EP1572981A4 - Mutations affectant le nombre de copies de plasmides - Google Patents

Mutations affectant le nombre de copies de plasmides

Info

Publication number
EP1572981A4
EP1572981A4 EP03800442A EP03800442A EP1572981A4 EP 1572981 A4 EP1572981 A4 EP 1572981A4 EP 03800442 A EP03800442 A EP 03800442A EP 03800442 A EP03800442 A EP 03800442A EP 1572981 A4 EP1572981 A4 EP 1572981A4
Authority
EP
European Patent Office
Prior art keywords
copy number
plasmid copy
mutations affecting
affecting plasmid
mutations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03800442A
Other languages
German (de)
English (en)
Other versions
EP1572981A1 (fr
Inventor
Qiong Cheng
Pierre E Rouviere
Wonchul Suh
Luan Tao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of EP1572981A1 publication Critical patent/EP1572981A1/fr
Publication of EP1572981A4 publication Critical patent/EP1572981A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP03800442A 2002-12-20 2003-12-19 Mutations affectant le nombre de copies de plasmides Withdrawn EP1572981A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43497302P 2002-12-20 2002-12-20
US434973P 2002-12-20
PCT/US2003/041809 WO2004058951A1 (fr) 2002-12-20 2003-12-19 Mutations affectant le nombre de copies de plasmides

Publications (2)

Publication Number Publication Date
EP1572981A1 EP1572981A1 (fr) 2005-09-14
EP1572981A4 true EP1572981A4 (fr) 2005-12-28

Family

ID=32682132

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03800442A Withdrawn EP1572981A4 (fr) 2002-12-20 2003-12-19 Mutations affectant le nombre de copies de plasmides

Country Status (5)

Country Link
US (1) US7291482B2 (fr)
EP (1) EP1572981A4 (fr)
AU (1) AU2003300193A1 (fr)
CA (1) CA2509702A1 (fr)
WO (1) WO2004058951A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7247467B2 (en) * 2003-09-17 2007-07-24 E. I. Du Pont De Nemours And Company Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number
AU2006281994B2 (en) * 2005-08-19 2011-10-06 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
JP2009017842A (ja) * 2007-07-13 2009-01-29 Japan Agengy For Marine-Earth Science & Technology 生産性改善法
JP2009017840A (ja) * 2007-07-13 2009-01-29 Japan Agengy For Marine-Earth Science & Technology 外来遺伝子を細胞に安定に保持する方法
US20130266983A1 (en) * 2010-12-14 2013-10-10 The Regents Of The University Of California Creation of super-stable ColE1 plasmids by duplication of SL1-4 sequence and point mutations.
EP3699592A1 (fr) * 2013-03-13 2020-08-26 Geneweave Biosciences Inc. Particules de transduction non réplicative et systèmes de rapporteur basé sur des particules de transduction
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
US11077444B2 (en) 2017-05-23 2021-08-03 Roche Molecular Systems, Inc. Packaging for a molecular diagnostic cartridge
KR102624718B1 (ko) * 2018-08-07 2024-01-12 씨제이제일제당 주식회사 변이형 rpoc 암호화 서열을 포함하는 핵산 분자
US11549116B2 (en) * 2018-08-07 2023-01-10 Cj Cheiljedang Corporation Nucleic acid molecules comprising a variant inc coding strand

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004056974A2 (fr) * 2002-12-19 2004-07-08 E.I. Du Pont De Nemours And Company Mutations affectant la production de carotenoides
WO2004056975A2 (fr) * 2002-12-19 2004-07-08 E.I. Du Pont De Nemours And Company Augmentation de la production de carotenoides dans des bacteries par integration chromosomique

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7220571B2 (en) 2000-09-28 2007-05-22 Archer-Daniels-Midland Company Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004056974A2 (fr) * 2002-12-19 2004-07-08 E.I. Du Pont De Nemours And Company Mutations affectant la production de carotenoides
WO2004056975A2 (fr) * 2002-12-19 2004-07-08 E.I. Du Pont De Nemours And Company Augmentation de la production de carotenoides dans des bacteries par integration chromosomique

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EDERTH J ET AL: "Origin-specific reduction of ColE1 plasmid copy number due to mutations in a distinct region of the Escherichia coli RNA polymerase", MGG MOLECULAR GENETICS AND GENOMICS, vol. 267, no. 5, July 2002 (2002-07-01), pages 587 - 592, XP002352297, ISSN: 1617-4615 *
See also references of WO2004058951A1 *

Also Published As

Publication number Publication date
CA2509702A1 (fr) 2004-07-15
US20040191863A1 (en) 2004-09-30
US7291482B2 (en) 2007-11-06
AU2003300193A1 (en) 2004-07-22
EP1572981A1 (fr) 2005-09-14
WO2004058951A1 (fr) 2004-07-15

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20050606

AK Designated contracting states

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

STAA Information on the status of an ep patent application or granted ep patent

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A4 Supplementary search report drawn up and despatched

Effective date: 20051114

18W Application withdrawn

Effective date: 20051209