PROCESS FOR PURIFYING AND ISOLATING RAC-BICALUTA IDE
FIELD OF THE INVENTION The present invention relates to a process for isolating røc-bicalutamide and its intermediates.
BACKGROUND OF THE INVENTION Bicalutamide is also known as N-[4-cyano-3-trifluoromethyl-phenyl]-3-[4- fluorophenyl-sulfonyl]-2-hydroxy-2-methyl-propionamide and has the following chemical formula.
Bicalutamide
Bicalutamide is an acylanilid that has anti-androgen activity. It is known to selectively decrease the testosterone level without influencing the regulation mechanisms of the hypothalamus. The international patent No. WO 93/19770 describes both R-(-) enantiomer and S-
(+) enantiomer for bicalutamide, of which the R-(-) isomer is reported to be more active and possesses lesser side-effects (e.g., headache, gynecomistia and giddiness) when used in therapy treatment. U.S. Pat. No. 4,636,505 describes processes for preparing acylanilides.
The international patent No. WO 01/00608 describes a process for racemic and optically pure N-[4-cyano-3-trifluoromethylphenyl]-3-[4-fluorophenyl-sulfonyl]-2-
hydroxy-2-methyl-propionamide. The process involves multiple steps including at least reacting with thionyl choride; hydrolyzing under aqueous basic conditions; sulfonylating with sulfonyl halogenide; and oxidizing with inorganic peroxy salt or m-chloroperbenzoic acid (MCPBA) or aqueous hydrogen peroxide. However, the synthetic pathways involve the use of substrates (such as sodium hydride) that are dangerously explosive in nature.
There is a constant need to improve the synthesis process for bicalutamide which are economical and environmental safe and feasible. One approach to address this need is described in pending U.S. application serial no. 10/170,721 which describes the preparation of rac-bicalutarnide, intermediates and derivatives thereof from
4-fluorophenyl methyl sulfone and 5-amino-2-cyano-benzotrifmoride precursors.
We have now found a novel, safe and effective method for the purification and isolation of bicalutamide.
OBJECTS AND SUMMARY OF THE INVENTION The present invention provides a process for the purification and crystallization of rαc-bicalutamide and its intermediates. According to one object, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining crude bicalutamide and a solvent;
(ii) crystallizing the bicalutamide from the solvent with or without seeding; and (iii) collecting the crystals of bicalutamide.
The crude bicalutamide may or may not be substantially soluble in the solvent. Preferably the crude bicalutamide is soluble in the solvent. The resulting bicalutamide solution or suspension is crystallized by applying agitation for a time sufficient to bring about crystallization of the bicalutamide.
Preferably, in this embodiment, the solvent is selected from the group consisting of water, methanol, ethanol, DCM, toluene, PE, chloroform, hexane, 1,2-dichloroethane, diethyl ether, propanol and isopropanol. According to another object, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining crude bicalutamide and a first solvent; (ii) adding a second solvent to the crude bicalutamide-first solvent mixture; (iii) crystallizing the bicalutamide from the solvents; and (iv) collecting the crystals of bicalutamide.
In one embodiment, the first solvent and the second solvent are the same. Preferably, in this embodiment, the crude bicalutamide is dissolved in an amount of first solvent sufficient to dissolve the bicalutamide. Preferably the resulting bicalutamide solution is heated to about the boiling point of the first solvent.
Preferably, in this embodiment, the amount of second solvent added to the bicalutamide solution is equal to that of the first volume. The second solvent is preferably added under reflux conditions.
Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25°C.
Preferably, in this embodiment, the first and second solvents are selected from the group consisting of ethyl acetate, acetonitrile, acetone, THF, propanol, DMF, DMSO and isobutyl methyl ketone. In another embodiment, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps
of:
(i) combining crude bicalutamide and a first solvent;
(ii) adding a second solvent to the crude bicalutamide-first solvent mixture, wherein the second solvent is an anti-solvent; (iii) crystallizing the bicalutamide from the solvents; and (iv) collecting the crystals of bicalutamide.
Preferably, in this embodiment, the crude bicalutamide is dissolved in an amount of first solvent sufficient to dissolve the bicalutamide. Preferably the resulting bicalutamide solution is heated to about the boiling point of the first solvent. Preferably, in this embodiment, the addition of the second solvent, or anti-solvent, takes place under reflux conditions, with the second solvent being added in an amount sufficient to bring about an at least partially desolubilized bicalutamide. Preferably, in this embodiment, following addition of the second solvent, a small volume of first solvent, sufficient to dissolve the at least partially desolubilized bicalutamide is added to the mixture.
Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25°C.
Preferably, in this embodiment, first solvent: second solvent system combinations include DMF:water and ethyl acetate:hexane.
In another embodiment, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining crude bicalutamide and a first solvent, wherein the first solvent is an anti- solvent;
(ii) adding a second solvent to the crude bicalutamide-first solvent mixture; (iii) crystallizing the bicalutamide from the solvents; and (iv) collecting the crystals of bicalutamide. Preferably, in this embodiment, the crude bicalutamide is adedd to the first solvent, or anti-solvent, and the resulting bicalutamide suspension is heated to about the boiling point of the first solvent. Preferably, in this embodiment, the addition of the second solvent, or anti-solvent, takes place under reflux conditions, with the second solvent being added in an amount sufficient to dissolve the bicalutamide.
Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25°C. Preferably, in this embodiment, the first solvent, or anti-solvent is selected from the group consisting of toluene, ether, chloroform, water, methanol and ethanol.
Preferably, in this embodiment, the second solvent is selected from the group consisting of acetonitrile, acetone, THF, DMF and isobutyl methyl ketone.
According to another object, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining bicalutamide and a first solvent; (ii) adding a second solvent to the bicalutamide-first solvent mixture;
(iii) crystallizing the bicalutamide from the solvents with or without seeding; and
(iv) collecting the crystals of bicalutamide.
According to another object, the present invention provides a process for the purification and isolation of bicalutamide by solution crystallization, wherein the solvents utilized have low toxic potential.
The present invention also provides rac-bicalutamide and its intermediates prepared and isolated by the processes described above.
DETAILED DESCRIPTION OF THE INVENTION Definitions: As used herein, rac-bicalutamide refers to both the R-(-) enantiomer and S-(+) enantiomer of bicalutamide. Rac-bicalutamide is the racemic and optically pure R-(-) and S-(+) isomers of N- [4-cyano-3 -trifluoromethyl-phenyl] -3 -[4-fluorophenyl-sulfonyl] -2- hydroxy-2-methyl-propionamide. It is to be understood that this invention encompasses the racemic form of bicalutamide and any optically-active form which possesses anti- androgenic activity. It is a matter of common general knowledge how a racemic compound may be resolved into its optically-active forms and how any anti-androgenic activity present in any of these forms may be determined. One skilled in the art will appreciate that the separation of optical isomers can be achieved by conventional resolution; such as fractional crystallization or flash-chromatography.
As used herein, the term "anti-solvent" refers to a solvent in which bicalutamide has limited or no solubility. As used herein, the term "crude bicalutamide" refers to the product prepared by a process to prepare bicalutamide.
The following abbreviations are used herein: DCM is dichloromethane. THF is tetrahydrofuran. DABCO is 1,4 dizazbicyl [2.2.2] octane. ACB is 5-amino-2-cyano- benzotrofluoride. BCL is rac-bicalutamide. 4-FPMS is 4-fluorophenyl methyl sulfone. DMF is N,N dimethyl formamide. DMSO is dimethyl sulfoxide.
The present invention provides a process for the purification and isolation of bicalutamide. The crude cicalutamide may be prepared by any method, including, for example, the methods disclosed in pending U.S. application serial no. 10/170,271.
The process of the invention comprises the steps of:
(i) combining crude bicalutamide and a solvent; (ii) crystallizing the bicalutamide from the solvent with or without seeding; and (iii) collecting the crystals of bicalutamide. The crude bicalutamide may or may not be substantially soluble in the solvent.
Preferably the crude bicalutamide is soluble in the solvent. Preferably the crude bicalutamide is dissolved in the solvent and the resulting bicalutamide solution or suspension is crystallized by applying agitation for a time sufficient to bring about crystallization of the bicalutamide. The duration of the agitation may be from about 1 hour to about 48 hours. Preferably the duration of the agitation is from about 8 hours to about 15 hours. The agitation may be brought about by any means known to the skilled artisan. The agitation may be accompanied by heating of the reaction mixture. Preferably, the agitation is carried out at room temperature. Preferably the solvent is selected from the group consisting of water, methanol, ethanol, DCM, toluene, PE, chloroform, hexane, 1,2-dichloroethane, diethyl ether, propanol and isopropanol.
According to another embodiment of the invention, the novel process for the purification and isolation of bicalutamide by solution crystallization, comprises the steps of:
(i) combining crude bicalutamide and a first solvent;
(ii) adding a second solvent to the crude bicalutamide-first solvent mixture;
(iii) crystallizing the bicalutamide from the solvents with or without seeding; and (iv) collecting the crystals of bicalutamide.
The first solvent and the second solvents may be the same or different. In one embodiment of the invention, the first and second solvents are the same. Preferably the crude bicalutamide is dissolved in an amount of first solvent sufficient to dissolve the bicalutamide. Preferably the resulting bicalutamide solution is heated to about the boiling point of the first solvent.
Preferably, the amount of second solvent added to the bicalutamide solution is equal to that of the first volume. The second solvent is preferably added under reflux conditions. Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide with or without seeding. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25 °C. The term "seeding" refer to the addition of a crystal of the product to the product solution in order to bring about crystallization, or scratching the inner surface of the crystallization vessel with a glass rod. The present invention covers embodiments where crystallization or precipitation occurs spontaneously, or is induced/accelerated Preferably the first and second solvents are selected from the group consisting of water, methanol, ethanol, ethyl acetate, acetonitrile, acetone, THF, propanol, DMF, DMSO and isobutyl methyl ketone.
In another embodiment, the present invention provides a novel process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining crude bicalutamide and a first solvent;
(ii) adding a second solvent to the crude bicalutamide-first solvent mixture, wherein the second solvent is an anti-solvent; (iii) crystallizing the bicalutamide from the solvents; and
(iv) collecting the crystals of bicalutamide.
Preferably the crude bicalutamide is dissolved in an amount of first solvent sufficient to dissolve the bicalutamide. The the resulting bicalutamide solution is heated to about the boiling point of the first solvent. Preferably the addition of the second solvent, or anti-solvent, takes place under reflux conditions, with the second solvent being added in an amount sufficient to bring about an at least partially desolubilized
bicalutamide. The partial desolubilization is accompanied by the formation of a clouody appearance in the clear solution.
Following addition of the second solvent, a small volume of first solvent, sufficient to dissolve the at least partially desolubilized bicalutamide is added to the mixture.
Sufficient volume of the first solvent is added when the cloudy solution becomes clear.
Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25°C.
Preferably the first solvent: second solvent systems are selected from the group consisting of DMF: water and ethyl acetate: hexane. In another embodiment, the present invention provides a novel process for the purification and isolation of bicalutamide by solution crystallization, comprising the steps of:
(i) combining crude bicalutamide and a first solvent, wherein the first solvent is an anti- solvent; (ii) adding a second solvent to the crude bicalutamide-first solvent mixture;
(iii) crystallizing the bicalutamide from the solvents with or without seeding; and (iv) collecting the crystals of bicalutamide.
Preferably the crude bicalutamide is adedd to the first solvent, or anti-solvent, and the resulting bicalutamide suspension is heated to about the boiling point of the first solvent. The addition of the second solvent, or anti-solvent, takes place under reflux conditions, with the second solvent being added in an amount sufficient to dissolve the bicalutamide. Preferably the crystallizing step comprises cooling the bicalutamide solution to a temperature sufficient to bring about crystallization of bicalutamide. Most preferably the temperature sufficient to bring about crystallization of bicalutamide is about 25°C.
Preferably the first solvent, or anti-solvent is selected from the group consisting of toluene, ether, chloroform, water, methanol and ethanol. Preferably the second solvent is selected from the group consisting of acetonitrile, acetone, THF, DMF and isobutyl methyl ketone.
In another embodiment, the novel processes for the purification and isolation of bicalutamide by solution crystallization of the present invention are carried out using solvents having low toxic potential. Suitable solvents are described as Class III solvents in the ICH Harmonized Tripartite Guideline, Impurities: Guideline for Residual Solvents. Class III solvents are described as being regarded as less toxic and of lower risk to human health, and include no solvent known as a human health hazard at levels normally accepted in pharmaceuticals. Class III solvents acetic acid, acetone, anisole, 1 -butanol, 2- butanol, butyl acetate, tert-butylmethyl ether, cumene, dimethyl sulfoxide, ethanol, ethyl acetate, ethyl ether, ethyl formate, formic acid, heptane, isobutyl acetate, isopropyl acetate, methyl acetate, 3 -methyl- 1 -butanol, methylethyl ketone, 2-methyl-l -propanol, pentane, 1- pentanol, 1 -propanol, 2-propanol, propyl acetate and tetrahydro furan. The process according to our invention is described in detail by the following, but not limiting, examples.
Example 1 Crystallization Methods for Preparation of Purified bicalutamide Example 1A Crude bicalutamide (260 g) was dissolved in ethanol (5 L) at reflux temperature, and water (7.5 L) was added gradually over a period of 1.5-2 hours to precipitate the product. The slurry was cooled to 0-5°C and stirred for 2 hours. The precipitate was collected, washed with water (625 mL), and dried at 60°C in a vacuum oven to yield the crystalline bicalutamide (252.5 6 g, 94% for two steps). Purity: 99.94 % Assay: 99.4%
Water: 0.11% Ethanol: 142 ppm
Example IB A sample of rac-bicalutamide (1.5 g, 3.45 mmol) was dissolved in a minimum volume of a solvent and then boiled. Under reflux conditions, an additional volume of the same solvent was added until the solution was clear and no precipitate was observed. Following the addition of solvent, the solution was cooled to room temperature and left to stand over-night. The crystals were filtered off and dried in an oven at 70°C under vacuum. Solvent systems:
1 Ethyl acetate (20mL)
2 Acetonitrile (5mL) 3 Acetone (7.2mL) 4 THF (7.2mL) 5 n-Propanol (27mL) 6 DMF (2.2mL) 7 DMSO (2.2mL) 8 Isobutyl methyl ketone (2.2mL)
Example IC Bicalutamide was dissolved in a suitable solvent in which it is readily soluble (minimum volume under reflux) and then an anti-solvent was added until a cloudy solution was formed. A few drops of the solvent were then added to clear the solution again and the solution was cooled to room temperature and left to stand overnight. The crystals were filtered off and dried in an oven at 70°C under vacuum. Solvent systems:
1. DMF (12mL): water (18mL)
2. Ethyl acetate (33mL): hexane (6mL)
Example ID A suspension of bicalutamide in an anti-solvent was prepared (fixed volume under reflux) and then a highly solublizing solvent was added until a clear solution was formed and all the precipitate disappeared. The solution was then cooled to room temperature and left to stand overnight .
The crystals were filtered off and dried in an oven at 70°C under vacuum. Solvent systems:
1. Toluene (50mL): acetonitrile (4mL)
2. Ether (50mL): acetonitrile (36mL) 3. Chloroform (50mL): acetonitrile (13mL) Water (lOmL): acetonitrile (14mL) Water (lOmL): acetone (26mL) Water (lOmL): THF (36mL)
7. Methanol (lOmL) acetonitrile (5mL)
8. Methanol (lOmL) acetone (4mL)
9. Methanol (lOmL) THF (4mL)
10. Methanol (lOmL) DMF(12.4mL)
11. Ethanol (lOmL): THF (6.4mL)
12. Ethanol (lOmL): D I MF (12.4mL)
13. Ethanol (lOmL): I Isobutyl methyl ketone (12.4mL)
Example IE
Trituration procedure
A sample of rac-bicalutamide (~1 g, 2.3 mmol) was suspended in a fixed volume (30 mL) of a solvent and stirred vigorously at room temperature overnight.
The crystals were filtered off and dried in an oven at 70°C under vacuum.
Solvent systems:
1. Water (30mL)
2. Methanol (30mL)
3. Ethanol (30mL)
4. DCM (30mL)
5. Toluene (30mL)
6. Petroleum ether (30mL)
7. Chloroform (30mL)
8. n-Hexane (30mL)
9. 1,2-Dichloroethane (30mL)
10. Diethyl ether (30mL)
11. n-Propanol (30mL)
12. Iso-propanol (30mL)