EP2380665A1 - Wiederverschließbarer Deckel für Reaktionsgefäße - Google Patents

Wiederverschließbarer Deckel für Reaktionsgefäße Download PDF

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Publication number
EP2380665A1
EP2380665A1 EP10160322A EP10160322A EP2380665A1 EP 2380665 A1 EP2380665 A1 EP 2380665A1 EP 10160322 A EP10160322 A EP 10160322A EP 10160322 A EP10160322 A EP 10160322A EP 2380665 A1 EP2380665 A1 EP 2380665A1
Authority
EP
European Patent Office
Prior art keywords
reclosable
vessels
cap
caps
arrangement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10160322A
Other languages
English (en)
French (fr)
Inventor
Hubert Schuldlos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to EP10160322A priority Critical patent/EP2380665A1/de
Publication of EP2380665A1 publication Critical patent/EP2380665A1/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • the first approach is an automated high throughout approach for multiplexing using devices for parallel processing, whereas the second approach is a manual approach sufficient for low throughput.
  • the state of the art provides at least two different setups, namely microtiter plates or an arrangement of single vessels. If the parallel processing of the liquids is not desired for the entire workflow and a separation is required at some point in time, the use of an arrangement of single vessels is suitable. Here, it is reasonable to arrange the plurality of vessel with a spacing that is equal to the spacing of the wells within microtiter plates such that standard liquid handling devices using multi pipetting heads are applicable.
  • Micronic (Lelystad, The Netherlands) offers solutions to seal all vessels of an arrangement at a time (e.g. TPE Capmat to seal 96 wells or tubes at a time). These sealings of Micronic even allow the separation of the vessels afterwards ( TPE Capcluster ), but the sealings are not suitable for convenient reclosing.
  • Eppendorf tubes In the field of manual fluid handling the well known Eppendorf tubes exist that allow for multiple reclosing ( Eppendorf AG, Hamburg Germany). On the other hand, these Eppendorf tubes are not suitable for multiplexing using pipetting robots, because the cap of the tube in open state will hinder the accessibility of neighboring vessels.
  • One aspect of the present invention is a reclosable cap (1) to be applied to vessels (7) arranged in a microtiter plate format, said reclosable cap comprises
  • the reclosable caps of the present invention can be applied to vessels after the parallel liquid handling within the plurality of vessels is finalized and it is desired to temporary close said vessels.
  • the closed vessel can be separated and further processed individually.
  • some of the vessels can be stored in a refrigerator, whereas other are used immediately. Since the caps of the present invention are reclosable, it is also possible to withdraw only parts of the liquid form a respective vessel and reclose the cap for subsequent storage.
  • the reclosable caps for vessels in a multiwell arrangement of the present invention provide a significant advantage when compared with the state of the art closures for this purpose, namely the cap is fixed to the vessel even in the open state. This reduces the risk of contaminations that may occur compared to state of the art solutions using e.g. screwed caps, because these state of the art closure need to be placed temporarily e.g. on a bench prior to reclosing the vessel.
  • sealing foils are not reclosable and caps with a permanently fixed cap are not suitable for parallel, automated handling.
  • Another aspect of the present invention is an arrangement of reclosable caps (8) according to the present invention, whereas a plurality of reclosable caps are linked to each other via a plurality of connectors (9) to form a grid, the spacing of said grid is adapted such that each reclosable cap is capable of closing a vessel of a respective arrangement of vessels.
  • Yet another aspect of the present invention is a method to handle a plurality of liquids, said method comprises the steps
  • reclosable caps of the present invention are especially useful in a workflow comprising automated high throughput steps as well as manual individual steps.
  • One aspect of the present invention is a reclosable cap (1) to be applied to vessels (7) arranged in a microtiter plate format, said reclosable cap comprises
  • the reclosable caps of the present invention comprise two components linked to each other in such a way that both components are movable relative to each other.
  • There are several ways to set up such a cap namely a set up with a single piece or a set up comprising two or three pieces. In all cases the link between the two components need to be flexible in order to move them relative to each other.
  • a preferred link between the two components is e.g. a hinge.
  • a set up comprising at least two pieces offers the opportunity to use different material for the components and the link.
  • a preferable embodiment of the reclosable cap is e.g. that a rigid plastic material is used for the components and another flexible material for the link.
  • a preferred reclosable cap according to the present invention is a cap, whereas said removable cap is integrally formed.
  • Another preferred reclosable cap according to the present invention is a cap, whereas said removable cap is integrally formed from one material.
  • Yet another preferred reclosable cap according to the present invention is a cap, whereas said removable cap is integrally molded.
  • Still another preferred reclosable cap according to the present invention is a cap, whereas said removable cap is integrally molded from plastic material.
  • plastic disposables are well known technique that provides for fast and cheap production.
  • such disposables are producible by milling, too.
  • the reclosable caps of the present invention are designed to be used for liquid handling workflows as part of biological or chemical reactions that should be performed multiple times, e.g. in screening applications to test a plurality of compounds with respect to a certain property. Consequently, the material should be resistant to certain chemicals.
  • a suitable material can be chosen from state of the art known to the person skilled in the art depending on the liquids to be used (material should be resistant to chemicals), the desired workflow (material should be stable regarding temperature and/or mechanical stress, transparent to light for analysis) or the material of the respective vessels (contact of the materials should be water- or air-tight).
  • a preferred reclosable cap according to the present invention is a cap, whereas said vessels (7) are reaction vessels.
  • Another preferred reclosable cap according to the present invention is a cap, whereas said vessels (7) are sample containers or storage vessels.
  • the reclosable caps of the present invention are designed to be used to temporarily close vessels that are arranged in a multiwell format.
  • Said multiwell formats are defined via the spacing of the wells like in case of the standard 96 well microtiter plates (SBS standard). Additionally, other multiwell plates with defined geometry exist having different number of wells.
  • a preferred reclosable cap according to the present invention is a cap, whereas said vessels (7) are arranged in a 4-, 6-, 8-, 16- or 24- well format, more preferred in a 96-, 384- or 1536- well format.
  • reclosable caps of the present invention are designed for vessels that hold and store liquids and consequently, a preferred reclosable cap according to the present invention is a cap, whereas said closed state with said through hole (3) closed by said second lug (6) is water-tight.
  • the reclosable caps close the vessels in such a way that evaporation is prevented.
  • another preferred reclosable cap according to the present invention is a cap, whereas said closed state with said through hole (3) closed by said second lug (6) is air-tight.
  • a preferred reclosable cap according to the present invention is a cap, whereas said open state of said through hole is configured in such a way that the interior of said vessel is accessible via said through hole by a pipette tip.
  • yet another preferred reclosable cap according to the present invention is a cap, whereas said attaching of said first component (2) to the opening of a vessel (7) is reversible.
  • Another aspect of the present invention is an arrangement of reclosable caps (8) according to the present invention, whereas a plurality of reclosable caps are linked to each other via a plurality of connectors (9) to form a grid, the spacing of said grid is adapted such that each reclosable cap is capable of closing a vessel of a respective arrangement of vessels.
  • Such an arrangement of reclosable caps is preferred to apply a plurality of caps to vessels in one step. It is possible to apply an arrangement of reclosable caps that only closes a certain part of an arrangement of vessels and therefore, to close all vessels of the arrangement more than one arrangement of reclosable caps is necessary. On the other hand, it is possible to apply an arrangement of reclosable caps to an arrangement of vessels such that each vessel is closed by one cap.
  • the reclosable caps of the present invention are designed to be used preferably in workflows having both automated, parallelized as well as manual, individual steps. Consequently, it is preferred that the vessels of the arrangement are separable after the caps are applied and therefore, the linkage between the caps needs to be disconnected at some time.
  • a preferred arrangement of reclosable caps according to the present invention is an arrangement, whereas said connectors (9) are refrangible.
  • a more preferred arrangement of reclosable caps according to the present invention is an arrangement, whereas said connectors (9) each have a predetermined breaking point such that said reclosable caps are separable.
  • the material of the caps and of the connectors is the same, whereas another possibility is to use a different material for the connectors. Since the connectors are preferably refrangible, it is reasonable to use a soft material for the connectors, but to use a solid, chemically resistant material for the caps.
  • reclosable caps Another preferred arrangement of reclosable caps according to the present invention is an arrangement, whereas said arrangement of reclosable caps is integrally formed.
  • Yet another preferred arrangement of reclosable caps according to the present invention is an arrangement, whereas said arrangement of reclosable caps is integrally formed from one material.
  • Still another preferred arrangement of reclosable caps according to the present invention is an arrangement, whereas said arrangement of reclosable caps is integrally molded.
  • Yet another aspect of the present invention is a method to handle a plurality of liquids, said method comprises the steps
  • the method of the present invention is a workflow comprising both parallelized as well as individual processing steps, whereas the vessels can be closed after the parallel processing steps and separated for further individual processing.
  • a preferred method according to the present invention is a method, whereas said vessels are closed in step c) using an arrangement of reclosable caps according to the present invention.
  • a more preferred method according to the present invention is a method, whereas said vessels are separated in step d) by breaking said connectors (9).
  • Another more preferred method according to the present invention is a method, whereas said vessels are separated in step d) by breaking said connectors (9) at predetermined breaking points.
  • the present invention is especially suitable for workflows that have both automated, parallelized as well as manual, individual steps.
  • step b) and step c) are performed using an automated set-up.
  • a more preferred method according to the present invention is a method, whereas step b) is performed simultaneously using a multi pippetting device.
  • step e) is performed using a manual set-up.
  • said method further comprises the step
  • a preferred method according to the present invention is a method, whereas additional steps are performed after step b) and prior to step c), said additional steps are adding additional liquids, mixing said liquids and/or withdrawing part of the mixture.
  • a batch of 96 blood samples is purified in a 96 well format using an automated workflow to provide 96 purified nucleic acids eluates. Afterwards, said eluates need to be divided in order to give different users the possibility to perform a plurality of analysis (e.g. PCR) as well as to store the vessels (e.g. in a freezer). Consequently, it is preferable that the vessels comprising the eluate are closed after the automated nucleic acid purification workflow has been finalized.
  • a plurality of analysis e.g. PCR
  • each vessel is closed using a reclosable cap.
  • the attached reclosable cap can be opened with one hand, whereas liquid can be withdrawn or added using a manual pipette using the other hand.
  • the reclosable caps of the present invention will speed up the workflow, because several hand movements can be avoided. Since the time a vessel is open needs to be as short as possible in order to reduce the risk of contaminations, an increase in workflow speed will be of benefit for this topic as well.
EP10160322A 2010-04-19 2010-04-19 Wiederverschließbarer Deckel für Reaktionsgefäße Withdrawn EP2380665A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10160322A EP2380665A1 (de) 2010-04-19 2010-04-19 Wiederverschließbarer Deckel für Reaktionsgefäße

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP10160322A EP2380665A1 (de) 2010-04-19 2010-04-19 Wiederverschließbarer Deckel für Reaktionsgefäße

Publications (1)

Publication Number Publication Date
EP2380665A1 true EP2380665A1 (de) 2011-10-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP10160322A Withdrawn EP2380665A1 (de) 2010-04-19 2010-04-19 Wiederverschließbarer Deckel für Reaktionsgefäße

Country Status (1)

Country Link
EP (1) EP2380665A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020040987A1 (en) * 2018-08-23 2020-02-27 Becton, Dickinson And Company Blood collection devices, systems, and methods

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5112574A (en) * 1991-04-26 1992-05-12 Imanigation, Ltd. Multititer stopper array for multititer plate or tray
US5683659A (en) * 1995-02-22 1997-11-04 Hovatter; Kenneth R. Integral assembly of microcentrifuge strip tubes and strip caps
EP0834729A2 (de) * 1996-09-26 1998-04-08 Becton, Dickinson and Company Mikrotestplatte und Verfahren zur DNA-Analyse
US6145688A (en) * 1996-07-17 2000-11-14 Smith; James C. Closure device for containers
US6202878B1 (en) * 1998-03-03 2001-03-20 Chromacol Limited Closures
US20060169708A1 (en) * 2004-12-16 2006-08-03 Cepheid Cap for vessel for performing multi-stage process
EP1731443A1 (de) * 2004-03-16 2006-12-13 Prescribe Genomics Co. Behälter mit deckel
US20090011401A1 (en) * 2006-01-13 2009-01-08 Roche Diagnostics Operations, Inc. Group of reagent carriers that is combined to form a composite
WO2010020043A1 (en) * 2008-08-21 2010-02-25 Dna Genotek Inc. Sample receiving device

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5112574A (en) * 1991-04-26 1992-05-12 Imanigation, Ltd. Multititer stopper array for multititer plate or tray
US5683659A (en) * 1995-02-22 1997-11-04 Hovatter; Kenneth R. Integral assembly of microcentrifuge strip tubes and strip caps
US6145688A (en) * 1996-07-17 2000-11-14 Smith; James C. Closure device for containers
EP0834729A2 (de) * 1996-09-26 1998-04-08 Becton, Dickinson and Company Mikrotestplatte und Verfahren zur DNA-Analyse
US6202878B1 (en) * 1998-03-03 2001-03-20 Chromacol Limited Closures
EP1731443A1 (de) * 2004-03-16 2006-12-13 Prescribe Genomics Co. Behälter mit deckel
US20060169708A1 (en) * 2004-12-16 2006-08-03 Cepheid Cap for vessel for performing multi-stage process
US20090011401A1 (en) * 2006-01-13 2009-01-08 Roche Diagnostics Operations, Inc. Group of reagent carriers that is combined to form a composite
WO2010020043A1 (en) * 2008-08-21 2010-02-25 Dna Genotek Inc. Sample receiving device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020040987A1 (en) * 2018-08-23 2020-02-27 Becton, Dickinson And Company Blood collection devices, systems, and methods
JP2021534943A (ja) * 2018-08-23 2021-12-16 ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company 採血装置

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