EP1967200A1 - Formulations of alpha-amylase inhibitors of P. vulgaris with alpha-glucosidade inhibitores of S. oblonga or S. reticulata useful in the treatment of diabetes and obesity - Google Patents
Formulations of alpha-amylase inhibitors of P. vulgaris with alpha-glucosidade inhibitores of S. oblonga or S. reticulata useful in the treatment of diabetes and obesity Download PDFInfo
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- EP1967200A1 EP1967200A1 EP07425134A EP07425134A EP1967200A1 EP 1967200 A1 EP1967200 A1 EP 1967200A1 EP 07425134 A EP07425134 A EP 07425134A EP 07425134 A EP07425134 A EP 07425134A EP 1967200 A1 EP1967200 A1 EP 1967200A1
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- EP
- European Patent Office
- Prior art keywords
- alpha
- formulations
- oblonga
- salacia
- phaseolus vulgaris
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- 239000000203 mixture Substances 0.000 title claims description 25
- 238000009472 formulation Methods 0.000 title claims description 12
- 208000008589 Obesity Diseases 0.000 title description 4
- 235000020824 obesity Nutrition 0.000 title description 4
- 238000011282 treatment Methods 0.000 title description 2
- 244000110797 Polygonum persicaria Species 0.000 title 1
- 240000004794 Sanchezia nobilis Species 0.000 title 1
- 244000058639 Stephania reticulata Species 0.000 title 1
- 206010012601 diabetes mellitus Diseases 0.000 title 1
- 239000000284 extract Substances 0.000 claims abstract description 45
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 21
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- 235000019813 microcrystalline cellulose Nutrition 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- 230000009467 reduction Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
- 229930195725 Mannitol Natural products 0.000 description 4
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 4
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 4
- 239000001768 carboxy methyl cellulose Substances 0.000 description 4
- 239000007903 gelatin capsule Substances 0.000 description 4
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 4
- 239000000594 mannitol Substances 0.000 description 4
- 235000010355 mannitol Nutrition 0.000 description 4
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- 229940069328 povidone Drugs 0.000 description 4
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- 239000000377 silicon dioxide Substances 0.000 description 4
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- 229950002273 simeticone Drugs 0.000 description 4
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 4
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 4
- 108010028144 alpha-Glucosidases Proteins 0.000 description 3
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- 239000002028 Biomass Substances 0.000 description 2
- 102100025841 Cholecystokinin Human genes 0.000 description 2
- 101800001982 Cholecystokinin Proteins 0.000 description 2
- 229920002785 Croscarmellose sodium Polymers 0.000 description 2
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 2
- 241000219833 Phaseolus Species 0.000 description 2
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical compound CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
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- 229940024171 alpha-amylase Drugs 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
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- 229940107137 cholecystokinin Drugs 0.000 description 2
- 239000001767 crosslinked sodium carboxy methyl cellulose Substances 0.000 description 2
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- 238000000605 extraction Methods 0.000 description 2
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- 231100000331 toxic Toxicity 0.000 description 2
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- 239000001069 triethyl citrate Substances 0.000 description 2
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 description 2
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- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
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- 238000011026 diafiltration Methods 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
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- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000030135 gastric motility Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 239000012729 immediate-release (IR) formulation Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/37—Celastraceae (Staff-tree or Bittersweet family), e.g. tripterygium or spindletree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to formulations consisting of (i) Phaseolus vulgaris extracts containing pharmacologically active quantities of ⁇ -amylase inhibitors and (ii) Salacia oblonga or Salacia reticulata extracts containing pharmacologically active quantities of ⁇ -glucosidase inhibitors.
- Obesity is currently one of the major health problems, especially in the industrialised countries, with serious consequences in cardiocirculatory and skeletal terms.
- Carbohydrates are an important source of calories, and contribute to the synthesis of fats in individuals predisposed to obesity or type II diabetes. As hyperglycaemia leads to an increase in energy deposits, the availability of substances that reduce bioavailable glucose is very important. As starches are the main source of glucose, specific ⁇ -glucosidase and ⁇ -amylase inhibitors, obtained from plant materials or by synthesis, have been studied. It has long been known that some seeds and pulses contain substances which can have adverse effects on the diet if eaten before they are completely cooked. Many pulses contain protease inhibitors, amylase inhibitors and substances that discourage predators from continuing to use them by reducing the appetite. These substances, called phytohaemagglutinins, can cause hyperplasia of the pancreas at high doses, but can be useful in appetite control at lower doses.
- cholecystokinin a trophic hormone that stimulates secretion by the pancreas, consequently causing its enlargement. Cholecystokinin also has favourable effects, because it reduces the appetite by reducing gastric motility.
- glucose originates from the breakdown of starch, which begins in the mouth due to the effect of ptyalin; this enzyme detaches saccharide chains, which in turn are converted to glucose by saccharases and ⁇ -glucosidases.
- ⁇ -amylase secreted by the pancreas in the duodenum, demolishes the starch in glucose chains in the intestine, where it is converted to glucose due to the effect of ⁇ -glucosidase.
- the ⁇ -amylase inhibitor in itself considerably reduces the quantity of glucose originating from the starches present in the diet, and reduces the appetite after repeated administration.
- Said extract can be obtained by a process which comprises:
- AUC of glucose plasma levels 0 0 6850 ⁇ 600 100 0 5500 ⁇ 700 * (-20%) 0 200 5600 ⁇ 650 * (-18%) 50 100 4500 ⁇ 900 ** (-35%) Number of animal/group: 8 * p ⁇ 0.05 ** p ⁇ 0.01 vs controls
- the present invention allows the preparation of products useful in the treatment of obesity, excess weight and type II diabetes, and in diets designed to maintain a constant body weight.
- the two extracts are combined in a ratio of between 1:1 and 1:4; the doses range from 50 to 200 mg for the Phaseolus vulgaris extract and from 200 to 600 mg for the Salacia extract, depending on the active constituent content measured by HPLC.
- the formulations according to the invention can take the form of soft or hard gelatin capsules, cellulose capsules, tablets or liquid forms, and the extracts can be separately formulated in totally or partly gastroresistant forms.
- the products should be taken at main meals or whenever foods rich in carbohydrates are eaten.
- the formulation can also be associated with substances that reduce gas formation in the colon or partly absorb the excess.
- Example 1 Formulation of Phaseolus vulgaris and Salacia oblonga extracts into hard gelatin capsules
- Example 2 Formulation of Phaseolus vulgaris and Salacia oblonga extracts into tablets
- Phaseolus vulgaris dried extract 100 mg Salacia oblonga dried extract 150 mg
- Mannitol 100 mg
- Cross-linked sodium carboxymethylcellulose 30 mg
- Silicon dioxide 8 mg
- Example 3 Formulation of Phaseolus vulgaris and Salacia oblonga extracts into modified-release granules
- Phaseolus vulgaris extract 50 mg Salacia oblonga extract 100 mg Microcrystalline cellulose 100 mg Povidone 10 mg Sodium carboxymethylcellulose 8 mg Methacrylic acid copolymer 50 mg Triethyl citrate 3.2 mg Talc 8 mg Simeticone 0.3 mg
- Example 4 Formulation of Phaseolus vulgaris and Salacia oblonga extracts into immediate-release granules
- Example 5 Mixture of granulates of Phaseolus vulgaris and Salacia oblonga extracts with different release profiles
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Obesity (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Child & Adolescent Psychology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
- The present invention relates to formulations consisting of (i) Phaseolus vulgaris extracts containing pharmacologically active quantities of α-amylase inhibitors and (ii) Salacia oblonga or Salacia reticulata extracts containing pharmacologically active quantities of α-glucosidase inhibitors.
- Obesity is currently one of the major health problems, especially in the industrialised countries, with serious consequences in cardiocirculatory and skeletal terms.
- Carbohydrates are an important source of calories, and contribute to the synthesis of fats in individuals predisposed to obesity or type II diabetes. As hyperglycaemia leads to an increase in energy deposits, the availability of substances that reduce bioavailable glucose is very important. As starches are the main source of glucose, specific α-glucosidase and α-amylase inhibitors, obtained from plant materials or by synthesis, have been studied. It has long been known that some seeds and pulses contain substances which can have adverse effects on the diet if eaten before they are completely cooked. Many pulses contain protease inhibitors, amylase inhibitors and substances that discourage predators from continuing to use them by reducing the appetite. These substances, called phytohaemagglutinins, can cause hyperplasia of the pancreas at high doses, but can be useful in appetite control at lower doses.
- At high doses, these lectins survive the intestinal transit and bond to the enterocytes where they cause the secretion of cholecystokinin, a trophic hormone that stimulates secretion by the pancreas, consequently causing its enlargement. Cholecystokinin also has favourable effects, because it reduces the appetite by reducing gastric motility.
- As excess blood glucose leads to an increase in energy deposits, substances that reduce the availability of free glucose after an intake of starchy carbohydrates in the diet are very important. Most glucose originates from the breakdown of starch, which begins in the mouth due to the effect of ptyalin; this enzyme detaches saccharide chains, which in turn are converted to glucose by saccharases and α-glucosidases. α-amylase, secreted by the pancreas in the duodenum, demolishes the starch in glucose chains in the intestine, where it is converted to glucose due to the effect of α-glucosidase.
- It is therefore obvious that in order to achieve a substantial reduction in the release of glucose, it is important to have both α-amylase inhibitors and α-glucosidase inhibitors.
- The α-amylase inhibitor in itself considerably reduces the quantity of glucose originating from the starches present in the diet, and reduces the appetite after repeated administration.
- It has now surprisingly been found that by combining Phaseolus vulgaris extracts with Salacia oblonga extracts in the ratio of between 1:1 and 1:4, a marked reduction in blood glucose is obtained in humans. It is also known that a reduced food intake leads in any event to a reduction in the accumulation of fatty deposits.
- It has surprisingly been found that by preparing products enriched with α-amylase inhibitors having a lectin content that ensures excellent tolerability, a reduction in body weight proportional to the dose administered can be obtained. The data in rats suggested that the effect on body weight reduction was not simply associated with a reduction in the plasma glucose level, but also with a definite reduction in food consumption. Various pharmacological experiments demonstrated that this reduced food intake, despite unrestricted access to food, was associated not with a simply toxic effect, but with a modification in the desire to eat. The Phaseolus vulgaris extract described in
PCT/EP2006/012012 - a) extraction from Phaseolus sp. with aqueous buffers having a pH varying between 3 and 6.5 and subsequent separation of the extract from the biomass, which can possibly be further extracted with the buffer until exhaustion in alpha-amylase and phytohaemagglutinin inhibitors;
- b) filtration or centrifugation of the combined extracts and concentration to a volume corresponding to approx. 10% of the weight of the biomass of the initial extract after centrifugation;
- c) differential precipitation of the concentrated aqueous extract with diluted ethanol, to a final concentration of between 60 and 70% v/v;
- d) separation of the precipitate and reprecipitation from demineralised water with 60% ethanol, or diafiltration on a membrane with a 10,000 Da cut-off and drying of the precipitation residue.
- The combination of an extract with α-amylase inhibitors and an extract containing α-glucosidase inhibitors, such as extracts of Salacia oblonga or Salacia reticulata root or similar commercially obtainable species, has surprisingly demonstrated an unforeseen synergic effect, with a reduction in plasma glucose levels not obtainable with the individual ingredients.
- The results for the hypoglycaemic activity of the combination and the individual extracts are set out in the Table below according to the method described by Tormo MA et al., Br. J. Nutr. 96, 539, 2006.
Table Effect of Phaseolus vulgaris extract, Salacia oblonga extract and their combination on glycemia in Wistar rats given a restricted amount of food with a 1 hour/day limited access Phaseolus vulgaris extract mg/kg p.o. Salacia oblonga extract mg/kg p.o. AUC of glucose plasma levels (mg/dL) 0 0 6850 ± 600 100 0 5500 ± 700 * (-20%) 0 200 5600 ± 650 * (-18%) 50 100 4500 ± 900 ** (-35%) Number of animal/group: 8
* p<0.05 ** p<0.01 vs controls - The administration of the individual compounds and the corresponding combination did not have any toxic effect at doses of up to 2000 mg/kg for three months.
- The present invention allows the preparation of products useful in the treatment of obesity, excess weight and type II diabetes, and in diets designed to maintain a constant body weight.
- In order to be effective on blood glucose and to be effective on weight control in long-term treatments, the two extracts are combined in a ratio of between 1:1 and 1:4; the doses range from 50 to 200 mg for the Phaseolus vulgaris extract and from 200 to 600 mg for the Salacia extract, depending on the active constituent content measured by HPLC. The formulations according to the invention can take the form of soft or hard gelatin capsules, cellulose capsules, tablets or liquid forms, and the extracts can be separately formulated in totally or partly gastroresistant forms. The products should be taken at main meals or whenever foods rich in carbohydrates are eaten. The formulation can also be associated with substances that reduce gas formation in the colon or partly absorb the excess.
- The following examples illustrate the invention in detail.
-
Unit composition: Phaseolus vulgaris dried extract 50 mg Salacia oblonga dried extract 200 mg Microcrystalline cellulose 120 mg Mannitol 100 mg Simeticone 20 mg Silicon dioxide 10 mg -
- Adsorb the simeticone on the mannitol and on part of the microcrystalline cellulose.
- Mix the resulting product with Phaseolus vulgaris dried extract, Salacia oblonga dried extract, the remainder of the microcrystalline cellulose and the silicon dioxide.
- Divide the mixture between hard gelatin capsules.
-
Unit composition: Phaseolus vulgaris dried extract 100 mg Salacia oblonga dried extract 150 mg Microcrystalline cellulose 150 mg Mannitol 100 mg Cross-linked sodium carboxymethylcellulose 30 mg Silicon dioxide 8 mg Magnesium stearate 5 mg -
- Mix the Phaseolus vulgaris dried extract, Salacia oblonga dried extract, microcrystalline cellulose, mannitol, crosslinked sodium carboxymethylcellulose and silicon dioxide for 10 minutes.
- Add the magnesium stearate to the mixture and proceed with mixing for a further 2 minutes.
- Compress the mixture obtained in the preceding point.
-
Unit composition: Phaseolus vulgaris extract 50 mg Salacia oblonga extract 100 mg Microcrystalline cellulose 100 mg Povidone 10 mg Sodium carboxymethylcellulose 8 mg Methacrylic acid copolymer 50 mg Triethyl citrate 3.2 mg Talc 8 mg Simeticone 0.3 mg -
- Granulate the extracts, microcrystalline cellulose and sodium carboxymethylcellulose with an aqueous solution of povidone.
- Dry and calibrate the granulate obtained.
- Coat the granules with an aqueous suspension of methacrylic acid copolymer, triethyl citrate, talc and simeticone.
-
Unit composition: Phaseolus vulgaris extract 50 mg Salacia oblonga extract 100 mg Microcrystalline cellulose 100 mg Povidone 10 mg Sodium carboxymethylcellulose 8 mg -
- Granulate the extracts, microcrystalline cellulose and sodium carboxymethylcellulose with an aqueous solution of povidone.
- Dry and calibrate the granulate obtained.
-
- Mix 50% of the granulate described in example 3 with 50% of the granulate described in example 4.
- Divide the mixture obtained between hard gelatin capsules.
Claims (4)
- Formulations consisting of (i) Phaseolus vulgaris extracts containing pharmacologically active quantities of α-amylase inhibitors and (ii) Salacia oblonga or Salacia reticulata containing pharmacologically active quantities of α-glucosidase inhibitors.
- Formulations as claimed in claim 1, wherein the Phaseolus vulgaris extract is characterised by an alpha-amylase inhibitor content of between 1200 and 1600 USP/mg (HPLC titre between 7 and 14% w/w) and a phytohaemagglutinin content of between 12,000 and 30,000 HAU/g.
- Formulations as claimed in claim 1 or 2, wherein the Phaseolus vulgaris and Salacia oblonga or Salacia reticulata extracts have a weight ratio of between 1:1 and 1:4.
- Use of the formulations claimed in claims 1-3 for the preparation of medicaments with a hypoglycaemic and anti-obesity action.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07425134A EP1967200A1 (en) | 2007-03-07 | 2007-03-07 | Formulations of alpha-amylase inhibitors of P. vulgaris with alpha-glucosidade inhibitores of S. oblonga or S. reticulata useful in the treatment of diabetes and obesity |
PCT/EP2008/001783 WO2008107182A2 (en) | 2007-03-07 | 2008-03-06 | Formulations of al pha-amylas e inhibitors of p. vulgaris with alpha-glucos idade inhibitores of s. oblonga or s. reticulata useful in the treatment of diabetes and obesity |
Applications Claiming Priority (1)
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EP07425134A EP1967200A1 (en) | 2007-03-07 | 2007-03-07 | Formulations of alpha-amylase inhibitors of P. vulgaris with alpha-glucosidade inhibitores of S. oblonga or S. reticulata useful in the treatment of diabetes and obesity |
Publications (1)
Publication Number | Publication Date |
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EP1967200A1 true EP1967200A1 (en) | 2008-09-10 |
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EP07425134A Withdrawn EP1967200A1 (en) | 2007-03-07 | 2007-03-07 | Formulations of alpha-amylase inhibitors of P. vulgaris with alpha-glucosidade inhibitores of S. oblonga or S. reticulata useful in the treatment of diabetes and obesity |
Country Status (2)
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EP (1) | EP1967200A1 (en) |
WO (1) | WO2008107182A2 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2397039A1 (en) * | 2010-06-21 | 2011-12-21 | Abbott Laboratories | Compositions for delaying progression of diabetes using Salacia oblonga extract |
CN103159839A (en) * | 2013-03-28 | 2013-06-19 | 云南天保桦生物资源开发有限公司 | Thermostability-protected alpha-amylase inhibitor and application thereof |
CN104857062A (en) * | 2015-06-09 | 2015-08-26 | 江南大学 | Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor |
CN108576735A (en) * | 2018-05-09 | 2018-09-28 | 北京市农林科学院 | It is a kind of that there is the jelly and preparation method thereof for inhibiting starch decomposition |
Citations (4)
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DE2628757A1 (en) * | 1976-06-26 | 1977-12-29 | Guenter Dr Woeber | Amylase inhibitor from Phaseolus vulgaris - active against amylase from saliva or pancreas, does not inhibit alpha-amylase from e.g. Bacillus subtilis |
EP1295535A2 (en) * | 2001-09-25 | 2003-03-26 | Pharmachem Laboratories, Inc. | Phaseolamin compositions and methods for using the same |
WO2004002239A2 (en) * | 2002-06-28 | 2004-01-08 | Pharmachem Laboratories, Inc. | Purified amylase inhibitor and novel process for obtaining the same |
WO2005094602A2 (en) * | 2004-03-30 | 2005-10-13 | Consiglio Nazionale Delle Ricerche | Purified extract of an alpha-amylase inhibitor from phytoemagglutinin-essentially free beans, process for its extraction and compositions containing it |
-
2007
- 2007-03-07 EP EP07425134A patent/EP1967200A1/en not_active Withdrawn
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2008
- 2008-03-06 WO PCT/EP2008/001783 patent/WO2008107182A2/en active Application Filing
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DE2628757A1 (en) * | 1976-06-26 | 1977-12-29 | Guenter Dr Woeber | Amylase inhibitor from Phaseolus vulgaris - active against amylase from saliva or pancreas, does not inhibit alpha-amylase from e.g. Bacillus subtilis |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2397039A1 (en) * | 2010-06-21 | 2011-12-21 | Abbott Laboratories | Compositions for delaying progression of diabetes using Salacia oblonga extract |
WO2011163183A3 (en) * | 2010-06-21 | 2012-02-16 | Abbott Laboratories | Methods for delaying progression of diabetes using salacia oblonga extract |
CN103159839A (en) * | 2013-03-28 | 2013-06-19 | 云南天保桦生物资源开发有限公司 | Thermostability-protected alpha-amylase inhibitor and application thereof |
CN103159839B (en) * | 2013-03-28 | 2014-07-30 | 云南天保桦生物资源开发有限公司 | Thermostability-protected alpha-amylase inhibitor and application thereof |
CN104857062A (en) * | 2015-06-09 | 2015-08-26 | 江南大学 | Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor |
CN104857062B (en) * | 2015-06-09 | 2018-08-21 | 无锡华康生物科技有限公司 | A kind of industrialized process for preparing of navy bean alpha-amylase inhibitor |
CN108576735A (en) * | 2018-05-09 | 2018-09-28 | 北京市农林科学院 | It is a kind of that there is the jelly and preparation method thereof for inhibiting starch decomposition |
Also Published As
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WO2008107182A2 (en) | 2008-09-12 |
WO2008107182A3 (en) | 2009-08-13 |
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