EP1534982A4 - Soupape a microbille monodirectionnelle destinee a un appareil microfluidique - Google Patents

Soupape a microbille monodirectionnelle destinee a un appareil microfluidique

Info

Publication number
EP1534982A4
EP1534982A4 EP03771653A EP03771653A EP1534982A4 EP 1534982 A4 EP1534982 A4 EP 1534982A4 EP 03771653 A EP03771653 A EP 03771653A EP 03771653 A EP03771653 A EP 03771653A EP 1534982 A4 EP1534982 A4 EP 1534982A4
Authority
EP
European Patent Office
Prior art keywords
microfluidic device
microfluidic
passageway
ball element
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03771653A
Other languages
German (de)
English (en)
Other versions
EP1534982A2 (fr
Inventor
David M Cox
Michael P Harrold
Dennis E Mead
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/403,640 external-priority patent/US7201881B2/en
Priority claimed from US10/403,652 external-priority patent/US7135147B2/en
Priority claimed from US10/414,179 external-priority patent/US20040018559A1/en
Priority claimed from US10/426,587 external-priority patent/US6817373B2/en
Application filed by Applera Corp filed Critical Applera Corp
Publication of EP1534982A2 publication Critical patent/EP1534982A2/fr
Publication of EP1534982A4 publication Critical patent/EP1534982A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15C—FLUID-CIRCUIT ELEMENTS PREDOMINANTLY USED FOR COMPUTING OR CONTROL PURPOSES
    • F15C5/00—Manufacture of fluid circuit elements; Manufacture of assemblages of such elements integrated circuits
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K15/00—Check valves
    • F16K15/02—Check valves with guided rigid valve members
    • F16K15/04—Check valves with guided rigid valve members shaped as balls
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
    • F16K99/0023—Constructional types of microvalves; Details of the cutting-off member with ball-shaped valve members
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0034—Operating means specially adapted for microvalves
    • F16K99/0055—Operating means specially adapted for microvalves actuated by fluids
    • F16K99/0057—Operating means specially adapted for microvalves actuated by fluids the fluid being the circulating fluid itself, e.g. check valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/008—Multi-layer fabrications

Definitions

  • the present teachings relate to microfluidic devices, and methods for using such devices. More particularly, the present, teachings relate to devices and methods that allow for the manipulation and processing of micro-sized amounts of fluids in a microfluidic device.
  • Microfluidic devices are useful for manipulating micro-sized fluid samples. There continues to exist a need for devices and methods for achieving quick, simple, reliable, and cost effective fluid manipulation control in microfluidic devices to efficiently process micro-sized fluid samples therein.
  • a microfluidic device including a one-directional microball valve.
  • the one-directional microball valve can be situated within a passageway of the microfluidic device and can control fluid flow between microfluidic features formed in or on the microfluidic device.
  • the microball valve can include at least one ball element that includes an outer-peripheral feature that mates with an inner-peripheral feature of a corresponding valve seat.
  • the valve seat can be situated between an entrance opening and an exit opening of a passageway and can be defined by a portion of the passageway.
  • a microball valve and seat assembly can be disposed in a through hole formed in a substrate.
  • the microfluidic device can include a substrate that includes a pathway formed therein.
  • the pathway can include a passageway with an entrance opening and an exit opening separated by a one-dimensional microball valve.
  • the passageway can be positioned between and in fluid communication with two adjacent but separated microfluidic features of the microfluidic device.
  • the through passageway can taper outwardly between the entrance opening and the exit opening.
  • the entrance opening can have a first niinimum dimension and the exit opening can have a second niinimum dimension that is greater than the first minimum dimension.
  • the minimum dimensions can be diameters.
  • the ball valve element can be unseated from the valve seat by the force of fluid flowing in a direction from the entrance opening of the passageway to the exit opening.
  • the ball valve element can be forcibly seated against the valve seat by way of backpressure or reverse fluid flow caused by fluid disposed downstream of the ball element and flowing in a direction from the entrance opening toward the exit opening.
  • a method for interrupting fluid communication through a microfluidic device by utilizing a one-directional microball valve.
  • the method can include manipulating a fluid to unseat the microball and flow in a direction from the entrance opening toward the exit opening.
  • the microball element can then be caused to seat against the valve seat to at least partially prevent or interrupt fluid flow in a direction from the exit opening toward the entrance opening.
  • the method can include multiple seating and or unseating operations of the microball with respect to the valve seat.
  • FIG. 1 is a perspective view of a valve in a microfluidic device being opened by an external valve opening device
  • FIG. 2 is a top view of a microfluidic device having a plurality of separate sample processing pathways that can each be provided with at least one one-directional microball valve according to various embodiments;
  • Fig. 3 is a cross-sectional side view of a portion of a sample processing pathway that includes a one-directional microball valve according to various embodiments, and through a portion of an exemplary pathway of the many pathways provided in the microfluidic device shown in Fig. 2;
  • Fig. 4 is a cross-sectional side view of the one-directional microball valve shown in Fig. 3 but in an unseated position and suspended by a fluid sample flowing through the valve;
  • Fig. 5 is a cross-sectional side view of the one-directional microball valve of Fig. 3 in a position seated against the corresponding valve seat, for example, by the force of gravity or by back pressure generated by heating a fluid sample; and
  • Fig. 6 is a cross-sectional side view of a portion of a sample processing pathway that includes a microball valve according to various embodiments. DESCRIPTION OF VARIOUS EMBODIMENTS
  • a microfluidic device including a one-directional microball valve.
  • the one-directional microball valve can be situated within a passageway of the microfluidic device and can control fluid flow between microfluidic features formed in or on the microfluidic device.
  • the microball valve can be used to manipulate fluid movement in and through the microfluidic device. Methods for manipulating fluids, that can be practiced in the microball valve microfluidic devices described herein, are exemplified with reference to Fig. 1.
  • Fig. 1 is a perspective view of a valve 11 in a microfluidic device 10, in the process of being opened by an external valve-opening device 9.
  • the external valve-opening device 9 includes an opening blade 12 that can be forced into contact with a cover 20 of the microfluidic device in an area situated between two sample wells, for example, between sample wells 14a, 14b.
  • the cover 20 can include an elasticalfy deformable material, for example.
  • the opening blade 12 can be forced into the cover 20, and can deform a portion of a deformable material forming a substrate 18 of the microfluidic device.
  • the substrate 18 portion to be deformed is located underneath the cover 20 and situated between the two adjacent sample wells 14a, 14b.
  • the cover 20 can at least partially deform back to its initial shape, thereby creating a channel (not shown) at least partially defined by the cover 20 and the deformed material of the substrate 18.
  • the two sample wells 14a, 14b can be placed in fluid communication with one another.
  • the microfluidic device can be spun to centripetally force fluid sample into the radially outwardly arranged sample well 14b, one or more closing blades can be used to manipulate the deformable material of the substrate 18 to form a barrier wall that interrupts fluid communication between the two sample wells 14a, 14b.
  • the fluid sample in the radially arranged outer well 14b can then be processed without the fluid sample undesirably flowing back into the radially inwardly arranged sample well 14a.
  • a one-directional microball valve as described herein can be used in a microfluidic pathway in place of, or in addition to, the deformable valve described above with reference to Fig. 1.
  • An exemplary microfluidic device including a one-directional microball valve according to various embodiments is shown in Figs. 2-5.
  • FIG. 2 is a top view of an exemplary microfluidic device 100 according to various embodiments that can include at least one one-directional microball valve incorporated therein.
  • the microfluidic device 100 can have two input ports 102, 104 for distributing a fluid sample to respective flow distributors 106, 108.
  • Each flow distributor 106, 108 can be in fluid communication with, or be designed to be in valved communication with, a plurality of separate sample processing pathways.
  • the sample processing pathways can each process a respective portion of the fluid sample input into port 102 or 104 as the portion is sequentially moved from the respective flow distributor 106, 108 into the pathways.
  • Fig. 3 is a cross-sectional side view of a portion of a sample processing pathway of the microfluidic device 100 shown in Fig. 2.
  • the cross-section shown in Fig. 3 is taken along an exemplary portion and pathway of the microfluidic device 100 shown in Fig. 2.
  • the microfluidic device 100 can include a substrate, for example, in the form of a layer, 116 having a first surface 122.
  • a cover 112 can be attached to the first surface 122 of the substrate 116 by way of an adhesive, by heat bonding, clamps, fasteners, or the like.
  • Fig. 3 illustrates the use of an adhesive layer 114 for attaching the cover 112 to the first surface 122 of the substrate 116.
  • the microfluidic device 100 can include a second or bottom cover 118 that can be attached to a second or bottom surface 124 of the substrate 116 by way of an adhesive, heat bonding, by clamps, by fasteners, or the like.
  • Fig. 3 illustrates the use of an adhesive layer 120 for attaching the second cover 118 to the second or bottom surface 124 substrate 116.
  • Either or both of the covers 112, 118 can be made from a rigid material, or can be made from a flexible material, such as a polyolefin polymer or rubber, for example.
  • the covers can be elastically deformable and non-brittle.
  • the substrate 116 can be formed as a single-layer of a non-brittle plastic material, such as polycarbonate, or a single-layer made of TOPAZ, a plastic cyclic olefin copolymer material available from Ticona (Celanese AG), Summit, New Jersey, USA.
  • the various wells, chambers, channels, vias, pathways, dividers, valves, and other microfluidic features forming portions of the sample processing pathways, can be formed in the first and second surfaces 122, 124 of the substrate 116, and/or within the body of the substrate 116 of the microfluidic device 100.
  • Methods of manufacturing such features can include using, for example, lithography, stereo-lithography, machining, etching, masking, depositing, or the like techniques.
  • Fig. 2 illustrates an exemplary microfluidic device 100 that includes 96 output chambers 144.
  • Other microfluidic devices can be provided with, for example, 192 or 384 output chambers.
  • the exemplary microfluidic device shown in Fig. 2 can include one or more flow distributors 106, 108, in respective fluid communication with 24 parallel branch channels at the introduction of 24 respective separate processing pathways.
  • Each sample processing pathway can include one or more of the following features: a PCR chamber, a PCR purification chamber, a flow restrictor, a flow-splitter, a forward sequencing chamber, a reverse sequencing chamber, a forward sequencing product purification chamber, a reverse sequencing product purification chamber, a purified forward sequencing product output chamber, a purified reverse sequencing product output chamber, and combinations thereof, in addition to one or more microball valves according to any of the various embodiments described herein.
  • Vias, columns, channels, dividers, vertical flow splitters, and the like can be used to facilitate fluid communication between various microfluidic features formed in or on the substrate 116 of the microfluidic device 100. For example, as best shown in Figs. 3, 4, and 5, channels 128, 130, chamber 132, and through hole 184 can facilitate fluid communication between a flow distributor 106, 108 (not shown in Figs. 3, 4, and 5) and a microfluidic feature, such as a reaction chamber 126.
  • Various structural properties and characteristics of the components of the microfluidic device can be, for example, those described in U.S. Provisional Application No. 60/398,851, filed July 26, 2002, in U.S. Patent Application No. 10/336,330, filed January 3, 2003, and in U.S. Patent Application No. 10/336,274, filed January 3, 2003, all three of which are incorporated herein in their entireties by reference.
  • Figs. 3 to 5 illustrate a side view of a one-directional microball valve 180 for controlling fluid flow through the microfluidic device 100 according to various embodiments, and in sequential stages of operation.
  • the microball valve 180 can include for a passageway a tapered through hole 184 integrally formed in the substrate 116, although any channel, via, duct, passage, hole, depression, or feature can be used.
  • the tapered through hole 184 can be generally conical such that internal surfaces thereof defines a generally conical passageway 190.
  • the tapered through hole 184 can extend longitudinally within the substrate 116 from an entrance opening 192 to an exit opening 194.
  • a longitudinal axis 196 of the tapered through hole 184 can extend substantially perpendicularly to either or both of a first surface 122 and a second surface 124 of the substrate 116. According to various embodiments, a longitudinal axis 196 of the tapered through hole 184 can be angled with respect to either or both of the first surface 122 and the second surface 124 of the substrate 116.
  • the entrance opening 192 of the tapered through hole 184 can have a first minimum dimension, for example, a first diameter, and the exit opening 194 of the tapered through hole 184 can have a second minimum dimension, for example, a second diameter, that is larger than the first minimum dimension.
  • the tapered through hole 184 can taper substantially continuously outwardly from the entrance opening 192 in a direction towards the exit opening 194.
  • the tapered through hole 184 can include an inner sidewall that is formed of the same material as the substratel ⁇ , or can be in the form of a sleeve that is inserted into, onto, or both into and onto the substrate 116.
  • the tapered through hole can provide a valve seat or valve seat surface for a ball element 188.
  • either or both ends of the tapered through hole 184 of the microball valve 180 can be in fluid communication with various microfluidic features, for example, upstream and/or downstream of the microball valve.
  • various microfluidic features for example, upstream and/or downstream of the microball valve.
  • the tapered through hole 184 can be in fluid communication with channels 128, 130, chamber 132, and reaction chamber 126 can be in downstream fluid communication with the tapered through hole 184.
  • other microfluidic features can be in downstream fluid communication with the tapered through hole 184, for example, a sequencing chamber.
  • a ball element 188 can be slideably disposed in the tapered through hole 184.
  • the ball element 188 can be a ball and can have a substantially spherical shape.
  • the ball element 188 can be formed of the same material of the substrate 116 and/or formed of the same material making-up the inner side wall of the tapered through hole 184.
  • the ball element 188 can be metallic, plastic, polymeric, elastomeric, or a combination thereof.
  • the ball element 188 can be made from a relatively soft plastic, such as polycarbonate.
  • the ball element 188 can be spherical, ellipsoidal, ovoidal, retanguloid, cylindrical, conical, cubical, or the like, in shape.
  • the valve seat can have a corresponding peripheral or sealing surface that accommodates and mates with the shape of the ball element 188.
  • the ball element 188 can be dimensioned to slide freely within the tapered through hole 184 and to substantially seal off the entrance opening 192 from the exit opening 194, when seated against a portion of the inner sidewall of tapered through hole 184 which provides a valve seat 198, as shown in Fig. 5.
  • the valve seat 198 can be defined by a portion of the tapered through hole 184, and can have a surface having a substantially circular shape.
  • Flow-through operation of the one-directional microball valve 100 is illustrated further with reference to Fig. 4.
  • a fluid sample is forced to travel through the features of the microfluidic device 100, for example, through channel 128, in a direction towards and through the entrance opening 192, a force exerted by the flowing sample can cause the ball element 188 to unseat from the valve seat 198, and thus become, for example, suspended in a middle portion of the tapered through hole.
  • centripetal force can be used to cause the fluid sample to travel through the microfluidic device 100.
  • Centripetal force can be applied, for example, by securing the microfluidic device to a rotating platen and spinning the platen thereby generating centripetal force that can cause the fluid sample to move in a generally radially outward direction.
  • Arrow 200 in Fig. 4 illustrates the direction of unimpeded flow through the microfluidic device 100 in a generally radially outward direction.
  • the fluid sample can flow around and past the unseated ball element 188.
  • the fluid sample can proceed through the tapered through hole 184 in a direction towards and through the exit opening 194.
  • the fluid sample can be forced to move through one or several additional microfluidic features situated downstream of the exit opening 194.
  • the unlabelled arrows in Fig. 4 illustrate a fluid sample flow path through the exit opening 194, into chamber 132, through a channel 130 and into a reaction chamber 126 for subsequent processing.
  • the reaction chamber 126 can be utilized, for example, as a Polymerase Chain Reaction (PCR) chamber.
  • PCR Polymerase Chain Reaction
  • the force of gravity can cause the ball element 188 to automatically re-seat against the valve seat 198 if the microfluidic device is situated as shown in Figs. 3-5.
  • the ball valve element 188 can prevent, interrupt, or limit fluid communication between the entrance opening 192 and the exit opening 194 of the through hole 184.
  • an increase in temperatures, such as, by the application of heat, in the vicinity of the microball valve 180 can cause the ball element 188 to more tightly seat against the valve seat 198.
  • the fluid sample can be thermally cycled to cause PCR.
  • backpressure can be generated by the heated fluid sample in the reaction chamber 126, thereby forcing the ball element 188 to seat more tightly against the valve seat 198.
  • Arrows 212 in Fig. 5 depict the backpressure applied to the ball element 188.
  • Fig. 5 shows the fluid sample after it has been forced through the one directional microball valve 180 and has been deposited into a microfluidic feature 126, namely a reaction chamber.
  • the reaction chamber 126 can be provided with a ridge 204 separating the reaction chamber 126 from at least the tapered through hole 184.
  • the ridge 204 can operate to prevent the fluid sample from running back, for example, into the tapered through hole 184, once the fluid sample has reached a particular microfluidic feature such as the reaction chamber 126.
  • the ridge 204 can prevent the ball element 188 from undesirably moving into a subsequent microfluidic feature, such as the reaction chamber 126, thus becoming permanently unseated.
  • the ridge 204 can be a barrier.
  • the one-directional microball valve 180 can automatically open and close a fluid communication in a microfluidic device 100 without any external actuation mechanism needed. Moreover, because the need to externally strike or manipulate the microfluidic device 100 can be obviated by the use of the one-directional microball valve 180, better sealing properties can be achieved between the covers 112, 118, and the substrate 116. Proper sealing is desirable, for example, during PCR, when the fluid sample contained in a microfluidic feature of the microfluidic device 100 is thermally cycled.
  • a microfluidic device 500 is provided with a substrate 510 and a fluid flow pathway 520.
  • the pathway 520 includes a microball valve element 530 having a passageway 610 and a ball element 620.
  • the microball element is cubical in shape, and the passageway 610 is a through-hole with a square cross-section.
  • the tapered through hole depicted can instead be a hollow cylindrical passageway that includes, for example, a shoulder and small entrance opening at one end, and a larger exit opening at an opposite end.
  • the one-directional microball valve can be implemented in an existing microfluidic device.
  • a ball element can be inserted into a preexisting tapered through hole in a known microfluidic device to form a microfluidic device according to various embodiments.
  • the ball element can be sized to seat against a valve seat portion of the tapered through hole.
  • an existing microfluidic device having a pre-existing non-tapered through hole can be modified by first tapering the through hole. A ball element can then be inserted into the resultant tapered through hole and can be sized to be seated against the valve seat portion of the tapered through hole.

Landscapes

  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Hardware Design (AREA)
  • Fluid Mechanics (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)
  • Taps Or Cocks (AREA)
  • Fluid-Driven Valves (AREA)
  • Check Valves (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un dispositif microfluidique comportant une soupape à microbille. Ladite soupape à microbille peut empêcher ou interrompre tout flux au travers du dispositif microfluidique. Ledit dispositif microfluidique peut comporter une couche substrat et un passage microfluidique contenant la soupape à microbille. L'invention concerne également des procédés destinés à manipuler des fluides au moyen du dispositif microfluidique.
EP03771653A 2002-07-26 2003-07-17 Soupape a microbille monodirectionnelle destinee a un appareil microfluidique Withdrawn EP1534982A4 (fr)

Applications Claiming Priority (17)

Application Number Priority Date Filing Date Title
US39877802P 2002-07-26 2002-07-26
US39894602P 2002-07-26 2002-07-26
US39885202P 2002-07-26 2002-07-26
US39877702P 2002-07-26 2002-07-26
US398778P 2002-07-26
US398852P 2002-07-26
US398946P 2002-07-26
US398777P 2002-07-26
US10/403,640 US7201881B2 (en) 2002-07-26 2003-03-31 Actuator for deformable valves in a microfluidic device, and method
US403652 2003-03-31
US10/403,652 US7135147B2 (en) 2002-07-26 2003-03-31 Closing blade for deformable valve in a microfluidic device and method
US403640 2003-03-31
US10/414,179 US20040018559A1 (en) 2002-07-26 2003-04-14 Size-exclusion ion-exchange particles
US414179 2003-04-14
US10/426,587 US6817373B2 (en) 2002-07-26 2003-04-30 One-directional microball valve for a microfluidic device
US426587 2003-04-30
PCT/US2003/022470 WO2004011143A2 (fr) 2002-07-26 2003-07-17 Soupape a microbille monodirectionnelle destinee a un appareil microfluidique

Publications (2)

Publication Number Publication Date
EP1534982A2 EP1534982A2 (fr) 2005-06-01
EP1534982A4 true EP1534982A4 (fr) 2006-02-08

Family

ID=31192598

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03771653A Withdrawn EP1534982A4 (fr) 2002-07-26 2003-07-17 Soupape a microbille monodirectionnelle destinee a un appareil microfluidique

Country Status (5)

Country Link
EP (1) EP1534982A4 (fr)
JP (1) JP2006511762A (fr)
AU (1) AU2003253998A1 (fr)
CA (1) CA2488997A1 (fr)
WO (1) WO2004011143A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US7032608B2 (en) * 2004-09-01 2006-04-25 Harris Corporation Microfluidic check-valve embedded in LCP
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
EP2884141A1 (fr) * 2006-08-10 2015-06-17 California Institute of Technology Valve microfluidique présentant un élément flottant libre et son procédé de fabrication
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
ES2582462T3 (es) 2010-09-15 2016-09-13 Minipumps, Llc Válvula de retención de elastómero moldeada y encapsulada
EP2709760B1 (fr) 2011-05-18 2019-06-05 DiaSorin S.p.A. Systèmes et procédés de distribution sur un dispositif de traitement d'échantillons
KR20140022399A (ko) 2011-05-18 2014-02-24 쓰리엠 이노베이티브 프로퍼티즈 컴파니 샘플 처리 장치 상의 체적 계량을 위한 시스템 및 방법
JP6235462B2 (ja) 2011-05-18 2017-11-22 スリーエム イノベイティブ プロパティズ カンパニー 試料処理装置内で材料の選択された体積の存在を検出するためのシステム及び方法
US9845895B2 (en) 2013-01-11 2017-12-19 Minipumps, Llc Diaphragm check valves and methods of manufacture thereof
WO2017047032A1 (fr) * 2015-09-14 2017-03-23 パナソニックＩｐマネジメント株式会社 Appareil à microclapet antiretour
WO2018183744A1 (fr) 2017-03-29 2018-10-04 The Research Foundation For The State University Of New York Dispositif microfluidique et procédés
CN118086053B (zh) * 2024-04-29 2024-07-09 四川迪亚生物科技集团有限公司 一种自灌流培养板及使用方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911616A (en) * 1988-01-19 1990-03-27 Laumann Jr Carl W Micro miniature implantable pump
WO2002097422A1 (fr) * 2001-05-31 2002-12-05 Electron-Bio, Inc. Dispositif microvalve a microbille et son mode de commande
US20030116738A1 (en) * 2001-12-20 2003-06-26 Nanostream, Inc. Microfluidic flow control device with floating element

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US399548A (en) 1889-03-12 nageldinger
US398851A (en) 1889-03-05 Berge
US6595950B1 (en) * 2000-05-11 2003-07-22 Zevex, Inc. Apparatus and method for preventing free flow in an infusion line
US6921253B2 (en) * 2001-12-21 2005-07-26 Cornell Research Foundation, Inc. Dual chamber micropump having checkvalves

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911616A (en) * 1988-01-19 1990-03-27 Laumann Jr Carl W Micro miniature implantable pump
WO2002097422A1 (fr) * 2001-05-31 2002-12-05 Electron-Bio, Inc. Dispositif microvalve a microbille et son mode de commande
US20030116738A1 (en) * 2001-12-20 2003-06-26 Nanostream, Inc. Microfluidic flow control device with floating element

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2004011143A2 *

Also Published As

Publication number Publication date
CA2488997A1 (fr) 2004-02-05
WO2004011143A3 (fr) 2004-09-16
WO2004011143A2 (fr) 2004-02-05
JP2006511762A (ja) 2006-04-06
AU2003253998A1 (en) 2004-02-16
EP1534982A2 (fr) 2005-06-01

Similar Documents

Publication Publication Date Title
US6817373B2 (en) One-directional microball valve for a microfluidic device
WO2004011143A2 (fr) Soupape a microbille monodirectionnelle destinee a un appareil microfluidique
US7737093B2 (en) Liquid processing device including gas trap, and system and method
US7740807B2 (en) Microfluidic device including displaceable material trap, and system
US8012431B2 (en) Closing blade for deformable valve in a microfluidic device and method
JPH0468511B2 (fr)
US20120181460A1 (en) Valves with Hydraulic Actuation System
CA2673844A1 (fr) Ensemble de soupape
TW201003027A (en) Speed regulated pipeline pig
EP2686595B1 (fr) Soupape micro-fluidique et plateforme micro-fluidique
WO2008137986A1 (fr) Système de transfert de fluide quantique
CA3017955A1 (fr) Appareil a cage ayant des passages de fluide pour affecter des caracteristiques d'ecoulement de vannes
DE102011083920A1 (de) Verfahren und vorrichtung zum erzeugen von fluidisch voneinander separierten teilvolumina einer flüssigkeit
WO2001021988A9 (fr) Micro-clapet de non-retour normalement ferme place dans le canal
US20110094591A1 (en) Microfluidic device initialization method, microfluidic device initialization apparatus and microfluidic device package
EP2452110A1 (fr) Dispositifs et systèmes de régulation d'écoulement de fluide, et procédés pour l'écoulement de fluide au travers de ces dispositifs et systèmes
CA3091580A1 (fr) Soupape microfluidique polymere
JP2022519100A (ja) マイクロバルブおよび多方向バルブ器具
JPH09133235A (ja) 三方口逆止弁
US20080087340A1 (en) Primary/secondary loop purge valve
KR100984069B1 (ko) 수동형 미소 유량 제어기
CN113546699A (zh) 用于分配流体的流体设备、装置和方法
JP2006515232A (ja) 微小流体デバイスにおける変形可能な弁のための閉鎖ブレードおよび方法
CN1652880A (zh) 流体产品分配泵
PL70646B1 (fr)

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20050110

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20051222

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20060315