EP1563097A4 - Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification - Google Patents

Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification

Info

Publication number
EP1563097A4
EP1563097A4 EP03783738A EP03783738A EP1563097A4 EP 1563097 A4 EP1563097 A4 EP 1563097A4 EP 03783738 A EP03783738 A EP 03783738A EP 03783738 A EP03783738 A EP 03783738A EP 1563097 A4 EP1563097 A4 EP 1563097A4
Authority
EP
European Patent Office
Prior art keywords
amplification
amplification reaction
quantitative
sampling method
reaction analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03783738A
Other languages
German (de)
English (en)
Other versions
EP1563097A2 (fr
Inventor
Vladimir I Slepnev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PRIMERA BIOSYSTEMS
Original Assignee
PRIMERA BIOSYSTEMS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PRIMERA BIOSYSTEMS filed Critical PRIMERA BIOSYSTEMS
Publication of EP1563097A2 publication Critical patent/EP1563097A2/fr
Publication of EP1563097A4 publication Critical patent/EP1563097A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés et dispositifs d'échantillonnage permettant d'analyser quantitativement des réactions d'amplification d'acide nucléique. Des informations quantitatives recueillies lors du déroulement d'un protocole d'amplification permettent de développer un profil d'amplification détaillé qui permet, à son tour, de déterminer de manière fiable la quantité de matrices initiales. Les procédés selon l'invention peuvent en particulier être utilisés pour analyser quantitativement l'expression de gènes ou unités de transcription multiples, non seulement lors de la même réaction d'amplification, mais aussi au cours de réactions d'amplification multiples.
EP03783738A 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification Withdrawn EP1563097A4 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US42803802P 2002-11-21 2002-11-21
US428038P 2002-11-21
US44001003P 2003-01-14 2003-01-14
US43998203P 2003-01-14 2003-01-14
US440010P 2003-01-14
US439982P 2003-01-14
PCT/US2003/037420 WO2004048528A2 (fr) 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification

Publications (2)

Publication Number Publication Date
EP1563097A2 EP1563097A2 (fr) 2005-08-17
EP1563097A4 true EP1563097A4 (fr) 2006-03-15

Family

ID=32397974

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03783738A Withdrawn EP1563097A4 (fr) 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification

Country Status (6)

Country Link
EP (1) EP1563097A4 (fr)
JP (1) JP2006507849A (fr)
KR (1) KR20050075436A (fr)
AU (1) AU2003291144A1 (fr)
CA (1) CA2509769A1 (fr)
WO (1) WO2004048528A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7445893B2 (en) 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
JP4981295B2 (ja) * 2005-10-04 2012-07-18 キヤノン株式会社 Ｄｎａ処理方法およびｄｎａ処理装置
EP2021113A2 (fr) * 2006-05-11 2009-02-11 Raindance Technologies, Inc. Dispositifs microfluidiques
DE102013200193A1 (de) 2013-01-09 2014-07-10 Hamilton Bonaduz Ag Probenverarbeitungssystem mit Dosiervorrichtung und Thermocycler
KR101816288B1 (ko) * 2016-08-11 2018-01-09 한국기초과학지원연구원 고흡수성 수지를 이용한 시료의 전처리 방법 및 이를 위한 디바이스
JP7090256B1 (ja) 2022-01-14 2022-06-24 ヨダカ技研株式会社 温度調整装置、駆動装置、遺伝子増幅システム、遺伝子増幅方法、及び遺伝子増幅プログラム

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998033942A1 (fr) * 1997-01-30 1998-08-06 University Technology Corporation Diagnostic et traitement de defaillance myocardique
WO2000066995A2 (fr) * 1999-04-29 2000-11-09 Genome Therapeutics Corporation Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification
WO2001055454A1 (fr) * 2000-01-28 2001-08-02 Althea Technologies, Inc. Procedes d'analyse de l'expression genique
WO2002016652A2 (fr) * 2000-08-25 2002-02-28 Genome Therapeutics Corporation Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides
WO2003035841A2 (fr) * 2001-10-24 2003-05-01 Sention, Inc Procedes et systemes de profilage dynamique de l'expression de genes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
CA2304260C (fr) * 1999-04-20 2009-03-24 Japan Bioindustry Association Methode pour determiner la concentration de molecules cibles d'acides nucleiques, capteurs a acides nucleiques pour cette methode et methode pour analyser les donnees ainsi obtenues
US6531282B1 (en) * 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci
US6482615B2 (en) * 2001-03-02 2002-11-19 Integrated Genetic Devices Ltd. Method and apparatus for effecting rapid thermal cycling of samples in microtiter plate size

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998033942A1 (fr) * 1997-01-30 1998-08-06 University Technology Corporation Diagnostic et traitement de defaillance myocardique
WO2000066995A2 (fr) * 1999-04-29 2000-11-09 Genome Therapeutics Corporation Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification
WO2001055454A1 (fr) * 2000-01-28 2001-08-02 Althea Technologies, Inc. Procedes d'analyse de l'expression genique
WO2002016652A2 (fr) * 2000-08-25 2002-02-28 Genome Therapeutics Corporation Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides
WO2003035841A2 (fr) * 2001-10-24 2003-05-01 Sention, Inc Procedes et systemes de profilage dynamique de l'expression de genes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DORSCHNER ET AL: "Diagnosis of five spinocerebellar ataxia disorders by multiplex amplification and capillary electrophoresis", JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 4, no. 2, May 2002 (2002-05-01), pages 108 - 113 *
GARCIA ELIZABETH P ET AL: "Scalable transcriptional analysis routine - Multiplexed quantitative real-time polymerase chain reaction platform for gene expression analysis and molecular diagnostics", JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 7, no. 4, October 2005 (2005-10-01), pages 444 - 454, XP009060044, ISSN: 1525-1578 *
LI ET AL: "Quantitative polymerase chain reaction using capillary electrophoresis with laser-induced fluorescence detection: Analysis of duck hepatitis B", ANAL.BIOANAL.CHEM, vol. 374, 28 August 2002 (2002-08-28), pages 269 - 273 *
MELDRUM DEIRDRE: "Automation for genomics, Part One: Preparation for sequencing", GENOME RESEARCH, vol. 10, no. 8, August 2000 (2000-08-01), pages 1081 - 1092, XP000958747, ISSN: 1088-9051 *
WIESNER R J ET AL: "COUNTING TARGET MOLECULES BY EXPONENTIAL POLYMERASE CHAIN REACTION:COPY NUMBER OF MITOCHONDRIAL DNA IN RAT TISSUES", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 183, no. 2, 15 March 1992 (1992-03-15), pages 553 - 559, XP000561778, ISSN: 0006-291X *
WIESNER R J: "DIRECT QUANTIFICATION OF PICOMOLAR CONCENTRATIONS OF MRNAS BY MATHEMATICAL ANALYSIS OF A REVERSE TRANSCRIPTION/EXPONENTIAL POLYMERASE CHAIN REACTION ASSAY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 21, 11 November 1992 (1992-11-11), pages 5863 - 5864, XP000561191, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2003291144A1 (en) 2004-06-18
WO2004048528A3 (fr) 2004-11-11
WO2004048528A2 (fr) 2004-06-10
EP1563097A2 (fr) 2005-08-17
CA2509769A1 (fr) 2004-06-10
JP2006507849A (ja) 2006-03-09
KR20050075436A (ko) 2005-07-20

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