EP1716405A1 - Dispositif portatif permettant de mesurer la concentration totale de gossypol - Google Patents

Dispositif portatif permettant de mesurer la concentration totale de gossypol

Info

Publication number
EP1716405A1
EP1716405A1 EP03780426A EP03780426A EP1716405A1 EP 1716405 A1 EP1716405 A1 EP 1716405A1 EP 03780426 A EP03780426 A EP 03780426A EP 03780426 A EP03780426 A EP 03780426A EP 1716405 A1 EP1716405 A1 EP 1716405A1
Authority
EP
European Patent Office
Prior art keywords
gossypol
light
cuvettee
concentration
voltage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP03780426A
Other languages
German (de)
English (en)
Inventor
Madan Lal c/o Metallurgy Division SINGLA
Mewa c/o Metallurgy Division SINGH
Prasant K. c/o Metallurgy Division MAHAPATRA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council of Scientific and Industrial Research CSIR
Original Assignee
Council of Scientific and Industrial Research CSIR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council of Scientific and Industrial Research CSIR filed Critical Council of Scientific and Industrial Research CSIR
Publication of EP1716405A1 publication Critical patent/EP1716405A1/fr
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions

Definitions

  • the present invention relates to a device useful for the determination of Gossypol in Cottonseed deoiled cake, cottonseed and compounds having gossypol after development of green colour complex with Iron [Fe (III)]. Background and Prior arts
  • Cottonseed products in the forms of oils and meal are widely used for human and animal food.
  • the utilization of cottonseed products in human and its nutrition is limited by the presence of polyphenolic compounds, Gossypol.
  • Gossypol polyphenolic compounds
  • the toxicity of gossypol also limits to own application in many areas.
  • Gossypol is a biologically active terpenoid substance present in discrete glands in many parts of the cotton plant, including the seed kernel. Much of the gossypol is found naturally in the glands or chemically bound to the protein portion of the seed. The pigment is a toxic compound in its free form and accumulates in body tissues. Cottonseed is processed into oils and meals for both animal and human consumption. Toxicity of gossypol is attributed to free gossypol, as opposed to bound gossypol. Food and Drug Administration (FDA) and the United Nations Protein Advisory Group established limits for gossypol content in human foodstuffs.
  • FDA Food and Drug Administration
  • FDA Food and Drug Administration
  • Protein Advisory Group established limits for gossypol content in human foodstuffs.
  • Methods used for analysis of gossypol includes spectrophotometric method (American Oil Chemist Society” Official and Tentative Methods", American Oil Chemists Society: Champaign, IL, 1978; Tentative Method Ba 8-58; Crouch, Floyd W; Bryant Melton F. Anal. Chem. 1982,54,242-246; Pons, W. A.; Guthrie, J. D. J. Am. Oil Chem. Soc. 1949,26,671-676, Pons, W. A.; Hoffpauir C. L. ;O'Connor, R. T. J. Am. Oil Chem. Soc 1951 ,28, 8-12.) for the determination of gossypol using available spectrophotometers.
  • the novelty of the present invention is that concentration of total gossypol is directly displayed on LCD in mg I "1 or ppm from the test solution and no calibration curve is needed for measuring the absorbance of solutions containing known amount of gossypol.
  • the background of the art clearly shows the drawbacks in the prior arts and the inventors have studied comprehensively and identified the limitations involved in the above-mentioned processes.
  • the inventors have devised an apparatus for directly displaying on LCD in mgl "1 or ppm from the test solution and no calibration curve is needed.
  • individually the function of the components involved in the apparatus are known but the assembling and functional inter-relationship to achieve a desired result have been obtained for the first time.
  • the inventive and novel features of the invention have been marked out.
  • the main objective of the present invention is to provide a portable system for the measurement of total gossypol in cottonseeds, cottonseed-deoiled cake, cottonseed oil and in gossypol compound after green colour development.
  • the other objective of the present invention is to provide a portable device useful for the determination of gossypol in deoiled cake, cottonseed in terms of milligram litre -
  • LED source of 635 nm (50 milliwatt) thus consuming lower power eliminating the use of tungsten lamp, filters.
  • Another object of invention is to eliminate the cumbersome optics in spectrophotometer. Another objective is that no calibration curve is to stored in memory for display of actual values of gossypol.
  • Another object of the present invention is that 100% transmittance level in distilled water is adjusted from the intensity of the light.
  • sample holder is especially designed to hold Borosil circular Glass cuvettee of 17 mm dia with volume capacity of 6 ml.
  • Still another Objective of the present invention is that the system operates at ⁇ 6 V (
  • the device of the present invention is a useful as a prototype instrument to measure gossypol concentration in deoiled cake/cottonseed/cottonseed oil.
  • the instrument of the present invention is used on the Lambert Beer's Law.
  • Figure 1 Projected views of the cuvette sample holder
  • Figure 2 represents the block diagram of the device
  • Figure 3 represents the block diagram of the sample holder
  • the device of the present invention comprises of following sub-system.
  • Fig. (I) of the drawing accompanying this specifications the Block diagram of the device of the present invention is shown.
  • the intensity of light from LED has been adjusted with a potentiometer (rnultitum) in order to get 100% transmittance of light through distilled water in a cuvettee.
  • According to the present invention provides a useful device for the determination of gossypol in cottonseed, cottonseed meals in terms of milligram/litre or parts per million (ppm).
  • the device used 5 mm size light emitting diode (LED) size of 635 nm with a adjustable potentiometer (multiturn) in order to get 100% transmittance or 0% absorbance of light through distilled water in the glass cuvettee of 17 mm path length.
  • the cuvettee holder is made up from aluminium alloy which has been blackened.
  • the cuvettee holder has two sub-system tilted at opposite ends, one hold LED and other hold the detector.
  • the cuvettee used is made up from Borosil glass with dia 17 mm, height is 5 cm and capacity of 6 ml with screw capped.
  • a photo-detector BPW 21 having package of TO 5, effective area of detection of 5.9 mm diameter wavelength range of 460-750 nm and sensitivity of 7nA lux is used to detect the transmitted / absorbed light through the test solution.
  • the detected light signal by the photo detector is converted to voltage using a current to voltage converter. The said current is proportional to the light signal detected.
  • the output voltage of the l/V converter is given by the potential difference between the resistance R3 and R4 as shown in figure 2.
  • the log amplifier is TL 441 IC.
  • voltage output after, log amplification digit has been calibrated in terms of gossypol concentration in test solution.
  • VisiDAQ software for data acquisition S. calculation after ADAM acquisition Module whose input is analog voltage derived from l-V converter & output is in digital form, which is connected to serial port of PC.
  • VisiDAQ is data acquisition and control application software and also an application builder for all kinds of automation.
  • the script designer is a text editor with some convenient features for editing script code.
  • the script source will be compiled into p- code after editing so it won't need to be compiled again at run-time.
  • the syntax of the Basic Script is compatible with Microsoft VBA (Visual Basic for Application in Excel, Word, Access, etc.) and Microsoft Visual Basic.
  • Task Designer uses a dataflow-programming model that frees user from the linear architecture of text-based languages. To create a process monitoring and control application, user construct the block diagram without worrying about the many syntactical details of conventional programming. Simply select objects (icons) from the Functions menu and connect them with wires to pass data from one block to the next. Task Designer allows editing of multiple tasks at the same time. Each task is contained in a task window and has it's own properties such as scan rate, start/stop method, etc.
  • One strategy file is used to store all scan tasks that are related to a control strategy. For simple strategy with only one task, it runs the same way as before. But for strategies that have more than one scan task, a top-level main script is required to manage the execution of all scan tasks. A large complicated task can be broken into several smaller, simpler tasks. This not only simplifies the editing job, but also increases the performance at run-time, as fewer blocks need to be processed at each scan.
  • the Virtual Tag is a powerful feature that provides the ability to let developer to create customized tag in Task Designer without using User Defined DLL.
  • the virtual tag is created by Task Designer and stored in data center as other built-in blocks.
  • the virtual tags are global available to all tasks, so you can use virtual tags to share data among multiple tasks.
  • Data collection function creates database files for each defined TAG point at a user- specified time. Data collection function is designed for report generation. The shortest time interval for data collection from a given TAG point is 10 minutes. High-speed data collection is accomplished through other trend data collection functions.
  • ADVANTAGES The main advantage of the present invention: ⁇ It is Low cost and portable system ⁇
  • the sample holder is especially designed to hold glass cuvettee of 17 mm dia ⁇
  • the concentration of gossypol is directly displayed in ppm level after the colour development » No standard solution is required to make calibration curve.

Landscapes

  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Measuring Cells (AREA)

Abstract

La mesure du gossypol dans les produits à base de graines de coton à l'aide de méthodes traditionnelles, à savoir les méthodes spectrophotométriques et à résonance magnétique nucléaire, les méthodes par chimiluminescence, la polarographie par chromatographie gaz-liquide, la chromatographie sur couche mince et la chromatographie sur papier, est pénible, lente et onéreuse et elle exige des systèmes étalonnés. La présente invention propose un système portatif et peu coûteux permettant d'afficher directement sur un écran à cristaux liquides la concentration de gossypol total en mgl-1 ou en ppm dans la solution testée, et aucune solution témoin n'est nécessaire à l'établissement d'une courbe d'étalonnage pour la mesure de l'absorbance.
EP03780426A 2003-12-16 2003-12-16 Dispositif portatif permettant de mesurer la concentration totale de gossypol Ceased EP1716405A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2003/005953 WO2005062023A1 (fr) 2003-12-16 2003-12-16 Dispositif portatif permettant de mesurer la concentration totale de gossypol

Publications (1)

Publication Number Publication Date
EP1716405A1 true EP1716405A1 (fr) 2006-11-02

Family

ID=34708466

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03780426A Ceased EP1716405A1 (fr) 2003-12-16 2003-12-16 Dispositif portatif permettant de mesurer la concentration totale de gossypol

Country Status (5)

Country Link
EP (1) EP1716405A1 (fr)
JP (1) JP2007527499A (fr)
CN (1) CN1910443A (fr)
AU (1) AU2003288585A1 (fr)
WO (1) WO2005062023A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101806771B (zh) * 2010-04-14 2013-09-25 成都理工大学 一种极谱仪前置放大电路装置及方法
FR2989464A1 (fr) * 2012-04-12 2013-10-18 Agronomique Inst Nat Rech Procede et dispositif de mesure d'etat de reaction chimique ou biologique

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1559810A (en) * 1975-11-05 1980-01-30 Nat Res Dev Device for measuring light received from an illuminated material
US4357105A (en) * 1980-08-06 1982-11-02 Buffalo Medical Specialties Mfg., Inc. Blood diagnostic spectrophotometer
JPH08159954A (ja) * 1994-12-07 1996-06-21 Tosoh Corp グリコヘモグロビン測定用の吸光度計
US6261848B1 (en) * 1998-05-08 2001-07-17 The Johns Hopkins University Miniature immuno-optical rapid analyte sensor platform

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005062023A1 *

Also Published As

Publication number Publication date
WO2005062023A1 (fr) 2005-07-07
JP2007527499A (ja) 2007-09-27
CN1910443A (zh) 2007-02-07
AU2003288585A1 (en) 2005-07-14

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