EP2176196A1 - Inoculum de micro-organisme, procédé et compositions - Google Patents

Inoculum de micro-organisme, procédé et compositions

Info

Publication number
EP2176196A1
EP2176196A1 EP08799583A EP08799583A EP2176196A1 EP 2176196 A1 EP2176196 A1 EP 2176196A1 EP 08799583 A EP08799583 A EP 08799583A EP 08799583 A EP08799583 A EP 08799583A EP 2176196 A1 EP2176196 A1 EP 2176196A1
Authority
EP
European Patent Office
Prior art keywords
spores
concentrate
mycorrhiza
carrier
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08799583A
Other languages
German (de)
English (en)
Other versions
EP2176196A4 (fr
Inventor
Marianne Wilma Venter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocult Pty Ltd
Original Assignee
Biocult Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocult Pty Ltd filed Critical Biocult Pty Ltd
Publication of EP2176196A1 publication Critical patent/EP2176196A1/fr
Publication of EP2176196A4 publication Critical patent/EP2176196A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C05G5/27—Dispersions, e.g. suspensions or emulsions
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/30—Layered or coated, e.g. dust-preventing coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes

Definitions

  • This invention relates to a method of producing an endomycorrhiza inoculum, endomycorrhiza inoculum products produced or producible by such method and methods of using the aforegoing products.
  • VAM fungi vesicular-arbuscular mycorrhiza
  • AM arbuscular mycorrhiza
  • endomycorrhiza fungi vesicular-arbuscular mycorrhiza
  • VAM arbuscular mycorrhiza
  • endomycorrhiza arbuscular mycorrhiza
  • endomycorrhiza on plant roots has a number of beneficial effects on the plant.
  • the hyphae of endomycorrhiza grow into areas the coarser plant roots cannot reach, for example in clay soils, forming what can be described as an extended root system.
  • Phosphate and calcium as well as other minerals and nutrients are absorbed by the hyphae and translocated into the plant roots.
  • the endomycorrhiza receives carbohydrates from the plant. Both are fed by the exchange which is mutually beneficial.
  • the presence of endomycorrhiza on a root system also has the effect of being antagonistic against pathogens and nematodes. Furthermore, endomycorrhiza excrete various growth hormones that stimulate plant growth.
  • Fumigation, cultivation and excessive fertilisation have been found to deplete and/or destroy the endomycorrhiza populations on the plant roots and in the soil. It has, in view of the benefits of endomycorrhiza infection, and the depletion of endomycorrhiza in soil, been proposed that plant root systems should be deliberately infected with endomycorrhiza.
  • a problem in the art is that of ensuring not only that the root system is infected with endomycorrhiza but is infected at a rate such that infection occurs before pathogens and nematodes have a deleterious effect on the plant.
  • a method is known of producing an endomycorrhiza inoculum which comprises growing a host plant in a growing medium which contains endomycorrhiza so that the roots of the host plant become endomycorrhiza-infected and recovering therefrom an inoculum.
  • This method was improved according to South African patent no. 95/10628 by mixing the comminuted roots with all or part of the water with which the roots of the host plant were washed before the roots were comminuted, with a carbon source (e.g. sugar or CO 2 gas), with at least one microbial metabolite, with at least one live organism, with at least one enzyme and with at least one bacterial and fungi culture medium in which live micro-organisms have been grown.
  • a carbon source e.g. sugar or CO 2 gas
  • the inoculum so produced was used successfully, it had a number of shortcomings. It was a heavy, bulky product of which large volumes needed to be used to be effective. The application of the inoculum for inoculating plants was cumbersome. A need has been recognized for an endomycorrhiza inoculum which is more concentrated, can be made available in water-soluble/dispersible form or in a lightweight granular form, or for seed coating, and the manufacture of which lends itself for satisfactory quality control.
  • a method of producing an endomycorrhiza inoculum which comprises growing a host plant in a growing medium which contains endomycorrhiza so that the roots of the host plant become endomycorrhiza-infected and recovering therefrom an inoculum, which further includes removing the host plant, including the roots, from the growth medium and processing the growth medium containing spores and hyphae by washing it thoroughly, e.g.
  • a concentrated mycorrhiza spore powder is preferably combined with the further features of including in said concentrated mycorrhiza-spore powder a carrier dispersing agent, being a substance having dispersing agent properties and being soluble/dispersible in water to hold the mycorrhiza spores in stable suspension.
  • the absorbent carrier powder may itself be or may include this carrier dispersing agent.
  • the growth medium may be a mixture of sand on the one hand, and soil and clay or clay-like matter on the other hand in a suitable ratio, e.g. between 6:1 and 1 :1 by volume, preferably about 3:2.
  • the soil and clay or clay-like matter may contain one or more of zeolite powder, vermiculite, coco peat, peat moss, montmorilonite, bentonite, apatite. Also present may be one or more of perlite, bark, horticultural peat and coir (the fibrous material from coconut husks). If zeolites are used, the preferred pore sizes are in the range of from about 3 to about 10 A in diameter.
  • a suitable example is zeolite clino natural.
  • composition of the growth medium depends also on the pH requirements of the mycorrhiza.
  • Typical examples are 3 parts sand and 2 parts vermiculite and/or peat (by volume).
  • the host plant may, for example, be selected from various grass species, legumes, such as peas, maize, sorghum, medico spp (e.g. medico sativa) or any other plant species sufficiently easy to grow rapidly and suitable as a host plant for the mycorrhiza.
  • legumes such as peas, maize, sorghum, medico spp (e.g. medico sativa) or any other plant species sufficiently easy to grow rapidly and suitable as a host plant for the mycorrhiza.
  • Suitable grass host plants include Sudan grass, which grows best in summer, or rye (Korog), which grows best in winter.
  • the growth period of the host plant may be e.g. 1 to 6 months, preferably 2 to 5 months, e.g. about 3 to 4 months and this growth may be performed under shelter, e.g. in nursery tunnels or greenhouses. For quality control frequent control checks are performed to determine the number of spores in any production batch and thereby determine when the spores are ready for harvesting.
  • Washing of the mycorrhiza-infected growth medium may be performed with water, preferably with the minimum volume of water capable of retrieving substantially the entire mycorrhiza spore content, e.g., in practice, in a total volume of between about 1 and 100 times, preferably from 1 to 30 times, e.g. about twice the volume of growth medium, e.g. divided into a plurality of successive washings. Washing the growth medium is preferably performed by mixing it thoroughly with a portion of the water, allowing heavy sand particles to settle for a few seconds and decanting through a coarse screen, e.g. of from 150 to 300 micron, preferably 180 to 250 micron, e.g. 200 micron, to collect roots and coarse debris, and through one or more finer screens, preferably a plurality of finer screens to capture the spores.
  • a coarse screen e.g. of from 150 to 300 micron, preferably 180 to 250 micron, e.g. 200 micron
  • At least two of the screens are used in the range of between 20 and 90 microns, e.g. selected from the range of 38/45/53/60 microns, for example, one screen 38 to 45 microns and a second screen 53 to 60 microns.
  • the spores may then be washed from these finer screens with a small volume of water into a container, e.g. in a total volume of water equal to from 1 to 20% v/v, preferably from about 3 to 8% v/v, e.g. about 4 to 6% v/v, based on the original volume of growth medium, i.e. about 2% per screen.
  • This volume of water, containing the spores is now taken up in from 5 to 100 times, preferably 6 to 80 times, e.g. about 10 times its weight of fine absorbent carrier powder.
  • the mixture thus obtained is nearly dry (preferably about 10% moisture).
  • the fine absorbent carrier powder may be a fine (10 to 150 micron) zeolite powder and/or one or more clay minerals, e.g. bentonite and/or montmorilonite and/or apatite and/or comminuted perlite.
  • Perlite when used is preferably comminuted by heating, e.g. to about 900 0 C.
  • this mixture is preferably sieved through a sieve, e.g. of from 80 to 200 micron, preferably 100 to 150 micron, most preferably 120 micron, depending on the spore sizes to be allowed to pass. 120 micron was found, in practice, to be the best mesh size to allow all mycorrhiza spores to pass through the sieve.
  • This mixture is now preferably mixed with the aforesaid carrier/dispersing agent, being a substance having dispersing agent properties and being soluble/dispersible in water and capable of holding the mycorrhiza spores in stable suspension. Further preferred properties thereof are that it is wholly organic (and suitable as an ingredient of a composition which is certified "organic"), readily biodegradable, on the one hand inert, on the other hand suitable as a nutrient for micro-organisms and generally advantageous for such micro-organisms as are relevant in the present context.
  • organic carrier is of plant origin, preferably a soya product, e.g. based on a by-product of soya meal manufacture.
  • a preferred absorbent carrier powder and carrier dispersing agent may be of a type described in South African patent 96/6318, the relevant contents of which are to be considered part of the present disclosure, or any organic soya powder product or mixtures of these.
  • ZA Patent 96/6318 describes a microbial carrier comprising a comminuted inorganic silicious mineral, preferably heat- comminuted perlite.
  • mycorrhiza spores are about eight decimal orders of magnitude larger in size than the microbes for which the carrier is described in ZA Patent 96/6318 and for which that carrier had been used in practice in the past.
  • a mycorrhiza concentrate comprising endomycorrhiza spores and a powderous extender and carrier medium which includes a substance serving as a carrier/dispersing agent, and which concentrate contains said spores in a concentration of not less than 700 spores per gram, preferably not less than 1000 spores per gram.
  • the spore content is from 700 to 1300 spores per gram, more preferably from 800 to 1200 spores per gram and most preferably from 1000 to 1100 spores per gram.
  • this concentrate is wholly dispersible in water to form a stable suspension of the mycorrhiza spores.
  • the powderous carrier is selected from any one of the aforegoing powders or a combination of two or more of these and has particle sizes to pass sieve sizes ranging from 20 to 200 micron, preferably from 50 to 150 micron, more preferably from 30 to 120 micron.
  • the carrier/dispersing agent may be as set out above.
  • This final concentrate may now be further converted into a variety of commercial products and be used in a variety of manners.
  • the concentrate may be converted into a water-soluble or dispersible composition
  • a water-soluble or dispersible composition comprising 10 to 80% by weight, preferably 30 to 70% of the concentrate, the balance being one or more concentrates of a variety of growth promoting micro-organisms and/or their growth promoting metabolites, e.g. enzyme(s), more particularly a) Trichoderma fungi spp, e.g. Trichoderma harzianum, e.g. the T22 strain; b) bacteria, preferably nitrogen binding types, e.g.
  • the aforesaid ingredients may be mixed together, bagged or otherwise packaged, and preferably vacuum-sealed or alternatively stored under an inert atmosphere.
  • the aforesaid bacteria are e.g. Actimomycetes thermophilus, Streptomyces spp, Azotobacter chococcum, Mixobacteria celvibrio, M. cytophaga, Bacillus ciculaus, B. subtilis, Fulvoviridis.
  • a suitable enzyme which can be added is the bacterial enzyme known as Cyto-Turf cytozome as available from Mailco Turf, Umhlali, South Africa.
  • a preferred ratio of mixing is, for example, from 50 to 100, e.g. 75 parts by weight Mycorrhiza concentrate, from 70 to 150, e.g. 116 parts by weight Trichoderma concentrate (spore count 1 x 10 9 /g) and from 5 to 15, e.g. 9 parts by weight Azobacter concentrate (spore count 6 x 10 8 /g).
  • the final mix may, for example, contain not less than 375 mycorrhiza spores per gram. This is achieved by careful quality control.
  • Methods of using the water-soluble/dispersible formulation include dissolving/dispersing it in water and applying the dispersion through an irrigation system or for dipping into it the roots of plants or applying it to plants with a watering can or the like. It has been found that the solutions/dispersions of the formulation are stable for prolonged periods without agitation and do not clog irrigation systems.
  • this formulation is dispersed in water in a weight ratio of powder to water of from 1 : 20 to 1 : 100, e.g. about 1 : 50 and this may then be diluted further with water depending on the crop to be irrigated.
  • the mycorrhiza concentrate is converted into granules. It is a particular advantage that the granules can be made of very low bulk density such as from 500 to 800, preferably 550 to 750, more particularly about 600 to 700 g/l of granules.
  • the mycorrhiza spore count of the granules may, for example, be from 1 to 100 spores per gram, e.g. 30 to 40 spores/g.
  • the granules comprise the spores applied onto a solid carrier substance, preferably together with growth-enhancing micro-organisms or substances as described above in the context of the water-soluble/dispersible formulation.
  • the solid carrier substance is preferably biodegradable. Suitable solid carrier substances include farinaceous substances, e.g. starch or rice, although sago is particularly preferred. However, it is also possible to use as solid carrier substance particles sterilised round seeds, e.g. sterilised by boiling, e.g. sorghum seed. The granule size (e.g. the sago or seed size) is selected so that the product can be readily scattered on lands.
  • Preferred embodiments comprise from 100 to 800, preferably 200 to 450, e.g. about 300 parts by weight mycorrhiza concentrate, and from 5000 to 15 000, preferably 7000 to 12 000, e.g. about 9000 parts by weight sago.
  • the preferred embodiments may contain additions of 50 to 150, preferably 70 to 120, e.g. 90 parts by weight Azotobacter and/or from 50 to 250, preferably 80 to 200, e.g. 135 parts by weight Trichoderma spp.
  • the granules may be manufactured by mixing the solid carrier, e.g. sago (or other farinaceous substance), e.g. in a mixer, with the remaining dry ingredients and with moistening, e.g. while turning the mixer. Any suitable dye, preferably a vegetable dye, may be added as well. After thorough mixing, the granulate is packaged, e.g. in bags, and sealed. The granulate may be applied in planting holes with plants placed on top or it may be mixed with seed and sown.
  • the solid carrier e.g. sago (or other farinaceous substance)
  • moistening e.g. while turning the mixer.
  • Any suitable dye preferably a vegetable dye
  • the endomycorrhiza concentrate is incorporated in a seed-coating powder, including a suitable carrier or mixture of carriers and extenders.
  • the seed-coating powder also contains growth-enhancing micro-organisms and/or their metabolites as aforesaid, Trichoderma spp being preferred.
  • the preferred carrier comprises rock phosphate and the carrier/dispersing agent composition referred to above.
  • Preferred proportions are from 3 to 20 parts by weight, preferably 5 to 12 parts by weight, e.g. about 7 parts by weight of mycorrhiza concentrate; Trichoderma spp (preferably Trichoderma harzianum, e.g. T22) 15 to 120, preferably 20 to 80, e.g. about 40 parts by weight; rock phosphate 2 to 20, preferably 3 to 10, e.g. about 5 parts by weight; carrier/dispersing agent composition 60 to 300, preferably 100 to 200, e.g. about 150 parts by weight.
  • the mixture is packaged, e.g. in bags, and sealed.
  • the mycorrhiza spore count of the product may be e.g. from 300 to 1500 spores per g, preferably about 700 spores/g.
  • the seed-coating powder is applied to seed by mixing together with an adhesive substance, e.g. sugar or molasses solution, or even beer, or compatible fungicides and pesticides in proportions depending on the crop and seed-planting density.
  • an adhesive substance e.g. sugar or molasses solution, or even beer, or compatible fungicides and pesticides in proportions depending on the crop and seed-planting density.
  • wheat may be treated in a proportion of 200 g seed- coating powder to 100 kg wheat seed/hectare.
  • seeds to which the powder may be applied are generally seeds not less than 2 mm in size and may include wheat, maize, barley, sorghum, mustard, cotton, luzerne, sun flowers, peas, beans, onions, to mention but a few examples, e.g. in a ratio of 200 g per 10 to 50 kg, e.g. per 25 kg of seed.
  • Seed coated as aforesaid falls within the scope of the present invention.
  • a growth medium is prepared consisting of 3 parts sand and 2 parts soil mixture containing zeolite powder, vermiculite, coconut fibre, peat, bentonite and apatite. This is placed in crates and Sudan grass or Korog is sown therein and grown for 3 to 4 months in nursery tunnels, the time being determined by frequent control checks on spore counts in the growth medium.
  • the grass, including the roots, is removed and the growth medium which contains spores and hyphae is then processed as follows: mixed in water and decanted through a series of sieves after allowing heavy sand particles to settle for a few seconds. The washing and decanting process is repeated until the water is clear (3 to 4 times). Roots and coarse debris are collected on a coarse 200 micron screen while spores are captured on 2 finer screens i.e. one screen from 38 to 45 microns and the other screen from 53 to 60 microns. Decant the spores on these 2 screens in water into a container. Add this water containing the spores to fine zeolite powder (10 to 150 micron size) / powder from any clay e.g.
  • apatite and bentonite work it through with the hands to obtain a nearly dry mixture (10 % moisture).
  • Sieve the mixture through a 120 micron sieve and mix with carrier/dispersing agent powder, i.e. any inorganic mineral (calcitic clay, silicious mineral, in particular perlite as also described in ZA patent 96/6318) or an organic soya powder.
  • carrier/dispersing agent powder i.e. any inorganic mineral (calcitic clay, silicious mineral, in particular perlite as also described in ZA patent 96/6318) or an organic soya powder.
  • carrier/dispersing agent powder i.e. any inorganic mineral (calcitic clay, silicious mineral, in particular perlite as also described in ZA patent 96/6318) or an organic soya powder.
  • carrier/dispersing agent powder i.e. any inorganic mineral (calcitic clay, silicious mineral, in particular perlite as also described in ZA patent 96/63
  • mycorrhiza concentrate 75 000 spores
  • Trichoderma harzianum T22 concentrate spore count 1 x 10 9 /g and 9 g Azotobacter concentrate (spore count 6 x 10 8 /g)
  • the formulation is suspended in water in a concentration depending on the crop and manner of application.
  • For potatoes 200 g of the composition are suspended in 10 L of water and then further diluted in 200 to 800 litres of water to be spread over 0,5 hectares.
  • the Trichoderma concentrate used in this and the following examples contains 1 x 10 9 active spores/g on a carrier mixture.
  • the Azotobacter concentrate contains 6 x 10 8 minimum colony-forming units/g on a carrier mixture.
  • the carrier mixture may be any suitable inorganic mineral (calcitic clay, silicious mineral, perlite as described in ZA patent 96/6318) or any suitable organic soya powder product.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

L'invention concerne un procédé de production d'inoculum endomycorhizien qui consiste à cultiver des spores endomycorhiziens sur des racines d'une plante hôte dans un milieu de croissance et qui implique des séquences d'étapes visant à séparer les spores et à produire un concentré de spores sur un support à numération de spores exceptionnellement élevée, à excellente dispersibilité et présentant des propriétés de culture dite 'biologique' satisfaisantes. Le concentré de spores, éventuellement combiné à des concentrés ou métabolites fongiques ou bactériens favorisant la croissance, peut être utilisé tel quel ou lorsqu'il est transformé en divers produits commerciaux de type formes granulaires légères à ajouter dans des sols, ou additifs pour de l'eau d'irrigation ou compositions pour l'imprégnation de semences afin d'augmenter la croissance de plantes et de produire un effet antagoniste contre les pathogènes et les nématodes dans les domaines de l'agriculture, de l'horticulture et de la foresterie.
EP08799583A 2007-07-09 2008-07-08 Inoculum de micro-organisme, procédé et compositions Withdrawn EP2176196A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA200705600 2007-07-09
PCT/ZA2008/000060 WO2009009805A1 (fr) 2007-07-09 2008-07-08 Inoculum de micro-organisme, procédé et compositions

Publications (2)

Publication Number Publication Date
EP2176196A1 true EP2176196A1 (fr) 2010-04-21
EP2176196A4 EP2176196A4 (fr) 2010-12-08

Family

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Family Applications (1)

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EP08799583A Withdrawn EP2176196A4 (fr) 2007-07-09 2008-07-08 Inoculum de micro-organisme, procédé et compositions

Country Status (3)

Country Link
EP (1) EP2176196A4 (fr)
WO (1) WO2009009805A1 (fr)
ZA (1) ZA200808624B (fr)

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PERSAD-CHINNERY S B ET AL: "ENHANCEMENT OF IN-VITRO SPORE GERMINATION OF GIGASPORA-ROSEA BY A CELLULASE PREPARATION" 1992, MYCOLOGICAL RESEARCH, VOL. 96, NR. 8, PAGE(S) 626-628 , XP002607574 ISSN: 0953-7562 * abstract * * page 627 * *
See also references of WO2009009805A1 *

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CN112341266A (zh) * 2020-10-26 2021-02-09 中阿沸石承德环保科技有限公司 一种沸石生物有机肥及其制造方法

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