EP2049881A2 - System and automated device for analysing a cell suspension - Google Patents

System and automated device for analysing a cell suspension

Info

Publication number
EP2049881A2
EP2049881A2 EP07823382A EP07823382A EP2049881A2 EP 2049881 A2 EP2049881 A2 EP 2049881A2 EP 07823382 A EP07823382 A EP 07823382A EP 07823382 A EP07823382 A EP 07823382A EP 2049881 A2 EP2049881 A2 EP 2049881A2
Authority
EP
European Patent Office
Prior art keywords
well
cell suspension
analysis
cells
suspension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07823382A
Other languages
German (de)
French (fr)
Inventor
Eric Peltier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novacyt SA
Original Assignee
Novacyt SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novacyt SA filed Critical Novacyt SA
Publication of EP2049881A2 publication Critical patent/EP2049881A2/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions

Definitions

  • the present invention relates to a system for analyzing a cell suspension and an analysis automaton comprising such a system.
  • These analyzes may for example include colorations whose purpose is to give a contrast of color and density at the nuclei and cells.
  • Solutions have also been proposed to improve this situation.
  • One of these solutions is, for example, the deposition of thin layers of the cells. These deposits may for example be made by decantation of a cell suspension, such as a cytological suspension, to allow the deposition of these cells on an assay plate.
  • This assay plate is then examined for pathological cells.
  • these methods also have a number of disadvantages, since the pathological cells can be relatively difficult to detect among the other deposits obtained.
  • the subject of the invention is a system for analyzing a cell suspension, characterized in that it comprises at least one sludge settling well and means for analyzing the cell deposits at the bottom of the slurry. well in time and during settling, to allow analysis of the cells by strata according to their settling speed.
  • the analysis means comprise means of illumination of the bottom of the well and means of shooting deposits;
  • the well has an open end closable by opaque means
  • the opaque means are formed by a self-healing membrane
  • the cell suspension is a cytological suspension.
  • the subject of the invention is also an automaton for analyzing a cell suspension comprising an analysis system according to the invention, means for sampling a cell suspension sample in a receptacle, and means for dispensing this suspension. sample in a well of settling of the analysis system.
  • FIG. 1 represents a schematic sectional view of an analysis system according to the invention.
  • FIG. 2 represents a schematic sectional view of an analysis automaton comprising such a system.
  • FIG. 1 shows a system for analyzing a cell suspension, such as, for example, a fixed cytological suspension.
  • Such a cytological suspension may for example come from a screening operation by cervical smear, for example using a sampling brush.
  • the brush is then dipped into a vial containing a fixative to collect the cells and thereby form the cell suspension.
  • This cell suspension is then placed in a settling well, as designated by the general reference 1 in this figure. During the decantation, the cells are then deposited at the bottom of this well.
  • the settling well is associated with means of analysis of these cell deposits at the bottom of the well in time and during the decantation process, to allow analysis of the cells by strata according to their settling speed.
  • the pathological cells are heavier than the other cells and that these cells are therefore deposited first during the decantation process.
  • These may for example be constituted by conventional means of illumination and lighting means, of the type for example camera, camera or other, or by more elaborate means implementing for example means for generating images.
  • broad or specific spectrum optical beams making it possible to reveal certain characteristics of the cells in order to detect the cells of interest or to better characterize them. It is therefore conceivable that an analysis of cells over time during the decantation process makes it possible to analyze each deposit layer and to facilitate the detection of pathological cells.
  • the well and more particularly its open end, may be associated with opaque means such as, for example, an opaque and for example self-healing closure membrane, making it possible to form in the settling well a dark chamber facilitating the deposit analysis.
  • opaque means such as, for example, an opaque and for example self-healing closure membrane, making it possible to form in the settling well a dark chamber facilitating the deposit analysis.
  • Such an analysis system can for example be integrated in an analysis automaton such as that illustrated in FIG. 2 and designated by the general reference 4.
  • sampling means 6 makes it possible, for example, for the sampling means 6 to take a cell suspension sample from any container 7, placed by an operator in the automaton 4, in order to transfer this sample to the settling well in an automated manner.

Abstract

This system for analysing a cell suspension is characterized in that it comprises at least one well for decanting (1) the suspension and means (2, 3) for analysing the cell deposits at the bottom of the well over time during the decanting, so as to allow an analysis of the cells by strata (T1, T2, T3) as a function of their rate of decantation.

Description

Système et automate d'analyse d'une suspension cellulaire System and automaton for analyzing a cell suspension
La présente invention concerne un système d'analyse d'une suspension cellulaire et un automate d'analyse comportant un tel système.The present invention relates to a system for analyzing a cell suspension and an analysis automaton comprising such a system.
L'analyse d'une suspension cellulaire et par exemple d'un étalement cytologique fixé est une opération extrêmement délicate. Le but d'une telle analyse est en effet de dépister des cellules pathologiques.The analysis of a cell suspension and for example a fixed cytological spread is an extremely delicate operation. The purpose of such an analysis is indeed to detect pathological cells.
Différentes techniques d'analyse ont déjà été proposées dans l'état de la technique.Different analysis techniques have already been proposed in the state of the art.
Ces analyses peuvent par exemple comporter des colorations dont le but est de donner un contraste de couleur et de densité au niveau des noyaux et des cellules.These analyzes may for example include colorations whose purpose is to give a contrast of color and density at the nuclei and cells.
Cependant, cette coloration n'est en aucun cas spécifique et ne marque pas les cellules tumorales de façon particulière. La reconnaissance des cellules dysplasiques ou tumorales se fait alors sur les aspects des noyaux et en particulier leur taille, leur forme, leur densité, etc. et sur le rapport nucléocytoplasmique. Une difficulté supplémentaire est liée au faible nombre de cas pathologiques (moins de 5%) et au faible nombre de cellules pathologiques par cas (moins de 0,2%) qui sont parfois masquées par des débris cellulaires ou de l'inflammation. De ce fait, le travail d'un cytologiste formé à sélectionner les cas pathologiques est extrêmement astreignant, peu valorisant et source de tension.However, this coloration is in no way specific and does not mark the tumor cells in a particular way. The recognition of dysplastic or tumor cells is then done on the aspects of the nuclei and in particular their size, shape, density, etc. and on the nucleocytoplasmic ratio. An additional difficulty is linked to the small number of pathological cases (less than 5%) and the small number of pathological cells per case (less than 0.2%) which are sometimes masked by cell debris or inflammation. As a result, the work of a trained cytologist to select pathological cases is extremely demanding, not very rewarding and a source of tension.
Des solutions ont également été proposées pour améliorer cette situation. L'une de ces solutions est par exemple le dépôt en couches minces des cellules. Ces dépôts peuvent par exemple être réalisés par décantation d'une suspension cellulaire, telle qu'une suspension cytologique, afin de permettre le dépôt de ces cellules sur une plaque d'analyse.Solutions have also been proposed to improve this situation. One of these solutions is, for example, the deposition of thin layers of the cells. These deposits may for example be made by decantation of a cell suspension, such as a cytological suspension, to allow the deposition of these cells on an assay plate.
Cette plaque d'analyse est ensuite examinée pour détecter les cellules pathologiques. Cependant, ces méthodes présentent également un certain nombre d'inconvénients, car les cellules pathologiques peuvent être relativement difficiles à détecter parmi les autres dépôts obtenus.This assay plate is then examined for pathological cells. However, these methods also have a number of disadvantages, since the pathological cells can be relatively difficult to detect among the other deposits obtained.
Le but de l'invention est donc de résoudre ces problèmes. A cet effet, l'invention a pour objet un système d'analyse d'une suspension cellulaire, caractérisé en ce qu'il comporte au moins un puits de décantation de la suspension et des moyens d'analyse des dépôts de cellules au fond du puits dans le temps et au cours de la décantation, pour permettre une analyse des cellules par strates en fonction de leur vitesse de décantation.The object of the invention is therefore to solve these problems. For this purpose, the subject of the invention is a system for analyzing a cell suspension, characterized in that it comprises at least one sludge settling well and means for analyzing the cell deposits at the bottom of the slurry. well in time and during settling, to allow analysis of the cells by strata according to their settling speed.
Selon d'autres caractéristiques de l'invention :According to other features of the invention:
- les moyens d'analyse comprennent des moyens d'éclairage du fond du puits et des moyens de prise de vue des dépôts ;the analysis means comprise means of illumination of the bottom of the well and means of shooting deposits;
- le puits présente une extrémité ouverte obturable par des moyens opaques ;- The well has an open end closable by opaque means;
- les moyens opaques sont formés par une membrane autocicatrisante ; etthe opaque means are formed by a self-healing membrane; and
- la suspension cellulaire est une suspension cytologique. L'invention a également pour objet un automate d'analyse d'une suspension cellulaire comportant un système d'analyse selon l'invention, des moyens de prélèvement d'un échantillon de suspension cellulaire dans un récipient, et des moyens de versement de cet échantillon dans un puits de décantation du système d'analyse.the cell suspension is a cytological suspension. The subject of the invention is also an automaton for analyzing a cell suspension comprising an analysis system according to the invention, means for sampling a cell suspension sample in a receptacle, and means for dispensing this suspension. sample in a well of settling of the analysis system.
L'invention sera mieux comprise à la lecture de la description qui va suivre, donnée uniquement à titre d'exemple et faite en se référant aux dessins annexés, sur lesquels :The invention will be better understood on reading the description which follows, given solely by way of example and with reference to the appended drawings, in which:
- la figure 1 représente une vue en coupe schématique d'un système d'analyse selon l'invention ; etFIG. 1 represents a schematic sectional view of an analysis system according to the invention; and
- la figure 2 représente une vue en coupe schématique d'un automate d'analyse comportant un tel système.FIG. 2 represents a schematic sectional view of an analysis automaton comprising such a system.
On a en effet illustré sur la figure 1 , un système d'analyse d'une suspension cellulaire, telle que par exemple une suspension cytologique fixée.Indeed, FIG. 1 shows a system for analyzing a cell suspension, such as, for example, a fixed cytological suspension.
Une telle suspension cytologique peut par exemple provenir d'une opération de dépistage par frottis du col de l'utérus, par exemple à l'aide d'une brosse de prélèvement.Such a cytological suspension may for example come from a screening operation by cervical smear, for example using a sampling brush.
La brosse est ensuite plongée dans un flacon contenant un fixateur pour recueillir les cellules et former ainsi la suspension cellulaire.The brush is then dipped into a vial containing a fixative to collect the cells and thereby form the cell suspension.
Cette suspension cellulaire est ensuite placée dans un puits de décantation, tel que désigné par la référence générale 1 sur cette figure. Au cours de la décantation, les cellules viennent alors se déposer au fond de ce puits.This cell suspension is then placed in a settling well, as designated by the general reference 1 in this figure. During the decantation, the cells are then deposited at the bottom of this well.
Dans le système selon l'invention, le puits de décantation est associé à des moyens d'analyse de ces dépôts de cellules au fond du puits dans le temps et au cours du processus de décantation, pour permettre une analyse des cellules par strates en fonction de leur vitesse de décantation.In the system according to the invention, the settling well is associated with means of analysis of these cell deposits at the bottom of the well in time and during the decantation process, to allow analysis of the cells by strata according to their settling speed.
En effet, il a été constaté que les cellules pathologiques sont plus lourdes que les autres cellules et que celles-ci se déposent donc en premier lors du processus de décantation. En analysant le processus de dépôt de ces cellules, au cours du processus de décantation et en particulier le dépôt des cellules les plus lourdes, il est alors possible de faciliter la détection des cas pathologiques.Indeed, it has been found that the pathological cells are heavier than the other cells and that these cells are therefore deposited first during the decantation process. By analyzing the deposition process of these cells, during the decantation process and in particular the deposition of the heavier cells, it is then possible to facilitate the detection of pathological cases.
Ceci peut par exemple être réalisé par l'intermédiaire de moyens de prise de vue des dépôts au cours du processus de décantation, ces moyens de prise de vue étant désignés par la référence générale 2 sur cette figure et étant associés à des moyens d'éclairage du puits et par exemple du fond de ce puits, ces moyens d'éclairage étant désignés par la référence générale 3.This can for example be achieved through means of shooting deposits during the decantation process, these means of shooting being designated by the general reference 2 in this figure and being associated with lighting means well and for example the bottom of this well, these lighting means being designated by the general reference 3.
On conçoit alors que le suivi du processus de décantation permet par exemple d'avoir des analyses des cellules déposées en fonction de leur poids et au cours du temps.It is thus conceivable that the monitoring of the settling process makes it possible, for example, to have analyzes of the cells deposited according to their weight and over time.
Ceci permet par exemple d'obtenir des analyses par strates de dépôt, telles que par exemple les strates désignées par les références T1 , T2 et T3 sur cette figure 1.This makes it possible, for example, to obtain analyzes by deposit layers, such as, for example, the strata designated by the references T1, T2 and T3 in this FIG.
Une image de chaque strate de dépôt permet alors de faciliter la détection des cellules pathologiques.An image of each deposit layer then makes it easier to detect the pathological cells.
Bien entendu, différents types de moyens d'analyse peuvent être envisagés.Of course, different types of analysis means can be envisaged.
Ceux-ci peuvent par exemple être constitués par des moyens de prise de vue et des moyens d'éclairage classiques, de type par exemple caméra, appareil photo ou autre, ou par des moyens plus élaborés mettant en œuvre par exemple des moyens de génération de faisceaux optiques à spectre large ou spécifique, permettant de faire apparaître certaines particularités des cellules afin de détecter les cellules d'intérêt ou de mieux les caractériser. On conçoit alors qu'une analyse des cellules dans le temps, au cours du processus de décantation, permet d'analyser chaque strate de dépôt et de faciliter la détection des cellules pathologiques.These may for example be constituted by conventional means of illumination and lighting means, of the type for example camera, camera or other, or by more elaborate means implementing for example means for generating images. broad or specific spectrum optical beams, making it possible to reveal certain characteristics of the cells in order to detect the cells of interest or to better characterize them. It is therefore conceivable that an analysis of cells over time during the decantation process makes it possible to analyze each deposit layer and to facilitate the detection of pathological cells.
On notera également que le puits, et plus particulièrement son extrémité ouverte, peut être associée à des moyens opaques comme par exemple une membrane d'obturation opaque et par exemple autocicatrisante, permettant de former dans le puits de décantation, une chambre noire facilitant l'analyse des dépôts.It will also be noted that the well, and more particularly its open end, may be associated with opaque means such as, for example, an opaque and for example self-healing closure membrane, making it possible to form in the settling well a dark chamber facilitating the deposit analysis.
Bien entendu, d'autres moyens peuvent être envisagés pour obturer ce puits et former une chambre noire.Of course, other means can be envisaged to seal this well and form a dark room.
Un tel système d'analyse peut par exemple être intégré dans un automate d'analyse tel que celui illustré sur la figure 2 et désigné par la référence générale 4.Such an analysis system can for example be integrated in an analysis automaton such as that illustrated in FIG. 2 and designated by the general reference 4.
On reconnaît en effet sur cette figure 2, le système de dépôt décrit en regard de la figure 1 , avec le puits de décantation 1 , les moyens de prise de vue 2, les moyens d'éclairage 3.2, the deposition system described with reference to FIG. 1, with the settling well 1, the means of shooting 2, the lighting means 3.
Ces moyens de prise de vue et d'éclairage sont alors associés par exemple à un contrôleur de pilotage du fonctionnement de l'automate qui commande également le fonctionnement d'un bras de manipulation désigné par la référence générale 5 et de moyens de transfert de suspension désignés par la référence générale 6.These means of shooting and lighting are then associated for example with a controller for controlling the operation of the automaton which also controls the operation of a manipulation arm designated by the general reference 5 and suspension transfer means designated by general reference 6.
Ceci permet alors par exemple aux moyens de prélèvement 6 de prélever un échantillon de suspension cellulaire dans un récipient quelconque 7, placé par un opérateur dans l'automate 4, afin de transférer cet échantillon dans le puits de décantation et ce de façon automatisée.This then makes it possible, for example, for the sampling means 6 to take a cell suspension sample from any container 7, placed by an operator in the automaton 4, in order to transfer this sample to the settling well in an automated manner.
Les résultats de l'analyse sont alors délivrés par le contrôleur de pilotage qui est désigné par la référence générale 8.The results of the analysis are then delivered by the steering controller which is designated by the general reference 8.
On conçoit alors qu'une telle structure permet d'automatiser complètement l'analyse. Bien entendu, d'autres modes de réalisation encore peuvent être envisagés. It can be seen that such a structure makes it possible to completely automate the analysis. Of course, other embodiments may be envisaged.

Claims

REVENDICATIONS
1. Système d'analyse d'une suspension cellulaire, caractérisé en ce qu'il comporte au moins un puits de décantation (1) de la suspension et des moyens (2,3) d'analyse des dépôts de cellules au fond du puits dans le temps au cours de la décantation, pour permettre une analyse des cellules par strates (T1 , T2, T3) en fonction de leur vitesse de décantation.1. System for analyzing a cell suspension, characterized in that it comprises at least one settling well (1) of the suspension and means (2,3) for analyzing cell deposits at the bottom of the well. in time during decantation, to allow analysis of the cells by strata (T1, T2, T3) as a function of their settling speed.
2. Système d'analyse selon la revendication 1 , caractérisé en ce que les moyens d'analyse comprennent des moyens d'éclairage (3) du puits et des moyens (2) de prise de vue des dépôts.2. Analysis system according to claim 1, characterized in that the analysis means comprise lighting means (3) of the well and means (2) for shooting deposits.
3. Système d'analyse selon la revendication 1 ou 2, caractérisé en ce que le puits (1) présente une extrémité ouverte obturable par des moyens opaques.3. Analysis system according to claim 1 or 2, characterized in that the well (1) has an open end closable by opaque means.
4. Système d'analyse selon la revendication 3, caractérisé en ce que les moyens opaques sont formés par une membrane autocicatrisante.4. Analysis system according to claim 3, characterized in that the opaque means are formed by a self-healing membrane.
5. Système d'analyse selon l'une quelconque des revendications précédentes, caractérisé en ce que la suspension cellulaire est une suspension cytologique.5. Analysis system according to any one of the preceding claims, characterized in that the cell suspension is a cytological suspension.
6. Automate d'analyse d'une suspension cellulaire, caractérisé en ce qu'il comporte un système d'analyse selon l'une quelconque des revendications précédentes.6. Automate for analyzing a cell suspension, characterized in that it comprises an analysis system according to any one of the preceding claims.
7. Automate selon la revendication 6, caractérisé en ce qu'il comporte en outre des moyens (6) de prélèvement d'un échantillon de suspension cellulaire dans un récipient (7) et des moyens (5,6) de versement de cet échantillon dans un puits de décantation (1) du système d'analyse. 7. An automaton according to claim 6, characterized in that it further comprises means (6) for taking a sample of cell suspension in a container (7) and means (5,6) for pouring this sample. in a settling well (1) of the analysis system.
EP07823382A 2006-08-11 2007-08-01 System and automated device for analysing a cell suspension Withdrawn EP2049881A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0607311A FR2904871B1 (en) 2006-08-11 2006-08-11 SYSTEM AND AUTOMATUS FOR ANALYZING A CELLULAR SUSPENSION
PCT/FR2007/001329 WO2008020122A2 (en) 2006-08-11 2007-08-01 System and automated device for analysing a cell suspension

Publications (1)

Publication Number Publication Date
EP2049881A2 true EP2049881A2 (en) 2009-04-22

Family

ID=37680691

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07823382A Withdrawn EP2049881A2 (en) 2006-08-11 2007-08-01 System and automated device for analysing a cell suspension

Country Status (5)

Country Link
US (2) US20100167387A1 (en)
EP (1) EP2049881A2 (en)
CN (1) CN101501471A (en)
FR (1) FR2904871B1 (en)
WO (1) WO2008020122A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3009622B1 (en) * 2013-08-09 2015-08-07 Novacyt METHOD AND DEVICE FOR WASHING A PIPETAGE-DISTRIBUTION DEVICE
CN109085098A (en) * 2017-06-14 2018-12-25 北京精微视觉科技有限公司 A kind of detection method and detection device of cell settlement progress

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2725782A (en) * 1952-08-19 1955-12-06 Worley Carl Milton Apparatus for recording rate of sedimentation in liquids
US3288019A (en) * 1962-05-08 1966-11-29 Charles M Blumenfeld Photographic type sedimentation recorder
US3474458A (en) * 1968-04-03 1969-10-21 Adrian W Standaart Automatic blood sedimentation rate recorder
US4393466A (en) * 1980-09-12 1983-07-12 International Remote Imaging Systems Method of analyzing particles in a dilute fluid sample
US4871248A (en) * 1987-08-11 1989-10-03 Monsanto Company Method of particle size determination
US5699794A (en) * 1995-12-19 1997-12-23 Neopath, Inc. Apparatus for automated urine sediment sample handling
US20030129665A1 (en) * 2001-08-30 2003-07-10 Selvan Gowri Pyapali Methods for qualitative and quantitative analysis of cells and related optical bio-disc systems
US7391515B2 (en) * 2004-10-05 2008-06-24 Gerald Walter Budd Automated visual inspection system for the detection of microbial growth in solutions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2008020122A2 *

Also Published As

Publication number Publication date
WO2008020122A2 (en) 2008-02-21
CN101501471A (en) 2009-08-05
US20100167387A1 (en) 2010-07-01
FR2904871A1 (en) 2008-02-15
WO2008020122A3 (en) 2008-04-17
US20120301915A1 (en) 2012-11-29
FR2904871B1 (en) 2009-03-06

Similar Documents

Publication Publication Date Title
Blom et al. Stimulated emission depletion microscopy
EP3304214B1 (en) Method for observing a sample
Sharonov et al. Confocal three‐dimensional scanning laser Raman–SERS–fluorescence microprobe. Spectral imaging and high‐resolution applications
EP3025144B1 (en) Cell-sorting method and associated device
FR2826882A1 (en) Manipulation of magnetic particles, and associated biological matter, uses relative movements of the container and the magnet, to align the particles into filaments in the solution on the magnet north-south axis
EP1963859A1 (en) Method of discriminating at least two cell populations, and application
EP3559631A1 (en) Method for counting particles in a sample by means of lensless imaging
EP2049881A2 (en) System and automated device for analysing a cell suspension
EP2212733B1 (en) Method and device for the reconstruction of the shape of an object from a sequence of sectional images of said object
EP3058336B1 (en) Unit for measuring the settling velocity of particles in suspension in a fluid and device comprising at least said unit and an automatic sampler
EP3230713B1 (en) Method for obtaining an image of a sample, and associated lens-free imaging system
FR2931966A1 (en) METHOD FOR CELLULAR ANALYSIS OF A SAMPLING USING A VIRTUAL ANALYSIS PLATE
EP3938831A1 (en) Device and method for observing microparticles and nanoparticles
EP2347296A1 (en) Procecure for preparing a processed virtual analysis image
WO2019186073A1 (en) Method for detecting cells having at least one anomaly in a cytological sample
FR3082943A1 (en) METHOD FOR COUNTING SMALL PARTICLES IN A SAMPLE
EP0964363B1 (en) Process for separating image sequence static and dynamic components
EP1045240A1 (en) Method for preparing a cell suspension
Zhou et al. Narrowband photoblinking InP/ZnSe/ZnS quantum dots for super-resolution multifocal structured illumination microscopy enhanced by optical fluctuation
FR3075372A1 (en) DEVICE AND METHOD FOR OBSERVING A SAMPLE WITH A CHROMATIC OPTICAL SYSTEM
EP3423882B1 (en) Quantitative non-linear optical microscope
Stoneman et al. Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants
EP3629004A1 (en) Method and device for observing a sample under ambient light
EP4276459A1 (en) Method and system for detecting presence of embryo organ in egg
CA2530382A1 (en) Method for dosing a biological or chemical sample

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20090206

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA HR MK RS

17Q First examination report despatched

Effective date: 20090722

DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20140301