EP2534250B1 - Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase - Google Patents

Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase Download PDF

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Publication number
EP2534250B1
EP2534250B1 EP11710314.3A EP11710314A EP2534250B1 EP 2534250 B1 EP2534250 B1 EP 2534250B1 EP 11710314 A EP11710314 A EP 11710314A EP 2534250 B1 EP2534250 B1 EP 2534250B1
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EP
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Prior art keywords
seq
nadh
gogat
wheat
plant
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Not-in-force
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EP11710314.3A
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German (de)
English (en)
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EP2534250A2 (fr
Inventor
Jérôme Salse
Umar Masood Quraishi
Caroline Pont
Florent Murat
Jacques Le Gouis
Stéphane Lafarge
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Genoplante Valor SAS
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Genoplante Valor SAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cereal-Derived Products (AREA)

Claims (17)

  1. Procédé pour améliorer le remplissage des grains d'une plante de blé, ledit procédé comprenant la surexpression dans ladite plante de blé d'un glutamate synthase NADH-dépendante (NADH-GoGAT) présentant au moins 95 % d'identité avec le polypeptide de séquence SEQ ID NO : 1, le pourcentage d'identité entre deux séquences étant calculé à partir d'un alignement des deux séquences sur leur longueur entière.
  2. Procédé selon la revendication 1, dans lequel le remplissage des grains est amélioré par l'augmentation du poids des grains et/ou de la teneur en protéines des grains.
  3. Procédé selon la revendication 1 ou la revendication 2, dans lequel ladite NADH-GoGAT a la séquence d'acides aminés SEQ ID NO : 22.
  4. Procédé selon l'une quelconque des revendications 1 à 3, dans lequel ladite NADH-GoGAT est codée par une séquence nucléotidique choisie dans le groupe constitué de SEQ ID NO : 2, SEQ ID NO : 3, SEQ ID NO : 4, SEQ ID NO : 5, SEQ ID NO : 23, SEQ ID NO : 24, SEQ ID NO : 25 et SEQ ID NO : 26, de préférence SEQ ID NO : 23, SEQ ID NO : 24, SEQ ID NO : 25 et SEQ ID NO : 26.
  5. Cassette d'expression recombinante, qui comprend un polynucléotide codant pour une NADH-GoGAT telle que définie dans l'une quelconque des revendications 1, 3 ou 4, sous le contrôle d'un promoteur hétérologue fonctionnel dans une cellule végétale.
  6. Vecteur recombinant, qui contient une cassette d'expression comprenant un polynucléotide codant pour une NADH-GoGAT telle que définie dans l'une quelconque des revendications 1, 3 ou 4, sous le contrôle d'un promoteur.
  7. Cellule hôte, qui contient une cassette d'expression recombinante selon la revendication 5 ou un vecteur recombinant selon la revendication 6.
  8. Cellule hôte selon la revendication 7 qui est une cellule de plante de blé.
  9. Procédé de production d'une plante de blé transgénique, présentant un remplissage des grains amélioré, ledit procédé comprenant :
    - la fourniture d'une cellule de plante de blé selon la revendication 8 ;
    - la régénération à partir de ladite cellule de plante de blé d'une plante de blé transgénique surexprimant une NADH-GoGAT telle que définie dans l'une quelconque des revendications 1 ou 3.
  10. Plante de blé transgénique pouvant être obtenue par le procédé selon la revendication 9, ladite plante de blé transgénique contenant une cassette d'expression recombinante telle que définie dans la revendication 5.
  11. Plante de blé transgénique ou l'un de ses organes ou tissus isolé, qui comprend, intégrée de façon stable dans son génome, une cassette d'expression recombinante comprenant un polynucléotide codant pour une NADH-GoGAT telle que définie dans l'une quelconque des revendications 1, 3 ou 4.
  12. Graines de blé, qui contiennent une cassette d'expression recombinante selon la revendication 5 obtenues à partir d'une plante de blé transgénique selon l'une quelconque des revendications 10 ou 11.
  13. Protéine glutamate synthase NADH-dépendante de blé isolée présentant au moins 95 % d'identité avec le polypeptide de séquence SEQ ID NO : 1, le pourcentage d'identité entre deux séquences étant calculé à partir d'un alignement des deux séquences sur leur longueur entière.
  14. Protéine glutamate synthase NADH-dépendante de blé isolée selon la revendication 13, qui a la séquence d'acides aminés SEQ ID NO : 22.
  15. Polynucléotide isolé choisi dans le groupe constitué de :
    a) un polynucléotide codant pour une NADH-GoGAT de blé, lequel polypeptide présentant au moins 95 % d'identité avec le polypeptide de séquence SEQ ID NO : 1, le pourcentage d'identité entre deux séquences étant calculé à partir d'un alignement des deux séquences sur leur longueur entière ;
    b) un polynucléotide complémentaire du polynucléotide a).
  16. Polynucléotide isolé selon la revendication 15, qui est choisi dans le groupe constitué de : SEQ ID NO : 2, SEQ ID NO : 3, SEQ ID NO : 4, SEQ ID NO : 5, SEQ ID NO : 23, SEQ ID NO : 24, SEQ ID NO : 25 et SEQ ID NO : 26, de préférence SEQ ID NO : 23, SEQ ID NO : 24, SEQ ID NO : 25 et SEQ ID NO : 26.
  17. Paire d'amorces, qui est choisie dans le groupe constitué des séquences SEQ ID NO : 7 et SEQ ID NO : 8, SEQ ID NO : 9 et SEQ ID NO : 10, SEQ ID NO : 11 et SEQ ID NO : 12, SEQ ID NO : 13 et SEQ ID NO : 14, SEQ ID NO : 15 et SEQ ID NO : 16, SEQ ID NO : 17 et SEQ ID NO : 18, et SEQ ID NO : 19 et SEQ ID NO : 20, SEQ ID NO : 27 et SEQ ID NO : 28, SEQ ID NO : 29 et SEQ ID NO : 30, SEQ ID NO : 31 et SEQ ID NO : 32, SEQ ID NO : 33 et SEQ ID NO : 34, de préférence SEQ ID NO : 27 et SEQ ID NO : 28, SEQ ID NO : 29 et SEQ ID NO : 30, SEQ ID NO : 31 et SEQ ID NO : 32, SEQ ID NO : 33 et SEQ ID NO : 34.
EP11710314.3A 2010-02-08 2011-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase Not-in-force EP2534250B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11710314.3A EP2534250B1 (fr) 2010-02-08 2011-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10290058A EP2354232A1 (fr) 2010-02-08 2010-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la NADH-glutamate synthase
PCT/IB2011/050534 WO2011095958A2 (fr) 2010-02-08 2011-02-08 Amélioration du remplissage du grain d'une plante par modulation de l'activité nadh-glutamate synthase
EP11710314.3A EP2534250B1 (fr) 2010-02-08 2011-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase

Publications (2)

Publication Number Publication Date
EP2534250A2 EP2534250A2 (fr) 2012-12-19
EP2534250B1 true EP2534250B1 (fr) 2017-04-12

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EP10290058A Withdrawn EP2354232A1 (fr) 2010-02-08 2010-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la NADH-glutamate synthase
EP11710314.3A Not-in-force EP2534250B1 (fr) 2010-02-08 2011-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la nadh-glutamate synthase

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EP10290058A Withdrawn EP2354232A1 (fr) 2010-02-08 2010-02-08 Amélioration portant sur le remplissage des grains de blé via la modulation de l'activité de la NADH-glutamate synthase

Country Status (4)

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US (1) US20130047300A1 (fr)
EP (2) EP2354232A1 (fr)
CA (1) CA2787688A1 (fr)
WO (1) WO2011095958A2 (fr)

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CN108570517B (zh) * 2018-06-12 2021-10-01 江苏省农业科学院 与弱筋小麦宁麦9号低蛋白相关的特异性引物及其应用
CN108841840B (zh) * 2018-07-09 2022-05-10 中国科学院遗传与发育生物学研究所 蛋白TaNADH-GoGAT在调控植物产量中的应用

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US5283323A (en) 1985-08-07 1994-02-01 The United States Of America As Represented By The Department Of Health And Human Services Method of producing immune response
US5190065A (en) 1991-11-29 1993-03-02 International Business Machines Corporation Self-contained washing unit for printed circuit boards
DE60024607T2 (de) 1999-04-19 2006-08-10 Biogemma Pflanzentransformationsverfahren
US20110131679A2 (en) * 2000-04-19 2011-06-02 Thomas La Rosa Rice Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement
AU2002314417A1 (en) * 2001-06-22 2003-01-08 Syngenta Participations Ag Identification and characterization of plant genes
WO2004070039A2 (fr) 2003-02-04 2004-08-19 Cropdesign N.V. Promoteurs de riz
CN101173000A (zh) * 2006-10-30 2008-05-07 中国科学院上海生命科学研究院 作物籽粒灌浆基因gif1及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HARUSHIMA YOSHIAKI ET AL: "A high-density rice genetic linkage map with 2275 markers using a single F2 population", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 148, no. 1, 1 January 1998 (1998-01-01), pages 479 - 494, XP009134467, ISSN: 0016-6731 *
SALSE JEROME ET AL: "Identification and characterization of shared duplications between rice and wheat provide new insight into grass genome evolution", PLANT CELL, vol. 20, no. 1, January 2008 (2008-01-01), pages 11 - 24, ISSN: 1040-4651 *

Also Published As

Publication number Publication date
US20130047300A1 (en) 2013-02-21
WO2011095958A3 (fr) 2011-11-24
WO2011095958A2 (fr) 2011-08-11
AU2011212138A1 (en) 2012-08-16
EP2354232A1 (fr) 2011-08-10
CA2787688A1 (fr) 2011-08-11
EP2534250A2 (fr) 2012-12-19

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