EP1920253A2 - Detection de proteases secretees a partir de micro-organismes pathogenes - Google Patents
Detection de proteases secretees a partir de micro-organismes pathogenesInfo
- Publication number
- EP1920253A2 EP1920253A2 EP06789202A EP06789202A EP1920253A2 EP 1920253 A2 EP1920253 A2 EP 1920253A2 EP 06789202 A EP06789202 A EP 06789202A EP 06789202 A EP06789202 A EP 06789202A EP 1920253 A2 EP1920253 A2 EP 1920253A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- protease
- substrate
- reagent
- test sample
- color
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
- G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
Definitions
- the diazonium ion may be zwitterionic in that the counterion of the diazonium moiety is covalently bound to the ring system.
- the ring system of the diazonium ion may be substituted or unsubstituted.
- the ion may be provided by a variety of suitable diazonium salts, such as diazonium chlorides, diazonium acid sulphates, diazonium alkyl sulphates, diazonium fluoborates, diazonium benzenesulphonates, diazonium acid 1 ,5-naphthalenedisulphonates, and so forth.
- a substrate is employed.
- the substrate may be mixed with the test sample prior to application to the device 20.
- the substrate may be diffusively immobilized on the device 20 prior to application of the test sample.
- Such pre-application provides a variety of benefits, including the elimination of the need for a subsequent user to handle and mix the reagents with the test sample or a diluent. This is particularly useful in point-of- care applications when the user is not generally a trained lab technician or medical professional.
- the substrate may be disposed downstream from the sample application zone 24. In this manner, the test sample is capable of mixing with the substrate upon application. Alternatively, the substrate may be positioned upstream from the sample application zone 24. For instance, a diluent may be employed to induce mixing between the substrate and test sample.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La présente invention a trait à une trousse d'essai diagnostique pour la détection de la présence ou de l'absence d'une protéase (par exemple, l'aminopeptidase) au sein d'un échantillon d'essai. La trousse d'essai comporte un substrat qui est capable d'être clivé en présence de la protéase pour la libération d'un composé. La trousse comporte également un dispositif d'écoulement latéral comprenant un support chromatographique. Le support chromatographique définit une zone de détection au sein de laquelle est contenu un premier réactif (par exemple, l'ion diazonium) qui est capable de réagir avec le composé pour former un deuxième réactif (par exemple, un composé azoïque aromatique). Le deuxième réactif présente une couleur qui est différente de la couleur du premier réactif. Le dispositif d'écoulement latéral comporte également un matériau absorbant disposé adjacent au support chromatographique, le matériau absorbant recevant l'échantillon d'essai suite à son écoulement à travers le support chromatographique.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/217,097 US7504235B2 (en) | 2005-08-31 | 2005-08-31 | Enzyme detection technique |
| US11/364,810 US7575887B2 (en) | 2005-08-31 | 2006-02-28 | Detection of proteases secreted from pathogenic microorganisms |
| PCT/US2006/030107 WO2007027353A2 (fr) | 2005-08-31 | 2006-08-01 | Detection de proteases secretees a partir de micro-organismes pathogenes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1920253A2 true EP1920253A2 (fr) | 2008-05-14 |
Family
ID=37809345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP06789202A Withdrawn EP1920253A2 (fr) | 2005-08-31 | 2006-08-01 | Detection de proteases secretees a partir de micro-organismes pathogenes |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP1920253A2 (fr) |
| WO (1) | WO2007027353A2 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7504235B2 (en) * | 2005-08-31 | 2009-03-17 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
| US7935538B2 (en) | 2006-12-15 | 2011-05-03 | Kimberly-Clark Worldwide, Inc. | Indicator immobilization on assay devices |
| US20080269707A1 (en) | 2007-04-30 | 2008-10-30 | Kimberly-Clark Worldwide, Inc. | Lateral Flow Device for Attachment to an Absorbent Article |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999027364A1 (fr) * | 1997-11-24 | 1999-06-03 | Quidel Corporation | Mise en place du substrat enzymatique et enregistrement du produit dans des dosages immunoenzymatiques en une etape |
| WO2006006961A1 (fr) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Detection d'enzymes |
| WO2006006960A1 (fr) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Detection d'enzymes et d'amines en une seule operation |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3413077A1 (de) * | 1984-04-06 | 1985-10-17 | Miles Laboratories, Inc., Elkhart, Ind. | Chromogene aminosaeure- und peptidester, verfahren zu deren herstellung, verwendung dieser verbindungen in analysenverfahren sowie mittel zum nachweis esterolytischer und/oder proteolytischer enzyme |
| US5571684A (en) * | 1994-11-07 | 1996-11-05 | Litmus Concepts, Inc. | Assay for proline iminopeptidase and other hydrolytic activities |
| US7781172B2 (en) * | 2003-11-21 | 2010-08-24 | Kimberly-Clark Worldwide, Inc. | Method for extending the dynamic detection range of assay devices |
| US20050220712A1 (en) * | 2004-04-01 | 2005-10-06 | Wright Daniel G | Method for determining mucosal neutrophil counts in neutropenia patents |
| US7504235B2 (en) * | 2005-08-31 | 2009-03-17 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
-
2006
- 2006-08-01 EP EP06789202A patent/EP1920253A2/fr not_active Withdrawn
- 2006-08-01 WO PCT/US2006/030107 patent/WO2007027353A2/fr active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999027364A1 (fr) * | 1997-11-24 | 1999-06-03 | Quidel Corporation | Mise en place du substrat enzymatique et enregistrement du produit dans des dosages immunoenzymatiques en une etape |
| WO2006006961A1 (fr) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Detection d'enzymes |
| WO2006006960A1 (fr) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Detection d'enzymes et d'amines en une seule operation |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007027353A3 (fr) | 2007-06-07 |
| WO2007027353A2 (fr) | 2007-03-08 |
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