EP2054717A2 - Procédé de détection de macromolécules électriquement conductrices - Google Patents

Procédé de détection de macromolécules électriquement conductrices

Info

Publication number
EP2054717A2
EP2054717A2 EP07801589A EP07801589A EP2054717A2 EP 2054717 A2 EP2054717 A2 EP 2054717A2 EP 07801589 A EP07801589 A EP 07801589A EP 07801589 A EP07801589 A EP 07801589A EP 2054717 A2 EP2054717 A2 EP 2054717A2
Authority
EP
European Patent Office
Prior art keywords
magnetic field
macromolecule
volume
macromolecules
ion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07801589A
Other languages
German (de)
English (en)
Inventor
Holger MÜHLBERGER
Werner Hoffmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Karlsruher Institut fuer Technologie KIT
Original Assignee
Forschungszentrum Karlsruhe GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Karlsruhe GmbH filed Critical Forschungszentrum Karlsruhe GmbH
Publication of EP2054717A2 publication Critical patent/EP2054717A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y25/00—Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus

Definitions

  • the apparatus suitable for carrying out the method according to the invention comprises

Abstract

L'invention concerne un procédé de détection, une à une ou en petit nombre, de macromolécules électriquement conductrices, de préférence d'acides nucléiques ou de protéines, qui se déplacent librement dans un fluide ioniquement conducteur. L'invention a pour objet de proposer un procédé de détection de macromolécules dans lequel les macromolécules ne doivent pas avoir été préalablement dotées d'un marqueur magnétique. Ce procédé doit également permettre la manipulation des macromolécules, surtout pour les transporter vers l'analyse, les séparer ou les concentrer. A cet effet, on place tout d'abord dans un volume (1) un fluide ioniquement conducteur dans lequel se trouve la macromolécule (4) exempte de marqueur magnétique. Ensuite, un champ électrique est appliqué à ce volume, ce qui crée un premier champ magnétique autour de la macromolécule. Enfin, au moyen d'un détecteur de champ magnétique (5), on détermine le champ magnétique ou sa modification dans le temps, ce qui permet de détecter la macromolécule.
EP07801589A 2006-08-22 2007-08-10 Procédé de détection de macromolécules électriquement conductrices Withdrawn EP2054717A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610039370 DE102006039370A1 (de) 2006-08-22 2006-08-22 Verfahren zur Analyse von Makromolekülen und Vorrichtung zur Durchführung des Verfahrens
PCT/EP2007/007079 WO2008022718A1 (fr) 2006-08-22 2007-08-10 Procédé de détection de macromolécules électriquement conductrices

Publications (1)

Publication Number Publication Date
EP2054717A2 true EP2054717A2 (fr) 2009-05-06

Family

ID=38698732

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07801589A Withdrawn EP2054717A2 (fr) 2006-08-22 2007-08-10 Procédé de détection de macromolécules électriquement conductrices

Country Status (3)

Country Link
EP (1) EP2054717A2 (fr)
DE (1) DE102006039370A1 (fr)
WO (1) WO2008022718A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014591A1 (fr) * 1999-08-21 2001-03-01 Fox John S Detection a grande sensibilite de biomolecules, au moyen de particules magnetiques
US6992482B2 (en) * 2000-11-08 2006-01-31 Jentek Sensors, Inc. Magnetic field sensor having a switchable drive current spatial distribution
US6736978B1 (en) * 2000-12-13 2004-05-18 Iowa State University Research Foundation, Inc. Method and apparatus for magnetoresistive monitoring of analytes in flow streams
US7560927B2 (en) * 2003-08-28 2009-07-14 Massachusetts Institute Of Technology Slitted and stubbed microstrips for high sensitivity, near-field electromagnetic detection of small samples and fields

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2008022718A1 *

Also Published As

Publication number Publication date
WO2008022718A1 (fr) 2008-02-28
DE102006039370A1 (de) 2008-02-28
WO2008022718A8 (fr) 2008-09-25

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