EP2245160A4 - Cell surface display of proteins - Google Patents

Cell surface display of proteins

Info

Publication number
EP2245160A4
EP2245160A4 EP09703905A EP09703905A EP2245160A4 EP 2245160 A4 EP2245160 A4 EP 2245160A4 EP 09703905 A EP09703905 A EP 09703905A EP 09703905 A EP09703905 A EP 09703905A EP 2245160 A4 EP2245160 A4 EP 2245160A4
Authority
EP
European Patent Office
Prior art keywords
proteins
cell surface
surface display
display
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09703905A
Other languages
German (de)
French (fr)
Other versions
EP2245160A1 (en
Inventor
Heinrich Volschenk
Mariska Lilly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stellenbosch University
Original Assignee
Stellenbosch University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stellenbosch University filed Critical Stellenbosch University
Publication of EP2245160A1 publication Critical patent/EP2245160A1/en
Publication of EP2245160A4 publication Critical patent/EP2245160A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP09703905A 2008-01-22 2009-01-21 Cell surface display of proteins Withdrawn EP2245160A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA200800623 2008-01-22
PCT/IB2009/000086 WO2009093118A1 (en) 2008-01-22 2009-01-21 Cell surface display of proteins

Publications (2)

Publication Number Publication Date
EP2245160A1 EP2245160A1 (en) 2010-11-03
EP2245160A4 true EP2245160A4 (en) 2011-03-30

Family

ID=40900797

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09703905A Withdrawn EP2245160A4 (en) 2008-01-22 2009-01-21 Cell surface display of proteins

Country Status (2)

Country Link
EP (1) EP2245160A4 (en)
WO (1) WO2009093118A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8815553B2 (en) 2008-11-15 2014-08-26 The Regents Of The University Of California Engineering of yeast for cellulosic ethanol production
WO2010096562A2 (en) * 2009-02-20 2010-08-26 Mascoma Corporation Yeast cells expressing an exogenous cellulosome and methods of using the same
DE102010056289A1 (en) 2010-12-24 2012-06-28 Geneart Ag Process for the preparation of reading frame correct fragment libraries
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
WO2014078716A1 (en) * 2012-11-16 2014-05-22 The Regents Of The University Of California Recombinant bacillus subtilis that can grow on plant biomass
WO2016007886A1 (en) * 2014-07-11 2016-01-14 Northwestern University Yeast-based biosensor
CN108676811B (en) * 2018-05-28 2021-07-13 郝志敏 Gene traceless editing vector and application thereof in organism gene editing
CN111961690A (en) * 2020-08-19 2020-11-20 首都师范大学 Method for producing ethanol by batch-type supplementary material simultaneous saccharification and fermentation of pennisetum alopecuroides through steam explosion

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046362A1 (en) * 1998-03-10 1999-09-16 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL111415A0 (en) * 1994-10-27 1994-12-29 Yeda Res & Dev Modified cellulose-binding domain (cbd) proteins and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046362A1 (en) * 1998-03-10 1999-09-16 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BAYER ET AL: "The potential of cellulases and cellulosomes for cellulosic waste management", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 18, no. 3, 8 June 2007 (2007-06-08), pages 237 - 245, XP022110187, ISSN: 0958-1669, DOI: 10.1016/J.COPBIO.2007.04.004 *
FIEROBE H P ET AL: "Design and production of active cellulosome chimeras. Selective incorporation of dockerin-containing enzymes into defined functional complexes", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 276, no. 24, 15 June 2001 (2001-06-15), pages 21257 - 21261, XP002498644, ISSN: 0021-9258, [retrieved on 20010404], DOI: 10.1074/JBC.M102082200 *
FIEROBE H-P ET AL: "Degradation of Cellulose Substrates by Cellulosome Chimeras - SUBSTRATE TARGETING VERSUS PROXIMITY OF ENZYME COMPONENTS", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 277, no. 51, 20 December 2002 (2002-12-20), pages 49621 - 49630, XP002613753, ISSN: 0021-9258, [retrieved on 20021022], DOI: 10.1074/JBC.M207672200 *
LILLY MARISKA ET AL: "Heterologous expression of a Clostridium minicellulosome in Saccharomyces cerevisiae", FEMS YEAST RESEARCH, vol. 9, no. 8, December 2009 (2009-12-01), pages 1236 - 1249, XP002622026 *
See also references of WO2009093118A1 *
VAN DER VAART J MARCEL ET AL: "Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 63, no. 2, 1 February 1997 (1997-02-01), pages 615 - 620, XP009130380, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
EP2245160A1 (en) 2010-11-03
WO2009093118A9 (en) 2009-11-05
WO2009093118A1 (en) 2009-07-30

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Legal Events

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