T cells for tumor cells presenting CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10.
  • the nucleic acids of alpha- and beta-chain as the TCR subunits of the CTL induced with one or more peptides of this invention may be identified ( WO2007/032255 and Morgan et al., J Immunol, 171, 3288 (2003 )).
  • the derivative TCRs preferably bind target cells displaying the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide with high avidity, and optionally mediate efficient killing of target cells presenting the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide in vivo and in vitro.
  • the nucleic acids encoding the TCR subunits can be incorporated into suitable vectors e.g. retroviral vectors. These vectors are well known in the art.
  • the nucleic acids or the vectors containing them usefully can be transferred into a T cell, which T cell is preferably from a patient.
  • the invention provides an off-the-shelf composition allowing rapid modification of a patient's own T cells (or those of another mammal) to rapidly and easily produce modified T cells having excellent cancer cell killing properties.
  • the present invention provides CTLs which are prepared by transduction with the nucleic acids encoding the TCR subunits polypeptides binding with CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide e.g.
  • the transduced CTLs are capable of homing to cancer cells in vivo, and expanded by well known culturing method in vitro (e.g., Kawakami et al., J Immunol., 142, 3452-3461 (1989 )).
  • the T cells of the invention can be used to form an immunogenic composition useful in treating or preventing cancer in a patient in need of therapy or protection ( WO2006/031221 ).
  • the term "vaccine” refers to a substance that induces anti-tumor immunity or suppresses cancers upon inoculation into animals.
  • polypeptides having the amino acid sequence of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223 were suggested to be HLA-A24 restricted epitope peptides and those of SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288 were suggested to be HLA-A2 restricted epitope peptides that may induce potent and specific immune response against cells expressing CDH3, EPHA
  • the cancers contemplated include, but are not limited to bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the present invention also encompasses a method of inducing anti-tumor immunity using polypeptides having the amino acid sequence of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 10I, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof (i.e., including 1, 2, or several (e.g., up to 5) amino acid substitutions, deletions, or additions).
  • anti-tumor immunity includes immune responses such as follows:
  • the peptide when a certain peptide induces any one of these immune responses upon inoculation into an animal, the peptide is decided to have anti-tumor immunity inducing effect.
  • the induction of the anti-tumor immunity by a peptide can be detected by observing in vivo or in vitro the response of the immune system in the host against the peptide.
  • cytotoxic T lymphocytes For example, a method for detecting the induction of cytotoxic T lymphocytes is well known.
  • a foreign substance that enters the living body is presented to T cells and B cells by the action of antigen-presenting cells (APCs).
  • APCs antigen-presenting cells
  • T cells that respond to the antigen presented by APC in antigen specific manner differentiate into cytotoxic T cells (also referred to as cytotoxic T lymphocytes or CTLs) due to stimulation by the antigen, and then proliferate; this process is referred to herein as "activation" of T cells. Therefore, CTL induction by a certain peptide can be evaluated by presenting the peptide to a T cell by APC, and detecting the induction of CTL.
  • APCs have the effect of activating CD4+ T cells, CD8+ T cells, macrophages, eosinophils and NK cells. Since CD4+ T cells are also important in anti-tumor immunity, the anti-tumor immunity inducing action of the peptide can be evaluated using the activation effect of these cells as indicators.
  • DC dendritic cells
  • APC dendritic cells
  • DC is a representative APC having the strongest CTL inducing action among APCs.
  • the test polypeptide is initially contacted with DC and then this DC is contacted with T cells. Detection of T cells having cytotoxic effects against the cells of interest after the contact with DC shows that the test polypeptide has an activity of inducing the cytotoxic T cells.
  • Activity of CTL against tumors can be detected, for example, using the lysis of 51 Cr-labeled tumor cells as the indicator.
  • peripheral blood mononuclear cells may also be used as the APC.
  • the induction of CTL is reported to be enhanced by culturing PBMC in the presence of GM-CSF and IL-4.
  • CTL has been shown to be induced by culturing PBMC in the presence of keyhole limpet hemocyanin (KLH) and IL-7.
  • KLH keyhole limpet hemocyanin
  • test polypeptides confirmed to possess CTL inducing activity by these methods are polypeptides having DC activation effect and subsequent CTL inducing activity. Therefore, polypeptides that induce CTL against cells expressed CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 are useful as vaccines against diseases associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers.
  • APC that have acquired the ability to induce CTL against a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, by contacting with the polypeptides are useful as vaccines against the disease.
  • CTL that have acquired cytotoxicity due to presentation of the polypeptide antigens by APC can be also used as vaccines against a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers.
  • Such therapeutic methods for a disease associating CDH3, EPHA4, ECT2, HTG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 are referred to as cellular immunotherapy.
  • the cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the induction of anti-tumor immunity by a polypeptide can be further confirmed by observing the induction of antibody production against tumors. For example, when antibodies against a polypeptide are induced in a laboratory animal immunized with the polypeptide, and when growth, proliferation and/or metastasis of tumor cells is suppressed by those antibodies, the polypeptide is determined to induce anti-tumor immunity.
  • Anti-tumor immunity can be induced by administering a vaccine of this invention, and the induction of anti-tumor immunity enables treatment and prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. Therapy against or prevention of the onset of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g.
  • cancers may include inhibition of the growth of cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer cells, involution of these cells and suppression of occurrence of these cells, e.g. cancer cells.
  • Decrease in mortality of individuals having a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 e.g. cancers, decrease of the disease markers in the blood, alleviation of detectable symptoms accompanying the disease and such are also included in the therapy or prevention of the disease, e.g. cancers.
  • Such therapeutic and preventive effects are preferably statistically significant, for example, observed at a significance level of 5% or less, wherein the therapeutic or preventive effect of a vaccine against a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, is compared to a control without vaccine administration.
  • a vaccine against a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 e.g. cancers
  • Student's t-test the Mann-Whitney U-test or ANOVA may be used for determining statistical significance.
  • the present invention provides a method for treating, or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers
  • the therapeutic compounds or compositions may be administered prophylactically or therapeutically to subjects suffering from or at risk of (or susceptible to) developing the disease. Such subjects may be identified using standard clinical methods.
  • prophylactic administration occurs prior to the manifestation of overt clinical symptoms of disease, such that a disease or disorder is prevented or alternatively delayed in its progression.
  • the term "prevent" encompasses any activity which reduces the burden of mortality or morbidity from disease.
  • Prevention can occur at primary, secondary and tertiary prevention levels. While primary prevention avoids the development of a disease, secondary and tertiary levels of prevention encompass activities aimed at preventing the progression of a disease and the emergence of symptoms as well as reducing the negative impact of an already established disease by restoring function and reducing disease-related complications.
  • the term “efficacious” refers to a treatment that leads to a decrease in size, prevalence or metastatic potential of cancer in a subject.
  • “efficacious” means that the treatment retards or prevents occurrence of non cancer or alleviates a clinical symptom of cancer.
  • the assessment of cancer can be made using standard clinical protocols.
  • the efficaciousness of a treatment may be determined in association with any known method for diagnosing or treating cancer. For example, cancer can be diagnosed histopathologically or by identifying symptomatic anomalies.
  • peptide having immunological activity, or a polynucleotide or vector encoding such a peptide
  • An adjuvant refers to a compound that enhances the immune response against the peptide when administered together (or successively) with the peptide having immunological activity.
  • suitable adjuvants include cholera toxin, salmonella toxin, alum and such, but are not limited thereto.
  • a vaccine of this invention may be combined appropriately with a pharmaceutically acceptable carrier. Examples of such carriers are sterilized water, physiological saline, phosphate buffer, culture fluid and such.
  • the vaccine may contain as necessary, stabilizers, suspensions, preservatives, surfactants and such.
  • the vaccine is administered systemically or locally.
  • Vaccine administration may be performed by single administration or boosted by multiple administrations.
  • a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 can be treated or prevented, for example, by the ex vivo method. More specifically, PBMCs of the subject receiving treatment or prevention are collected, contacted ex vivo with a peptide of the present invention. Following the induction of APC or CTL, the cells may be administered to the subject.
  • APC can be also induced by introducing a vector encoding the peptide into PBMCs ex vivo. APC or CTL induced in vitro can be cloned prior to administration.
  • APC and CTL isolated in this manner may be used for cellular immunotherapy not only against individuals from whom the cells are derived, but also against similar types of diseases in other individuals.
  • A24-LCL cells (HLA-A24), human B-lymphoblastoid cell line, was established by transforming with Epstain-bar virus.
  • T2 cell, COS7, A498, Caki-2 and HEK 293 were purchased from ATCC.
  • Caki-1 and MIAPaca-2 were purchased from JCRB.
  • PK-45P, PK-59, TE-5 and TE-6 were purchased from TKG. 293 T was purchased from GenHunter.
  • peptides were synthesized by Sigma (Sapporo, Japan) according to the standard solid phase synthesis method and purified by reversed phase HPLC. The purity (>90%) and the identity of the peptides were determined by analytical HPLC and mass spectrometry analysis, respectively. Peptides were dissolved in dimethylsulfoxide (DMSO) at 20 mg/ml and stored at -80 degrees C.
  • DMSO dimethylsulfoxide
  • DCs Monocyte-derived dendritic cells
  • APCs antigen-presenting cells
  • peripheral blood mononuclear cells isolated from a normal volunteer (HLA-A*2402 and/or HLA-A*0201) by Ficoll-Paque (Pharmacia) solution were separated by adherence to a plastic tissue culture flask (Becton Dickinson) so as to enrich them for the monocyte fraction.
  • the monocyte-enriched population was cultured in the presence of 1000 U/ml of GM-CSF (Genzyme) and 1000 U/ml of IL-4 (Genzyme)in AIM-V(Invitrogen)containing 2% heat-inactivated autologous serum (AS).
  • the cytokine-generated DCs were pulsed with 20 micro g/ml of the synthesized peptides in the presence of 3 micro g/ml of beta 2-microglobulin for 4 hrs at 20 degrees C in AIM-V. These peptide-pulsed DCs were then inactivated by MMC (30micro g/ml for 30 mins) and mixed at a 1:20 ratio with autologous CD8 + T cells, obtained by positive selection with Dynabeads M-450 CD8 (Dynal) and DETACHa BEADTM (Dynal).
  • CTLs were expanded in culture using the method similar to that described by Riddell SR, et al., ( Walter EA et al., (1995) N Engl J Med 333:1038-44 .; Riddel SR, et al., (1996) Nature Med. 2:216-23 .).
  • a total 5x10 4 of CTLs were resuspended in 25 ml of AIM-V/5% AS with 2 kinds of human B-lymphoblastoid cell lines, inactivated by MMC, in the presence of 40 ng/ml of anti-CD3 monoclonal antibody (Pharmingen).
  • 120 IU/ml of IL-2 were added to the cultures.
  • the cultures were fed with fresh AIM-V/5% AS containing 30 IU/ml of IL-2 on days 5, 8 and 11.
  • CTLs were cultured with 7x10 4 cells/well of 2 kinds of human B-lynphoblastoid cell lines, 30ng/ml of anti-CD3 antibody, and 125 U/ml of IL-2 in total of 150micro 1/well of AIM-V containing 5%AS. 50 micro 1 /well of IL-2 was added to the medium 10 days later so that IL-2 became 125 U/ml in the final concentration. CTL activity of CTLs was tested on the 14th day, and CTL clones were expanded using the same method above.
  • IFN-gamma ELISPOT assay To examine the specific CTL activity, IFN-gamma ELISPOT assay and IFN-gamma ELISA assay were performed.
  • peptide-pulsed A24-LCL or T2 cell (1x10 4 /well) was prepared as stimulator cells.
  • Cultured Cells in 48 wells or CTL clones after limiting dilution were used as responder cells.
  • IFN-gamma ELISPOT assay and ELISA assay were performed under manufacture procedure.
  • the cDNA encoding an open reading frame of taget genes or HLA-A02 or HLA-A24 was amplified by PCR.
  • the PCR-amplified product was cloned into pcDNA3.1 myc-His vector (Invitrogen).
  • the plasmids were transfected into the taget genes, HLA-A02 and HLA-A24-null nomal human cell line COS7 or 293T using lipofectamine (Invitrogen) according to the manufacturer's recommended procedures.
  • the plasmid contained the target genes were transfected into A24-LCL by electroporation using GenePulserII (Biorad).
  • A24-LCL cells were pulsed with 10 mcg prasmid at 140V and 1000 micro F. After 2days from transfection, the transfected cells were treated with Cell dissociation solution and used as the target cells for CTL activity assay.
  • Cytotoxic activity was evaluated by a four-hour 51 Cr release assay.
  • the target cells were pulsed with a 20 micro g/mL concentration of peptide overnight.
  • the target cells were labeled with 100 micro Ci of Na 2 51 CrO 4 at 37 degrees C for one hour, and then washed three times with RPMI1640.
  • the target cells (1x 10 4 /100 micro L) and 100 micro L of effector cells at various numbers with a total volume of 200 micro L were placed into a round-bottomed 96-well microtiter plate (Coming), and cultured at 37 degrees C in a CO 2 incubator for four hours. After culturing, 100 micro L of the supernatant was collected from each well, and measured the radioactivity using a gamma counter. Spontaneous release was the radioactivity from the target cells with medium in the absence of effector cells, and maximum releasewas the radioactivity from the target cells with 1 M HCl.
  • % Specific lysis experimental release - spontaneous release / maximum release - spontaneous release ⁇ x 100.
  • CDH3 GenBank Accession No. NM_001793; SEQ ID Nos.1 2
  • EPHA4 GeneBank Accession No. L36645; SEQ ID Nos.3, 4
  • ECT2 GeneBank Accession No. AY376439; SEQ ID Nos.5, 6
  • HIG2 GenBank Accession No. NM_013332; SEQ ID Nos.7, 8
  • INHBB GenBank Accession No. NM_002193; SEQ ID Nos.9, 10
  • KIF20A GenBank Accession No.
  • NM_005733 SEQ ID Nos.11, 12
  • KNTC2 GenBank Accession No. AF017790; SEQ ID Nos.13, 14
  • TTK GenBank Accession No. NM_003318; SEQ ID Nos.15, 16
  • URLC10 GenBank Accession No. NM_017527; SEQ ID Nos.17, 18
  • EPHA4 expression was validly elevated in the following cancers in comparison with corresponding normal tissue.
  • ECT2 expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
  • INHBB expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
  • KIF20A expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
  • KNTC2 expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
  • TTK expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
  • URLC10 expression was validly elevated in the following cancers in comparing with corresponding normal tissue
  • Table 2 sets forth the HLA-A*2402 binding peptides for CDH3 in order of binding affinity.
  • Table 2A sets forth 9-mer peptides derived from CDH3 and
  • Table 2B sets forth 10-mer peptides derived from CDH3.
  • Table 3 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for EPHA4 in order of binding affinity.
  • Table 3A sets forth the HLA-A*2402 binding 9-mer peptides derived from EPHA4
  • Table 3B shows the HLA-A*2402 binding 10-mer peptides derived from EPHA4
  • Table 3C sets forth the HLA-A*0201 binding 9-mer peptides derived from EPHA4.
  • Table 4 sets forth the HLA-A*2402 binding peptides for ECT2 in order of binding affinity.
  • Table 4A sets forth 9-mer peptides derived from ECT2 and
  • Table 4B shows 10-mer peptides derived from ECT2.
  • Table 5 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for HIG2
  • Table 5A sets forth the HLA-A*2402 binding 9-mer peptides derived from HIG2
  • Table 5B sets forth the HLA-A*2402 binding 10-mer peptides derived from HIG
  • Table 5C sets forth the HLA-A*0201 binding 9-mer peptides derived from HIG2
  • Table 5D sets forth HLA-A*0201 binding 10-mer peptides derived from HIG2.
  • Table 6 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for INHBB
  • Table 6A shows the HLA-A*2402 binding 9-mer peptides derived from INHBB
  • Table 6B sets forth the HLA-A*2402 binding 10-mer peptides derived from INHBB
  • Table 6C sets forth the HLA-A*0201 binding 9-mer peptides derived from INHBB
  • Table 6D sets forth HLA-A*0201 binding 10-mer peptides derived from INHBB.
  • Table 7 sets forth the HLA-A*2402 binding peptides for KIF20A in order of binding affinity.
  • Table 7A sets forth 9-mer peptides derived from KIF20A and
  • Table 7B sets forth 10-mer peptides derived from KIF20A.
  • Table 8 sets forth the HLA-A*2402 binding peptides for KNTC2 in order of binding affinity.
  • Table 8A sets forth 9-mer peptides derived from KNTC2 and
  • Table 8B sets forth 10-mer peptides derived from KNTC2.
  • Table 9 sets forth the HLA-A*0201 binding peptides for TTK in order of binding affinity.
  • Table 9A sets forth 9-mer peptides derived from TTK and
  • Table 9B sets forth 10-mer peptides derived from TTK.
  • Table 10 sets forth the HLA-A*0201 binding peptides for URLC10 in order of binding affinity.
  • Table 10A sets forth 9-mer peptides derived from URLC10 and
  • Table 10B sets forth 10-mer peptides derived from URLC10.
  • Start position indicates the number of amino acid from N-terminal.
  • Binding score is derived from "BIMAS” described in Materials and Methods.
  • Positive donor number indicates the number of doner whoes CD8+-T-cells can be induced to the specific CTL by the ex vivo stimulation with antigen-presenting cells. This is shown as (positive donor number / hole donor nember).
  • Positive well number indicates the number of wells where specific IFN-gamma production can be detected by IFN-gamma ELISPOT assay. 4 to 8 wells can be prepared from one donor. This is shown as (positive wells number / the number of hole wells tested by IFN-gamma ELISPOT assay).
  • Positve CTL line indicates the number of CTL line established from positive wells. The generation of CTL line is determined by ELISA. This is shown as (established CTL line number / the number of positive wells tested by IFN-gamma ELISPOT assay).
  • No positive donor is not defined by no detectable positive wells, but by no established CTL line.
  • the peptides showed by bold character in tables possesses the stimulation activity of the T cells.
  • CTLs for those peptides derived from CDH3 were generated according to the protocols set forth in "Materials and Methods" section above. Resulting that CTLs having detectable specific CTL activity, as determined by IFN-gamma ELISPOT assay, are shown in Figure 1 .
  • CDH3-A24-9-513 SEQ ID NO: 19
  • CDH3-A24-9-406 SEQ ID NO: 22
  • CDH3-A24-10-807 SEQ ID NO: 30
  • CDH3-A24-10-332 SEQ ID NO: 34.
  • CDH3-A24-10-655 SEQ ID NO: 344) and CDH3-A24-10-470 (SEQ ID NO: 358) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and the cells in the positive well number #5 stimultaed with SEQ ID NO: 19, #2 with SEQ ID NO: 22, #5 with SEQ ID NO: 30, #4 with SEQ ID NO: 34, #1 with SEQ ID NO: 344 and #4 with SEQ ID NO: 358 were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 1 .
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing CDH3 and HLA-A*2402.
  • Specific CTL activity against COS7 transfected with both full length CDH3 gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express CDH3 and HLA-A*2402 was tested using as effector cells the CTL lines raised by CDH3-A24-10-807 (SEQ ID NO: 30) and CDH3-A24-10-655 (SEQ ID NO: 344).
  • COS7 transfected with full length CDH3 but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length CDH3 were prepared as controls.
  • the CTL clones demonstrating the highest specific CTL activity against COS7 was that transfected with both CDH3 and HLA-A2402 ( Figure If and g).
  • CDH3-A24-10-807 SEQ ID NO: 30
  • CDH3-A24-10-655 SEQ ID NO: 344
  • these peptides are epitope peptides, which may serve as cancer vaccines targeting CDH3 expressed tumors.
  • CTLs for those peptides derived from EphA4 were generated by IFN-gamma ELISPOT assay. Resulting that CTLs having detectable specific CTL activity , as determined by IFN-gamma ELISPOT assay, are shown in Figure 2 .
  • EphA4-A24-9-453 (SEQ ID NO: 41), EphA4-A24-9-S (SEQ ID NO: 44), EphA4-A24-9-869 (SEQ ID NO: 46), EphA4-A24-9-420 (SEQ ID NO: 48), EphA4-A24-10-24 (SEQ ID NO: 78), EphA4-A02-9-501 (SEQ ID NO: 376) and EphA4-A02-9-165 (SEQ ID NO: 379) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #3 stimultaed with EphA4-A24-9-453 (SEQ ID NO: 41), #2 with EphA4-A24-9-5 (SEQ ID NO: 44), #5 with EphA4-A24-9-869 (SEQ ID NO: 46), #6 with EphA4-A24-9-420 (SEQ ID NO: 48), #4 with EphA4-
  • CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA.
  • CTL lines stimulated with EphA4-A02-9-501 (SEQ ID NO: 376) and EphA4-A02-9-165 (SEQ ID NO: 379) were tested by 51Cr-release assay according to the protocols set forth in the "Materials and Methods" section above. Results are shown in Figure 2a-h . While, other peptides shown in table 3 could not establish the CTL lines despite possible binding activity with HLA-A*2402 or HLA-A*0201. For example, the tipical negative peptide (EphA4-A24-9-384) were shown in Figure 2a . In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputides.
  • CTLs for those peptides derived from ECT2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 3 ,.
  • ECT2-A24-9-515 SEQ ID NO: 80
  • ECT2-A24-10-40 SEQ ID NO: 100
  • ECT2-A24-10-101 SEQ ID NO: 101
  • the cells in the positive well number #7 stimultaed with ECT2-A24-9-515 SEQ ID NO: 80
  • #2 with ECT2-A24-10-40 SEQ ID NO: 100
  • #1 with ECT2-A24-10-101 SEQ ID NO: 101
  • CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 3a-d .
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing ECT2 and HLA-A*2402.
  • Specific CTL activity against COS7 transfected with both full length ECT2 gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express ECT2 and HLA-A*2402 was tested using as effector cells the CTL clone raised by ECT2-A24-10-40 (SEQ ID NO: 100) and the CTL line raised by ECT2-A24-10-101 (SEQ ID NO: 101).
  • COS7 transfected with full length ECT2 but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length ECT2 were prepared as controls.
  • the CTL line demonstrating the highest specific CTL activity against COS7 was that transfected with both ECT2 and HLA-A2402 ( Figure 3c and d ).
  • ECT2-A24-10-40 SEQ ID NO: 100
  • ECT2-A24-10-101 SEQ ID NO: 101
  • these peptides are epitope peptides, which may serve as cancer vaccines targeting ECT2 expressed tumors.
  • Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above.
  • CTL clone stimulated with ECT2-A24-9-515 SEQ ID NO: 80
  • ECT2-A24-9-515 SEQ ID NO: 80
  • CTLs for those peptides derived from HIG2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 4 .
  • HIG2-A24-9-19 SEQ ID NO: 110
  • HIG2-A24-9-22 SEQ ID NO: 111
  • HIG2-A24-9-8 SEQ ID NO: 387
  • HIG2-A24-10-7 SEQ ID NO: 112
  • HIG2-A24-10-18 SEQ ID NO: 394
  • HIG2-A02-9-8 SEQ ID NO: 114
  • HIG2-A02-9-15 SEQ ID NO: 116
  • HIG2-A02-9-4 SEQ ID NO: 117
  • HIG2-A02-10-8 SEQ ID NO: 121
  • the cells in the positive well number #6 stimultaed with HIG2-A24-9-19 SEQ ID NO: 110
  • #7 with HIG2-A24-9-22 SEQ ID NO: 111
  • #5 with HIG2-A24-9-8 SEQ ID NO: 387
  • #1 with HIG2-A24-10-7 SEQ ID NO: 11
  • CTL clones were potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing HIG2 and HLA-A*0201.
  • HIG2-A02-9-8 (SEQ ID NO: 114), HIG2-A02-9-15 (SEQ ID NO: 116) and HIG2-A02-9-4 (SEQ ID NO: 117) are naturally expressed on the target cell surface with HLA-A2402 or HLA-A0201 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting HIG2 expressed tumors.
  • Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above.
  • CTL clone stimulated with HIG2-A02-9-4 SEQ ID NO: 117
  • CTLs for those peptides derived from INHBB were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 5 .
  • TNHBB-A24-9-180 (SEQ ID NO: 395), INHBB-A24-10-180 (SEQ ID NO: 133), INHBB-A24-10-305 (SEQ ID NO: 135), INHBB-A24-10-7 (SEQ ID NO: 137) and INHBB-A24-10-212 (SEQ ID NO: 426) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #7 stimultaed with INHBB-A24-9-180 (SEQ ID NO: 395), #3 with INHBB-A24-10-180 (SEQ ID NO: 133), #2 with INHBB-A24-10-305 (SEQ ID NO: 135), #8 and #2 with INHBB-A24-10-7 (SEQ ID NO: 137) and #1 with INHBB-A24-10-212 (SEQ ID NO: 426) were expanded and CTL lines were established.
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing INHBB and HLA-A*2402.
  • Specific CTL activity against 293T transfected with both full length INHBB gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express INHBB and HLA-A*2402 was tested using as effector cells the CTL lines raised by INHBB-A24-10-180 (SEQ ID NO: 133) and INHBB-A24-10-7 (SEQ ID NO: 137) and the CTL clone raised by INHBB-A24-10-305 (SEQ ID NO: 135),.
  • INHBB-A24-10-305 SEQ ID NO: 135)
  • INHBB-A24-10-180 SEQ ID NO: 133
  • INHBB-A24-10-7 SEQ ID NO: 137
  • these peptides are epitope peptides, which may serve as cancer vaccines targeting INHBB expressed tumors.
  • Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above.
  • CTL clone stimulated with INHBB-A24-9-180 SEQ ID NO: 395
  • CTLs for those peptides derived from KIF20A were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 6 .
  • KIF20A-A24-9-305 (SEQ ID NO: 174), KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-304 (SEQ ID NO: 186) and KIF20A-A24-10-66 (SEQ ID NO: 194) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #2 stimultaed with KIF20A-A24-9-305 (SEQ ID NO: 174), #3 with KIF20A-A24-9-383 (SEQ ID NO: 178), #5 with KIF20A-A24-10-304 (SEQ ID NO: 186) and #6 with KIF20A-A24-10-66 (SEQ ID NO: 194) were expanded and CTL lines were established.
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing KIF20A and HLA-A*2402.
  • Specific CTL activity against COS7 transfected with both full length KIF20A gene and the HLA-A*2402 molecule and A24-LCL transfected by electropolation with full length KIF20A gene, which serve as a specific model for the target cells endogenously express KIF20A and HLA-A*2402 was tested using as effector cells the CTL lines raised by KIF20A-A24-9-383 (SEQ ID NO: 178) and KIF20A-A24-10-304 (SEQ ID NO: 186) and the CTL clone raised by KIF20A-A24-10-66 (SEQ ID NO: 194).
  • COS7 transfected with full length KIF20A but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length KIF20A (or replaced full length URLC10 gene), COS7 transfected with HLA-A*2-402 and pulesd with KIF20A-10-308, and A24-LCL transfected with mock vector were prepared as controls.
  • the CTL line demonstrating the highest specific CTL activity against COS7 was that transfected with both KIF20A and HLA-A*2402 ( Figure 6b, c and d ).
  • the CTL line stuimurated with KIF20A-A24-10-304 demonstrated against A24-LCL transfected with KIF20A.
  • KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-304 (SEQ ID NO: 186) and KIF20A-A24-10-66 (SEQ ID NO: 194) is naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL.
  • these peptides are epitope peptides, which may serve as cancer vaccines targeting KIF20A expressed tumors.
  • Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above.
  • CTL clone stimulated with KIF20A-A24-9-305 (SEQ ID NO: 174) or KIF20A-A24-10-304 (SEQ ID NO: 186) showed remarkably high cytotoxic effect towards HLA-A24-positive and KIF20A-positive cancer cell lines PK45P or MIAPaca2 respectively, compared to that towards HLA-A24-negative and KIF20A-positive cancer cell lines PK59.
  • CTLs for those peptides derived from KNTC2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 7 .
  • KNTC2-A24-9-309 SEQ ID NO: 196
  • KNTC2-A24-9-124 SEQ ID NO: 202
  • KNTC2-A24-9-154 SEQ ID NO: 210
  • KNTC2-A24-9-150 SEQ ID NO: 213)
  • KNTC2-A24-10-452 SEQ ID NO: 214
  • KNTC2-A24-10-227 SEQ ID NO: 217)
  • KNTC2-A24-10-273 SEQ ID NO: 223
  • the cells in the positive well number #8 stimultaed with KNTC2-A24-9-309 SEQ ID NO: 196
  • #5 with KNTC2-A24-9-124 SEQ ID NO: 202
  • #5 with KNTC2-A24-9-154 SEQ ID NO: 210
  • #7 with KNTC2-A24-9-150 SEQ ID NO: 213)
  • #4 and #5 with KNTC2-A24-9-150 SEQ ID NO: 213
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing KNTC2 and HLA-A*2402.
  • HEK293 transfected with full length KNTC2 but not HLA-A*2402 HEK293 transfected with HLA-A*2402 but not full length KNTC2 and HEK293 transfected with HLA-A*2402 and pulesd with KNTC2-9-309 were prepared as controls.
  • the CTL line demonstrating the highest specific CTL activity against HEK293 was that transfected with both KNTC2 and HLA-A*2402 ( Figure 7f ).
  • KNTC2-A24-1O-452 (SEQ ID NO: 214) is naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting KNTC2 expressed tumors.
  • CTLs for those peptides derived from TTK were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 8 .
  • TTK-A2-9-462 (SEQ ID NO: 227), TTK-A2-9-547 (SEQ ID NO: 228), TTK-A2-9-719 (SEQ ID NO: 233) and TTK-A2-10-462 (SEQ ID NO: 254) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #4 stimultaed with TTK-A2-9-462 (SEQ ID NO: 227), #2 with TTK-A2-9-547 (SEQ ID NO: 228), #1 with TTK-A2-9-719 (SEQ ID NO: 233) and #8 with TTK-A2-10-462 (SEQ ID NO: 254) were expanded.
  • CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. While, other peptides shown in table 9 could not establish the CTL lines despite possible binding activity with HLA-A*0201. For example, the tipical negative peptide (TTK-A2-9-278) were shown in Figure 8a . In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
  • CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
  • the established CTL clone raised against these peptides were examined for their ability to recognize the target cells endogenously expressing TTK and HLA-A*0201.
  • Specific CTL activity against COS7 transfected with both the full length TTK gene and the HLA-A*0201 molecule which is a specific model for the target cells endogenously express TTK and HLA-A*0201, was tested using as effector cells the CTL clones raised by TTK-A2-9-462 (SEQ ID NO: 227), TTK-A02-9-547 (SEQ ID NO: 228), TTK-A2-9-719 (SEQ ID NO: 233) and TTK-A2-10-462 (SEQ ID NO: 254).
  • the CTL Clone having the highest specific CTL activity against COS7 was that transfected with both TTK and HLA-A*0201 ( Figure 8b, c, d and e ).
  • TTK-A2-9-462 SEQ ID NO: 227)
  • TTK-A02-9-547 SEQ ID NO: 2248
  • TTK-A2-9-719 SEQ ID NO: 233
  • TTK-A02-10-462 SEQ ID NO: 254
  • these peptides are epitope peptides, which may serve as cancer vaccines targeting TTK expressed tumors.
  • CTLs for those peptides derived from URLC10 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by IFN-gamma ELISPOT assay are shown in Figure 9 .
  • URLC-A2-9-206 (SEQ ID NO: 271), URLC-A2-9-212 (SEQ ID NO: 272) and URLC-A2-10-211 (SEQ ID NO: 288) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #7 stimultaed with URLC-A2-9-206 (SEQ ID NO: 271), #3 with URLC-A2-9-212 (SEQ ID NO: 272) and #5 with URLC-A2-10-211 (SEQ ID NO: 288) were expanded.
  • CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. While, other peptides shown in table 10 could not establish the CTL lines despite possible binding activity with HLA-A*0201. For example, the tipical negative peptide (URLC-A2-9-58) were shown in Figure 9a . In this invention, the peptiede which could establish CTL line were selected as potent CTL stimulation peputide.
  • the established CTL line raised against these peptides were examined for their ability to recognize the target cells endogenously expressing URLC10 and HLA-A*0201.
  • COS7, Hek293 or 293T transfected with full length URLC10 but not HLA-A*0201 (replaced HLA-A*2402), COS7, Hek293 or 293T transfected with HLA-A*0201 but not full length URLC10 and COS7 transfected with HLA-A*0201 and pulsed with different target epitope peputide (URLC10-A02-10-64) were prepared as controls.
  • the CTL line demonstrating the highest specific CTL activity against COS7, Hek293 or 293T was that transfected with both URLC10 and HLA-A*0201 ( Figure 9-2 ).
  • cDNA microarray technologies can disclose comprehensive profiles of gene expression of malignant cells ( Lin YM, et al., Oncogene. 2002 Jun 13;21:4120-8 .; Kitahara O, et al., Cancer Res. 2001 May 1;61:3544-9 .; Suzuki C, et al., Cancer Res. 2003 Nov 1;63:7038-41 .; Ashida S, Cancer Res. 2004 Sep 1;64:5963-72 .; Ochi K, et al., Int J Oncol. 2004 Mar;24(3):647-55 .; Kaneta Y, et al., Int J Oncol. 2003 Sep;23:681-91 .; Obama K, Hepatology.
  • CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 are over-expressed in various cancers but show minimal expression in normal tissues. In addition, these genes have been shown to have a significant function related to cell proliferation. Thus, peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 can serve as TAA epitopes, which, in turn, can be used to induce significant and specific immune responses against cancer cells.
  • CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 are novel TAAs, vaccines using these epitope peptides find utility as immunotherapeutics against various carcinomas or other disease expressing these molecules.
  • the present invention identifies new TAAs, particularly those which induce potent and specific anti-tumor immune responses. Such TAAs warrants further development as peptide vaccines against diseases associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or, URLC10 e.g. cancers.
  • the present invention relates to the following items:
  • the peptide of item 2 wherein the second amino acid from the N-terminus is phenylalanine, tyrosine, methionine, or tryptophan.
  • the peptide of item 2 or 3, wherein the C-terminal amino acid is phenylalanine, leucine, isoleucine, tryptophan, or methionine.
  • An isolated peptide of less than about 15 amino acids selected from the group consisting of peptides comprising the amino acid sequences of SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288.
  • a peptide having cytotoxic T cell inducibility comprising an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288, wherein 1, 2, or several amino acids are substituted, deleted, or added.
  • a pharmaceutical composition for treating or preventing a disease associated with over-expression of the genes of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 said composition comprising one or more peptides of item 1 to 8 or a polynucleotide encoding the peptides.
  • An exosome that presents on its surface a complex comprising a peptide of item 1-4 and an HLA antigen.
  • An exosome that presents on its surface a complex comprising a peptide of item 5-8 and an HLA antigen.
  • a method of inducing antigen-presenting cells having a high cytotoxic T cell inducibility comprising the step of contacting an antigen-presenting cell with a peptide of item 1-8.
  • a method of inducing cytotoxic T cells by contacting a T cell with a peptide of item 1-8.
  • a method of inducing antigen-presenting cells having high cytotoxic T cell inducibility comprising the step of transferring a gene comprising a polynucleotide encoding a peptide of item 1 to 8 to an antigen-presenting cell.
  • An isolated cytotoxic T cell which is induced by contacting a T cell with a peptide of item 1-8 or which is transduced with the nucleic acids encoding the TCR subunits polypeptides binding with a peptide of any one items 1 to 8 in the context of HLA-A24 or HLA-A2.
  • An antigen-presenting cell which comprises a complex formed between an HLA antigen and a peptide of item 1 to 8.
  • An antigen-presenting cell induced by the method of item 18-20.
  • the vaccine of item 25 wherein the cancers include bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • the vaccine of item 26 formulated for administration to a subject whose HLA antigen is HLA-A24 or HLA-A2.
  • a method of treating or preventing a disease associated with over-expression of the genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 in a subject comprising administering to said subject a vaccine comprising a peptide of item 1-8, an immunologically active fragment thereof, or a polynucleotide encoding said peptide or immunologically active fragment.
  • the method of item 28, wherein the disease associating genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 include cancers.
  • cancer cells include bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.

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Abstract

The present invention provides peptides having an amino acid sequence as set forth in SEQ ID NO: 174, 178, 186 or 194 as well as peptides having the above-mentioned amino acid sequences in which 1, 2, or several (e.g., up to 5) amino acids are substituted, deleted, or added, provided the peptides possess cytotoxic T cell inducibility. The present invention also provides drugs for treating or preventing a disease associated with over-expression of the KIF20A, e.g. cancers containing as an active ingredient one or more of these peptides. The peptides of the present invention find further utility as vaccines.

Description

    Technical Field
  • The present application claims the benefit of U.S. Provisional Application No. 60/902,949, filed February 21, 2007 , the entire disclosure of which is hereby incorporated herein by reference for all purposes.
  • The present invention relates to the field of biological science, more specifically to the field of cancer therapy. In particular, the present invention relates to novel immunogenic peptides that serve as extremely effective as cancer vaccines, and drugs for treating and preventing tumors containing such peptides.
    • Background Art
  • It has been demonstrated that CD8+ cytotoxic T lymphocytes (CTLs) recognize epitope peptides derived from tumor-associated antigens (TAAs) presented on MHC class I molecules, and subsequently lyse the tumor cells. Since the discovery of the MAGE family as the first example of TAAs, many other TAAs have been discovered using immunological approaches (Boon T. (1993) Int J Cancer 54: 177-80.; Boon T. et al., (1996) J Exp Med 183: 725-9.; van der Bruggen P et al., (1991) Science 254: 1643-7.; Brichard V et al., (1993) J Exp Med 178: 489-95.; Kawakami Y et al., (1994) J Exp Med 180: 347-52.). Some of them are now in clinical development as targets of immunotherapy. TAAs discovered to date include MAGE (van der Bruggen P et al., (1991) Science 254: 1643-7.), gp100 (Kawakami Y et al., (1994) J Exp Med 180: 347-52.), SART (Shichijo S et al., (1998) J Exp Med 187:277-88.), and NY-ESO-1 (Chen Y.T. et al., (1997) Proc. Natl. Acd. Sci. USA, 94: 1914-8.). On the other hand, certain gene products demonstrated to be somewhat specifically over-expressed in tumor cells have been shown to be recognized as targets for inducing cellular immune responses. Such gene products include p53 (Umano Y et al., (2001) Br J Cancer, 84:1052-7.), HER2/neu (Tanaka H et al., (2001) Br J Cancer, 84: 94-9.), CEA (Nukaya I et al., (1999) Int. J. Cancer 80, 92-7.) and the like.
  • Despite significant progress in basic and clinical research concerning TAAs (Rosenberg SA et al., (1998) Nature Med, 4: 321-7.; Mukherji B. et al., (1995) Proc Natl Acad Sci USA, 92: 8078-82.: Hu X et al., (1996) Cancer Res, 56: 2479-83.), only a very limited number of candidate TAAs suitable for treatment of cancers are presently available. TAAs that are abundantly expressed in cancer cells, and whose expression is restricted to cancer cells, would be promising candidates as immunotherapeutic targets.
  • Both HLA-A24 and HLA-A020 are common HLA alleles in the Japanese and Caucasian populations (Date Y et al., (1996) Tissue Antigens 47: 93-101.; Kondo A et al., (1995) J Immunol 155: 4307-12.; Kubo RT et al., (1994) J Immunol 152: 3913-24.; Imanishi et al., Proceeding of the eleventh International Histocompatibility Workshop and Conference Oxford University Press, Oxford, 1065 (1992); Williams F et al., (1997) Tissue Antigen 49: 129-33.). Thus, antigenic peptides of cancers presented by these HLA alleles may find particular utility in the treatment of cancers among Japanese and Caucasian patients. Further, it is known that the induction of low-affinity CTL in vitro usually results from exposure to high concentrations of peptides, generating a high level of specific peptide/MHC complexes on antigen-presenting cells (APCs), which will effectively activate these CTL (Alexander-Miller et al., (1996) Proc Natl Acad Sci USA 93: 4102-7.).
  • Recently, HLA class 1-binding peptide sequence can be expected using algorithms (Jounal of Immunological Methods, (1995), Vol.185, pp.181-190, J. Immunol., (1994), Vol.152, pp.163-175, protein science, (2000), Vol.9, pp.1838-1846). However, it is hard to say that the expected epitope peputide can be cut to the size and expressed on the target cell surface with HLA molecule and recognized by CTL. Moreover, the algorithm, for example BIMAS (http://bimas.dcrt.nih.gov/cgi-bin/molbio/ken_parker_comboform) (Parker KC, et al., (1994) J Immunol.;152(1):163-75.; Kuzushima K, et al., (2001) Blood.;98(6):1872-81.)) can suggest the HLA molecule-binding peputide, but the suggested peptide is not so rigorous (Bachinsky MM, et. al., Cancer Immun. 2005 Mar 22;5:6.). Thus TAA screening still remains a lot of challenges and difficulties.
  • Recent developments in cDNA microarray technologies have enabled the construction of comprehensive profiles of gene expression in malignant cells as compared to normal cells (Okabe, H. et al., (2001) Cancer Res., 61, 2129-37.; Lin YM. et al., (2002) Oncogene, 21;4120-8.; Hasegawa S. et al., (2002) Cancer Res 62:7012-7.). This approach enables a more thorough understanding of the complex nature of cancer cells and the mechanisms of carcinogenesis and facilitates the identification of genes whose expression is deregulated in tumors (Bienz M. et al., (2000) .). Among the transcripts identified as up-regulated in cancers, CDH3 (GenBank Accession No. NM_001793; SEQ ID Nos.1, 2), EPHA4 (GenBank Accession No. L36645; SEQ ID Nos.3, 4), ECT2 (GenBank Accession No. AY376439; SEQ ID Nos.5, 6), HIG2 (GenBank Accession No. NM_013332; SEQ ID Nos.7, 8) INHBB, (GenBank Accession No. NM_002193; SEQ ID Nos.9, 10), KIF20A (GenBank Accession No. NM_005733; SEQ ID Nos.11, 12), KNTC2 (GenBank Accession No. AF017790; SEQ ID Nos.13, 14), TTK (GenBank Accession No. NM_003318; SEQ ID Nos.15, 16) and URLC10 (GenBank Accession No. NM_017527; SEQ ID Nos.17, 18) have been recently discovered. The entire contents of the references are incorporated by reference herein. These genes are of particular interest to the present inventors, being specifically up-regulated in tumor cells of the various cancer tissues of the cases analyzed (see below). Thus, immunogenic peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 may find utility in selectively killing tumor cells that express such antigens. The present invention addresses these and other needs.
  • Since cytotoxic drugs, such as M-VAC, often cause severe adverse reactions, it is clear that thoughtful selection of novel target molecules on the basis of well-characterized mechanisms of action should be very helpful in the development of effective anti-cancer drugs having a minimized risk of side effects. Toward this goal, expression profile analyses were previously performed on various cancers and normal human tissue. Such studies led to the discovery of multiple genes that are specifically over-expressed in cancer (Lin YM, et al., Oncogene. 2002 Jun 13;21:4120-8.; Kitahara O, et al., Cancer Res. 2001 May 1;61:3544-9.; Suzuki C, et al., Cancer Res. 2003 Nov 1;63:7038-41.; Ashida S, Cancer Res. 2004 Sep 1;64:5963-72.; Ochi K, et al., Int J Oncol. 2004 Mar;24(3):647-55.; Kaneta Y, et al., Int J Oncol. 2003 Sep;23:681-91.; Obama K, Hepatology. 2005 Jun;41:1339-48.; Kato T, et al., Cancer Res. 2005 Jul 1;65:5638-46.; Kitahara O, et al., Neoplasia. 2002 Jul-Aug;4:295-303.; Saito-Hisaminato A et al., DNA Res 2002, 9: 35-45.). Examples of such genes identified as over-expressed in various cancers include, but are not limited to, CDH3, EPHA4, ECT2 HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10. CDH33 has been previously identified as over-expressed in bladder cancer, cervical cancer, cholangincellular carcinoma, colorectal cancer, endometriosis, gastric cancer, diffuse-type gastric cancer, non-small cell lung cancer (NSCLC), pancreatic cancer, soft tissue tumor and testicular tumor. EPHA4 has been identified in bladder cancer, cervical cancer, cholangincellular carcinoma, endometriosis, diffuse-type gastric cancer, ovarian cancer, pancreatic cancer, prostate cancer and soft tissue tumor. ECT2 has been identified in bladder cancer, breast cancer, cervical cancer, cholangineellular carcinoma, chronic myeloid leukemia (CML), colorectal cancer, esophageal cancer, NSCLC, lymphoma, prostate cancer, renal carcinoma and small cell lung cancer (SCLC). HIG2 has been identified in renal carcinoma and SCLC. INHBB has been identified in cholangincellular carcinoma, esophageal cancer, NSCLC, renal carcinoma, SCLC and soft tissue tumor. KIF20A has been identified in bladder cancer, breast cancer, cholangincellular carcinoma, esophageal cancer, NSCLC, pancreatic cancer, prostate cancer, renal carcinoma and SCLC. KNTC2 has been identified in bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, esophageal cancer, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC and soft tissue tumor. TTK has been identified in bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, esophageal cancer, liver cancer, NSCLC, lymphoma, osteosarcoma, prostate cancer, SCLC and soft tissue tumor. URLC10 has been identified in bladder cancer, cervical cancer, cholangincellular carcinoma, esophageal cancer, gastric cancer, NSCLC, osteosarcoma, pancreatic cancer and SCLC.
    • Summary of the Invention
  • The present invention is based in part on the discovery of the applicable targets of immunotherapy. Because TAAs have often no immunogenicity, the discovery of appropriate targets is of extreme importance. As noted above, CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 have been identified as up-regulated in various cancers. More particularly, these genes were identified using gene expression profiling with a genome-wide cDNA microarray. As discussed above, expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 has been shown to be specifically up-regulated in various tumor cells, from pancreatic cancer cells to renal cell carcinomas. As described in Table 1, CDH3 expression is validly elevated in 26 out of 34 bladder cancer, 17 out of 19 cervical cancer, all of 19 cholangincellular carcinoma, 30 out of 34 colorectal cancer, 20 out of 21 endometriosis, 13 out of 20 gastric cancer, 7 out of 8 diffuse-type gastric cancer, 36 out of 37 NSCLC, all of 16 pancreatic cancer, all of 21 soft tissue tumor and all of 10 testicular tumor.
  • Table 1 further demonstrates that:
    • EPHA4 expression isvalidly elevated in 14 out of 34 bladder cancer, 8 out of 14 cervical cancer, 10 out of 25 cholangincellular carcinoma, 5 out of 15 endometriosis, 5 out of 8 diffuse-type gastric cancer, all of 5 ovarian cancer, all 14 pancreatic cancer, 20 out of 51 prostate cancer and 14 out of 23 soft tissue tumor.
    • ECT2 expression is validly elevated in 17 out of 19 bladder cancer, 5 out of 12 breast cancer, all of 14 cervical cancer, all of 13 cholangiocellular carcinoma, all of 5 CML, 7 out of 8 colorectal cancer, 12 out of 16 esophageal cancer, 6 out of 16 NSCLC, 8 out of 10 lymphoma, 1 out of 1 pancreatic cancer, 10 out of 13 prostate cancer, 3 out of 6 renal carcinoma and 12 out of 13 SCLC cancer.
    • HIG2 expression is validly elevated in 19 out of 20 renal cancer and 7 out of 9 soft tissue tumor.
    • INHBB expression is validly elevated in 10 out of 21 cholangiocellular carcinoma, all of 12 esophageal cancer, 10 out of 13 NSCLC, 22 out of 24 renal carcinoma, 8 out of 14 SCLC cancer and 45 out of 49 soft tissue tumor.
    • KIF20A expression is validly elevated in all of 31 bladder cancer, 38 out of 61 breast cancer, 10 out of 11 cholangiocellular carcinoma, 7 out of 19 esophageal cancer, 21 out of 22 NSCLC, all of 6 ovarian cancer, 17 out of 36 prostate cancer, 6 out of 11 renal carcinoma and all of 15 SCLC .
    • KNTC2 expression is validly elevated in 30 out of 32 bladder cancer, 47 out of 56 breast cancer, all of 10 cervical cancer, 16 out of 22 cholangioncellular carcinoma, 17 out of 37 CML, 3 out of 10 colorectal cancer, 11 out of 46 esophagus cancer, 15 out of 19 NSCLC, 7 out of 8 lymphoma, 20 out of 24 osteosarcoma, 3 out of 5 ovarian cancer, all of 2 pancreatic cancer, 15 out of 37 prostate cancer, 14 out of 19 renal carcinoma, all of 15 SCLC and 40 out of 59 soft tissue tumor.
    • TTK expression is validly elevated in all of 27 bladder cancer, 25 out of 30 breast cancer, 15 out of 16 cervical cancer, all of 10 cholangiocellular carcinoma, 5 out of 7 CML, 6 out of 10 colorectal cancer, 24 out of 44 esophageal cancer, 8 out of 15 liver cancer, all of 12 NSCLC, all of 6 lymphoma, 13 out of 16 osteoblastoma, 12 out of 17 prostate cancer, all of 15 SCLC and 16 out of 33 soft tissue tumor.
    • URLC10 expression is validly elevated in all of 29 bladder cancer, 15 out of 16 cervical cancer, all of 7 cholangiocellular carcinoma, 7 out of 19 esophageal cancer, all of 3 gastric cancer, 24 out of 27 NSCLC, 15 out of 19 osteosarcoma, 4 out of 5 pancreatic cancer, 33 out of 43 soft tissue tumor.
  • The present invention is based, at least in part, on the identification of specific epitope peptides of the gene products of these genes (CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10) which possess the ability to induce cytotoxic T lymphocytes (CTLs) specific to the corresponding molecules. As discussed in detail below, Peripheral Blood Mononuclear Cells (PBMC) of healthy donor were stimulated using HLA-A*2402 or HLA-A*0201 binding candidate peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10. CTL clones and/or lines were then established with specific cytotoxicity against the HLA-A24 or HLA-A2 positive target cells pulsed with each of the candidate peptides. These results demonstrate that these peptides are HLA-A24 or HLA-A2 restricted epitope peptides that can induce potent and specific immune responses against cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10.
  • Accordingly, the present invention provides methods for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10, e.g. cancer. Such methods involve the step of administering to a subject in need thereof a CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 polypeptides of the invention. Administration of such peptide(s) results in the induction of anti-tumor immunity is induced by the administration of these polypeptides. Thus, the present invention provides methods for inducing anti-tumor immunity in a subject, such methods involving the step of administering to the subject the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 polypeptides, as well as pharmaceutical compositions for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer, that include the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 polypeptides. Examples of such cancers include, but are not limited to., bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • The present invention further provides methods for preventing post-surgery recurrence of the disease mentioned above.
    Regarding the specific aims and objectives recited above, it will be understood by those skilled in the art that one or more aspects of this invention can meet certain objectives, while one or more other aspects can meet certain other objectives. Each objective may not apply equally, in all its respects, to every aspect of this invention. As such, the objects herein can be viewed in the alternative with respect to any one aspect of this invention.
  • Additional objects and features of the invention will become more fully apparent when the following detailed description is read in conjunction with the accompanying figures and examples. However, it is to be understood that both the foregoing summary of the invention and the following detailed description are of preferred embodiments, and not restrictive of the invention or other alternate embodiments of the invention. In particular, while the invention is described herein with reference to a number of specific embodiments, it will be appreciated that the description is illustrative of the invention and is not constructed as limiting of the invention. Various modifications and applications may occur to those who are skilled in the art, without departing from the spirit and the scope of the invention, as described by the appended claims. Likewise, other objects, features, benefits and advantages of the present invention will be apparent from this summary and certain embodiments described below, and will be readily apparent to those skilled in the art. Such objects, features, benefits and advantages will be apparent from the above in conjunction with the accompanying examples, data, figures and all reasonable inferences to be drawn therefrom, alone or with consideration of the references incorporated herein.
    • Brief Description of the Drawings
  • Various aspects and applications of the present invention will become apparent to the skilled artisan upon consideration of the brief description of the figures and the detailed description of the present invention and its preferred embodiments which follows:
    • [fig.1-1]Figure 1 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that CDH3-A24-10-332 (SEQ ID NO: 34), CDH3-A24-10-470 (SEQ ID NO: 358), CDH3-A24-9-513 (SEQ ID NO: 19), CDH3-A24-9-406 (SEQ ID NO: 22), CDH3-A24-10-807 (SEQ ID NO: 30) and CDH3-A24-10-655 (SEQ ID NO: 344) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA-A*2402. "b" depicts the CTL-inducing ability of CDH3-A24-10-332 (SEQ ID NO: 34). CDH3-A24-10-332 (SEQ ID NO: 34) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #4 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "c" depicts the CTL-inducing ability of CDH3-A24-10-470 (SEQ ID NO: 358). CDH3-A24-10-470 (SEQ ID NO: 358) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #4 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "d" depicts the CTL-inducing ability of CDH3-A24-9-513 (SEQ ID NO: 19). CDH3-A24-9-513 (SEQ ID NO: 19) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay. The well #6 shown in boxed wells in left panel demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL line that was established from the positive well #5 shown in boxed wells in middle panel, demonstrated the specific response against the target cells pulsed with the epitope peptide. "e" depicts the CTL-inducing ability of CDH3-A24-9-406 (SEQ ID NO: 22). CDH3-A24-9-406 (SEQ ID NO: 22) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #2 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide.
    • [fig.1-2]Figure 1 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that CDH3-A24-10-332 (SEQ ID NO: 34), CDH3-A24-10-470 (SEQ ID NO: 358), CDH3-A24-9-513 (SEQ ID NO: 19), CDH3-A24-9-406 (SEQ ID NO: 22), CDH3-A24-10-807 (SEQ ID NO: 30) and CDH3-A24-10-655 (SEQ ID NO: 344) show potent IFN-gamma production. "f" depicts the CTL-inducing ability of CDH3-A24-10-807 (SEQ ID NO: 30). CDH3-A24-10-807 (SEQ ID NO: 30) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and the clone were established from the positive well #5 shown in boxed wells. The established CTL clone raised against the peptide demonstrated the specific CTL activity against COS7 transfected both full length of CDH3 gene and HLA-A24 molecule (lower right graph). On the other hand, COS7 transfected full length of CDH3 but not HLA-A24 and COS7 transfected HLA-A24 but not full length of CDH3 were prepared for the negative control. The CTL clone showed high specific CTL activity against COS7 that transfected both CDH3 and HLA-A24. "g" depicts the CTL-inducing ability of CDH3-A24-10-655 (SEQ ID NO: 344). CDH3-A24-10-655 (SEQ ID NO: 344) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and the clone were established from the positive well #1 shown in boxed wells. The established CTL clone raised against the peptide demonstrated the specific CTL activity against COS7 transfected both full length of CDH3 gene and HLA-A24 molecule (lower right graph). On the other hand, COS7 transfected full length of CDH3 but not HLA-A24 and COS7 transfected HLA-A24 but not full length of CDH3 were prepared for the negative control. The CTL clone showed high specific CTL activity against COS7 that transfected both CDH3 and HLA-A24.
    • [fig.2]Figure 2 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that Epha4-A24-9-453 (SEQ ID NO: 41), Epha4-A24-9-5 (SEQ ID NO: 44), Epha4-A24-9-420 (SEQ ID NO: 48), Epha4-A24-9-869 (SEQ ID NO: 46), Epha4-A24-10-24 (SEQ ID NO: 78) Epha4-A02-9-501 (SEQ ID NO: 376) and Epha4-A02-9-165 (SEQ ID NO: 379) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of Epha4-A24-9-453 (SEQ ID NO: 41). Epha4-A24-9-453 (SEQ ID NO: 41) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #3 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "c" depicts the CTL-inducing ability of Epha4-A24-9-5 (SEQ ID NO: 44). Epha4-A24-9-5 (SEQ ID NO: 44) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #2 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "d" depicts the CTL-inducing ability of Epha4-A24-9-420 (SEQ ID NO: 48). Epha4-A24-9-420 (SEQ ID NO: 48) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay. The well #6 shown in boxed wells in upper panel demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover CTL line that was established from the positive well #6 shown in boxed wells in middle panel, demonstrated the specific response against the target cells pulsed with the epitope peptide. "e" depicts the CTL-inducing ability of Epha4-A24-9-869 (SEQ ID NO: 46). Epha4-A24-9-869 (SEQ ID NO: 46) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #5 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "f" depicts the CTL-inducing ability of Epha4-A24-10-24 (SEQ ID NO: 78). Epha4-A24-10-24 (SEQ ID NO: 78) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line that was established from the positive well #4 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "g" depicts the CTL-inducing ability of Epha4-A02-9-501 (SEQ ID NO: 376). Epha4-A02-9-501 (SEQ ID NO: 376) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone was established from the positive well #8 shown in boxed wells. Cytotoxic activity of the established CTL line against the target cells pulsed with the peptide was measured by Cr-release assay (CRA) (lower graph), and the CTL line had very potent specific cytotoxic activity against the target cells pulsed with the peptides. "h" depicts the CTL-inducing ability of Epha4-A02-9-165 (SEQ ID NO: 379). Epha4-A02-9-165 (SEQ ID NO: 379) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line was established from the positive well #3 shown in boxed wells. Cytotoxic activity of the established CTL line against target cells pulsed with peptide was measured by Cr-release assay (CRA) (right graph), and the CTL line had very potent specific cytotoxic activity against the target cells pulsed with the peptides.
    • [fig.3]Figure 3 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that ECT2-A24-9-515 (SEQ ID NO: 80), ECT2-A24-10-40 (SEQ ID NO: 100) and ECT2-A24-10-101 (SEQ ID NO: 101) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of ECT2-A24-9-515 (SEQ ID NO: 80). ECT2-A24-9-515 (SEQ ID NO: 80) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay. The well #5 and #7 shown in boxed wells in left panel demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL line that was established from the positive well #7 shown in boxed wells in second panel, demonstrated the specific response against the target cells pulsed with the epitope peptide. Cytotoxic activity of the CTL line against cancer cell line, TE6 endogenously expressing ECT2 and HLA-A24 was measured by Cr-release assay (CRA), and the CTL clone had very potent cytotoxic activity against TE6. On the other hand, the effector cells did not demonstrate the cytotoxic activity of the CTL line against cancer cell line, TE5 expressing only ECT2 was not detected. "c" depicts the CTL-inducing ability of ECT2-A24-10-40 (SEQ ID NO: 100). ECT2-A24-10-40 (SEQ ID NO: 100) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and the clone were established from the positive well #2 shown in boxed wells. The established CTL clone raised against the peptide demonstrated specific CTL activity against COS7 transfected both full length of ECT2 gene and HLA-A24 molecule. On the other hand, COS7 transfected full length of ECT2 but not HLA-A24, COS7 transfected HLA-A24 and URLC10 gene as a substitute for full length of ECT2 and COS7 transfected HLA-A24 and pulsed with ECT2-10-101 were prepared for the negative control. The CTL clone showed high specific CTL activity against COS7 that transfected both ECT2 and HLA-A24. "d" depicts the CTL-inducing ability of ECT2-A24-10-101 (SEQ ID NO: 101). ECT2-A24-10-101 (SEQ ID NO: 101) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line were established from the positive well #1 shown in boxed wells. The established CTL line raised against the peptide demonstrated specific CTL activity against COS7 transfected both full length of ECT2 gene and HLA-A24 molecule. COS7 transfected full length of ECT2 but not HLA-A24, COS7 transfected HLA-A24 and URLC10 gene as substitute for full length of ECT2 and COS7 transfected HLA-A24 and pulsed with ECT2-10-40 were prepared for the negative control. The CTL clone showed high specific CTL activity against COS7 that transfected both ECT2 and HLA-A24.
    • [fig.4-1]Figure 4 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that HIG2-A24-9-19 (SEQ ID NO: 110), HIG2-A24-9-22 (SEQ ID NO: 111), HIG2-A24-9-8 (SEQ ID NO: 387), HIG2-A24-10-7 (SEQ ID NO: 112), HIG2-A24-10-18 (SEQ ID NO: 394), HIG2-A02-9-15 (SEQ ID NO: 116), HIG2-A02-9-4 (SEQ ID NO: 117) and HIG2-A02-10-8 (SEQ ID NO: 121) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of HIG2-A24-9-19 (SEQ ID NO: 110). HIG2-A24-9-19 (SEQ ID NO: 110) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #6 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "c" depicts the CTL-inducing ability of HIG2-A24-9-22 (SEQ ID NO: 111). HIG2-A24-9-22 (SEQ ID NO: 111) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone, that was established from the positive well #7 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "d" depicts the CTL-inducing ability of HIG2-A24-9-8 (SEQ ID NO: 387). HIG2-A24-9-8 (SEQ ID NO: 387) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone, that were established from the positive well #5 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "e" depicts the CTL-inducing ability of HIG2-A02-9-8 (SEQ ID NO: 114). HIG2-A02-9-8 (SEQ ID NO: 114) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line was established from the positive well #10 shown in boxed wells. The established CTL line raised against the peptide demonstrate specific CTL activity against 293T transfected both full length of HIG2 gene and HLA-A02 molecule. 293T transfected full length of HIG2 but not HLA-A02, 293Ts transfected HLA-A02 and FoxP3 gene as substitute dor full length of HIG2 and 293Ts transfected HLA-A02 and pulsed with HIG2-9-15 were prepared for the negative control. The CTL line showed high specific CTL activity against 293T that transfected both HIG2 and HLA-A02.
    • [fig.4-2]Figure 4 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that HIG2-A24-9-19 (SEQ ID NO: 110), HIG2-A24-9-22 (SEQ ID NO: 111), HIG2-A24-9-8 (SEQ ID NO: 387), HIG2-A24-10-7 (SEQ ID NO: 112), HIG2-A24-10-18 (SEQ ID NO: 394), HIG2-A02-9-15 (SEQ ID NO: 116), HIG2-A02-9-4 (SEQ ID NO.1 117) and HIG2-A02-10-8 (SEQ ID NO: 121) show potent IFN-gamma production. "f" depicts the CTL-inducing ability of HIG2-A24-10-7 (SEQ ID NO: 112). HIG2-A24-10-7 (SEQ ID NO: 112) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL lines or clone, that were established from the positive well #1 and #7 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "g" depicts the CTL-inducing ability of HIG2-A24-10-18 (SEQ ID NO: 394). HIG2-A24-10-18 (SEQ ID NO: 394) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone, that were established from the positive well #7 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "h" depicts the CTL-inducing ability of HIG2-A02-9-15 (SEQ ID NO: 116). HIG2-A02-9-15 (SEQ ID NO: 116) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line was established from the positive well #10 shown in boxed wells. The established CTL line raised against the peptide demonstrated specific CTL activity against COS7 transfected both full length of HIG2 gene and HLA-A02 molecule. COS7 transfected full length of HIG2 but not HLA-A02 and COS7s transfected HLA-A02 and pulsed with HIG2-9-8 peputide were prepared for the negative control. The CTL line showed high specific CTL activity against COS7 that transfected both HIG2 and HLA-A02.
    • [fig.4-3]Figure 4 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that HIG2-A24-9-19 (SEQ ID NO: 110), HIG2-A24-9-22 (SEQ ID NO: 111), HIG2-A24-9-8 (SEQ ID NO: 387), HIG2-A24-10-7 (SEQ ID NO: 112), HIG2-A24-10-18 (SEQ ID NO: 394), HIG2-A02-9-15 (SEQ ID NO: 116), HIG2-A02-9-4 (SEQ ID NO: 117) and HIG2-A02-10-8 (SEQ ID NO: 121) show potent IFN-gamma production. "i" depicts the CTL-inducing ability of HIG2-A02-9-4 (SEQ ID NO: 117). HIG2-A02-9-4 (SEQ ID NO: 117) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone were established from the positive well #10 shown in boxed wells. The established CTL line raised against the peptide demonstrated specific CTL activity against COS7 transfected both full length of HIG2 gene and HLA-A02 molecule (middle graph). Also, COS7 transfected full length of HIG2 but not HLA-A02, COS7s transfected HLA-A02 and TTK gene as substitute for full length of HIG2 and COS7s transfected HLA-A02 and pulsed with HIG2-9-8 were prepared for the negative control. Cytotoxic activity of the CTL clone against 293T, transfected both full length of HIG2 gene and HLA-A02 molecule, and cancer cell line, Caki-1 endogenously expressing HIG2 and HLA-A02 was measured by Cr-release assay (CRA) (lower graphs), and the CTL clone had very potent cytotoxic activity against the transfectant with both of HiG2 gene and HLA-A02, and Caki-1. On the other hand, the effector cells did not demonstrate the cytotoxic activity of the CTL line against 293T, transfected only HIG2 or only HLA-A02, and cancer cell line, A498 expressing only HIG2 was not detected, "j" depicts the CTL-inducing ability of HIG2-A02-10-8 (SEQ ID NO: 121). HIG2-A02-10-8 (SEQ ID NO: 121) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #9 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide.
    • [fig.5-1]Figure 5 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that INHBB-A24-9-180 (SEQ ID NO: 395), INHBB-A24-10-180 (SEQ ID NO: 133), INHBB-A24-10-305 (SEQ ID NO: 135), INHBB-A24-10-7 (SEQ ID NO: 137) and INHBB-A24-10-212 (SEQ ID NO: 426) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of INHBB-A24-9-180 (SEQ ID NO: 395). INHBB-A24-9-180 (SEQ ID NO: 395) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone was established from the positive well #7 shown in boxed wells. Cytotoxic activity of the established CTL clone against tumor cells, Miapaca2 expressing both of INHBB and HLA-A02 was measured by Cr-release assay (CRA), and the effector cells showed high specific cytotoxic activity against Miapaca2. On the other hand, it did not show significant specific cytotoxic activity against Caki-1 expressing INHBB but not HLA-A02. "c" depicts the CTL-inducing ability of INHBB-A24-10-180 (SEQ ID NO: 133). INHBB-A24-10-180 (SEQ ID NO: 133) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line was established from the positive well #3 shown in boxed wells. The established CTL line raised against the peptide demonstrated high specific CTL activity against 293T transfected both of full length of INHBB gene and HLA-A24 molecule. Also, 293T transfected full length of INHBB but not HLA-A24 and 293Ts transfected HLA-A24 and pulsed with INHBB-10-305 peptide were prepared for the negative control.
    • [fig.5-2]Figure 5 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that INHBB-A24-9-180 (SEQ ID NO: 395), INHBB-A24-10-180 (SEQ ID NO: 133), INHBB-A24-14-305 (SEQ ID NO: 135), INHBB-A24-10-7 (SEQ ID NO: 137) and INHBB-A24-10-212 (SEQ ID NO: 426) show potent IFN-gamma production. "d" depicts the CTL-inducing ability of INHBB-A24-10-305 (SEQ ID NO: 135). INHBB-A24-10-305 (SEQ ID NO: 135) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone were established from the positive well #2 shown in boxed wells. The established CTL clone raised against the peptide demonstrated high specific CTL activity against 293T transfected both full length of INHBB gene and HLA-A24 molecule. Also, 293T transfected full length of INHBB but HLA-A24 and 293Ts transfected HLA-A24 and pulsed with INHBB-10-180 peptide were prepared for the negative control. "e" depicts the CTL-inducing ability of INHBB-A24-10-7 (SEQ ID NO: 137)). INHBB-A24-10-7 (SEQ ID NO: 137) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL lines were established from the positive well #8 shown in boxed wells in upper panel and #2 shown in boxed wells in lower panel. The CTL line from #8 well demonstrated specific CTL activity against 293T transfected both full length of INHBB gene and HLA-A24 molecule. Also, 293T transfected full length of INHBB but not HLA-A24 and 293Ts transfected HLA-A24 and pulsed with INHBB-10-40 peptide were prepared for the negative control. "f" depicts the CTL-inducing ability of INHBB-A24-10-212 (SEQ ID NO: 426). INHBB-A24-10-212 (SEQ ID NO: 426) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #1 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide.
    • [fig.6-1]Figure 6 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that KIF20A-A24-10-304 (SEQ ID NO: 186), KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-66 (SEQ ID NO: 194) and KIF20A-A24-9-305 (SEQ ID NO: 174) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of KIF20A-A24-10-304 (SEQ ID NO: 186). KIF20A-A24-10-304 (SEQ ID NO: 186) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay. The well #5 shown in boxed wells in lower right panel demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL line and clone, that were established from the positive well #5 shown in boxed wells in upper left panel, also demonstrated the specific response against the target cells pulsed with the epitope peptide. The established CTL clone raised against the peptide demonstrated specific CTL activity against 24-LCL transfected full length of KIF20A gene. Also, A24-LCL transfected mock vector was prepared for the negative control. Cytotoxic activity of the CTL clone against tumor cells, Miapaca2 expressing both of KIF20A and HLA-A24 was measured by Cr-release assay (CRA), and the CTL clone had very potent specific cytotoxic activity against Miapaca2 (lower right graph). On the other hand, it did not show significant specific cytotoxic activity against PK59 expressing KIF20A but not HLA-A24. "c" depicts the CTL-inducing ability of KIF20A-A24-9-383 (SEQ ID NO: 178). KIF20A-A24-9-383 (SEQ ID NO: 178) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay. The well # 3 and 4 shown in boxed wells in right panel demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL line, that was established from the positive well #3 shown in boxed wells in left panel, also demonstrated the specific response against the target cells pulsed with the epitope peptide. The established CTL line demonstrated high specific CTL activity against COS7 transfected both full length of KIF20A gene and HLA-A24 molecule. Also, COS7 transfected full length of KIF20A but not HLA-A24 and COS7s transfected HLA-A24 and pulsed with KIF20A-3-621 peptide were prepared for the negative control.
    • [fig.6-2]Figure 6 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that KIF20A-A24-10-304 (SEQ ID NO: 186), KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-66 (SEQ ID NO: 194) and KIF20A-A24-9-305 (SEQ ID NO: 174) show potent IFN-gamma production. "d" depicts the CTL-inducing ability of KIF20A-A24-10-66 (SEQ ID NO: 194). KIF20A-A24-10-66 (SEQ ID NO: 194) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL lines, that were established from the positive well #6 shown in boxed wells in upper left panel and #3 shown in boxed wells in lower middle panel demonstrated the specific response against the target cells pulsed with the epitope peptide, Moreover, CTL clone selected from CTL line from #6 well by limiting dilution demonstrated specific CTL activity against the target cells. The established CTL clone showed specific CTL activity against COS7 transfected both full length of KIF20A gene and HLA-A24 molecule. Also, COS7 transfected full length of KIF20A but not HLA-A24, COS7s transfected HLA-A24 and URLC10 gene as substitute for full length of KIF20A and COS7 transfected HLA-A24 and pulsed with KIF20A-10-308 peptide were prepared for the negative control. "e" depicts the CTL-inducing ability of KIF20A-A24-9-305 (SEQ ID NO: 174). KIF20A-A24-9-305 (SEQ ID NO: 174) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL lines, that were established from the positive well #2 shown in boxed wells in upper left panel and #6 shown in boxed wells in lower middle panel, demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL clone selected from CTL line from #2 well by limiting dilution demonstrated specific CTL activity against the target cells. Cytotoxic activity of the CTL clone against tumor cells, MIapaca2 expressing both of KIF20A and HLA-A24 was measured by Cr-release assay (CRA), and the CTL clone had very potent cytotoxic activity against MIapaca2. On the other hand, it did not show significant specific cytotoxic activity against PK59 expressing KIF20A but not HLA-A24.
    • [fig.7-1]Figure 7 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that KNTC2-A24-9-309 (SEQ ID NO: 196), KNTC2-A24-9-124 (SEQ ID NO: 202), KIF20A-A24-9-154 (SEQ ID NO: 210) KNTC2-A24-9-150 (SEQ ID NO: 213), KNTC2-A24-10-452 (SEQ ID NO: 214), KNTC2-A24-10-227 (SEQ ID NO: 217) and KNTC2-A24-10-273 (SEQ ID NO: 223) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of KNTC2-A24-9-309 (SEQ ID NO: 196). KNTC2-A24-9-309 (SEQ ID NO: I96) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #8 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitome peptide.. "c" depicts the CTL-inducing ability of KNTC2-A24-9-124 (SEQ ID NO: 202). KNTC2-A24-9-124 (SEQ ID NO: 202) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #5 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "d" depicts the CTL-inducing ability of KIF20A-A24-9-154 (SEQ ID NO: 210). KIF20A-A24-9-154 (SEQ ID NO: 210) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clone, that were established from the positive well #5 shown in boxed wells demonstrated the specific response against the target cells pulsed with the epitope peptide. "e" depicts the CTL-inducing ability of KNTC2-A24-9-150 (SEQ ID NO: 213). KNTC2-A24-9-150 (SEQ ID NO: 213) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #7 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide.
    • [fig.7-2]Figure 7 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that KNTC2-A24-9-309 (SEQ ID NO: 196), KNTC2-A24-9-124 (SEQ ID NO: 202), KIF20A-A24-9-154 (SEQ ID NO: 210) KNTC2-A24-9-150 (SEQ ID NO: 213), KNTC2-A24-10-452 (SEQ ID NO: 214), KNTC2-A24-10-227 (SEQ ID NO: 217) and KNTC2-A24-10-273 (SEQ ID NO: 223) show potent IFN-gamma production. "f" depicts the CTL-inducing ability of KNTC2-A24-10-452 (SEQ ID NO: 214). KNTC2-A24-10-452 (SEQ ID NO: 214) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL lines and clone, that were established from the positive well #4 shown in boxed wells in upper left panel and #5 shown in boxed wells in middle panel, demonstrated the specific response against the target cells pulsed with the epitope peptide. Moreover, CTL clone selected from CTL line from #5 well by limiting dilution demonstrated specific CTL activity against the target cells. The established CTL line from #4 well showed specific CTL activity against HEK293 transfected both full length of KNTC2 gene and HLA-A24 molecule. Also, HEK293 transfected full length of KNTC2 but not HLA-A24, HEK293 transfected HLA-A24 but full length of KNTC2 and HEK293 transfected HLA-A24 pulsed with KNTC-9-309 peptide were prepared for the negative control. "g" depicts the CTL-inducing ability of KNTC2-A24-10-227 (SEQ ID NO: 217). KNTC2-A24-10-227 (SEQ ID NO: 217) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #1 shown in boxed well s, demonstrated the specific response against the target cells pulsed with the epitope peptide. "h" depicts the CTL-inducing ability of KNTC2-A24-10-273 (SEQ ID NO: 223). KNTC2-A24-10-273 (SEQ ID NO: 223) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #8 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide.
    • [fig.8-1]Figure 8 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that TTK-A02-9-462 (SEQ ID NO: 227), TTK-A02-9-719 (SEQ ID NO: 233), TTK-A02-9-547 (SEQ ID NO: 228) and TTK-A02-10-462 (SEQ ID NO: 254), show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of TTK-A02-9-462 (SEQ ID NO: 227). TTK-A02-9-462 (SEQ ID NO: 227) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and two clones, that were established from the positive well #4 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. The established CTL clone showed high specific CTL activity against COS7 transfected both full length of TTK gene and HLA-A02 molecule. Also, COS7 transfected full length of TTK but not HLA-A02, COS7s transfected HLA-A02 but not full length of TTK and COS7s transfected HLA-A02 pulsed with TTK-9-547 peptide were prepared for the negative control. "c" depicts the CTL-inducing ability of TTK-A02-9-719 (SEQ ID NO: 233). TTK-A02-9-719 (SEQ ID NO: 233) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clones were established from the positive well #1 shown in boxed wells. The established CTL line showed high specific CTL activity against COS7 transfected both full length of TTK gene and HLA-A02 molecule. Also, COS7 transfected full length of TTK but not HLA-A02 and COS7s transfected HLA-A02 and HIG2 gene as substitute for full length of TTK were prepared for the negative control [fig.8-2]Figure 8 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that TTK-A02-9-462 (SEQ ID NO: 227), TTK-A02-9-719 (SEQ ID NO: 233), TTK-A02-9-547 (SEQ ID NO: 228) and TTK-A02-10-462 (SEQ ID NO: 254), show potent IFN-gamma production. "d" depicts the CTL-inducing ability of TTK-A02-9-547 (SEQ ID NO: 228). TTK-A02-9-547 (SEQ ID NO: 228) demonstrated potent IFN-gamma production as compared to the control by IFN-gainma ELISPOT assay, and CTL line and clones were established from the positive well #2 shown in boxed wells. The established CTL line showed specific CTL activity against COS7 transfected both full length of TTK gene and HLA-A02 molecule. Also, COS7 transfected full length of TTK but not HLA-A02, COS7s transfected HLA-A02 but not full length of TTK and COS7s transfected HLA-A02 and pulsed with TTK-10-462 were prepared for the negative control.
    • [fig.8-3]Figure 8 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that TTK-A02-9-462 (SEQ ID NO: 227), TTK-A02-9-719 (SEQ ID NO: 233), TTK-A02-9-547 (SEQ ID NO: 228) and TTK-A02-10-462 (SEQ ID NO: 254), show potent IFN-gamma production. "e" depicts the CTL-inducing ability of TTK-A02-10-462 (SEQ ID NO: 254). TTK-A02-10-462 (SEQ ID NO: 254) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and three clones were established from the positive well #8 shown in boxed wells. The established CTL clone showed specific CTL activity against COS7 transfected both full length of TTK gene and HLA-A02 molecule. Also, COS7 transfected full length of TTK but not HLA-A02, COS7s transfected HLA-A02 but not full length of TTK and COS7s transfected HLA-A02 and pulsed with TTK-9-547 peptide were prepared for the negative control.
    • [fig.9-1]Figure 9 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that URLC10-A02-9-206 (SEQ ID NO: 271), URLC10-A02-9-212 (SEQ ID NO: 272) and URLC10-A02-10-211 (SEQ ID NO: 288) show potent IFN-gamma production. "a" depicts the example of negative peptides which could not be detected CTL-inducing ability despite possible binding activity with HLA. "b" depicts the CTL-inducing ability of URLC10-A02-9-206 (SEQ ID NO: 271). URLC10-A02-9-206 (SEQ ID NO: 271) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #7 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "c" depicts the CTL-inducing ability of URLC10-A02-9-212 (SEQ ID NO: 272). URLC10-A02-9-212 (SEQ ID NO: 272) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line, that was established from the positive well #3 shown in boxed wells, demonstrated the specific response against the target cells pulsed with the epitope peptide. "d" depicts the CTL-inducing ability of URLC10-A02-10-211 (SEQ ID NO: 288). URLC10-A02-10-211 (SEQ ID NO: 288) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and CTL line and clones, that were established from the positive well #5 shown in boxed wells.
    • [fig.9-2]Figure 9 depicts the results of the screening of epitope peptides, which, in turn, demonstrate that URLC10-A02-9-206 (SEQ ID NO: 271), URLC10-A02-9-212 (SEQ ID NO: 272) and URLC10-A02-10-211 (SEQ ID NO: 288) show potent IFN-gamma production. " Continuation of d" The established CTL clone showed high specific CTL activity against COS7, Hek293 and 293T which were transfected both full length of URLC10 gene and HLA-A02 molecule. Also, COS7, Hek293 or 293T which were transfected full length of URLC10 but not HLA-A02 and COS7s, Hek293s or 293Ts, which were transfected HLA-A02 and pulsed with URLC10-10-64, were prepared for the negative control. In this drawings, "+" means the peptide pulsed target, "-" means the no peptide pulsed target, "R" means Responder, "S" means Stimulator, "E" means Effector, and "T" means Target.
    Detailed Description of the Invention
  • The words "a", "an", and "the" as used herein mean "at least one" unless otherwise specifically indicated.
  • Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
  • The present invention is based in part on the discovery of applicable targets of immunotherapy. Identification of new TAAs, particularly those that induce potent and specific anti-tumor immune responses, warrants further development of the clinical application of the peptide vaccination strategy in various types of cancer (Boon T et al., (1996) J Exp Med 183: 725-9.; van der Bruggen P et al., (1991) Science 254: 1643-7.; Brichard V et al., (1993) J Exp Med 178: 489-95.; Kawakami Y et al., (1994) J Exp Med 180: 347-52.; Shichijo S et al., (1998) J Exp Med 187:277-88.; Chen YT et al., (1997) Proc.Natl.Acd. Sci.USA, 94: 1914-8.; Harris CC, (1996) J Natl Cancer Inst 88:1442-55.; Butterfield LH et al., (1999) Cancer Res 59:3134-42.; Vissers JL et al., (1999) Cancer Res 59: 5554-9.; van der Burg SH et al., (1996) J. Immunol 156:3308-14.; Tanaka F et al.., (1997) Cancer Res 57:4465-8.; Fujie T et al., (1999) Int J Cancer 80:169-72.; Kikuchi M et al., (1999) Int J Cancer 81 : 459-66.; Oiso M et al., (1999) Int J Cancer 81:387-94.). Because TAAs have often no immunogenicity, discovery of fitting targets is extremely important issue.
  • As noted above,
    • CDH3 (GenBank Accession No. M_001793; SEQ ID Nos.1, 2),
    • EPHA.4 (GenBank Accession No. L36645; SEQ ID Nos.3, 4),
    • ECT2 (GenBank Accession No. AY376439; SEQ ID Nos.5, 6),
    • HIG2 (GenBank Accession No. NM_013332; SEQ ID Nos.7, 8)
    • INHBB (GenBank Accession No. NM_002193; SEQ ID Nos.9, 10),
    • KIF20A (GenBank Accession No. NM_005733; SEQ ID Nos.11, 12),
    • KNTC2 (GenBank Accession No. AF017790; SEQ ID Nos.13, 14),
    • TTK (GenBank Accession No. NM_003318; SEQ ID Nos.15, 16) and
    • URLC10 (GenBank Accession No. NM_017527; SEQ ID Nos.17, 18) were previously identified as over-expressed in various cancers using cDNA microarray technologies.
  • In the present invention, peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 are shown to be TAA epitopes restricted by HLA-A24 and HLA-A2, an HLA allele commonly found in the Japanese and Caucasian populations. Specifically, using their binding affinities to HLA-A24 or HLA-A2, candidates of HLA-A24 or HLA-A2 binding peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 were identified. After the in vitro stimulation of T-cells by dendritic cells (DCs) loaded with these peptides, CTLs were successfully established using the following peptides.
    • CDH3-A24-9-513 (SEQ ID NO: 19),
    • CDH3-A24-9-406 (SEQ ID NO: 22),
    • CDH3-A24-10-807 (SEQ ID NO: 30),
    • CDH3-A24-10-332 (SEQ ID NO: 34),
    • CDH3-A24-10-655 (SEQ ID NO: 344),
    • CDH3-A24-10-470 (SEQ ID NO: 358),
    • EphA4-A24-9-453 (SEQ ID NO: 41),
    • EphA4-A24-9-5 (SEQ ID NO: 44),
    • EphA4-A24-9-869 (SEQ ID NO: 46),
    • EphA4-A24-9-420 (SEQ ID NO: 48),
    • EphA4-A24-10-24 (SEQ ID NO: 78),
    • EphA4-A02-9-501 (SEQ ID NO: 376),
    • EphA4-A02-9-165 (SEQ ID NO: 379),
    • ECT2-A24-9-515 (SEQ ID NO: 80),
    • ECT2-A24-10-40 (SEQ ID NO: 100).
    • ECT2-A24-10-101 (SEQ ID NO: 101),
    • HIG2-A24-9-19 (SEQ ID NO: 110),
    • HIG2-A24-9-22 (SEQ ID NO: 111),
    • HIG2-A24-9-8 (SEQ ID NO: 387),
    • HIG2-A24-10-7 (SEQ ID NO: 112),
    • HIG2-A24-10-18 (SEQ ID NO: 394),
    • HIG2-A02-9-8 (SEQ ID NO: 114),
    • HIG2-A02-9-15 (SEQ ID NO: 116),
    • HIG2-A02-9-4 (SEQ ID NO: 117),
    • HIG2-A02-10-8 (SEQ ID NO: 121),
    • INHBB-A24-9-180 (SEQ ID NO: 395),
    • INHBB-A24-10-180 (SEQ ID NO: 133),
    • INHBB-A24-10-305 (SEQ ID NO: 135),
    • INHBB-A24-10-7 (SEQ ID NO: 137),
    • INHBB-A24-10-212 (SEQ ID NO: 426),
    • KIF20A-A24-9-305 (SEQ ID NO: 174),
    • KIF20A-A24-9-383 (SEQ ID NO: 178),
    • KIF20A-A24-10-304 (SEQ ID NO: 186),
    • KIF20A-A24-10-66 (SEQ ID NO: 194),
    • KNTC2-A24-9-309 (SEQ ID NO: 196),
    • KNTC2-A24-9-124 (SEQ ID NO: 202),
    • KNTC2-A24-9-154 (SEQ ID NO: 210),
    • KNTC2-A24-9-150 (SEQ ID NO: 213),
    • KNTC2-A24-10-452 (SEQ ID NO: 214),
    • KNTC2-A24-10-227 (SEQ ID NO: 217),
    • KNTC2-A24-10-273 (SEQ ID NO: 223),
    • TTK-A02-9-462 (SEQ ID NO: 227),
    • TTK-A02-9-547 (SEQ ID NO: 228),
    • TTK-A02-9-719 (SEQ ID NO: 233),
    • TTK-A02-10-462 (SEQ ID NO: 254),
    • URLC-A02-9-206 (SEQ ID NO: 271),
    • URLC-A02-9-212 (SEQ ID NO: 272) and
    • URLC-A02-10-211 (SEQ ID NO: 288)
  • These peptides are epitope peptides of each TAA restricted by HLA-A24 or HLA-A2. Since these antigens are over-expressed in most cancers and are associated with tumor cell proliferation, they find utility as immunotherapeutic targets against cancers. Exemplary cancers include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • Accordingly, the present invention further provides methods of treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers in a subject, such methods including the steps of administering to the subject an immunogenic peptide of less than about 40 amino acids, often less than about 20 amino acids, usually less than about 15 amino acids and having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288.
  • Alternatively, the immunogenic peptide may have an amino acid sequence as set forth in SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 in which 1, 2, or several (e.g., up to 5) amino acids are substituted, deleted or added, provided the resulting variant peptide retains the immunogenic activity (i.e., the ability to induce CTLs specific to cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers).
  • The number of residues to be substituted, deleted, or added is generally 5 amino acids or less, preferably 4 amino acids or less, more preferably 3 amino acids or less, even more preferably one or two amino acids. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. Furthermore the present invention provides methods for preventing post-surgery recurrence of these diseases mentioned above.
  • Variant peptides (i.e., peptides having an amino acid sequence modified by substituting, deleting, or adding one, two or several amino acid residues to an original amino acid sequence) are known to retain the original biological activity (Mark DF et al., (1984) Proc Natl Acad Sci USA 81: 5662-6.; Zoller MJ and Smith M, (1982) Nucleic Acids Res 10:6487-500.; Dalbadie-McFarland G et al., (1982) Proc Natl Acad Sci USA 79: 6409-13.). In the context of the present invention, it is preferable that the amino acid modification results in conservation of the properties of the original amino acid side-chain (a process known as conservative amino acid substitution). Examples of properties of amino acid side chains include hydrophobic amino acids (A, I, L, M, F, P, W, Y, V), hydrophilic amino acids (R, D, N, C, E, Q, G, H, K, S, T), and side chains having the following functional groups or characteristics in common: an aliphatic side-chain (G, A, V, L, I, P); a hydroxyl group containing side-chain (S, T, Y); a sulfur atom containing side-chain (C, M); a carboxylic acid and amide containing side-chain (D, N, E, Q); a base containing side-chain (R, K, H); and an aromatic containing side-chain (H, F, Y, W). Note, the parenthetic letters indicate the one-letter codes of amino acids.
  • In preferred embodiments, the immunogenic peptide is a nonapeptide (9-mer) or a decapeptide (10-mer).
    The present invention further provides a method of inducing anti-tumor immunity for a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, in a subject, such a method including the steps of administering to the subject an immunogenic peptide of the present invention, namely one having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288, or a variant thereof (i.e., including 1, 2, or several (e.g., up to 5) amino acid substitutions, deletions, or additions) to the subject in need thereof. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • In the context of the present invention, the subject is preferably a mammal. Exemplary mammals include, but are not limited to, e.g., a human, non-human primate, mouse, rat, dog, cat, horse, or cow.
    In the present invention, the peptide can be administered to a subject via an in vivo or ex vivo protocol. Furthermore, the present invention also provides use of nonapeptide or decapeptide selected from peptides having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 (and variants thereof) for manufacturing an immunogenic composition for treating or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, K11TTC2, TTK and/or URLC10, e.g. cancers. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • Homology analyses of the following peptides demonstrate that they do not have significant homology with the peptides derived from any known human gene products.
    • CDH3-A24-9-513 (SEQ ID NO: 19),
    • CDH3-A24-9-406 (SEQ ID NO: 22),
    • CDH3-A24-10-807 (SEQ ID NO: 30),
    • CDH3-A24-10-332 (SEQ ID NO: 34),
    • CDH3-A24-10-655 (SEQ ID NO: 344),
    • CDH3-A24-10-470 (SEQ ID NO: 358),
    • EphA4-A24-9-453 (SEQ ID NO: 41),
    • EphA4-A24-9-5 (SEQ ID NO: 44),
    • EphA4-A24-9-869 (SEQ ID NO: 46),
    • EphA4-A24-.9-420 (SEQ ID NO: 48),
    • EphA4-A24-10-24 (SEQ ID NO: 78),
    • EphA4-A02-9-501 (SEQ ID NO: 376),
    • EphA4-A02-9-165 (SEQ ID NO: 379),
    • ECT2-A24-9-515 (SEQ ID NO: 80),
    • ECT2-A24-10-40 (SEQ ID NO: 100),
    • ECT2-A24-10-101 (SEQ ID NO: 101),
    • HIG2-A24-9-19 (SEQ ID NO: 110),
    • HIG2-A24-9-22 (SEQ ID NO: 111),
    • HIG2-A24-9-8 (SEQ ID NO: 387),
    • HIG2-A24-10-7 (SEQ ID NO: 112),
    • HIG2-A24-10-18 (SEQ ID NO: 394),
    • HIG2-A02-9-8 (SEQ ID NO: 114),
    • HIG2-A02-9-15 (SEQ ID NO: 116),
    • HIG2-A02-9-4 (SEQ ID NO: 117),
    • HIG2-A02-10-8 (SEQ ID NO: 121),
    • INHBB-A24-9-180 (SEQ ID NO: 395),
    • INHBB-A24-10-180 (SEQ ID NO: 133),
    • INHBB-A24-10-305 (SEQ ID NO: 135),
    • INHBB-A24-10-7 (SEQ ID NO: 137),
    • INHBB-A24-10-212 (SEQ ID NO: 426),
    • KIF20A-A24-9-305 (SEQ ID NO: 174),
    • KIF20A-A24-9-383 (SEQ ID NO: 178),
    • KIF20A-A24-10-304 (SEQ ID NO: 186),
    • KIF20A-A24-10-66 (SEQ ID NO: 194),
    • KNTC2-A24-9-309 (SEQ ID NO: 196),
    • KNTC2-A24-9-124 (SEQ ID NO: 202),
    • KNTC2-A24-9-154 (SEQ ID NO: 210),
    • KNTC2-A24-9-150 (SEQ 1D NO: 213),
    • KNTC2-A24-10-452 (SEQ ID NO: 214),
    • KNTC2-A24-10-227 (SEQ ID NO: 217),
    • KNTC2-A24-10-273 (SEQ ID NO: 223),
    • TTK-A02-9-462 (SEQ ID NO: 227),
    • TTK-A02-9-547 (SEQ ID NO: 228),
    • TTK-A02-9-719 (SEQ ID NO: 233),
    • TTK-A02-10-462 (SEQ ID NO: 254),
    • URLC-A02-9-206 (SEQ ID NO: 271),
    • URLC-A02-9-212 (SEQ ID NO: 272) and
    • URLC-A02-10-211 (SEQ ID NO: 288)
  • Accordingly, the possibility of unknown or undesirable immune responses with immunotherapy against these molecules is significantly reduced.
  • Regarding HLA antigens, the data presented here demonstrate that the uses of A-24 type or A-2 type antigens (which are said to be highly expressed among the Japanese) are favorable for obtaining effective results. The uses of subtypes such as A-2402 and A-0201 are even more preferable. Typically, in the clinic, the type of HLA antigen of the patient requiring treatment is investigated in advance, which, in turn, enables the selection of appropriate peptides having high levels of binding affinity to the patient antigen, or having cytotoxic T cell (CTL) inducibility by antigen presentation. Furthermore, in order to obtain peptides having high binding affinity and CTL inducibility, substitution, deletion, or addition of 1, 2, or several (e.g., up to 5) amino acids may be performed based on the amino acid sequence of the naturally occurring CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 partial peptide. Herein, the term "several" means refers to 5 or less, more preferably 3 or less. Furthermore, in addition to peptides that are naturally displayed, since the regularity of the sequences of peptides displayed by binding to HLA antigens is already known (Kubo RT, et al., (1994) J. Immunol., 152, 3913-24.; Rammensee HG, et al., (1995) Immunogenetics. 41:178-228.; Kondo A, et al., (1995) J. Immunol. 155:4307-12.), modifications based on such regularity can be performed on the immunogenic peptides of the invention. For example, peptides possessing high HLA-24 binding affinity in which the second amino acid from the N terminus substituted with phenylalanine, tyrosine, methionine, or tryptophan may be favorably used. Likewise, peptides whose C-terminal amino acid is substituted with phenylalanine, leucine, isoleucine, tryptophan, or methionine may also be used favorably. On the other hand, peptides possessing high HLA-A2 binding affinity in which the second amino acid from the N terminus substituted with leucine or methionine, and peptides whose C-terminal amino acid is substituted with valine or leucine may be used favorably. The substitution is performed not only at the terminus amino acids but also at the position of potential TCR recognition of peptides. Several studies have demonstrated that amino acid substitutions in a peptide can be equal to or better than the original, for example CAP1, p53 (264-272), Her-2/neu (369-377) or gp100 (209-217) (Zaremba et al. Cancer Res. 57, 4570-4577, 1997, T. K. Hoffmann et al. J Immunol. (2002) ., S. O. Dionne et al. Cancer Immunol immunother. (2003) 52: 199-206 and S. O. Dionne et al. Cancer Immunology, Immunotherapy (2004) 53, 307-314). Furthermore, 1 to 2 amino acids may be added to the N terminus and/or C terminus of the peptide.
  • However, when the peptide sequence is identical to a portion of the amino acid sequence of an endogenous or exogenous protein having a different function, side effects such as autoimmune disorders or allergic symptoms against specific substances may be induced. Therefore, it is preferable to avoid the situation wherein the immunogenic sequence matches the amino acid sequence of a known protein. This situation may be avoided by performing a homology search using available databases. If homology searches confirm that peptides in which 1, 2 or several different amino acids do not exist in nature, then the danger that modifications of the above-mentioned amino acid sequence that, for example, increase the binding affinity with HLA antigens, and/or increase the CTL inducibility can be avoided.
  • Although peptides having high binding affinity to the HLA antigens as described above are expected to be highly effective as cancer vaccines, the candidate peptides, which are selected according to the presence of high binding affinity as an indicator, must be examined for the actual presence of CTL inducibility. CTL inducibility may be routinely confirmed by inducing antigen-presenting cells carrying human MHC antigens (for example, B-lymphocytes, macrophages, and dendritic cells), or more specifically dendritic cells derived from human peripheral blood mononuclear leukocytes, and, after stimulation with the peptide of interest, mixing with CD8-positive cells and measuring the cytotoxic activity against the target cells. As the reaction system, transgenic animals produced to express a human HLA antigen (for example, those described in BenMohamed L, et al., (2000) Hum. Immunol.; 61(8):764-79 Related Articles, Books, Linkout.) may be used. For example, the target cells can be radiolabeled with 51Cr and such, and cytotoxic activity can be calculated from radioactivity released from the target cells. Alternatively, it can be examined by measuring IFN-gamma produced and released by CTL in the presence of antigen-presenting cells that carry immobilized peptides, and visualizing the inhibition zone on the media using anti-IFN-gamma monoclonal antibodies.
  • As a result of examining the CTL inducibility of peptides as described above, it was discovered that those peptides having high binding affinity to an HLA antigen did not necessarily have high inducibility. However, nonapeptides or decapeptides selected from the group of peptides having the amino acid sequences indicated by the following peptides showed particularly high CTL inducibility.
    • CDH3-A24-9-513 (SEQ ID NO: 19),
    • CDH3-A24-9-406 (SEQ ID NO: 22),
    • CDH3-A24-10-807 (SEQ ID NO: 30),
    • CDH3-A24-10-332 (SEQ ID NO: 34),
    • CDH3-A24-10-655 (SEQ ID NO: 344),
    • CDH3-A24-10-470 (SEQ ID NO: 358),
    • EphA4-A24-9-453 (SEQ ID NO: 41),
    • EphA4-A24-9-5 (SEQ ID NO: 44),
    • EphA4-A24-9-869 (SEQ ID NO: 46),
    • EphA4-A24-9-420 (SEQ ID NO: 48),
    • EphA4-A24-10-24 (SEQ ID NO: 78),
    • EphA4-A02-9-501 (SEQ ID NO: 376),
    • EphA4-A02-9-165 (SEQ ID NO: 379),
    • ECT2-A24-9-515 (SEQ ID NO: 80),
    • ECT2-A24-10-40 (SEQ ID NO: 100),
    • ECT2-A24-10-101 (SEQ ID NO: 101),
    • HIG2-A24-9-19 (SEQ ID NO: 110),
    • HIG2-A24-9-22 (SEQ ID NO: 111),
    • HIG2-A24-9-8 (SEQ ID NO: 387),
    • HIG2-A24-10-7 (SEQ ID NO: 112),
    • HIG2-A24-10-18 (SEQ ID NO: 394),
    • HIG2-A02-9-8 (SEQ ID NO: 114),
    • HIG2-A02-9-15 (SEQ ID NO: 116),
    • HIG2-A02-9-4 (SEQ ID NO: 117),
    • HIG2-A02-10-8 (SEQ ID NO: 121),
    • INHBB-A24-9-180 (SEQ ID NO: 395),
    • INHBB-A24-10-180 (SEQ ID NO: 133),
    • INHBB-A24-10-305 (SEQ ID NO: 135),
    • INHBB-A24-10-7 (SEQ ID NO: 137),
    • INHBB-A24-10-212 (SEQ ID NO: 426),
    • KIF20A-A24-9-305 (SEQ ID NO: 174),
    • KIF20A-A24-9-383 (SEQ ID NO: 178),
    • KIF20A-A24-10-304 (SEQ ID NO: 186),
    • KIF20A-A24-10-66 (SEQ ID NO: 194),
    • KNTC2-A24-9-309 (SEQ ID NO: 196),
    • KNTC2-A24-9-124 (SEQ ID NO: 202),
    • KNTC2-A24-9-154 (SEQ ID NO: 210),
    • KNTC2-A24-9-150 (SEQ ID NO: 213),
    • KNTC2-A24-10-452 (SEQ ID NO: 214),
    • KNTC2-A24-10-227 (SEQ ID NO: 217),
    • KNTC2-A24-10-273 (SEQ ID NO: 223),
    • TTK-A02-9-462 (SEQ ID NO: 227),
    • TTK-A02-9-547 (SEQ ID NO: 228),
    • TTK-A02-9-719 (SEQ ID NO: 233),
    • TTK-A02-10-462 (SEQ ID NO: 254),
    • URLC-A02-9-206 (SEQ ID NO: 271),
    • URLC-A02-9-212 (SEQ ID NO: 272) and
    • URLC-A02-10-211 (SEQ ID NO: 288)
  • As noted above, the present invention provides peptides having cytotoxic T cell inducibility, namely those having the amino acid sequence of SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof (i.e., those in which 1, 2, or several amino acids are substituted, deleted, or added).
  • It is preferable that the amino acid sequences composed of 9 or 10 amino acids indicated in SEQ ID NOs: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof do not match an amino acid sequence associated with another endogenous protein.
  • In particular, amino acid substitution to leucine or methionine at the second amino acid from the N terminus, amino acid substitution to valine or leucine at the C-terminal amino acid, and amino acid addition of 1 to 2 amino acids at the N terminus and/or C terminus are examples of preferred variants.
  • One of skill in the art will recognize that in addition to amino acid substitutions and additions, immunologically active fragments of the peptides may also be used in the methods of the invention. Methods for determining active fragments are well known in the art. CTL clones obtained by stimulation by these modified peptides can recognize the original peptides and cause damage for cells expressing the original peptides.
  • Peptides of the present invention can be prepared using well known techniques. For example, the peptides can be prepared synthetically, using either recombinant DNA technology or chemical synthesis. Peptides of the present invention may be synthesized individually or as longer polypeptides composed of two or more peptides. The peptides of the present invention are preferably isolated, i.e., substantially free of other naturally occurring host cell proteins and fragments thereof.
  • The peptides of the present invention may contain modifications, such as glycosylation, side chain oxidation, or phosphorylation; so long as the modifications do not destroy the biological activity of the peptides as described herein, namely the ability to binding to an HLA antigen and induce CTL. Other modifications include incorporation of D-amino acids or other amino acid mimetics that can be used, for example, to increase the serum half life of the peptides.
  • Moreover, this invention may contain a method of screening for a peptide which 1, 2, or several amino acids are substituted, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223, said method comprising the steps of:
    1. (a) comforming no significant sequence homology to the entire sequence of 1, 2 or several amino acids substitute;
    2. (b) measuring the CTL inducibility of the candidate substitute peptide; and
    3. (c) selecting the peptide which CTL inducibility is same to or higher than the original peptide.
  • For example, in prefered embodiments, the present invention provides a method of identifying for a peptide having an ability to induce CTL against cells expressing at leaset one tumor-associated antigen, wherein the tumor-associated antigen is antigen selected from the group consisting of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10, said method comprising the steps of:
    1. (i) providing or generating at least one candidate sequence which consists of an amino acid sequence modified by substituting, deleting, or adding one, two or several amino acid residues to an original amino acid sequence, wherein the original amino acid sequence is selected from the group consisting of SEQ ID NO: SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 2I0, 213, 214, 217 or 223;
    2. (ii) selecting the candidate sequence that does not have substantial significant homology with the peptides derived from any known human gene products other than said tumor-associated antigens;
    3. (iii) contacting a peptide consisting of the candidate sequence selected in step (ii) with antigen presenting cells;
    4. (iv) contacting the antigen presenting cells of step (c) with T-cells to evaluate the ability of the peptide to stimulate the T-cells; and
    5. (v) identifying the peptide of which CTL inducibility is same to or higher than a peptide consisting of the original amino acid sequence.
  • Preferably, the amino acid is substituted for a different amino acid in which the properties of the amino acid side-chain are conserved (a process known as conservative amino acid substitution). Examples of properties of amino acid side chains are hydrophobic amino acids (A, I, L, M, F, P, W, Y, V), hydrophilic amino acids (R, D, N, C, E, Q, G, H, K, S, T), and side chains having the following functional groups or characteristics in common: an aliphatic side-chain (G, A, V, L, I, P); a hydroxyl group containing side-chain (S, T, Y); a sulfur atom containing side-chain (C, M); a carboxylic acid and amide containing side-chain (D, N, E, Q); a base containing side-chain (R, K, H); and an aromatic containing side-chain (H, F, Y, W). Note, the parenthetic letters indicate the one-letter codes of amino acids. In the present invention, substantial significant homology is, for example, more than 90%, preferably 95%, more preferably 99% or 100% identity with a known human gene product to be compared.
  • The peptides of this invention can be prepared as a combination, which includes two or more of peptides of the invention, for use as a vaccine for a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, such a vaccine inducing CTL in vivo. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. The peptides may be in a cocktail or may be conjugated to each other using standard techniques. For example, the peptides can be expressed as a single polypeptide sequence. The peptides in the combination may be the same or different.
  • By administering the peptides of this invention, the peptides are presented at a high density on the HLA antigens of antigen-presenting cells, which, in turn, induces CTLs that specifically react toward the complex formed between the displayed peptide and the HLA antigen. Alternatively, antigen-presenting cells having immobilized the peptides of this invention on their cell surface, obtained by removing dendritic cells from the subjects, may be stimulated by the peptides of this invention. Re-administration of these cells to the respective subjects induces CTL, and, as a result, aggressiveness towards the target cells can be increased.
  • More specifically, the present invention provides drugs for treating and/or preventing proliferation, metastasis, and such of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, which include one or more of peptides of the present invention, or a polynucleotide encoding the peptides. The peptides or polynucleotides of the present invention find particular utility in the treatment of a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • The peptides of this invention can be administered to a subject directly, as a pharmaceutical composition that has been formulated by conventional formulation methods. In such cases, in addition to the peptides of this invention, carriers, excipients, and such that are ordinarily used for drugs can be included as appropriate, without particular limitations. The immunogenic compositions of this invention may be used for treatment and prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • The immunogenic compositions for treatment and/or prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, which include as the active ingredient one or more peptides of the present invention, can further include an adjuvant so that cellular immunity will be established effectively. Alternatively, they may be administered with other active ingredients, such as anti-cancer agents.
  • The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. Suitable formulations include granules. Suitable adjuvants are described in the literature (Johnson AG. (1994) Clin. Microbiol. Rev., 7:277-89.).
  • Exemplary adjuvants include, but are not limited to, aluminum phosphate, aluminum hydroxide, and alum. Furthermore, liposome formulations, granular formulations in which the drug is bound to few-mc m diameter beads, and formulations in which a lipid is bound to the peptide may be conveniently used. The method of administration may be oral, intradermal, subcutaneous, intravenous injection, or such, and may include systemic administration or local administration to the vicinity of the targeted tumor.
  • The dose of the peptide(s) of this invention can be adjusted appropriately according to the disease to be treated, age of the patient, weight, method of administration, and such. Though the dosage is ordinarily 0.001 mg to 1000 mg, preferably 0.01 mg to 100 mg, more preferably 0.1 mg to 10 mg, preferably administered once in a few days to few months, one skilled in the art can readily select the appropriate dose and method of administration, as, the selection and optimization of these parameters is well within routine skill.
  • The present invention further provides intracellular vesicles called exosomes, which present complexes formed between the peptides of this invention and HLA antigens on their surface. Exosomes can be prepared, for example, by using the methods described in detail in Published Japanese Translation of International Publication Nos. Hei 11-510507 and 2000-512161 , and are preferably prepared using antigen-presenting cells obtained from subjects who are targets of treatment and/or prevention. The exosomes of this invention can be inoculated as cancer vaccines, similarly to the peptides of this invention.
  • The type of HLA antigens used must match that of the subject requiring treatment and/or prevention. For example, in the Japanese population, HLA-A24 or HLA-A2, particularly HLA-A2402 or HLA-A0201, is often appropriate.
  • In some embodiments, the vaccine compositions of the present invention include a component which primes cytotoxic T lymphocytes. Lipids have been identified as agents capable of priming CTL in vivo against viral antigens. For example, palmitic acid residues can be attached to the epsilon-and alpha-amino groups of a lysine residue and then linked to an immunogenic peptide of the invention. The lipidated peptide can then be administered either directly, in a micelle or particle, incorporated into a liposome, or emulsified in an adjuvant. As another example of a lipid priming of CTL responses, E. coli lipoproteins, such as tripalmitoyl-S-glycerylcysteinlyseryl- serine (P3CSS), can be used to prime CTL when covalently attached to an appropriate peptide (see, e.g., Deres K, et al., (1989) Nature 342:561-4.).
  • The immunogenic compositions of the present invention may also include nucleic acids encoding one or more of the immunogenic peptides disclosed here. See, e.g., Wolff JA et al., (1990) Science 247:1465-8; U.S. Patent Nos. 5,580,859 ; 5,589,466 ; 5,804,566 ; 5,739,118 ; 5,736,524 ; 5,679,647 ; and WO 98/04720 . Examples of DNA-based delivery technologies include "naked DNA", facilitated (bupivicaine, polymers, peptide-mediated) delivery, cationic lipid complexes, and particle-mediated ("gene gun") or pressure-mediated delivery (see, e.g., U.S. Patent No. 5,922,687 ).
  • The immunogenic peptides of the invention can also be expressed by viral or bacterial vectors. Examples of suitable expression vectors include attenuated viral hosts, such as vaccinia or fowlpox. This approach involves the use of vaccinia virus, e.g., as a vector to express nucleotide sequences that encode the peptide. Upon introduction into a host, the recombinant vaccinia virus expresses the immunogenic peptide, and thereby elicits an immune response. Vaccinia vectors and methods useful in immunization protocols are described in, e.g., U.S. Patent No. 4,722,848 . Another suitable vector is BCG (Bacille Calmette Guerin). BCG vectors are described in Stover CK, et al., (1991) Nature 351:456-60. A wide variety of other vectors useful for therapeutic administration or immunization e.g., adeno and adeno-associated virus vectors, retroviral vectors, Salmonella typhi vectors, detoxified anthrax toxin vectors, and the like, are known in the art. See, e.g., Shata MT, et al., (2000) Mol. Med. Today 6:66-71; Shedlock DJ and Weiner DB., et al., (2000) J. Leukoc. Biol. 68:793-806; and Hipp JD, et al., (2000) In Vivo 14:572-85.
  • The present invention also provides methods of inducing antigen-presenting cells using one or more peptides of this invention. The antigen-presenting cells can be induced by inducing dendritic cells from the peripheral blood monocytes and then contacting (stimulating) them with one or more peptides of this invention in vitro, ex vivo or in vivo. When peptides of the present invention are administered to the subjects, antigen-presenting cells that have the peptides of this invention immobilized to them are induced in the body of the subject. Alternatively, after immobilizing the peptides of this invention to the antigen-presenting cells, the cells can be administered to the subject as a vaccine. For example, the ex vivo administration may include the steps of:
    1. a: collecting antigen-presenting cells from a subject, and
    2. b: contacting the antigen-presenting cells of step a with a peptide of the present invention.
  • Alternatively, according to the present invention, use of the peptides of this invention for manufacturing a pharmaceutical composition inducing antigen-presenting cells is provided. Further, the present invention also provides the peptide of the present invention for inducing antigen-presenting cells. The antigen-presenting cells obtained by step b can be administered to the subject as a vaccine.
  • This invention also provides a method for inducing antigen-presenting cells having a high level of cytotoxic T cell inducibility, in which the method includes the step of transferring genes composed of polynucleotide(s) encoding one or more peptides of this invention to antigen-presenting cells in vitro. The introduced genes may be in the form of DNAs or RNAs. For the method of introduction, without particular limitations, various methods conventionally performed in this field, such as lipofection, electroporation, and calcium phosphate method may be suitably used. More specifically, transfection may be performed as described in Reeves ME, et al., (1996) Cancer Res., 56:5672-7.; Butterfield LH, et al., (1998) J. Immunol., 161:5607-13.; Boczkowski D, et al., (1996) J. Exp. Med., 184:465-72.; Published Japanese Translation of International Publication No. 2000-509281 . By transferring the gene into antigen-presenting cells, the gene undergoes transcription, translation, and such in the cell, and then the obtained protein is processed by MHC Class I or Class II, and proceeds through a presentation pathway to present partial peptides.
  • The present invention further provides methods for inducing CTL using one or more peptides of this invention. When the peptides of this invention are administered to a subject, CTL are induced in the body of the subject, and the strength of the immune system targeting the cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer cells in the tumor tissues is thereby enhanced.
  • The cancers contemplated include, but are not limited to bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. Alternatively, the peptides of the present invention may be used in the context of an ex vivo therapeutic method, in which subject-derived antigen-presenting cells and CD8-positive cells or peripheral blood mononuclear leukocytes are contacted (stimulated) with one or more peptides of this invention in vitro, and, after inducing CTL, the cells are returned to the subject. For example, the method may include the steps of:
    • a: collecting antigen-presenting cells from a subject,
    • b: contacting the antigen-presenting cells of step a with a peptide of the present invention,
    • c: mixing the antigen-presenting cells of step b with CD8+ T cells and co-culturing so as to induce cytotoxic T-cells:, and
    • d: collecting CD8+ T cells from the co-culture of step c.
  • Alternatively, according to the present invention, use of the peptides of this invention for manufacturing a pharmaceutical composition inducing CTLs is provided. Further, the present invention also provides the peptide of the present invention for inducing CTLs. The CD8+ T cells having cytotoxic activity obtained by step d can be administered to the subject as a vaccine.
  • The present invention further provides isolated cytotoxic T cells induced using the peptides of this invention. The cytotoxic T cells, induced by stimulation with an antigen-presenting cell presenting one or more peptides of this invention, are preferably derived from subjects who are the target of treatment and/or prevention, and can be administered alone or in combination with other drugs, including one or more peptides of this invention or exosomes having anti-tumor activity. The obtained cytotoxic T cells act specifically against target cells presenting the peptides of this invention, or preferably the same peptide(s) used for induction. The target cells may be cells that express CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 endogenously, or cells that are transfected with CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 genes. Cells that present the peptides of this invention on the cell surface, due to stimulation with these peptides, can also become targets of attack.
  • The present invention also provides antigen-presenting cells presenting complexes formed between HLA antigens and one or more peptides of this invention. The antigen-presenting cells, obtained through contact with the peptides of this invention or the nucleotides encoding such peptides, are preferably derived from subjects who are the target of treatment and/or prevention, and can be administered as vaccines, alone or in combination with other drugs, including the peptides, exosomes, or cytotoxic T cells of the present invention.
  • The present invention also provides a composition composed of nucleic acids encoding polypeptides that are capable of forming a subunit of a T cell receptor (TCR), and methods of using the same. The TCR subunits have the ability to form TCRs that confer specificity to T cells for tumor cells presenting CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10. By using the known method in the art, the nucleic acids of alpha- and beta-chain as the TCR subunits of the CTL induced with one or more peptides of this invention may be identified ( WO2007/032255 and Morgan et al., J Immunol, 171, 3288 (2003)). The derivative TCRs preferably bind target cells displaying the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide with high avidity, and optionally mediate efficient killing of target cells presenting the CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide in vivo and in vitro.
  • The nucleic acids encoding the TCR subunits can be incorporated into suitable vectors e.g. retroviral vectors. These vectors are well known in the art. The nucleic acids or the vectors containing them usefully can be transferred into a T cell, which T cell is preferably from a patient. Advantageously, the invention provides an off-the-shelf composition allowing rapid modification of a patient's own T cells (or those of another mammal) to rapidly and easily produce modified T cells having excellent cancer cell killing properties.
  • Also, the present invention provides CTLs which are prepared by transduction with the nucleic acids encoding the TCR subunits polypeptides binding with CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 peptide e.g. SEQ ID NOs: 19, 22, 34, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 in the context of HLA-A24 or HLA-A2. The transduced CTLs are capable of homing to cancer cells in vivo, and expanded by well known culturing method in vitro (e.g., Kawakami et al., J Immunol., 142, 3452-3461 (1989)). The T cells of the invention can be used to form an immunogenic composition useful in treating or preventing cancer in a patient in need of therapy or protection ( WO2006/031221 ).
  • In the context of the present invention, the term "vaccine" (also referred to as an immunogenic composition) refers to a substance that induces anti-tumor immunity or suppresses cancers upon inoculation into animals. According to the present invention, polypeptides having the amino acid sequence of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223 were suggested to be HLA-A24 restricted epitope peptides and those of SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288 were suggested to be HLA-A2 restricted epitope peptides that may induce potent and specific immune response against cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10. The cancers contemplated include, but are not limited to bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • Thus, the present invention also encompasses a method of inducing anti-tumor immunity using polypeptides having the amino acid sequence of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 10I, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 or a variant thereof (i.e., including 1, 2, or several (e.g., up to 5) amino acid substitutions, deletions, or additions). In general, anti-tumor immunity includes immune responses such as follows:
    • an induction of cytotoxic lymphocytes against tumors containing cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10,
    • an induction of antibodies that recognize tumors containing cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, and
    • an induction of anti-tumor cytokine production.
  • Therefore, when a certain peptide induces any one of these immune responses upon inoculation into an animal, the peptide is decided to have anti-tumor immunity inducing effect. The induction of the anti-tumor immunity by a peptide can be detected by observing in vivo or in vitro the response of the immune system in the host against the peptide.
  • For example, a method for detecting the induction of cytotoxic T lymphocytes is well known. A foreign substance that enters the living body is presented to T cells and B cells by the action of antigen-presenting cells (APCs). T cells that respond to the antigen presented by APC in antigen specific manner differentiate into cytotoxic T cells (also referred to as cytotoxic T lymphocytes or CTLs) due to stimulation by the antigen, and then proliferate; this process is referred to herein as "activation" of T cells. Therefore, CTL induction by a certain peptide can be evaluated by presenting the peptide to a T cell by APC, and detecting the induction of CTL. Furthermore, APCs have the effect of activating CD4+ T cells, CD8+ T cells, macrophages, eosinophils and NK cells. Since CD4+ T cells are also important in anti-tumor immunity, the anti-tumor immunity inducing action of the peptide can be evaluated using the activation effect of these cells as indicators.
  • A method for evaluating the inducing action of CTL using dendritic cells (DCs) as APC is well known in the art. DC is a representative APC having the strongest CTL inducing action among APCs. In this method, the test polypeptide is initially contacted with DC and then this DC is contacted with T cells. Detection of T cells having cytotoxic effects against the cells of interest after the contact with DC shows that the test polypeptide has an activity of inducing the cytotoxic T cells. Activity of CTL against tumors can be detected, for example, using the lysis of 51Cr-labeled tumor cells as the indicator. Alternatively, it is well known to evaluate the degree of tumor cell damage using 3H-thymidine uptake activity or LDH (lactose dehydrogenase)-release as the indicator. Furthermore, it can be also examined by measuring IFN-gamma produced and released by CTL in the presence of antigen-presenting cells that carry immobilized peptides by visualizing using anti-IFN-gamma antibodies, such as an ELISPOT assay.
  • Apart from DC, peripheral blood mononuclear cells (PBMCs) may also be used as the APC. The induction of CTL is reported to be enhanced by culturing PBMC in the presence of GM-CSF and IL-4. Similarly, CTL has been shown to be induced by culturing PBMC in the presence of keyhole limpet hemocyanin (KLH) and IL-7.
  • The test polypeptides confirmed to possess CTL inducing activity by these methods are polypeptides having DC activation effect and subsequent CTL inducing activity. Therefore, polypeptides that induce CTL against cells expressed CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10 are useful as vaccines against diseases associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. Furthermore, APC that have acquired the ability to induce CTL against a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, by contacting with the polypeptides are useful as vaccines against the disease. Furthermore, CTL that have acquired cytotoxicity due to presentation of the polypeptide antigens by APC can be also used as vaccines against a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. Such therapeutic methods for a disease associating CDH3, EPHA4, ECT2, HTG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, using anti-tumor immunity due to APC and CTL, are referred to as cellular immunotherapy. The cancers contemplated include, but are not limited to, bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • Generally, when using a polypeptide for cellular immunotherapy, efficiency of the CTL-induction can be increased by combining a plurality of polypeptides having different structures and contacting them with DC. Therefore, when stimulating DC with protein fragments, it is advantageous to use a mixture of multiple types of fragments.
  • The induction of anti-tumor immunity by a polypeptide can be further confirmed by observing the induction of antibody production against tumors. For example, when antibodies against a polypeptide are induced in a laboratory animal immunized with the polypeptide, and when growth, proliferation and/or metastasis of tumor cells is suppressed by those antibodies, the polypeptide is determined to induce anti-tumor immunity.
  • Anti-tumor immunity can be induced by administering a vaccine of this invention, and the induction of anti-tumor immunity enables treatment and prevention of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers. Therapy against or prevention of the onset of a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, may include inhibition of the growth of cells expressing CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancer cells, involution of these cells and suppression of occurrence of these cells, e.g. cancer cells. Decrease in mortality of individuals having a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, decrease of the disease markers in the blood, alleviation of detectable symptoms accompanying the disease and such are also included in the therapy or prevention of the disease, e.g. cancers. Such therapeutic and preventive effects are preferably statistically significant, for example, observed at a significance level of 5% or less, wherein the therapeutic or preventive effect of a vaccine against a disease associating CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, is compared to a control without vaccine administration. For example, Student's t-test, the Mann-Whitney U-test or ANOVA may be used for determining statistical significance.
  • In that the present invention provides a method for treating, or preventing a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, the therapeutic compounds or compositions may be administered prophylactically or therapeutically to subjects suffering from or at risk of (or susceptible to) developing the disease. Such subjects may be identified using standard clinical methods. In the context of the present invention, prophylactic administration occurs prior to the manifestation of overt clinical symptoms of disease, such that a disease or disorder is prevented or alternatively delayed in its progression. In the context of the field of medicine, the term "prevent" encompasses any activity which reduces the burden of mortality or morbidity from disease. Prevention can occur at primary, secondary and tertiary prevention levels. While primary prevention avoids the development of a disease, secondary and tertiary levels of prevention encompass activities aimed at preventing the progression of a disease and the emergence of symptoms as well as reducing the negative impact of an already established disease by restoring function and reducing disease-related complications.
  • In the context of cancer treatment, the term "efficacious" refers to a treatment that leads to a decrease in size, prevalence or metastatic potential of cancer in a subject. When a treatment is applied prophylactically, "efficacious" means that the treatment retards or prevents occurrence of non cancer or alleviates a clinical symptom of cancer. The assessment of cancer can be made using standard clinical protocols. Furthermore, the efficaciousness of a treatment may be determined in association with any known method for diagnosing or treating cancer. For example, cancer can be diagnosed histopathologically or by identifying symptomatic anomalies.
  • The above-mentioned peptide, having immunological activity, or a polynucleotide or vector encoding such a peptide, may be combined with an adjuvant. An adjuvant refers to a compound that enhances the immune response against the peptide when administered together (or successively) with the peptide having immunological activity. Examples of suitable adjuvants include cholera toxin, salmonella toxin, alum and such, but are not limited thereto. Furthermore, a vaccine of this invention may be combined appropriately with a pharmaceutically acceptable carrier. Examples of such carriers are sterilized water, physiological saline, phosphate buffer, culture fluid and such. Furthermore, the vaccine may contain as necessary, stabilizers, suspensions, preservatives, surfactants and such. The vaccine is administered systemically or locally. Vaccine administration may be performed by single administration or boosted by multiple administrations.
  • When using APC or CTL as the vaccine of this invention, a disease associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or URLC10, e.g. cancers, can be treated or prevented, for example, by the ex vivo method. More specifically, PBMCs of the subject receiving treatment or prevention are collected, contacted ex vivo with a peptide of the present invention. Following the induction of APC or CTL, the cells may be administered to the subject. APC can be also induced by introducing a vector encoding the peptide into PBMCs ex vivo. APC or CTL induced in vitro can be cloned prior to administration. By cloning and growing cells having high activity of damaging target cells, cellular immunotherapy can be performed more effectively. Furthermore, APC and CTL isolated in this manner may be used for cellular immunotherapy not only against individuals from whom the cells are derived, but also against similar types of diseases in other individuals.
  • Aspects of the present invention are described in the following examples, which are presented only to illustrate the present invention and to assist one of ordinary skill in making and using the same. The examples are not intended in any way to otherwise limit the scope of the invention.
  • Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below.
    • EXAMPLES
  • Hereinafter, the present invention is exemplified, but not restricted, by the following Examples. However, materials, methods and such described herein only illustrate aspects of the invention and in no way are intended to limit the scope of the present invention. As such, materials, methods and such similar or equivalent to those described therein may be used in the practice or testing of the present invention.
    • MATERIALS AND METHODS Cell lines
  • A24-LCL cells (HLA-A24), human B-lymphoblastoid cell line, was established by transforming with Epstain-bar virus. T2 cell, COS7, A498, Caki-2 and HEK 293 were purchased from ATCC. Caki-1 and MIAPaca-2 were purchased from JCRB. PK-45P, PK-59, TE-5 and TE-6 were purchased from TKG. 293 T was purchased from GenHunter.
    • Candidate selection of peptide derived from CDH3, EPHA4, ECT2, HIG2. INHBB, KIF20A, KNTC2, TTK and URLC 10
  • 9-mer and 10-mer peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10 that bind to HLA-A*2402 or HLA-A*0201 molecule were predicted using the binding prediction software "BIMAS" (http://bimas.dcrt.nih.gov/cgi-bin/molbio/ken_parker comboform) (Parker KC, et al., (1994) J Immunol.;152(1):163-75.; Kuzushima K, et al., (2001) Blood.;98(6):1872-81.). These peptides were synthesized by Sigma (Sapporo, Japan) according to the standard solid phase synthesis method and purified by reversed phase HPLC. The purity (>90%) and the identity of the peptides were determined by analytical HPLC and mass spectrometry analysis, respectively. Peptides were dissolved in dimethylsulfoxide (DMSO) at 20 mg/ml and stored at -80 degrees C.
    • In vitro CTL Induction
  • Monocyte-derived dendritic cells (DCs) were used as antigen-presenting cells (APCs) to induce CTL responses against peptides presented on HLA. DCs were generated in vitro as described elsewhere (Nukaya I et al., (1999) ., Tsai V et al., (1997) J. Immunol 158:1796-802.). Briefly, peripheral blood mononuclear cells (PBMCs) isolated from a normal volunteer (HLA-A*2402 and/or HLA-A*0201) by Ficoll-Paque (Pharmacia) solution were separated by adherence to a plastic tissue culture flask (Becton Dickinson) so as to enrich them for the monocyte fraction. The monocyte-enriched population was cultured in the presence of 1000 U/ml of GM-CSF (Genzyme) and 1000 U/ml of IL-4 (Genzyme)in AIM-V(Invitrogen)containing 2% heat-inactivated autologous serum (AS). After 7 days in the culture, the cytokine-generated DCs were pulsed with 20 micro g/ml of the synthesized peptides in the presence of 3 micro g/ml of beta 2-microglobulin for 4 hrs at 20 degrees C in AIM-V. These peptide-pulsed DCs were then inactivated by MMC (30micro g/ml for 30 mins) and mixed at a 1:20 ratio with autologous CD8+ T cells, obtained by positive selection with Dynabeads M-450 CD8 (Dynal) and DETACHa BEAD™ (Dynal). These cultures were set up in 48-well plates (Corning); each well contained 1.5x104 peptide-pulsed DCs, 3x105 CD8+ T cells and 10 ng/ml of IL-7 (Genzyme) in 0.5 ml of AIM-V/2% AS. Three days later, these cultures were supplemented with IL-2 (CHIRON) to a final concentration of 20 IU/ml. On day 7 and 14, the T cells were further restimulated with the autologous peptide-pulsed DCs. The DCs were prepared each time by the same way described above. CTL was tested against peptide-pulsed A24-LCL cells or T2 cells after the 3rd round of peptide stimulation on day 21.
    • CTL Expansion Procedure
  • CTLs were expanded in culture using the method similar to that described by Riddell SR, et al., (Walter EA et al., (1995) N Engl J Med 333:1038-44.; Riddel SR, et al., (1996) Nature Med. 2:216-23.). A total 5x104 of CTLs were resuspended in 25 ml of AIM-V/5% AS with 2 kinds of human B-lymphoblastoid cell lines, inactivated by MMC, in the presence of 40 ng/ml of anti-CD3 monoclonal antibody (Pharmingen). One day after initiating the cultures, 120 IU/ml of IL-2 were added to the cultures. The cultures were fed with fresh AIM-V/5% AS containing 30 IU/ml of IL-2 on days 5, 8 and 11.
    • Establishment of CTL clones
  • The dilutions were made to have 0.3, 1, and 3 CTLs/well in 96 round-bottomed micro titer plate (Nalge Nunc International). CTLs were cultured with 7x104 cells/well of 2 kinds of human B-lynphoblastoid cell lines, 30ng/ml of anti-CD3 antibody, and 125 U/ml of IL-2 in total of 150micro 1/well of AIM-V containing 5%AS. 50 micro 1 /well of IL-2 was added to the medium 10 days later so that IL-2 became 125 U/ml in the final concentration. CTL activity of CTLs was tested on the 14th day, and CTL clones were expanded using the same method above.
    • Specific CTL activity
  • To examine the specific CTL activity, IFN-gamma ELISPOT assay and IFN-gamma ELISA assay were performed.
  • Briefly, peptide-pulsed A24-LCL or T2 cell (1x104/well) was prepared as stimulator cells. Cultured Cells in 48 wells or CTL clones after limiting dilution were used as responder cells. IFN-gamma ELISPOT assay and ELISA assay were performed under manufacture procedure.
    • Establishment of the cells forcibly expressing either or both of the target gene and HLA-A02 or HLA-A24
  • The cDNA encoding an open reading frame of taget genes or HLA-A02 or HLA-A24 was amplified by PCR. The PCR-amplified product was cloned into pcDNA3.1 myc-His vector (Invitrogen). The plasmids were transfected into the taget genes, HLA-A02 and HLA-A24-null nomal human cell line COS7 or 293T using lipofectamine (Invitrogen) according to the manufacturer's recommended procedures. Alternatively, the plasmid contained the target genes were transfected into A24-LCL by electroporation using GenePulserII (Biorad). Briefly, 2.5x106 A24-LCL cells were pulsed with 10 mcg prasmid at 140V and 1000 micro F. After 2days from transfection, the transfected cells were treated with Cell dissociation solution and used as the target cells for CTL activity assay.
    • Cytotoxicity Assay
  • Cytotoxic activity was evaluated by a four-hour 51Cr release assay. The target cells were pulsed with a 20 micro g/mL concentration of peptide overnight. The target cells were labeled with 100 micro Ci of Na2 51CrO4 at 37 degrees C for one hour, and then washed three times with RPMI1640. The target cells (1x 104/100 micro L) and 100 micro L of effector cells at various numbers with a total volume of 200 micro L were placed into a round-bottomed 96-well microtiter plate (Coming), and cultured at 37 degrees C in a CO2 incubator for four hours. After culturing, 100 micro L of the supernatant was collected from each well, and measured the radioactivity using a gamma counter. Spontaneous release was the radioactivity from the target cells with medium in the absence of effector cells, and maximum releasewas the radioactivity from the target cells with 1 M HCl.
  • The Percentage of specific cytotoxicity was determined by calculating as following formula: % Specific lysis = experimental release - spontaneous release / maximum release - spontaneous release ⁢ x 100.
    Figure imgb0001
    • RESULTS
  • Enhanced CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and
    • URLC10 expression in cancers
  • The global gene expression profile data obtained from various cancers using cDNA-microarray revealed that the expression of the following genes was elevated.
    CDH3 (GenBank Accession No. NM_001793; SEQ ID Nos.1 2),
    EPHA4 (GenBank Accession No. L36645; SEQ ID Nos.3, 4),
    ECT2 (GenBank Accession No. AY376439; SEQ ID Nos.5, 6),
    HIG2 (GenBank Accession No. NM_013332; SEQ ID Nos.7, 8),
    INHBB (GenBank Accession No. NM_002193; SEQ ID Nos.9, 10),
    KIF20A (GenBank Accession No. NM_005733; SEQ ID Nos.11, 12),
    KNTC2 (GenBank Accession No. AF017790; SEQ ID Nos.13, 14),
    TTK (GenBank Accession No. NM_003318; SEQ ID Nos.15, 16) and URLC10 (GenBank Accession No. NM_017527; SEQ ID Nos.17, 18) CDH3 expression was validly elevated in the following cancers in comparison with corresponding normal tissue.
    • 26 out of 34 bladder cancer,
    • 17 out of 19 cervical cancer,
    • 19 out of 19 cholangincellular carcinoma,
    • 30 out of 34 colorectal cancer,
    • 20 out of 21 endometriosis,
    • 13 out of 20 gastric cancer,
    • 7 out of 8 diffuse-type gastric cancer,
    • 36 out of 37 NSCLC,
    • 16 out of 16 pancreatic cancer,
    • 21 out of 21 soft tissue tumor and
    • 10 out of 10 testicular tumor
  • EPHA4 expression was validly elevated in the following cancers in comparison with corresponding normal tissue.
    • 14 out of 34 bladder cancer,
    • 8 out of 14 cervical cancer,
    • 10 out of 25 cholangincellular carcinoma,
    • 5 out of 15 endometriosis,
    • 5 out of 8 diffuse-type gastric cancer,
    • 5 out of 5 ovarian cancer,
    • 14 out of 14 pancreatic cancer,
    • 20 out of 51 prostate cancer and
    • 14 out of 23 soft tissue tumor
  • ECT2 expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
    • 17 out of 19 bladder cancer,
    • 5 out of 12 breast cancer,
    • 14 out of 14 cervical cancer,
    • 13 out of 13 cholangiocellular carcinoma,
    • 5 out of 5 CML,
    • 7 out of 8 colorectal cancer,
    • 12 out of 16 esophageal cancer,
    • 6 out of 16 NSCLC,
    • 8 out of 10 lymphoma,
    • 1 out of 1 pancreatic cancer,
    • 10 out of 13 prostate cancer,
    • 3 out of 6 renal carcinoma and
    • 12 out of 13 SCLC cancer
    • HIG2 expression was validly elevated in 19 out of 20 renal cancer and 7 out of 9 soft tissue tumor in comparing with corresponding normal tissue.
  • INHBB expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
    • 10 out of 21 cholangiocellular carcinoma,
    • 12 out of 12 esophageal cancer,
    • 10 out of 13 NSCLC,
    • 22 out of 24 renal carcinoma,
    • 8 out of 14 SCLC cancer and
    • 45 out of 49 soft tissue tumor
  • KIF20A expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
    • 31 out of 31 bladder cancer,
    • 38 out of 61 breast cancer,
    • 10 out of 11 cholangiocellular carcinoma,
    • 7 out of 19 esophageal cancer,
    • 21 out of 22 NSCLC,
    • 6 out of 6 ovarian cancer,
    • 17 out of 36 prostate cancer,
    • 6 out of 11 renal carcinoma and
    • 15 out of 15 SCLC
  • KNTC2 expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
    • 30 out of 32 bladder cancer,
    • 47 out of 56 breast cancer,
    • 10 out of 10 cervical cancer,
    • 16 out of 22 cholangioncellular carcinoma,
    • 17 out of 37 CML,
    • 3 out of 10 colorectal cancer,
    • 11 out of 46 esophagus cancer,
    • 15 out of 19 NSCLC,
    • 7 out of 8 lymphoma,
    • 20 out of 24 osteosarcoma,
    • 3 out of 5 ovarian cancer,
    • 2 out of 2 pancreatic cancer,
    • 15 out of 37 prostate cancer,
    • 14 out of 19 renal carcinoma,
    • 15 out of 15 SCLC and
    • 40 out of 59 soft tissue tumor
  • TTK expression was validly elevated in the following cancers in comparing with corresponding normal tissue.
    • 27 out of 27 bladder cancer,
    • 25 out of 30 breast cancer,
    • 15 out of 16 cervical cancer,
    • 10 out of 10 cholangiocellular carcinoma,
    • 5 out of 7 CML,
    • 6 out of 10 colorectal cancer,
    • 24 out of 44 esophageal cancer,
    • 8 out of 15 liver cancer,
    • 12 out of 12 NSCLC,
    • 6 out of 6 lymphoma,
    • 13 out of 16 osteoblastoma,
    • 12 out of 17 prostate cancer,
    • 15 out of 15 SCLC and
    • 16 out of 33 soft tissue tumor
  • URLC10 expression was validly elevated in the following cancers in comparing with corresponding normal tissue
    • 29 out of 29 bladder cancer,
    • 15 out of 16 cervical cancer,
    • 7 out of 7 cholangiocellular carcinoma,
    • 7 out of 19 esophageal cancer,
    • 3 out of 3 gastric cancer, 24 out of 27 NSCLC,
    • 15 out of 19 osteosarcoma,
    • 4 out of 5 pancreatic cancer,
    • 33 out of 43 soft tissue tumor.
    Figure imgb0002
     Prediction of HLA-A24 or HLA-A2 binding peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK or URLC10
  • Table 2 sets forth the HLA-A*2402 binding peptides for CDH3 in order of binding affinity. Table 2A sets forth 9-mer peptides derived from CDH3 and Table 2B sets forth 10-mer peptides derived from CDH3.
  • Table 3 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for EPHA4 in order of binding affinity. Table 3A sets forth the HLA-A*2402 binding 9-mer peptides derived from EPHA4, Table 3B shows the HLA-A*2402 binding 10-mer peptides derived from EPHA4 and Table 3C sets forth the HLA-A*0201 binding 9-mer peptides derived from EPHA4.
  • Table 4 sets forth the HLA-A*2402 binding peptides for ECT2 in order of binding affinity. Table 4A sets forth 9-mer peptides derived from ECT2 and Table 4B shows 10-mer peptides derived from ECT2.
  • Table 5 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for HIG2, Table 5A sets forth the HLA-A*2402 binding 9-mer peptides derived from HIG2, Table 5B sets forth the HLA-A*2402 binding 10-mer peptides derived from HIG2, Table 5C sets forth the HLA-A*0201 binding 9-mer peptides derived from HIG2, and Table 5D sets forth HLA-A*0201 binding 10-mer peptides derived from HIG2.
  • Table 6 sets forth the HLA-A*2402 and HLA-A*0201 binding peptides for INHBB, Table 6A shows the HLA-A*2402 binding 9-mer peptides derived from INHBB, Table 6B sets forth the HLA-A*2402 binding 10-mer peptides derived from INHBB, Table 6C sets forth the HLA-A*0201 binding 9-mer peptides derived from INHBB, and Table 6D sets forth HLA-A*0201 binding 10-mer peptides derived from INHBB.
  • Table 7 sets forth the HLA-A*2402 binding peptides for KIF20A in order of binding affinity. Table 7A sets forth 9-mer peptides derived from KIF20A and Table 7B sets forth 10-mer peptides derived from KIF20A.
  • Table 8 sets forth the HLA-A*2402 binding peptides for KNTC2 in order of binding affinity. Table 8A sets forth 9-mer peptides derived from KNTC2 and Table 8B sets forth 10-mer peptides derived from KNTC2.
  • Table 9 sets forth the HLA-A*0201 binding peptides for TTK in order of binding affinity. Table 9A sets forth 9-mer peptides derived from TTK and Table 9B sets forth 10-mer peptides derived from TTK.
  • Table 10 sets forth the HLA-A*0201 binding peptides for URLC10 in order of binding affinity. Table 10A sets forth 9-mer peptides derived from URLC10 and Table 10B sets forth 10-mer peptides derived from URLC10.
    • Explanation and definition about the terms in tables
  • Start position indicates the number of amino acid from N-terminal.
  • Binding score is derived from "BIMAS" described in Materials and Methods.
  • Positive donor number indicates the number of doner whoes CD8+-T-cells can be induced to the specific CTL by the ex vivo stimulation with antigen-presenting cells. This is shown as (positive donor number / hole donor nember).
  • Positive well number indicates the number of wells where specific IFN-gamma production can be detected by IFN-gamma ELISPOT assay. 4 to 8 wells can be prepared from one donor. This is shown as (positive wells number / the number of hole wells tested by IFN-gamma ELISPOT assay).
  • Positve CTL line indicates the number of CTL line established from positive wells. The generation of CTL line is determined by ELISA. This is shown as (established CTL line number / the number of positive wells tested by IFN-gamma ELISPOT assay).
  • No positive donor is not defined by no detectable positive wells, but by no established CTL line.
  • The peptides showed by bold character in tables possesses the stimulation activity of the T cells.
  • No data at positive donor number, positive well nember and positive CTL line indicating "-" means that the peptides can't be synthesized for any reason. [Table 2A-1]
    HLA-A*2402 binding 9-mer peptides derived from CDH3
    Strat position Amino acid sequence Binding Score Positive donor number Positive well number Positive CTL line SEQ ID NO.
    513 IYEVMVLAM 37.5 1/3 19
    667 LFLLLVLLL 36 - - - 20
    30 VFREAEVTL 24 0/3 1/22 0/1 21
    406 LYVEVTNEA 16.632 1/3 22
    332 KYEAHVPEN 16.5 0/3 1/22 0/1 23
    180 KYELFGHAV 15 0/3 1/22 0/1 24
    85 RSLKERNPL 14.4 0/3 1/22 0/1 25
    5 RGPLASLLL 12 0/3 2/22 0/2 26
    652 KGGFILPVL 11.2 0/3 0/22 - 27
    248 TYNGWAYS 10.5 0/3 2/22 0/2 28
    65 LFSTDNDDF 10 0/3 0/22 - 29
    94 KIFPSKRIL 9.6 0/1 0/8 - 306
    221 RGSVLEGVL 9.6 0/1 0/8 - 307
    668 FLLLVLLLL 8.4 - - - 308
    754 IGNFIIENL 8.4 - - - 309
    311 TAVAVVEIL 8.4 0/1 0/8 - 310
    557 NQSPVRQVL 8.064 0/1 0/8 - 311
    611 KQDTYDVHL 8 0/1 0/8 - 312
    781 DYEGSGSDA 7.5 0/1 0/8 - 313
    165 GWLLLNKPL 7.2 0/1 0/8 - 314
    [Table 2A-2]
    656 ILPVLGAVL 7.2 0/1 0/8 - 315
    770 TAPPYDTLL 7.2 0/1 0/8 - 316
    602 WLSLKKFL 7.2 0/1 0/8 - 317
    665 ALLFLLLVL 7.2 - - - 318
    410 VTNEAPFVL 7.2 0/1 0/8 - 319
    662 AVLALLFLL 7.2 - - - 320
    613 DTYDVHLSL 6.72 0/1 0/8 - 321
    6 GPLASLLLL 6 0/1 0/8 - 322
    564 VLNITDKDL 6 0/1 0/8 - 323
    159 AVEKETGWL 6 0/1 0/8 - 324
    511 NNIYEVMVL 6 0/1 0/8 - 325
    11 LLLLQVCWL 6 - - - 326
    57 GCPGQEPAL 6 0/1 0/8 - 327
    293 EYTLTIQAT 6 0/1 0/8 - 328
    79 ETVQERRSL 6 0/1 0/8 - 329
    475 SYRILRDPA 6 0/1 0/8 - 330
    493 GQVTAVGTL 6 0/1 0/8 - 331
    661 GAVLALLFL 6 0/1 0/8 - 332
    388 GILTTRKGL 6 0/1 0/8 - 333
    382 HPESNQGIL 6 0/1 0/8 - 334
    663 VLALLFLLL 5.76 - - - 335
    598 EGDTVVLSL 5.6 0/1 0/8 - 336
    278 TISVISSGL 5.6 0/1 2/8 0/2 337
    659 VLGAVLALL 5.6 0/1 0/8 - 338
    811 EWGSRFKKL 5.28 0/1 0/8 - 339
    445 KVVEVQEGI 5.04 0/1 0/8 - 340
    614 TYDVHLSLS 5 0/1 0/8 - 341
    142 FYSITGPGA 5 0/1 0/8 - 342
    246 IYTYNGVVA 5 0/1 0/8 - 343
    [Table 2B-1]
    HLA-A*2402 binding 10-mer pe tides derived from CDH3
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    807 DYLNeWGSRF 150 1/3 30
    248 TYNGvVAYSI 105 0/3 4/22 0/4 31
    667 LFLLIVLLLL 42 - - - 32
    397 DFEAkNQHTL 30 0/3 2/22 0/2 33
    332 KYEAhVPENA 21 1/3 34
    180 KYELFGHAVS 15 0/3 2/22 0/2 35
    510 RNNIYEVMVL 12 0/3 4/22 0/4 36
    5 RGPLASLLLL 12 0/3 1/22 0/1 37
    477 RILRDPAGWL 12 0/3 1/22 0/1 38
    556 CNQSPVRQVL 10.08 0/3 2/22 0/2 39
    655 FILPvLGAVL 8.64 1/3 344
    662 AVLAlLFLLL 8.64 - - - 345
    [Table 2B-2]
    277 GTISvISSGL 8.4 0/3 0/20 - 346
    781 DYEGsGSDAA 7.5 0/3 0/20 - 347
    601 TVVLsLKKFL 7.2 0/3 3/20 0/3 348
    158 FAVEkETGWL 7.2 0/3 0/20 - 349
    665 ALLFILLVLL 7.2 - - - 350
    259 SQEPkDPHDL 7.2 0/3 0/20 - 351
    664 LALLfLLLVL 7.2 - - - 352
    42 GAEQePGQAL 7.2 0/3 1/20 0/1 353
    661 GAVLaLLFLL 7.2 - - - 354
    595 VNEEgDTVVL 7.2 0/2 0/12 - 355
    340 NAVGhEVQRL 7.2 0/2 0/12 - 356
    411 TNEApFVLKL 6.6 0/2 0/12 - 357
    470 ENQKiSYRIL 6 1/2 358
    10 SLLLIQVCWL 6 0/2 1/12 0/1 359
    721 GLEArPEVVL 6 0/2 2/12 0/2 360
    345 EVQRITVTDL 6 0/2 4/12 0/4 361
    2 GLPRgPLASL 6 0/2 3/12 0/3 362
    657 LPVLgAVLAL 6 - - - 363
    563 QVLNiTDKDL 6 0/2 1/12 0/1 364
    159 AVEKeTGWLL 6 0/2 2/12 0/2 365
    492 SGQVtAVGTL 6 0/2 - - 366
    387 QGILtTRKGL 6 0/2 - - 367
    525 SPPTtGTGTL 6 0/2 2/12 0/2 368
    358 NSPAwRATYL 6 0/2 2/12 0/2 369
    122 GPFPqRLNQL 5.76 0/2 3/12 0/3 370
    753 EIGNfIIENL 5.6 0/2 1/12 0/1 371
    310 TTAVaVVEIL 5.6 - - - 372
    246 IYTYnGWAY 5 0/2 2/12 0/2 373
    805 DYDYINEWGS 5 0/2 0/12 - 374
    [Table 3A]
    HLA-A*2402 binding 9-mer peptides derived from EPHA4
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    97 VYIEIKFTL 504 0/2 1/16 0/1 40
    453 RYSYALAWL 400 2/3 41
    25 VYPANEVTL 300 0/3 0/22 - 42
    384 HYTPQQNGL 288 0/3 1/22 0/1 43
    5 FYFALFSCL 288 1/2 44
    519 GYGDFSEPL 240 0/3 3/22 0/3 45
    869 KFGQIVNML 67.2 1/3 46
    777 AYTTRGGKI 55 0/3 1/22 0/1 47
    420 KYNPNPDQS 18 1/3 48
    749 RNILVNSNL 16.8 0/3 1/22 0/1 49
    734 KYLSDMSYV 15 0/3 0/22 - 50
    879 KLIRNPNSL 14.4 0/3 0/22 - 51
    926 RYKDNFTAA 14.4 0/3 0/22 - 52
    834 KAIEEGYRL 14.4 0/3 0/22 - 53
    574 KYSKAKQEA 13.2 0/3 0/22 - 54
    184 AFQDVGACI 12.6 0/3 1/22 0/1 55
    252 WLVPIGNCL 12.096 0/3 0/22 - 56
    326 RPPSAPLNL 12 0/3 0/22 - 57
    203 KCPLTVRNL 12 0/3 0/22 - 58
    360 SYNVVCKKC 11.55 0/3 0/22 - 59
    [Table 3B]
    HLA-A*2402 binding 10-mer pe tides derived from EPHA4
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    25 VYPANEVTLL 300 0/3 0/22 - 60
    244 MYCGADGEWL 200 0/3 1/22 0/1 61
    657 GYTDKQRRDF 120 0/3 1/22 0/1 62
    5 FYFAIFSCLF 100 - - - 63
    102 KFTLRDCNSL 48 0/3 1/22 0/1 64
    818 SYGERPYWDM 30 0/3 2/22 0/2 65
    4 IFYFALFSCL 28.8 - - - 66
    808 SYGIVMWEVM 25 - - - 67
    630 EFGEVCSGRL 24 0/3 0/22 - 68
    420 KYNPNPDQSV 21.6 0/3 0/22 - 69
    930 NFTAAGYTTL 20 0/2 0/16 - 70
    675 QFDHPNIIHL 20 0/3 0/22 - 71
    708 AFLRKNDGRF 15 0/3 0/22 - 72
    579 KQEADEEKHL 12 0/3 1/22 0/1 73
    727 RGIGSGMKYL 12 0/3 0/22 - 74
    96 RVYIEIKFTL 11.2 0/2 1/16 0/1 75
    507 SYVFHVRART 10.5 0/3 1/22 0/1 76
    251 EWLVPIGNCL 10.08 0/3 0/22 - 77
    24 RVYPANEVTL 9.6 1/3 78
    699 EYMENGSLDA 9 0/3 0/22 - 79
    [Table 3C]
    HLA-A*0201 binding 9-mer peptides derived from EPHA4
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    8 ALFSCLFGI 514.942 - - - 375
    501 GLNPLTSYV 382.536 1/1 376
    12 CLFGICDAV 126.098 0/1 1/5 0/1 377
    977 QMHGRMVPV 115.534 0/1 1/5 0/1 378
    165 KLNTEIRDV 111.979 1/1 379
    252 WLVPIGNCL 98.267 0/1 1/5 0/1 380
    879 KLIRNPNSL 74.768 0/1 1/5 0/1 381
    559 VVILIAAFV 56.902 - - - 382
    812 VMWEVMSYG 39.386 0/1 0/5 - 383
    728 GIGSGMKYL 37.157 0/1 0/5 - 384
    750 NILVNSNLV 35.385 0/1 1/5 0/1 385
    937 TTLEAVVHV 33.705 0/1 1/5 0/1 386
    [Table 4A]
    HLA-A*2402 binding 9-mer peptides derived from ECT2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    515 TYPPFVNFF 216 1/1 80
    140 LYCTSMMNL 200 0/1 0/8 - 81
    298 LYVVKQEWF 150 0/1 0/8 - 82
    435 NYVNILATI 105 0/1 0/8 - 83
    773 IYTADPESF 100 0/1 0/8 - 84
    110 LYKADCRVI 50 0/1 0/8 - 85
    739 SFQMTSDEL 33 0/1 0/8 - 86
    504 IFLKYSKDL 30 0/1 0/8 - 87
    867 FFERRSHTL 30 0/1 0/8 - 88
    178 DFNSKVTHL 30 0/1 0/8 - 89
    61 KQEELIKAL 17.28 0/1 0/8 - 90
    657 RGEQVTLFL 16.8 0/1 2/8 0/2 91
    568 RLPSVALLL 16.8 0/1 0/8 - 92
    550 KPECGRQSL 14.4 0/1 0/8 - 93
    470 IFGSIPDIF 14 0/1 0/8 - 94
    116 RVIGPPVVL 12 0/1 0/8 - 95
    507 KYSKDLVKT 11 0/1 0/8 - 96
    223 DFYAAVDDF 10 0/1 0/8 - 97
    [Table 4B]
    HLA-A*2402 binding 10-mer peptides derived from ECT2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    322 LYEKaNTPEL 330 0/1 0/8 - 98
    435 NYVNiLATII 90 0/1 0/8 - 99
    40 SYVEeEMPQI 90 1/1 100
    101 DFQDsVFNDL 72.576 1/1 101
    866 SFFErRSHTL 24 0/1 0/8 - 102
    811 SFSKTPKRAL 20 0/1 1/8 0/1 103
    268 KYLPIGDERC 18 0/1 0/8 - 104
    84 EFEGIDSPEF 16.5 0/1 1/8 0/1 105
    236 KVPPfQDCIL 14.4 0/1 0/8 - 106
    728 RPPTeQANVL 14.4 0/1 0/8 - 107
    507 KYSKdLVKTY 12 0/1 0/8 - 108
    281 VVEEnIVKDL 10.08 0/1 0/8 - 109
    [Table 5A]
    HLA-A*2402 binding 9-mer peptides derived from HIG2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    19 IFVRVMESL 42 1/3 110
    22 RVMESLEGL 14.4 1/3 111
    8 YLLGVVLTL 8.4 1/3 387
    7 LYLLGVVLT 7.5 0/2 3/15 0/3 388
    23 VMESLEGLL 7.2 0/2 0/16 - 389
    9 LLGVVLTLL 5.6 - - - 390
    [Table 5B]
    Table 5B HLA-A*2402 binding 10-mer pe ptides derived from HIG2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    7 LYLLGVVLTL 420 1/3 112
    22 RVMESLEGLL 17.28 0/3 4/24 0/4 113
    8 YLLGVVLTLL 8.4 - - - 391
    5 LNLYLLGVVL 7.2 0/2 0/12 - 392
    46 LANTEPTKGL 6 0/2 0/14 - 393
    18 SIFVRVMESL 5.6 1/2 394
    [Table 5C]
    HLA-A*0201 binding 9-mer peptides derived from HIG2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    8 YLLGVVLTL 836.253 1/1 114
    13 VLTLLSIFV 650.311 0/1 0/12 - 115
    15 TLLSIFVRV 488.951 1/1 116
    4 VLNLYLLGV 271.948 1/1 117
    9 LLGVVLTLL 83.527 0/1 0/12 - 118
    22 RVMESLEGL 31.957 0/1 0/12 - 119
    6 NLYLLGVVL 28.027 0/1 0/12 - 120
    [Table 5D]
    HLA-A*0201 binding 10-mer peptides derived from HIG2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    8 YLLGvVLTLL 836.253 1/1 121
    12 VVLTILSIFV 210.538 - - - 122
    29 GLLEsPSPGT 113.047 0/1 0/12 - 123
    6 NLYLIGVVLT 54.847 - - - 124
    4 VLNLyLLGVV 14.495 0/1 0/12 - 125
    15 TLLSiFVRVM 13.174 0/1 0/12 - 126
    18 SIFVrVMESL 12.248 0/1 0/12 - 127
    14 LTLLsIFVRV 11.545 - - - 128
    [Table 6A]
    HLA-A*2402 binding 9-mer peptides derived from INHBB
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    383 LYFDDEYNI 60 0/3 0/20 - 129
    238 LFERGERRL 30 0/3 1/19 0/1 130
    7 RALGAACLL 12 0/3 0/21 - 131
    388 EYNIVKRDV 10.5 0/3 0/18 - 132
    180 LYLKLLPYV 9 1/2 395
    163 ISNEGNQNL 8.64 0/1 0/8 - 396
    223 RSGWHTFPL 8 0/1 0/6 - 397
    176 ASLWLYLKL 7.92 0/1 0/7 - 398
    338 AYLAGVPGS 7.5 0/1 1/7 0/1 399
    213 NMVEKRVDL 7.2 0/1 0/8 - 400
    102 AMVTALRKL 6.6 0/1 0/8 - 401
    250 VQCDSCQEL 6.336 0/1 0/8 - 402
    369 NSCCIPTKL 6.16 0/1 0/8 - 403
    330 NYCEGSCPA 6 0/1 0/7 - 404
    172 FVVQASLWL 6 0/1 0/8 - 405
    355 VNQYRMRGL 6 0/1 0/8 - 406
    307 QFFIDFRLI 6 0/1 0/7 - 407
    14 LLLLAAGWL 6 - - - 408
    306 QQFFIDFRL 5.6 0/1 0/6 - 409
    170 NLFVVQASL 5.6 0/1 0/7 - 410
    327 YYGNYCEGS 5 0/1 1/8 0/1 411
    [Table 6B]
    HLA-A*2402 binding 10-mer peptides derived from INHBB
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    180 LYLKLLPYVL 360 1/3 133
    171 LFVVQASLWL 30 - - - 134
    305 RQQFFIDFRL 16.8 1/3 135
    73 DFLEAVKRHI 12.6 0/3 4/20 0/4 136
    7 RALGAACLLL 12 1/3 137
    273 RPFVVVQARL 11.2 0/3 1/20 0/1 138
    338 AYLAGVPGSA 10 0/3 2/20 0/2 139
    169 QNLFvVQASL 8.4 0/1 1/6 0/1 412
    249 DVQCdSCQEL 7.92 0/1 4/6 0/4 413
    173 VVQAsLWLYL 7.2 0/1 0/6 - 414
    383 LYFDdEYNIV 7.2 0/1 0/6 - 415
    229 FPLTeAIQAL 7.2 0/1 1/6 0/1 416
    299 RTNLcCRQQF 7.2 0/1 5/6 0/5 417
    101 AAMVtALRKL 6.6 0/1 2/6 0/2 418
    368 VNSCcIPTKL 6.16 0/1 2/6 0/2 419
    13 CLLLIAAGWL 6 - - - 420
    354 VVNQyRMRGL 6 0/1 0/6 - 421
    150 DGLAsSRVRL 6 0/1 2/6 0/2 422
    293 GLECdGRTNL 6 0/1 0/6 423
    330 NYCEgSCPAY 6 0/1 1/6 0/1 424
    176 ASLWIYLKLL 6 0/1 1/6 0/1 425
    212 WNMVeKRVDL 6 1/1 426
    74 FLEAvKRHIL 6 0/1 2/6 0/2 427
    331 YCEGsCPAYL 6 0/1 1/6 0/1 428
    77 AVKRhILSRL 5.6 0/1 1/6 0/1 429
    175 QASLwLYLKL 5.28 0/1 2/6 0/2 430
    326 GYYGnYCEGS 5 0/1 1/6 0/1 431
    159 LYTFiSNEGN 5 0/1 4/6 0/4 432
    327 YYGNyCEGSC 5 0/1 1/6 0/1 433
    [Table 6C]
    HLA-A*0202 binding 9-mer peptides derived from INHBB
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    177 SLWLYLKLL 407.808 0/1 0/8 140
    14 LLLLAAGWL 96.074 - - - 141
    170 NLFVVQASL 79.041 0/1 0/8 142
    213 NMVEKRVDL 63.256 0/1 0/8 143
    172 FWQASLWL 47.291 0/1 0/8 144
    306 QQFFIDFRL 46.48 0/1 0/8 145
    281 RLGDSRHRI 42.774 0/1 0/8 146
    174 VQASLWLYL 34.427 0/1 0/8 147
    257 ELAVVPVFV 28.69 0/1 1/8 0/1 148
    313 RLIGWNDWI 28.116 0/1 1/8 0/1 149
    139 RVSEIISFA 22.546 0/1 3/8 0/3 150
    151 GLASSRVRL 21.362 0/1 0/8 151
    8 ALGAACLLL 21.362 0/1 1/8 0/1 152
    250 VQCDSCQEL 15.096 0/1 1/8 0/1 153
    [Table 6D]
    HLA-A*0201 binding 10-mer peptides derived from INHBB
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    179 WLYLKLLPYV 12951.1 0/1 1/8 0/1 154
    301 NLCCRQQFFI 332.806 0/1 0/8 155
    237 ALFERGERRL 64.814 0/1 0/8 156
    382 MLYFDDEYNI 56.754 0/1 0/8 157
    13 CLLLLAAGWL 56.514 - - - 158
    8 ALGAACLLLL 49.134 - - - 159
    313 RLIGWNDWII 32.081 0/1 0/8 160
    173 VVQASLWLYL 29.711 0/1 2/8 0/2 161
    256 QELAVVPVFV 27.521 0/1 0/8 162
    162 FISNEGNQNL 13.512 0/1 1/8 0/1 163
    305 RQQFFIDFRL 12.562 0/1 0/8 164
    362 GLNPGTVNSC 11.426 0/1 0/7 165
    85 RLQMRGRPNI 10.433 0/1 1/8 0/1 166
    69 RVDGDFLEAV 10.425 0/1 0/8 167
    [Table 7A]
    HLA-A*2402 binding 9-mer peptides derived from KIF20A
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    308 IYNELLYDL 432 0/2 0/14 - 168
    621 MYEEKLNIL 432 0/2 0/14 - 169
    67 VYLRVRPLL 420 0/2 0/14 - 170
    499 KFSAIASQL 56 0/2 0/14 - 171
    304 SFFEIYNEL 44.352 0/2 0/14 - 172
    187 IFNSLQGQL 36 0/2 0/14 - 173
    305 FFEIYNELL 30 1/2 174
    23 MFESTAADL 30 0/2 0/14 - 175
    256 SFDSGIAGL 20 0/2 0/14 - 176
    298 RFSIWISFF 20 - - - 177
    383 IFSIRILHL 20 1/2 178
    647 KIEELEALL 17.28 0/2 0/14 - 179
    625 KLNILKESL 14.4 0/2 0/14 - 180
    695 KLQQCKAEL 13.2 0/2 0/14 - 181
    726 FTIDVDKKL 11.088 0/2 0/14 - 182
    688 QLQEVKAKL 11.088 0/2 0/14 - 183
    [Table 7B]
    HLA-A*2402 binding 10-mer peptides derived from KIF20A
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    308 IYNEILYDLL 432 0/2 0/14 - 184
    182 RSLAlIFNSL 24.192 0/2 1/14 0/1 185
    304 SFFEiYNELL 24 1/2 186
    742 RLLRtELQKL 15.84 0/2 0/14 - 187
    739 KNIRILRTEL 15.84 0/2 0/14 - 188
    218 RQEEmKKLSL 14.4 0/2 2/14 0/2 189
    70 RVRPILPSEL 12.672 0/2 0/14 - 190
    871 RILRsRRSPL 12 0/2 0/14 - 191
    89 RIENvETLVL 12 0/2 1/14 0/1 192
    364 KNQSfASTHL 12 0/2 0/14 - 193
    66 KVYLrVRPLL 11.2 1/2 194
    60 DSMEkVKVYL 10.08 0/2 0/14 - 195
    [Table 8A]
    HLA-A*2402 binding 9-mer peptides derived from KNTC2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    309 KYQAYMSNL 600 1/3 196
    457 VYVPLKELL 432 0/3 0/18 - 197
    414 EYHKLARKL 264 0/3 0/18 - 198
    139 SYELPDTKF 165 0/3 0/18 - 199
    629 KYEKKATLI 150 0/3 0/18 - 200
    400 KYE1RGKEAI 100 0/3 1/18 0/1 201
    124 DFLKIFTFL 50.4 1/3 202
    134 GFLCPSYEL 33 0/3 0/18 - 203
    257 LFNVDAFKL 33 0/3 0/18 - 204
    242 SFDEMNAEL 26.4 0/3 0/18 - 205
    128 IFTFLYGFL 24 0/3 0/18 - 206
    146 KFEEEVPRI 18 0/3 1/18 0/1 207
    368 RINHERNEL 15.84 0/3 1/18 0/1 208
    235 SFMSGADSF 15 0/3 0/18 - 209
    154 IFKDLGYPF 14.4 1/3 210
    563 EYQLWQTT 12.6 0/3 0/18 - 211
    474 KALNKKMGL 12 0/3 1/18 0/1 212
    150 EVPRIFKDL 10.08 1/3 213
    [Table 8B]
    HLA-A*2402 binding 10-mer peptides derived from KNTC2
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    452 KYRAQVYVPL 560 2/3 214
    610 EYEECMSEDL 360 0/3 1/18 0/1 215
    360 KYSVADIERI 100 0/3 0/18 - 216
    227 DYTIKCYESF 100 1/3 217
    146 KFEEEVPRIF 50.4 0/3 0/18 - 218
    90 AFIQQCIRQL 30 0/3 0/18 - 219
    20 RSQDVNKQGL 17.28 0/3 1/18 0/1 220
    501 RTLKEEVQKL, 15.84 0/3 0/18 - 221
    403 RGKEAIETQL 13.44 0/3 1/18 0/1 222
    273 ItALNEQIARL 12 1/3 223
    563 EYQLWQTTT 10.5 0/3 3/22 0/3 224
    467 ETEEEINKAL 10.08 0/3 1/22 0/1 225
    541 LLESTVNQGL 10.08 0/3 1/22 0/1 226
    [Table 9A]
    HLA-A*0201 binding 9-mer peptides derived from TTK
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    462 YMSCFRTPV 878.055 1/1 227
    547 KQIYAIKYV 312.218 1/1 228
    630 NMLEAVHTI 262.897 0/1 1/8 0/1 229
    278 LLNSPDCDV 118.238 0/1 1/8 0/1 230
    498 ILATPLQNL 83.527 0/1 0/8 - 231
    811 YVLGQLVGL 73.172 0/1 0/8 - 232
    719 SLGCILYYM 62.845 1/2 233
    670 QMQPDTTSV 50.232 0/1 0/8 - 234
    804 GTTEEMKYV 50.102 0/1 0/8 - 235
    654 LIVDGMLKL 47.088 0/1 1/8 0/1 236
    363 SLLAKLEET 31.074 0/1 0/8 - 237
    790 YVQIQTHPV 27.995 0/1 0/8 - 238
    785 LLAHPYVQI 26.604 0/1 0/8 - 239
    86 KLIGRYSQA 26.082 0/1 0/8 - 240
    186 NLNLQKKQL 21.362 0/1 0/8 - 241
    671 MQPDTTSVV 20.152 0/1 0/8 - 242
    577 KLQQHSDKI 17.892 0/1 0/8 - 243
    142 FAFVHISFA 14.856 0/1 0/8 - 244
    322 CELRNLKSV 11.509 0/1 0/8 - 245
    824 SILKAAKTL 10.868 0/1 0/8 - 246
    [Table 9B]
    HLA-A*0201 binding 10-mer peptides derived from TTK
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    68 LLLKLEKNSV 437.482 0/1 0/8 - 247
    277 NLLNSPDCDV 257.342 0/1 0/8 - 248
    653 FLIVDGMLKL 226.014 0/1 0/8 - 249
    423 TTFEQPVFSV 195.487 0/1 0/8 - 250
    542 VLNEKKQIYA 190.448 0/1 0/8 - 251
    658 GMLKLIDFGI 161.697 0/1 0/8 - 252
    194 LLSEEEKKNL 148.896 0/1 0/8 - 253
    462 YMSCFRTPW 94.738 1/1 254
    57 MMANNPEDWL 70.685 0/1 0/8 - 255
    600 MVMECGNmL 48.205 0/1 0/8 - 256
    689 YMPPEAIKDM 37.961 0/1 0/8 - 257
    86 KLIGRYSQAI 36.515 0/1 0/8 - 258
    669 NQMQPDTTSV 26.092 0/1 1/8 0/1 259
    497 QILATPLQNL 24.997 0/1 0/8 - 260
    654 LIVDGMLKLI 22.997 0/1 0/8 - 261
    1$6 NLNLQKKQLL 21.362 0/1 1/8 0/1 262
    670 QMQPDTTSVV 20.595 0/1 0/8 - 263
    803 KGTTEEMKYV 20.102 0/1 0/8 - 264
    11 LTIDSIMNKV 15.486 0/1 0/8 - 265
    577 KLQQHSDKII 14.971 0/1 0/8 - 266
    [Table 10A]
    HLA-A*0201 binding 9-mer peptides derived from URICl0
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    131 KIFPRFFMV 1364.78 0/1 0/8 - 267
    204 CLWLAILLL 407.808 0/1 0/8 - 268
    65 LLVVALPRV 271.948 0/1 0/8 - 269
    60 ALLALLLW 242.674 - - - 270
    206 WLAILLLLA 52.561 1/1 271
    212 LLASIAAGL 36.316 1/1 272
    210 LLLLASIAA 31.249 0/1 0/8 - 273
    137 FMVAKQCSA 16.505 0/1 2/8 0/2 274
    58 TMALLALLL 15.428 0/1 2/8 0/2 275
    59 MALLALLLV 13.975 0/1 2/8 0/2 276
    209 ILLLLASIA 12.812 0/1 0/8 - 434
    208 AILLLLASI 12.208 - - - 277
    69 ALPRVWTDA 8.446 0/1 0/8 - 278
    197 SMGESCGGL 8.223 0/1 0/8 - 279
    61 LLALLLVVA 7.964 - - - 280
    67 WALPRVWT 6.097 0/1 0/8 - 281
    72 RVWTDANLT 5.412 0/1 0/8 - 282
    160 FLLEEPMPF 5.2 0/1 1/8 0/1 283
    62 LALLLVVAL 4.292 0/1 0/8 - 284
    57 GTMALLALL 2.525 0/1 1/8 0/1 285
    [Table 10B]
    HLA-A*0201 binding 10-mer peptides derived from URLCl0
    strat position sequence Binding Score positive donor number positive well number positive CTL line SEQ ID NO
    64 LLLVVALPRV 1006.21 0/1 0/8 - 286
    204 GLWLAILLLL 407.808 0/1 1/8 0/1 287
    211 LLLASIAAGL 134.369 1/1 288
    258 TMALLALLLV 115.534 - - - 289
    61 LLALLLWAL 83.527 - - - 290
    160 FLLEEPMPFF 65.782 0/1 0/8 - 291
    209 ILLLLASIAA 31.249 0/1 0/8 - 292
    131 KIFPRFFMVA 26.186 0/1 0/8 - 293
    60 ALLALLLVVA 17.334 - - - 294
    66 LVVALPRVWT 6.097 0/1 0/8 - 295
    59 MALLALLLVV 5.73 - - - 296
    2 RLQRPRQAPA 4.968 0/1 1/8 0/1 297
    112 CQNPRRCKWT 4.156 0/1 0/8 - 298
    72 RVWTDANLTA 3.608 0/1 0/8 - 299
    53 WAPLGTMALL 3.139 0/1 0/8 - 300
    121 TEPYCVIAAV 3.111 0/1 0/8 - 301
    162 LEEPMPFFYL 2.739 0/1 1/8 0/1 302
    181 LEGPPINSSV 2295 0/1 2/8 0/2 303
    170 YLKCCKIRYC 2.024 0/1 0/8 - 304
    130 VKIFPRFFMV 1.81 0/1 0/8 - 305
    • Stimulation of the T cells using the predicted petatides from CDH3 restricted with HLA-A*2402 and establishment for CTL lines stimulated with CDH3 derived peptides
  • CTLs for those peptides derived from CDH3 were generated according to the protocols set forth in "Materials and Methods" section above. Resulting that CTLs having detectable specific CTL activity, as determined by IFN-gamma ELISPOT assay, are shown in Figure 1. In particular, CDH3-A24-9-513 (SEQ ID NO: 19), CDH3-A24-9-406 (SEQ ID NO: 22), CDH3-A24-10-807 (SEQ ID NO: 30), CDH3-A24-10-332 (SEQ ID NO: 34). CDH3-A24-10-655 (SEQ ID NO: 344) and CDH3-A24-10-470 (SEQ ID NO: 358) demonstrated potent IFN-gamma production as compared to the control by IFN-gamma ELISPOT assay, and the cells in the positive well number #5 stimultaed with SEQ ID NO: 19, #2 with SEQ ID NO: 22, #5 with SEQ ID NO: 30, #4 with SEQ ID NO: 34, #1 with SEQ ID NO: 344 and #4 with SEQ ID NO: 358 were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 1. While, other peptides shown in table 2 could not establish the CTL lines despite possible binding activity with HLA-A*2402. For example, the tipical negative peptide (CDH3-A24-30-248) were shown in Figure 1a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with CDH3 derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from CDH3-A24-10-807 (SEQ ID NO: 30) #5 and CDH3-A24-10-655 (SEQ ID NO: 344) #1 CTL line are shown in Figure 1f and g. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing CDH3 and HLA-A*2402
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing CDH3 and HLA-A*2402. Specific CTL activity against COS7 transfected with both full length CDH3 gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express CDH3 and HLA-A*2402, was tested using as effector cells the CTL lines raised by CDH3-A24-10-807 (SEQ ID NO: 30) and CDH3-A24-10-655 (SEQ ID NO: 344). COS7 transfected with full length CDH3 but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length CDH3 were prepared as controls. The CTL clones demonstrating the highest specific CTL activity against COS7 was that transfected with both CDH3 and HLA-A2402 (Figure If and g).
  • These results clearly demonstrate that CDH3-A24-10-807 (SEQ ID NO: 30) and CDH3-A24-10-655 (SEQ ID NO: 344) are naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting CDH3 expressed tumors.
    • Stimulation of the T cells using the predicted peptides fromEPHA4 restricted with HLA-A*2402 or HLA-A*0201. and establishment for CTL lines stimulated with EPHA4 derived peptides
  • CTLs for those peptides derived from EphA4 were generated by IFN-gamma ELISPOT assay. Resulting that CTLs having detectable specific CTL activity , as determined by IFN-gamma ELISPOT assay, are shown in Figure 2. In particular, EphA4-A24-9-453 (SEQ ID NO: 41), EphA4-A24-9-S (SEQ ID NO: 44), EphA4-A24-9-869 (SEQ ID NO: 46), EphA4-A24-9-420 (SEQ ID NO: 48), EphA4-A24-10-24 (SEQ ID NO: 78), EphA4-A02-9-501 (SEQ ID NO: 376) and EphA4-A02-9-165 (SEQ ID NO: 379) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #3 stimultaed with EphA4-A24-9-453 (SEQ ID NO: 41), #2 with EphA4-A24-9-5 (SEQ ID NO: 44), #5 with EphA4-A24-9-869 (SEQ ID NO: 46), #6 with EphA4-A24-9-420 (SEQ ID NO: 48), #4 with EphA4-A24-10-24 (SEQ ID NO: 78), #8 with EphA4-A02-9-501 (SEQ ID NO: 376) and #3 with EphA4-A02-9-165 (SEQ ID NO: 379) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Especcially, CTL lines stimulated with EphA4-A02-9-501 (SEQ ID NO: 376) and EphA4-A02-9-165 (SEQ ID NO: 379) were tested by 51Cr-release assay according to the protocols set forth in the "Materials and Methods" section above. Results are shown in Figure 2a-h. While, other peptides shown in table 3 could not establish the CTL lines despite possible binding activity with HLA-A*2402 or HLA-A*0201. For example, the tipical negative peptide (EphA4-A24-9-384) were shown in Figure 2a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputides.
    • Stimulation of the T cells using the predicted peptides from ECT2 restricted with HLA-A*2402, and establishment for CTL lines stimulated with ECT2 derived peptides
  • CTLs for those peptides derived from ECT2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 3,. In particular, ECT2-A24-9-515 (SEQ ID NO: 80), ECT2-A24-10-40 (SEQ ID NO: 100) and ECT2-A24-10-101 (SEQ ID NO: 101) showed potent IFN-gamma production, and the cells in the positive well number #7 stimultaed with ECT2-A24-9-515 (SEQ ID NO: 80), #2 with ECT2-A24-10-40 (SEQ ID NO: 100) and #1 with ECT2-A24-10-101 (SEQ ID NO: 101) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 3a-d. While, other peptides shown in table 4 could not establish the CTL lines despite possible binding activity with HLA-A*2402. For example, the tipical negative peptide (ECT2-A24-10-322, ECT2-A24-9-657 and ECT2-A24-10-811) were shown in Figure 2a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with ECT2 derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from ECT2-A24-10-40 (SEQ ID NO: 100) #2 CTL line are shown in Figure3c. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing ECT2 and HLA-A*2402
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing ECT2 and HLA-A*2402. Specific CTL activity against COS7 transfected with both full length ECT2 gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express ECT2 and HLA-A*2402, was tested using as effector cells the CTL clone raised by ECT2-A24-10-40 (SEQ ID NO: 100) and the CTL line raised by ECT2-A24-10-101 (SEQ ID NO: 101). COS7 transfected with full length ECT2 but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length ECT2 (replaced other gene e.g. URLC10 or INHBB) were prepared as controls. The CTL line demonstrating the highest specific CTL activity against COS7 was that transfected with both ECT2 and HLA-A2402 (Figure 3c and d).
  • These results clearly demonstrate that ECT2-A24-10-40 (SEQ ID NO: 100) and ECT2-A24-10-101 (SEQ ID NO: 101) are naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting ECT2 expressed tumors.
    • Cytotoxic activity against cancer cell line endogenously expressing HLA-A*2402 and ECT2
  • Furthermore, Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above. As a result, as shown in Fig. 3b, CTL clone stimulated with ECT2-A24-9-515 (SEQ ID NO: 80) showed remarkably high cytotoxic effect towards HLA-A24-positive and ECT-positive cancer cell lines TE6, compared to that towards HLA-A24-negative and ECT-positive cancer cell lines TE5.
    • Stimulation of the T cells using the predicted peptides from HIG2 restricted with HLA-A*2402 or HLA-A*0201, and establishment for CTL lines stimulated with HIG2 derived peptides
  • CTLs for those peptides derived from HIG2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 4. In particular, HIG2-A24-9-19 (SEQ ID NO: 110), HIG2-A24-9-22 (SEQ ID NO: 111), HIG2-A24-9-8 (SEQ ID NO: 387), HIG2-A24-10-7 (SEQ ID NO: 112), HIG2-A24-10-18 (SEQ ID NO: 394), HIG2-A02-9-8 (SEQ ID NO: 114), HIG2-A02-9-15 (SEQ ID NO: 116), HIG2-A02-9-4 (SEQ ID NO: 117) and HIG2-A02-10-8 (SEQ ID NO: 121) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #6 stimultaed with HIG2-A24-9-19 (SEQ ID NO: 110), #7 with HIG2-A24-9-22 (SEQ ID NO: 111), #5 with HIG2-A24-9-8 (SEQ ID NO: 387), #1 with HIG2-A24-10-7 (SEQ ID NO: 112), #7 with HIG2-A24-10-18 (SEQ ID NO: 394), #10 with HIG2-A02-9-8 (SEQ ID NO: 114), #10 with H1G2-A02-9-15 (SEQ ID NO: 116), #10 with HIG2-A02-9-4 (SEQ ID NO: 117) and #9 with HIG2-A02-10-8 (SEQ ID NO: 121) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 4a-j. While, other peptides shown in table 5 could not establish the CTL lines despite possible binding activity with HLA-A*2402. For example, the tipical negative peptide (HIG2-A24-9-7) were shown in Figure 4a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with HIG2 derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from HIG2-A24-9-22 (SEQ ID NO: 111) #7 CTL line, HTG2-A24-9-8 (SEQ ID NO: 387) #5 CTL line, HIG2-A2-4-10-7 (SEQ ID NO: 112) #1 CTL line, HIG2-A24-10-18 (SEQ ID NO: 394) #7 CTL line and HIG2-A02-9-4 (SEQ ID NO: 117) #10 CTL line are shown in Figure 4c, e, f, g and i. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expression HIG2 and HLA-A*0201
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing HIG2 and HLA-A*0201. Specific CTL activity against 293T or COS7 transfected with both full length HIG2 gene and the HLA-A*0201 molecule, which serves as a specific model for the target cells endogenously express HIG2 and HLA-A*0201, was tested using as effector cells the CTL lines raised by HIG2-A02-9-8 (SEQ ID NO: 114), HIG2-A02-9-15 (SEQ ID NO: 116) and the CTL clone raised by HIG2-A02-9-4 (SEQ ID NO: 117). 293T or COS7 transfected with full length ECT2 but not HLA-A*0201 and 293T or COS7 transfected with HLA-A*0201 but not full length ECT2 (or replaced other gene e.g. FoxP3 or TTK) were prepared as controls. The CTL line demonstrating the highest specific CTL activity against 293T or COS7 was that transfected with both ECT2 and HLA-A*0201 (Figure 4e, h and i).
  • These results clearly demonstrate that HIG2-A02-9-8 (SEQ ID NO: 114), HIG2-A02-9-15 (SEQ ID NO: 116) and HIG2-A02-9-4 (SEQ ID NO: 117) are naturally expressed on the target cell surface with HLA-A2402 or HLA-A0201 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting HIG2 expressed tumors.
    • Cytotoxic activity against cancer cell line endogenously expressing HLA-A*Q201 and HIG2
  • Furthermore, Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above. As a result, as shown in Fig. 4i, CTL clone stimulated with HIG2-A02-9-4 (SEQ ID NO: 117) showed remarkably high cytotoxic effect towards HLA-A02-positive and ECT-positive cancer cell lines A498, compared to that towards HLA-A02-negative and ECT-positive cancer cell lines CAki-1.
    • Stimulation of the T cells using, the predicted peptides from INHBB restricted with HLA-A*2402 or HLA-A*0201, and establishment for CTL lines stimulated with INHBB derived peptides,
  • CTLs for those peptides derived from INHBB were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 5. In particular, TNHBB-A24-9-180 (SEQ ID NO: 395), INHBB-A24-10-180 (SEQ ID NO: 133), INHBB-A24-10-305 (SEQ ID NO: 135), INHBB-A24-10-7 (SEQ ID NO: 137) and INHBB-A24-10-212 (SEQ ID NO: 426) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #7 stimultaed with INHBB-A24-9-180 (SEQ ID NO: 395), #3 with INHBB-A24-10-180 (SEQ ID NO: 133), #2 with INHBB-A24-10-305 (SEQ ID NO: 135), #8 and #2 with INHBB-A24-10-7 (SEQ ID NO: 137) and #1 with INHBB-A24-10-212 (SEQ ID NO: 426) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 5b-e. While, other peptides shown in table 6 could not establish the CTL lines despite possible binding activity with HLA-A*2402 and HLA*0201. For example, the tipical negative peptide (INHBB-A24-9-238) were shown in Figure 5a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with INHBB derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from INHBB-A24-9-180 (SEQ ID NO: 395) #7 CTL line, and INHBB-A24-10-305 (SEQ ID NO: 135) #2 CTL line are shown in Figure 5b and d. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing INHBB and HLA-A*2402
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing INHBB and HLA-A*2402. Specific CTL activity against 293T transfected with both full length INHBB gene and the HLA-A*2402 molecule, which serves as a specific model for the target cells endogenously express INHBB and HLA-A*2402, was tested using as effector cells the CTL lines raised by INHBB-A24-10-180 (SEQ ID NO: 133) and INHBB-A24-10-7 (SEQ ID NO: 137) and the CTL clone raised by INHBB-A24-10-305 (SEQ ID NO: 135),. 293T transfected with full length INHBB but not HLA-A*2402 and 293T transfected with HLA-A*2402 but not full length INHBB were prepared as controls. The CTL line demonstrating the highest specific CTL activity against 293T was that transfected with both INHBB and HLA-A*2402 (Figure 5c, d and e).
  • These results clearly demonstrate that INHBB-A24-10-305 (SEQ ID NO: 135), INHBB-A24-10-180 (SEQ ID NO: 133) and INHBB-A24-10-7 (SEQ ID NO: 137) are naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting INHBB expressed tumors.
    • Cytotoxic activity against cancer cell line endogenously expressing HLA-A*2402 and INHBB
  • Furthermore, Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above. As a result, as shown in Fig. 5b, CTL clone stimulated with INHBB-A24-9-180 (SEQ ID NO: 395) showed remarkably high cytotoxic effect towards HLA-A24-positive and INHBB - positive cancer cell lines MIAPaca2, compared to that towards HLA-A24-negative and INHBB -positive cancer cell lines CAki-2.
    • Stimulation of the T cells using the predicted peptides from KIF20A restricted with HLA-A*2402, and establishment for CTL lines stimulated with KIF20A derived peptides
  • CTLs for those peptides derived from KIF20A were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 6. In particular, KIF20A-A24-9-305 (SEQ ID NO: 174), KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-304 (SEQ ID NO: 186) and KIF20A-A24-10-66 (SEQ ID NO: 194) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #2 stimultaed with KIF20A-A24-9-305 (SEQ ID NO: 174), #3 with KIF20A-A24-9-383 (SEQ ID NO: 178), #5 with KIF20A-A24-10-304 (SEQ ID NO: 186) and #6 with KIF20A-A24-10-66 (SEQ ID NO: 194) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 6a-e. While, other peptides shown in table 7 could not establish the CTL lines despite possible binding activity with HLA-A*2402. For example, the tipical negative peptide (KIF20A -A24-9-647 and KIF20A - A24-10-182) were shown in Figure 6a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with KIF20A derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from KIF20A-A24-9-305 (SEQ ID NO: 174) #2 CTL line, KIF20A-A24-10-304 (SEQ ID NO: 186) #5 CTL line and KIF20A-A24-10-66 (SEQ ID NO: 194) #6 CTL line are shown in Figure 6b, d and e. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing KIF20A and HLA-A*2402
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing KIF20A and HLA-A*2402. Specific CTL activity against COS7 transfected with both full length KIF20A gene and the HLA-A*2402 molecule and A24-LCL transfected by electropolation with full length KIF20A gene, which serve as a specific model for the target cells endogenously express KIF20A and HLA-A*2402, was tested using as effector cells the CTL lines raised by KIF20A-A24-9-383 (SEQ ID NO: 178) and KIF20A-A24-10-304 (SEQ ID NO: 186) and the CTL clone raised by KIF20A-A24-10-66 (SEQ ID NO: 194). COS7 transfected with full length KIF20A but not HLA-A*2402 and COS7 transfected with HLA-A*2402 but not full length KIF20A (or replaced full length URLC10 gene), COS7 transfected with HLA-A*2-402 and pulesd with KIF20A-10-308, and A24-LCL transfected with mock vector were prepared as controls. The CTL line demonstrating the highest specific CTL activity against COS7 was that transfected with both KIF20A and HLA-A*2402 (Figure 6b, c and d). Alternatively, the CTL line stuimurated with KIF20A-A24-10-304 (SEQ ID NO: 186) demonstrated against A24-LCL transfected with KIF20A.
  • These results clearly demonstrate that KIF20A-A24-9-383 (SEQ ID NO: 178), KIF20A-A24-10-304 (SEQ ID NO: 186) and KIF20A-A24-10-66 (SEQ ID NO: 194) is naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting KIF20A expressed tumors.
    • Cytotoxic activity against cancer cell line endogenously expressing HLA-A*2402 and KIF20A
  • Furthermore, Cytotoxic activity was performed by cytotoxicity assay according to the protocols set forth in the "Materials and Methods" section above. As a result, as shown in Fig. 6b and e, CTL clone stimulated with KIF20A-A24-9-305 (SEQ ID NO: 174) or KIF20A-A24-10-304 (SEQ ID NO: 186) showed remarkably high cytotoxic effect towards HLA-A24-positive and KIF20A-positive cancer cell lines PK45P or MIAPaca2 respectively, compared to that towards HLA-A24-negative and KIF20A-positive cancer cell lines PK59.
    • Stimulation of the T cells using: the predicted peptides from KNTC2 restricted with HLA-A*2402, and establishment for CTL lines stimulated with KNTC2 derived peptides
  • CTLs for those peptides derived from KNTC2 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 7. In particular, KNTC2-A24-9-309 (SEQ ID NO: 196), KNTC2-A24-9-124 (SEQ ID NO: 202), KNTC2-A24-9-154 (SEQ ID NO: 210), KNTC2-A24-9-150 (SEQ ID NO: 213), KNTC2-A24-10-452 (SEQ ID NO: 214), KNTC2-A24-10-227 (SEQ ID NO: 217) and KNTC2-A24-10-273 (SEQ ID NO: 223) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #8 stimultaed with KNTC2-A24-9-309 (SEQ ID NO: 196), #5 with KNTC2-A24-9-124 (SEQ ID NO: 202), #5 with KNTC2-A24-9-154 (SEQ ID NO: 210), #7 with KNTC2-A24-9-150 (SEQ ID NO: 213), #4 and #5 with KNTC2-A24-10-452 (SEQ ID NO: 214), #1 with KNTC2-A24-10-227 (SEQ ID NO: 217) and #8 with KNTC2-A24-10-273 (SEQ ID NO: 223) were expanded and CTL lines were established. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. Results are shown in Figure 7a-h. While, other peptides shown in table 8 could not establish the CTL lines despite possible binding activity with HLA-A*2402. For example, the tipical negative peptide (KNTC2-A24-10-610) were shown in Figure 7a. In this invention, the peptides which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with KNTC2 derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from KNTC2-A24-9-154 (SEQ ID NO: 210) #5 CTL line and KNTC2-A24-10-452 (SEQ ID NO: 214) #5 CTL line are shown in Figure 7d and f. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing KNTC2 and HLA-A*2402
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells expressing KNTC2 and HLA-A*2402. Specific CTL activity against HEK293 transfected with both full length KNTC2 gene and the HLA-A*2402 molecule which serves as a specific model for the target cells endogenously express KNTC2 and HLA-A*2402, was tested using as effector cells the CTL clones raised by KNTC2-A24-10-452 (SEQ ID NO: 214). HEK293 transfected with full length KNTC2 but not HLA-A*2402, HEK293 transfected with HLA-A*2402 but not full length KNTC2 and HEK293 transfected with HLA-A*2402 and pulesd with KNTC2-9-309 were prepared as controls. The CTL line demonstrating the highest specific CTL activity against HEK293 was that transfected with both KNTC2 and HLA-A*2402 (Figure 7f).
  • These results clearly demonstrate that KNTC2-A24-1O-452 (SEQ ID NO: 214) is naturally expressed on the target cell surface with HLA-A2402 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting KNTC2 expressed tumors.
    • Stimulation of the T cells using the predicted peptides from TTK restricted with HLA-A*0201, and establishment for CTL lines stimulated with TTK derived peptides
  • CTLs for those peptides derived from TTK were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by an IFN-gamma ELISPOT assay are shown in Figure 8. As depicted in Figure 8b-d, TTK-A2-9-462 (SEQ ID NO: 227), TTK-A2-9-547 (SEQ ID NO: 228), TTK-A2-9-719 (SEQ ID NO: 233) and TTK-A2-10-462 (SEQ ID NO: 254) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #4 stimultaed with TTK-A2-9-462 (SEQ ID NO: 227), #2 with TTK-A2-9-547 (SEQ ID NO: 228), #1 with TTK-A2-9-719 (SEQ ID NO: 233) and #8 with TTK-A2-10-462 (SEQ ID NO: 254) were expanded. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. While, other peptides shown in table 9 could not establish the CTL lines despite possible binding activity with HLA-A*0201. For example, the tipical negative peptide (TTK-A2-9-278) were shown in Figure 8a. In this invention, the peptiedes which could establish CTL line were selected as potent CTL stimulation peputide.
    • Establishment for CTL clones stimulated with TTK derived peptides
  • Furthermore, the limiting dilution from these CTL lines was performed according to the protocols set forth in the "Materials and Methods" section above. The establishment of CTL clones from TTK-A2-9-462 (SEQ ID NO: 227) #4 CTL line, TTK-A2-9-547 (SEQ ID NO: 228) #2 CTL line, TTK-A2-9-719 (SEQ ID NO: 233) #1 CTL line and TTK-A2-10-462 (SEQ ID NO: 254) #8 CTL line were shown in Figure 8d, c, d and e. CTL clones had potent and specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse.
    • Specific CTL activity against the target cells expressing TTK and HLA-A*0201
  • The established CTL clone raised against these peptides were examined for their ability to recognize the target cells endogenously expressing TTK and HLA-A*0201. Specific CTL activity against COS7 transfected with both the full length TTK gene and the HLA-A*0201 molecule, which is a specific model for the target cells endogenously express TTK and HLA-A*0201, was tested using as effector cells the CTL clones raised by TTK-A2-9-462 (SEQ ID NO: 227), TTK-A02-9-547 (SEQ ID NO: 228), TTK-A2-9-719 (SEQ ID NO: 233) and TTK-A2-10-462 (SEQ ID NO: 254). COS7 transfected with full length TTK but HLA-A*0201, COS7 transfected HLA-A*0201 but not full length of TTK (or replaced full length HIG2 gene) and COS7 transfected with HLA-A*0201 and pulsed with different target epitope peputide, were prepared as controls. The CTL Clone having the highest specific CTL activity against COS7 was that transfected with both TTK and HLA-A*0201 (Figure 8b, c, d and e).
  • These results clearly demonstrate that TTK-A2-9-462 (SEQ ID NO: 227), TTK-A02-9-547 (SEQ ID NO: 228), TTK-A2-9-719 (SEQ ID NO: 233) and TTK-A02-10-462 (SEQ ID NO: 254) are naturally expressed on the target cell surface with HLA-A2 molecule and recognize CTL. Furthermore, these peptides are epitope peptides, which may serve as cancer vaccines targeting TTK expressed tumors.
    • Stimulation of the T cells using the predicted peptides from URLC10 restricted with HLA-A*0201, and establishment for CTL lines stimulated with URLC10 derived peptides
  • CTLs for those peptides derived from URLC10 were generated according to the protocols set forth in the "Materials and Methods" section above. Resulting CTLs having detectable specific CTL activity as determined by IFN-gamma ELISPOT assay are shown in Figure 9. As shown in Figure 9b-d, URLC-A2-9-206 (SEQ ID NO: 271), URLC-A2-9-212 (SEQ ID NO: 272) and URLC-A2-10-211 (SEQ ID NO: 288) demonstrated potent IFN-gamma production by IFN-gamma ELISPOT assay, and the cells in the positive well number #7 stimultaed with URLC-A2-9-206 (SEQ ID NO: 271), #3 with URLC-A2-9-212 (SEQ ID NO: 272) and #5 with URLC-A2-10-211 (SEQ ID NO: 288) were expanded. Those CTL lines having higher specific CTL activities against the peptide-pulsed target as compared to the activities against target without peptide pulse were determined by ELISA. While, other peptides shown in table 10 could not establish the CTL lines despite possible binding activity with HLA-A*0201. For example, the tipical negative peptide (URLC-A2-9-58) were shown in Figure 9a. In this invention, the peptiede which could establish CTL line were selected as potent CTL stimulation peputide.
    • Specific CTL activity against the target cells expressing URLC10 and HLA-A*0201
  • The established CTL line raised against these peptides were examined for their ability to recognize the target cells endogenously expressing URLC10 and HLA-A*0201. Specific CTL activity against COS7, Hek293 and 293T transfected with both full length URLC10 gene and the HLA-A*0201 molecule, which serves as a specific model for the target cells endogenously express URLC10 and HLA-A*0201, was tested using as effector cells the CTL line raised by URLC10-A02-10-211. COS7, Hek293 or 293T transfected with full length URLC10 but not HLA-A*0201 (replaced HLA-A*2402), COS7, Hek293 or 293T transfected with HLA-A*0201 but not full length URLC10 and COS7 transfected with HLA-A*0201 and pulsed with different target epitope peputide (URLC10-A02-10-64) were prepared as controls. The CTL line demonstrating the highest specific CTL activity against COS7, Hek293 or 293T was that transfected with both URLC10 and HLA-A*0201 (Figure 9-2).
  • These results clearly demonstrate that URLC10-A02-10-211 is naturally expressed on the target cell surface with HLA-A*0201 molecule and recognizes CTL. Furthermore, this peptide was epitope peptides, which may utilize cancer vaccine targeting URLC10 expressed tumors.
    • Homology analysts of the antigen peptides
  • The CTL clones established against the following peptides showed potent specific CTL activity.
    • CDH3-A24-9-513 (SEQ ID NO: 19),
    • CDH3-A24-9-406 (SEQ ID NO: 22),
    • CDH3-A24-10-807 (SEQ ID NO: 30),
    • CDH3-A24-10-332 (SEQ ID NO: 34),
    • CDH3-A24-10-655 (SEQ ID NO: 344),
    • CDH3-A24-10-470 (SEQ ID NO: 358),
    • EphA4-A24-9-453 (SEQ ID NO: 41),
    • EphA4-A24-9-5 (SEQ ID NO: 44),
    • EphA4-A24-9-869 (SEQ ID NO: 46),
    • EphA4-A24-9-420 (SEQ ID NO: 48),
    • EphA4-A24-10-24 (SEQ ID NO: 78),
    • EphA4-A02-9-501 (SEQ ID NO: 376),
    • EphA4-A02-9-165 (SEQ ID NO: 379),
    • ECT2-A24-9-515 (SEQ ID NO: 80),
    • ECT2-A24-10-40 (SEQ ID NO: 100),
    • ECT2-A24-10-101 (SEQ ID NO: 101),
    • HIG2-A24-9-19 (SEQ ID NO: 110),
    • HIG2-A24-9-22 (SEQ ID NO: 111),
    • HIG2-A24-9-8 (SEQ ID NO: 387),
    • HIG2-A24-10-7 (SEQ ID NO: 112),
    • HIG2-A24-10-18 (SEQ ID NO: 394),
    • HIG2-A02-9-8 (SEQ ID NO: 114),
    • HIG2-A02-9-15 (SEQ ID NO: 116),
    • HIG2-A02-9-4 (SEQ ID NO: 117),
    • HIG2-A02-10-8 (SEQ ID NO: 121),
    • INHBB-A24-9-180 (SEQ ID NO: 395),
    • INHBB-A24-10-180 (SEQ ID NO: 133),
    • INHBB-A24-10-305 (SEQ ID NO: 135),
    • INHBB-A24-10-7 (SEQ ID NO: 137),
    • INHBB-A24-10-212 (SEQ ID NO: 426),
    • KIF20A-A24-9-305 (SEQ ID NO: 174),
    • KIF20A-A24-9-383 (SEQ ID NO: 178),
    • KIF20A-A24-10-304 (SEQ ID NO: 186),
    • KIF20A-A24-10-66 (SEQ ID NO: 194),
    • KNTC2-A24-9-309 (SEQ ID NO: 196),
    • KNTC2-A24-9-124 (SEQ ID NO: 202),
    • KNTC2-A24-9-154 (SEQ ID NO: 210),
    • KNTC2-A24-9-150 (SEQ ID NO: 213),
    • KNTC2-A24-10-452 (SEQ ID NO: 214),
    • KNTC2-A24-10-227 (SEQ ID NO: 217),
    • KNTC2-A24-10-273 (SEQ ID NO: 223),
    • TTK-A02-9-462 (SEQ ID NO: 227),
    • TTK-A02-9-547 (SEQ ID NO: 228),
    • TTK-A02-9-719 (SEQ ID NO: 233),
    • TTK-A02-10-462 (SEQ ID NO: 254),
    • URLC-A02-9-206 (SEQ ID NO: 271),
    • URLC-A02-9-212 (SEQ ID NO: 272) and
    • URLC-A02-14-211 (SEQ ID NO: 288)
  • This suggests that the sequences of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 are homologous to the peptides derived from other molecules, which are known to sensitize human immune system.
  • To exclude this possibility, homology analysis was performed with the peptide sequences as queries using BLAST algorithm
    (http://www.ncbi.nlm.nih.gov/blast/blast.cgi). No significant sequence homology was revealed.
    These results suggest that the sequences of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 376, 379, 80, 100, 101, 110, 111, 387, 112, 394, 114, 116, 117, 121, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217, 223, 227, 228, 233, 254, 271, 272 or 288 are unique and thus possess a low risk of raising unintended immunologic response to any unrelated molecule.
    • DISCUSSION
  • Identification of new TAAs, particularly those that induce potent and specific anti-tumor immune responses, warrants further development of the clinical application of peptide vaccination strategies in various types of cancer (Boon T. et al., (1996) J Exp Med 183: 725-9.; van der Bruggen P et al., (1991) Science 254: 1643-7.; Brichard V et al., (1993) J Exp Med 178: 489-95.; Kawakami Y et al., (1994) J Exp Med 180: 347-52.; Shichijo S et al., (1998) J Exp Med 187:277-88.; Chen YT et al., (1997) Proc.Natl.Acd. Sci. USA, 94: 1914-8.; Harris CC., (1996) J Natl Cancer Inst 88:1442-5.; Butterfield LH et al., (1999) Cancer Res 59:3134-42.; Vissers JL et al., (1999) Cancer Res 59: 5554-9.; van der Burg SH et al., (1996) J. Immunol 156:3308-14.; Tanaka F et al., (1997) Cancer Res 57:4465-8.; Fujie T et al., (1999) Int J Cancer 80:169-72.; Kikuchi M et al., (1999) Int J Cancer 81 : 459-66.; Oiso M et al., (1999) Int J Cancer 81:387-94.).
  • cDNA microarray technologies can disclose comprehensive profiles of gene expression of malignant cells (Lin YM, et al., Oncogene. 2002 .; Kitahara O, et al., Cancer Res. 2001 May 1;61:3544-9.; Suzuki C, et al., Cancer Res. 2003 .; Ashida S, Cancer Res. 2004 .; Ochi K, et al., Int J Oncol. 2004 Mar;24(3):647-55.; Kaneta Y, et al., Int J Oncol. 2003 Sep;23:681-91.; Obama K, Hepatology. 2005 Jun;41:1339-48.; Kato T, et al., Cancer Res. 2005 .; Kitahara O, et al., Neoplasia. 2002 Jul-Aug;4:295-303.; Saito-Hisaminato A et al., DNA Res 2002, 9: 35-45.) and, find utility in the identification of potential TAAs. Among the transcripts that are up-regulated in various cancers, novel human genes, termed CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10, were identified using these technologies.
  • As demonstrated above, CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10, are over-expressed in various cancers but show minimal expression in normal tissues. In addition, these genes have been shown to have a significant function related to cell proliferation. Thus, peptides derived from CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 can serve as TAA epitopes, which, in turn, can be used to induce significant and specific immune responses against cancer cells.
  • Thus, as CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and URLC10 are novel TAAs, vaccines using these epitope peptides find utility as immunotherapeutics against various carcinomas or other disease expressing these molecules.
    • Industrial Applicability
  • The present invention identifies new TAAs, particularly those which induce potent and specific anti-tumor immune responses. Such TAAs warrants further development as peptide vaccines against diseases associated with the over-expression of CDH3, EPHA4, ECT2, HIG2, INHBB, KIF20A, KNTC2, TTK and/or, URLC10 e.g. cancers.
  • All patents, patent applications, and publications cited herein are incorporated by reference.
  • While the invention has been described in detail and with reference to specific embodiments thereof, it is to be understood that the foregoing description is exemplary and explanatory in nature and is intended to illustrate the invention and its preferred embodiments. Through routine experimentation, one skilled in the art will readily recognize that various changes and modifications can be made therein without departing from the spirit and scope of the invention. Thus, the invention is intended to be defined not by the above description, but by the following claims and their equivalents.
  • Furthermore, the present invention relates to the following items:
    • An isolated peptide of less than about 15 amino acids selected from the group consisting of peptides comprising the amino acid sequences of SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223. A peptide having cytotoxic T cell inducibility, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 37, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223, wherein 1, 2, or several amino acids are substituted, deleted, or added.
  • The peptide of item 2, wherein the second amino acid from the N-terminus is phenylalanine, tyrosine, methionine, or tryptophan.
  • The peptide of item 2 or 3, wherein the C-terminal amino acid is phenylalanine, leucine, isoleucine, tryptophan, or methionine.
  • An isolated peptide of less than about 15 amino acids selected from the group consisting of peptides comprising the amino acid sequences of SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288.
  • A peptide having cytotoxic T cell inducibility, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 376, 379, 114, 116, 117, 121, 227, 228, 233, 254, 271, 272 or 288, wherein 1, 2, or several amino acids are substituted, deleted, or added.
  • The peptide of item 6, wherein the second amino acid from the N-terminus is leucine or methionine.
  • The peptide of item 6 or 7, wherein the C-terminal amino acid is valine or leucine.
  • A pharmaceutical composition for treating or preventing a disease associated with over-expression of the genes of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 said composition comprising one or more peptides of item 1 to 8 or a polynucleotide encoding the peptides.
  • A pharmaceutical composition of item 9, wherein the disease associating genes of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 include cancers. A pharmaceutical composition of item 10, wherein the cancers include bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • An exosome that presents on its surface a complex comprising a peptide of item 1-4 and an HLA antigen.
  • The exosome of item 12, wherein the HLA antigen is HLA-A24.
  • The exosome of item 13, wherein the HLA antigen is HLA-A2402.
  • An exosome that presents on its surface a complex comprising a peptide of item 5-8 and an HLA antigen.
  • The exosome of item 15, wherein the HLA antigen is HLA-A2.
  • The exosome of item 16, wherein the HLA antigen is HLA-A0201.
  • A method of inducing antigen-presenting cells having a high cytotoxic T cell inducibility comprising the step of contacting an antigen-presenting cell with a peptide of item 1-8.
  • A method of inducing cytotoxic T cells by contacting a T cell with a peptide of item 1-8.
  • A method of inducing antigen-presenting cells having high cytotoxic T cell inducibility, said method comprising the step of transferring a gene comprising a polynucleotide encoding a peptide of item 1 to 8 to an antigen-presenting cell. An isolated cytotoxic T cell, which is induced by contacting a T cell with a peptide of item 1-8 or which is transduced with the nucleic acids encoding the TCR subunits polypeptides binding with a peptide of any one items 1 to 8 in the context of HLA-A24 or HLA-A2.
  • An antigen-presenting cell, which comprises a complex formed between an HLA antigen and a peptide of item 1 to 8.
  • An antigen-presenting cell, induced by the method of item 18-20.
  • A vaccine for inhibiting proliferation of cells expressing genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 wherein the vaccine comprises a peptide of item 1-8 as the active ingredient.
  • The vaccine of item 24, wherein the cells expressing genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 include cancers.
  • The vaccine of item 25, wherein the cancers include bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor. The vaccine of item 26, formulated for administration to a subject whose HLA antigen is HLA-A24 or HLA-A2.
  • A method of treating or preventing a disease associated with over-expression of the genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 in a subject comprising administering to said subject a vaccine comprising a peptide of item 1-8, an immunologically active fragment thereof, or a polynucleotide encoding said peptide or immunologically active fragment.
  • The method of item 28, wherein the disease associating genes of 1, 3, 5, 7, 9, 11, 13, 15 and/or 17 include cancers.
  • A method of treating or preventing cancers of item 29, wherein the cancer cells include bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancer, liver, NSCLC, lymphoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  • A method of screening for a peptide which 1, 2, or several amino acids are substituted, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: SEQ ID NO: 19, 22, 30, 34, 344, 358, 41, 44, 46, 48, 78, 80, 100, 101, 110, 111, 387, 112, 394, 395, 133, 135, 137, 426, 174, 178, 186, 194, 196, 202, 210, 213, 214, 217 or 223, said method comprising the steps of:
    1. (a) comforming no significant sequence homology to the entire sequence of 1, 2 or several amino acids substitute;
    2. (b) measuring the CTL inducibility of the candidate substitute peptide; and
    3. (c) selecting the peptide which CTL inducibility is same to or higher than the original peptide.
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Claims (17)

  1. A peptide of less than about 15 amino acids having cytotoxic T cell inducibility, wherein said peptide comprises the amino acid sequence selected from the group consisting of:
    (a) an amino acid sequence selected from the group consisting of SEQ ID NOs: 174, 178, 186 and 194; and
    (b) an amino acid sequence selected from the group consisting of SEQ ID NOs: 174, 178, 186 and 194, in which 1, 2, or several amino acids are substituted, deleted, or added,
    wherein said peptides possess an ability to induces cytotoxic T lymphocytes specific to KIF20A.
  2. The peptide of claim 1, wherein the second amino acid from the N-terminus is phenylalanine, tyrosine, methionine, or tryptophan, and/or the C-terminal amino acid is phenylalanine, leucine, isoleucine, tryptophan, or methionine.
  3. A pharmaceutical composition for use in treating or preventing a disease associated with over-expression of the gene of SEQ ID NO: 11 said composition comprising at least one peptide of claim 1 or 2 or a polynucleotide encoding the peptide.
  4. An exosome that presents on its surface a complex comprising a peptide of claim 1 or 2 and an HLA antigen.
  5. The exosome of claim 4, wherein the HLA antigen is HLA-A24.
  6. The exosome of claim 5, wherein the HLA antigen is HLA-A2402.
  7. An in vitro method of inducing an antigen-presenting cell having a high cytotoxic T cell inducibility comprising the step of:
    (a) contacting an antigen-presenting cell with a peptide of claim 1 or 2, or
    (b) transferring a gene comprising a polynucleotide encoding a peptide of claim 1 or 2 to an antigen-presenting cell.
  8. An in vitro method of inducing a CTL, comprising the steps of:
    (i) contacting an antigen-presenting cell with a peptide of claim 1 or 2, and
    (ii) mixing the antigen-presenting cell of step (i) with a CD8+ T cell and co-culturing.
  9. An isolated cytotoxic T cell, which is induced by the method of claim 8 or which is transduced with the nucleic acids encoding the TCR subunits polypeptides binding with a peptide of claim 1 or 2 in the context of HLA-A24.
  10. An antigen-presenting cell, which comprises a complex formed between an HLA antigen and a peptide of claim 1 or 2, or induced by the method of claim 7.
  11. A vaccine for use in inhibiting proliferation of cells expressing a gene of SEQ ID NO: 11, wherein the vaccine comprises at least one peptide of claim 1 or 2 as the active ingredient.
  12. The vaccine of claim 11, wherein the cells expressing a gene of SEQ ID NO: 11 are cancer cells.
  13. A peptide of claim 1 or 2, an immunologically active fragment thereof, or a polynucleotide encoding said peptide or immunologically active fragment for use in treating or preventing a disease associated with over-expression of the gene of SEQ ID NO: 11.
  14. The pharmaceutical composition of claim 3 or the peptide or polynucleotide of claim 13, wherein the disease associated with over-expression of the gene of SEQ ID NO: 11 is cancer.
  15. The vaccine of claim 12, or the pharmaceutical composition or the peptide or polynucleotide of claim 14, wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, CML, colorectal cancer, endometriosis, esophageal cancer, gastric cancer, diffused type gastric cancel, liver cancer, NSCLC, lymphoma, osteosarcoma ovarian cancer, pancreatic cancer prostate cancer, renal carcinoma, SCLC, soft tissue tumor and testicular tumor.
  16. The vaccine of claim 15, formulated for administration to a subj ect whose HLA antigen is HLA-A24.
  17. A method of identifying for a peptide having an ability to induce CTL against cells expressing KIF20A, wherein said method comprises the steps of:
    (i) providing or generating at least one candidate sequence which consists of an amino acid sequence modified by substituting, deleting, or adding one, two or several amino acid residues to an original amino acid sequence, wherein the original amino acid sequence is selected from the group consisting of SEQ ID NOs: 174, 178, 186 and 194;
    (ii) selecting the candidate sequence that does not have substantial significant homology with the peptides derived from any known human gene products other than said tumor-associated antigens;
    (iii) contacting a peptide consisting of the candidate sequence selected in step (ii) with antigen presenting cells;
    (iv) contacting the antigen presenting cells of step (iii) with T-cells to evaluate the ability of the peptide to stimulate the T-cells; and
    (v) identifying the peptide of which CTL inducibility is same to or higher than a peptide consisting of the original amino acid sequence.
EP12175487.3A 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 174, 178, 186 or 194 for cancers expressing tumor-associated antigens Withdrawn EP2573110A3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US90294907P 2007-02-21 2007-02-21
EP12151717.1A EP2476694B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP08710443.6A EP2121731B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
EP08710443.6 Division 2008-02-21
EP12151717.1A Division-Into EP2476694B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151717.1 Division 2012-01-19

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EP12151714.8A Active EP2476693B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing HIG2 tumor-associated antigens
EP12151720A Withdrawn EP2465866A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175490.7A Withdrawn EP2574623A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151717.1A Active EP2476694B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151723A Withdrawn EP2476695A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151719.7A Not-in-force EP2465865B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175481.6A Withdrawn EP2570428A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 379 or 376 for cancers expressing tumor-associated antigens
EP12175487.3A Withdrawn EP2573110A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 174, 178, 186 or 194 for cancers expressing tumor-associated antigens
EP12175489.9A Withdrawn EP2594582A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 110, 111, 387, 112, 394 114, 116, 117 or 121 for cancers expressing tumor-associated antigens
EP12175488.1A Withdrawn EP2583976A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with SEQ ID NO: 210, 196, 202, 213, 214, 214 or 223 for cancers expressing tumor-associated antigens
EP12151705.6A Not-in-force EP2476692B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175480.8A Withdrawn EP2567971A3 (en) 2007-02-21 2008-02-21 Peptide vaccines comprising Seq Id 80, 100 or 101 for cancers expressing tumor-associated antigens
EP12175484.0A Withdrawn EP2594581A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 178, 174, 186 or 194 for cancers expressing tumor-associated antigens
EP12151722.1A Active EP2465867B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151711.4A Not-in-force EP2465864B1 (en) 2007-02-21 2008-02-21 EphA4 derived peptide vaccines
EP12175483.2A Withdrawn EP2570430A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 78, 41, 44, 46 or 48 for cancers expressing tumor-associated antigens
EP12175479.0A Withdrawn EP2573109A3 (en) 2007-02-21 2008-02-21 Peptide vaccines comprising Seq Id 101, 80 or 100 for cancers expressing tumor-associated antigens
EP12175482.4A Withdrawn EP2570429A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 48, 41, 44, 46 or 78 for cancers expressing tumor-associated antigens
EP12175478A Withdrawn EP2565203A1 (en) 2007-02-21 2008-02-21 Peptide vaccines having Seq ID: 344v for cancers expressing tumor-associated antigens
EP08710443.6A Active EP2121731B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens

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EP12151714.8A Active EP2476693B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing HIG2 tumor-associated antigens
EP12151720A Withdrawn EP2465866A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175490.7A Withdrawn EP2574623A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151717.1A Active EP2476694B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151723A Withdrawn EP2476695A3 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151719.7A Not-in-force EP2465865B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175481.6A Withdrawn EP2570428A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 379 or 376 for cancers expressing tumor-associated antigens

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EP12175489.9A Withdrawn EP2594582A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 110, 111, 387, 112, 394 114, 116, 117 or 121 for cancers expressing tumor-associated antigens
EP12175488.1A Withdrawn EP2583976A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with SEQ ID NO: 210, 196, 202, 213, 214, 214 or 223 for cancers expressing tumor-associated antigens
EP12151705.6A Not-in-force EP2476692B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12175480.8A Withdrawn EP2567971A3 (en) 2007-02-21 2008-02-21 Peptide vaccines comprising Seq Id 80, 100 or 101 for cancers expressing tumor-associated antigens
EP12175484.0A Withdrawn EP2594581A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq Id No: 178, 174, 186 or 194 for cancers expressing tumor-associated antigens
EP12151722.1A Active EP2465867B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens
EP12151711.4A Not-in-force EP2465864B1 (en) 2007-02-21 2008-02-21 EphA4 derived peptide vaccines
EP12175483.2A Withdrawn EP2570430A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 78, 41, 44, 46 or 48 for cancers expressing tumor-associated antigens
EP12175479.0A Withdrawn EP2573109A3 (en) 2007-02-21 2008-02-21 Peptide vaccines comprising Seq Id 101, 80 or 100 for cancers expressing tumor-associated antigens
EP12175482.4A Withdrawn EP2570429A3 (en) 2007-02-21 2008-02-21 Peptide vaccines with Seq ID No: 48, 41, 44, 46 or 78 for cancers expressing tumor-associated antigens
EP12175478A Withdrawn EP2565203A1 (en) 2007-02-21 2008-02-21 Peptide vaccines having Seq ID: 344v for cancers expressing tumor-associated antigens
EP08710443.6A Active EP2121731B1 (en) 2007-02-21 2008-02-21 Peptide vaccines for cancers expressing tumor-associated antigens

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